Journal of fish diseases最新文献

Isolation, Characterisation and Virulence of Aeromonas spp. From Peruvian Rainbow Trout: First Description of A. popoffii-Associated Mortality. 秘鲁虹鳟鱼气单胞菌的分离、特征和毒力：首次描述了与popoffi相关的死亡率。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-05-29 DOI: 10.1111/jfd.14158

Christopher James Engert, Marco Medina-Morillo, Giovanna Sotil, Jefferson Yunis-Aguinaga

{"title":"Isolation, Characterisation and Virulence of Aeromonas spp. From Peruvian Rainbow Trout: First Description of A. popoffii-Associated Mortality.","authors":"Christopher James Engert, Marco Medina-Morillo, Giovanna Sotil, Jefferson Yunis-Aguinaga","doi":"10.1111/jfd.14158","DOIUrl":"https://doi.org/10.1111/jfd.14158","url":null,"abstract":"Aquaculture is one of the most important food sectors, providing nutrition and employment throughout the world. However, production is under threat due to the occurrence of pathogens. Aeromonas spp. are bacterial agents that affect the aquaculture industry, including rainbow trout (Oncorhynchus mykiss) farms, causing severe economic losses. The objective of this study was to characterise several isolates of Aeromonas spp. obtained from rainbow trout in Junín, Peru. Molecular analysis revealed the presence of four species: A. sobria (n = 12), A. salmonicida (n = 3), A. popoffii (n = 2), and A. media (n = 1). Biochemical characterisation revealed that several isolates tolerated 3% NaCl, with only A. salmonicida surviving in 4% NaCl. Virulence factor analysis demonstrated increased virulence at higher temperatures for A. sobria, while A. popoffii exhibited virulence in colder conditions. A. media displayed limited virulence properties, while A. salmonicida was consistently virulent across all temperatures. Aeromonas salmonicida isolates exhibited intense and prolonged motility-a behaviour rarely associated with this species-that persisted across a broad temperature range from 14°C to 37°C. The virulence genes aer, fla, ahyB, lip, act, ser, hly and altA were present in all A. salmonicida isolates, with both A. popoffii isolates lacking only the fla gene. Experimental infection resulted in mortality of up to 100% for A. salmonicida (7.2 × 106 and 7.2 × 107 CFU/fish), 40% for A. sobria (4 × 107 CFU/fish), and 20% for A. popoffii (4 × 107 CFU/fish). No direct relation was found between specific gene presence or combinations thereof and mortality. Our findings highlight the need to develop effective management strategies, including the development of a multivalent vaccine to enhance Peruvian trout aquaculture sustainability.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14158"},"PeriodicalIF":2.2,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144174142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum Biomarkers in Atlantic Salmon for Differential Diagnosis of Cardiomyopathy Syndrome and Pancreas Disease: Proteomic Identification of Serum Fibrinogen to Enhance Troponin Immunoassay as Optimal Diagnostic Approach. 大西洋鲑鱼的血清生物标志物用于心肌病综合征和胰腺疾病的鉴别诊断：血清纤维蛋白原的蛋白质组学鉴定增强肌钙蛋白免疫测定作为最佳诊断方法。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-05-29 DOI: 10.1111/jfd.14151

Francesca Riva, Lauren Elaine Black, Kim D Thompson, Philippe Sourd, Andrei Bordeianu, Christopher C Chadwick, Hooman Moghadam, Jorge Del-Pozo, Janina Costa, Richard Burchmore, Nicola Brady, Lewis Moore, Suzanne McGill, Mark McLaughlin, Peter David Eckersall

