Journal of fish diseases最新文献_第4页

Ichthyobodo Infection Status of Juvenile Chum Salmon in the Chitose River, Japan, During Stocking. 日本千岁河鲑鱼幼鱼放养期间鱼蛆病感染状况。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-03-27 DOI: 10.1111/jfd.14124

Yuhei Ogura

引用次数: 0

Comparisons of Hyperostotic Bones in Two Species of the Genus Oligoplites Gill, 1863 in the Southern Brazilian Coast. 1863年在巴西南部海岸发现的Oligoplites Gill属两种骨增厚骨的比较。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-03-27 DOI: 10.1111/jfd.14117

Fernando A P Tuna, Ana Paula C de Rodrigues, Gustavo P Filgueira, Rebeca S da Bianca, Wilson T V Machado, Márcia C Salomão, Helena P Lavrado

{"title":"Comparisons of Hyperostotic Bones in Two Species of the Genus Oligoplites Gill, 1863 in the Southern Brazilian Coast.","authors":"Fernando A P Tuna, Ana Paula C de Rodrigues, Gustavo P Filgueira, Rebeca S da Bianca, Wilson T V Machado, Márcia C Salomão, Helena P Lavrado","doi":"10.1111/jfd.14117","DOIUrl":"https://doi.org/10.1111/jfd.14117","url":null,"abstract":"The description of skeletal disorders is commonly reported in fish species raised through aquaculture; however, wild marine fish may also exhibit high frequencies of anomalies, particularly the development of hyperostosis, a condition that causes thickening of the bones. Specimens of Oligoplites saliens (n = 15) and Oligoplites palometa (n = 14) were radiographed to verify hyperostotic bone occurrences. Hyperostosis was detected in all individuals (> 400 mm TL) analysed for both species, occurring in six distinct regions of the skeleton (supraoccipital bone, cleithrum, dorsal pterygiophores, anal pterygiophore, haemal spines, and neural spines), regardless of the sex of the individuals. No hyperostosis was found in the neural and haemal spines of O. palometa and in the anal pterygiophore of O. saliens. Histological sections of the supraoccipital bone of O. saliens were conducted, revealing high porosity characterised by the presence of vascular channels in bones with hyperostosis. The species exhibited species-specific patterns for the condition, demonstrating differences even among species of the same genus occurring in the same geographic area. This is the first record of hyperostosis for both species in Brazilian waters.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14117"},"PeriodicalIF":2.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum and Tissue Kinetics of Oxolinic Acid in Black Rockfish (Sebastes schlegelii) Following a Single Oral Administration at Two Temperatures. 两种温度下单次口服黑岩鱼（sebases schlegelii）体内氧喹啉酸的血清和组织动力学。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-03-26 DOI: 10.1111/jfd.14116

Jun Sung Bae, Chae Won Lee, Chan Yeong Yang, Eun Ha Jeong, Jung Soo Seo, Mun-Gyeong Kwon, Ji-Hoon Lee

{"title":"Serum and Tissue Kinetics of Oxolinic Acid in Black Rockfish (Sebastes schlegelii) Following a Single Oral Administration at Two Temperatures.","authors":"Jun Sung Bae, Chae Won Lee, Chan Yeong Yang, Eun Ha Jeong, Jung Soo Seo, Mun-Gyeong Kwon, Ji-Hoon Lee","doi":"10.1111/jfd.14116","DOIUrl":"https://doi.org/10.1111/jfd.14116","url":null,"abstract":"The objective of this study was to investigate the pharmacokinetic (PK) properties of oxolinic acid (OA) in black rockfish (Sebastes schlegelii) following a single oral administration of 30 mg/kg at 13°C and 22°C. Serum and tissue (muscle, liver and kidney) samples were collected at pre-determined time points and analysed using high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS). The results demonstrated that temperature significantly influenced the PK parameters of OA. The terminal half-life (t1/2λz) was prolonged at 22°C across all tissues, while the total body clearance (CL/F) was reduced, suggesting a slower drug elimination at higher temperatures. The peak concentration (Cmax) and the area under the concentration-time curve (AUC) values increased with temperature, indicating enhanced systemic exposure at 22°C. The volume of distribution (Vz/F) decreased from 13°C to 22°C, indicating reduced tissue distribution of OA at the higher temperature. PK/pharmacodynamic (PK/PD) analysis revealed that a single oral dose of 30 mg/kg was insufficient to achieve optimal antibacterial efficacy, implying that higher doses may be necessary. These findings highlight the necessity of temperature-adjusted dosing strategies for OA treatment in black rockfish aquaculture to optimise therapeutic efficacy while minimising antimicrobial resistance risks.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14116"},"PeriodicalIF":2.2,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic and Genotypic Antimicrobial Resistance Profiles of Flavobacterium psychrophilum and Flavobacterium branchiophilum Isolated From Rainbow Trout (Oncorhynchus mykiss) in Slovenia. 斯洛文尼亚虹鳟（Oncorhynchus mykiss）嗜冷黄杆菌和嗜支黄杆菌的表型和基因型耐药性分析

