Journal of fish diseases最新文献

{"title":"Cyprinid Herpesvirus 2 Infection Activates IL-17C/NF-κB/IFNγ Antiviral Signalling Axis in Caudal Fin Cells of Carassius auratus Gibelio.","authors":"Yaqing Xie, Xiaomi Zhang, Feiran Li, Liqun Lu","doi":"10.1111/jfd.70017","DOIUrl":"https://doi.org/10.1111/jfd.70017","url":null,"abstract":"<p><p>Crucian carp (Carassius auratus gibelio) is sensitive to the infection of Cyprinid herpesvirus 2 (CyHV-2), displaying severe inflammatory reactions characterised by systemic congestion and gill bleeding. This study aimed to elucidate the mechanism by which CyHV-2 infection induces interferon gamma (IFNγ) expression in caudal fin cells (GiCF) of Carassius auratus gibelio, specifically investigating the role of the IL-17C/NF-κB signalling axis. In mammalian systems, virus-induced IL-17 has been demonstrated to activate NF-κB, a master regulator of inflammatory responses that controls multiple antiviral immune genes including interferons. Therefore, the induction mechanism of IFNγ gene expression following CyHV-2 infection was elucidated by revealing the interplay among IL-17C, NF-κB and IFNγ in this study. We demonstrated that CyHV-2 infection significantly upregulated the expression of IL-17C, NF-κB and IFNγ at both transcriptional and translational levels in GiCF. Furthermore, overexpression of IL-17C upregulated NF-κB and IFNγ expressions, while pharmacological inhibition of NF-κB (BAY 11-7082) suppressed this upregulation. This study provides the first evidence for the activation of IL-17C/NF-κB/IFNγ antiviral signalling axis in teleost fish in response to herpesvirus infection. Our findings highlight the dual role of virus-induced NF-κB activity, which not only orchestrates host defence (via IFNγ) but may also be exploited by the virus to potentially regulate its own gene transcription.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70017"},"PeriodicalIF":2.2,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144600642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insect Larvae Meal as a Complementary Functional Ingredient in High Soybean Meal-Based Diets Improve the Health of Rainbow Trout (Oncorhynchus mykiss).","authors":"Jie Ma, Krishna Pada Singha, Mosope F Abanikannda, Veronica Myrsell, Nicholas Romano, Elizabeth Koutsos, Daniel Adams, Kenneth D Cain, Vikas Kumar","doi":"10.1111/jfd.14153","DOIUrl":"https://doi.org/10.1111/jfd.14153","url":null,"abstract":"<p><p>This study explores the integration of black soldier fly larvae (BSFL) as a complementary functional ingredient to increase the soybean meal (SBM) inclusion in rainbow trout (Oncorhynchus mykiss) diets. Six experimental diets were formulated including a control diet as fishmeal (FM), an SBM-based diet, and 2.5% and 5% whole-body (WB) or defatted (DB) BSFL-supplemented SBM-based diets. Results revealed that BSFL inclusion positively influences gut health, immune response and survival rates following challenge with Flavobacterium psychrophilum. Dietary lauric acid content significantly impacted whole-body lauric acid levels in a dose-dependent manner, with BSFL diets showing higher levels than FM and SBM diets. Fish fed diets with BSFL exhibited enhanced survivability against F. psychrophilum infection compared to SBM-fed fish, with the highest survival rates observed in the WB5 (5% whole-body BSFL) group. Histological analysis demonstrated improved intestinal morphology in BSFL-fed fish, particularly evident in the absence of pathogenic enteritis. Gene expression analysis revealed upregulated proinflammatory markers (IL-8, TNF-α, C5) in BSFL-fed fish post-challenge, indicating an enhanced immune response. These findings indicate the potential of BSFL as a functional feed ingredient to complement high SBM inclusion in rainbow trout with improved health and performance. By strategically reducing inclusion levels and repositioning BSFL as a functional feed ingredient instead of fishmeal replacer in aquafeed formulations, one can mitigate economic concerns while maximising the benefits of this sustainable alternative.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14153"},"PeriodicalIF":2.2,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fantastic Flavos—An Introduction to the Special Issue, Flavobacteria: Current Status and Future Directions","authors":"Benjamin R. LaFrentz,&nbsp;Thomas P. Loch,&nbsp;Timothy J. Bruce","doi":"10.1111/jfd.70012","DOIUrl":"10.1111/jfd.70012","url":null,"abstract":"","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":"48 8","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jfd.70012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a Phage-Display Derived Peptide-Based Colloidal Gold Strip for Rapid Detection of Enterocytozoon hepatopenaei via Spore Wall Protein Interaction.","authors":"Jia-Rong Xie, Hao Li, Ben Chen, Yan-Chang Fan, Yu-Hao Gong, Liqun Lu","doi":"10.1111/jfd.14160","DOIUrl":"https://doi.org/10.1111/jfd.14160","url":null,"abstract":"<p><p>Infection by Enterocytozoon hepatopenaei (EHP) leads to stunted or arrested growth in shrimp, causing significant economic