不同条件下生长的嗜热热菌HB8的脂质谱分析。

IF 0.8 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Masahiko Kawaguchi, Haruo Shimada, Yoshitaka Bessho, Naoki Nemoto
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引用次数: 0

摘要

热蝇属植物的膜脂具有独特的结构。迄今为止,在嗜热热菌HB8中只发现了四种极性脂质;即两种磷酸糖脂和两种糖脂,它们都有三个支链脂肪酸。其他脂质分子可能存在;然而,到目前为止,他们还没有被确认。为了明确嗜热T. HB8的整体脂质谱,我们在4种不同的生长(温度和/或营养)条件下对其进行了培养,并分别采用高效薄层色谱(HPTLC)和气相色谱-质谱(GC - MS)分析了其极性脂质和脂肪酸的组成。在HPTLC板上检测到31个脂质点,并根据磷酸盐、氨基和糖基的存在与否进行了分析。然后,我们给所有的点分配了ID号。对这些极性脂质的比较分析表明,在高温和最低培养基条件下,脂质分子的多样性增加。特别是在高温条件下,氨基酸种类增加。在GC-MS脂肪酸对比中,该生物中不常见的同支偶数碳原子在最低培养基条件下显著增加,说明不同营养条件下脂肪酸端支链氨基酸种类不同。在本研究中,检测到几种未识别的脂质,阐明脂质结构将为研究细菌的环境适应性提供重要信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Profiling of lipids in Thermus thermophilus HB8 grown under various conditions.

The membrane lipids of Thermus species have unique structures. Only four polar lipid species have so far been identified in Thermus thermophilus HB8; namely, are two phosphoglycolipids and two glycolipids, both of which have three branched fatty acid chains. Other lipid molecules may be present; however, they have not been identified so far. To clarify the whole lipid profile of T. thermophilus HB8, we cultured this organism under four different growth (temperature and/or nutrition) conditions and analyzed the compositions of polar lipids and fatty acids by high-performance thin-layer chromatography (HPTLC) and gas chromatograph-mass spectrometry (GCーMS), respectively. Thirty-one lipid spots were detected on HPTLC plates and profiled in terms of the presence or absence of phosphate, amino, and sugar groups. Then, we allocated ID numbers to all the spots. Comparative analyses of these polar lipids showed that the diversity of lipid molecules increased under high temperature and minimal medium conditions. In particular, aminolipid species increased under high temperature conditions. As for the fatty acid comparison by GC-MS, iso-branched even-numbered carbon atoms, which are unusual in this organism, significantly increased under the minimal medium condition, suggesting that kinds of branched amino acids at the fatty acid terminus varies under different nutrition conditions. In this study, several unidentified lipids were detected, and elucidation of the lipid structures will provide important information on the environmental adaptation of bacteria.

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来源期刊
Journal of General and Applied Microbiology
Journal of General and Applied Microbiology 生物-生物工程与应用微生物
CiteScore
2.40
自引率
0.00%
发文量
42
审稿时长
6-12 weeks
期刊介绍: JGAM is going to publish scientific reports containing novel and significant microbiological findings, which are mainly devoted to the following categories: Antibiotics and Secondary Metabolites; Biotechnology and Metabolic Engineering; Developmental Microbiology; Environmental Microbiology and Bioremediation; Enzymology; Eukaryotic Microbiology; Evolution and Phylogenetics; Genome Integrity and Plasticity; Microalgae and Photosynthesis; Microbiology for Food; Molecular Genetics; Physiology and Cell Surface; Synthetic and Systems Microbiology.
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