Journal of fish diseases最新文献

{"title":"Alteration of Immune Function and Gut Microbiome Composition in Carassius auratus Challenged With Rahnella aquatilis.","authors":"Jiaxin Huo, Xiaowei Li, Kaiyang Zhang, Hao Fu, Xiucai Hu, Aijun Lv","doi":"10.1111/jfd.14054","DOIUrl":"https://doi.org/10.1111/jfd.14054","url":null,"abstract":"<p><p>Rahnella aquatilis as an emerging pathogen can cause bacterial enteritis in cyprinid fish such as crucian carp Carassius auratus. Currently, the characterisation of immune function and gut microbiome composition in C. auratus orally challenged by R. aquatilis were yet unknown. In this study, we therefore investigated the changes of histopathology, white blood cells (i.e., LEU, NEU and LYM), serum biochemical indicators (e.g., CRE, CK and CHO) and digestive enzyme activity (e.g., LYS, AST, ALT, GSH-Px and AKP), as well as complements and immune-related genes (e.g., C3, F2, LysC, TLR3, MyD88, TGF-β, TNF-α and IL-15) that were significantly altered after the oral administration of R. aquatilis KCL-5. Moreover, the gut microbiome composition and diversity were analysed by using 16S rRNA gene high-throughput sequencing analysis. The correlation analysis showed that the high abundance of phylum Proteobacteria, Actinobacteria, Firmicutes and Fusobacteria was related to the pathogenesis of enteritis caused by oral infection. KEGG enrichment analysis indicated that fatty acid, carbon and pyruvate metabolism were significantly increased pathways (p < 0.05). To our best knowledge, this is a rare report of physicochemical properties and gut microbiome in C. auratus by R. aquatilis infection, which will provide a scientific reference for the clinical diagnosis and prevention of bacterial enteritis in cyprinid fish.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14054"},"PeriodicalIF":2.2,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial Activity and Action Mechanism of Synthetic Interleukin-8 Derived Peptides Against Flavobacterium psychrophilum.","authors":"Paula A Santana, Laura Tamayo, Felipe Stambuk, Luis Felipe Aguilar, Marcos Cortés, Fanny Guzmán, Juan Carlos Forero, María Soledad Romero, Claudio A Álvarez","doi":"10.1111/jfd.14056","DOIUrl":"https://doi.org/10.1111/jfd.14056","url":null,"abstract":"<p><p>In Chile, Atlantic salmon and rainbow trout face significant production challenges due to the presence of Flavobacterium psychrophilum, which generates severe disease issues and economic losses. To address this, the salmon industry relies on vaccines and antibiotics, the latter raising concerns about bacterial resistance. For that reason, our study explores an alternative strategy for controlling F. psychrophilum infections based on host defence peptides. We previously identified and characterised IL-8-derived salmonid peptides (ssIL-8α and omIL-8α) with potential antimicrobial properties. In the current study, we further investigated the antibacterial activity and mechanism of action of these peptides against F. psychrophilum. First, we demonstrated the antibacterial activity of ssIL-8α and omIL-8α synthetic peptides. Then we evaluated the effects of these peptides on membrane fluidity and localisation on bacterial cells by fluorescence microscopy as well as its impact on bacterial morphology and ultrastructure by electron microscopy. The results indicate that the ssIL-8α at 30 μM exhibits superior efficacy in inhibiting the growth of F. psychrophilum. Also, both ssIL-8α and omIL-8α can bind to pathogen membrane, but ssIL-8α exhibits a higher binding capacity compared to omIL-8α against F. psychrophilum. omIL-8α exhibited the ability to induce early membrane alterations within 15 min, at concentrations of 15 or 30 μM. The SEM and TEM micrographs showed membrane disruption of the bacteria after incubation with ssIL-8α or omIL-8α. However, the damage was more pronounced in the ssIL-8α treatment, as evidenced by a complete detachment of the outer membrane after a 20-min exposure of F. psychrophilum. This study reveals that these peptides significantly alter bacterial membrane morphology, leading to bacterial death, highlighting their potential as alternative treatments in flavobacterial disease control. This work contributes to understanding host defence peptides' role in combating bacterial infections and reducing antibiotic resistance in aquaculture.