Journal of Extracellular Vesicles最新文献

Development of CAR Exosomes Targeting FAP for the Treatment of Intrauterine Adhesion. 靶向FAP的CAR外泌体治疗宫内粘连的研究进展。

IF 14.5 1区医学

Journal of Extracellular Vesicles Pub Date : 2026-05-01 DOI: 10.1002/jev2.70284

Wenyan Fu, Kewen Qian, Hongru Ai, Yitan Zou, Yaping Zhou, Ruixue Mao, Jian Zhao, Changhai Lei, Shi Hu

{"title":"Development of CAR Exosomes Targeting FAP for the Treatment of Intrauterine Adhesion.","authors":"Wenyan Fu, Kewen Qian, Hongru Ai, Yitan Zou, Yaping Zhou, Ruixue Mao, Jian Zhao, Changhai Lei, Shi Hu","doi":"10.1002/jev2.70284","DOIUrl":"https://doi.org/10.1002/jev2.70284","url":null,"abstract":"Engineered extracellular vesicles (EVs) have emerged as promising cell-free platforms for immunomodulation and tissue repair. In this study, we generated EVs derived from chimeric antigen receptor (CAR) T cells targeting fibroblast activation protein (FAP) and investigated their biological and therapeutic functions. These FAP-CAR EVs effectively inhibited intrauterine fibrosis, promoted endometrial regeneration, and increased pregnancy rates in a mouse model of intrauterine adhesion. Importantly, the exosome-based therapy did not affect embryonic development or trigger systemic inflammation, indicating high safety compared with T-cell-based treatment. Mechanistically, while FAP-targeted T cells could suppress fibrosis, they also induced severe cytokine-release toxicity, which was completely avoided in the EV-based strategy. Together, these findings demonstrate that FAP is a critical target for fibrotic disease intervention and that CAR-T-derived EVs represent a safe and effective vesicle-based therapeutic modality.","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":"15 5","pages":"e70284"},"PeriodicalIF":14.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13132341/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147816179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GCC2 in Small Extracellular Vesicles as a Diagnostic and Prognostic Biomarker of Early-Stage Lung Adenocarcinoma. 细胞外小泡GCC2作为早期肺腺癌诊断和预后的生物标志物

IF 14.5 1区医学

Journal of Extracellular Vesicles Pub Date : 2026-05-01 DOI: 10.1002/jev2.70264

Byeong Hyeon Choi, Hyonggin An, Sukki Cho, Sungsoo Lee, Hyeong Ryul Kim, Jong Ho Cho, Jun Hee Lee, Ga Yoon Kim, Ok Hwa Jeon, Hyunku Shin, Yeonho Choi, Hyun Koo Kim

{"title":"GCC2 in Small Extracellular Vesicles as a Diagnostic and Prognostic Biomarker of Early-Stage Lung Adenocarcinoma.","authors":"Byeong Hyeon Choi, Hyonggin An, Sukki Cho, Sungsoo Lee, Hyeong Ryul Kim, Jong Ho Cho, Jun Hee Lee, Ga Yoon Kim, Ok Hwa Jeon, Hyunku Shin, Yeonho Choi, Hyun Koo Kim","doi":"10.1002/jev2.70264","DOIUrl":"10.1002/jev2.70264","url":null,"abstract":"Emerging evidence suggests that GRIP and coiled-coil domain-containing two enriched small extracellular vesicles (sEV-GCC2) may serve as diagnostic biomarkers of early-stage lung adenocarcinomas. However, the roles of these molecules remain unclear. This study evaluated the diagnostic and prognostic potential of sEV-GCC2 for detecting early-stage lung adenocarcinoma and its tumourigenic role in vitro and in vivo. This retrospective multicentre study analyzed 470 plasma samples (320 lung adenocarcinoma patients, 150 controls; mean follow-up: 34.7 ± 24.0 months) across five institutions. Size-exclusion chromatography and enzyme-linked immunosorbent assay were used to measure sEV-GCC2 levels, whereas immunohistochemistry was used to confirm GCC2 expression in tumour tissues. Functional studies were performed using the PC9 and H1650 lung adenocarcinoma cell lines in vitro and the corresponding PC9-based preclinical models in vivo to evaluate the tumour-related effects of sEV-GCC2. Patients exhibited significantly elevated sEV-GCC2 levels compared to controls (area under the receiver operating characteristic (ROC) curve [AUC]: 0.904, P < 0.001), with similar results in stage TisN0-T1miN0 disease (AUC: 0.781, P < 0.001). sEV-GCC2 levels were associated with the pathological TNM stage and tumour location. Higher sEV-GCC2 levels were correlated with poorer recurrence-free survival (RFS), overall survival and recurrence rates, even in patients with stage 0-IA1 disease. Functional studies have revealed that sEV-GCC2, but not GCC2-deficient sEVs, promote cancer cell proliferation, tumour growth and lymph node metastasis in vitro and in vivo. These findings highlight the diagnostic and prognostic potential of sEV-GCC2 and its tumourigenic role in early-stage lung adenocarcinoma. This study was registered at ClinicalTrials.gov (NCT04529915).","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":"15 5","pages":"e70264"},"PeriodicalIF":14.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13145344/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Outer Membrane Vesicles Mediate the Secretion and Nuclear Trafficking of a Bacterial Nucleomodulin. 外膜小泡介导细菌核调节素的分泌和核转运。

