Journal of fish diseases最新文献

{"title":"Establishment and Application of a Triple TaqMan qPCR Detection Method for IMNV, DIV1, and CMNV.","authors":"Yuzhi Wang, Chuang Cui, Xiaoqian Tang, Jing Xing, Xiuzhen Sheng, Heng Chi, Wenbin Zhan","doi":"10.1111/jfd.70229","DOIUrl":"10.1111/jfd.70229","url":null,"abstract":"<p><p>The escalating incidence of emerging pathogens and their propensity for co-infection pose a severe threat to the sustainable intensification of Penaeus vannamei aquaculture. To address this challenge, a novel triplex TaqMan quantitative PCR (qPCR) assay was developed for the simultaneous detection of three high-risk agents: Infectious Myonecrosis Virus (IMNV), Decapod Iridescent Virus 1 (DIV1), and Covert Mortality Nodavirus (CMNV). This study not only establishes a robust multiplex platform but also systematically optimises its performance, achieving superior analytical sensitivity and reproducibility compared to existing single-target or duplex methods. The assay demonstrated a limit of detection as low as 10¹ copies/μL for all targets, with excellent inter- and intra-assay coefficients of variation (CVs) consistently below 3%. Notably, the method effectively overcomes the technical hurdles associated with detecting both RNA (IMNV, CMNV) and DNA (DIV1) viruses within a single reaction, eliminating cross-reactivity and ensuring high specificity. Validated against clinical samples, the established protocol exhibits a concordance rate exceeding 95% with current standard methods, while significantly streamlining the diagnostic workflow. This advancement provides a powerful tool for routine biosecurity monitoring, seed stock certification, and the comprehensive management of emerging shrimp diseases.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70229"},"PeriodicalIF":2.3,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148436966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective Systemic Responses During Aeromonas salmonicida Subsp. Salmonicida Infection in Northern Snakehead (Channa argus).","authors":"Ishani Bhgaya Vidana Pallaththarage, Su-Mi Shin, Bhashini Prasadika Waidyarathna, Dong-Jin Lim, Min-Sung Kim, Myung-Hwa Jung","doi":"10.1111/jfd.70312","DOIUrl":"https://doi.org/10.1111/jfd.70312","url":null,"abstract":"<p><p>Northern snakehead (Channa argus) is a bimodal air-breathing fish in which erythrocyte-associated parameters remain relatively stable during physiological challenges such as aerial exposure. However, whether this characteristic is maintained during systemic infection remains unclear. In this study, temporal host responses were characterized following experimental Aeromonas salmonicida subsp. salmonicida infection by integrating haematological, leukocyte morphological, biochemical, and multi-tissue transcriptional analyses. Most erythrocyte-associated indices, including RBC, HGB, HCT, MCV, MCH, and RDW, showed no significant temporal changes, whereas MON, GRA, and PLT exhibited significant responses. Morphologically abnormal leukocytes increased from approximately 7% in healthy fish to 56% at 6 days post-infection and remained above 50% through 8 days, indicating persistent leukocyte-associated cellular alterations during infection. Biochemical responses were also selective, with changes primarily in protein- and metabolism-associated parameters. Transcriptional responses differed among peripheral blood, head kidney, spleen, and liver, including sustained casp7 suppression in the head kidney and distinct inflammatory and apoptosis-associated responses across tissues. Two-way ANOVA further identified significant tissue × time interactions for casp8, tnfα, and casp3. Collectively, A. salmonicida infection in northern snakehead was characterized by comparatively limited temporal variation in most erythrocyte-associated indices, alongside selective leukocyte, biochemical, and tissue-dependent transcriptional responses. These findings highlight a contrasting blood response characterized by persistent leukocyte alterations despite comparatively limited temporal variation in most erythrocyte-associated indices during bacterial infection in northern snakehead.