Journal of fish diseases最新文献_第2页

Isolation and Genomic Characterisation of Lymphocystivirus platichthys1 in Brackish-Water Cultured Spotted Seabass Lateolabrax maculatus in Zhuhai, South China. 珠海市淡咸水养殖斑点海鲈淋巴囊病毒platichthys1的分离及基因组特征分析

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-05-14 DOI: 10.1111/jfd.14139

Hongling Ma, Changhong Cheng, Yiqin Deng, Zhixun Guo, Yong Li, Guangxin Liu, Huajian Lin, Liwen Xu, Juan Feng

{"title":"Isolation and Genomic Characterisation of Lymphocystivirus platichthys1 in Brackish-Water Cultured Spotted Seabass Lateolabrax maculatus in Zhuhai, South China.","authors":"Hongling Ma, Changhong Cheng, Yiqin Deng, Zhixun Guo, Yong Li, Guangxin Liu, Huajian Lin, Liwen Xu, Juan Feng","doi":"10.1111/jfd.14139","DOIUrl":"https://doi.org/10.1111/jfd.14139","url":null,"abstract":"Lymphocystis disease virus (LCDV) stands as one of the most widespread causative agents affecting a diverse array of teleost globally. According to the literature, LCDV encompasses four species, and Lymphocystivirus paralichthys1 (known as LCDV-C) has long been regarded as the predominant national epidemic isolate in mainland China. In this study, two instances of severe mass mortalities associated with LCDV in brackish-water cultured juvenile spotted seabass in Zhuhai, South China, were monitored starting from early April 2023. The death toll in these two cases peaked at approximately 4000 and 10,000 fish per day, respectively, with cumulative mortalities reaching around 70% within 19 days of the disease outbreak. Based on clinical symptoms, histopathological analyses and transmission electron microscopy observations, the diseases were diagnosed as LCDV infections. The virus was isolated using the Mandarin fish fry (MFF-1) cell line and named LCDV-ZH04/23. Subsequently, LCDV-ZH04/23 was roughly purified, and its entire genome was sequenced and annotated. The results indicated that ZH04/23 consists of 102,661 base pairs, encodes 108 putative viral genes and has a G/C content of 29.3%. Genomic collinearity analysis revealed that the whole genome of ZH04/23 exhibits the highest similarity (99.9%) to the well-known Lymphocystivirus platichthys1 (LCDV-1) from Europe, but shows significant divergence from the other three LCDV species. Collectively, this study marks the first isolation and characterisation of L. platichthys1 at the whole genome level from cases of severe mass mortalities in pond-cultured spotted seabass in China. Moreover, it is the first to identify L. platichthys1 as an emerging pathogen in cultured spotted seabass.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14139"},"PeriodicalIF":2.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143974273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immune Response and Protective Efficacy of β-Glucan and Alkoxyglycerol as Adjuvant in Streptococcus agalactiae Formalin-Inactivated Vaccine in Nile Tilapia (Oreochromis niloticus). β-葡聚糖和烷氧基甘油作为无乳链球菌福尔马林灭活疫苗佐剂对尼罗罗非鱼的免疫应答及保护作用

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-05-14 DOI: 10.1111/jfd.14141

