Journal of fish diseases最新文献_第2页

Rapid Detection of Aeromonas veronii Using GGDEF Gene-Based Colorimetric Loop-Mediated Isothermal Amplification.

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-03-11 DOI: 10.1111/jfd.14112

Tien Tien Vicky Lau, Suat Moi Puah, Kek Heng Chua

{"title":"Rapid Detection of Aeromonas veronii Using GGDEF Gene-Based Colorimetric Loop-Mediated Isothermal Amplification.","authors":"Tien Tien Vicky Lau, Suat Moi Puah, Kek Heng Chua","doi":"10.1111/jfd.14112","DOIUrl":"https://doi.org/10.1111/jfd.14112","url":null,"abstract":"Aeromonas veronii has emerged as an important fish pathogen that poses threats to the aquaculture industry worldwide, and rapid detection is essential to minimise negative economic impact. In this study, a colorimetric loop-mediated isothermal amplification (LAMP) assay was developed to rapidly detect A. veronii, targeting a gene encoding GGDEF domain-containing diguanylate cyclase. The entire LAMP reaction could be completed in 30 min at 65°C based on colour change from pink to yellow. In addition, the assay demonstrated 100% specificity with no cross reaction with other common fish pathogens. The detection limit (LOD) was 500 pg/μL using purified plasmid and 1.72 × 105 cfu/mL (equivalent to 1.72 × 102 cfu/reaction) using crude genomic DNA extracted from the pure culture of A. veronii ATCC 9071. LAMP demonstrated comparable performance to conventional PCR using 57 bacterial isolates, with 100% (20/20) specificity, 91.9% (34/37) sensitivity, 94.7% (54/57) accuracy and a 0.888 Cohen's kappa value. Lastly, the LOD of LAMP in a spiked water sample was 1.72 × 105 cfu/mL (equivalent to 3.44 × 103 cfu/reaction). Overall, our LAMP assay has a high level of diagnostic agreement with conventional PCR and can be used as a valuable tool for the rapid detection of A. veronii from environmental samples.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14112"},"PeriodicalIF":2.2,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of Biomarkers in Plasma and Faeces to Evaluate Physiological Status of Atlantic Salmon (Salmo salar L.) During Salmonid Alphavirus Infection.

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-03-05 DOI: 10.1111/jfd.14114

Eirik Hoel, Frederike Keitel-Gröner, Christina Aspaas Husebø, David Lausten Knudsen, Julia Mullins, Christian Wallace, Hanne Log Persson, Hanna S Bjerke-Ertenstein, Maren Iden Bjerkeset, Kjetil Berge

{"title":"Use of Biomarkers in Plasma and Faeces to Evaluate Physiological Status of Atlantic Salmon (Salmo salar L.) During Salmonid Alphavirus Infection.","authors":"Eirik Hoel, Frederike Keitel-Gröner, Christina Aspaas Husebø, David Lausten Knudsen, Julia Mullins, Christian Wallace, Hanne Log Persson, Hanna S Bjerke-Ertenstein, Maren Iden Bjerkeset, Kjetil Berge","doi":"10.1111/jfd.14114","DOIUrl":"https://doi.org/10.1111/jfd.14114","url":null,"abstract":"Salmonid alphavirus (SAV) is the causative agent of pancreas disease (PD), a disease that can cause severe implications for marine farming of Atlantic salmon. This study examines physiological changes in Atlantic salmon during SAV infection through a controlled trial and two field trials. In the controlled trial, plasma creatine kinase (CK), alanine aminotransferase (ALAT) and aspartate aminotransferase (ASAT) levels increased significantly 4 weeks post challenge, peaked at 8 weeks and by 12 weeks, ALAT levels returned to pre-challenge levels, while CK and ASAT remained elevated. Weekly mortality peaked at 4.1% in week 5, and there was an 89% reduction in appetite in week 4 post challenge. In two field trials in southern Norway, SAV3 was detected in spring/early summer by reverse transcription quantitative polymerase chain reaction (RT-qPCR). As SAV prevalence increased, plasma CK, ALAT, ASAT and lactate dehydrogenase (LDH) levels peaked, along with the stress markers cortisol and tetrahydrocortisone in faeces. Despite low mortality (< 0.2% weekly), a decrease in appetite was observed in both field trials. Understanding the physiological status of the fish through monitoring tools like RT-qPCR, blood chemistry and stress markers provides a foundation for implementing mitigation measures such as stress reduction, timed harvest or clinical nutrition at the onset of pancreas disease.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14114"},"PeriodicalIF":2.2,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and Pathogenicity of Lactococcus Species in Nile Tilapia (Oreochromis niloticus) and Asian Sea Bass (Lates calcarifer).

