Journal of fish diseases最新文献_第2页

Expanding the Fish-Brain Invitrome With the Senegalese Sole SsB-1 Cell Line-A Versatile Model for Neurotropic Virus Research. 用塞内加尔唯一SsB-1细胞系扩展鱼脑内胚——嗜神经病毒研究的通用模型

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2026-04-28 DOI: 10.1111/jfd.70197

Yulema Valero, Alberto Cuesta

{"title":"Expanding the Fish-Brain Invitrome With the Senegalese Sole SsB-1 Cell Line-A Versatile Model for Neurotropic Virus Research.","authors":"Yulema Valero, Alberto Cuesta","doi":"10.1111/jfd.70197","DOIUrl":"https://doi.org/10.1111/jfd.70197","url":null,"abstract":"Cell lines are essential tools for studying animal physiology and immunopathology, reducing the use of live animals and supporting the 3Rs principle of reduction, refinement, and replacement. The Senegalese sole (Solea senegalensis), a relevant species for Spanish aquaculture diversification, lacks established in vitro models. We developed SsB-1, the first brain-derived cell line from this species, providing a valuable platform for studying virus-host interactions. SsB-1 exhibits glial features, stable morphology, and high susceptibility to several betanodavirus (NNV) genotypes, enabling detailed analysis of neurotropism and virulence mechanisms. Its selective permissiveness to NNV and limited replication of infectious pancreatic necrosis virus (IPNV), spring viremia of carp (SVCV), and rhabdovirus eel virus European X (EVEX) highlight its versatility. The ability of some viruses to enter cells without triggering cytopathic or antiviral responses also points to new research avenues for potential reservoir roles in flatfish. Overall, SsB-1 offers a robust, ethical, and versatile model to advance virological and immunological research in support of sustainable marine aquaculture.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70197"},"PeriodicalIF":2.2,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147773412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Dietary Yeast Nucleic Acids and Free Nucleotides on Growth Performance, Digestive Enzyme Activities, Immune Parameters, Tolerant to Hypoxic Stress and Disease Resistance in Juvenile Pacific White Shrimp (Litopenaeus vannamei). 饲料中添加酵母核酸和游离核苷酸对凡纳滨对虾（Litopenaeus vannamei）幼虾生长性能、消化酶活性、免疫参数、耐缺氧应激和抗病能力的影响

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2026-04-28 DOI: 10.1111/jfd.70195

Thanakrit Ratanaprapaporn, Arunothai Keetanon, Natnicha Chongprachavat, Pakawat Poldetch, Marcelo Gurgel Borba, Stefano Lissoni, Tirawat Rairat, Niti Chuchird

{"title":"Effects of Dietary Yeast Nucleic Acids and Free Nucleotides on Growth Performance, Digestive Enzyme Activities, Immune Parameters, Tolerant to Hypoxic Stress and Disease Resistance in Juvenile Pacific White Shrimp (Litopenaeus vannamei).","authors":"Thanakrit Ratanaprapaporn, Arunothai Keetanon, Natnicha Chongprachavat, Pakawat Poldetch, Marcelo Gurgel Borba, Stefano Lissoni, Tirawat Rairat, Niti Chuchird","doi":"10.1111/jfd.70195","DOIUrl":"https://doi.org/10.1111/jfd.70195","url":null,"abstract":"Yeast nucleic acids and free nucleotides (yeast RNA + NT) is a functional feed additive with health-promoting effect. This study aimed to evaluate the effect of dietary yeast RNA + NT (from Kluyveromyces fragilis) on the health conditions, hypoxic stress tolerance and disease resistance in juvenile Pacific white shrimp (Litopenaeus vannamei). Shrimp were fed diets supplemented with 0, 0.25, 0.50 and 0.75 g·kg-1 yeast RNA + NT for 45 days, followed by challenges with hypoxic stress and Vibrio parahaemolyticus (AHPND). Results showed that yeast RNA + NT supplementation at 0.50 g·kg feed-1 could significantly improve body weight (3.93 g), FCR (1.20), trypsin activity (78.02 mU·mg protein-1) and trypsin-to-chymotrypsin ratio (5.03) compared to the control (3.34 g, 1.59, 64.70 mU·mg protein-1 and 3.78, respectively). All three yeast RNA + NT groups had significantly higher survival rates and lower Vibrio spp. counts in the hepatopancreas. The highest immune responses were observed in the yeast RNA + NT at 0.75 g·kg feed-1 group. Survival rates under hypoxia were significantly higher in the yeast RNA + NT at 0.50 (81.67%) and 0.75 g·kg feed-1 (80.00%) compared to the control (45.00%). Notably, the 0.75 g·kg-1 group exhibited the highest resistance to AHPND, with a survival rate of 65.00% compared to 43.33% in the control. These findings suggest that 0.50 g·kg-1 is optimal for growth promotion, whereas 0.75 g·kg-1 maximises immune function and resistance to hypoxia and bacterial infection.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70195"},"PeriodicalIF":2.2,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147773314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toxin Secretion Systems Used by Marine Pathogenic Bacteria: A Review. 海洋致病菌毒素分泌系统研究进展

