Journal of fish diseases最新文献_第2页

Histopathology in Aquatic Animal Health Sciences: Progress in Comparative Pathobiology.

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-01-25 DOI: 10.1111/jfd.14083

David J Speare, Barbara F Nowak, Heike Schmidt-Posthaus, Saengchan Senapin

引用次数: 0

A TaqMan-MGB Probe Quantitative PCR Assay Detecting Hematodinium perezi. TaqMan-MGB探针定量PCR检测perezi血液病。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-01-20 DOI: 10.1111/jfd.14082

Guosi Xie, Hailiang Wang, Mengting Zhu, Jingnan Bi, Liuxin Yang, Xiaoyuan Wan, Meng Li, Enpei Xie, Chengyin Shi, Bing Yang, Qingli Zhang, Caiwen Li, Jie Huang

{"title":"A TaqMan-MGB Probe Quantitative PCR Assay Detecting Hematodinium perezi.","authors":"Guosi Xie, Hailiang Wang, Mengting Zhu, Jingnan Bi, Liuxin Yang, Xiaoyuan Wan, Meng Li, Enpei Xie, Chengyin Shi, Bing Yang, Qingli Zhang, Caiwen Li, Jie Huang","doi":"10.1111/jfd.14082","DOIUrl":"https://doi.org/10.1111/jfd.14082","url":null,"abstract":"Hematodinium perezi, a pathogenic dinoflagellate, is one of major epidemiological agents that lead to severe losses of cultured marine crustaceans in China. This study aimed to develop a novel, sensitive and specific detection method qualified for early surveillance and control of the disease caused by H. perezi. The present study established a TaqMan-MGB probe quantitative PCR (qPCR) method, targeting the first internal transcribed spacer 1 (ITS 1) region of H. perezi by optimising reaction components. A high correlation coefficient (R2 = 0.9996) was obtained in a standard curve with a 103.4% efficiency. No amplification was observed for the host's genome and pathogens other than H. perezi in the TaqMan-MGB probe qPCR assays, showing high specificity to H. perezi. When using the plasmid standard DNA as templates, the detection limit of the qPCR method was determined to be 5.66 copies/reaction and 10 times more sensitive than the conventional PCR. The TaqMan-MGB probe qPCR assays exhibited high repeatability, and the intra- and inter-assay coefficients of variation (CV) ranged from 0.11% to 2.25% over a wide dynamic range of detection from 5.66 × 100 to 5.66 × 109 copies of targeting gene. The application was also validated on clinical samples, including those with low infection with H. perezi. This novel one-step TaqMan-MGB probe qPCR provides an option for surveillance and epidemiological investigations of H. perezi infection, with an advantage at the early infection stage.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14082"},"PeriodicalIF":2.2,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beating Cardiac Cell Cultures From Different Developmental Stages of Rainbow Trout as a Novel Approach for Replication of Cardiac Fish Viruses. 虹鳟鱼不同发育阶段跳动心脏细胞培养作为心脏鱼病毒复制的新途径。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-01-17 DOI: 10.1111/jfd.14080

Torben Krebs, Julia Bauer, Sarah Graff, Lukas Teich, Markus Sterneberg, Marina Gebert, Henrike Seibel, Bettina Seeger, John Hellmann, Øystein Wessel, Espen Rimstad, Win Surachetpong, Dieter Steinhagen, Verena Jung-Schroers, Mikolaj Adamek