{"title":"Serum Biomarkers in Atlantic Salmon for Differential Diagnosis of Cardiomyopathy Syndrome and Pancreas Disease: Proteomic Identification of Serum Fibrinogen to Enhance Troponin Immunoassay as Optimal Diagnostic Approach.","authors":"Francesca Riva, Lauren Elaine Black, Kim D Thompson, Philippe Sourd, Andrei Bordeianu, Christopher C Chadwick, Hooman Moghadam, Jorge Del-Pozo, Janina Costa, Richard Burchmore, Nicola Brady, Lewis Moore, Suzanne McGill, Mark McLaughlin, Peter David Eckersall","doi":"10.1111/jfd.14151","DOIUrl":"https://doi.org/10.1111/jfd.14151","url":null,"abstract":"Cardiac viral diseases are among the major causes of economic losses in Atlantic salmon (Salmo salar L.) aquaculture. These include cardiomyopathy syndrome (CMS) caused by piscine myocarditis virus (PMCV) and pancreas disease (PD) caused by Atlantic salmonid alphavirus (SAV). The resulting cardiomyopathies impact fish stock in terms of mortality, quality, growth performance and economic loss. Diagnosis of these diseases is currently based on clinical signs, histopathology and RT-qPCR. To identify putative biomarkers for use in the health assessment of Atlantic salmon, a quantitative proteomics investigation was undertaken with the aim of differentiating fish with CMS from healthy fish and fish with PD. Serum samples (n = 9/group) were collected during health assessment from pens where clinical CMS or PD were present and compared to serum from healthy Atlantic salmon. There were 34 differentially abundant proteins (DAPs) in CMS compared to healthy, 66 comparing CMS to PD, and 81 comparing PD to healthy. In relation to healthy samples, most DAPs were shared between CMS and PD, with higher relative abundances observed in PD. An exception to this was serum fibrinogen, which was identified as a putative biomarker for CMS, whereas differentiation of Atlantic salmon with CMS from those with PD was enhanced by the calculation of the ratio of fibrinogen to skeletal troponin C.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14151"},"PeriodicalIF":2.2,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144174120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparing Genome Sequencing Methods to Reconstruct the Spread of Piscine Myocarditis Virus in Ireland. 比较基因组测序方法重建鱼类心肌炎病毒在爱尔兰的传播。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-05-26 DOI: 10.1111/jfd.14150

Andrew J Tighe, Jens Carlsson, Neil M Ruane

{"title":"Comparing Genome Sequencing Methods to Reconstruct the Spread of Piscine Myocarditis Virus in Ireland.","authors":"Andrew J Tighe, Jens Carlsson, Neil M Ruane","doi":"10.1111/jfd.14150","DOIUrl":"https://doi.org/10.1111/jfd.14150","url":null,"abstract":"Piscine myocarditis virus (PMCV), a double-stranded RNA virus, is the causative agent of cardiomyopathy syndrome (CMS) in Atlantic salmon Salmo salar, which was first reported in Ireland in 2012. PMCV continues to be a disease threat to Atlantic salmon aquaculture in Ireland, and as such, it is of growing importance to understand how the virus spreads. Genetic sequences allow for transmission pathways to be examined, with whole genomes providing the most accurate information. In this study, whole genome sequencing has been applied to Irish strains of PMCV, in doing so revealing how > 80% of the genetic diversity of the virus lies outside the commonly sequenced open reading frames (ORFs). Second, this paper examines the effectiveness of incorporating a MinION sequencing approach into routine diagnostics by comparing a MinION generated genome to the corresponding sequence generated via Illumina MiSeq. The results showed the MinION genome shared 99.59% identity with the Illumina genome, and while this accuracy may be sufficient for studies such as pathogen identification and deeper evolutionary questions, it was shown to be insufficient for accurately tracking viral transmission pathways. Finally, comparing Irish and Faroese sequences reveals that some strains of PMCV in Ireland may originate from wild fish.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14150"},"PeriodicalIF":2.2,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recombinant C5a Peptidase and Formalin-Killed Cell: A Synergistic Vaccine Against Streptococcus iniae in Four-Finger Threadfin Fish (Eleutheronema tetradactylum). 重组C5a肽酶和福尔马林杀伤细胞：四指带鱼（Eleutheronema tetradactylum）牛链球菌协同疫苗

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-05-26 DOI: 10.1111/jfd.14154

