Journal of Experimental Biology最新文献_第9页

Albumin is ubiquitously expressed in the dolphin body and upregulated by an extracellular albumin shortage. 白蛋白在海豚体内普遍表达，并因细胞外白蛋白缺乏而上调。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-15 Epub Date: 2025-05-22 DOI: 10.1242/jeb.249752

Kaho Saito, Fumiki Hanafusa, Daiki Inamori, Takuya Itou, Miwa Suzuki

{"title":"Albumin is ubiquitously expressed in the dolphin body and upregulated by an extracellular albumin shortage.","authors":"Kaho Saito, Fumiki Hanafusa, Daiki Inamori, Takuya Itou, Miwa Suzuki","doi":"10.1242/jeb.249752","DOIUrl":"10.1242/jeb.249752","url":null,"abstract":"This study demonstrates that hepatocyte-specific albumin is ubiquitously expressed and the protein is produced in both common bottlenose dolphins Tursiops truncatus and striped dolphins Stenella coeruleoalba. Quantitative PCR revealed that albumin precursor mRNA is expressed in all 12 tissues examined, with the highest levels observed in the liver. Next, TA cloning using skin and liver of the two species confirmed that the deduced albumin animo acid sequences in the tissues are 100% identical within each species and slightly different between the species or between tissues in common bottlenose dolphin and cultured cells derived from the same species. In addition, western blot analysis showed positive bands of approximately 66 kDa in all tissues examined, as seen with serum albumin. Immunohistochemistry assays also revealed that albumin protein was distributed in most cells in each tissue. These results suggest that albumin protein, with the same sequence as the serum albumin synthesized in liver, may be ubiquitously expressed in the dolphin body. Furthermore, to clarify the function of albumin, we removed bovine serum albumin from culture medium. This enhanced albumin mRNA expression in the cells. Albumin protein with the specific dolphin sequence then began to be detected in the medium. These data strongly indicate that albumin may be synthesized in many tissue types and extracellularly secreted in the same manner as in the liver. Additionally, albumin expression is likely to be upregulated in the absence of albumin around the cells. Ubiquitous albumin expression may be essential for dolphin peripheral cells, possibly to supply albumin during dive-induced ischemia.","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12148036/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143984983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optic flow, a rich source of optic information for harbour seals (Phoca vitulina). 光流是斑海豹（Phoca vitulina）丰富的光信息来源。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-15 Epub Date: 2025-05-29 DOI: 10.1242/jeb.250168

Laura-Marie Sandow, Ann-Kathrin Thimian, Markus Lappe, Frederike D Hanke

{"title":"Optic flow, a rich source of optic information for harbour seals (Phoca vitulina).","authors":"Laura-Marie Sandow, Ann-Kathrin Thimian, Markus Lappe, Frederike D Hanke","doi":"10.1242/jeb.250168","DOIUrl":"10.1242/jeb.250168","url":null,"abstract":"Marine mammal vision is often considered to only provide limited information, particularly underwater in low light levels and turbidity. However, when these animals move through turbid water optic flow is elicited. A past study has documented the harbour seal's (Phoca vitulina) ability to perceive deviations from heading from optic flow simulating movement through a volume of turbid water. Here, we asked whether harbour seals are also able to perceive and analyse surface optic flow. Thus, we simulated three optic flow environments and trained three harbour seals to determine the simulated heading. The harbour seals precisely indicated their heading with a mean (±s.d.) accuracy of 4.61±0.56 deg for volume optic flow, 4.96±0.74 deg for surface optic flow mimicking movement over a surface and 3.58±1.12 deg for surface optic flow mimicking movement underneath a surface. We conclude that harbour seals have access to and can thus rely on optic (flow) information whenever there is enough light for vision, thus refuting existing opinions about poor visual guidance in harbour seals or, more generally, in marine mammals. A detailed analysis of optic flow perception in (semi-) aquatic animals is expected to enhance our understanding of optic flow perception and vision in general.","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":"228 10","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12148030/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modelling endurance in free-ranging animals using tracking collars: insights from domestic hunting dogs. 用追踪项圈模拟自由放养动物的耐力：来自家养猎犬的见解。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-15 Epub Date: 2025-05-21 DOI: 10.1242/jeb.250259

Pablo Rozier-Delgado, Mathieu Garel, Cyril Rousset, Anne Loison, Baptiste Morel

引用次数: 0

I can't hear you: effects of noise on auditory processing in mixed-species flocks. 我听不见你：鸣禽在噪音中的听觉处理。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-15 Epub Date: 2025-05-22 DOI: 10.1242/jeb.250033

Trina L Chou, Megan D Gall

{"title":"I can't hear you: effects of noise on auditory processing in mixed-species flocks.","authors":"Trina L Chou, Megan D Gall","doi":"10.1242/jeb.250033","DOIUrl":"10.1242/jeb.250033","url":null,"abstract":"Animals have evolved complex auditory systems to extract acoustic information from natural environmental noise, yet they are challenged by rising levels of novel anthropogenic noise. Songbirds adjust their vocal production in response to increasing noise, but auditory processing of signals in noise remains understudied. Auditory processing characteristics, including auditory filter bandwidth, filter efficiency and critical ratios (level-independent signal-to-noise ratios at threshold), likely influence auditory and behavioral responses to noise. Here, we investigated the effects of noise on auditory processing in three songbird species (black-capped chickadees, tufted titmice and white-breasted nuthatches) that live in mixed-species flocks and rely on heterospecific communication to coordinate mobbing behaviors. We determined masked thresholds and critical ratios from 1 to 4 kHz using auditory evoked potentials. We predicted that nuthatches would have the lowest critical ratios given that they have narrowest filters, followed by titmice and then chickadees. We found that nuthatches had the greatest sensitivity in quiet conditions, but the highest critical ratios, suggesting their auditory sensitivity is highly susceptible to noise. Titmice had the lowest critical ratios, suggesting relatively minor impacts of noise on their auditory processing. This is not consistent with predictions based on auditory filter bandwidth, but is consistent with both recent behavioral findings and predictions made by auditory filter efficiency measures. Detrimental effects of noise were most prevalent in the 2-4 kHz range, frequencies produced in vocalizations. Our results using the critical ratio as a measure of processing in noise suggest that low levels of anthropogenic noise may influence these three species differently.","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12148034/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Foldable insect wings: from folding and unfolding mechanisms to inspired applications. 可折叠昆虫翅膀：从折叠和展开机制到灵感应用。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-15 Epub Date: 2025-05-27 DOI: 10.1242/jeb.249706