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2026-04-27 DOI: 10.1111/jfd.70194

Melissa Camberos-Solano, Sonia A Soto-Rodriguez

{"title":"Toxin Secretion Systems Used by Marine Pathogenic Bacteria: A Review.","authors":"Melissa Camberos-Solano, Sonia A Soto-Rodriguez","doi":"10.1111/jfd.70194","DOIUrl":"https://doi.org/10.1111/jfd.70194","url":null,"abstract":"Diseases caused by marine bacteria pose a significant challenge for aquaculture and related activities, resulting in high mortality rates and substantial economic losses. This review presents a list of bacterial pathogens found in cultured aquatic organisms, along with the secretion systems they use to export virulence factors or toxins. We focus on the principal secretion systems employed by pathogenic marine bacteria, particularly those belonging to the genera Vibrio, Aeromonas, Photobacterium and Tenacibaculum. We describe the structural organization, functional mechanisms and toxin-associated substrates of secretion systems from types I, II, III, IV, VI and IX. Furthermore, we explain the mechanisms of action of the most important marine bacterial toxins, focusing on hemolysins, pore-forming toxins, enzymes and protein toxins, with particular emphasis on the PirAB toxin, which causes acute hepatopancreatic necrosis disease (AHPND). Additionally, we discuss the regulatory networks that control toxin expression in response to environmental conditions and quorum-sensing signals, as well as the impact of the toxins secreted by marine organisms on aquaculture systems. Understanding the mechanisms of toxin secretion in aquaculture is essential for developing effective prevention measures and improving overall health management in aquatic production systems.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70194"},"PeriodicalIF":2.2,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147773448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Two Multienzyme Isothermal Rapid Amplification Techniques for the Rapid Visual Detection of Micropterus salmoides rhabdovirus. 两种多酶等温快速扩增技术快速检测鲑鱼小翼虫横纹肌病毒的建立。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2026-04-21 DOI: 10.1111/jfd.70191

Qingyue Xu, Yiping Lin, Yixia Ma, Zhantian Liao, Tao Li, Shuai Wang, Chunguo Liu, Zhan Luo, Qingqing Song, Shun Zhou, Yunji Xiu