Andre Giovanni, Yin-Ze Shi, Pei-Chi Wang, Ming-An Tsai, Shih-Chu Chen

{"title":"Recombinant C5a Peptidase and Formalin-Killed Cell: A Synergistic Vaccine Against Streptococcus iniae in Four-Finger Threadfin Fish (Eleutheronema tetradactylum).","authors":"Andre Giovanni, Yin-Ze Shi, Pei-Chi Wang, Ming-An Tsai, Shih-Chu Chen","doi":"10.1111/jfd.14154","DOIUrl":"https://doi.org/10.1111/jfd.14154","url":null,"abstract":"Streptococcus iniae is a pathogenic bacterium that causes severe infections and high mortality rates in threadfin fish farms. Recurrent streptococcosis outbreaks underscore the urgent need for effective vaccination strategies. This study investigates an innovative recombinant protein vaccine incorporating a recombinant C5a peptidase encoding 1780 bp from S. iniae to enhance the immune response of the four-finger threadfin fish (Eleutheronema tetradactylum). Vaccine efficacy was assessed by evaluating specific antibody titres, lysozyme activity and the immune-related gene expression in a challenge test. The results demonstrated that fish immunised with C5a, FKC and C5a + FKC showed significantly higher specific antibody titres and lysozyme activity than the control group. Notably, rC5a + FKC displayed the highest IgM levels against the C5a antigen at 28 dpv expression. The rC5a-based vaccine significantly increased TNFα, IL-10 and IL-12 one day after immunisation, correlating with elevated serum antibody levels and the upregulated immune-related genes (C2, CD8). Fish immunised with the rC5a + FKC vaccine exhibited a stronger immune response and greater protective efficacy against the highly virulent S. iniae strain, with a relative survival rate of 97.5%. The combination rC5a + FKC RPS results are higher than those of the single antigens FKC (87.5%) and rC5a (30%), highlighting the potential of the rC5a and FKC vaccine combination as a promising method for preventing and treating S. iniae infections in threadfin fish, supporting healthier fish stocks and sustainable aquaculture practices.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14154"},"PeriodicalIF":2.2,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Streptococcus iniae Infection on Gut Microbiome Diversity and Immune Response in Four-Finger Threadfin (Eleutheronema tetradactylum). 牛链球菌感染对四指线虫肠道菌群多样性和免疫应答的影响

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-05-23 DOI: 10.1111/jfd.14149

Ashish Patige Madhusudhan, Sudarshan Pandey, Pei-Chi Wang, Shih-Chu Chen, Omkar Vijay Byadgi

{"title":"Impact of Streptococcus iniae Infection on Gut Microbiome Diversity and Immune Response in Four-Finger Threadfin (Eleutheronema tetradactylum).","authors":"Ashish Patige Madhusudhan, Sudarshan Pandey, Pei-Chi Wang, Shih-Chu Chen, Omkar Vijay Byadgi","doi":"10.1111/jfd.14149","DOIUrl":"https://doi.org/10.1111/jfd.14149","url":null,"abstract":"Streptococcus iniae is one of the key etiological agents of Streptococcosis and is a significant pathogen affecting four-finger Threadfin (Eleutheronema tetradactylum), leading to severe clinical manifestations, such as ascitic fluid accumulation in the abdominal cavity and intestinal haemorrhages. Understanding the interplay between fish infections, gut microbiota dysbiosis and immune responses is critical for effective management in aquaculture. This study investigates the impact of the acute infection caused by S. iniae on the gut microbiome composition and immune response of Threadfin fish. Following experimental infection, we observed significant differences in taxonomic richness and diversity between healthy and infected fish during the acute phase of the disease. The gut microbiota was primarily dominated by Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes. Notably, infection led to a marked decline in microbiota richness and diversity in the gut, accompanied by an increase in the relative abundance of pathogenic genera such as Streptococcus and Vibrio. Immune modulation, as evidenced by altered gene expression of selected immune parameters, was observed in tissue samples following clinical infection. Also, histological examinations revealed significant disruptions to intestinal villi architecture in infected fish. These findings suggest that S. iniae alters the gut microbiota composition, which may compromise immune function and elevate the risk of intestinal inflammation. This research underscores the importance of monitoring gut microbiota dynamics as a potential diagnostic tool for managing bacterial infections in aquaculture settings.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14149"},"PeriodicalIF":2.2,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diving Into the Depths: Unveiling the Main Etiologies of Piscine Lactococcosis With a Novel Multiplex qPCR Assay. 潜入深处：用一种新的多重qPCR方法揭示鱼乳球菌病的主要病因。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-05-22 DOI: 10.1111/jfd.14147

