Journal of Extracellular Vesicles最新文献

{"title":"Scalable production of siRNA-encapsulated extracellular vesicles for the inhibition of KRAS-mutant cancer using acoustic shock waves","authors":"Hyo Kyeong Kim,&nbsp;Yujeong Choi,&nbsp;Kyoung Hwa Kim,&nbsp;Yeongju Byun,&nbsp;Tae Hee Kim,&nbsp;Jae Hwan Kim,&nbsp;Shung Hyun An,&nbsp;DaeHo Bae,&nbsp;Myeong Kwan Choi,&nbsp;Minyoung Lee,&nbsp;Gwansuk Kang,&nbsp;Jihwa Chung,&nbsp;Seok-Hyun Kim,&nbsp;Kihwan Kwon","doi":"10.1002/jev2.12508","DOIUrl":"https://doi.org/10.1002/jev2.12508","url":null,"abstract":"<p>Extracellular vesicles (EVs) have emerged as a potential delivery vehicle for nucleic-acid-based therapeutics, but challenges related to their large-scale production and cargo-loading efficiency have limited their therapeutic potential. To address these issues, we developed a novel “shock wave extracellular vesicles engineering technology” (SWEET) as a non-genetic, scalable manufacturing strategy that uses shock waves (SWs) to encapsulate siRNAs in EVs. Here, we describe the use of the SWEET platform to load large quantities of KRAS^G12C-targeting siRNA into small bovine-milk-derived EVs (sBMEVs), with high efficiency. The siRNA-loaded sBMEVs effectively silenced oncogenic KRAS^G12C expression in cancer cells; they inhibited tumour growth when administered intravenously in a non-small cell lung cancer xenograft mouse model. Our study demonstrates the potential for the SWEET platform to serve as a novel method that allows large-scale production of cargo-loaded EVs for use in a wide range of therapeutic applications.</p>","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":null,"pages":null},"PeriodicalIF":15.5,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jev2.12508","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142324670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Benchmarking transcriptome deconvolution methods for estimating tissue- and cell-type-specific extracellular vesicle abundances","authors":"Jannik Hjortshøj Larsen,&nbsp;Iben Skov Jensen,&nbsp;Per Svenningsen","doi":"10.1002/jev2.12511","DOIUrl":"https://doi.org/10.1002/jev2.12511","url":null,"abstract":"<p>Extracellular vesicles (EVs) contain cell-derived lipids, proteins and RNAs; however, determining the tissue- and cell-type-specific EV abundances in body fluids remains a significant hurdle for our understanding of EV biology. While tissue- and cell-type-specific EV abundances can be estimated by matching the EV's transcriptome to a tissue's/cell type's expression signature using deconvolutional methods, a comparative assessment of deconvolution methods' performance on EV transcriptome data is currently lacking. We benchmarked 11 deconvolution methods using data from four cell lines and their EVs, in silico mixtures, 118 human plasma and 88 urine EVs. We identified deconvolution methods that estimated cell type-specific abundances of pure and in silico mixed cell line-derived EV samples with high accuracy. Using data from two urine EV cohorts with different EV isolation procedures, four deconvolution methods produced highly similar results. The three methods were also concordant in their tissue- and cell-type-specific plasma EV abundance estimates. We identified driving factors for deconvolution accuracy and highlighted the importance of implementing biological knowledge in creating the tissue/cell type signature. Overall, our analyses demonstrate that the deconvolution algorithms DWLS and CIBERSORTx produce highly similar and accurate estimates of tissue- and cell-type-specific EV abundances in biological fluids.</p>","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":null,"pages":null},"PeriodicalIF":15.5,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jev2.12511","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142320706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular vesicles derived from melanoma cells induce carcinoma-associated fibroblasts via miR-92b-3p mediated downregulation of PTEN","authors":"Stefanie Kewitz-Hempel,&nbsp;Nicola Windisch,&nbsp;Gerd Hause,&nbsp;Lutz Müller,&nbsp;Cord Sunderkötter,&nbsp;Dennis Gerloff","doi":"10.1002/jev2.12509","DOIUrl":"10.1002/jev2.12509","url":null,"abstract":"<p>In melanoma, carcinoma-associated fibroblasts (CAFs) are important cellular components in the tumour microenvironment due to their potential to promote tumour growth and metastatic spread of malignant cells. Melanoma cells have the ability to affect non-tumour cells in the microenvironment by releasing extracellular vesicles (EVs). The mechanisms responsible for reprogramming normal