Journal of General and Applied Microbiology最新文献_第7页

Structure and Functional Potential of Arctic Sea Sediment Microbiota. 北冰洋沉积物微生物群的结构和功能潜力。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2023-06-22 DOI: 10.2323/jgam.2022.10.001

William Kurdy, Galina Yakovleva, Olga Ilinskaya

{"title":"Structure and Functional Potential of Arctic Sea Sediment Microbiota.","authors":"William Kurdy, Galina Yakovleva, Olga Ilinskaya","doi":"10.2323/jgam.2022.10.001","DOIUrl":"https://doi.org/10.2323/jgam.2022.10.001","url":null,"abstract":"Arctic ecosystems are affected by negative influence of climate change, pollution, and overexploitation of resources. Microorganisms playing a key role in preserving extreme econiches are poorly studied and require the use of modern methods for studying both their biodiversity and physiological activity. We applied Illumina MiSeq to the high-throughput 16S rRNA sequencing study of four Laptev Sea sediments from 64 - 185 m depth, using next generation sequencing enables rapid analysis of composition and diversity of prokaryotic communities. Although the dominant phylum in all samples was Proteobacteria, only the deepest sample contained a high number of archaeal organisms (19%) with the predominance of Methanosarcinaceace family in comparison with less 1% in the other three samples. This deepest sample had the lowest biodiversity and richness indices. Comparison of functional profiles of communities using Global Mapper tool revealed similar average abundance of infectiousness, drug resistance and environmental adaptation determinants in all samples, and high functional abundance for xenobiotic degradation in two samples. Among cultivated bacteria which could be promising producers of secreted RNase the representatives of Bacillus and Lysinibacillus genera were found. Our results contribute to improve our understanding of richness and ecological role of Laptev Sea microbiota.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":"69 1","pages":"24-33"},"PeriodicalIF":1.2,"publicationDate":"2023-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9666154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced production of recombinant proteins in Corynebacterium glutamicum using a molecular chaperone. 利用分子伴侣增强谷氨酸棒状杆菌重组蛋白的产生。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2023-06-22 DOI: 10.2323/jgam.2022.10.002

Yali Wang, Xiuxia Liu, Ye Li, Yankun Yang, Chunli Liu, Robert J Linhardt, Fuming Zhang, Zhonghu Bai

{"title":"Enhanced production of recombinant proteins in Corynebacterium glutamicum using a molecular chaperone.","authors":"Yali Wang, Xiuxia Liu, Ye Li, Yankun Yang, Chunli Liu, Robert J Linhardt, Fuming Zhang, Zhonghu Bai","doi":"10.2323/jgam.2022.10.002","DOIUrl":"https://doi.org/10.2323/jgam.2022.10.002","url":null,"abstract":"Protein synthesis in Corynebacterium glutamicum is critical for applications in biotechnology and medicine. However, the use of C. glutamicum for protein production is limited by its low expression and aggregation. To overcome these limitations, a molecular chaperone plasmid system was developed in this study to improve the efficiency of recombinant protein synthesis in C. glutamicum. The effect of molecular chaperones on target protein synthesis (Single-chain variable fragment, Scfv) under three different promoter strengths was tested. In addition, the plasmid containing the molecular chaperone and target protein was verified for growth stability and plasmid stability. This expression model was further validated using two recombinant proteins, human interferon-beta (Hifn) and hirudin variant III (Rhv3). Finally, the Rhv3 protein was purified, and analysis of Rhv3 activity confirmed that the use of a molecular chaperone led to an improvement in test protein synthesis. Thus, the use of molecular chaperones is believed to will improve recombinant proteins synthesis in C. glutamicum.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":"69 1","pages":"34-44"},"PeriodicalIF":1.2,"publicationDate":"2023-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10030905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Stepwise metabolic engineering of Corynebacterium glutamicum for the production of phenylalanine. 谷氨酸棒状杆菌生产苯丙氨酸的逐步代谢工程。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2023-06-22 DOI: 10.2323/jgam.2022.08.002