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70312"},"PeriodicalIF":2.3,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infectious Pancreatic Necrosis in Atlantic Cod (Gadus morhua): Complete Viral Genome Characterization From the First Norwegian Field Case Suggests Cross-Species Transmission.","authors":"Anne Berit Olsen, Frieda Betty Ploss, Tore Grøtting, Cathrine Arnason Bøe, Lone Thiel Engerdahl, Inger Austrheim Heffernan, Mona Gjessing, Cecilie Flatnes Nystøyl, Simon Chioma Weli, Bjørn Spilsberg, Ragnhild Tønnessen","doi":"10.1111/jfd.70304","DOIUrl":"https://doi.org/10.1111/jfd.70304","url":null,"abstract":"<p><p>We describe the first natural outbreak of infectious pancreatic necrosis (IPN) in farmed Atlantic cod (Gadus morhua) in Norway. IPN was originally described in young salmonids, but the greatest losses have historically occurred in Atlantic salmon (Salmo salar) following sea transfer despite high vaccination coverage. The number of cases fell drastically in Norway after the introduction of IPN virus (IPNV)-resistant QTL-roe and the reduction of 'house strains'. However, recent reports suggest that IPN is re-emerging as an important health challenge in Norwegian aquaculture. Carrier-state infection is documented in many species of fish, molluscs, and crustaceans. Previously, the disease in farmed cod has only been reported from Denmark and the Faroe Islands (1990s). The present case occurred in juveniles in 2024. The main histopathological finding was exocrine pancreatic necrosis, consistent with IPN in salmonids. Viral antigen in lesions was visualized by immunohistochemistry. IPNV infection was confirmed by real-time RT-PCR and virus isolation. Whole-genome sequencing showed that the NVI-066 isolate clustered closely with IPNV strains typically associated with Atlantic salmon, suggesting cross-species transmission, although the source of infection could not be determined. This study demonstrates that clinically relevant IPN can occur in Atlantic cod under field conditions and extends current understanding of IPNV host range, transmission, and disease expression. The findings are particularly relevant for the expanding cod aquaculture industry, highlighting the need for targeted surveillance, biosecurity, and management strategies.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70304"},"PeriodicalIF":2.3,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149194528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Screening and Cell Cultured-Based Isolation of Carp Edema Virus in Koi Carp (Cyprinus carpio Koi) From Aquarium Shops in Tamil Nadu, India.","authors":"Gani Taju, Seepoo Abdul Majeed, Siva Divyalakshmi, Ganesan Ajay Pathra, Azeez Sait Sahul Hameed","doi":"10.1111/jfd.70305","DOIUrl":"https://doi.org/10.1111/jfd.70305","url":null,"abstract":"<p><p>This study aimed to explore the widespread occurrence of carp edema virus (CEV) in ornamental koi carp available at ornamental shops in Kolathur, Tamil Nadu. The affected koi carps showed various symptoms, such as ulcers, loss of scales, body and fin haemorrhage, fin necrosis, and gill hyperplasia. No known fish cell lines have been reported for the continuous culturing of CEV. To address this constraint, gill (ICG) and heart (SICH) cell lines derived from catla fish were used for the continuous in vitro cultivation of CEV. Virus-specific cytopathic effects (CPE) were similarly observed in all 12 consecutive passages in the infected cell lines. Furthermore, the in vitro replication efficacy of CEV in ICG and SICH cell lines was confirmed by PCR, and the viral copies were quantified using quantitative PCR (qPCR) at different passages. Initially, the levels of CEV copies were very low; however, they peaked by the 12th passage, indicating adaptation to the ICG and SICH cell lines. In addition, to study the virulence of the isolated CEV, filtrate from the pooled organ tissues of fish, as well as the supernatant of ICG culture of CEV infection, was injected into koi carp, and symptoms were observed and mortality was recorded. PCR and qPCR analyses were conducted to assess tissue tropism in various tissues experimentally CEV-infected koi carp. Based on the PCR and qPCR results, gill tissue was identified as the primary target organ for CEV infection. This study proves that CEV replication in fish cell lines supports future vaccine development.