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14056"},"PeriodicalIF":2.2,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Case of 'Soft Flesh' Induced by Kudoa thyrsites in an Atlantic Bonito (Sarda sarda).","authors":"Felice Panebianco, Stefano Bagatella, Tiziana Civera, Selene Rubiola","doi":"10.1111/jfd.14053","DOIUrl":"https://doi.org/10.1111/jfd.14053","url":null,"abstract":"","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14053"},"PeriodicalIF":2.2,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological, Histological, and Phylogenetic Characterization of Myxobolus branchioepidermis n. sp. (Myxosporea: Myxobolidae) Infecting the Gills and Skin of the Oranda Goldfish (Carassius auratus).","authors":"Hooman Rahmati-Holasoo, Amin Marandi, Hosseinali Ebrahimzadeh Mousavi, Sara Shokrpoor, Fatemeh Arabkhazaeli, Zahra Ziafati Kafi","doi":"10.1111/jfd.14040","DOIUrl":"https://doi.org/10.1111/jfd.14040","url":null,"abstract":"<p><p>Myxobolus Bütschli, 1882 is a highly important genus of fish myxozoans, consisting of many species that cause serious diseases in ornamental cyprinid fish species in different geographic areas by infecting a wide range of organs. This study describes a new Myxobolus species infecting the gill lamellae, gill rakers and skin epidermis of goldfish (Carassius auratus) from Mohammadshahr, Iran, based on detailed morphological, histological and molecular analysis. Mature spores were small and spherical in shape, consisting of two symmetrical shell valves in frontal view, measuring 10.3 (9.8-10.9) μm in length and 10.5 (10.3-11.2) μm in width. Two equal-sized relatively pyriform polar capsules were 5.4 (3.9-6.0) μm long and 4.5 (3.7-5.2) μm wide. The polar tubules were coiled with 4-6 turns. A comparative analysis of the morphological and molecular data confirmed that this species is distinct from other identified myxosporeans. Plasmodia in the cutaneous tissue were found in the epidermis, causing extensive hyperplasia of Malpighian cells and mild inflammatory infiltration. In the gill tissue, plasmodia affected only one side of the lamellae, leading to hyperplasia of the lamellar epithelium, edema, and lymphoplasmacytic branchitis. A novel myxozoan species, Myxobolus branchioepidermis n. sp., has been proposed. The study provides a comprehensive analysis of the morphological, histological, and molecular characteristics of this parasite, which infects the gill lamellae and epidermal layer of the skin in oranda goldfish (C. auratus). The identification of this new species adds to the growing body of research on myxozoan parasites and highlights its potential impact on ornamental fish aquaculture.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14040"},"PeriodicalIF":2.2,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Co-Infection of ISKNV-II and RGNNV Resulting in Mass Mortality of Juvenile Asian Seabass (Lates calcarifer), Zhuhai, Southern China.","authors":"Qianqian Sun, Shan Ding, Xiangrong Liu, Wenfeng Zhang, Fangzhao Yu, Yuting Fu, Yong Li, Chuanfu Dong","doi":"10.1111/jfd.14052","DOIUrl":"https://doi.org/10.1111/jfd.14052","url":null,"abstract":"<p><p>Infectious spleen and kidney necrosis virus (ISKNV) and nervous necrosis virus (NNV) are two common and important causative agents in marine-cultured fish. However, high viral loads of both ISKNV and NNV in the same clinical case is unusual. In this study, a mass mortality event of Asian seabass Lates calcarifer juveniles occurred in Zhuhai, the main Asian seabass cultured area in mainland China. The fish samples were pooled for pathogen identification and both high viral loads of ISKNV and NNV were detected by real-time microfluidic quantitative PCR and conventional PCR. Immunohistochemistry and immunofluorescence showed that strong ISKNV signals were detected in spleen and liver, while strong NNV signals were detected in brain and eye. The tissue homogenates were inoculated into MFF-1 cell and SSN-1 cell, respectively. After several viral passages, both individual ISKNV and NNV were purely isolated from each other, and designated as ASB-ISKNV-23 and ASB-NNV-23, respectively. The whole genome sequences of ASB-ISKNV-23 and ASB-NNV-23 were determined and annotated. The result showed that ASB-ISKNV-23 and ASB-NNV-23 are composed of 112,236 bp and 4441 bp, respectively. Phylogeny analysis showed that ASB-ISKNV-23 belongs to ISKNV-II sub-genotype and ASB-NNV-23 belongs to RGNNV genotype. Collectedly, coinfection of ISKNV-II and RGNNV were firstly documented in mass mortality of Asian seabass in mainland China. Importantly, both individual ISKNV-II and RGNNV were purely isolated using two different permissive cell lines. Our study provides useful information for better understanding the complex pathogenesis regarding the coinfection with ISKNV and NNV in farmed fish.