Naoya Kataoka, Mienosuke Matsutani, Kazunobu Matsushita, Toshiharu Yakushi

{"title":"Stepwise metabolic engineering of Corynebacterium glutamicum for the production of phenylalanine.","authors":"Naoya Kataoka, Mienosuke Matsutani, Kazunobu Matsushita, Toshiharu Yakushi","doi":"10.2323/jgam.2022.08.002","DOIUrl":"https://doi.org/10.2323/jgam.2022.08.002","url":null,"abstract":"Corynebacterium glutamicum was metabolically engineered to produce phenylalanine, a valuable aromatic amino acid that can be used as a raw material in the food and pharmaceutical industries. First, a starting phenylalanine-producer was constructed by overexpressing tryptophan-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase and phenylalanine- and tyrosine-insensitive bifunctional enzyme chorismate mutase prephenate dehydratase from Escherichia coli, followed by the inactivation of enzymes responsible for the formation of dihydroxyacetone and the consumption of shikimate pathway-related compounds. Second, redirection of the carbon flow from tyrosine to phenylalanine was attempted by deleting of the tyrA gene encoding prephenate dehydrogenase, which catalyzes the committed step for tyrosine biosynthesis from prephenate. However, suppressor mutants were generated, and two mutants were isolated and examined for phenylalanine production and genome sequencing. The suppressor mutant harboring an amino acid exchange (L180R) on RNase J, which was experimentally proven to lead to a loss of function of the enzyme, showed significantly enhanced production of phenylalanine. Finally, modifications of phosphoenolpyruvate-pyruvate metabolism were investigated, revealing that the inactivation of either phosphoenolpyruvate carboxylase or pyruvate carboxylase, which are enzymes of the anaplerotic pathway, is an effective means for improving phenylalanine production. The resultant strain, harboring a phosphoenolpyruvate carboxylase deficiency, synthesized 50.7 mM phenylalanine from 444 mM glucose. These results not only provided new insights into the practical mutations in constructing a phenylalanine-producing C. glutamicum but also demonstrated the creation of a potential strain for the biosynthesis of phenylalanine-derived compounds represented by plant secondary metabolites.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":"69 1","pages":"11-23"},"PeriodicalIF":1.2,"publicationDate":"2023-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9674716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A YAK1-type protein kinase, triacylglycerol accumulation regulator 1, in the green alga Chlamydomonas reinhardtii is a potential regulator of cell division and differentiation into gametes during photoautotrophic nitrogen deficiency. 莱茵衣藻(Chlamydomonas reinhardtii)中的一种yak1型蛋白激酶(triacylglycerol accumulation regulator 1)是光自养缺氮条件下细胞分裂和分化为配子的潜在调节因子。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2023-06-22 DOI: 10.2323/jgam.2022.08.001

Yoshinori Tsuji, Akari Kinoshita, Mizuho Tsukahara, Takumi Ishikawa, Haruka Shinkawa, Takashi Yamano, Hideya Fukuzawa