IF 14.5 1区医学

Journal of Extracellular Vesicles Pub Date : 2026-05-01 DOI: 10.1002/jev2.70286

Jack K Emery, Variya Nemidkanam, Nina Colon, Kate R J Friesen, Lena H M Le, Angus R Cramond, Maxine Yap, Mônica S Barbosa, Georgie Wray-McCann, David J McGee, Natalia Castaño-Rodríguez, Dongmei Tong, Caroline Skene, Laurent Terradot, Richard L Ferrero

{"title":"Outer Membrane Vesicles Mediate the Secretion and Nuclear Trafficking of a Bacterial Nucleomodulin.","authors":"Jack K Emery, Variya Nemidkanam, Nina Colon, Kate R J Friesen, Lena H M Le, Angus R Cramond, Maxine Yap, Mônica S Barbosa, Georgie Wray-McCann, David J McGee, Natalia Castaño-Rodríguez, Dongmei Tong, Caroline Skene, Laurent Terradot, Richard L Ferrero","doi":"10.1002/jev2.70286","DOIUrl":"https://doi.org/10.1002/jev2.70286","url":null,"abstract":"Nucleomodulins are a family of bacterial virulence proteins that traffic to the nucleus of host cells to disrupt nuclear processes and, in some cases, promote carcinogenesis. The mechanisms by which nucleomodulins are secreted and injected into host cells are not entirely clear. We hypothesised that bacterial extracellular vesicles (EVs), also known as outer membrane vesicles, may represent a novel mechanism for nucleomodulin delivery to host cells. To address this, we studied the role of EVs in the secretion of Helicobacter pylori tumour necrosis factor-α-inducing protein (Tipα), a protein that was reported to undergo nuclear trafficking and to promote carcinogenesis. Importantly, we showed that most Tipα present in H. pylori culture supernatants is associated with EVs and, moreover, is encapsulated within these particles in its biologically active dimeric form. Confocal microscopy and cell fractionation studies demonstrated that EVs carry Tipα to the nuclear compartment of host cells and can bind host DNA. EVs from isogenic tipA mutants induced greater pro-inflammatory cytokine responses in host cells than EVs from wild-type bacteria; however, this effect was dependent on the EV isolation method. We propose that EVs represent a new mechanism by which bacteria secrete and deliver nucleomodulins to their target cells, resulting in altered immune responses and possibly carcinogenesis.","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":"15 5","pages":"e70286"},"PeriodicalIF":14.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13132344/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147816155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Small Molecule H89 Facilitates Mesenchymal Stem Cell-derived Extracellular Vesicle Release and Optimizes Therapeutic Efficacy in Liver Regeneration. 小分子H89促进间充质干细胞来源的细胞外囊泡释放并优化肝脏再生的治疗效果。

IF 14.5 1区医学

Journal of Extracellular Vesicles Pub Date : 2026-05-01 DOI: 10.1002/jev2.70285

Yu Fu, Yi Ma, Jiajun Zhang, Liwei Liang, Ting Li, Zeyi Guo, Zhongzhe Li, Lei Feng, Yi Wang, Guolin He, Shao Li, Yang Li, Xiaoping Xu, Hui Liao, Yi Gao

