Journal of Extracellular Vesicles最新文献

{"title":"Thermotolerance in S. cerevisiae as a model to study extracellular vesicle biology","authors":"Curtis John Logan,&nbsp;Claire C. Staton,&nbsp;Joshua Thomas Oliver,&nbsp;Jeff Bouffard,&nbsp;Thomas David Daniel Kazmirchuk,&nbsp;Melissa Magi,&nbsp;Christopher Leonard Brett","doi":"10.1002/jev2.12431","DOIUrl":"https://doi.org/10.1002/jev2.12431","url":null,"abstract":"<p>The budding yeast <i>Saccharomyces cerevisiae</i> is a proven model organism for elucidating conserved eukaryotic biology, but to date its extracellular vesicle (EV) biology is understudied. Here, we show yeast transmit information through the extracellular medium that increases survival when confronted with heat stress and demonstrate the EV-enriched samples mediate this thermotolerance transfer. These samples contain vesicle-like particles that are exosome-sized and disrupting exosome biogenesis by targeting endosomal sorting complexes required for transport (ESCRT) machinery inhibits thermotolerance transfer. We find that Bro1, the yeast ortholog of the human exosome biomarker ALIX, is present in EV samples, and use Bro1 tagged with green fluorescent protein (GFP) to track EV release and uptake by endocytosis. Proteomics analysis reveals that heat shock protein 70 (HSP70) family proteins are enriched in EV samples that provide thermotolerance. We confirm the presence of the HSP70 ortholog stress-seventy subunit A2 (Ssa2) in EV samples and find that mutant yeast cells lacking SSA2 produce EVs but they fail to transfer thermotolerance. We conclude that Ssa2 within exosomes shared between yeast cells contributes to thermotolerance. Through this work, we advance <i>Saccharomyces cerevisiae</i> as an emerging model organism for elucidating molecular details of eukaryotic EV biology and establish a role for exosomes in heat stress and proteostasis that seems to be evolutionarily conserved.</p>","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":null,"pages":null},"PeriodicalIF":16.0,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jev2.12431","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140844858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to article pagination in the Journal of Extracellular Vesicles","authors":"","doi":"10.1002/jev2.12443","DOIUrl":"https://doi.org/10.1002/jev2.12443","url":null,"abstract":"<p>The following articles in the Journal of Extracellular Vesicles contained an error in pagination. The “e” was left out of the eLocator pagination code. The pagination has been updated in each article. We apologize for this error.</p><p>(2024), Correction to Heat inactivation of foetal bovine serum performed after EV-depletion influences the proteome of cell-derived extracellular vesicles. J Extracell Vesicles., 13: e12411. https://doi.org/10.1002/jev2.12411</p><p>Chen, Z., Luo, L., Ye, T., Zhou, J., Niu, X., Yuan, J., Yuan, T., Fu, D., Li, H., Li, Q., &amp; Wang, Y. (2024). Identification of specific markers for human pluripotent stem cell-derived small extracellular vesicles. Journal of Extracellular Vesicles, 13, e12409. https://doi.org/10.1002/jev2.12409</p><p>Gao, Y., Mi, N., Wu, W., Zhao, Y., Fan, F., Liao, W., Ming, Y., Guan, W., &amp; Bai, C. (2024). Transfer of inflammatory mitochondria via extracellular vesicles from M1 macrophages induces ferroptosis of pancreatic beta cells in acute pancreatitis. Journal of Extracellular Vesicles, 13, e12410. https://doi.org/10.1002/jev2.12410</p><p>Hansen, A. S., Jensen, L. S., Gammelgaard, K. R., Ryttersgaard, K. G., Krapp, C., Just, J., Jønsson, K. L., Jensen, P. B., Boesen, T., Johannsen, M., Etzerodt, A., Deleuran, B. W., &amp; Jakobsen, M. R. (2023). T-cell derived extracellular vesicles prime macrophages for improved STING based cancer immunotherapy. Journal of Extracellular Vesicles, 12, e12350. https://doi.org/10.1002/jev2.12350</p><p>Klemetti, M. M., Pettersson, A. B. V., Ahmad Khan, A., Ermini, L., Porter, T. R., Litvack, M. L., Alahari, S., Zamudio, S., Illsley, N. P., Röst, H., Post, M., &amp; Caniggia, I. (2024). Lipid profile of circulating placental extracellular vesicles during pregnancy identifies foetal growth restriction risk. Journal of Extracellular Vesicles, 13, e12413. https://doi.org/10.1002/jev2.12413</p><p>Kyykallio, H., Faria, A. V. S., Hartman, R., Capra, J., Rilla, K., &amp; Siljander, P. R.