{"title":"Development of Two Multienzyme Isothermal Rapid Amplification Techniques for the Rapid Visual Detection of Micropterus salmoides rhabdovirus.","authors":"Qingyue Xu, Yiping Lin, Yixia Ma, Zhantian Liao, Tao Li, Shuai Wang, Chunguo Liu, Zhan Luo, Qingqing Song, Shun Zhou, Yunji Xiu","doi":"10.1111/jfd.70191","DOIUrl":"https://doi.org/10.1111/jfd.70191","url":null,"abstract":"Largemouth bass (Micropterus salmoides) is an important freshwater-cultured species in China. M. salmoides rhabdovirus (MSRV) is a devastating pathogen that causes high mortality (exceeding 90% in acute outbreaks) in largemouth bass fry, posing a severe threat to the global largemouth bass aquaculture industry. To address the urgent demand for rapid, on-site MSRV detection, two multienzyme isothermal rapid amplification (MIRA)-derived methods-multienzyme isothermal rapid amplification with fluorescence detection (MIRA-FLU) and multienzyme isothermal rapid amplification with lateral flow dipstick (MIRA-LFD)-were established and optimized targeting the conserved nucleoprotein (N) gene of MSRV. The performance of both methods was systematically evaluated, and their practical applicability was verified using clinical samples collected from diseased fish farms. The results demonstrated that both methods exhibited high sensitivity, with a detection limit of 9 copies/μL for the standard plasmid. Both detection methods demonstrated high specificity and showed no cross-reactivity with other common aquatic viruses, indicating their high specificity for MSRV detection. MIRA-FLU completed amplification within 25 min at 42°C, while MIRA-LFD achieved amplification within 12 min at 37°C. MIRA-FLU is used for real-time fluorescent quantitative detection, whereas MIRA-LFD enables visual, instrument-free reading via lateral flow test strips. In comparative analyses of clinical samples, the diagnostic results of MIRA-FLU and MIRA-LFD were compared with those of the standard conventional PCR test and showed nearly 100% consistency. Therefore, our MIRA-FLU and MIRA-LFD detection methods demonstrate excellent specificity and sensitivity, providing two simple, rapid and reliable approaches for large-scale field surveys of MSRV in resource-limited settings.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70191"},"PeriodicalIF":2.2,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147773305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rare Case of Hypermelanosis or Chromatophore Hyperplasia in Incidental Terapon jarbua (Forsskål, 1775) From a Brackish Water Aquaculture Pond. 来自咸淡水养殖池塘的偶然发生的黄颡鱼（forsskatal, 1775）的黑色素增生或染色质增生的罕见病例。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2026-04-18 DOI: 10.1111/jfd.70189

Vasanthan Koothan, Nguyen Dinh-Hung, Ayyaru Gopalakrishnan

{"title":"Rare Case of Hypermelanosis or Chromatophore Hyperplasia in Incidental Terapon jarbua (Forsskål, 1775) From a Brackish Water Aquaculture Pond.","authors":"Vasanthan Koothan, Nguyen Dinh-Hung, Ayyaru Gopalakrishnan","doi":"10.1111/jfd.70189","DOIUrl":"https://doi.org/10.1111/jfd.70189","url":null,"abstract":"Hypermelanosis associated with chromatophore hyperplasia was documented in an incidental specimen of Terapon jarbua (Forsskål, 1775) collected from a semi-intensive brackish-water culture pond used for Asian sea bass (Lates calcarifer) production. Gross examination revealed prominent, irregular, and partially coalescent hypermelanotic patches distributed along the lateral body surface and fins, accompanied by localized skin ulceration, whereas the internal organs appeared macroscopically normal. Radiographic assessment indicated that the lesions were restricted to soft tissues, with no evidence of skeletal involvement. Microscopically, the lesions were characterized by marked disruption of normal chromatophore architecture including extensive melanophore proliferation and alterations in associated chromatophore populations. Histopathological examination showed dense dermal melanin deposition, hypertrophic melanophores and disorganization in the dermal connective tissue framework. Fontana-Masson staining confirmed the presence of actively synthesized melanin within the affected tissues. Transmission electron microscopy further revealed abundant melanosomes at different stages of maturation within melanophores, together with cytoplasmic crowding and peripheral nuclear displacement. Bacteriological screening of skin lesions and internal organs on tryptic soy agar supplemented with 2% NaCl yielded no culturable bacterial growth under the incubation conditions tested. These findings are consistent with localized melanophore hyperplasia affecting the chromatophore system. This case highlights the occurrence of significant pigmentation abnormalities in a non-target species inhabiting aquaculture environments and underscores the importance of continued health surveillance and documentation of unusual phenotypic conditions in brackish-water culture systems.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70189"},"PeriodicalIF":2.2,"publicationDate":"2026-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147717000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Salt Treatment Confers Protection Against Acute Carp Edema Virus Reinfection While Promoting Viral Persistence. 盐处理可防止急性鲤鱼水肿病毒再次感染，同时促进病毒的持久性。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2026-04-15 DOI: 10.1111/jfd.70185