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-03-24 DOI: 10.1111/jfd.14119

Katarina Pavlin, Bojan Papić, Irena Zdovc, Tanja Knific, Igor Gruntar, Rosvita Sitar, Diana Žele Vengušt, Marija Seničar, Matjaž Ocepek, Tanja Švara

{"title":"Phenotypic and Genotypic Antimicrobial Resistance Profiles of Flavobacterium psychrophilum and Flavobacterium branchiophilum Isolated From Rainbow Trout (Oncorhynchus mykiss) in Slovenia.","authors":"Katarina Pavlin, Bojan Papić, Irena Zdovc, Tanja Knific, Igor Gruntar, Rosvita Sitar, Diana Žele Vengušt, Marija Seničar, Matjaž Ocepek, Tanja Švara","doi":"10.1111/jfd.14119","DOIUrl":"https://doi.org/10.1111/jfd.14119","url":null,"abstract":"Flavobacterium psychrophilum and Flavobacterium branchiophilum are important fish pathogens that cause considerable economic losses in freshwater aquaculture worldwide. Their antimicrobial resistance (AMR) profiles were evaluated using the latest Clinical and Laboratory Standards Institute broth microdilution method, which was adjusted for F. branchiophilum to achieve growth, and whole-genome sequencing (WGS). A total of 51 F. psychrophilum isolates and eight F. branchiophilum isolates from Slovenian farmed rainbow trout underwent phenotypic antimicrobial susceptibility testing. Overall, 86.3% of F. psychrophilum isolates were classified as non-wild-type (NWT) for oxytetracycline and enrofloxacin, and 90.2% of isolates were NWT for oxolinic acid. In contrast, all isolates tested were classified as wild-type (WT) for florfenicol and erythromycin. It was not possible to classify F. branchiophilum isolates as WT or NWT for the antimicrobials tested. A subset of F. psychrophilum (n = 7) and F. branchiophilum (n = 2) isolates was further characterised using WGS to investigate the mechanisms mediating antimicrobial resistance. In F. psychrophilum, the T83A or T83V substitution in GyrA was associated with reduced susceptibility to oxolinic acid. No other AMR genes or AMR-associated mutations were detected.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14119"},"PeriodicalIF":2.2,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Survey on Kudoa thyrsites in Sardina pilchardus From the Central Tyrrhenian Sea. 中第勒尼安海撒丁岛沙丁鱼中库多亚丝蕊西斯的调查。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-03-24 DOI: 10.1111/jfd.14123

Paolo Cipriani, Marialetizia Palomba, Luca Ruggiero, Stig Mæle, Lucilla Giulietti

引用次数: 0

A Review of In Silico Approaches for Discovering Natural Viral Protein Inhibitors in Aquaculture Disease Control. 在水产养殖疾病控制中发现天然病毒蛋白抑制剂的计算机方法综述。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-03-20 DOI: 10.1111/jfd.14120

Luu Tang Phuc Khang, Nguyen Dinh-Hung, Sk Injamamul Islam, Sefti Heza Dwinanti, Samuel Mwakisha Mwamburi, Patima Permpoonpattana, Nguyen Vu Linh