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-03-04 DOI: 10.1111/jfd.14113

Janchai Wongkaew, Satid Chatchaiphan, Suwimon Taengphu, Ha Thanh Dong, Saengchan Senapin, Sanit Piyapattanakorn

{"title":"Identification and Pathogenicity of Lactococcus Species in Nile Tilapia (Oreochromis niloticus) and Asian Sea Bass (Lates calcarifer).","authors":"Janchai Wongkaew, Satid Chatchaiphan, Suwimon Taengphu, Ha Thanh Dong, Saengchan Senapin, Sanit Piyapattanakorn","doi":"10.1111/jfd.14113","DOIUrl":"https://doi.org/10.1111/jfd.14113","url":null,"abstract":"Lactococcosis, primarily caused by Lactococcus garvieae, is prevalent in various freshwater fish species globally. Yet, its significance in Thai fish aquaculture remains poorly understood. This study investigated Lactococcosis-associated diseases across four red tilapia (Oreochromis sp.) and one Asian sea bass (Lates calcarifer) farm in Thailand in 2022. Chronically diseased fish displaying clinical signs such as exophthalmia or corneal opacity, erratic swimming, scale loss and skin haemorrhage, as well as apparently healthy specimens, were studied. Bacterial isolation was performed from the fish internal organs. Gram-positive bacterial isolates were selected and later identified as L. lactis (n = 4), L. taiwanensis (n = 1), L. garvieae (n = 2) and L. petauri (n = 2) based on bacterial 16S rDNA and gyrB partial sequences. Sub-adults of Nile tilapia were intracoelomic (IC) injected with representatives of each of the three Lactococcus species (L. garvieae, L. lactis and L. taiwanensis) at varying doses (103-107 CFU/fish), while juvenile Asian sea bass were IC injected with L. petauri at doses ranging from 102 to 108 CFU/fish. Control groups received culture medium alone. By the end of the 21-day test, mortality rates from all challenge groups ranged from 0% to 14.82%. Some clinical signs were observed in groups challenged with higher doses of the bacteria. Enzyme-linked immunosorbent assay (ELISA) showed that survivor fish exposed to high bacterial doses elicited specific antibodies IgM. In summary, this study identifies the presence of Lactococcus species in farmed fishes, which exhibit low virulence in Nile tilapia and Asian sea bass under laboratory-controlled challenges. These bacterial isolates appear to be opportunistic rather than pathogenic, potentially indicating underlying health challenges within the affected farms.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14113"},"PeriodicalIF":2.2,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First Investigation of Trichodinid Species (Ciliophora: Trichodinidae) on Farmed Hybrid Yellow Catfish (Tachysurus fulvidraco × Tachysurus vachelli) in China.

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-03-03 DOI: 10.1111/jfd.14100

Enze Zhang, Hao Yang, Xiao Tu, Zemao Gu

{"title":"First Investigation of Trichodinid Species (Ciliophora: Trichodinidae) on Farmed Hybrid Yellow Catfish (Tachysurus fulvidraco × Tachysurus vachelli) in China.","authors":"Enze Zhang, Hao Yang, Xiao Tu, Zemao Gu","doi":"10.1111/jfd.14100","DOIUrl":"https://doi.org/10.1111/jfd.14100","url":null,"abstract":"Hybrid yellow catfish (Tachysurus fulvidraco♀ × Tachysurus vachelli♂) is a significant aquaculture variety in China. It is easy to be infected by trichodinids, causing serious economic losses. However, the species investigation of trichodinids on hybrid yellow catfish is relatively deficient, which seriously hinders the prevention and control of trichodiniasis. Hence, trichodinids were collected in a hybrid yellow catfish farm in Hubei Province from June 2022 to October 2023 and identified by morphological and molecular biology methods. A new species Tripartiella luhuensis sp. nov. and nine Trichodina species were described. Phylogenetic analyses demonstrated that T. luhuensis nested in the Trichodinella-Tripartiella-Paratrichodina clade, indicating that the genus Tripartiella is not a monophyletic group. Besides, Trichodinid species from Siluriformes hosts have relatively similar evolutionary positions. Histopathological analysis revealed that the parasitism of trichodinids caused mechanical damage and mucus hypersecretion to gill of the host. The present study reports trichodinid species on hybrid yellow catfish for the first time and adds one new species to the genus Tripartiella. We also indicate the importance of preventing and controlling trichodiniasis in hybrid yellow catfish farming.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14100"},"PeriodicalIF":2.2,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immune Response of Nile Tilapia (Oreochromis niloticus) Vaccinated With Diatom-Based Oral Vaccines Against Piscine Francisellosis. 用硅藻口服疫苗接种尼罗罗非鱼（Oreochromis niloticus）对鱼弗氏杆菌病的免疫反应。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-03-01 DOI: 10.1111/jfd.14111