-M. (2022). A quick pipeline for the isolation of 3D cell culture-derived extracellular vesicles. Journal of Extracellular Vesicles, 11, e12273. https://doi.org/10.1002/jev2.12273</p><p>Lötvall, J. (2024), Publishing the MISEV guidelines; The editorial process. J Extracell Vesicles., 13: e12415. https://doi.org/10.1002/jev2.12415</p><p>Phu, T. A., Ng, M., Vu, N. K., Gao, A. S., &amp; Raffai, R. L. (2023). ApoE expression in macrophages communicates immunometabolic signaling that controls hyperlipidemia-driven hematopoiesis &amp; inflammation via extracellular vesicles. Journal of Extracellular Vesicles, 12, e12345. https://doi.org/10.1002/jev2.12345</p><p>Schöne, N., Kemper, M., Menck, K., Evers, G., Krekeler, C., Schulze, A. B., Lenz, G., Wardelmann, E., Binder, C., &amp; Bleckmann, A. (2024). PD-L1 on large extracellular vesicles is a predictive biomarker for therapy response in tissue PD-L1-low and -negative patients with non-small cell lung cancer. Journal of Extracellular Vesicl","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":null,"pages":null},"PeriodicalIF":16.0,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jev2.12443","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140818960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exhaled breath condensate contains extracellular vesicles (EVs) that carry miRNA cargos of lung tissue origin that can be selectively purified and analyzed","authors":"Megan I. Mitchell,&nbsp;Iddo Z. Ben-Dov,&nbsp;Kenny Ye,&nbsp;Christina Liu,&nbsp;Miao Shi,&nbsp;Ali Sadoughi,&nbsp;Chirag Shah,&nbsp;Taha Siddiqui,&nbsp;Aham Okorozo,&nbsp;Martin Gutierrez,&nbsp;Rashmi Unawane,&nbsp;Lisa Biamonte,&nbsp;Kaushal Parihk,&nbsp;Simon Spivack,&nbsp;Olivier Loudig","doi":"10.1002/jev2.12440","DOIUrl":"https://doi.org/10.1002/jev2.12440","url":null,"abstract":"<p>Lung diseases, including lung cancer, are rising causes of global mortality. Despite novel imaging technologies and the development of biomarker assays, the detection of lung cancer remains a significant challenge. However, the lung communicates directly with the external environment and releases aerosolized droplets during normal tidal respiration, which can be collected, stored and analzsed as exhaled breath condensate (EBC). A few studies have suggested that EBC contains extracellular vesicles (EVs) whose microRNA (miRNA) cargos may be useful for evaluating different lung conditions, but the cellular origin of these EVs remains unknown. In this study, we used nanoparticle tracking, transmission electron microscopy, Western blot analyses and super resolution nanoimaging (ONi) to detect and validate the identity of exhaled EVs (exh-EVs). Using our customizable antibody-purification assay, EV-CATCHER, we initially determined that exh-EVs can be selectively enriched from EBC using antibodies against three tetraspanins (CD9, CD63 and CD81). Using ONi we also revealed that some exh-EVs harbour lung-specific proteins expressed in bronchiolar Clara cells (Clara Cell Secretory Protein [CCSP]) and Alveolar Type II cells (Surfactant protein C [SFTPC]). When conducting miRNA next generation sequencing (NGS) of airway samples collected at five different anatomic levels (i.e., mouth rinse, mouth wash, bronchial brush, bronchoalveolar lavage [BAL] and EBC) from 18 subjects, we determined that miRNA profiles of exh-EVs clustered closely to those of BAL EVs but not to those of other airway samples. When comparing the miRNA profiles of EVs purified from matched BAL and EBC samples with our three tetraspanins EV-CATCHER assay, we captured significant miRNA expression differences associated with smoking, asthma and lung tumor status of our subjects, which were also reproducibly detected in EVs selectively purified with our anti-CCSP/SFTPC EV-CATCHER assay from the same samples, but that confirmed their lung tissue origin. Our findings underscore that enriching exh-EV subpopulations from EBC allows non-invasive sampling of EVs produced by lung tissues.</p>","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":null,"pages":null},"PeriodicalIF":16.0,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jev2.12440","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140641868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell-engineered virus-mimetic nanovesicles for vaccination against enveloped viruses","authors":"Chungmin Han,&nbsp;Suyeon Kim,&nbsp;Youngjoo Seo,&nbsp;Minyeob Lim,&nbsp;Yongmin Kwon,&nbsp;Johan Yi,&nbsp;Seung-Ik Oh,&nbsp;Minsu Kang,&nbsp;Seong Gyu Jeon,&nbsp;Jaesung Park","doi":"10.1002/jev2.12438","DOIUrl":"https://doi.org/10.1002/jev2.12438","url":null,"abstract":"<p>Enveloped viruses pose a significant threat to human health, as evidenced by the recent COVID-19 pandemic. Although current vaccine strategies have proven effective in preventing viral infections, the development of innovative vaccine technologies is crucial to fortify our defences against future pandemics. In this study, we introduce a novel platform called cell-engineered virus-mimetic nanovesicles (VNVs) and demonstrate their potential as a vaccine for targeting enveloped viruses. VNVs are generated by extruding plasma membrane-derived blebs through nanoscale membrane filters. These VNVs closely resemble enveloped viruses and extracellular