Ritam Guha, K Nandhakumar, Omkar Vijay Byadgi, Shih-Chu Chen, Preetham Elumalai

{"title":"Immune Response and Protective Efficacy of β-Glucan and Alkoxyglycerol as Adjuvant in Streptococcus agalactiae Formalin-Inactivated Vaccine in Nile Tilapia (Oreochromis niloticus).","authors":"Ritam Guha, K Nandhakumar, Omkar Vijay Byadgi, Shih-Chu Chen, Preetham Elumalai","doi":"10.1111/jfd.14141","DOIUrl":"https://doi.org/10.1111/jfd.14141","url":null,"abstract":"Nile tilapia (Oreochromis niloticus) farms face streptococcus agalactiae infections causing mass mortality following economic losses. To date, no commercial fish vaccine is available in India to protect against streptococcosis due to a lack of proper adjuvants and vaccine targets. Multiple studies justify that immunomodulators like β-glucan and Alkoxy glycerol (AKG) have improved immunity in fish. β-glucan and AKG are natural homopolymers and lipids, respectively, which have proven immunostimulant capabilities in fish and can be effectively used as a prominent adjuvant to improve vaccine efficacy. The present study aims to demonstrate the improved adjuvant efficacy of the formalin-inactivated S. agalactiae vaccine. Nile tilapia fingerlings were immunised with formalin-inactivated S. agalactiae vaccines mixed with β-glucan and AKG. Each week postvaccination, the serum nonspecific responses were measured by lysozyme, myeloperoxidase, SOD and bactericidal activity. Following the challenge after 28 days postvaccination, the survival rate achieved was 32.14%, 57.14%, 53.57% and 71.43% in the respective experimental groups. The immune gene expressions of IgM, TCRβ and MHCII were evoked postvaccination. The specific serum IgM titre values were significantly upregulated in 14- and 28-day postvaccinated experimental groups. The study highlights the enhanced activity of β-glucan and AKG incorporated vaccine, which has the potency towards commercialisation for sustainable aquaculture.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14141"},"PeriodicalIF":2.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of Diagnostic Tools to Detect Acute Gas Bubble Disease in Atlantic Salmon (Salmo salar L.). 大西洋鲑鱼急性气泡病诊断工具的评估

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-05-13 DOI: 10.1111/jfd.14142

S Stensby-Skjærvik, L Boissonnot, A S Dalum, T Storsul, O K Hess-Erga, S Calabrese, K Segadal, L Andersen, H Wahl-Ovesen, I Rønnestad

{"title":"Assessment of Diagnostic Tools to Detect Acute Gas Bubble Disease in Atlantic Salmon (Salmo salar L.).","authors":"S Stensby-Skjærvik, L Boissonnot, A S Dalum, T Storsul, O K Hess-Erga, S Calabrese, K Segadal, L Andersen, H Wahl-Ovesen, I Rønnestad","doi":"10.1111/jfd.14142","DOIUrl":"https://doi.org/10.1111/jfd.14142","url":null,"abstract":"Currently, diagnosis of gas bubble disease (GBD) in fish relies on visual observations of bubbles in various body parts and exophthalmus. These observations lack quantified information about gas bubble load, limiting their diagnostic value. This study evaluated the suitability of existing and novel diagnostic tools for detecting GBD in Atlantic salmon exposed to supersaturation induced by pressure reduction. Existing methods were complemented with semi-quantitative scoring models of gas bubbles or tissue damages and tested for their potential diagnostic value. Additionally, methods commonly used for diagnosis of GBD in humans, such as detection of gas bubbles in blood using ultrasound, angiotensin converting enzyme (ACE) concentration and haematological parameters, were tested for their suitability for salmon. This study demonstrated that semi-quantification of gas bubbles using ultrasound was the most reliable method to evaluate the risk of developing acute GBD and to determine whether it is the cause of mortality. The findings suggest that the other assessed methods are unreliable for detecting acute GBD in fish.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14142"},"PeriodicalIF":2.2,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytotoxicity and Antiparasitic Effects of Toltrazuril Against Enteromyxum leei in Olive Flounders: In Vitro and In Vivo Analyses. 托曲祖利对橄榄比目鱼leei肠杆菌的细胞毒性和抗寄生作用：体外和体内分析。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-05-12 DOI: 10.1111/jfd.14125

Young Juhn Lee, Yeong Eun Oh, Eung Jun Lee, Lyu Jin Jun, Joon Bum Jeong

{"title":"Cytotoxicity and Antiparasitic Effects of Toltrazuril Against Enteromyxum leei in Olive Flounders: In Vitro and In Vivo Analyses.","authors":"Young Juhn Lee, Yeong Eun Oh, Eung Jun Lee, Lyu Jin Jun, Joon Bum Jeong","doi":"10.1111/jfd.14125","DOIUrl":"https://doi.org/10.1111/jfd.14125","url":null,"abstract":"The parasite Enteromyxum leei causes serious emaciation disease in marine fish species. Toltrazuril exhibits antiparasitic effects; however, the simultaneous impacts of toltrazuril on parasites and hosts are unclear. Here, we evaluate the cytotoxicity of three commercial toltrazuril products (A, B and C) and the treatment efficacy against E. leei infections in olive flounder (Paralichthys olivaceus). Product A was confirmed safe in Hirame natural embryo cells, even at a toltrazuril concentration of 50 μg/mL, and was selected for further studies. In vitro assays revealed that toltrazuril effectively killed E. leei spores at concentrations of 5.0, 7.5 and 10.0 μg/mL. In vivo experiments on olive flounders infected with E. leei showed that oral toltrazuril administration at doses of 10 and 20 mg/kg body weight for 5 days effectively damaged spores in the fish intestine, with E. leei death confirmed by propidium iodide staining. Administering toltrazuril mixed feed (20 mg/kg) to infected olive flounders in a fish-farm setting also proved effective in killing spores present in the intestine. Furthermore, Hoechst-propidium iodide double staining confirmed that toltrazuril was effective in killing E. leei spores both in vitro and in vivo, indicating its potential as a treatment for emaciation diseases in marine fish.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14125"},"PeriodicalIF":2.2,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144013320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Behaviour and Dispersal of Mobile Salmon Lice When Detached From the Host. 移动鲑鱼虱体与寄主分离后的行为和扩散。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-05-10 DOI: 10.1111/jfd.14143

