Journal of fish diseases最新文献_第5页

Divergent Healing Trajectories Among Common Mechanical Skin Wound Types in Post-Smolt Atlantic Salmon (Salmo salar Linnaeus, 1758). 大西洋鲑鱼产后常见机械皮肤伤口类型的不同愈合轨迹（Salmo salar Linnaeus, 1758）。

IF 2.3 3区农林科学

Journal of fish diseases Pub Date : 2026-09-15 DOI: 10.1111/jfd.70290

Kasidis Chaiyasut, Rose Ruiz Daniels, Christian Karlsen, Barry Bradford, Gunhild Seljehaug Johansson, Miroslava Hansen, Marta Bou, Marianne Vaadal, Lene Rydal Sveen

{"title":"Divergent Healing Trajectories Among Common Mechanical Skin Wound Types in Post-Smolt Atlantic Salmon (Salmo salar Linnaeus, 1758).","authors":"Kasidis Chaiyasut, Rose Ruiz Daniels, Christian Karlsen, Barry Bradford, Gunhild Seljehaug Johansson, Miroslava Hansen, Marta Bou, Marianne Vaadal, Lene Rydal Sveen","doi":"10.1111/jfd.70290","DOIUrl":"https://doi.org/10.1111/jfd.70290","url":null,"abstract":"Mechanical wounds are a common form of skin damage in Atlantic salmon (Salmo salar Linnaeus, 1758) aquaculture and can predispose fish to secondary infections. Such wounds often arise from routine farming practices, including delousing operations. Although visually similar, different types of mechanical injury may trigger distinct healing processes, which remain poorly understood. To investigate this, post-smolt Atlantic salmon were subjected to three wound models: scale loss, superficial wounds involving the epidermis and dermis, and deep wounds extending through all skin layers. Responses were assessed at 2 days and 5 weeks post-wounding to capture different phases of skin wound healing. The injuries were characterised using serum parameters, stereomicroscopy, histology, immunohistochemistry for cell proliferation, quantitative polymerase chain reaction (qPCR), and RNAscope analysis of T cell markers. Despite their initially similar appearance, scale loss and superficial lesions followed markedly different healing trajectories. Fish with scale removal mostly recovered to the pre-damage state within 5 weeks, whereas superficial damage elicited a delayed healing response similar to that of deep wounds. Each wound type exhibited distinct epidermal and hypodermal alterations, highlighting differences among wound types that should be considered by fish health personnel and farmers.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70290"},"PeriodicalIF":2.3,"publicationDate":"2026-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148977138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First Report of Halomonhystera sp. cf. disjuncta Associated With Branchial and Cutaneous Lesions During a Mortality Event in Laboratory-Acclimated Olive Flounder (Paralichthys olivaceus). 在实验室驯化的橄榄比目鱼（橄榄比目鱼）死亡事件中，与鳃裂和皮肤病变相关的嗜盐性牙鲆的首次报道。

IF 2.3 3区农林科学

Journal of fish diseases Pub Date : 2026-09-13 DOI: 10.1111/jfd.70287

Hyo-Young Kang, Ju-Yeop Lee, Young-Ung Heo, Cho-Yoon Shin, Thanh Luan Nguyen, Do-Hyung Kim