{"title":"Beating Cardiac Cell Cultures From Different Developmental Stages of Rainbow Trout as a Novel Approach for Replication of Cardiac Fish Viruses.","authors":"Torben Krebs, Julia Bauer, Sarah Graff, Lukas Teich, Markus Sterneberg, Marina Gebert, Henrike Seibel, Bettina Seeger, John Hellmann, Øystein Wessel, Espen Rimstad, Win Surachetpong, Dieter Steinhagen, Verena Jung-Schroers, Mikolaj Adamek","doi":"10.1111/jfd.14080","DOIUrl":"https://doi.org/10.1111/jfd.14080","url":null,"abstract":"Piscine orthoreovirus-1 and 3 (PRV-1, PRV-3) cause highly prevalent infection in cultured salmonids and can induce heart and skeletal muscle inflammation (HSMI) resulting in economic losses in aquaculture. However, to date, PRV-1 and PRV-3 have withstood replication in continuous cell lines. In this study, we used beating heart cell cultures obtained from different developmental stages of rainbow trout (Oncorhynchus mykiss) (RTC-L and RTC-A) and tested their ability to sustain replication of PRV-1 and PRV-3. Furthermore, we compared the replication pattern of the different viruses with those in the newly developed heart fibroblast cell line (RTH-F) and the traditional established rainbow trout gonad cell line (RTG-2). Neither RTCs nor RTH-F cell lines supported replication of PRV-1 and PRV-3. Comparative experiments showed varying susceptibility of the novel cultures to viral haemorrhagic septicaemia virus (VHSV), chum salmon reovirus (CSV), infectious pancreatic necrosis virus (IPNV), piscine myocarditis virus (PMCV), salmonid alphavirus 3 (SAV-3) and tilapia lake virus (TiLV), indicating their usability for work with multiple fish viruses. While confirming the difficulty of replicating PRV-1 and PRV-3, the results demonstrate the potential of novel heart-derived cell cultures as in vitro tools for studying fish viruses.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14080"},"PeriodicalIF":2.2,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Conservation Genomics to Investigate the Role of Pathogens on the Migration of Sea-Run Brown Trout (Salmo trutta). 应用保护基因组学研究海褐鳟（Salmo trutta）迁移过程中病原体的作用。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-01-08 DOI: 10.1111/jfd.14045

Robert J Lennox, Sindre H Eldøy, Angela D Schulze, Kristina M Miller, Trond Einar Isaksen, Jan G Davidsen, Cecilie I Nilsen, Lotte S Dahlmo, Knut Wiik Vollset

{"title":"Application of Conservation Genomics to Investigate the Role of Pathogens on the Migration of Sea-Run Brown Trout (Salmo trutta).","authors":"Robert J Lennox, Sindre H Eldøy, Angela D Schulze, Kristina M Miller, Trond Einar Isaksen, Jan G Davidsen, Cecilie I Nilsen, Lotte S Dahlmo, Knut Wiik Vollset","doi":"10.1111/jfd.14045","DOIUrl":"https://doi.org/10.1111/jfd.14045","url":null,"abstract":"Pathogens play a key role in individual function and the dynamics of wild populations, but the link between pathogens and individual performance has rarely been investigated in the wild. Migrating salmonids offer an ideal study system to investigate how infection with pathogens affects performance given that climate change and fish farming portend increasing prevalence of pathogens in wild populations. To test for effects of pathogen burden on the performance of a migrating salmonid, we paired data from individual brown trout tagged with acoustic accelerometer transmitters and gill biopsies to investigate how pathogen infection affected whole animal activity during the spawning migration. Generalised additive models fitted to the acceleration data revealed individual and temporal variation in acceleration as expected, but also provided a significant effect of relative infection burden on acceleration. However, when linking this pathogen-specific effect to a relevant bioenergetic change, it was evident that the effect had little impact on the exercise-related oxygen consumption at the individual level, especially in cases where fish were not exerting high exercise activity. The results are a powerful example of how pairing non-lethal biopsies with individual tracking technologies can be used to assess how pathogens impact fish in situ.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14045"},"PeriodicalIF":2.2,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Potentially Novel Mycobacterium Spp. In Freshwater Ornamental Fish in Trinidad and Tobago. 特立尼达和多巴哥淡水观赏鱼中潜在新型分枝杆菌的鉴定。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-01-07 DOI: 10.1111/jfd.14079