vesicles (EVs) in size and morphology, being densely packed with plasma membrane contents and devoid of materials from other membranous organelles. Due to these properties, VNVs express viral membrane antigens more extensively and homogeneously than EVs expressing the same antigen. In this study, we produced severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) VNVs expressing the SARS-CoV-2 Spike glycoprotein (S) on their surfaces and assessed their preclinical efficacy as a COVID-19 vaccine in experimental animals. The administration of VNVs successfully stimulated the production of S-specific antibodies both systemically and locally, and immune cells isolated from vaccinated mice displayed cytokine responses to S stimulation.</p>","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":null,"pages":null},"PeriodicalIF":16.0,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jev2.12438","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140641859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to “Transfer of inflammatory mitochondria via extracellular vesicles from M1 macrophages induces ferroptosis of pancreatic beta cells in acute pancreatitis”","authors":"","doi":"10.1002/jev2.12441","DOIUrl":"https://doi.org/10.1002/jev2.12441","url":null,"abstract":"<p>Gao Y., Mi N., Wu W., Zhao Y., Fan F., Liao W., Ming Y., Guan W., &amp; Bai C. (2024). Transfer of inflammatory mitochondria via extracellular vesicles from M1 macrophages induces ferroptosis of pancreatic beta cells in acute pancreatitis. Journal of Extracellular Vesicles, 13, e12410. https://doi.org/10.1002/jev2.12410</p><p>In the originally published article, there was an error in the address of author Weijun Guan. The correct address is as follows:</p><p>Weijun Guan</p><p>Institute of Animal Sciences, Chinese Academy of Agricultural Sciences</p><p>Yuanmingyuan West Road, Haidian District</p><p>Beijing 100193, R.P. China</p><p>Email: <span>[email protected]</span></p><p>This information has been updated in the online version of the article.</p><p>We apologize for this error.</p>","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":null,"pages":null},"PeriodicalIF":16.0,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jev2.12441","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140632081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuropathogenic role of astrocyte-derived extracellular vesicles in HIV-associated neurocognitive disorders","authors":"Divya T. Chemparathy,&nbsp;Sudipta Ray,&nbsp;Chase Ochs,&nbsp;Natasha Ferguson,&nbsp;Dinesh Y. Gawande,&nbsp;Shashank M. Dravid,&nbsp;Shannon Callen,&nbsp;Susmita Sil,&nbsp;Shilpa Buch","doi":"10.1002/jev2.12439","DOIUrl":"https://doi.org/10.1002/jev2.12439","url":null,"abstract":"<p>Our previous findings demonstrated that astrocytic HIF-1α plays a major role in HIV-1 Tat-mediated amyloidosis which can lead to Alzheimer's-like pathology-a comorbidity of HIV-Associated Neurocognitive Disorders (HAND). These amyloids can be shuttled in extracellular vesicles, and we sought to assess whether HIV-1 Tat stimulated astrocyte-derived EVs (ADEVs) containing the toxic amyloids could result in neuronal injury in vitro and in vivo. We thus hypothesized that blocking HIF-1α could likely mitigate HIV-1 Tat-ADEV-mediated neuronal injury. Rat hippocampal neurons when exposed to HIV-1 Tat-ADEVs carrying the toxic amyloids exhibited amyloid accumulation and synaptodendritic injury, leading to functional loss as evidenced by alterations in miniature excitatory post synaptic currents. The silencing of astrocytic HIF-1α not only reduced the biogenesis of ADEVs, as well as amyloid cargos, but also ameliorated neuronal synaptodegeneration. Next, we determined the effect of HIV-1 Tat-ADEVs carrying amyloids in the hippocampus of naive mice brains. Naive mice receiving the HIV-1 Tat-ADEVs, exhibited behavioural changes, and Alzheimer's ’s-like pathology accompanied by synaptodegeneration. This impairment(s) was not observed in mice injected with HIF-1α silenced ADEVs. This is the first report demonstrating the role of amyloid-carrying ADEVs in mediating synaptodegeneration leading to behavioural changes associated with HAND and highlights the protective role of HIF-1α.