{"title":"First Report of Halomonhystera sp. cf. disjuncta Associated With Branchial and Cutaneous Lesions During a Mortality Event in Laboratory-Acclimated Olive Flounder (Paralichthys olivaceus).","authors":"Hyo-Young Kang, Ju-Yeop Lee, Young-Ung Heo, Cho-Yoon Shin, Thanh Luan Nguyen, Do-Hyung Kim","doi":"10.1111/jfd.70287","DOIUrl":"https://doi.org/10.1111/jfd.70287","url":null,"abstract":"This clinical report describes a mortality event associated with a free-living marine nematode provisionally assigned to the Halomonhystera disjuncta species complex (Halomonhystera sp. cf. disjuncta) in laboratory-acclimated olive flounder (Paralichthys olivaceus). Fish were acclimated at 13°C in filtered seawater with 50% daily water exchange. Affected fish developed caudal fin erosion, gill pallor, excessive branchial mucus, abnormal posture and, in larger fish, cutaneous ulcers. Cumulative losses within 1 month reached approximately 50% in the 10 g groups and 30% in the 100 g group. Microscopy revealed numerous nematodes in branchial mucus, adjacent to gill lamellae and in fin erosions and skin ulcers. Morphologically similar nematodes were recovered from the seawater filtration line but were not molecularly confirmed. VHSV was not detected by RT-qPCR at stocking or during the mortality event, and outbreak-associated PCR assays yielded no target-specific amplification for Tenacibaculum maritimum, Cryptocaryon irritans, or Ichthyobodo spp. Partial 18S rRNA and ITS sequences showed 100% identity to GenBank sequences assigned to H. disjuncta. Because this complex contains cryptic lineages, the nematode was conservatively designated Halomonhystera sp. cf. disjuncta. Although causality was not established, the findings support a clinicopathological association with branchial and cutaneous lesions.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70287"},"PeriodicalIF":2.3,"publicationDate":"2026-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148963107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and Characterisation of Aeromonas hydrophila, Aeromonas veronii and Vibrio cholerae O139 From Diseased Bester Sturgeon (Huso huso × Acipenser ruthenus): A Case Report. 病鲟嗜水气单胞菌、维氏气单胞菌和霍乱弧菌O139的分离与鉴定1例

IF 2.3 3区农林科学

Journal of fish diseases Pub Date : 2026-09-11 DOI: 10.1111/jfd.70280

Petya Stefanova Orozova, Dimitar Petrov Ivanov, Deyan Stratev Stratev, Radostin Stefanov Simeonov, Ivelina Hubenova Grozeva, Pavel Istiliyanov Rashev

{"title":"Isolation and Characterisation of Aeromonas hydrophila, Aeromonas veronii and Vibrio cholerae O139 From Diseased Bester Sturgeon (Huso huso × Acipenser ruthenus): A Case Report.","authors":"Petya Stefanova Orozova, Dimitar Petrov Ivanov, Deyan Stratev Stratev, Radostin Stefanov Simeonov, Ivelina Hubenova Grozeva, Pavel Istiliyanov Rashev","doi":"10.1111/jfd.70280","DOIUrl":"https://doi.org/10.1111/jfd.70280","url":null,"abstract":"This study investigated bacterial pathogens associated with disease and mortality in farmed bester sturgeon (Huso huso × Acipenser ruthenus) reared in freshwater cages in the Vacha Reservoir, Bulgaria. Affected fish exhibited lethargy, anorexia, haemorrhagic skin lesions and multifocal internal pathology. Histological examination revealed hepatic degeneration and vacuolar change, consistent with systemic bacterial infection. Bacterial isolates recovered from blood and internal organs were identified as Aeromonas hydrophila, A. veronii and Vibrio cholerae O139 using culture-based methods, MALDI-TOF MS, species-specific PCR and 16S rRNA gene sequencing. Virulence profiling showed that A. hydrophila carried multiple virulence-associated and enterotoxin genes, whereas A. veronii possessed only aerA. The V. cholerae O139 isolate lacked ctxA and rfbO1, confirming a non-toxigenic profile. In vivo infection trials with clinical sturgeon isolates A. hydrophila HGM24 and V. cholerae O139 GM24 produced dose-dependent mortality, demonstrating the pathogenic potential of both isolates. This case represents the first concurrent detection and characterisation of A. hydrophila, A. veronii and V. cholerae O139 in bester sturgeon in Bulgaria. It underscores the importance of integrated diagnostic approaches combining pathogen identification, virulence assessment, histopathology and experimental infection in freshwater aquaculture disease investigations.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70280"},"PeriodicalIF":2.3,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148956021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a Multiplex Polymerase Chain Reaction Assay for Differentiating Three Lactococcus Species Associated With Piscine Lactococcosis. 建立多重聚合酶链式反应法鉴别与鱼乳球菌病相关的三种乳球菌。