{"title":"The Small Molecule H89 Facilitates Mesenchymal Stem Cell-derived Extracellular Vesicle Release and Optimizes Therapeutic Efficacy in Liver Regeneration.","authors":"Yu Fu, Yi Ma, Jiajun Zhang, Liwei Liang, Ting Li, Zeyi Guo, Zhongzhe Li, Lei Feng, Yi Wang, Guolin He, Shao Li, Yang Li, Xiaoping Xu, Hui Liao, Yi Gao","doi":"10.1002/jev2.70285","DOIUrl":"https://doi.org/10.1002/jev2.70285","url":null,"abstract":"The role of human umbilical cord mesenchymal stem cell-derived extracellular vesicles (hUCMSC-EVs) in liver regeneration is promising, yet their clinical translation is hampered by insufficient production. Current strategies targeting their secretion are inefficient and lack a clear mechanistic understanding. We isolated and characterized hUCMSC-EVs pretreated with the H89 and other mTORC1 inhibitors. Our findings revealed that H89 effectively enhances the secretion of hUCMSC-EVs across diverse cell types, demonstrating universal efficacy. Importantly, H89 upregulates GABARAPL1 expression, a key negative regulator of the PKA/mTORC1 pathway, to inhibit mTORC1 activity and promote the formation of amphisomes and SNARE-mediated hUCMSC-EVs release. Furthermore, EVs derived from H89-pretreated hUCMSCs (H-EVs) exhibited altered cargo composition, significantly increased proliferative activity, and potentiated liver regeneration via the RELA/miR-29a axis, which regulates the homeostasis of hepatic stellate cells. Our results highlight that H89 enhances hUCMSC-EV secretion through mTORC1 inhibition, with the resulting benefits for liver regeneration mediated by the RELA/miR-29a network. These findings demonstrate the great promise of H89 in EV-based liver regeneration, offering a promising platform for clinical translation.","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":"15 5","pages":"e70285"},"PeriodicalIF":14.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147864087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Situ Engineered "Cascade-Amplified" Drug-Loaded Vesicles for Enhanced Cancer Stem Cell Therapy. 原位工程“级联扩增”载药囊泡用于增强癌症干细胞治疗。

IF 14.5 1区医学

Journal of Extracellular Vesicles Pub Date : 2026-05-01 DOI: 10.1002/jev2.70292

Tiantian Zhang, YuanYuan Wei, Zimai Liu, Zixian Wu, Xiaoxi Wang, Kai Li, Hui Liu, Jiao Lu, Qianxi Lu, Meiyi Liu, Yongchao Wang, Zhenzhen Chen

{"title":"In Situ Engineered \"Cascade-Amplified\" Drug-Loaded Vesicles for Enhanced Cancer Stem Cell Therapy.","authors":"Tiantian Zhang, YuanYuan Wei, Zimai Liu, Zixian Wu, Xiaoxi Wang, Kai Li, Hui Liu, Jiao Lu, Qianxi Lu, Meiyi Liu, Yongchao Wang, Zhenzhen Chen","doi":"10.1002/jev2.70292","DOIUrl":"https://doi.org/10.1002/jev2.70292","url":null,"abstract":"Cancer stem cells (CSCs) characterized by the capacity of self-renewal and drug resistance, are a major cause of tumour recurrence and metastasis. However, CSCs are mainly localized in the deep and hypoxic regions of the tumour microenvironment that hinder drug penetration. Furthermore, their overexpression of the CD24/Siglec10 immune checkpoint axis markedly suppresses immune clearance, severely limiting the efficacy of current therapeutic strategies. To address this challenge, this study developed an in situ engineered \"cascade-amplified\" drug-loaded vesicle delivery system, aiming to achieve deep drug delivery into CSC-enriched regions and enhance anti-tumour immune responses. Based on a biomimetic \"core-shell\" nanoplatform (siXkr8/Dox@PMLC), this system initiates a cascade within the TME where Doxorubicin (Dox) induces tumour cells to generate drug-loaded apoptotic bodies (ApoBDs). These ApoBDs serve as primary vesicles that, upon uptake by adjacent tumour cells, trigger secondary apoptosis, establishing a \"cascade-amplified\" cycle of enhanced drug delivery. Meanwhile, the silencing of the phospholipid scramblase Xkr8 via siRNA inhibits phosphatidylserine (PS) exposure on the surface of ApoBDs, thereby preventing their recognition and clearance by M2-type macrophages and facilitating immune phenotype remodelling. Furthermore, through targeted blockade of the CD24/Siglec-10 immune axis, the nanoplatform enhances macrophage-mediated phagocytosis of CSCs. In summary, this strategy achieves deep eradication of CSCs and synergistically enhances anti-tumour immunotherapy, demonstrating significant translational potential.","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":"15 5","pages":"e70292"},"PeriodicalIF":14.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147863966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Eubacterium Rectale Derived Extracellular Vesicles Alleviate Cancer Cachexia Induced Lipolysis by Inhibiting Macrophage Polarization. 直肠真杆菌衍生的细胞外囊泡通过抑制巨噬细胞极化减轻癌症恶病质诱导的脂肪分解。