{"title":"A Review of In Silico Approaches for Discovering Natural Viral Protein Inhibitors in Aquaculture Disease Control.","authors":"Luu Tang Phuc Khang, Nguyen Dinh-Hung, Sk Injamamul Islam, Sefti Heza Dwinanti, Samuel Mwakisha Mwamburi, Patima Permpoonpattana, Nguyen Vu Linh","doi":"10.1111/jfd.14120","DOIUrl":"https://doi.org/10.1111/jfd.14120","url":null,"abstract":"Viral diseases pose a significant threat to the sustainability of global aquaculture, causing economic losses and compromising food security. Traditional control methods often demonstrate limited effectiveness, highlighting the need for alternative approaches. The integration of computational methods for the discovery of natural compounds shows promise in developing antiviral treatments. This review critically explores how both traditional and advanced in silico computational techniques can efficiently identify natural compounds with potential inhibitory effects on key pathogenic proteins in major aquaculture pathogens. It highlights fundamental approaches, including structure-based and ligand-based drug design, high-throughput virtual screening, molecular docking, and absorption, distribution, metabolism, excretion and toxicity (ADMET) profiling. Molecular dynamics simulations can serve as a comprehensive framework for understanding the molecular interactions and stability of candidate drugs in an in silico approach, reducing the need for extensive wet-lab experiments and providing valuable insights for targeted therapeutic development. The review covers the entire process, from the initial computational screening of promising candidates to their subsequent experimental validation. It also proposes integrating computational tools with traditional screening methods to enhance the efficiency of antiviral drug discovery in aquaculture. Finally, we explore future perspectives, particularly the potential of artificial intelligence and multi-omics approaches. These innovative technologies can significantly accelerate the identification and optimisation of natural antivirals, contributing to sustainable disease management in aquaculture.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14120"},"PeriodicalIF":2.2,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Malignant Mixed Irido-Melanophoroma in a Betta splendens with Concurrent Mycobacterium chelonae Isolation-A Case Report. 伴有龟分枝杆菌分离的锦鲤恶性混合型虹膜黑色素瘤1例。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-03-19 DOI: 10.1111/jfd.14115

Zi An Yu, Lorraine A Corriveau, Joanne B Messick

引用次数: 0

Rapid Detection of Aeromonas veronii Using GGDEF Gene-Based Colorimetric Loop-Mediated Isothermal Amplification. 基于GGDEF基因的比色环介导等温扩增快速检测维罗氏气单胞菌。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-03-11 DOI: 10.1111/jfd.14112

Tien Tien Vicky Lau, Suat Moi Puah, Kek Heng Chua

{"title":"Rapid Detection of Aeromonas veronii Using GGDEF Gene-Based Colorimetric Loop-Mediated Isothermal Amplification.","authors":"Tien Tien Vicky Lau, Suat Moi Puah, Kek Heng Chua","doi":"10.1111/jfd.14112","DOIUrl":"https://doi.org/10.1111/jfd.14112","url":null,"abstract":"Aeromonas veronii has emerged as an important fish pathogen that poses threats to the aquaculture industry worldwide, and rapid detection is essential to minimise negative economic impact. In this study, a colorimetric loop-mediated isothermal amplification (LAMP) assay was developed to rapidly detect A. veronii, targeting a gene encoding GGDEF domain-containing diguanylate cyclase. The entire LAMP reaction could be completed in 30 min at 65°C based on colour change from pink to yellow. In addition, the assay demonstrated 100% specificity with no cross reaction with other common fish pathogens. The detection limit (LOD) was 500 pg/μL using purified plasmid and 1.72 × 105 cfu/mL (equivalent to 1.72 × 102 cfu/reaction) using crude genomic DNA extracted from the pure culture of A. veronii ATCC 9071. LAMP demonstrated comparable performance to conventional PCR using 57 bacterial isolates, with 100% (20/20) specificity, 91.9% (34/37) sensitivity, 94.7% (54/57) accuracy and a 0.888 Cohen's kappa value. Lastly, the LOD of LAMP in a spiked water sample was 1.72 × 105 cfu/mL (equivalent to 3.44 × 103 cfu/reaction). Overall, our LAMP assay has a high level of diagnostic agreement with conventional PCR and can be used as a valuable tool for the rapid detection of A. veronii from environmental samples.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14112"},"PeriodicalIF":2.2,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of Biomarkers in Plasma and Faeces to Evaluate Physiological Status of Atlantic Salmon (Salmo salar L.) During Salmonid Alphavirus Infection. 应用血浆和粪便生物标志物评价大西洋大马哈鱼生理状态在感染沙门氏病毒期间。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-03-05 DOI: 10.1111/jfd.14114

Eirik Hoel, Frederike Keitel-Gröner, Christina Aspaas Husebø, David Lausten Knudsen, Julia Mullins, Christian Wallace, Hanne Log Persson, Hanna S Bjerke-Ertenstein, Maren Iden Bjerkeset, Kjetil Berge