IF 2.3 3区农林科学

Journal of fish diseases Pub Date : 2026-09-09 DOI: 10.1111/jfd.70288

Tomoaki Yoshino, Tomokazu Takano, Hiroki Tanno, Yukihiro Nakagawa, Yuta Matsuura, Shogo Harakawa, Hidehiro Kondo, Ikuo Hirono, Keiichiro Koiwai, Tomomasa Matsuyama

{"title":"Development of a Multiplex Polymerase Chain Reaction Assay for Differentiating Three Lactococcus Species Associated With Piscine Lactococcosis.","authors":"Tomoaki Yoshino, Tomokazu Takano, Hiroki Tanno, Yukihiro Nakagawa, Yuta Matsuura, Shogo Harakawa, Hidehiro Kondo, Ikuo Hirono, Keiichiro Koiwai, Tomomasa Matsuyama","doi":"10.1111/jfd.70288","DOIUrl":"https://doi.org/10.1111/jfd.70288","url":null,"abstract":"Piscine lactococcosis is an important bacterial disease of farmed fish. The causative agents, Lactococcus garvieae, Lactococcus petauri and Lactococcus formosensis, are closely related, which complicates species-level identification. We developed a conventional multiplex PCR assay targeting species-specific genes identified by comparative genomic analysis. Average nucleotide identity reassignment of 441 publicly available genome assemblies identified 111 L. garvieae, 255 L. petauri and 75 L. formosensis genomes. Species-specific primers and a tuf-based Lactococcus common control were evaluated using in silico polymerase chain reaction (PCR) against target genomes and 10,460 off-target assemblies representing 474 taxa in 12 genera. Experimental specificity was assessed using six target strains and 19 non-target fish pathogens. Distinct amplicons of 195, 333 and 500 bp were produced for L. garvieae, L. petauri and L. formosensis, respectively, together with a 132-bp control amplicon. No cross-amplification was observed. All target species were detected in mixed-DNA samples and spiked kidney and spleen homogenates from two fish species. Analytical detection limits were estimated based on microscopic cell counts of bacterial suspensions before DNA extraction and ranged from 0.956 to 8.55 cell equivalents per reaction. This assay represents a rapid, low-cost method for differentiating lactococcosis-causing Lactococcus species using standard PCR and agarose gel electrophoresis.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70288"},"PeriodicalIF":2.3,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148912386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Rapid RPA-CRISPR/Cas12a Approach for Detecting Mycobacterium marinum. 一种快速检测海洋分枝杆菌的RPA-CRISPR/Cas12a方法

IF 2.3 3区农林科学

Journal of fish diseases Pub Date : 2026-09-07 DOI: 10.1111/jfd.70286

Guangda Yan, Hongxia Li, Liwen Xu, Huajian Lin, Yiqin Deng, Zhixun Guo, Hongling Ma, Juan Feng

{"title":"A Rapid RPA-CRISPR/Cas12a Approach for Detecting Mycobacterium marinum.","authors":"Guangda Yan, Hongxia Li, Liwen Xu, Huajian Lin, Yiqin Deng, Zhixun Guo, Hongling Ma, Juan Feng","doi":"10.1111/jfd.70286","DOIUrl":"https://doi.org/10.1111/jfd.70286","url":null,"abstract":"Mycobacterium marinum is one of the most common atypical mycobacteria causing opportunistic infections in humans. It is considered to be one of the most important fish pathogens and is associated with a variety of diseases in freshwater and marine fish. In this study, a rapid detection method for M. marinum was established based on the Recombinase Polymerase Amplification (RPA) technology and the CRISPR/Cas12a trans-cut principle. By designing and screening RPA primers and crRNA, the RPA amplification system and CRISPR/Cas12a detection system were optimized, and an RPA-CRISPR/Cas12a detection system with high sensitivity and specificity was successfully constructed. The LOD95 of this detection method was estimated to be 5.7 copies/μL (95% CI: 2.4-15.5 copies/μL). The RPA-CRISPR assay showed perfect agreement with qPCR, with 100% sensitivity (95% CI: 76.8%-100%) and 100% specificity (95% CI: 73.5%-100%). The RPA-CRISPR/Cas12a-M. marinum detection method established in this study has the advantages of simple operation and visualization of results. It provides an effective tool for rapid on-site detection of M. marinum and is expected to play an essential role in the monitoring and prevention of aquaculture diseases.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70286"},"PeriodicalIF":2.3,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148905717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A CRISPR-Based Rapid Detection Assay for Crayfish Plague (Aphanomyces astaci) From Environmental Samples. 基于crispr的环境样品小龙虾鼠疫（Aphanomyces astaci）快速检测方法