IF 14.5 1区医学

Journal of Extracellular Vesicles Pub Date : 2026-05-01 DOI: 10.1002/jev2.70293

Jiaqi Wang, Gulisudumu Maitiabula, Sirui Liu, Ruowen Li, Shijie Wang, Pinwen Zhou, Yufei Xia, Longchang Huang, Xin Chen, Xuejin Gao, Li Zhang, Xinying Wang

{"title":"The Eubacterium Rectale Derived Extracellular Vesicles Alleviate Cancer Cachexia Induced Lipolysis by Inhibiting Macrophage Polarization.","authors":"Jiaqi Wang, Gulisudumu Maitiabula, Sirui Liu, Ruowen Li, Shijie Wang, Pinwen Zhou, Yufei Xia, Longchang Huang, Xin Chen, Xuejin Gao, Li Zhang, Xinying Wang","doi":"10.1002/jev2.70293","DOIUrl":"10.1002/jev2.70293","url":null,"abstract":"Cancer cachexia (CC) is a complex pathological condition associated with cancer progression and poor prognosis, particularly in gastrointestinal cancers, and is closely linked to the gut microbiota. Lipolysis in CC may play a key role in driving cachexia progression. However, the role of the gut microbiota in CC-induced lipolysis and the underlying mechanisms remains unclear. In this study, we found that significant gut microbiota dysbiosis in pancreatic cancer cachexia, characterized by diminished abundance of Eubacterium rectale (E.rectale), was negatively associated with lipolysis severity. Furthermore, we demonstrated for the first time that E. rectale derived extracellular vesicles (EVs) can ameliorate lipolysis across multiple CC models. Mechanistically, these effects are mediated by modulating pro-inflammatory macrophages through inhibition of NF-kB signalling, reducing macrophage polarization and inflammatory cytokine secretion. Our findings indicated E. rectale EVs could offer potential therapeutic benefits for CC and emphasize the importance of host-microbiota interactions. Trial Registration: This study was registered at ClinicalTrials.gov (NCT06378853).","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":"15 5","pages":"e70293"},"PeriodicalIF":14.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13145334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RVG-Modified BMSCs-Derived Small Extracellular Vesicles Loaded With miR-21 Alleviate Neuronal Injury Resulted From Excessive Autophagy via Targeting PTEN/Akt/mTOR Pathway After Cerebral Ischaemia. rvg修饰的bmscs来源的细胞外小泡装载miR-21通过靶向PTEN/Akt/mTOR通路减轻脑缺血后过度自噬导致的神经元损伤。

IF 14.5 1区医学

Journal of Extracellular Vesicles Pub Date : 2026-05-01 DOI: 10.1002/jev2.70295

Yiyang Li, Jiacheng Hu, Jinfen Chen, Manfei Zhou, Mingchun Liao, Yayue Yang, Jiahao Zhou, Yan Han, Bin Wang, Yonghua Zhao