</p>","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":null,"pages":null},"PeriodicalIF":16.0,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jev2.12439","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140632082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Membranes on the move: The functional role of the extracellular vesicle membrane for contact-dependent cellular signalling","authors":"Kevin Jahnke,&nbsp;Oskar Staufer","doi":"10.1002/jev2.12436","DOIUrl":"https://doi.org/10.1002/jev2.12436","url":null,"abstract":"<p>Extracellular vesicles (EVs), lipid-enclosed structures released by virtually all life forms, have gained significant attention due to their role in intercellular and interorganismal communication. Despite their recognized importance in disease processes and therapeutic applications, fundamental questions about their primary function remain. Here, we propose a different perspective on the primary function of EVs, arguing that they serve as essential elements providing membrane area for long-distance, contact-dependent cellular communication based on protein-protein interaction. While EVs have been recognized as carriers of genetic information, additional unique advantages that they could provide for cellular communication remain unclear. Here, we introduce the concept that the substantial membrane area provided by EVs allows for membrane contact-dependent interactions that could be central to their function. This membrane area enables the lateral diffusion and sorting of membrane ligands like proteins, polysaccharides or lipids in two dimensions, promoting avidity-driven effects and assembly of co-stimulatory architectures at the EV-cell interface. The concept of vesicle-induced receptor sequestration (VIRS), for example, describes how EVs confine and focus receptors at the EV contact site, promoting a dense local concentration of receptors into signalosomes. This process can increase the signalling strength of EV-presented ligands by 10-1000-fold compared to their soluble counterparts. The speculations in this perspective advance our understanding of EV-biology and have critical implications for EV-based applications and therapeutics. We suggest a shift in perspective from viewing EVs merely as transporters of relevant nucleic acids and proteins to considering their unique biophysical properties as presentation platforms for long-distance, contact-dependent signalling. We therefore highlight the functional role of the EV membrane rather than their content. We further discuss how this signalling mechanism might be exploited by virus-transformed or cancer cells to enhance immune-evasive mechanisms.</p>","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":null,"pages":null},"PeriodicalIF":16.0,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jev2.12436","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140633711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EV-COMM: A database of interspecies and intercellular interactions mediated by extracellular vesicles","authors":"Jingyu Chen,&nbsp;Jing-Jing Lin,&nbsp;Weiyi Wang,&nbsp;Haining Huang,&nbsp;Zhizhen Pan,&nbsp;Guozhu Ye,&nbsp;Sijun Dong,&nbsp;Yi Lin,&nbsp;Congtian Lin,&nbsp;Qiansheng Huang","doi":"10.1002/jev2.12442","DOIUrl":"https://doi.org/10.1002/jev2.12442","url":null,"abstract":"<p>Intra- and inter-organismal interactions play a crucial role in the maintenance and function of individuals, as well as communities. Extracellular vesicles (EVs) have been identified as effective mediators for the communication both within and between species. They can carry and transport molecular cargoes to transmit biological messages. Several databases (ExoBCD, ExoCarta, EVpedia, EV-TRACK, Vesiclepedia) complied the cargoes information including DNA, RNA, protein, lipid and metabolite associated with EVs. Databases that refer to the complete records on both donor and recipient information are warranted to facilitate the understanding of the interaction across cells and species. In this study, we developed a database that compiled the records equipped with a structured process of EV-mediated interaction. The sources of donor and recipient were classified by cell type, tissues/organs and species, thus providing an extended knowledge of cell-cell, species-species interaction. The isolation and identification methods were presented for assessing the quality of EVs. Information on functional cargoes was included, where microRNA was linked to a prediction server to broaden its potential effects. Physiological and pathological context was marked to show the environment where EVs functioned. At present, a total of 1481 data records in our database, including 971 cell-cell interactions belonging to more than 40 different tissues/organs, and 510 cross-species records. The database provides a web interface to browse, search, visualize and download the interaction records. Users can search for interactions by selecting the context of interest or specific cells/species types, as well as functional cargoes. To the best of our knowledge, the database is the first comprehensive database focusing on interactions between donor and recipient cells or species mediated by EVs, serving as a convenient tool to explore and validate interactions. The Database, shorten as EV-COMM (EV mediated communication) is freely available at http://sdc.iue.ac.cn/evs/list/ and will be continuously updated.