Mikolaj Adamek, Maria Zawisza, Justin Tze Ho Chan, Alexander Rebl, Felix Teitge, Alberto Falco, Anne-Carina Miebach, Verena Jung-Schroers, Esteban Soto, Jiří Kyslík, Dieter Steinhagen, Krzysztof Rakus, Tomas Korytar

{"title":"Salt Treatment Confers Protection Against Acute Carp Edema Virus Reinfection While Promoting Viral Persistence.","authors":"Mikolaj Adamek, Maria Zawisza, Justin Tze Ho Chan, Alexander Rebl, Felix Teitge, Alberto Falco, Anne-Carina Miebach, Verena Jung-Schroers, Esteban Soto, Jiří Kyslík, Dieter Steinhagen, Krzysztof Rakus, Tomas Korytar","doi":"10.1111/jfd.70185","DOIUrl":"10.1111/jfd.70185","url":null,"abstract":"Carp edema virus (CEV) infects the common carp (Cyprinus carpio) and causes the lethal koi sleepy disease (KSD). Signs of KSD include respiratory, detoxification, and osmoregulatory difficulties. Salt treatment re-equilibrates blood sodium levels and can save the fish. However, it is unclear whether these fish are immunized, remain chronically infected, and shed the virus, which could cause concern for aquaculture and the international fish trade. To address this issue, we examined the physiological and immunological responses following the infection of naturally immunized and naïve fish. Primary CEV infection induces inflammation in the gills and recruits granulocytic leukocyte infiltrates to the lamellae. Immunologically, the antiviral response driven by type I interferon is activated; however, both T and B lymphocytes fail to respond. As part of an immunization strategy, a primary infection followed by salt treatment effectively nullifies the pro-inflammatory response and lymphocyte immunosuppression during CEV reinfection. Our data indicate that immunization enables mechanisms such as lymphocyte activation, differentiation, antigen presentation, and an adaptive immune response and antibody production. However, immunized fish are unable to fully clear the virus for a significant period, during which they are most likely to shed infectious particles.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70185"},"PeriodicalIF":2.2,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147690468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy Assessment of Montanide GEL 02 PR and Liposome-Based Encapsulation as Adjuvants for an FrpA Subunit Vaccine Against Photobacteriosis in Solea senegalensis. Montanide GEL 02 PR和脂质体包封佐剂对塞内加尔茄光细菌病FrpA亚单位疫苗的疗效评价。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2026-04-15 DOI: 10.1111/jfd.70187

Marta A Lages, Miguel Balado, Abel M Forero, Lucía Ageitos, Jaime Rodríguez, Carlos Jiménez, Manuel L Lemos, Beatriz Magariños

{"title":"Efficacy Assessment of Montanide GEL 02 PR and Liposome-Based Encapsulation as Adjuvants for an FrpA Subunit Vaccine Against Photobacteriosis in Solea senegalensis.","authors":"Marta A Lages, Miguel Balado, Abel M Forero, Lucía Ageitos, Jaime Rodríguez, Carlos Jiménez, Manuel L Lemos, Beatriz Magariños","doi":"10.1111/jfd.70187","DOIUrl":"10.1111/jfd.70187","url":null,"abstract":"Photobacteriosis is a major threat to marine aquaculture, and the siderophore transporter FrpA represents a promising target for effective subunit vaccine development. In this study, we evaluated Montanide GEL 02 PR and phosphatidylcholine-based liposomes as aqueous and safer alternatives to Freund's adjuvant for an FrpA-based subunit vaccine in Solea senegalensis. Fish were immunized with recombinant FrpA formulated with either adjuvant, and induction of FrpA-specific IgM antibody levels was measured. Vaccine efficacy was subsequently assessed by analysis of innate immune gene expression following early pathogen exposure and by survival analysis after infection challenge. Vaccination with rFrpA-Montanide GEL 02 PR induced the highest levels of specific IgM antibodies and conferred strong protection against photobacteriosis, achieving survival rates comparable to those reported for bacterin-based vaccines. This formulation also promoted a rapid and coordinated up-regulation of immune-related genes, including transferrin (tf), lysozyme C1 (lysC1), and complement components C1q and C7. In contrast, rFrpA encapsulated in liposomes elicited weaker immune responses and limited protection. Overall, our results demonstrate that an FrpA-based subunit vaccine formulated with Montanide GEL 02 PR represents a promising strategy for controlling photobacteriosis in aquaculture.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70187"},"PeriodicalIF":2.2,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147690443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective Efficacy of Vaccines Against Grass Carp Hemorrhagic Disease: A Systematic Review and Meta-Analysis. 草鱼出血性疾病疫苗的保护效果：系统综述和meta分析。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2026-04-14 DOI: 10.1111/jfd.70188