IF 2.3 3区农林科学

Journal of fish diseases Pub Date : 2026-09-07 DOI: 10.1111/jfd.70278

Christopher Heine, Amadeus Plewnia, David Strand, Simran Gupta, Henrik Krehenwinkel, Gus R McFarlane

{"title":"A CRISPR-Based Rapid Detection Assay for Crayfish Plague (Aphanomyces astaci) From Environmental Samples.","authors":"Christopher Heine, Amadeus Plewnia, David Strand, Simran Gupta, Henrik Krehenwinkel, Gus R McFarlane","doi":"10.1111/jfd.70278","DOIUrl":"https://doi.org/10.1111/jfd.70278","url":null,"abstract":"Crayfish plague, caused by Aphanomyces astaci (Aa), is an infectious disease invasive in Europe, where its rapid spread has resulted in sharp declines of native crayfish species. Monitoring currently relies on a highly sensitive, but costly and time-consuming qPCR approach. Here, we designed a simplified, rapid and cost-efficient molecular assay for on-site detection of Aa. The novel rapid assay employs a combination of isothermal recombinase polymerase amplification and CRISPR-Cas12a-based detection that can be coupled with fluorescence or lateral flow visualisation. We demonstrate that the novel assay can detect A. astaci from tissue and environmental DNA with higher sensitivity than the available qPCR assay and readily distinguishes Aa from its non-pathogenic sister taxon A. fennicus. We tested two genomic marker sites for Aa that discriminate closely related oomycetes and incorporate field-deployable lateral flow and fluorescence readouts. Our work will make crayfish plague monitoring broadly accessible to practitioners and non-academic stakeholders as a tool to curb further Aa-driven loss of Europe's imperilled freshwater crustaceans and strengthen preparedness against future incursions of the pathogen in other regions.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70278"},"PeriodicalIF":2.3,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148900650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neoplastic Bone Lesions in Indian Oil Sardine (Sardinella longiceps): Apparent Prevalence, Morphological Diversity and Histopathological Insights. 印度油沙丁鱼（长尾沙丁鱼）的肿瘤性骨病变：明显的患病率，形态多样性和组织病理学见解。