Luke T Barrett, Mari F Jensen, Sussie Dalvin, Frode Oppedal

{"title":"Behaviour and Dispersal of Mobile Salmon Lice When Detached From the Host.","authors":"Luke T Barrett, Mari F Jensen, Sussie Dalvin, Frode Oppedal","doi":"10.1111/jfd.14143","DOIUrl":"https://doi.org/10.1111/jfd.14143","url":null,"abstract":"Sea lice can flourish when salmon are farmed in open sea-cages, necessitating treatments to control outbreaks and reduce larval export. However, mobile ectoparasitic stages can be dislodged during crowding or other procedures, and potentially reinfest farmed or wild fish. We studied vertical movements and host-finding behaviours of mobile salmon lice (Lepeophtheirus salmonis) released into the water column, and used those data to parameterise a biophysical dispersal model. Over 0-0.4 m in tanks, larger stages sank more quickly than smaller stages (1.5 cf. 0.6 cm s-1), with sinking speeds of adult lice validated in deeper tanks (0-3 m) and a fjord (~15-24 m). Adult males had the greatest behavioural component, sinking more slowly with host cues present and faster when dead. Detached lice were able to intercept new hosts in a tank (23% within 5 min). We also investigated Caligus elongatus, but found their strong swimming not amenable to study. A hydrodynamic dispersal model indicated that detached lice can reach neighbouring cages but rarely neighbouring farms before sinking below cage depth. Simulations comparing farm sites highlighted the influence of site-specific current conditions on dispersal kernels, and indicated that crowding/handling fish during favourable tides can reduce downstream risk.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14143"},"PeriodicalIF":2.2,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence Estimates of Anisakis spp. Infection in Fish From Indonesia: A Systematic Review and Meta-Analysis. 印度尼西亚鱼类中异尖线虫感染率的估计：系统回顾和荟萃分析。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-05-09 DOI: 10.1111/jfd.14140

Handang Widantara, Dian Meididewi Nuraini, Ekky Ilham Romadhona, Sutanti Sutanti, Morsid Andityas

{"title":"Prevalence Estimates of Anisakis spp. Infection in Fish From Indonesia: A Systematic Review and Meta-Analysis.","authors":"Handang Widantara, Dian Meididewi Nuraini, Ekky Ilham Romadhona, Sutanti Sutanti, Morsid Andityas","doi":"10.1111/jfd.14140","DOIUrl":"https://doi.org/10.1111/jfd.14140","url":null,"abstract":"Anisakis spp. is a notable fish parasite linked to foodborne diseases, representing a significant public health concern in Indonesia. Despite various reports from different regions indicating fluctuating prevalence levels of Anisakis spp. in diverse fish species in Indonesia, the overarching national prevalence remains uncertain. This study aimed to estimate the overall prevalence of Anisakis spp. infection among Indonesian fish. A comprehensive literature search was conducted across six online databases, and articles were screened based on predefined inclusion criteria. A total of 65 studies fit the inclusion criteria with an average quality assessment score of 7.23 ± 1.77. The overall pooled prevalence of Anisakis spp. infection in fish in Indonesia was 32.63% (95% CI, 25.44%-40.73%). Subgroup analyses conducted based on province, sample origin, ecological niche and fish genus revealed no statistically significant differences. Meta-regression indicated no significant changes in infection rates over time (p-value: 0.44). The study also confirmed the absence of publication bias. In conclusion, the overall prevalence of Anisakis spp. infection is relatively high and poses a potential health risk related to fish consumption, warranting further epidemiological research into its impact on human health.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14140"},"PeriodicalIF":2.2,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143968789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diplostomum-Induced Sac Formation in Lenses of Ameiurus Bullheads: A Host Defence Response? 双瓣气孔诱导的牛头虾晶状体囊形成：宿主防御反应？