Collin Meyer, Roshan P Shrestha, Ruth Milston-Clements, Sarah Gibson, Taylor I Heckman, Zeinab Yazdi, Esteban Soto

{"title":"Immune Response of Nile Tilapia (Oreochromis niloticus) Vaccinated With Diatom-Based Oral Vaccines Against Piscine Francisellosis.","authors":"Collin Meyer, Roshan P Shrestha, Ruth Milston-Clements, Sarah Gibson, Taylor I Heckman, Zeinab Yazdi, Esteban Soto","doi":"10.1111/jfd.14111","DOIUrl":"https://doi.org/10.1111/jfd.14111","url":null,"abstract":"Piscine francisellosis is a highly infectious and economically significant disease caused by Francisella orientalis in tilapia (Oreochromis spp.). There are currently no approved treatments or commercial vaccines for this disease in cultured fish. Injectable vaccines using diatoms as antigen expression vectors have demonstrated efficacy in tilapia models; however, no oral vaccine trials have been performed. We hypothesised that fusion proteins consisting of F. orientalis IglC and flagellin expressed in Thalassiosira pseudonana diatoms will act as self-adjuvanting antigen delivery systems to confer a protective immune response against F. orientalis in tilapia when administered as top-coated feed. Different treatments were immunised and subsequently provided with one or two boosters prior to challenge. Fish were challenged with virulent F. orientalis via immersion thirty days post initial immunisation. Tilapia immune response was assessed 24 h post-challenge by quantifying il-12, il-10, ifn-γ and tgf-β gene expression in gills and internal organs. Morbidity and mortality were monitored for 21 days after challenge and bacterial load was assessed in survivors. Findings indicate significant changes in the expression of ifn-γ and tgf-β in immunised fish, but similar mortality rates and bacterial load across all exposed groups.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14111"},"PeriodicalIF":2.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143530945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and Efficacy of a Chitosan Nanoparticle-Based Immersion Vaccine Targeting Segment 4 of Tilapia Lake Virus.

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-02-28 DOI: 10.1111/jfd.14106

Chanasorn Thanapasuk, Puntanat Tattiyapong, Jidapa Yamkasem, Sirikorn Kitiyodom, Piyathip Setthawong, Tuchakorn Lertwanakarn, Win Surachetpong

{"title":"Development and Efficacy of a Chitosan Nanoparticle-Based Immersion Vaccine Targeting Segment 4 of Tilapia Lake Virus.","authors":"Chanasorn Thanapasuk, Puntanat Tattiyapong, Jidapa Yamkasem, Sirikorn Kitiyodom, Piyathip Setthawong, Tuchakorn Lertwanakarn, Win Surachetpong","doi":"10.1111/jfd.14106","DOIUrl":"https://doi.org/10.1111/jfd.14106","url":null,"abstract":"Tilapia lake virus disease (TiLVD), which is caused by tilapia lake virus (TiLV), has resulted in significant damage to global tilapia farming. TiLV is a negative-sense single-strand RNA virus consisting of 10 genome segments. To date, no commercial vaccine against TiLVD has been developed, and effective strategies to control and prevent TiLVD are lacking. In this study, we developed and tested a chitosan nanoparticle-based immersion recombinant protein targeting segment 4 (S4) of TiLV under both laboratory and field conditions. The open reading frame of S4 of TiLV was cloned into pET28a (+) and expressed by Escherichia coli BL21(DE3). The size of the nanoTiLV-S4 (CNS4) vaccine was 284 ± 9.2 nm, which is smaller than the pre-nanoencapsulation vaccine size of 2268 ± 41.8 nm. Transmission electron microscopy revealed that the nanoS4 particles had a round shape, uniform appearance and positive zeta potential of 17.7 ± 0.7 mV. Further analysis showed that the nanoS4 antigen was deposited on the fish gills and intestines and taken up into the epithelial cells within 30 min of immersion. Under laboratory infection using a cohabitation challenge model, the CNS4 vaccine demonstrated a relative percent survival (RPS) of 25%. In field conditions, the vaccine showed an RPS of 31.88% compared to the unvaccinated group. Overall, our study demonstrates that the new nanoTiLV-S4 vaccine can be absorbed by the fish epithelium and reduces mortality caused by TiLV. However, further optimisation and field trials are necessary to improve the efficacy of the CNS4 vaccine and to test it under various farm conditions.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14106"},"PeriodicalIF":2.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural Infection of Two Gourami Species (Trichopodus spp.) With Infectious Spleen and Kidney Necrosis Virus (ISKNV): Clinical, Molecular and Histopathological Findings.