IF 2.3 3区农林科学

Journal of fish diseases Pub Date : 2026-09-04 DOI: 10.1111/jfd.70285

Singaravel Vijayapoopathi, Sakthivel Muthu, Gopalakrishnan Ayyaru

{"title":"Neoplastic Bone Lesions in Indian Oil Sardine (Sardinella longiceps): Apparent Prevalence, Morphological Diversity and Histopathological Insights.","authors":"Singaravel Vijayapoopathi, Sakthivel Muthu, Gopalakrishnan Ayyaru","doi":"10.1111/jfd.70285","DOIUrl":"https://doi.org/10.1111/jfd.70285","url":null,"abstract":"This study provides a detailed pathological characterization of skeletal neoplasms in the Indian oil sardine (Sardinella longiceps) from the southeast coast of India. A total of 237,370 landed fish examined at Parangipettai landing center between 2022 and 2024 formed the surveillance population, among which 721 tumour cases were documented. The overall apparent prevalence of grossly detected tumour cases among landed fish examined was 0.30%, with seasonal values ranging from 0.02% during monsoon to 0.72% during summer. Among the tumour-bearing fish, 136 lesions showed skeletal involvement and constituted the focus of the present study. Grossly, these lesions appeared as firm, irregular bony swellings involving predominantly cranial and fin-associated skeletal regions, with lesion diameters ranging from 1.5 to 14.5 mm. Radiographic examination of skeletal cases demonstrated well-defined radiopaque lesions consistent with mineralized skeletal proliferation. Of the 136 skeletal lesions, 112 were suitable for histopathological examination. Histologically, 97 lesions (86.61%) were classified as compact (ivory) osteoma, 12 (10.71%) as cancellous osteoma, and 3 (2.68%) as chondrosarcoma. Osteomas were characterized by well-differentiated mature lamellar bone without cytological atypia or malignant features, whereas chondrosarcomas exhibited increased cellularity, nuclear atypia, a lobulated chondroid matrix, and focal infiltrative growth within the available sections. Because complete tumour margins and surrounding tissues were not available in all chondrosarcoma cases, the extent of invasion could not be comprehensively assessed. These findings provide baseline pathological evidence of skeletal neoplasia in S. longiceps and support continued pathological surveillance and further investigation into the biological and environmental factors potentially associated with tumour development in wild marine fish.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70285"},"PeriodicalIF":2.3,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148891653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid Detection of Spring Viremia of Carp Virus (SVCV) Using RAA-CRISPR/Cas12a. 利用RAA-CRISPR/Cas12a快速检测鲤鱼病毒春季病毒血症

IF 2.3 3区农林科学

Journal of fish diseases Pub Date : 2026-09-02 DOI: 10.1111/jfd.70284

Xiaoyang Li, Aoqing Zhang, Yingying Li, Yingying Wang, Hao Zheng, Defeng Zhang, Yan Ren, Xubing Mo, Yuting Deng, Junyu Luo, Meiling Yu, Bo Chen, Qing Wang, Jiyuan Yin

{"title":"Rapid Detection of Spring Viremia of Carp Virus (SVCV) Using RAA-CRISPR/Cas12a.","authors":"Xiaoyang Li, Aoqing Zhang, Yingying Li, Yingying Wang, Hao Zheng, Defeng Zhang, Yan Ren, Xubing Mo, Yuting Deng, Junyu Luo, Meiling Yu, Bo Chen, Qing Wang, Jiyuan Yin","doi":"10.1111/jfd.70284","DOIUrl":"https://doi.org/10.1111/jfd.70284","url":null,"abstract":"Spring viremia of carp virus (SVCV), that can infect cyprinoid and koi causing an acute hemorrhagic symptom, with a feature of widespread epidemic, which poses a severe threat to the global aquaculture industry. Given the absence of specific effective medicine or vaccines, the early, accurate diagnosis is critical in the disease prevention. Many SVCV detection methods have been established, but most require specialized equipment and skilled operators, which limits their field application. In this study, we established a recombinase-mediated isothermal amplification (RAA) in conjunction with the CRISPR/Cas12a system targeting the screened conserved region of the G gene in the genome to identify SVCV. The optimal combination of the primer pairs and crRNA was screened via the fluorescence intensity. In addition, the test exhibited high sensitivity and specificity, which could detect at the limit of 1.243 × 101 copies/μL and show no cross-reactivity with other common freshwater fish pathogens. Furthermore, the result could be visible by excitation of fluorophores by blue light at 39°C within 30 min. The integrated method combines the advantages of RAA and CRISPR-based signal transduction, offering a diagnosis project in resource-limited aquaculture environments and a field-applicable early warning of SVCV.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70284"},"PeriodicalIF":2.3,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148874373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-Specific Immune Response in Large Yellow Croaker (Larimichthys crocea) Experimentally Infected With Pseudomonas plecoglossicida by Intraperitoneal Injection or Immersion 大黄鱼腹腔注射或浸泡感染假单胞菌后的非特异性免疫反应。