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-05-07 DOI: 10.1111/jfd.14137

Markéta Ondračková, Andrea Valigurová, Iveta Hodová, Veronika Bartáková, Maria Yu Tkachenko, Michal Janáč, Lukáš Vetešník

{"title":"Diplostomum-Induced Sac Formation in Lenses of Ameiurus Bullheads: A Host Defence Response?","authors":"Markéta Ondračková, Andrea Valigurová, Iveta Hodová, Veronika Bartáková, Maria Yu Tkachenko, Michal Janáč, Lukáš Vetešník","doi":"10.1111/jfd.14137","DOIUrl":"https://doi.org/10.1111/jfd.14137","url":null,"abstract":"Trematodes of the genus Diplostomum (Diplostomidae) are widely distributed and significant fish pathogens known for causing a range of negative effects. Any mechanism that protects the host from the parasite thus represents an evolutionary advantage. In this study, we observed an unusual localisation of Diplostomum metacercariae, which were displaced into the sac protruding from the lens, in North-American bullhead catfish species of the genus Ameiurus. The occurrence of the sac was found in all sampling sites (Czech Republic) where A. nebulosus and A. melas were infected with Diplostomum, regardless of the parasite species, as both D. pseudospathaceum and D. spathaceum induced sac formation. The probability of sac formation increased with the intensity of Diplostomum infection. Experimental infection of juvenile A. melas showed that the first signs of sac formation appeared shortly after the infection. While visual observation under the light microscope suggested the first signs at 7 days post-infection, histological examination documented the start of epithelial proliferation by the third day post-infection. Our study suggests that the process of sac formation, along with parasite displacement, might present a host reaction to mitigate the negative effects of infection and the impaired vision caused by the parasite.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14137"},"PeriodicalIF":2.2,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144020230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First Report of Philometroides sanguineus (Nematoda: Philometridae) in Farmed Goldfish (Carassius auratus) in Italy. 意大利养殖金鱼（Carassius auratus）中血丝缕蝇（线虫纲：血丝缕蝇科）首次报道。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-04-27 DOI: 10.1111/jfd.14138

Teresa Pirollo, Perla Tedesco, Marialetizia Fioravanti, Francesco Quaglio, Lorenzo Tarocchi, Enea Tentoni, Andrea Gustinelli

{"title":"First Report of Philometroides sanguineus (Nematoda: Philometridae) in Farmed Goldfish (Carassius auratus) in Italy.","authors":"Teresa Pirollo, Perla Tedesco, Marialetizia Fioravanti, Francesco Quaglio, Lorenzo Tarocchi, Enea Tentoni, Andrea Gustinelli","doi":"10.1111/jfd.14138","DOIUrl":"https://doi.org/10.1111/jfd.14138","url":null,"abstract":"The ornamental fish trade has facilitated the global dissemination of parasites, posing significant biosecurity risks. This study documents the first detection of the nematode Philometroides sanguineus in farmed goldfish (Carassius auratus) in Emilia-Romagna, Italy. Specimens of gravid females, found in the caudal fins, and males, collected from the swim bladder, were identified through morphological, molecular and scanning electron microscope (SEM) analyses. This report underscores the risk of nonnative pathogen introduction via ornamental fish translocation, and the possibility of their spreading through the aquatic environment. In fact, the role of intermediate hosts, such as copepods, in parasite dissemination emphasises the need for stricter controls in systems reliant on surface water. Although P. sanguineus infection did not cause significant mortality in adult fish, its presence diminishes the ornamental value of infected specimens and raises concerns about the parasite's spread to native ecosystems. This case highlights the need to improve the biosecurity measures and comprehensive risk assessments to mitigate the introduction and propagation of alien parasites in aquaculture systems. Further research is necessary to evaluate the parasite's epidemiology, distribution and potential pathogenicity under different environmental and farming conditions.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14138"},"PeriodicalIF":2.2,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulation of Sodium and Ammonia Transporters in the Context of Viral Gill Diseases in Common Carp (Cyprinus carpio). 鲤病毒性鳃病中钠和氨转运体的调节。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-04-23 DOI: 10.1111/jfd.14133