dermal fibroblasts (NHDFs) into CAFs remain incompletely understood. However, it is likely thought to be mediated by melanoma-specific miRNAs, which are transported by EVs derived from melanoma cells. Therefore, we wondered if one of the most enriched miRNAs in EVs secreted by melanoma cells, miR-92b-3p, is involved in the conversion of normal fibroblasts into CAFs. We observed that melanoma cell-derived EVs indeed delivered miR-92b-3p into NHDFs and that its accumulation correlated with CAF formation, as demonstrated by enhanced expression of CAF marker genes and increased proliferation and migration. Overexpression of miR-92b-3p in NHDFs revealed similar results, while EVs deficient of miR-92b-3p did not induce a CAF phenotype. As a target we identified PTEN, whose repression led to increased expression of CAF markers. We thus provide a novel pathway of intercellular communication by which melanoma cells control the transformation of CAFs by virtue of EV-transported miRNAs.</p>","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":null,"pages":null},"PeriodicalIF":15.5,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jev2.12509","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142307876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing protective immunity against bacterial infection via coating nano-Rehmannia glutinosa polysaccharide with outer membrane vesicles","authors":"Yee Huang,&nbsp;Jiaying Sun,&nbsp;Xuemei Cui,&nbsp;Xuefeng Li,&nbsp;Zizhe Hu,&nbsp;Quanan Ji,&nbsp;Guolian Bao,&nbsp;Yan Liu","doi":"10.1002/jev2.12514","DOIUrl":"10.1002/jev2.12514","url":null,"abstract":"<p>With the coming of the post-antibiotic era, there is an increasingly urgent need for safe and efficient antibacterial vaccines. Bacterial outer membrane vesicles (OMVs) have received increased attention recently as a potential subunit vaccine. OMVs are non-replicative and contain the principle immunogenic bacterial antigen, which circumvents the safety concerns of live-attenuated vaccines. Here, we developed a novel nano-vaccine by coating OMVs onto PEGylated nano-<i>Rehmannia glutinosa</i> polysaccharide (pRL) in a structure consisting of concentric circles, resulting in a more stable vaccine with improved immunogenicity. The immunological function of the pRL-OMV formulation was evaluated in vivo and in vitro, and the underlying mechanism was studied though transcriptomic analysis. The pRL-OMV formulation significantly increased dendritic cell (DC) proliferation and cytokine secretion. Efficient phagocytosis of the formulation by DCs was accompanied by DC maturation. Further, the formulation demonstrated superior lymph node targeting, contributing to a potent mixed cellular response and bacterial-specific antibody response against <i>Bordetella bronchiseptica</i> infection. Specifically, transcriptomic analysis revealed that the immune protection function correlated with T-cell receptor signalling and Th1/Th2/Th17 differentiation, among other markers of enhanced immunological activity. These findings have implications for the future application of OMV-coated nano-carriers in antimicrobial immunotherapy.</p>","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":null,"pages":null},"PeriodicalIF":15.5,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jev2.12514","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142307875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma-derived extracellular vesicles (EVs) as biomarkers of sepsis in burn patients via label-free Raman spectroscopy","authors":"Hannah J. O'Toole,&nbsp;Neona M. Lowe,&nbsp;Vishalakshi Arun,&nbsp;Anna V. Kolesov,&nbsp;Tina L. Palmieri,&nbsp;Nam K. Tran,&nbsp;Randy P. Carney","doi":"10.1002/jev2.12506","DOIUrl":"https://doi.org/10.1002/jev2.12506","url":null,"abstract":"<p>Sepsis following burn trauma is a global complication with high mortality, with ∼60% of burn patient deaths resulting from infectious complications. Diagnosing sepsis is complicated by confounding clinical manifestations of the burn injury, and current biomarkers lack the sensitivity and specificity required for prompt treatment. There is a strong rationale to assess circulating extracellular vesicles (EVs) from patient liquid biopsy as sepsis biomarkers due to their release by pathogens from bacterial biofilms and roles in the subsequent immune response. This study applies Raman spectroscopy to patient plasma-derived EVs for rapid, sensitive, and specific detection of sepsis in burn patients, achieving 97.5% sensitivity and 90.0% specificity. Furthermore, spectral differences between septic and non-septic burn patient EVs could be traced to specific glycoconjugates of bacterial strains associated with sepsis morbidity. This work illustrates the potential application of EVs as biomarkers in clinical burn trauma care and establishes Raman analysis as a fast, label-free method to specifically identify features of bacterial EVs relevant to infection amongst the host background.