Journal of fish diseases最新文献

Short Communication: First Report of Nephrocalcinosis in Aquacultured Brazilian Sardine (Sardinella brasiliensis Steindachner, 1879). 简短交流：巴西沙丁鱼（Sardinella brasiliensis Steindachner, 1879）肾钙沉着病的首次报道。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-10-24 DOI: 10.1111/jfd.70081

Danielle Souza Vieira, Caio Francisco Santana Farias, Marco Shizuo Owatari, Mauricio Laterça Martins, Aline Brum, Caio Magnotti

引用次数: 0

Myxoid Neurothekeoma in Indian Oil Sardine (Sardinella longiceps ) From the Southeast Coast of India. 来自印度东南海岸的印度油沙丁鱼（长尾沙丁鱼）粘液样神经瘤。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-10-22 DOI: 10.1111/jfd.70073

Vijayapoopathi Singaravel, Ayyaru Gopalakrishnan, Nguyen Dinh-Hung

{"title":"Myxoid Neurothekeoma in Indian Oil Sardine (Sardinella longiceps ) From the Southeast Coast of India.","authors":"Vijayapoopathi Singaravel, Ayyaru Gopalakrishnan, Nguyen Dinh-Hung","doi":"10.1111/jfd.70073","DOIUrl":"https://doi.org/10.1111/jfd.70073","url":null,"abstract":"Myxoid neurothekeoma, also known as nerve sheath myxoma, is a rare benign peripheral nerve sheath tumour primarily reported in mammals, with scarce documentation in teleost fishes. This study reports a confirmed case of myxoid neurothekeoma in the Indian oil sardine (Sardinella longiceps ), a commercially significant marine species along India's southeast coast. An adult female specimen exhibiting a fleshy, dome-shaped, slightly pale brownish mass on the dorsal head was examined grossly and subjected to histopathological analysis using haematoxylin and eosin, Alcian blue and Masson's trichrome staining. Immunohistochemical staining for S100 protein confirmed the neural origin of the tumour cells. Microscopic analysis revealed a well-circumscribed lobular neoplasm composed of spindle to stellate cells within a myxoid stroma rich in acid mucopolysaccharides. Tumour lobules were separated by thin collagenous septa, and widespread S100 positivity supported Schwann cell lineage. The lack of mitotic activity, cytological atypia or invasive features supported a diagnosis of benign myxoid neurothekeoma. This case expands the current understanding of peripheral nerve sheath tumours in marine teleosts and contributes to the broader characterisation of rare neoplasms in aquatic species.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70073"},"PeriodicalIF":2.2,"publicationDate":"2025-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145345632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Xanthophoroma in a Striped Burrfish, Chilomycterus schoepfi. 一种条纹刺鱼的黄斑瘤。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-10-22 DOI: 10.1111/jfd.70075

Justin M Stilwell, Rachel Sheffler, Lauren Michaels, Whitney Daniel, Jamie Torres, Alvin C Camus

引用次数: 0

Development and Validation of a Double-Antibody Sandwich ELISA (DAS-ELISA) for Detection of Anguillid Herpesvirus. 鳗鲡疱疹病毒双抗体夹心ELISA （DAS-ELISA）检测方法的建立与验证。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-10-20 DOI: 10.1111/jfd.70071

Hua Chen, Jin-Xian Yang, Jun-Qing Ge

{"title":"Development and Validation of a Double-Antibody Sandwich ELISA (DAS-ELISA) for Detection of Anguillid Herpesvirus.","authors":"Hua Chen, Jin-Xian Yang, Jun-Qing Ge","doi":"10.1111/jfd.70071","DOIUrl":"https://doi.org/10.1111/jfd.70071","url":null,"abstract":"Anguillid herpesvirus (AngHV) is a highly pathogenic agent that causes \"Mucus sloughing and hemorrhagic septicemia disease\" in eels, resulting in high morbidity and cumulative mortality rates. Therefore, accurate diagnosis of AngHV infection is essential for effective clinical management and epidemiological control. In this study, three monoclonal antibodies (mAbs) against AngHV were developed, and their specificity and affinity were validated using Western blotting and indirect immunofluorescence assays. Subsequently, a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was established for the detection of AngHV, employing mAb 10G8-9F4 as the capture antibody and rabbit polyclonal antibody 9NA (which targets AngHV-ORF95) as the detection antibody. Specificity testing indicated no cross-reactivity with other common aquatic viruses, including Koi herpesvirus (KHV), Grass carp reovirus (GCRV), Infectious pancreatic necrosis virus (IPNV), and Large yellow croaker iridovirus (LYCI). The developed assay effectively identified AngHV in various tissues of infected eels, including the heart, liver, spleen, kidney, gills, intestines, muscle, skin mucus, and fins. Compared to quantitative real-time PCR (qPCR), the detection limit of this assay was determined to be 10,000 copies of AngHV, with an 88.89% concordance rate observed in the analysis of clinical samples between the results of DAS-ELISA and qPCR, thereby demonstrating the reliability of this immunoassay for detecting AngHV in field samples. Overall, the DAS-ELISA developed in this study demonstrates high reactivity and specificity, serving as a rapid and effective diagnostic tool for the detection and prevention of AngHV in eel populations.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70071"},"PeriodicalIF":2.2,"publicationDate":"2025-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145336975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Formulation and Immunological Assessment of DNA Vaccines Utilising In Vivo-Induced Antigens Against Nocardia seriolae in Hybrid Snakehead (Channa maculata ♀ × Channa argus ♂). 杂交黑鱼母×黑鱼♂抗诺卡菌DNA疫苗的制备及免疫学评价