{"title":"Use of Biomarkers in Plasma and Faeces to Evaluate Physiological Status of Atlantic Salmon (Salmo salar L.) During Salmonid Alphavirus Infection.","authors":"Eirik Hoel, Frederike Keitel-Gröner, Christina Aspaas Husebø, David Lausten Knudsen, Julia Mullins, Christian Wallace, Hanne Log Persson, Hanna S Bjerke-Ertenstein, Maren Iden Bjerkeset, Kjetil Berge","doi":"10.1111/jfd.14114","DOIUrl":"https://doi.org/10.1111/jfd.14114","url":null,"abstract":"Salmonid alphavirus (SAV) is the causative agent of pancreas disease (PD), a disease that can cause severe implications for marine farming of Atlantic salmon. This study examines physiological changes in Atlantic salmon during SAV infection through a controlled trial and two field trials. In the controlled trial, plasma creatine kinase (CK), alanine aminotransferase (ALAT) and aspartate aminotransferase (ASAT) levels increased significantly 4 weeks post challenge, peaked at 8 weeks and by 12 weeks, ALAT levels returned to pre-challenge levels, while CK and ASAT remained elevated. Weekly mortality peaked at 4.1% in week 5, and there was an 89% reduction in appetite in week 4 post challenge. In two field trials in southern Norway, SAV3 was detected in spring/early summer by reverse transcription quantitative polymerase chain reaction (RT-qPCR). As SAV prevalence increased, plasma CK, ALAT, ASAT and lactate dehydrogenase (LDH) levels peaked, along with the stress markers cortisol and tetrahydrocortisone in faeces. Despite low mortality (< 0.2% weekly), a decrease in appetite was observed in both field trials. Understanding the physiological status of the fish through monitoring tools like RT-qPCR, blood chemistry and stress markers provides a foundation for implementing mitigation measures such as stress reduction, timed harvest or clinical nutrition at the onset of pancreas disease.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14114"},"PeriodicalIF":2.2,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and Pathogenicity of Lactococcus Species in Nile Tilapia (Oreochromis niloticus) and Asian Sea Bass (Lates calcarifer). 尼罗罗非鱼（Oreochromis niloticus）和亚洲黑鲈（Lates calcarifer）乳球菌的鉴定及致病性。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2025-03-04 DOI: 10.1111/jfd.14113

Janchai Wongkaew, Satid Chatchaiphan, Suwimon Taengphu, Ha Thanh Dong, Saengchan Senapin, Sanit Piyapattanakorn

{"title":"Identification and Pathogenicity of Lactococcus Species in Nile Tilapia (Oreochromis niloticus) and Asian Sea Bass (Lates calcarifer).","authors":"Janchai Wongkaew, Satid Chatchaiphan, Suwimon Taengphu, Ha Thanh Dong, Saengchan Senapin, Sanit Piyapattanakorn","doi":"10.1111/jfd.14113","DOIUrl":"https://doi.org/10.1111/jfd.14113","url":null,"abstract":"Lactococcosis, primarily caused by Lactococcus garvieae, is prevalent in various freshwater fish species globally. Yet, its significance in Thai fish aquaculture remains poorly understood. This study investigated Lactococcosis-associated diseases across four red tilapia (Oreochromis sp.) and one Asian sea bass (Lates calcarifer) farm in Thailand in 2022. Chronically diseased fish displaying clinical signs such as exophthalmia or corneal opacity, erratic swimming, scale loss and skin haemorrhage, as well as apparently healthy specimens, were studied. Bacterial isolation was performed from the fish internal organs. Gram-positive bacterial isolates were selected and later identified as L. lactis (n = 4), L. taiwanensis (n = 1), L. garvieae (n = 2) and L. petauri (n = 2) based on bacterial 16S rDNA and gyrB partial sequences. Sub-adults of Nile tilapia were intracoelomic (IC) injected with representatives of each of the three Lactococcus species (L. garvieae, L. lactis and L. taiwanensis) at varying doses (103-107 CFU/fish), while juvenile Asian sea bass were IC injected with L. petauri at doses ranging from 102 to 108 CFU/fish. Control groups received culture medium alone. By the end of the 21-day test, mortality rates from all challenge groups ranged from 0% to 14.82%. Some clinical signs were observed in groups challenged with higher doses of the bacteria. Enzyme-linked immunosorbent assay (ELISA) showed that survivor fish exposed to high bacterial doses elicited specific antibodies IgM. In summary, this study identifies the presence of Lactococcus species in farmed fishes, which exhibit low virulence in Nile tilapia and Asian sea bass under laboratory-controlled challenges. These bacterial isolates appear to be opportunistic rather than pathogenic, potentially indicating underlying health challenges within the affected farms.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14113"},"PeriodicalIF":2.2,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0