losses in shrimp aquaculture. In this study, the spore wall protein EhSW12 was expressed using a prokaryotic expression system. Subsequently, phage display technology was employed to screen for peptides that interact with EhSW12. A rapid detection method was developed that bypasses the laborious procedure of extracting tissue DNA samples. Instead, enzymatic digestion was used to lyse the spore wall protein present in tissue homogenates, thereby enabling direct detection of EhSW12 via antigen colloidal gold rapid test strips and dot blot assays. The colloidal gold test strips, noted for their convenience and rapidity, satisfy the requirements for on-site testing, while the dot blot method-with its simple operation-is well suited for high-throughput sample analysis. Both methods were compared with the national standard qPCR assay. Specificity was validated using various shrimp antigens prepared in the laboratory; the results demonstrated that only the EHP antigen yielded a positive reaction, with no cross-reaction observed with other common shrimp pathogens (e.g., white spot syndrome virus, decapod iridescent virus and highly pathogenic Vibrio). Sensitivity tests indicated that the minimum detection limits for the test strip and dot blot methods were, respectively, 7 × 10⁶ copies and 5.2 × 10⁴ copies. In summary, both methods exhibit strong specificity, high sensitivity, rapid detection speed and operational simplicity-characteristics that make them highly suitable for large-scale on-site rapid testing.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14160"},"PeriodicalIF":2.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary Supplementation With Ulva sp. and Garlic Extracts Enhances Growth, Immune Response and Digestive Enzyme Activity in Pacific Whiteleg Shrimp (Litopenaeus vannamei).","authors":"Nashwa Abdel-Razek, Riad H Khalil, Abeer A M Afifi, Tamer Mohammed Monir Abdelrahiem, Sameh A Metwaly, Mohamed Fathi","doi":"10.1111/jfd.70011","DOIUrl":"https://doi.org/10.1111/jfd.70011","url":null,"abstract":"<p><p>The present study investigated the effect of dietary supplementation with ethanolic extracts of Ulva sp. and garlic (Allium sativum) on the growth performance, muscle composition, digestive enzyme activity and immune response of Pacific whiteleg shrimp (Litopenaeus vannamei). A60-day feeding trial was conducted using seven experimental diets, including a control and diets supplemented with varying concentrations of Ulva sp. extract (0.5, 1.0 and 2.0 g/kg) and garlic extract (2.0, 4.0 and 6.0 g/kg). Phytochemical analysis confirmed the presence of bioactive compounds such as flavonoids and phenolics in Ulva sp. and alkaloids, saponins and phenolic acids in garlic extract. Shrimp fed Ulva sp. extract showed significant improvements in crude protein content, essential amino acid composition and digestive enzyme activity, particularly protease and lipase activities, suggesting enhanced nutrient utilisation. Conversely, garlic extract supplementation resulted in increased fat content, indicating increased fat deposition, alongside a marked upregulation ofimmune-related genes, including Penaeidin3, Penaeidin4, HSP70 and proPO, highlighting its immunostimulatory potential. The combined findings suggest that Ulva sp. extract optimally enhances protein deposition and digestive efficiency, whereas garlic extract strengthens immune responses, making both promising functional feed additives for sustainable shrimp aquaculture. Future research should explore their synergistic effects and long-term benefits in commercial farming applications.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70011"},"PeriodicalIF":2.2,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of Basic-RPA and RPA-LFD Rapid Detection Methods for Ranavirus in Largemouth Bass (Microterus salmoides).","authors":"Haoyu Wang, Yong Zhou, Jiale Zhai, Yiqun Li, Yuding Fan, Chen Peng, Jie Ma, Yan Meng, Wenzhi Liu, Mingyang Xue, Chen Xu, Zhenyu Huang, Chao Pei, Nan Jiang","doi":"10.1111/jfd.70016","DOIUrl":"https://doi.org/10.1111/jfd.70016","url":null,"abstract":"<p><p>Largemouth bass ranavirus (LMBRaV) disease causes a high mortality rate in largemouth bass aquaculture industry. Establishment of rapid and sensitive virus detection assays is an urgency for prevention of virus transmission and disease outbreak. In this study, basic recombinase polymerase amplification (basic-RPA) and RPA combined with lateral flow dipstick (RPA-LFD) assays for LMBRaV were established to detect virus at low viral loads. The mcp gene of LMBRaV was the target sequence for primers and probes design. Then the primer concentrations, reaction temperature and time of basic-RPA and RPA-LFD assays were optimised. The basic-RPA was amplified under 38°C for 25 min, the results were detected by gel electrophoresis. The RPA-LFD assay required 15 min at 45°C for on-site visual results. The specificity of two assays showed that other aquatic viruses (CyHV-2, ISKNV, GSIV, WSSV and CrERV) could not be detected. The detection limit was 1 copy/μL DNA sample for both basic-RPA and RPA-LFD assays. Additionally, basic-RPA and RPA-LFD assays both detected nine more clinic samples compared to the PCR assay. Therefore, the basic-RPA as well as RPA-LFD assays provided more sensitive, rapid and simple operation methods for the on-site diagnosis of LMBRaV.