Lemar Blake, Arianne Brown Jordan, Soren Nicholls, Esteban Soto, Luke Iwanowicz, Rod Suepaul, Christopher Oura, Ayanna Carla N Phillips Savage

{"title":"Identification of Potentially Novel Mycobacterium Spp. In Freshwater Ornamental Fish in Trinidad and Tobago.","authors":"Lemar Blake, Arianne Brown Jordan, Soren Nicholls, Esteban Soto, Luke Iwanowicz, Rod Suepaul, Christopher Oura, Ayanna Carla N Phillips Savage","doi":"10.1111/jfd.14079","DOIUrl":"https://doi.org/10.1111/jfd.14079","url":null,"abstract":"Potentially zoonotic Mycobacterium spp. are impacting freshwater ornamental fish in Trinidad and Tobago. Clinical cases presented at the Aquatic Animal Health Unit of The University of the West Indies, School of Veterinary Medicine, from September 2011 to September 2018 indicated the presence of piscine mycobacteriosis in freshwater ornamental fish from locations throughout Trinidad and Tobago. Subsequently, an investigation was conducted from June 2018 to December 2021 to identify the specific Mycobacterium spp. involved. Nested polymerase chain reaction (PCR) was used to amplify and later sequence the 723 bp of the rpoB. Analysis of region V of the rpoB offers similar discriminatory power as concatenation or whole genome analysis of Mycobacterium spp., and serves as an acceptable method for differentiating and discriminating between species in this genus. With respect to tank-reared freshwater ornamental fish, this study identified M. fortuitum (98.8% identity), M. liflandii (100% identity), M. stomatepiae (97.2% identity), M. pseudoshottsii related (96.9% identity), two M. stomatepiae related (95.4% and 96.7% identity), M. immunogenum related (93.4% identity), and 17 M. insubricum related (91.4%-95.2% identity). Additionally, seven mycobacteria related to M. insubricum were identified in wild-caught guppies. These findings suggest that both known, and potentially novel Mycobacterium spp. are circulating, and adversely impacting the local populations of freshwater ornamental fish in Trinidad and Tobago.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14079"},"PeriodicalIF":2.2,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

String Jellyfish Apolemia spp. (Most Likely A. uvaria) Causes Serious Tissue Damage in Farmed Fish. 绳水母Apolemia spp（很可能是A. uvaria）会对养殖鱼类造成严重的组织损伤。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2024-12-30 DOI: 10.1111/jfd.14077

Mette Hofossæter, Marta Alarcón, Helene Wisløff

引用次数: 0

The First Report of Cod Gill Poxvirus in Gills of Atlantic Cod (Gadus morhua L.) Suffering From Cardiorespiratory Disease. 大西洋鳕鱼（Gadus morhua L.）鳃中存在鳕鱼鳃痘病毒的首次报道患有心肺疾病。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2024-12-27 DOI: 10.1111/jfd.14078