Junhui Liu, Anfeng Liu, Yingying Wang, Jiyuan Yin, Yingying Li, Xubing Mo, Qing Wang, Shucheng Zheng

{"title":"Protective Efficacy of Vaccines Against Grass Carp Hemorrhagic Disease: A Systematic Review and Meta-Analysis.","authors":"Junhui Liu, Anfeng Liu, Yingying Wang, Jiyuan Yin, Yingying Li, Xubing Mo, Qing Wang, Shucheng Zheng","doi":"10.1111/jfd.70188","DOIUrl":"10.1111/jfd.70188","url":null,"abstract":"Grass carp hemorrhagic disease, mainly caused by grass carp reovirus (GCRV), is a major threat to grass carp aquaculture, and vaccination is considered a sustainable strategy for disease prevention and control. However, the protective efficacy of GCRV vaccines and the factors influencing it have not been systematically synthesised. In this study, literature on GCRV vaccines was retrieved from CNKI, ScienceDirect, and Web of Science in accordance with the PRISMA 2020 statement, covering database inception to March 10, 2026, followed by meta-analysis. After screening, 44 studies were included, of which 24 entered the main meta-analysis. Compared with controls, GCRV vaccines significantly reduced post-challenge mortality in grass carp (RR = 0.473, 95% CI: 0.418-0.536), with substantial between-study heterogeneity (Q = 204.61, p < 0.001; I2 = 88.8%). Subgroup analyses showed that protective efficacy varied by publication period, immunisation route, vaccine platform, and GCRV genotype background. Sensitivity analyses and leave-one-out analyses indicated overall stable results, while publication bias tests suggested possible small-study effects. Descriptive evidence mapping showed a marked increase in GCRV vaccine studies in recent years, mainly focusing on DNA vaccines and subunit/peptide vaccines, with evidence concentrated on GCRV-II. Overall, GCRV vaccines can reduce post-challenge mortality in grass carp to a certain extent, but their protective efficacy is influenced by multiple factors. Future studies should improve antigen matching, standardise evaluation systems, and develop vaccines with both efficacy and field applicability.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70188"},"PeriodicalIF":2.2,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147673922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary β-Glucan From the Marine Diatom Chaetoceros muelleri Enhances Immune Responses and Resistance of Nile Tilapia to Tilapia Lake Virus Infection. 海洋硅藻穆勒角毛藻β-葡聚糖增强尼罗罗非鱼对罗非湖病毒感染的免疫反应和抗性

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2026-04-13 DOI: 10.1111/jfd.70184

Sulaiman Madyod, Suwanna Pholmai, Thitikorn Prombanchong, Patcharapon Laksana-Aut, Sasimanas Unajak, Ikuo Hirono, Suwit Wuthisuthimethavee