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70016"},"PeriodicalIF":2.2,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Field-Usable Lateral Flow Immunochromatographic Assay for Rapid Detection of Infectious Haematopoietic Necrosis Virus (IHNV).","authors":"Choon-Sup Kim, Kyoung-Hui Kong, Sung-Hun Lee, Wi-Sik Kim","doi":"10.1111/jfd.70014","DOIUrl":"https://doi.org/10.1111/jfd.70014","url":null,"abstract":"<p><p>A lateral flow immunochromatographic assay (LFIA) was developed for the rapid detection of infectious haematopoietic necrosis virus (IHNV, genogroups JRt-N and JRt-S) in rainbow trout (Oncorhynchus mykiss). The assay utilises the monoclonal antibody (mAb) IHNV-3, which serves as both the detector antibody in a colloidal gold-mAb conjugate and the capture antibody on the test line. The LFIA strip demonstrated a detection limit ranging from 10^5.05 to 10^5.8 TCID₅₀/100 μL for six IHNV isolates (genogroups JRt-N and JRt-S) and showed no cross-reactivity with other fish viruses. Field validation using spleen samples from 81 IHNV-infected and 102 non-infected rainbow trout revealed a specificity of 100%, a sensitivity of 97.5%, and an overall diagnostic effectiveness of 98.9%. These results highlight the LFIA strip as a reliable tool for the rapid (within 10 min) on-site detection of IHNV in rainbow trout at aquaculture facilities.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70014"},"PeriodicalIF":2.2,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High Prevalence of Silurus glanis Papillomavirus 1 (SgPV1) Detected Among Farmed Wels Catfish (Silurus glanis Linnaeus, 1758): A Retrospective Study.","authors":"András Surján, Eszter Fónagy, Boglárka Sellyei, Flóra Abonyi, Andor Doszpoly","doi":"10.1111/jfd.70018","DOIUrl":"https://doi.org/10.1111/jfd.70018","url":null,"abstract":"","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70018"},"PeriodicalIF":2.2,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Novel Fish Viruses in Wisconsin Sportfish: Implications of Co-Infections for Disease Emergence.","authors":"Charlotte E Ford, Isaac Standish, Kyle Von Ruden, Eric M Leis, Whitney A Thiel, Tony L Goldberg","doi":"10.1111/jfd.70010","DOIUrl":"https://doi.org/10.1111/jfd.70010","url":null,"abstract":"","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70010"},"PeriodicalIF":2.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Submersion Treatment of Chimeric MrN-VLPs Encapsulating Therapeutic Double-Stranded RNA Effectively Rescues Prawn Viral Infection.","authors":"Orawan Thongsum, Supawich Boonkua, Somkid Jaranathummakul, Monsicha Somrit, Charoonroj Chotwiwatthanakun, Somluk Asuvapongpatana, Pitchanee Jariyapong, Wattana Weerachatyanukul","doi":"10.1111/jfd.70009","DOIUrl":"https://doi.org/10.1111/jfd.70009","url":null,"abstract":"<p><p>One of the most successful applications to protect aquatic animals from viral infection is the use of RNA interference (RNAi) in the form of double-stranded RNA (dsRNA). However, its administration into animals is usually via injection route (laboratory scale) or mixing feed pellets with bacterial extracts (containing therapeutic compounds); the outcome of which remains to be improved. In this study, we encapsulated purified dsRNA into a virus-like particle (VLP) and delivered it into the prawn tissues by a submersion route using interiorly modified Macrobrachium rosenbergii nodavirus derived VLP with deca-arginine peptide (10R-MrN-VLP) to encapsulate a large scale (gram-level) of dsRNA against RNA2 gene of MrNV capsid. For tracing purposes of submersion administration, GFP plasmid-loaded VLP successfully delivered and expressed green fluorescent protein in prawn-targeted tissues. Delivery efficiency at 12-h submersion was about 50% of the prawn population and became 100% within 72 h, and they remained GFP-positive for up to 7 days during submersion. The protective effect of dsRNA-VLP submersion was tested with a large group (10,000) of post-larvae, which were naturally challenged with MrNV-positive dead prawn. Transcriptome analysis revealed up- and down-regulations of gene clusters involved in metabolism and signal transduction related to an antiviral immune system. The survival rate of prawn treated with dsRNA-VLP reached 80% at Day 7 post-MrNV challenge, significantly different from that of the MrNV-challenged group (control). PCR screening revealed an apparent decrease of MrNV genes and the copy number of MrNV in prawn sampling tissues. Together, we believe that the integration between the chimeric nanocontainer, RNAi technology and submersion application should be foreseen as one of the practical, powerful means to fight against prawn viral infection in field application.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e70009"},"PeriodicalIF":2.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}