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70305"},"PeriodicalIF":2.3,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149182304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Ccombody as an Immunoprophylaxis Strategy: Impact on Immune Gene Expression of Pacific White Shrimp (Penaeus vannamei) Against Acute Hepatopancreatic Necrosis Disease (AHPND).","authors":"Prawporn Thaijongrak, Kasemsuk Kitisriworapun, Theerapat Damrongsakul, Napat Huttayanan, Thitipan Juntong, Andrew P Shinn, Tae Sung Jung, Surasak Jittakhot, Wanna Sirimanapong","doi":"10.1111/jfd.70302","DOIUrl":"https://doi.org/10.1111/jfd.70302","url":null,"abstract":"<p><p>Acute hepatopancreatic necrosis disease (AHPND) is caused by a plasmid bearing strain of Vibrio parahaemolyticus (Vp_AHPND), which produces PirA and PirB toxins that specifically target and damage hepatopancreatic and epithelial cells of the shrimp gastrointestinal tract. The impact of AHPND, together with concerns regarding antibiotic resistance, has necessitated investigations into alternative forms of disease prevention. This study evaluated the protective efficacy of Ccombody, a recombinant variable lymphocyte receptor B (VLRB)-based antibody targeting the PirA toxin, on immune-related gene expression and survival in Pacific white shrimp (Penaeus vannamei) challenged with Vp_AHPND. Shrimp were allocated into six experimental groups, each in triplicate. Two groups served as controls fed a basal commercial diet: an unchallenged negative control and a Vp_AHPND-challenged positive control. An additional reference group was fed a commercial diet supplemented with IgY at 500 mg/100 g feed. The remaining three treatment groups were fed diets supplemented with the Ccombody at 1, 10 or 100 mg/100 g feed for 7 days. At 7 days post-feeding (7 d.p.f.), prophenoloxidase-2 (proPO-2), anti-lipopolysaccharide factors-1 (ALF-1) and penaeidin-4 (PEN4) expression levels showed no significant differences among treatments. At 24 h post-infection (24 h.p.i.), proPO-2 expression of Ccombody groups differed significantly from both the positive and negative control groups (p < 0.05), while no significant differences were observed among Ccombody-treated groups. ALF-1 expression remained unchanged across treatments. In contrast, the Ccombody 1 and 10 mg/100 g feed groups showed significantly enhanced PEN4 expression compared to the positive control group (p < 0.05). Notably, PEN4 expression significantly increased in Ccombody 1 mg/100 g feed group at 24 h.p.i. relative to 7 d.p.f. (p < 0.05), whereas a decline was observed in the positive control and Ig-Guard A groups. Principal component analysis (PCA) also showed the same scattering trend towards gene expression analysis at both 7 d.p.f. and 24 h.p.i. Dietary supplementation with Ccombody 1, 10 and 100 mg/100 g feed effectively reduced the mortality rate following challenge with V. parahaemolyticus causing AHPND. All supplemented groups showed highly significant reduction in mortality compared to the positive control group (p < 0.001). Among them, the 10 and 100 mg/100 g feed groups exhibited the lowest mortality rates (3.70% ± 1.28% and 3.70% ± 3.39%, respectively). When considered alongside the immune-related gene expression, these findings suggest that Ccombody has strong potential as an alternative immunoprophylaxis strategy for the prevention of Vp_AHPND.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70302"},"PeriodicalIF":2.3,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149167038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brequinar Exerts Antiviral Effects Against Aquareovirus via Host Pyrimidine Metabolism Modulation.","authors":"Linyi Liu, Weijun Wu, Sen Han, Liqun Lu","doi":"10.1111/jfd.70300","DOIUrl":"https://doi.org/10.1111/jfd.70300","url":null,"abstract":"<p><p>Grass carp reovirus (GCRV) causes hemorrhagic disease in aquaculture, necessitating novel antivirals. Host-targeted broad-spectrum agents represent a promising approach. We evaluated Brequinar, a dihydroorotate dehydrogenase (DHODH) inhibitor in pyrimidine de novo synthesis, against GCRV in vitro and in vivo. In grass carp kidney (CIK) cells, Brequinar (0-50 μM) showed no cytotoxicity, dose-dependently alleviated cytopathic effect, reduced viral titers and suppressed VP5/VP7 expression. Antiviral efficacy was observed under pre-treatment, post-treatment and 48 h pre-treatment regimens, with post-infection administration showing stronger inhibition at moderate concentrations. In grass carp, Brequinar significantly reduced GCRV-II-induced mortality and tissue viral loads; post-infection dosing conferred better survival. Pharmacokinetic analysis demonstrated markedly greater systemic and tissue exposure following intraperitoneal administration than oral gavage, with peak concentrations reaching 1493.09 ng/mL in blood and 931.13 ng/mL in liver after intraperitoneal injection, compared with only 114.8 and 105.6 ng/mL, respectively, following oral administration. Transcriptomics demonstrated broad downregulation of pyrimidine metabolism genes, validated by sorbitol dehydrogenase (SORD) suppression. In a 30-day rare minnow model, Brequinar reduced viral loads after an initial rise, suggesting its potential application in reducing viral burden in virus-infected broodstock. Collectively, Brequinar exerts anti-GCRV activity by interfering with pyrimidine metabolism via DHODH inhibition and shows potential as a host-targeted antiviral strategy, with possible application in reducing viral burden in high-value broodstock in aquaculture.