{"title":"Dietary β-Glucan From the Marine Diatom Chaetoceros muelleri Enhances Immune Responses and Resistance of Nile Tilapia to Tilapia Lake Virus Infection.","authors":"Sulaiman Madyod, Suwanna Pholmai, Thitikorn Prombanchong, Patcharapon Laksana-Aut, Sasimanas Unajak, Ikuo Hirono, Suwit Wuthisuthimethavee","doi":"10.1111/jfd.70184","DOIUrl":"https://doi.org/10.1111/jfd.70184","url":null,"abstract":"This study evaluated the immunomodulatory effects of dietary β-glucan derived from the marine diatom Chaetoceros muelleri on immune responses and survival of Nile tilapia (Oreochromis niloticus) following Tilapia Lake Virus (TiLV) infection. Juvenile tilapias were fed diets supplemented with 0.1% or 0.2% β-glucan for 14 days prior to viral challenge. Fish were subsequently challenged with TiLV and immune-related gene expression was analysed in spleen and liver tissues. Fish fed the 0.1% β-glucan diet showed significantly lower cumulative mortality (26.67%) compared with the control group (55.56%) and the 0.2% group (42.22%). Dietary β-glucan significantly influenced the expression of multiple immune-related genes including cytolytic (NCCRP-1), stress-related gene (Hsp70), antimicrobial protein (C-lysozyme), cytokine (IL-8), pattern recognition receptors (TLR3, TLR5, TLR9), signalling adaptor (myD88), antiviral effector (Mx, RSAD-2), adaptive immunity (IgM, CD4). Correlation heatmap and gene co-expression network analyses revealed coordinated immune regulation, with Mx, RSAD-2 and CD4 acting as central hub genes associated with antiviral defence pathways. These results demonstrate that moderate dietary supplementation with C. muelleri-derived β-glucan enhances immune responses and improves resistance to TiLV infection in Nile tilapia, supporting its potential application as a functional feed additive in tilapia aquaculture.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70184"},"PeriodicalIF":2.2,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147673866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Lidocaine as an Adjuvant to Clove Essential Oil on Anaesthetic Induction and Recovery in Nile Tilapia. 丁香精油辅助利多卡因对尼罗罗非鱼麻醉诱导和恢复的影响。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2026-04-12 DOI: 10.1111/jfd.70186

J A Franco-Ortega, P J Albertos-Alpuche, P Mora-Medina, R Martínez-Yáñez

{"title":"Effect of Lidocaine as an Adjuvant to Clove Essential Oil on Anaesthetic Induction and Recovery in Nile Tilapia.","authors":"J A Franco-Ortega, P J Albertos-Alpuche, P Mora-Medina, R Martínez-Yáñez","doi":"10.1111/jfd.70186","DOIUrl":"https://doi.org/10.1111/jfd.70186","url":null,"abstract":"Immersion anaesthesia is widely used in fish handling and research, yet optimization of protocols requires not only effective immobilization but also consideration of internal physiological responses. This study evaluated the effects of immersion-administered lidocaine (60 and 80 mg/L) as an adjuvant to clove essential oil (CEO; 100 μL/L) on anaesthesia induction and recovery in juvenile Oreochromis niloticus under tightly controlled temperature (27.5°C ± 0.3°C). Behavioural endpoints included loss of equilibrium (LOE), time to an operational anaesthetic plane and recovery time; opercular respiratory rate was assessed as an internal physiological indicator. In the main experiment, adding lidocaine to CEO did not significantly alter LOE, time to anaesthetic plane or recovery time relative to CEO alone. In contrast, respiratory-rate trajectories differed significantly among treatments during both induction and recovery, indicating protocol-dependent modulation of ventilatory dynamics despite similar time-based anaesthetic endpoints. A complementary preliminary experiment showed that lidocaine alone (60 or 80 mg/L) did not induce LOE or anaesthetic plane within a predefined 5-min exposure window, although it produced clear dose-dependent changes in respiratory rate relative to water and ethanol controls. Together with qualitative observations of reduced behavioural activation, these findings are compatible with a possible calming or sedation-like effect of lidocaine, although this was not formally measured and should be considered a hypothesis. Overall, lidocaine did not improve the operational speed of a CEO-based immersion anaesthetic protocol in Nile tilapia, but it did modulate physiological responses relevant to protocol refinement. These results highlight the importance of integrating both behavioural and internal physiological indicators, together with strict thermal control, when evaluating immersion anaesthesia protocols in fish.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70186"},"PeriodicalIF":2.2,"publicationDate":"2026-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147662848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0