</p>","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":null,"pages":null},"PeriodicalIF":15.5,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jev2.12506","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142273133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of mesenchymal stem cell-derived extracellular vesicles in SARS-CoV-2 and H1N1 influenza-induced acute lung injury","authors":"Jun Ho Lee,&nbsp;Hyungtaek Jeon,&nbsp;Jan Lötvall,&nbsp;Byong Seung Cho","doi":"10.1002/jev2.12495","DOIUrl":"https://doi.org/10.1002/jev2.12495","url":null,"abstract":"<p>Mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) have shown anti-inflammatory potential in multiple inflammatory diseases. In the March 2022 issue of the <i>Journal of Extracellular Vesicles</i>, it was shown that EVs from human MSCs can suppress severe acute respiratory distress syndrome, coronavirus 2 (SARS-CoV-2) replication and can mitigate the production and release of infectious virions. We therefore hypothesized that MSC-EVs have an anti-viral effect in SARS-CoV-2 infection in vivo. We extended this question to ask whether also other respiratory viral infections could be treated by MSC-EVs. Adipose stem cell-derived EVs (ASC-EVs) were isolated using tangential flow filtration from conditioned media obtained from a multi-flask cell culture system. The effects of the ASC-EVs were tested  in Vero E6 cells in vitro. ASC-EVs were also given i.v. to SARS-CoV-2 infected Syrian Hamsters, and H1N1 influenza virus infected mice. The ASC-EVs attenuated SARS-CoV-2 virus replication in Vero E6 cells and reduced body weight and signs of lung injury in infected Syrian hamsters. Furthermore, ASC-EVs increased the survival rate of influenza A-infected mice and attenuated signs of lung injury. In summary, this study suggests that ASC-EVs can have beneficial therapeutic effects in models of virus-infection-associated acute lung injury and may potentially be developed to treat lung injury in humans.</p>","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":null,"pages":null},"PeriodicalIF":15.5,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jev2.12495","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142165630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular vesicle analytical science loses a touch of creativity and kindness","authors":"Paolo Bergese,&nbsp;Marcella Chiari,&nbsp;Alessandro Gori,&nbsp;Benedetta Bussolati,&nbsp;Pietro Parisse,&nbsp;the EVIta Board","doi":"10.1002/jev2.12504","DOIUrl":"https://doi.org/10.1002/jev2.12504","url":null,"abstract":"&lt;p&gt;&lt;/p&gt;&lt;p&gt;It is with profound sadness and sorrow that we announce the unexpected passing of our cherished friend and colleague, Marina Cretich. Marina, a renowned scientist in the field of bioanalytical chemistry, passed away on June 29th 2024, leaving behind a heritage of invaluable contributions to science. Her career was marked by a relentless pursuit of knowledge and an unwavering dedication to advancing scientific understanding.&lt;/p&gt;&lt;p&gt;Marina graduated in Biological Sciences with a specialization in Molecular Biology from the University of Milano in 1998 with Prof. Piergiorgio Righetti. She then joined the National Research Council of Italy (CNR), where she served as a Researcher at the Institute of Chemistry for Molecular Recognition in Milan, becoming a key figure in the Analytical Microsystem laboratory guided by Dr. Marcella Chiari. Thanks to the interdisciplinarity of her approach, she made significant strides in the progress of advanced methods and materials for bio-molecular recognition, together with high-sensitive and selective biosensors, providing new tools for detecting biomolecules with unprecedented accuracy and efficiency. Her strong focus on integrating bioanalytical techniques, microfluidics and detection tools, enabled more rapid and precise analyses, enhancing the capabilities of lab-on-a-chip devices, towards point-of-care diagnostics.