Journal of fish diseases最新文献

{"title":"Molecular Identification, Phylogenetic Analysis, Clinical and Histopathological Findings of Dermocystidium anguillae From Koi Carp (Cyprinus carpio).","authors":"Hooman Rahmati-Holasoo, Sara Shokrpoor, Hadi Ghojaye-Yousefi, Zahra Ziafati Kafi","doi":"10.1111/jfd.70021","DOIUrl":"https://doi.org/10.1111/jfd.70021","url":null,"abstract":"<p><p>Dermocystidium is a pathogenic fungus-like protist that infects many fish species, causing lesions on the skin, gills and other tissues, which may result in systemic disease. In the present study, a new Dermocystidium anguillae infection in the skin and eyes of the koi carp (Cyprinus carpio) from Iran was described using a comprehensive analysis of morphological, histological and molecular data. Filiform hyphal-like cysts were visible on wet mounts. The cysts were filled with spherical to round spores. Wet mount preparations of fresh cyst contents of Dermocystidium anguillae revealed a massive number of spherical to oval spores with refractile bodies. The spores were approximately 5-13 μm in diameter. In histopathological examination, the skin and eye masses were infiltrated by numerous elongated cystic structures. The eosinophilic and hyalinised wall of the cysts was 4-6 μm thick and filled with numerous spherical spores with eccentrically situated nuclei and a large refractile body. In the dermal layer, around the cystic structures, there was edema and granulomatous dermatitis (infiltration of moderate numbers of plasma cells, small lymphocytes and epithelioid cells). Oedema, hyperaemia and multifocal haemorrhages were evident in the ocular masses. The nucleotide sequencing results obtained from the GenBank BLASTN database indicated a 100% sequence similarity with the registered Dermocystidium anguillae.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70021"},"PeriodicalIF":2.2,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disease and Antimicrobial Resistance Surveillance for Nile Tilapia Pathogens in Lake Victoria, Kenya.","authors":"Eric M Teplitz, Venny M Mwainge, Teresia N Wacira, Caleb Ogwai, Meshack J Mayianda, Linnet Ochieng, Ekta Patel, Rodman G Getchell, Christopher M Aura, Kathryn J Fiorella","doi":"10.1111/jfd.70022","DOIUrl":"https://doi.org/10.1111/jfd.70022","url":null,"abstract":"<p><p>The cage aquaculture industry in Lake Victoria, Kenya is growing explosively to meet increasing demand for fish, yet large-scale fish mortalities are prevalent and pose economic shocks with catastrophic livelihood impacts. To gain a more comprehensive understanding of fish health in Lake Victoria, our study applied: (1) survey of cage farmer accounts, perceptions and responses to fish kill events; (2) rapid-response investigation to a significant tilapia mortality event; (3) active disease surveillance; and (4) antimicrobial resistance (AMR) testing of bacterial pathogens via disk diffusion. There were 82 fish kills recalled from 2020 to 2023 with total mortalities exceeding 1.8 million tilapia; yet, only 39% of farmers reported to Kenyan institutions and 17% of farmers attempted medical treatment. Low dissolved oxygen (DO) levels and the isolation of Aeromonas jandaei, Enterobacter hormaechei and Staphylococcus epidermidis were implicated as suspected causes for a recent tilapia mortality event. Active disease surveillance detected trichodinids and monogeneans as common external parasites and identified an additional six bacterial species in tilapia (Acinetobacter soli, Bacillus cereus, Kocuria rhizophila, Micrococcus luteus, Plesiomonas shigelloides, Staphylococcus sciuri) previously published as fish pathogens. Furthermore, we identify AMR patterns that will support the development of host- and pathogen-specific thresholds.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70022"},"PeriodicalIF":2.2,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Silico Assessment of Cannabidiol From Cannabis sativa as an Antiviral Agent Against Key Shrimp Pathogens in Aquaculture.","authors":"Shoba Gunasekaran, Atchuthan Purushothaman, K Anju","doi":"10.1111/jfd.70015","DOIUrl":"https://doi.org/10.1111/jfd.70015","url":null,"abstract":"<p><p>Shrimp aquaculture plays a crucial role in global food production but is increasingly threatened by viral and microsporidian pathogens such as White Spot Syndrome Virus (WSSV), Enterocytozoon hepatopenaei (EHP) and Infectious Hypodermal and Haematopoietic Necrosis Virus (IHHNV). Conventional reliance on antibiotics to combat these infections has raised serious concerns regarding antimicrobial resistance, environmental contamination and food safety. Additionally, environmental stressors such as salinity shifts and poor water quality exacerbate disease outbreaks, leading to severe production losses across Asia and Latin America. To explore eco-friendly therapeutic alternatives, this study assessed the antiviral potential of cannabidiol (CBD), a bioactive compound extracted from Cannabis sativa seed oil, identified through GC-MS analysis. Using molecular docking techniques, we evaluated CBD's interactions with key viral proteins: VP28 of WSSV, the tubulin β-chain of EHP and the capsid protein of IHHNV. The docking results revealed strong binding affinities of -6.61 kcal/mol (EHP), -6.72 kcal/mol (IHHNV) and -5.38 kcal/mol (WSSV), indicating stable and potentially inhibitory interactions. Structural models were retrieved from RCSB PDB and SwissModel, while ligand preparation and docking were performed using AutoDock 4.2. CBD also demonstrated favourable pharmacokinetic and safety profiles, with predictions indicating no mutagenicity, hepatotoxicity or cardiotoxicity, and acceptable drug-likeness characteristics. Compared to other plant-derived compounds previously tested in shrimp disease models, CBD exhibited superior binding stability, more interaction residues and better bioavailability scores. These findings highlight CBD as a promising dual-function agent, capable of both modulating shrimp immunity and directly inhibiting key viral pathogens. These findings highlight cannabidiol (CBD) as a promising dual-action compound, with the potential to both enhance shrimp immune responses and exert direct antiviral effects against key pathogens. This study lays a robust groundwork for future in vivo validations, formulation strategies and regulatory frameworks, ultimately supporting the development of sustainable, precision-based aquaculture health management.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70015"},"PeriodicalIF":2.2,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Iodophor Reduces Carnobacterium maltaromaticum and Vagococcus salmoninarum Prevalence During Brook Trout (Salvelinus fontinalis) Spawning.","authors":"Justine Nelson, Rebekah McCann, Cristina Dahl, Keta Oettinger, Sara Dziki, Ryan Katona, Corey Puzach, Eric Leis, Brandon Keesler, Carey Edwards, Kenneth Phillips, Isaac Standish","doi":"10.1111/jfd.70023","DOIUrl":"https://doi.org/10.1111/jfd.70023","url":null,"abstract":"<p><p>This study examines the effects of iodophor disinfection on the prevalence of Carnobacterium maltaromaticum and Vagococcus salmoninarum during several years of spawning brook trout (Salvelinus fontinalis). The first year of the study, individual eggs were collected from females and separated into four treatment groups: unfertilised, unfertilised and disinfected, fertilised and fertilised and disinfected. Disinfection consisted of iodophor treatment at 100 ppm for 60 min. All eggs were incubated in TSB and monitored for bacterial growth. No growth was observed from disinfected eggs. However, from non-disinfected eggs, C. maltaromaticum was isolated from the surface of 25% of individual unfertilised and 53% of fertilised eggs. V. salmoninarum was only isolated from non-disinfected fertilised eggs. In year two, the process was repeated, though all eggs were fertilised and iodophor disinfected at 100 ppm for 30 min. Again, the pathogens were present on only a small number of disinfected eggs-V. salmoninarum and C. maltaromaticum were detected from the surface of one and five eggs respectively. Throughout the study we found no evidence of intra-ova transmission from disinfected eggs. The study also demonstrates that iodophor is highly efficacious in reducing the prevalence and transmission risk of these pathogens.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70023"},"PeriodicalIF":2.2,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144600643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cyprinid Herpesvirus 2 Infection Activates IL-17C/NF-κB/IFNγ Antiviral Signalling Axis in Caudal Fin Cells of Carassius auratus Gibelio.","authors":"Yaqing Xie, Xiaomi Zhang, Feiran Li, Liqun Lu","doi":"10.1111/jfd.70017","DOIUrl":"https://doi.org/10.1111/jfd.70017","url":null,"abstract":"<p><p>Crucian carp (Carassius auratus gibelio) is sensitive to the infection of Cyprinid herpesvirus 2 (CyHV-2), displaying severe inflammatory reactions characterised by systemic congestion and gill bleeding. This study aimed to elucidate the mechanism by which CyHV-2 infection induces interferon gamma (IFNγ) expression in caudal fin cells (GiCF) of Carassius auratus gibelio, specifically investigating the role of the IL-17C/NF-κB signalling axis. In mammalian systems, virus-induced IL-17 has been demonstrated to activate NF-κB, a master regulator of inflammatory responses that controls multiple antiviral immune genes including interferons. Therefore, the induction mechanism of IFNγ gene expression following CyHV-2 infection was elucidated by revealing the interplay among IL-17C, NF-κB and IFNγ in this study. We demonstrated that CyHV-2 infection significantly upregulated the expression of IL-17C, NF-κB and IFNγ at both transcriptional and translational levels in GiCF. Furthermore, overexpression of IL-17C upregulated NF-κB and IFNγ expressions, while pharmacological inhibition of NF-κB (BAY 11-7082) suppressed this upregulation. This study provides the first evidence for the activation of IL-17C/NF-κB/IFNγ antiviral signalling axis in teleost fish in response to herpesvirus infection. Our findings highlight the dual role of virus-induced NF-κB activity, which not only orchestrates host defence (via IFNγ) but may also be exploited by the virus to potentially regulate its own gene transcription.