{"title":"RVG-Modified BMSCs-Derived Small Extracellular Vesicles Loaded With miR-21 Alleviate Neuronal Injury Resulted From Excessive Autophagy via Targeting PTEN/Akt/mTOR Pathway After Cerebral Ischaemia.","authors":"Yiyang Li, Jiacheng Hu, Jinfen Chen, Manfei Zhou, Mingchun Liao, Yayue Yang, Jiahao Zhou, Yan Han, Bin Wang, Yonghua Zhao","doi":"10.1002/jev2.70295","DOIUrl":"10.1002/jev2.70295","url":null,"abstract":"Ischaemic stroke (IS) leads to tragic disability and high adult mortality, while there are limited therapeutic measures for it. Small extracellular vesicles (sEVs) derived from bone marrow mesenchymal stem cells (BMSCs) have been suggested to have satisfactory therapeutic effects on IS by the delivery of their packed microRNA (miRNA). However, systematically administered naïve sEVs are difficult to cross the blood-brain barrier (BBB) and enter the brain parenchyma; also, the low abundance of sEVs cargo miRNAs restricts the possibility of maximising their regulatory functions. Hence, the brain targeting modification and miRNA delivery strategy are crucial for the optimisation of BMSC-sEVs therapeutic effects. In this study, based on sEVs miRNA-seq and analysis of the RNA-seq datasets for ischaemic stroke patients' samples in the GEO database, we identified that miR-21 sharply drops in the ischaemic brain. By the bioengineered BMSCs with RVG-Lamp2b peptide and miR-21-5p overexpression plasmid, RVG-miR21-sEVs were successfully developed. After characterisation of RVG-miR21-sEVs, it showed that RVG-modified sEVs had a high affinity to neurons, and the administration of RVG-miR21-sEVs displayed superior neurological functional rehabilitation and cerebral infarction reduction in the mouse transient middle cerebral artery occlusion (tMCAo) model. Additionally, RVG-miR21-sEVs treatment suppressed neuron autophagy, mitochondria dysfunction and apoptosis after oxygen-glucose deprivation/re-oxygenation (OGD/R) insult. Furthermore, by using dual-luciferase reporter assay, FISH technique, and miR-21 inhibitor and mimics transfection, we validated that the target of miR-21 is PTEN, and the mechanism investigation showed that miR-21 targets the PTEN/Akt/mTOR pathway to antagonise neuronal injury due to excessive autophagy.","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":"15 5","pages":"e70295"},"PeriodicalIF":14.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13145360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systemic Propagation of STING Signalling via Generation of Large Extracellular Vesicles. 通过大细胞外囊泡产生的STING信号的系统传播。

IF 14.5 1区医学

Journal of Extracellular Vesicles Pub Date : 2026-05-01 DOI: 10.1002/jev2.70289

Jiae Lee, Annabel Vernon, Hyung Joon Park, Young V Kwon

{"title":"Systemic Propagation of STING Signalling via Generation of Large Extracellular Vesicles.","authors":"Jiae Lee, Annabel Vernon, Hyung Joon Park, Young V Kwon","doi":"10.1002/jev2.70289","DOIUrl":"https://doi.org/10.1002/jev2.70289","url":null,"abstract":"Large extracellular vesicles (EVs) derived from tumour cells play important roles in tumour formation and progression. However, it remains unclear why malignant cells produce these EVs and how they act in vivo. We employ a well-characterized Drosophila tumour model and demonstrate that large EV biogenesis from malignant cells is an evolutionarily conserved process. Our study uncovers an essential role for the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway, which mediates the innate immune response to cytosolic DNA, in driving large EV biogenesis and inducing a systemic immune response to tumours. STING engages a signalling axis comprising JNK and FAK-independent of TANK-binding kinase 1 (TBK1) and inhibitor of nuclear factor κB kinase (IKKβ)-to drive large EV biogenesis in both Drosophila and human malignant cells. Transplantation of large EVs from Drosophila tumours to wild type larvae is sufficient to recapitulate the systemic immune response to tumours by activating STING signalling in macrophage-like cells. Thus, our study establishes a novel animal model for studying large EVs derived from malignant cells and provides insights into how STING signalling propagates from tumour cells to the immune system via large EV biogenesis, inducing a systemic immune response to tumours.","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":"15 5","pages":"e70289"},"PeriodicalIF":14.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147863979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quality Evaluation Considerations for Stem Cell-Derived Extracellular Vesicles-Based Therapeutic Products in China. 中国干细胞来源的细胞外囊泡治疗产品的质量评价考虑。

IF 14.5 1区医学

Journal of Extracellular Vesicles Pub Date : 2026-05-01 DOI: 10.1002/jev2.70297

Tao Na, Kehua Zhang, Libo Zhao, Xuanbao Yao, Chongfeng Xu, Yuan Zhang, Lan Wang, Miao Xu, Hui Zhang, Shufang Meng