&lt;/p&gt;&lt;p&gt;These studies paved the road to her approach to extracellular vesicles, addressing the challenges posed by their separation and analysis. Specifically, Marina was working on affinity-capturing protocols from complex samples for EV isolation and on microarray platforms for their molecular characterization (Daaboul et al., &lt;span&gt;2016&lt;/span&gt;). Her interest in the field was raised by participating in the EU project INDEX, coordinated by Dr Marcella Chiari, and became true love for this fascinating and challenging area. This led Marina to establish her own research team and to find the Extracellular Vesicle Lab at SCITEC-CNR. Soon after, she was the Coordinator of the EU project MARVEL, a multi-partner project at the intersection of chemistry and technology, biology and translational medicine, which was centred around the use of membrane-sensing peptides (MSP) as enabling tools for the multiscale EV isolation (Gori et al., &lt;span&gt;2020&lt;/span&gt;, Gori et al, &lt;span&gt;2024&lt;/span&gt;). She was a true pioneer in this field and she consolidated a leading expertise in the area of ultrasensitive EV analysis, aiming to fill existing gaps in the clinical translation of EVs in diagnostics. Her contribution is witnessed by remarkable scientific contributions (Frigerio et al., &lt;span&gt;2022&lt;/span&gt;; Musicò et al., &lt;span&gt;2024&lt;/span&gt;) and, despite very recent, the concepts and technologies that she developed set the basis for an ever-increasing number of preclinical and clinical collaborations encompassing EV analysis in the fields of neurodegeneration, cancer, and heart diseases.&lt;/p&gt;&lt;p&gt;In recent years, she also dedic","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":null,"pages":null},"PeriodicalIF":15.5,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jev2.12504","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142165745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The activity of the quorum sensing regulator HapR is modulated by the bacterial extracellular vesicle (BEV)-associated protein ObfA of Vibrio cholerae","authors":"Stephan P. Ebenberger,&nbsp;Fatih Cakar,&nbsp;Yi-Chi Chen,&nbsp;Katharina Pressler,&nbsp;Leo Eberl,&nbsp;Stefan Schild","doi":"10.1002/jev2.12507","DOIUrl":"https://doi.org/10.1002/jev2.12507","url":null,"abstract":"<p><i>Vibrio cholerae</i>, a facultative human pathogen and causative agent of the severe diarrheal disease cholera, transits between the human intestinal tract and aquatic reservoirs. Like other bacterial species, <i>V. cholerae</i> continuously releases bacterial extracellular vesicles (BEVs) from its surface, which have been recently characterised for their role during in vivo colonisation. However, between epidemic outbreaks, <i>V. cholerae</i> persists in the biofilm mode for extended periods in aquatic reservoirs, which enhances environmental fitness and host transition. In this study, we investigated the effect of <i>V. cholerae</i> BEVs on biofilm formation, a critical feature for ex vivo survival. In contrast to BEVs from planktonic cultures, our results show that physiological concentrations of BEVs from dynamic biofilm cultures facilitate <i>V. cholerae</i> biofilm formation, which could be linked to a proteinaceous factor. Comparative proteomic analyses of planktonic- and biofilm-derived BEVs identified a previously uncharacterised outer membrane protein as an abundant component of dynamic biofilm-derived BEVs, which was found to be responsible for the BEV-dependent enhancement of biofilm production. Consequently, this protein was named <b>o</b>uter membrane-associated <b>b</b>iofilm <b>f</b>acilitating protein <b>A</b> (ObfA). Comprehensive molecular studies unravelled ObfA as a negative modulator of HapR activity. HapR is a key transcriptional regulator of the <i>V. cholerae</i> quorum sensing (QS) cascade acting as a potent repressor of biofilm formation and virulence. Consistently, <i>obfA</i> mutants not only exhibited reduced biofilm production but also reduced colonisation fitness. Surprisingly, our results demonstrate that ObfA does not affect HapR through the canonical QS system but via the Csr-cascade altering the expression of the small regulatory RNAs CsrC and CsrD. In summary, this study elucidates a novel intraspecies BEV-based communication in <i>V. cholerae</i> that influences biofilm formation and colonisation fitness via a new regulatory pathway involving HapR, Csr-cascade and the BEV-associated protein ObfA.