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70017"},"PeriodicalIF":2.2,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144600642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insect Larvae Meal as a Complementary Functional Ingredient in High Soybean Meal-Based Diets Improve the Health of Rainbow Trout (Oncorhynchus mykiss).","authors":"Jie Ma, Krishna Pada Singha, Mosope F Abanikannda, Veronica Myrsell, Nicholas Romano, Elizabeth Koutsos, Daniel Adams, Kenneth D Cain, Vikas Kumar","doi":"10.1111/jfd.14153","DOIUrl":"https://doi.org/10.1111/jfd.14153","url":null,"abstract":"<p><p>This study explores the integration of black soldier fly larvae (BSFL) as a complementary functional ingredient to increase the soybean meal (SBM) inclusion in rainbow trout (Oncorhynchus mykiss) diets. Six experimental diets were formulated including a control diet as fishmeal (FM), an SBM-based diet, and 2.5% and 5% whole-body (WB) or defatted (DB) BSFL-supplemented SBM-based diets. Results revealed that BSFL inclusion positively influences gut health, immune response and survival rates following challenge with Flavobacterium psychrophilum. Dietary lauric acid content significantly impacted whole-body lauric acid levels in a dose-dependent manner, with BSFL diets showing higher levels than FM and SBM diets. Fish fed diets with BSFL exhibited enhanced survivability against F. psychrophilum infection compared to SBM-fed fish, with the highest survival rates observed in the WB5 (5% whole-body BSFL) group. Histological analysis demonstrated improved intestinal morphology in BSFL-fed fish, particularly evident in the absence of pathogenic enteritis. Gene expression analysis revealed upregulated proinflammatory markers (IL-8, TNF-α, C5) in BSFL-fed fish post-challenge, indicating an enhanced immune response. These findings indicate the potential of BSFL as a functional feed ingredient to complement high SBM inclusion in rainbow trout with improved health and performance. By strategically reducing inclusion levels and repositioning BSFL as a functional feed ingredient instead of fishmeal replacer in aquafeed formulations, one can mitigate economic concerns while maximising the benefits of this sustainable alternative.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14153"},"PeriodicalIF":2.2,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Invitro Evaluation of Bacteriophage Therapy Targeting Streptococcus agalactiae in Nile Tilapia (Oreochromis niloticus): A Potential Approach to Sustainable Disease Management in Aquaculture.","authors":"Nashwa Abdel-Razek, Riad H Khalil, Tamer Mohammed Monir Abdelrahiem, Mohamed Fathi, Sameh A Metwaly","doi":"10.1111/jfd.70019","DOIUrl":"https://doi.org/10.1111/jfd.70019","url":null,"abstract":"<p><p>Streptococcus agalactiae (Group B Streptococcus, GBS) poses a significant threat to Nile tilapia (Oreochromis niloticus) aquaculture, causing severe streptococcosis characterised by high mortality and economic losses. This study elucidates the pathogenesis of S. agalactiae through an integrated diagnostic approach and evaluates bacteriophage therapy as a sustainable alternative to antibiotic treatments. Clinical signs in infected tilapia, including erratic swimming, exophthalmia, and haemorrhagic lesions, were accompanied by systemic postmortem findings such as hepatosplenomegaly and ascitic fluid accumulation. Comprehensive identification of S. agalactiae isolates from pond water and diseased fish was achieved using morphological, biochemical, serological, and molecular techniques, confirming its role as the causative agent. Concurrently, three bacteriophages (STRA1, STRA2, STRA3) were isolated from aquaculture ponds and characterised by their lytic efficacy, host specificity, and environmental stability. These phages exhibited tailed morphologies, high lytic activity (up to 83.3% against S. agalactiae), and resilience under pond-like conditions (pH 4.0-10.0, 28°C-50°C), with STRA1 demonstrating efficacy against multidrug-resistant strains. In vitro assays revealed significant bacterial load reductions (e.g., STRA3: 1.03 × 10⁶ CFU/mL vs. control: 6.13 × 10⁶ CFU/mL at 72 h), though resistant mutants emerged at low frequencies (8.11-8.40 × 10^-4). Suboptimal water quality parameters, including low dissolved oxygen (3.6 mg/L) and elevated iron (1321 μg/L), likely exacerbated infection severity. These findings underscore S. agalactiae's pathogenicity and position bacteriophage therapy as a promising, eco-friendly biocontrol strategy, warranting further in vivo validation to optimise its application in tilapia aquaculture.