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70300"},"PeriodicalIF":2.3,"publicationDate":"2026-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149147522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Report of Natural Co-Infection by Gyrodactylus lenoki and Lactococcus garvieae in Farmed Rainbow Trout (Oncorhynchus mykiss): Field and Experimental Evidence for Parasite-Enhanced Mortality, With a COI-Morphology Species Discordance.","authors":"Truong Dinh Hoai, Nguyen Duy Trieu, Doan Thi Nhinh, Dang Thi Hoa, Nguyen Thi Huong Giang, Andrew P Shinn","doi":"10.1111/jfd.70298","DOIUrl":"https://doi.org/10.1111/jfd.70298","url":null,"abstract":"<p><p>It is well established that ectoparasite infection can increase the pathogenicity of, and host susceptibility to, bacterial infections in fish, including rainbow trout; however, no interaction between the ectoparasite Gyrodactylus and the bacterium Lactococcus garvieae has previously been documented. This study presents the first report of a severe natural co-infection of Gyrodactylus lenoki and Lactococcus garvieae in farmed rainbow trout in Vietnam, with mortality rates ranging from 25% to 43% within 4 to 5 days of the first recognised pathogen-associated fish mortality. The identity of both pathogens was established using morphological characterisation, sequencing, and PCR assays, providing clear evidence of dual infection under natural conditions. Molecular (COI) data initially suggested homology with Gyrodactylus salaris, a notifiable pathogen of major regulatory concern that has not previously been recorded outside Europe, but size-invariant morphological analysis of the marginal hook sickles confirmed the parasite as G. lenoki, demonstrating that COI barcode data alone can be misleading for regulatory-critical species identification within this genus. Experimental challenge trials demonstrated that while G. lenoki exhibits low virulence toward rainbow trout on its own, it significantly exacerbates the severity of L. garvieae infection, leading to higher mortality (approximately 25% mortality in fish infected with L. garvieae alone, rising to up to 45% in co-infected fish, with mortality occurring earlier in co-infected groups). Notably, the population dynamics of the ectoparasite varied across experimental groups, with a strong correlation (r = 0.97) observed between host mortality and G. lenoki infection levels. These findings underscore the critical role of ectoparasitic infections in increasing rainbow trout susceptibility to bacterial invasion and highlight the diagnostic risks of relying on molecular barcoding alone when regulatory-notifiable pathogens are suspected.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70298"},"PeriodicalIF":2.3,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149137016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Review of Salmonid Alphavirus: Focus on Viral Survival, Shedding, Detection and Effects of Vaccination.","authors":"Hamish Rodger, Elisabeth Midtbø Vik, Fredrikke Nyborg Formo, Søren Grove, Britt Bang Jensen","doi":"10.1111/jfd.70301","DOIUrl":"https://doi.org/10.1111/jfd.70301","url":null,"abstract":"<p><p>This review of salmonid alphavirus provides a collection of the most recent knowledge on the factors relevant for the transmission and control of the virus including updates on SAV survival, viral shedding, detection, and effects of vaccination as reported in peer-reviewed literature, presentations, theses, and annual reports. SAV can survive in all water types, being the longest in colder marine waters without organic loading. The biophysical properties of SAV indicate a moderate to high robustness. The virus is shed from infected salmon in at least faeces and mucus. There is reduced viral shedding in SAV vaccinates compared to unvaccinated fish. Individual heart or mid-kidney sampling is the fish tissues recommended for screening via RT-qPCR. SAV can be detected in filtered farm water samples earlier than in monthly fish farm samples. However, detection in water does not necessarily mean that fish will become infected. Genomic surveillance in SAV outbreaks is recommended. Inactivated SAV vaccines have been reported to reduce the number of PD outbreaks, reduce mortalities in a PD outbreak, and reduce the percentage of discarded fish at harvest. DNA-based vaccines have also shown good efficacy in terms of growth and mortality in natural PD outbreaks.