Khalid Shahin, Mostafa Abdel-Glil, Izzet Burçin Saticıoğlu, Muhammed Duman, Soner Altun, Silvia Colussi, Giuseppe Esposito, Pier Luigi Acutis, Prearo Marino, Brandon Spondler, Ilhan Altinok, Charalampos Kotzamanidis, Ana Isabel Vela, Esteban Soto, Carlos Augusto Gomes Leal, Nihed Ajmi, Sergio Aoki

{"title":"Diving Into the Depths: Unveiling the Main Etiologies of Piscine Lactococcosis With a Novel Multiplex qPCR Assay.","authors":"Khalid Shahin, Mostafa Abdel-Glil, Izzet Burçin Saticıoğlu, Muhammed Duman, Soner Altun, Silvia Colussi, Giuseppe Esposito, Pier Luigi Acutis, Prearo Marino, Brandon Spondler, Ilhan Altinok, Charalampos Kotzamanidis, Ana Isabel Vela, Esteban Soto, Carlos Augusto Gomes Leal, Nihed Ajmi, Sergio Aoki","doi":"10.1111/jfd.14147","DOIUrl":"https://doi.org/10.1111/jfd.14147","url":null,"abstract":"Piscine lactococcosis poses a significant threat to a wide range of cultured and wild fish populations worldwide, typically presenting as acute haemorrhagic septicemia with high morbidity and mortality. Although Lactococcus garvieae was historically considered the sole causative agent of piscine lactococcosis, recent studies have identified L. petauri and L. formosensis as additional, highly pathogenic species. In this study, we developed a novel TaqMan-based multiplex qPCR assay for the simultaneous detection and differentiation of L. garvieae, L. petauri and L. formosensis, following a pangenome analysis of the publicly available genomes of these bacterial species. The assay demonstrated high sensitivity and specificity across 156 bacterial isolates obtained from various cultured fish species and geographical locations between 2008 and 2024, as well as against a panel of non-target bacteria. It also successfully detected target pathogens in 146 field tissue samples, including tissues preserved in 70% ethanol, formalin-fixed paraffin-embedded (FFPE) tissues and tissues fixed on FTA cards. Compared to classical bacteriology, the multiplex qPCR assay yielded higher detection rates and enabled precise identification of the causative species of piscine lactococcosis. Overall, the multiplex qPCR assay developed in this study provides a reliable, rapid, highly sensitive and species-specific molecular approach for diagnosing piscine lactococcosis, contributing to better surveillance and management of the disease in aquaculture.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14147"},"PeriodicalIF":2.2,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrastructural Insights Into a Candidatus Parilichlamydia sp. Infection of Gill Goblet Cells in Greater Amberjack. 大琥珀鳃杯状细胞感染副鳃原体候选菌的超微结构研究。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-05-21 DOI: 10.1111/jfd.14146