IF 2.3 3区农林科学

Journal of fish diseases Pub Date : 2026-09-01 Epub Date: 2026-05-05 DOI: 10.1111/jfd.70201

Liangyin He, Rongguang Zhong, Doudou Wu, Junkun Wang, Xinling Xu, Weiqing Huang

{"title":"Non-Specific Immune Response in Large Yellow Croaker (Larimichthys crocea) Experimentally Infected With Pseudomonas plecoglossicida by Intraperitoneal Injection or Immersion","authors":"Liangyin He, Rongguang Zhong, Doudou Wu, Junkun Wang, Xinling Xu, Weiqing Huang","doi":"10.1111/jfd.70201","DOIUrl":"10.1111/jfd.70201","url":null,"abstract":"<div>\u0000 \u0000 The bacterial pathogen Pseudomonas plecoglossicida causes visceral white nodules disease (VWND) in the large yellow croaker (LYC; Larimichthys crocea), a commonly farmed species in China, resulting in substantial economic losses in the aquaculture industry. To better understand host innate immune mechanisms during P. plecoglossicida infection, LYCs were infected either by intraperitoneal (IP) injection or immersion, and their non-specific immune responses were compared. Fish were subjected to serial concentrations of the pathogen in both challenged groups, and the median lethal dose (LD50) was determined for subsequent infection trials. The results show that the respiratory burst activity, antioxidant activity (SOD, CAT and MDA), and non-specific immune responses (ACP, ALP and LZM) of LYCs were stimulated, and the expression levels of inflammatory cytokines (il-1β, tnfα1, il-8 and il-10) were considerably upregulated compared with mock-infected fish during bacterial infection periods via both challenge routes. Furthermore, the non-specific response in the immersion group started later and appeared to last longer, often exceeding the response in IP-injected fish. These results will contribute to a further understanding of the pathogenesis and host defence system in fishes following infection with P. plecoglossicida, and may inform the future development of preventive strategies for controlling VWND.\u0000 </div>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":"49 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First Report of Fibromyxoma in a Greater Amberjack (Seriola dumerili) From Aquarium of Genoa. 热那亚水族馆大琥珀鱼纤维黏液瘤首次报道。

IF 2.3 3区农林科学

Journal of fish diseases Pub Date : 2026-09-01 DOI: 10.1111/jfd.70277

Fabiano Capparucci, Maria Giovanna Piro, Andrea Martino, Gaia Pesce, Fabio Marino, Carmelo Iaria

{"title":"First Report of Fibromyxoma in a Greater Amberjack (Seriola dumerili) From Aquarium of Genoa.","authors":"Fabiano Capparucci, Maria Giovanna Piro, Andrea Martino, Gaia Pesce, Fabio Marino, Carmelo Iaria","doi":"10.1111/jfd.70277","DOIUrl":"https://doi.org/10.1111/jfd.70277","url":null,"abstract":"In teleosts, mesenchymal tumours like fibromas are frequently observed, whereas their malignant counterparts, fibrosarcomas, occur only sporadically. In our study, an adult female greater amberjack (Seriola dumerili) reared at the Aquarium of Genoa developed a slow-growing mass protruding from the ventral right side of the head. The tissue was firm and white greyish with localized cranial haemorrhaging on the external surface. Cut-section examination showed a homogeneous, dry, whitish and richly vascularized tissue layout. Tissue samples from the mass were stained with Masson's Trichrome, Alcian Blue-PAS (pH 2.5), and Toluidine blue for differential diagnosis. Microscopically, it was characterized by alternating collagenous spindle-cell septa and hypocellular metachromatic myxoid areas positive for acidic mucopolysaccharides and glycosaminoglycans. The absence of cellular atypia, mitotic activity and necrosis confirms the diagnosis of fibromyxoma. Documenting rare tumours in aquarium fish expands comparative pathology literature and highlights public aquaria as valuable research platforms for long-term health monitoring under controlled husbandry conditions.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70277"},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148864072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0