</p>","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":null,"pages":null},"PeriodicalIF":16.0,"publicationDate":"2024-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jev2.12442","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140632011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional diversity of apoptotic vesicle subpopulations from bone marrow mesenchymal stem cells in tissue regeneration","authors":"Xuanhao Zhang,&nbsp;Jian Yang,&nbsp;Shixing Ma,&nbsp;Xin Gao,&nbsp;Guanyu Wang,&nbsp;Yanping Sun,&nbsp;Yejia Yu,&nbsp;Zhuo Wang,&nbsp;Weidong Tian,&nbsp;Li Liao","doi":"10.1002/jev2.12434","DOIUrl":"https://doi.org/10.1002/jev2.12434","url":null,"abstract":"<p>Apoptosis releases numerous apoptotic vesicles that regulate processes such as cell proliferation, immunity, and tissue regeneration and repair. Now, it has also emerged as an attractive candidate for biotherapeutics. However, apoptotic vesicles encompass a diverse range of subtypes, and it remains unclear which specific subtypes play a pivotal role. In this study, we successfully isolated different apoptotic vesicle subtypes based on their sizes and characterized them using NTA and TEM techniques, respectively. We compared the functional variances among the distinct subtypes of apoptotic vesicles in terms of stem cell proliferation, migration, and differentiation, as well as for endothelial cell and macrophage function, effectively identifying subtypes that exhibit discernible functional differences. ApoSEV (with diameter &lt;1000 nm) promoted stem cell proliferation, migration, and multi-potent differentiation, and accelerated skin wound healing of diabetes mouse model, while apoBD (with diameter &gt;1000 nm) played the opposite effect on cell function and tissue regeneration. Lastly, employing protein analysis and gene sequencing techniques, we elucidated the intrinsic mechanisms underlying these differences between different subtypes of apoEVs. Collectively, this study identified that apoptotic vesicle subtypes possessed distinct bio-functions in regulating stem cell function and behaviour and modulating tissue regeneration, which primarily attribute to the distinct profiling of protein and mRNA in different subtypes. This comprehensive analysis of specific subtypes of apoEVs would provide novel insights for potential therapeutic applications in cell biology and tissue regeneration.</p>","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":null,"pages":null},"PeriodicalIF":16.0,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jev2.12434","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140606429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neonatal enteroids absorb extracellular vesicles from human milk-fed infant digestive fluid","authors":"Claire Yung,&nbsp;Yang Zhang,&nbsp;Madeline Kuhn,&nbsp;Randall J. Armstrong,&nbsp;Amy Olyaei,&nbsp;Molly Aloia,&nbsp;Brian Scottoline,&nbsp;Sarah F. Andres","doi":"10.1002/jev2.12422","DOIUrl":"https://doi.org/10.1002/jev2.12422","url":null,"abstract":"<p>Human milk contains extracellular vesicles (HMEVs). Pre-clinical models suggest that HMEVs may enhance intestinal function and limit inflammation; however, it is unknown if HMEVs or their cargo survive neonatal human digestion. This limits the ability to leverage HMEV cargo as additives to infant nutrition or as therapeutics. This study aimed to develop an EV isolation pipeline from small volumes of human milk and neonatal intestinal contents after milk feeding (digesta) to address the hypothesis that HMEVs survive in vivo neonatal digestion to be taken up intestinal epithelial cells (IECs). Digesta was collected from nasoduodenal sampling tubes or ostomies. EVs were isolated from raw and pasteurized human milk and digesta by density-gradient ultracentrifugation following two-step skimming, acid precipitation of caseins, and multi-step filtration. EVs were validated by electron microscopy, western blotting, nanoparticle tracking analysis, resistive pulse sensing, and super-resolution microscopy. EV uptake was tested in human neonatal enteroids. HMEVs and digesta EVs (dEVs) show typical EV morphology and are enriched in CD81 and CD9, but depleted of β-casein and lactalbumin. HMEV and some dEV fractions contain mammary gland-derived protein BTN1A1. Neonatal human enteroids rapidly take up dEVs in part via clathrin-mediated endocytosis. Our data suggest that EVs can be isolated from digestive fluid and that these dEVs can be absorbed by IECs.</p>","PeriodicalId":15811,"journal":{"name":"Journal of Extracellular Vesicles","volume":null,"pages":null},"PeriodicalIF":16.0,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jev2.12422","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140544412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}