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-02-27 DOI: 10.1111/jfd.14110

Nguyen Dinh-Hung, Ha Thanh Dong, Kornsunee Phiwsaiya, Suwimon Taengphu, Nguyen Vu Linh, Satid Chatchaiphan, Channarong Rodkhum, Hung N Mai, Arun K Dhar, Saengchan Senapin

{"title":"Natural Infection of Two Gourami Species (Trichopodus spp.) With Infectious Spleen and Kidney Necrosis Virus (ISKNV): Clinical, Molecular and Histopathological Findings.","authors":"Nguyen Dinh-Hung, Ha Thanh Dong, Kornsunee Phiwsaiya, Suwimon Taengphu, Nguyen Vu Linh, Satid Chatchaiphan, Channarong Rodkhum, Hung N Mai, Arun K Dhar, Saengchan Senapin","doi":"10.1111/jfd.14110","DOIUrl":"https://doi.org/10.1111/jfd.14110","url":null,"abstract":"Infectious spleen and kidney necrosis virus (ISKNV) has a broad host range and poses a significant threat to aquaculture species. Herein, we report two disease outbreaks in snakeskin gourami (Trichopodus pectoralis) in Thailand, marked by skin haemorrhage, scale loss, internal organ discoloration and degeneration and a mortality exceeding 60%. Additionally, three spot gourami (Trichopodus trichopterus) cultured in the same ponds along with snakeskin gourami were found to be affected and tested positive for the virus. Histopathological examination revealed extensive necrosis of haematopoietic tissues in the kidneys and spleen, accompanied by the presence of numerous megalocytic cells in both fish species. Real-time PCR assays, using protocols targeting the major capsid protein (MCP) gene in a broad range of Megalocytivirus genotypes, confirmed the presence of ISKNV in the infected fish. Furthermore, molecular and phylogenetic analyses based on the MCP gene (1362 bp) and the adenosine triphosphatase (ATPase) gene (720 bp) revealed that the ISKNV strains in gourami and other fish hosts were closely related, suggesting possible cross-species transmission. This report extends the host range of ISKNV and highlights the need to prevent the spread of this virus across species boundaries. Further research is needed to understand the pathogenicity and transmission routes of the virus, gain insights into its epidemiology and develop strategies to mitigate disease outbreaks.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14110"},"PeriodicalIF":2.2,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental Challenge of Chinook Salmon (Oncorhynchus tshawytscha) With Tenacibaculum maritimum and Tenacibaculum dicentrarchi Fulfils Koch's Postulates.

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-02-26 DOI: 10.1111/jfd.14088

Karthiga Kumanan, Jeremy Carson, Ryan B J Hunter, Anne Rolton, Ulla von Ammon, Chaya Bandaranayake, Connie Angelucci, Richard N Morrison, Seumas P Walker, Jane E Symonds, Kate S Hutson