Mona C Gjessing, Torstein Tengs, Hanne Nilsen, Saima Mohammad, Simon C Weli

{"title":"The First Report of Cod Gill Poxvirus in Gills of Atlantic Cod (Gadus morhua L.) Suffering From Cardiorespiratory Disease.","authors":"Mona C Gjessing, Torstein Tengs, Hanne Nilsen, Saima Mohammad, Simon C Weli","doi":"10.1111/jfd.14078","DOIUrl":"https://doi.org/10.1111/jfd.14078","url":null,"abstract":"Atlantic cod farming experiences renewed growth in Norway, and increased awareness is essential to address emerging diseases in this species. There are few reports on gill diseases in cod, and to date, no viral gill infections of cod have been documented. In this study, we collected samples from three sequential time points in summer 2023 from farmed cod suffering from cardiorespiratory disease. We document severe heart and gill pathology, and a novel double-strand DNA virus was discovered in the gills. Through comprehensive genetic characterisation and comparative sequence analysis, this virus was classified as a new species in the genus Poxvirus, designated cod gill poxvirus (CGPV). We demonstrate disease causality with severe gill lesions as shown by histopathology and RNA scope in situ hybridisation, and poxvirus particles were identified in gill epithelial cells by transmission electron microscopy. Further, another gill pathogen not previously described in cod, Candidatus. Branchiomonas cysticola, was identified by pcr and in situ hybridisation. Our findings provide strong evidence for poxvirus in Atlantic cod and underscore the imminent threat posed to Atlantic cod farm industry.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14078"},"PeriodicalIF":2.2,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and Characterisation of an Immortal Cell Line From Largemouth Bass (Micropterus salmoides) for Viral Studies. 用于病毒研究的大口黑鲈（Micropterus salmoides）不朽细胞系的发育和特性。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2024-12-24 DOI: 10.1111/jfd.14071

Qianyi Mai, Qing Wang, Yingying Li, Jiyuan Yin, Xubing Mo, Cunbin Shi, Yan Ren, Donghai Liu, Xuanming Liu, Dongli Sun, Weiqiang Liu, Yuqi Jin, Weiwei Zeng, Yingying Wang

{"title":"Development and Characterisation of an Immortal Cell Line From Largemouth Bass (Micropterus salmoides) for Viral Studies.","authors":"Qianyi Mai, Qing Wang, Yingying Li, Jiyuan Yin, Xubing Mo, Cunbin Shi, Yan Ren, Donghai Liu, Xuanming Liu, Dongli Sun, Weiqiang Liu, Yuqi Jin, Weiwei Zeng, Yingying Wang","doi":"10.1111/jfd.14071","DOIUrl":"https://doi.org/10.1111/jfd.14071","url":null,"abstract":"Largemouth bass (Micropterus salmoides), a freshwater fish species, is cultivated widely across China. The industry has been greatly affected by various viral diseases. We generated a new immortal cell line from the fin of M. salmoides (MSF). It has been successfully maintained in continuous culture for over 80 passages. Cells multiply well at 28°C using Medium 199 or Leibovitz's L-15 supplemented with 10%-20% fetal bovine serum. MSF cells comprise a mass of epithelialoid cells. Chromosome analysis showed that ploidy at passage 55 was 2n = 58. Amplification and analysis of the sequence of cytochrome oxidase I gene ascertained the origin of MSF cells from M. salmoides. The cell line is devoid of contamination with mycoplasma. Successful transfection with a GFP reporter gene indicated that the MSF cell line can be a useful tool for further gene expression studies. MSF cells exhibited susceptibility to Largemouth bass ranavirus (LMBV), Infectious spleen and kidney necrosis virus (ISKNV), and Siniperca chuatsi rhabdovirus (SCRV). Transmission electron microscopy (TEM), and immunofluorescence confirmed that the MSF cell line showed high susceptibility to all the tested viruses. The titre values of MSF after infection with LMBV, ISKNV, and SCRV reached 107.4 TCID50/mL, 106.15 TCID50/mL, and 106.35 TCID50/mL at 7dpi, respectively. In conclusion, an immortal cell line derived from the fin tissue of M. salmoides was successfully developed and certain fish viruses can effectively propagated in the newly established MSF cell line that can be helpful for future gene manipulation and infectious viral mechanistic studies in the future.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14071"},"PeriodicalIF":2.2,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid Visual Detection of Red Sea Bream Iridovirus Using a Novel Cross-Priming Amplification-Based Lateral Flow Assay. 基于交叉引物扩增横向流动试验的红鲷虹膜病毒快速视觉检测

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2024-12-21 DOI: 10.1111/jfd.14073

Guk Hyun Kim, Dong Jun Shin, Ji Yeong Choi, Hyun Deok Choi, Joon Gyu Min, Kwang Il Kim