</p>","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":null,"pages":null},"PeriodicalIF":15.5,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jev2.12507","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142165748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The 8-oxoguanine DNA glycosylase-synaptotagmin 7 pathway increases extracellular vesicle release and promotes tumour metastasis during oxidative stress","authors":"Ying Ma,&nbsp;Jiarong Guo,&nbsp;Haipeng Rao,&nbsp;Jingyu Xin,&nbsp;Xinyi Song,&nbsp;Rui Liu,&nbsp;Shan Shao,&nbsp;Jiajia Hou,&nbsp;Liyu Kong,&nbsp;Zhigang Hu,&nbsp;Lingfeng He,&nbsp;Feiyan Pan,&nbsp;Zhigang Guo","doi":"10.1002/jev2.12505","DOIUrl":"10.1002/jev2.12505","url":null,"abstract":"<p>Reactive oxygen species (ROS)-induced oxidative DNA damages have been considered the main cause of mutations in genes, which are highly related to carcinogenesis and tumour progression. Extracellular vesicles play an important role in cancer metastasis. However, the precise role of DNA oxidative damage in extracellular vesicles (EVs)-mediated cancer cell migration and invasion remains unclear. Here, we reveal that ROS-mediated DNA oxidative damage signalling promotes tumour metastasis through increasing EVs release. Mechanistically, 8-oxoguanine DNA glycosylase (OGG1) recognises and binds to its substrate 8-oxo-7,8-dihydroguanine (8-oxoG), recruiting NF-κB to the synaptotagmin 7 (SYT7) promoter and thereby triggering SYT7 transcription. The upregulation of SYT7 expression leads to increased release of E-cadherin-loaded EVs, which depletes intracellular E-cadherin, thereby inducing epithelial-mesenchymal transition (EMT). Notably, Th5487, the inhibitor of DNA binding activity of OGG1, blocks the recognition and transmission of oxidative signals, alleviates SYT7 expression and suppresses EVs release, thereby preventing tumour progression in vitro and in vivo. Collectively, our study illuminates the significance of 8-oxoG/OGG1/SYT7 axis-driven EVs release in oxidative stress-induced tumour metastasis. These findings provide a deeper understanding of the molecular basis of cancer progression and offer potential avenues for therapeutic intervention.</p>","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":null,"pages":null},"PeriodicalIF":15.5,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11375530/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142132933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An aptamer-guided fluorescence polarisation platform for extracellular vesicle liquid biopsy","authors":"Cuong Viet Pham,&nbsp;Rocky Chowdhury,&nbsp;Shweta Patel,&nbsp;Satendra Kumar Jaysawal,&nbsp;Yingchu Hou,&nbsp;Huo Xu,&nbsp;Lee Jia,&nbsp;Yu-mei Zhang,&nbsp;Xiaowei Wang,&nbsp;Wei Duan,&nbsp;Dongxi Xiang","doi":"10.1002/jev2.12502","DOIUrl":"10.1002/jev2.12502","url":null,"abstract":"<p>The translation of discoveries on extracellular vesicle (EV) based cancer biomarkers to personalised precision oncology requires the development of robust, sensitive and specific assays that are amenable to adoption in the clinical laboratory. Whilst a variety of elegant approaches for EV liquid biopsy have been developed, most of them remain as research prototypes due to the requirement of a high level of microfabrication and/or sophisticated instruments. Hence, this study is set to develop a simple DNA aptamer-enabled and fluorescence polarisation-based homogenous assay that eliminates the need to separate unbound detection ligands from the bound species for EV detection. High specificity is achieved by immobilising EVs with one set of antibodies and subsequently detecting them with a DNA aptamer targeting a distinct EV biomarker. This two-pronged strategy ensures the removal of most, if not all, non-EV substances in the input biofluids, including soluble proteins, protein aggregates or non-vesicular particles, prior to quantifying biomarker-positive EVs. A limit of detection of 5.0 × 10⁶ EVs/mL was achieved with a linear quantification range of 5.0 × 10⁸ to 2.0 × 10¹⁰ EVs/mL. Facilitated by a multiple parametric analysis strategy, this aptamer-guided fluorescence polarisation assay was capable of distinguishing EVs from three different types of solid cancer cells based on quantitative differences in the levels of the same sets of biomarkers on EVs. Given the simplicity of the method and its ease of implementation in automated clinical biochemistry analysers, this assay could be exploited for future EV-based continuous and real-time monitoring of the emergence of new macro- or micro-metastasis, cancer progression as well as the response to treatment throughout different stages of cancer management in the clinic.</p>","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":null,"pages":null},"PeriodicalIF":15.5,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jev2.12502","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142107993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}