{"title":"A YAK1-type protein kinase, triacylglycerol accumulation regulator 1, in the green alga Chlamydomonas reinhardtii is a potential regulator of cell division and differentiation into gametes during photoautotrophic nitrogen deficiency.","authors":"Yoshinori Tsuji, Akari Kinoshita, Mizuho Tsukahara, Takumi Ishikawa, Haruka Shinkawa, Takashi Yamano, Hideya Fukuzawa","doi":"10.2323/jgam.2022.08.001","DOIUrl":"https://doi.org/10.2323/jgam.2022.08.001","url":null,"abstract":"Yet another kinase (YAK) 1 is a conserved eukaryotic protein kinase coordinating growth and development. We previously isolated a mutant of Chlamydomonas reinhardtii defective in the YAK1 ortholog triacylglycerol (TAG) accumulation regulator 1 (TAR1). The mutant tar1-1 displayed higher levels of chlorophyll, starch, TAG, and biomass than the parental strain C9 (renamed as C9-3) in photoautotrophic nitrogen (N)-deficient conditions. However, we found that the parental C9-3 showed faster chlorosis upon N-deficiency than the original C9 (C9-1) freshly recovered from cryopreservation, suggesting that C9-3 had acquired particular characteristics during long-term subculturing. To exclude phenotypes dependent on a particular parental strain, we newly created tar1 mutants from two wild-types, C9-1 and CC 125. Like tar1-1, the new tar1 mutants showed higher levels of chlorophyll and TAG/starch than the parental strain. Upon removal of N, Chlamydomonas cells divide once before ceasing further division. Previously, the single division after N-removal was arrested in tar1-1 in photomixotrophic conditions, but this phenotype was not observed in photoautotrophic conditions because of the particular characteristics of the parental C9-3. However, using C9- 1 and CC-125 as parental strains, we showed that cell division after N-removal was impaired in new tar1 mutants in photoautotrophic conditions. Consistent with the view that the division under N-deficiency is necessary for gametic differentiation, new tar1 mutants showed lower mating efficiency than the parental strains. Taken together, TAR1 was suggested to promote differentiation into gametes through the regulation of cell division in response to N-deficiency.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":"69 1","pages":"1-10"},"PeriodicalIF":1.2,"publicationDate":"2023-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10047977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Genetic analysis of Bacillus subtilis stable L-forms obtained via long-term cultivation. 通过长期培养获得的枯草芽孢杆菌稳定 L 型的遗传分析。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2023-06-22 Epub Date: 2022-11-15 DOI: 10.2323/jgam.2022.10.003

Kazuki Ohta, Tenma Shimizu, Taku Oshima, Norikazu Ichihashi

引用次数: 0

Correction. 修正。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2023-03-10 DOI: 10.2323/jgam.2023.03.001

引用次数: 0

Isolation and characterization of Zygosaccharomyces sp. yeast strains from miso. 味噌中Zygosaccharomyces sp.酵母菌的分离与鉴定。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2023-03-06 DOI: 10.2323/jgam.2022.07.002

Tomoko Fujiwara, Atsuko Matsura, Momoka Fukuda, Katsuaki Kuroki, Tomoo Ogata

{"title":"Isolation and characterization of Zygosaccharomyces sp. yeast strains from miso.","authors":"Tomoko Fujiwara, Atsuko Matsura, Momoka Fukuda, Katsuaki Kuroki, Tomoo Ogata","doi":"10.2323/jgam.2022.07.002","DOIUrl":"https://doi.org/10.2323/jgam.2022.07.002","url":null,"abstract":"There is currently great interest in the salt-tolerant yeast strains used to produce miso and soy sauce. Since the isolation of Zygosaccharomyces sp. strain from Japanese miso more than 60 years, several hybrid strains have been identified in fermented foods. Studies have shown that the active mating-type locus of the original Zygosaccharomyces sp. yeast strain is located between the T-subgenome sequence and the P-subgenome sequence. In this study, 32 salt-tolerant Zygosaccharomyces sp. yeast strains were isolated from five miso factories in Hiroshima Prefecture, Japan. Analysis by flow cytometry revealed that 27 strains were diploid and five strains were haploid. PCR analysis indicated that the 27 diploid strains had the same chromosomal structure of the active mating-type (MAT) locus as the original yeast strain isolated from miso 60 years ago. In addition, the 27 diploid strains were allodiploid, namely, natural hybrids of Z. rouxii and a related species, while the five haploid strains were all Z. rouxii. We found that cells of yeast strains isolated from miso changed morphologically when co-cultured with a yeast strain of opposite mating-type under nitrogen starvation conditions. The DNA sequence of the active mating-type locus and the results of cell morphology changes by co-culture were consistent with the mating type of each strain shown in the mating experiments. These findings will be useful for the future production of miso and soy sauce.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":"68 6","pages":"295-302"},"PeriodicalIF":1.2,"publicationDate":"2023-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10852723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial degradation of low-density polyethylene by Neopestalotiopsis phangngaensis. 新拟盘多毛孢对低密度聚乙烯的微生物降解。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2023-03-06 DOI: 10.2323/jgam.2022.07.001