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-10-20 DOI: 10.1111/jfd.70074

Xu Kang, Guoquan Chen, Na Li, Yuhao Li, Liqun Xia, Yishan Lu, Ting Huang, Jianlin Chen

{"title":"Formulation and Immunological Assessment of DNA Vaccines Utilising In Vivo-Induced Antigens Against Nocardia seriolae in Hybrid Snakehead (Channa maculata ♀ × Channa argus ♂).","authors":"Xu Kang, Guoquan Chen, Na Li, Yuhao Li, Liqun Xia, Yishan Lu, Ting Huang, Jianlin Chen","doi":"10.1111/jfd.70074","DOIUrl":"https://doi.org/10.1111/jfd.70074","url":null,"abstract":"Nocardia seriolae is the primary cause of fish nocardiosis, and it is urgent to develop an effective vaccine for controlling N. seriolae infection in aquaculture. In our previous study, two potential antigenic proteins, glutamate-cysteine ligase EgtA (EgtA) and hypothetical protein 6747 (hp6747), were determined via the in vivo-induced antigen technique (IVIAT). A DNA vaccine was aimed to be developed using these two in vivo-induced antigens as candidates for ligation with the pcDNA3.1-myc-his-A plasmid, and their immunological effects were evaluated in hybrid snakehead (Channa maculata ♀ × Channa argus ♂). Multiple epitopes of the EgtA and hp6747 proteins from N. seriolae were predicted on both T cells and B cells, and the transcription of the EgtA and hp6747 genes was found in the spleen, trunk kidney, muscle and liver in the fish after immunisation with pcDNA-EgtA and pcDNA-hp6747 DNA vaccines, respectively. Notably, the serum specific antibody (IgM) titres, as well as LYZ, ACP, AKP and SOD activities, were elevated in the vaccinated fish. Meanwhile, quantitative real-time PCR analysis demonstrated that the expression levels of several immune-related genes (TNFα, CD4, CD8α, IL-1β, MHCIIα and MHCIα) were significantly elevated across the aforementioned tissues in the vaccinated fish. Furthermore, they could provide the relative percent survival (RPS) at 61.04% and 43.73% against artificial challenge with N. seriolae in the fish after immunisation with pcDNA-EgtA and pcDNA-hp6747 DNA vaccines, respectively. Taken together, using these two in vivo-induced antigens of EgtA and hp6747 for vaccine development was the ideal strategy for controlling fish nocardiosis in aquaculture.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70074"},"PeriodicalIF":2.2,"publicationDate":"2025-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145337044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First Detection of Lactococcus formosensis subsp. formosensis in Rainbow Trout (Oncorhynchus mykiss) in Europe. 台湾乳球菌亚种首次检出。欧洲虹鳟（Oncorhynchus mykiss）的台湾感病。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-10-11 DOI: 10.1111/jfd.70068

Silvia Colussi, Giuseppe Esposito, Khalid Shahin, Pier Luigi Acutis, Lucio Fariano, Claudio Ghittino, Maria Goria, Fabio Bondavalli, Paolo Ajmone Marsan, Giorgia Bignami, Elena Bozzetta, Marino Prearo, Paolo Pastorino