Maria Zawisza, Muhammad Abdullah, Magdalena Marcinkowska, Marek Matras, Krzysztof Rakus, Mikolaj Adamek

{"title":"Modulation of Sodium and Ammonia Transporters in the Context of Viral Gill Diseases in Common Carp (Cyprinus carpio).","authors":"Maria Zawisza, Muhammad Abdullah, Magdalena Marcinkowska, Marek Matras, Krzysztof Rakus, Mikolaj Adamek","doi":"10.1111/jfd.14133","DOIUrl":"https://doi.org/10.1111/jfd.14133","url":null,"abstract":"Osmoregulation and ammonia removal are among key physiological processes that take place in gills and affect fish homeostasis and well-being. These processes can be disrupted by numerous environmental factors, but also by viral infections, especially those leading to severe gill disorders. The mechanisms of how viruses disrupt osmoregulation and ammonia removal in fish have not been extensively studied. We propose further exploration of the molecular and functional basis of viral induced gill disorders by studying the gene expression and enzyme activity of Na+/K+-ATPases (NKA) and ammonia transporters in the gills and kidney of common carp during infection with two viruses: carp edema virus (CEV) and cyprinid herpesvirus 3 (CyHV-3). In the case of NKA, the expression of subunit α of selected NKA was affected by both viruses; however, no discernible trends were observed in the gills and kidneys. The enzyme activity of NKA was significantly reduced in the gills during infection with both CEV and CyHV-3. Moreover, our immunohistochemical studies showed that during infection with CEV and CyHV-3, NKA-rich cells are transferred from the primary lamellae to the more superficial space and to the secondary lamellae of the gills. In the case of ammonia transporters, both CEV and CyHV-3 infection resulted in downregulation of the expression of major transporters gdh1, Rhag, and Rhbg, allowing us to partly explain the ammonia accumulation in blood observed during infection with these viruses. This study highlighted that virus induced gill disorders may lead to disruption of osmoregulation and ammonia removal by dysregulating the expression and activity of NKA and ammonia transporters.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14133"},"PeriodicalIF":2.2,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a Real-Time PCR Assay for Noninvasive Detection of Miamiensis avidus in Olive Flounder Aquaculture. 橄榄比目鱼养殖中巨斑迈阿密虫实时荧光定量PCR无创检测方法的建立。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-04-23 DOI: 10.1111/jfd.14134

Jin-Young Kim, Su-Mi Shin, Se Ryun Kwon, Sung-Ju Jung

{"title":"Development of a Real-Time PCR Assay for Noninvasive Detection of Miamiensis avidus in Olive Flounder Aquaculture.","authors":"Jin-Young Kim, Su-Mi Shin, Se Ryun Kwon, Sung-Ju Jung","doi":"10.1111/jfd.14134","DOIUrl":"https://doi.org/10.1111/jfd.14134","url":null,"abstract":"Miamiensis avidus (syn. Philasterides dicentrarchi) leads to high mortality and economic losses in olive flounder (Paralichthys olivaceus) aquaculture. In this study, we developed a real-time PCR assay targeting the cytochrome c oxidase subunit I (cox1) gene of M. avidus to sensitively detect and quantify the parasite in seawater. The assay showed a strong linear correlation between the log copy number of the standard plasmid and Ct values (R2 = 0.9985), achieving an amplification efficiency of 96.26%. The cox1 gene copy number was estimated at 6017 ± 2794 copies per cell. The assay specifically detected M. avidus (cox1 genotypes 1-4) without cross-reacting with Cryptocaryon irritans or Uronema elegans, identifying as few as two M. avidus cells in 1 L of seawater with 100% accuracy (Ct value: 31.95 ± 0.78). The noninvasive seawater sampling demonstrates higher sensitivity than invasive tissue sampling. A high density of M. avidus (57.81 cells/10 L) was detected in tanks housing olive flounder with clinical signs of infection, and sporadic detection occurred in both inflow and effluent seawater. The developed real-time PCR assay provides a sensitive, specific and noninvasive method for detecting and quantifying M. avidus, enabling improved disease prevention and management in fish farms.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14134"},"PeriodicalIF":2.2,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143986077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0