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70301"},"PeriodicalIF":2.3,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149127721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogenicity Analysis of Co-Infection by Vibrio anguillarum and Rahnella aquatilis Causing Ulcerative Fin Disease in Tawny Puffer (Takifugu flavidus): Pathogen Isolation, Histopathology and Apoptosis Analysis.","authors":"Chenxiao Xi, Ziquan Wang, Guodong Zhang, Lejiang Wang, Baiyu Li, Lin Xu, Long Zhu, Xinghong Xu","doi":"10.1111/jfd.70299","DOIUrl":"https://doi.org/10.1111/jfd.70299","url":null,"abstract":"<p><p>Takifugu flavidus has high edible and medicinal value, and it is one of the pufferfish with high price and good quality in the market. With the expansion of pufferfish farming, the economic losses caused by bacterial diseases are becoming increasingly serious. Among them, ulcerative fin disease is one of the typical diseases. In this study, dominant strains were isolated from T. flavidus suffering from ulcerative fin disease, and artificial infection experiments were conducted using these strains. The dominant strains were identified by colony morphology, physiological and biochemical identification, drug sensitivity test and genome sequencing. The pathological damage and apoptosis of fins, liver, spleen, intestine, stomach and gills were systematically analysed by microscopic observation, transmission electron microscope observation and TUNEL detection. Two dominant strains were identified as Vibrio anguillarum (16E1-4) and Rahnella aquatilis (LB1-1). Artificial infection tests showed that V. anguillarum was more pathogenic than R. aquatilis. The median lethal dose (LD₅₀) for 16E1-4 was 1.11 × 10⁵ CFU/g (body weight), and the LD₅₀ for LB1-1 was 4.58 × 10⁷ CFU/g. The LD₅₀ of co-infection with strains 16E1-4 and LB1-1 was 5.27 × 10⁵ CFU/g. Histopathology showed that co-infection caused serious damage to the fins, liver, spleen, intestines, stomach and gills of the T. flavidus. TUNEL assay showed that co-infection induced apoptosis in these organs. The results not only provide a theoretical basis for the prevention and control of bacterial diseases in T. flavidus culture, but also provide a reference for the prevention and control of similar diseases in other fish.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70299"},"PeriodicalIF":2.3,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149127696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Molecular Detection of Infectious Spleen and Kidney Necrosis Virus (ISKNV) and the Parasite Centrocestus formosanus in the Ornamental Fish Platy (Xiphophorus maculatus) in Chile","authors":"Jaiber J. Solano-Iguaran,&nbsp;Felipe Pontigo,&nbsp;Dennis Cisterna,&nbsp;Carla Anguita,&nbsp;Margarita P. González-Gómez","doi":"10.1111/jfd.70223","DOIUrl":"10.1111/jfd.70223","url":null,"abstract":"<div>\u0000 \u0000 <p>Infectious Spleen and Kidney Necrosis Virus (ISKNV) is a significant fish pathogen historically reported in East and Southeast Asia and increasingly detected in other regions, often in association with the international movement of ornamental fish. <i>Centrocestus formosanus</i> is a digenean trematode of sanitary and economic relevance, with zoonotic potential, that uses snails and fish as intermediate hosts. In this study, ornamental fish representing 24 species were sampled from 13 quarantine facilities across Chile. ISKNV was detected in samples of platy fish (<i>Xiphophorus maculatus</i>) from two quarantine facilities in the Metropolitana district in Chile, both supplied by the same exporter in the State of Florida (United States). <i>C. formosanus</i> metacercariae were also detected in the gills of two ISKNV-positive platy fish. These results represent the first molecular detection of ISKNV and <i>C. formosanus</i> in ornamental fish in Chile and highlight the relevance of targeted surveillance for high-risk pathogens in imported ornamental fish.</p>\u0000 </div>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":"49 11","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148295359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}