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70019"},"PeriodicalIF":2.2,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fantastic Flavos—An Introduction to the Special Issue, Flavobacteria: Current Status and Future Directions","authors":"Benjamin R. LaFrentz,&nbsp;Thomas P. Loch,&nbsp;Timothy J. Bruce","doi":"10.1111/jfd.70012","DOIUrl":"10.1111/jfd.70012","url":null,"abstract":"","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":"48 8","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jfd.70012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of a Vaccine for Atlantic Salmon (Salmo salar) Using a Tenacibaculum dicentrarchi Strain Cultured Under Iron-Limited Conditions.","authors":"Ruben Avendaño-Herrera, Rute Irgang, Henry Araya-León, Pedro Ilardi, Raúl Cortés","doi":"10.1111/jfd.70013","DOIUrl":"https://doi.org/10.1111/jfd.70013","url":null,"abstract":"<p><p>Tenacibaculosis, caused by T. dicentrarchi, results in skin lesions, ulceration, yellow plaques on teeth, and haemorrhaging in the operculum, peduncle and pectoral fins. It is the second leading cause of mortality in farmed Atlantic salmon (Salmo salar) in Chile. Currently, no vaccine is available to prevent the disease. In silico and in vivo studies have shown that iron plays a key role in T. dicentrarchi infection. We hypothesised that culturing the TdCh05 vaccine strain under iron-limiting conditions (i.e., with non-assimilable iron chelator 2.2'-dipyridyl [DIP]) would enhance Atlantic salmon protection against tenacibaculosis. Fish were vaccinated intraperitoneally with prototypes (A) TdCh05, (B) TdCh05 + DIP or (C) A + B (a 1:1 mixture of inactivated cultures), and a control group was challenged by bath immersion with a heterologous T. dicentrarchi strain. Each prototype was emulsified with a commercial adjuvant. At 14 days post-challenge, Atlantic salmon injected with FMM broth had a cumulative mortality of 73.3%, followed by the TdCh05 + DIP (66.7%) and A + B (54.8%) prototypes. The lowest cumulative mortality value (50%) was observed for the prototype containing T. dicentrarchi TdCh05 grown only in FMM. Moreover, a high proportion of the vaccinated fish that survived the challenge, regardless of the vaccine prototype, carried T. dicentrarchi in various internal organs, particularly in the spleen. Future studies should focus on identifying the most suitable antigens to develop an effective vaccine for the prevention of T. dicentrarchi-induced tenacibaculosis.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70013"},"PeriodicalIF":2.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a Phage-Display Derived Peptide-Based Colloidal Gold Strip for Rapid Detection of Enterocytozoon hepatopenaei via Spore Wall Protein Interaction.","authors":"Jia-Rong Xie, Hao Li, Ben Chen, Yan-Chang Fan, Yu-Hao Gong, Liqun Lu","doi":"10.1111/jfd.14160","DOIUrl":"https://doi.org/10.1111/jfd.14160","url":null,"abstract":"<p><p>Infection by Enterocytozoon hepatopenaei (EHP) leads to stunted or arrested growth in shrimp, causing significant economic losses in shrimp aquaculture. In this study, the spore wall protein EhSW12 was expressed using a prokaryotic expression system. Subsequently, phage display technology was employed to screen for peptides that interact with EhSW12. A rapid detection method was developed that bypasses the laborious procedure of extracting tissue DNA samples. Instead, enzymatic digestion was used to lyse the spore wall protein present in tissue homogenates, thereby enabling direct detection of EhSW12 via antigen colloidal gold rapid test strips and dot blot assays. The colloidal gold test strips, noted for their convenience and rapidity, satisfy the requirements for on-site testing, while the dot blot method-with its simple operation-is well suited for high-throughput sample analysis. Both methods were compared with the national standard qPCR assay. Specificity was validated using various shrimp antigens prepared in the laboratory; the results demonstrated that only the EHP antigen yielded a positive reaction, with no cross-reaction observed with other common shrimp pathogens (e.g., white spot syndrome virus, decapod iridescent virus and highly pathogenic Vibrio). Sensitivity tests indicated that the minimum detection limits for the test strip and dot blot methods were, respectively, 7 × 10⁶ copies and 5.2 × 10⁴ copies. In summary, both methods exhibit strong specificity, high sensitivity, rapid detection speed and operational simplicity-characteristics that make them highly suitable for large-scale on-site rapid testing.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14160"},"PeriodicalIF":2.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}