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14052"},"PeriodicalIF":2.2,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial Susceptibility and Local Epidemiological Cut-Off Values of Vibrio anguillarum Isolated From Fish Farms in Turkey","authors":"Muhammed Duman,&nbsp;Soner Altun,&nbsp;Izzet Burçin Satıcıoğlu,&nbsp;Kübra Balcı,&nbsp;Merve Taşgın,&nbsp;Nisa Bayrak,&nbsp;Christopher Concha,&nbsp;Claudio D. Miranda,&nbsp;Ruben Avendaño-Herrera","doi":"10.1111/jfd.14049","DOIUrl":"10.1111/jfd.14049","url":null,"abstract":"<div>\u0000 \u0000 <p>Studies on preventing antimicrobial resistance in aquaculture emphasise the need to responsibly and prudently use antimicrobials, selecting those most effective in controlling and/or reducing mortalities caused by vibriosis. In this study, the distribution of the antimicrobial susceptibility of 28 <i>Vibrio anguillarum</i> isolates from Turkish fish farms was determined using the broth microdilution minimum inhibitory concentration (MIC) test according to the Clinical and Laboratory Standards Institute (CLSI) guideline. The epidemiological cut-off (CO_WT) values of the <i>V. anguillarum</i> isolates of florfenicol (FLO), tetracycline (TET), doxycycline (DOX), oxolinic acid (OXO), enrofloxacin (ENR) and trimethoprim/sulfamethoxazole (SXT) were calculated using the normalised resistance interpretation (NRI) and ECOFFinder methods. Isolates were categorised as belonging to the fully susceptible wild-type (WT) or non-wild-type (NWT) populations. Calculated CO_WT values (in μg mL⁻¹) were ≤ 1.0 for FLO and DOX, ≤ 0.5 for TET, ≤ 0.016 for ENR, ≤ 0.032 for OXO, and ≤ 4.0 for SXT using the NRI analysis. Percentages of <i>V. anguillarum</i> isolates categorised as belonging to the NWT population were small for the antimicrobials FLO (10.7%) and SXT (0%), whereas they were higher for the antimicrobials OXO (39.3%) and ENR (39.3%). This is the first study to estimate the local CO_WT values for antibiotics used in the control of <i>V. anguillarum</i> isolates recovered from farmed fish in Turkey.</p>\u0000 </div>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":"48 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142750995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Binding of Infectious Hypodermal and Haematopoietic Necrosis Virus-Like Particles to Mannose Receptor Stimulates Antimicrobial Responses in Immune-Related Tissues of Peneaus vannamei","authors":"Wattana Weerachatyanukul,&nbsp;Chettupon Pooljun,&nbsp;Charoonroj Chotwiwatthanakun,&nbsp;Pitchanee Jariyapong","doi":"10.1111/jfd.14051","DOIUrl":"10.1111/jfd.14051","url":null,"abstract":"<div>\u0000 \u0000 <p>Mannose receptor (MR) is a transmembrane protein and a type of pattern-recognition receptor (PRR) that plays a critical role in the immunity of mammals and fish. In this study, we examined the role of MR in binding with infectious hypodermal and haematopoietic necrosis virus-like particle (IHHN-VLP) and the downstream immune pathway that it triggers in the shrimp <i>Peneaus vannamei</i>. Upon IHHN-VLP challenge, transcripts of MR in <i>P. vannamei</i> (<i>Pv</i>MR) increased significantly in all examined tissues, particularly those related to shrimp immunity, including hemocyte, hepatopancreas and gill tissues. Specifically, IHHN-VLP bound to the 34-kDa <i>Pv</i>MR protein in shrimp-tissue extracts. Immunohistochemistry results of hemocytes showed that <i>Pv</i>MR was initially localised on the plasma membrane but later internalised and dispersed throughout the cytoplasm after IHHN-VLP administration. Binding between IHHN-VLP and <i>Pv</i>MR also induced significant upregulation of genes for the antimicrobial peptides (AMPs) penaeidin 3 and crustin, presumably to protect the shrimp against the viral infection. However, knocking down <i>Pv</i>MR resulted in down-regulation of all immune-related genes examined. Overall, as an immune-related PRR, <i>Pv</i>MR serves as a receptor for invading viruses, which then trigger the expression of AMPs. Strategic designs using <i>Pv</i>MR could be developed to either block the interaction of native virus with the host cells or provoke its up-regulation to enhance shrimp immunity, which could open up opportunities to fight against IHHNV infection in shrimp.