{"title":"First Detection of Lactococcus formosensis subsp. formosensis in Rainbow Trout (Oncorhynchus mykiss) in Europe.","authors":"Silvia Colussi, Giuseppe Esposito, Khalid Shahin, Pier Luigi Acutis, Lucio Fariano, Claudio Ghittino, Maria Goria, Fabio Bondavalli, Paolo Ajmone Marsan, Giorgia Bignami, Elena Bozzetta, Marino Prearo, Paolo Pastorino","doi":"10.1111/jfd.70068","DOIUrl":"https://doi.org/10.1111/jfd.70068","url":null,"abstract":"Lactococcus garvieae, Lactococcus petauri and Lactococcus formosensis are etiological agents of piscine lactococcosis, a disease reported in Italy since the early 1990s and linked to significant aquaculture losses. To the best of our knowledge, this study reports the first detection of L. formosensis subsp. formosensis in farmed rainbow trout (Oncorhynchus mykiss) in Europe. In 2024, a total of 70 trout were sampled in the Piedmont region (Northern Italy), as part of a health monitoring programme. Bacteriological and molecular analyses showed the presence of L. garvieae in four fish (5.7%) and the identification of L. formosensis subsp. formosensis in one single fish exhibiting mild clinical signs via 16S-23S rRNA ITS sequencing. Phylogenetic analysis confirmed the clustering of the isolate within the L. formosensis clade, clearly distinct from L. garvieae and L. petauri. Virulotype comparison showed greater similarity between L. petauri and L. formosensis subsp. formosensis, while the latter exhibited a distinct biochemical profile and was highly susceptible to antibiotics commonly used in aquaculture. Nevertheless, monitoring and advanced diagnostics are essential to clarify the role of this bacterium and to develop any necessary preventive and control measures.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70068"},"PeriodicalIF":2.2,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145274456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Presence of Adult Cardicola forsteri (Trematoda: Aporocotylidae) in the Gills of Southern Bluefin Tuna: Implications for Diagnosis and Pathology. 南方蓝鳍金枪鱼鳃中成虫的存在：诊断和病理意义。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-10-11 DOI: 10.1111/jfd.70069

Jemma Hudson, Melissa J Carabott, Sho Shirakashi, Nathan Bott, Barbara F Nowak

{"title":"Presence of Adult Cardicola forsteri (Trematoda: Aporocotylidae) in the Gills of Southern Bluefin Tuna: Implications for Diagnosis and Pathology.","authors":"Jemma Hudson, Melissa J Carabott, Sho Shirakashi, Nathan Bott, Barbara F Nowak","doi":"10.1111/jfd.70069","DOIUrl":"https://doi.org/10.1111/jfd.70069","url":null,"abstract":"Blood flukes cause serious health issues for farmed bluefin tuna worldwide. Their infection severity has been assessed by counting adult flukes in heart flushes. However, Cardicola orientalis adults reside in blood vessels in the gills. The aim of this study was to assess species composition, prevalence and intensity of adult blood fluke infection in gill blood vessels of ranched Southern Bluefin Tuna, Thunnus maccoyii. Based on molecular analysis, out of 41 adult blood flukes found in the gills of the tuna, 32 flukes were confirmed to be C. forsteri, while only 1 fluke was C. orientalis. There was a significant relationship between the number of adult C. forsteri in the heart and in the gills, with the heart having significantly greater prevalence. The presence of adult C. forsteri in the gills can cause blockage of the gill blood vessels and have a significant effect on the host. Furthermore, the presence of adult C. forsteri in gill blood vessels may have implications for the diagnosis of the infection and in particular identification of a negative host. The presence of adult C. forsteri in gill blood vessels should be recorded in the species description and considered when assessing the effects of this parasite on the host.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70069"},"PeriodicalIF":2.2,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145274453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Opercular Shortening and Deformities in Farmed Atlantic Salmon (Salmo salar) and Rainbow Trout (Oncorhynchus mykiss)-A Qualitative Study. 养殖大西洋鲑鱼（Salmo salar）和虹鳟鱼（Oncorhynchus mykiss）的眼部缩短和畸形的定性研究。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-10-08 DOI: 10.1111/jfd.70067

Øyvind Salvesen, Grunde Heggland, Ane Grøndahl, Mari Viken Kjønstad, Liv Østevik

{"title":"Opercular Shortening and Deformities in Farmed Atlantic Salmon (Salmo salar) and Rainbow Trout (Oncorhynchus mykiss)-A Qualitative Study.","authors":"Øyvind Salvesen, Grunde Heggland, Ane Grøndahl, Mari Viken Kjønstad, Liv Østevik","doi":"10.1111/jfd.70067","DOIUrl":"https://doi.org/10.1111/jfd.70067","url":null,"abstract":"In the Norwegian aquaculture industry, opercular shortening has been ranked as an important cause of increased mortality, reduced welfare and growth. The aim of this study was to gather experience-based knowledge from fish health personnel and production staff involved in the commercial production of farmed Atlantic salmon and rainbow trout. A total of 29 semi-structured interviews with a predefined list of questions on the topic of opercular shortening were conducted in the period 22.04.24 to 10.12.24. Results showed that participants reported aggressive behaviour due to underfeeding, with subsequent nipping of gill lids as the main cause of shortened opercula in farmed salmonids. Inadequate feed allocation of fry was perceived to be the most important risk factor for aggression, in addition to starving fry. The prevalence of opercular shortening in the freshwater phase was reported to be between 0% and 70%. Opercular shortening was typically first detected in fry between 2 and 5 g, but sometimes already during first feeding (0.5 g) and in alevins. Damaged gill tissue, reduced growth rates and increased mortality were suggested as consequences of shortened opercula. Sufficient feed allocation in fry stages, optimization of environmental conditions, reducing fish density and culling of small fry were reported as preventive measures, while culling affected fish was the sole mitigating measure.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70067"},"PeriodicalIF":2.2,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145251135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome Analysis of Penaeus vannamei Hepatopancreas in Response to Lactococcus garvieae Infection in Low Salinity. 低盐度条件下凡纳滨对虾肝胰腺对garvieae乳球菌感染的转录组分析。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-10-06 DOI: 10.1111/jfd.70065