Maria Chiara Cascarano, Papadogiorgaki Sevasti, Pantelis Katharios

{"title":"Ultrastructural Insights Into a Candidatus Parilichlamydia sp. Infection of Gill Goblet Cells in Greater Amberjack.","authors":"Maria Chiara Cascarano, Papadogiorgaki Sevasti, Pantelis Katharios","doi":"10.1111/jfd.14146","DOIUrl":"https://doi.org/10.1111/jfd.14146","url":null,"abstract":"Despite recent genomic studies and increased molecular data, epitheliocystis remains an enigmatic fish disease with no experimental in vitro or in vivo models to aid the advancement of research. In this study, we revert to a classical microscopical approach and screen with the electron microscope the epitheliocystis lesions caused by a Ca. Parilichlamydia sp., infecting mucus cells in Greater amberjack. We report distinct morphological features of this bacterial family, characterised by Intermediate Bodies that closely resemble those of previously described Candidatus similchlamydia, and Elementary Bodies that exhibit morphological similarities to Chlamydia trachomatis. We describe the characteristics of a novel Chlamydial Inclusion Membrane (IM) type, with abundant interdigitations, possibly shaped by fusion of the IM with cytoplasmic vesicles, and moreover discuss the presence of multivesicular bodies in the infected cell. Our observation of immune cells in the infected areas indicates an interaction of macrophages with infected cells, a role for granular cells as pathogens reservoirs and an active phagoptosis process in the nearby areas, overall shedding light on cellular immune processes characterising these infections in fish hosts.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14146"},"PeriodicalIF":2.2,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transmission of Spring Viraemia of Carp Virus Between Common Carp and Nile Tilapia by Cohabitation. 鲤与尼罗罗非鱼同居传播鲤病毒春季病毒血症的研究。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-05-14 DOI: 10.1111/jfd.14144

Esteer Ragheb Fouad, Ebtsam Sayed Hassan Abdallah, Ahmad A Elkamel

{"title":"Transmission of Spring Viraemia of Carp Virus Between Common Carp and Nile Tilapia by Cohabitation.","authors":"Esteer Ragheb Fouad, Ebtsam Sayed Hassan Abdallah, Ahmad A Elkamel","doi":"10.1111/jfd.14144","DOIUrl":"https://doi.org/10.1111/jfd.14144","url":null,"abstract":"The purpose of the current study was to investigate the possible transmission of spring viraemia of carp virus (SVCV) between common carp (Cyprinus carpio) and Nile tilapia (Oreochromis niloticus) through cohabitating infected fish of one species with healthy fish of the counter species. The ability of infected O. niloticus to transmit the infection to nearby C. carpio for an extended period was also evaluated. The cohabitation ratio was one SVCV-challenged fish of one species to three healthy fish of the other species. Epithelioma Papulosum Cyprini cell line was utilised for virus isolation, while RT-PCR was used for virus confirmation. Results showed that the virus can be isolated from and confirmed in various organs of both C. carpio and O. niloticus cohabitated from the first day post cohabitation (DPC) and up to the 17th-day DPC from the gills, while up to the 35th DPC from the visceral organs and intestine. Additionally, the SVCV was successfully detected in the gills and visceral organs of C. carpio cohabitated with O. niloticus infected 14 or 21 days before cohabitation. Results indicated that SVCV can be transmitted from infected O. niloticus or C. carpio to nearby healthy fish of the counter species.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14144"},"PeriodicalIF":2.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143968792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and Genomic Characterisation of Lymphocystivirus platichthys1 in Brackish-Water Cultured Spotted Seabass Lateolabrax maculatus in Zhuhai, South China. 珠海市淡咸水养殖斑点海鲈淋巴囊病毒platichthys1的分离及基因组特征分析

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-05-14 DOI: 10.1111/jfd.14139

Hongling Ma, Changhong Cheng, Yiqin Deng, Zhixun Guo, Yong Li, Guangxin Liu, Huajian Lin, Liwen Xu, Juan Feng