</p>\u0000 </div>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":"48 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142751005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological Study of Parasites of the Edible Scallop Aequipecten tehuelchus in Patagonia, Southwest Atlantic Ocean","authors":"Nuria Vázquez,&nbsp;Carmen Gilardoni,&nbsp;Florencia Cremonte","doi":"10.1111/jfd.14047","DOIUrl":"10.1111/jfd.14047","url":null,"abstract":"<div>\u0000 \u0000 <p>This study assesses the health status of the edible Tehuelche scallop, <i>Aequipecten tehuelchus</i>, reporting the epidemiological results of the parasites and their infection levels by histopathological analysis and transmission electron microscopy. Environmental factors (site: West and East domains of the San José gulf, seawater temperature) and host traits (size, physiological condition index, gonad development stages) associated with parasite load (prevalence, mean intensity, parasite abundance and total parasite abundance) were tested with generalised linear models (GLMs). Histopathological results revealed <i>Rickettsia</i>-like organisms (RLOs), several protozoans (<i>Trichodina</i> sp., <i>Nematopsis</i> sp. and APXSc apicomplexans) and metazoans (turbellarians and <i>Tumidotheres maculatus</i> pea crabs) as well as inflammatory lesions in form of granulomas and hemocytic infiltration. The prevalence of the parasitofauna did not vary significantly between gulf domains, except the apicomplexan APXSc that was significantly most prevalent in scallops from the East Domain. However, the mean intensity of infection seemed to be determined by the gulf domains, which present distinct hydrographic regimes, where RLO's, <i>Trichodina</i> sp. and <i>T. maculatus</i> were significantly more intense in scallops from the West, opposite with <i>Nematopsis</i> sp. infection. Total parasite abundance was influenced by seasonal variations, it was negatively correlated with warmer temperatures. The physiological condition index of <i>A. tehuelchus</i> seemed not to be affected by the presence or abundance of any of the parasites. Nevertheless, the apicomplexan APXSc would represent a significant risk to the health of <i>A. tehuelchus</i> due to the pathological conditions, and surveillance of this parasite is needed for a sustainable <i>A. tehuelchus</i> fishery.</p>\u0000 </div>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":"48 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142751008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Professor Ronald John Roberts, CCT, FRCVS, FRCPath, FRSE, (b) 1941, (q) Glasgow 1964, Died 3rd August 2024.","authors":"Hamish Rodger","doi":"10.1111/jfd.14041","DOIUrl":"https://doi.org/10.1111/jfd.14041","url":null,"abstract":"","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14041"},"PeriodicalIF":2.2,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142716114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temperature-Related Effects on Disease Susceptibility and Immune Response in Redband Trout (Oncorhynchus mykiss gairdneri) Following Challenge With Flavobacterium columnare","authors":"Joshua P. Egan,&nbsp;Jie Ma,&nbsp;Veronica L. Myrsell,&nbsp;Zhongqi Chen,&nbsp;Jonathan Masingale,&nbsp;Christopher C. Caudill,&nbsp;Timothy Boyle,&nbsp;Julianna Browning,&nbsp;Shawn R. Narum,&nbsp;Kenneth D. Cain,&nbsp;Paul A. Hohenlohe","doi":"10.1111/jfd.14046","DOIUrl":"10.1111/jfd.14046","url":null,"abstract":"<div>\u0000 \u0000 <p>Heat stress can increase disease risk in fishes by reducing immune function. Interactions between redband trout (<i>Oncorhynchus mykiss gairdneri</i>) and <i>Flavobacterium columnare</i>, a causative agent of columnaris disease, provide an opportunity to investigate the effects of temperature on immune function and disease resistance during periods of thermal stress. We conducted three trials to characterise differences in immune function and mortality between redband trout held at 18°C and 21°C following challenge with <i>F</i>. <i>columnare</i>. In trial 1, cumulative per cent mortality (CPM) was low and not statistically different between 18°C and 21°C. In trials 2 and 2, we administered higher challenge doses and observed increased CPM overall and significantly greater CPM at 21°C than 18°C. Redband trout upregulated <i>il-8</i>, <i>tnf-α</i>, <i>igm</i> and <i>igt</i> following infection by <i>F</i>. <i>columnare</i>, suggesting that all of these genes may be involved in immune responses to <i>F</i>. <i>columnare</i> infection. We found no differences in the strength of the immune responses between fish held at 21°C versus 18°C. This indicated that 21°C did not elicit sufficient thermal stress to impair immune function and that increased CPM at 21°C versus 18°C was due to enhanced <i>F</i>. <i>columnare</i> virulence.</p>\u0000 </div>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":"48 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}