Rolissa Ballantyne, Jai-Wei Lee, Chun-Hung Liu

{"title":"Transcriptome Analysis of Penaeus vannamei Hepatopancreas in Response to Lactococcus garvieae Infection in Low Salinity.","authors":"Rolissa Ballantyne, Jai-Wei Lee, Chun-Hung Liu","doi":"10.1111/jfd.70065","DOIUrl":"https://doi.org/10.1111/jfd.70065","url":null,"abstract":"Lactococcus garvieae infection significantly affects the health of the white shrimp, Penaeus vannamei, particularly under low salinity conditions (5 ppt). Despite being a euryhaline species, shrimp become more vulnerable to stressors when exposed to low salinity, which can lead to disease outbreaks in aquaculture systems. In this study, we conducted RNA-Seq analysis on hepatopancreatic tissue from shrimp exposed to both low salinity and L. garvieae. The differential transcript analysis revealed 294 transcripts: 234 upregulated and 60 downregulated in shrimp under combined stress conditions. Gene Ontology (GO) analysis indicated that many differentially expressed genes were linked to biological processes, particularly peptidase inhibitory and regulatory activities. Additionally, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the ECM-receptor interaction pathway was upregulated, while pathways related to gluconeogenesis and metabolism were downregulated. The ECM-receptor pathway involved critical genes such as integrin β PS3, laminin, collagen and thrombospondins (THBS). This research provides insights into the molecular responses of shrimp to L. garvieae infection and low salinity stress.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70065"},"PeriodicalIF":2.2,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145232742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fatal Hemorrhagic Septicemia in Common Guitarfish (Rhinobatos rhinobatos) Caused by Photobacterium damselae Subsp. damselae in a Controlled Environment. 由damselae亚种光杆菌引起的致命出血性败血症。在受控环境下的豆娘。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-10-04 DOI: 10.1111/jfd.70066

Giorgia Bignami, Teresa Pirollo, Perla Tedesco, Monica Caffara, Maria Letizia Fioravanti, Elena Campesi, Cristiano Da Rugna, Stefano Gridelli, Francesco Quaglio, Andrea Gustinelli

{"title":"Fatal Hemorrhagic Septicemia in Common Guitarfish (Rhinobatos rhinobatos) Caused by Photobacterium damselae Subsp. damselae in a Controlled Environment.","authors":"Giorgia Bignami, Teresa Pirollo, Perla Tedesco, Monica Caffara, Maria Letizia Fioravanti, Elena Campesi, Cristiano Da Rugna, Stefano Gridelli, Francesco Quaglio, Andrea Gustinelli","doi":"10.1111/jfd.70066","DOIUrl":"https://doi.org/10.1111/jfd.70066","url":null,"abstract":"Elasmobranchs, including sharks and rays, are commonly housed in public aquariums due to their ecological significance and educational value. The common guitarfish (Rhinobatos rhinobatos), currently listed as 'Critically Endangered' by the IUCN, is particularly susceptible to population declines due to overfishing and bycatch. While generally considered robust, individuals in captivity may experience stress-related health issues, increasing their susceptibility to infectious diseases. This study investigates a mortality event affecting three 20-year-old guitarfish kept in a public aquarium. The fish exhibited respiratory distress and ataxia before sudden death. Necropsy findings included external hemorrhages and severe hemorrhagic enteritis. Bacteriological analyses identified Photobacterium damselae subsp. damselae in all specimens through MALDI-TOF and PCR sequencing, while parasitological tests and RT-PCR for Betanodavirus were negative. Histopathology revealed bacterial aggregates in the gills, heart and kidney, consistent with systemic bacterial septicemia. P. damselae subsp. damselae is an opportunistic marine pathogen known to cause hemorrhagic septicemia in various fish species. This case represents the first documented occurrence of fatal P. damselae subsp. damselae septicemia in captive guitarfish. Understanding the impact of infectious diseases in confined environments is essential for improving the health management of endangered elasmobranchs in aquariums and conservation programs.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70066"},"PeriodicalIF":2.2,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145225339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0