{"title":"Rapid Visual Detection of Red Sea Bream Iridovirus Using a Novel Cross-Priming Amplification-Based Lateral Flow Assay.","authors":"Guk Hyun Kim, Dong Jun Shin, Ji Yeong Choi, Hyun Deok Choi, Joon Gyu Min, Kwang Il Kim","doi":"10.1111/jfd.14073","DOIUrl":"https://doi.org/10.1111/jfd.14073","url":null,"abstract":"Red sea bream iridovirus (RSIV) occurs mainly at high water temperatures and infects more than 30 different species of fish. In Asia, infected fish cause mass mortality every year. Molecular diagnostics is a technology that efficiently detects and identifies a wide range of fish pathogens through rapid and sensitive analysis of their genetic material. Rapid viral detection is essential for effective disease control. In this study, we developed and validated a cross-priming amplification-based lateral flow assay (CPA-LFA) suitable for field diagnosis owing to its short diagnostic time and simple diagnostic process. The CPA-LFA achieved optimal performance with concentrations of 4 mM MgSO4, 1.2 mM dNTPs and 0.7 M betaine, with the reaction conducted at 60°C for 60 min. The developed CPA-LFA could specifically identify RSIV without cross-reactivity with several pathogens commonly reported in various fish cell lines and fish. The 95% limit of detection (LOD95%) of CPA-LFA was 385.76 copies/μL, which was comparable to that of conventional polymerase chain reaction (PCR). Quantitative PCR (qPCR) was used to identify true-positive and true-negative samples from 210 fish samples (160 from cultured fish and 50 from artificially infected fish). Compared with qPCR, CPA-LFA classified six positive samples as false positives. The viral load of these samples was determined to be less than 195.1 copies/μL. The diagnostic sensitivity and specificity of CPA-LFA were 94.34% and 100%, respectively. Furthermore, inter-operator reproducibility testing yielded a kappa value of 1.0, indicating perfect agreement. Therefore, the novel CPA-LFA is especially well-suited for field diagnostics owing to its straightforward diagnostic procedure and capability to quickly and accurately detect RSIV.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14073"},"PeriodicalIF":2.2,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loop-Mediated Isothermal Amplification Combined With Lateral-Flow Dipstick for Detection of Centrocestus formosanus in Ornamental Fish. 环介导等温扩增结合侧流试纸法检测观赏鱼中台山心螺。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2024-12-17 DOI: 10.1111/jfd.14074

Metawee Sabaijai, Thanawan Tejangkura, Thapana Chontananarth

{"title":"Loop-Mediated Isothermal Amplification Combined With Lateral-Flow Dipstick for Detection of Centrocestus formosanus in Ornamental Fish.","authors":"Metawee Sabaijai, Thanawan Tejangkura, Thapana Chontananarth","doi":"10.1111/jfd.14074","DOIUrl":"https://doi.org/10.1111/jfd.14074","url":null,"abstract":"The minute intestinal trematode Centrocestus formosanus is a major problem that can be found in the gills of various fish species. This parasite can destroy the gill structure of fish, leading to increased fish morbidity and mortality rates. In this study, loop-mediated isothermal amplification (LAMP) based on the internal transcript spacer 1 region combined with a lateral-flow dipstick (LAMP-LFD) assay was used to detect C. formosanus in goldfish, Carassius auratus. The results showed that LAMP-LFD was specific to C. formosanus and had no cross-amplification with other co-infecting parasite species, close related parasite species or their hosts (both intermediate host and definitive host). The limit of detection is as low as one metacercaria per gill. In testing 40 goldfish gill samples, the LAMP-LFD method showed 100% accuracy when compared to traditional morphological identification. This method can be used as a rapid diagnostic tool for C. formosanus detection to obtain epidemiological information for monitoring, controlling, and preventing outbreaks of this parasite.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14074"},"PeriodicalIF":2.2,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142837089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0