Sarunpron Khruengsai, Teerapong Sripahco, Patcharee Pripdeevech

{"title":"Microbial degradation of low-density polyethylene by Neopestalotiopsis phangngaensis.","authors":"Sarunpron Khruengsai, Teerapong Sripahco, Patcharee Pripdeevech","doi":"10.2323/jgam.2022.07.001","DOIUrl":"https://doi.org/10.2323/jgam.2022.07.001","url":null,"abstract":"Low-density polyethylene (LDPE) has been commercially used and accumulated as plastic solid waste. LDPE has also been found to be a non-degradable waste for decades and found as a pollution source in the environment. In this study, 65 fungi were screened for their biodegradation of LDPE. The fungi Neopestalotiopsis phangngaensis, Alternaria burnsii, Alternaria pseudoeichhorniae, and Arthrinium sacchari showed significant potential in LDPE biodegradation. These fungi were individually cultured with an LDPE sheet as a carbon source for 90 days. A maximum weight loss of the LDPE sheet was detected by the fungus N. phangngaensis (54.34%). This fungus also revealed the highest reduction rate of tensile strength of the LDPE sheet (0.33 MPa). The morphological surface of LDPE culturing with N. phangngaensis was crack, pit, and rough analyzed by scanning electron microscopy. The biodegradation of the LDPE sheet by N. phangngaensis was also confirmed by the Sturm test and analysis of enzymatic activities. The Sturm test showed the highest decomposition of the LDPE sheet by N. phangngaensis into CO2 with 2.14 g/L after incubation. Enzymatic activities of laccase, manganese peroxidase, and lignin peroxidase enzymes were found by N. phangngaensis during the LDPE degradation. The volatile organic compounds in culture supernatant of N. phangngaensis were also investigated. The major compounds were 3Z-diethyl acetal hexenal, 2E,4E-decadienol, and 2Z-diethyl acetal hexenal. This study reveals the utilization of the fungus N. phangngaensis as the carbon source at a considerable biodegradation rate without any prior treatment. Therefore, the fungus N. phangngaensis may be applied as an alternative degrader for LDPE degradation in the environment.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":"68 6","pages":"287-294"},"PeriodicalIF":1.2,"publicationDate":"2023-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9399216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

16S rRNA gene sequencing reveals an altered composition of gut microbiota in children with Mycoplasma pneumoniae pneumonia treated with azithromycin. 16S rRNA基因测序揭示了阿奇霉素治疗肺炎支原体肺炎患儿肠道菌群组成的改变。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2023-03-06 DOI: 10.2323/jgam.2022.05.004