{"title":"Experimental Challenge of Chinook Salmon (Oncorhynchus tshawytscha) With Tenacibaculum maritimum and Tenacibaculum dicentrarchi Fulfils Koch's Postulates.","authors":"Karthiga Kumanan, Jeremy Carson, Ryan B J Hunter, Anne Rolton, Ulla von Ammon, Chaya Bandaranayake, Connie Angelucci, Richard N Morrison, Seumas P Walker, Jane E Symonds, Kate S Hutson","doi":"10.1111/jfd.14088","DOIUrl":"https://doi.org/10.1111/jfd.14088","url":null,"abstract":"The bacterial skin disease tenacibaculosis, caused by Tenacibaculum species, affects numerous economically important marine fish, including salmonids. This study reports the ability of three Tenacibaculum maritimum strains, belonging to different molecular O-AGC types, and a single Tenacibaculum dicentrarchi strain to induce tenacibaculosis in farmed Chinook salmon (Oncorhynchus tshawytscha, Walbaum 1792) in Aotearoa New Zealand. Naïve Chinook salmon were exposed to T. maritimum (2 × 108 cells/mL) and T. dicentrarchi (2 × 107 cells/mL) by immersion using natural seawater. Clinical signs of tenacibaculosis were apparent in all T. maritimum strains used in the challenged fish. Of these, 100% of the fish challenged with O-AGC Type 2-1 and Type 3-2 strains became moribund, whereas only 60% of the O-AGC Type 3-0 challenged fish became moribund. Fish exposed to T. dicentrarchi showed more severe symptoms, exposing musculature in 51% of the challenged population, with 28% of fish becoming moribund. Gross pathological signs of fin rot, scale loss, skin ulcers and haemorrhagic skin spots were observed for both Tenacibaculum species and were consistent with those observed on farmed fish. Pure T. maritimum and T. dicentrarchi cultures were reisolated from epidermal damage of challenged fish. Tenacibaculum species was not isolated from the anterior kidney of affected fish, which indicates no systemic infection in Chinook salmon.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14088"},"PeriodicalIF":2.2,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143501964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histological Qualitative Assessment of Tissue Lesions in Atlantic Salmon (Salmo salar L.) Following Caudal Vasculature Blood Sampling.

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-02-25 DOI: 10.1111/jfd.14098

A S Dalum, H Romstad, L Boissonnot, S Stensby-Skjærvik, C Klykken

引用次数: 0

Discovery of Potential Anthelmintic Agents Against Gyrodactylus kobayashii Through Computer-Aided Drug Design and In Vivo Evaluation.

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-02-24 DOI: 10.1111/jfd.14102

Jing Dong, Liwei Xia, Yongtao Liu, Qiuhong Yang, Ning Xu, Xiaohui Ai, Shun Zhou

{"title":"Discovery of Potential Anthelmintic Agents Against Gyrodactylus kobayashii Through Computer-Aided Drug Design and In Vivo Evaluation.","authors":"Jing Dong, Liwei Xia, Yongtao Liu, Qiuhong Yang, Ning Xu, Xiaohui Ai, Shun Zhou","doi":"10.1111/jfd.14102","DOIUrl":"https://doi.org/10.1111/jfd.14102","url":null,"abstract":"Monogeneans are ectoparasitic flatworms causing significant economic losses in aquaculture. This study aimed to identify potential anthelmintic agents against these parasites by integrating computer-aided drug design (CADD) and in vivo evaluation. The β-tubulin gene, a well-established anthelmintic target, was cloned from Gyrodactylus kobayashii and its three-dimensional structure was generated using homology modelling. Virtual screening of 2319 FDA-approved drugs and nine common benzimidazoles against the modelled β-tubulin identified several promising compounds with low binding energy. Subsequent in vivo anthelmintic efficacy and acute toxicity assays in goldfish revealed etravirine as a potent candidate with an EC50 value of 0.55 mg/L and a therapeutic index (TI) greater than 18.18. This favourable safety profile highlights etravirine's potential for controlling monogenean infections in aquaculture. Flubendazole and mebendazole also demonstrated potent anthelmintic activity, with EC50 values of 0.022 and 0.023 mg/L and therapeutic indices exceeding 45.45 and 43.48, respectively. Molecular dynamics simulations confirmed stable binding modes for flubendazole and mebendazole with β-tubulin, providing mechanistic insights into their anthelmintic activity. Overall, this study demonstrated the utility of CADD in identifying potential therapeutic agents against monogenean and underscored the importance of β-tubulin as a key target for anthelmintic therapy, contributing to the development of sustainable aquaculture practices.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14102"},"PeriodicalIF":2.2,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0