{"title":"Quality Evaluation Considerations for Stem Cell-Derived Extracellular Vesicles-Based Therapeutic Products in China.","authors":"Tao Na, Kehua Zhang, Libo Zhao, Xuanbao Yao, Chongfeng Xu, Yuan Zhang, Lan Wang, Miao Xu, Hui Zhang, Shufang Meng","doi":"10.1002/jev2.70297","DOIUrl":"https://doi.org/10.1002/jev2.70297","url":null,"abstract":"Stem cell therapy is currently undergoing clinical research in China for conditions that are resistant to or incurable by traditional pharmaceutical interventions. Stem cell-derived extracellular vesicles (EVs) exhibit therapeutic effects similar to those of their parent stem cells, positioning them as an alternative or adjunctive approach to stem cell therapy. In recent years, given the tremendous potential for EVs in disease treatment, many researchers have focused on the development of stem cell-derived EVs and have achieved substantial progress in large-scale production and quality-related studies. However, at present, there are no specific or targeted regulatory requirements issued by authorities in China regarding the regulation of this novel therapeutic modality or the assurance of its safety and efficacy. In this paper, based on the biological properties of EVs, recent research advances, current understanding of their mechanisms of action, manufacturing processes and quality control strategies, a comprehensive framework for the quality evaluation of stem cell-derived EV-based therapeutic products is proposed. This framework is intended to serve as a reference for researchers and developers and may help to facilitate further discussion to facilitate further discussion, thereby supporting and promoting the development, regulatory oversight and establishment of quality standards and evaluation systems for stem cell-derived EVs in China.","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":"15 5","pages":"e70297"},"PeriodicalIF":14.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147863944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipidome Analysis of Cancer Cells and Their Extracellular Vesicles Reveals Cancer-Type-Specific Lipid Signatures and Enables the Design of EV-Mimetic Liposomes. 癌细胞及其细胞外囊泡的脂质组分析揭示了癌症类型特异性脂质特征，并使模拟ev脂质体的设计成为可能。

IF 14.5 1区医学

Journal of Extracellular Vesicles Pub Date : 2026-05-01 DOI: 10.1002/jev2.70265

Noélie Douanne, Yousra Benslimane, Rubén R López, Prisca Bustamante, Thupten Tsering, Yunxi Chen, Laura Kienzle, Masoud Ghasemi, Lorne Taylor, Yuning Chen, Anaïs Toreja Boutin, David Juncker, Catherine Mounier, Vahé Nerguizian, Julia V Burnier

{"title":"Lipidome Analysis of Cancer Cells and Their Extracellular Vesicles Reveals Cancer-Type-Specific Lipid Signatures and Enables the Design of EV-Mimetic Liposomes.","authors":"Noélie Douanne, Yousra Benslimane, Rubén R López, Prisca Bustamante, Thupten Tsering, Yunxi Chen, Laura Kienzle, Masoud Ghasemi, Lorne Taylor, Yuning Chen, Anaïs Toreja Boutin, David Juncker, Catherine Mounier, Vahé Nerguizian, Julia V Burnier","doi":"10.1002/jev2.70265","DOIUrl":"10.1002/jev2.70265","url":null,"abstract":"Lipid metabolism reprogramming is a hallmark of cancer, yet the global lipidome of cancer cells and their extracellular vesicles (EVs) remains poorly understood. Using mass spectrometry, we analyzed the lipid profiles of a panel of human cancer and non-cancer cell lines along with their secreted EVs. Cancer cells exhibited distinct lipid signatures, including elevated lipid raft components. Cancer-derived EVs displayed unique lipid compositions that clustered separately from cell lipid profiles, suggesting active lipid sorting during EV biogenesis. Comparative analysis of primary and metastatic cells and their EVs, highlighted phospholipid alterations during metastasis. These findings suggest that EV lipid profiles could serve as cancer biomarkers, and the data can inform synthetic EV-based nanoparticle design for drug delivery. Our study provides one of the most comprehensive characterizations of the cancer EV lipidome to date, offering novel insights into lipid metabolism in cancer progression and potential therapeutic applications.","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":"15 5","pages":"e70265"},"PeriodicalIF":14.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13129227/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147773088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0