Qiong Deng, Zhu Wang, Pengmei Wu, Hui Liang, Haixia Wu, Lirong Zhang, Jing Ying

{"title":"16S rRNA gene sequencing reveals an altered composition of gut microbiota in children with Mycoplasma pneumoniae pneumonia treated with azithromycin.","authors":"Qiong Deng, Zhu Wang, Pengmei Wu, Hui Liang, Haixia Wu, Lirong Zhang, Jing Ying","doi":"10.2323/jgam.2022.05.004","DOIUrl":"https://doi.org/10.2323/jgam.2022.05.004","url":null,"abstract":"Mycoplasma pneumoniae is one of the most important pathogens causing community acquired pneumonia in children, and the pathogenic mechanism of M. pneumoniae infection is complex. Azithromycin is an effective agent for treating the acquired lower respiratory tract infection and urogenital tract infection with slight adverse reactions. This study aimed to compare the intestinal microflora before (PP1) and after azithromycin intervention (PP2) in children with pneumonia caused by M. pneumoniae, combined with body fluid biochemical analysis to determine the intestinal flora affecting the progress of the disease. Fifteen children diagnosed with M. pneumoniae pneumonia were recruited. The fecal samples and clinical biochemical data were collected. 16S rRNA gene amplicon sequencing and bioinformatics analysis were conducted by the Beijing Genomics Institute. The operational taxonomic unit abundance analysis showed significant differences between the two groups. The species richness analysis showed differences in class, family, genus, order, species, and phylum. The abundance of Haemophilus, Pasteurellales, and Pasteurellaceae was found to be significantly higher in the PP1 group. The Pearson correlation analysis showed that the microbes strongly correlated with the clinical features. 16S rRNA gene sequencing data revealed altered composition of gut microbiota in children with M. pneumoniae pneumonia treated with azithromycin. The altered expression of microbes correlated with clinical features, which might help diagnose and treat the disease.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":"68 6","pages":"253-261"},"PeriodicalIF":1.2,"publicationDate":"2023-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9398757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Purification and characterization of lignin peroxidase from white-rot fungi Pleurotus pulmonarius CPG6 and its application in decolorization of synthetic textile dyes. 白腐菌Pleurotus pulmonarius CPG6中木质素过氧化物酶的纯化、表征及其在纺织合成染料脱色中的应用。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2023-03-06 DOI: 10.2323/jgam.2022.05.005

Vu Dinh Giap, Hoang Thanh Duc, Pham Thi Mai Huong, Do Thi Hanh, Do Huu Nghi, Vu Dinh Duy, Dang Thu Quynh

{"title":"Purification and characterization of lignin peroxidase from white-rot fungi Pleurotus pulmonarius CPG6 and its application in decolorization of synthetic textile dyes.","authors":"Vu Dinh Giap, Hoang Thanh Duc, Pham Thi Mai Huong, Do Thi Hanh, Do Huu Nghi, Vu Dinh Duy, Dang Thu Quynh","doi":"10.2323/jgam.2022.05.005","DOIUrl":"https://doi.org/10.2323/jgam.2022.05.005","url":null,"abstract":"From the biotechnological point of view, enzymes are powerful tools that help sustain a clean environment in several ways. The enzymatic biodegradation of synthetic dyes is a promising goal since it reduces pollution caused by textile dyeing factory wastewater. Lignin peroxidase (EC 1.11.1.14, LiP) has high redox potential; thus, it is great for application in various industrial fields (e.g., paper- waste treatment and textile dyeing wastewater treatment). In the present study, a LiP from an isolated strain Pleurotus pulmonarius CPG6 (PpuLiP) was successfully purified with a specific activity of 6.59 U mg -1. The enzyme was purified by using three-step column chromatography procedures including DEAE, Sephadex G-75, and HiTrapTM Q FF columns with 17.8-fold purity. The enzyme with a molecular weight of 40 kDa exhibited enhanced pH stability in the acidic range. The activity retention was over 75% at a pH of 3.0 for more than 6 hours. Purified PpuLiP was able to oxidize a variety of substrates including veratryl alcohol, 2,4-DCP, n propanol, and guaiacol. The effect of metal ions on PpuLiP activity was analyzed. The study will provide a ground to decolorize dyes from various groups of PpuLiP. Purified PpuLiP could decolorize 35% Acid blue 25 (AB25), 50% Acid red 129 (AB129), 72% Acid blue 62 (NY3), 85% Acid blue 113 (AB113), 55% Remazol Brilliant blue R (RBBR), and 100% Reactive red 120 (RR120) for 12 hours. Most of the dyes were decolorized, but the heat-denatured enzyme used as negative control obviously did not decolorize the tested dyes. These results indicate that the PpuLiP has potential application in enzyme-based decolorization of synthetic dyes. Keywords: Decolorization; lignin peroxidase; Pleurotus pulmonarius; textile dyes.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":"68 6","pages":"262-269"},"PeriodicalIF":1.2,"publicationDate":"2023-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10852251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3