{"title":"Isolation and Genomic Characterisation of Lymphocystivirus platichthys1 in Brackish-Water Cultured Spotted Seabass Lateolabrax maculatus in Zhuhai, South China.","authors":"Hongling Ma, Changhong Cheng, Yiqin Deng, Zhixun Guo, Yong Li, Guangxin Liu, Huajian Lin, Liwen Xu, Juan Feng","doi":"10.1111/jfd.14139","DOIUrl":"https://doi.org/10.1111/jfd.14139","url":null,"abstract":"Lymphocystis disease virus (LCDV) stands as one of the most widespread causative agents affecting a diverse array of teleost globally. According to the literature, LCDV encompasses four species, and Lymphocystivirus paralichthys1 (known as LCDV-C) has long been regarded as the predominant national epidemic isolate in mainland China. In this study, two instances of severe mass mortalities associated with LCDV in brackish-water cultured juvenile spotted seabass in Zhuhai, South China, were monitored starting from early April 2023. The death toll in these two cases peaked at approximately 4000 and 10,000 fish per day, respectively, with cumulative mortalities reaching around 70% within 19 days of the disease outbreak. Based on clinical symptoms, histopathological analyses and transmission electron microscopy observations, the diseases were diagnosed as LCDV infections. The virus was isolated using the Mandarin fish fry (MFF-1) cell line and named LCDV-ZH04/23. Subsequently, LCDV-ZH04/23 was roughly purified, and its entire genome was sequenced and annotated. The results indicated that ZH04/23 consists of 102,661 base pairs, encodes 108 putative viral genes and has a G/C content of 29.3%. Genomic collinearity analysis revealed that the whole genome of ZH04/23 exhibits the highest similarity (99.9%) to the well-known Lymphocystivirus platichthys1 (LCDV-1) from Europe, but shows significant divergence from the other three LCDV species. Collectively, this study marks the first isolation and characterisation of L. platichthys1 at the whole genome level from cases of severe mass mortalities in pond-cultured spotted seabass in China. Moreover, it is the first to identify L. platichthys1 as an emerging pathogen in cultured spotted seabass.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14139"},"PeriodicalIF":2.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143974273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immune Response and Protective Efficacy of β-Glucan and Alkoxyglycerol as Adjuvant in Streptococcus agalactiae Formalin-Inactivated Vaccine in Nile Tilapia (Oreochromis niloticus). β-葡聚糖和烷氧基甘油作为无乳链球菌福尔马林灭活疫苗佐剂对尼罗罗非鱼的免疫应答及保护作用

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-05-14 DOI: 10.1111/jfd.14141

Ritam Guha, K Nandhakumar, Omkar Vijay Byadgi, Shih-Chu Chen, Preetham Elumalai

{"title":"Immune Response and Protective Efficacy of β-Glucan and Alkoxyglycerol as Adjuvant in Streptococcus agalactiae Formalin-Inactivated Vaccine in Nile Tilapia (Oreochromis niloticus).","authors":"Ritam Guha, K Nandhakumar, Omkar Vijay Byadgi, Shih-Chu Chen, Preetham Elumalai","doi":"10.1111/jfd.14141","DOIUrl":"https://doi.org/10.1111/jfd.14141","url":null,"abstract":"Nile tilapia (Oreochromis niloticus) farms face streptococcus agalactiae infections causing mass mortality following economic losses. To date, no commercial fish vaccine is available in India to protect against streptococcosis due to a lack of proper adjuvants and vaccine targets. Multiple studies justify that immunomodulators like β-glucan and Alkoxy glycerol (AKG) have improved immunity in fish. β-glucan and AKG are natural homopolymers and lipids, respectively, which have proven immunostimulant capabilities in fish and can be effectively used as a prominent adjuvant to improve vaccine efficacy. The present study aims to demonstrate the improved adjuvant efficacy of the formalin-inactivated S. agalactiae vaccine. Nile tilapia fingerlings were immunised with formalin-inactivated S. agalactiae vaccines mixed with β-glucan and AKG. Each week postvaccination, the serum nonspecific responses were measured by lysozyme, myeloperoxidase, SOD and bactericidal activity. Following the challenge after 28 days postvaccination, the survival rate achieved was 32.14%, 57.14%, 53.57% and 71.43% in the respective experimental groups. The immune gene expressions of IgM, TCRβ and MHCII were evoked postvaccination. The specific serum IgM titre values were significantly upregulated in 14- and 28-day postvaccinated experimental groups. The study highlights the enhanced activity of β-glucan and AKG incorporated vaccine, which has the potency towards commercialisation for sustainable aquaculture.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14141"},"PeriodicalIF":2.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0