脾脏作为评估褐鳟四类苔藓菌负荷、流行率及其与增生性肾脏疾病关系的替代组织。

IF 2.2 3区 农林科学 Q2 FISHERIES
Magnus Lauringson, Lilian Pukk, Siim Kahar, Oksana Burimski, Riho Gross, Veljo Kisand, Anti Vasemägi
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引用次数: 0

摘要

准确的病原体检测对鱼类健康管理和疾病预防至关重要。病原体通常针对不同的宿主组织,监测不同的靶器官可以为疾病进展提供重要的见解。我们评估了脾脏作为肾脏的替代品,以估计粘虫寄生虫brysalmonae (Tb)的负荷和流行及其与幼年褐鳟增生性肾脏疾病(PKD)的关系。我们在9条河流中采集了238条褐鳟样本,并使用qPCR定量了两种组织中的寄生虫负荷。在原始数据和log10转换数据中,两种组织之间的寄生虫负荷都显示出很强的正相关(Pearson’s r = 0.60-0.71, Spearman’s ρ = 0.78),脾脏的寄生虫负荷平均比肾脏低4.1倍。191例标本中发现结核,其中脾167例,肾190例。在两种组织中,寄生虫负荷与PKD症状(肾增生和贫血)之间的关系具有可比性,分段回归线分析表明,超过一定寄生虫负荷阈值后,健康参数恶化得更快。总之,这些结果表明,脾脏可以作为一种可行的替代肾脏进行结核病监测,为沙门氏菌中结核病的存在、负荷和PKD进展提供有用的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Spleen as an Alternative Tissue for Estimating Tetracapsuloides bryosalmonae Load, Prevalence and Its Relationship With Proliferative Kidney Disease in Brown Trout.

Accurate pathogen detection is essential in fish health management and disease prevention. Pathogens often target different host tissues, and monitoring alternative target organs can provide important insights into disease progression. We evaluated the spleen as an alternative to the kidney for estimating the load and prevalence of the myxozoan parasite Tetracapsuloides bryosalmonae (Tb) and its relationships with proliferative kidney disease (PKD) in juvenile brown trout. We sampled 238 brown trout across nine rivers and quantified parasite load in both tissues using qPCR. Parasite load showed a strong positive correlation between the two tissues for both raw and log10-transformed data (Pearson's r = 0.60-0.71, Spearman's ρ = 0.78), with the spleen exhibiting, on average, 4.1-fold lower parasite load compared to the kidney. Tb was found in 191 specimens, consisting of 167 spleen and 190 kidney detections. The relationships between parasite load and PKD symptoms (renal hyperplasia and anaemia) were comparable for both tissues, and segmented regression line analysis indicated that health parameters deteriorate faster after exceeding a certain parasite load threshold. In conclusion, these results suggest that the spleen may serve as a viable alternative to the kidney for Tb monitoring, providing useful insights into Tb presence, load, and PKD progression in salmonids.

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来源期刊
Journal of fish diseases
Journal of fish diseases 农林科学-海洋与淡水生物学
CiteScore
4.60
自引率
12.00%
发文量
170
审稿时长
6 months
期刊介绍: Journal of Fish Diseases enjoys an international reputation as the medium for the exchange of information on original research into all aspects of disease in both wild and cultured fish and shellfish. Areas of interest regularly covered by the journal include: -host-pathogen relationships- studies of fish pathogens- pathophysiology- diagnostic methods- therapy- epidemiology- descriptions of new diseases
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