Qinzhou Luo, Fanan Wei, Wei Liu

引用次数: 0

Ecosystem engineers on tropical reefs in transition: giant barrel sponges in the Anthropocene. 热带珊瑚礁转型中的生态系统工程师：人类世的巨桶海绵。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-15 Epub Date: 2025-05-13 DOI: 10.1242/jeb.250082

Joseph R Pawlik

{"title":"Ecosystem engineers on tropical reefs in transition: giant barrel sponges in the Anthropocene.","authors":"Joseph R Pawlik","doi":"10.1242/jeb.250082","DOIUrl":"https://doi.org/10.1242/jeb.250082","url":null,"abstract":"Tropical coral reef ecosystems are changing rapidly to an alternative state in which sponges are the dominant living habitat, with giant barrel sponges (GBSs, Xestospongia spp.) representing the largest biomass. Unlike other benthic reef organisms, GBSs are ecosystem engineers that pump large volumes of seawater, disrupting the benthic boundary layer and directing flow away from the reef surface and into the water column. The morphology and size of GBSs have made them particularly good experimental subjects to study the hydraulics of sponge pumping and the transformation that occurs as seawater is processed by the sponge holobiont (sponge cells and microbial symbionts). This Review is part of a series marking the 100th birthday of The Company of Biologists, which was founded by marine biologist George Parker Bidder III, who primarily worked on sponges. The Review provides an integrative assessment of research on GBSs with comparisons with what is known about other marine sponges. Recent discoveries suggest that ancient lineages of morphologically indistinguishable GBSs are responding to environmental changes over sub-decadal time periods to rapidly populate reefs stripped of coral cover by climate change. If GBSs remain robust to rising seawater temperatures, they will become the greatest source of habitat complexity on reefs of the future, so knowledge of their biology and physiology will be important to our understanding of these ecosystems.","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":"228 10","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between joint shape and function as revealed through ex vivo XROMM. 通过体外 XROMM 揭示关节形状与功能之间的关系。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-15 Epub Date: 2025-05-16 DOI: 10.1242/jeb.249261

Robert J Brocklehurst, L Fahn-Lai, Andrew Biewener, Stephanie E Pierce

{"title":"Relationship between joint shape and function as revealed through ex vivo XROMM.","authors":"Robert J Brocklehurst, L Fahn-Lai, Andrew Biewener, Stephanie E Pierce","doi":"10.1242/jeb.249261","DOIUrl":"10.1242/jeb.249261","url":null,"abstract":"Skeletal joint morphology and mobility underlie movement, behavior and ecology in vertebrates. Joints can be categorized by their shape and articulation type, but such schemes might be unreliable for inferring function across the full diversity of vertebrates. We test hypothesized relationships between joint form and function by collecting marker-based ex vivo, cadaveric XROMM data on the shoulder and elbow joints of the tegu lizard (Salvator merianae) and Virginia opossum (Didelphis virginiana), which between them contain articulations historically classified as ball-and-socket, hemi-sellar, hinge and condylar joints. We measured 3D rotational and translational mobility at each joint and compared our experimental results against predictions based on articular morphology. Contrary to our predictions, the opossum ball-and-socket shoulder joint was less mobile - it had a smaller 3D range of motion envelope - than the tegu hemi-sellar shoulder joint and even the tegu condylar elbow joint, challenging the notion that ball-and-socket joints provide an inherent mobility advantage. However, the ball-and-socket opossum shoulder also had a less complex mobility envelope, with fewer interactions between degrees of freedom, allowing it to transition between poses more easily. Matching osteological predictions, the hinge elbow of the opossum was the least mobile. All joints exhibited coupling between rotational and translational degrees of freedom, further emphasizing the need to incorporate translational motion and soft tissue constraints for accurately modeling joint mobility. Our findings underscore the complexity of form-function relationships in vertebrate skeletal joints, and demonstrate that joint morphology alone, in the absence of soft tissues, does not provide a complete picture of joint mobility.","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tapered insect (Acheta domesticus) antennae have rapid damped return with minimal oscillation after perturbation. 锥形昆虫（Acheta domesticus）的触角在扰动后具有最小振荡的快速阻尼返回。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-01 Epub Date: 2025-05-06 DOI: 10.1242/jeb.249243

Marlo G McCarter, Derek Kellogg, Stanley Sowy, Catherine Loudon

{"title":"Tapered insect (Acheta domesticus) antennae have rapid damped return with minimal oscillation after perturbation.","authors":"Marlo G McCarter, Derek Kellogg, Stanley Sowy, Catherine Loudon","doi":"10.1242/jeb.249243","DOIUrl":"10.1242/jeb.249243","url":null,"abstract":"As tactile sensors, antennae must be flexible and responsive while maintaining shape and control of the structure. We evaluated the geometric and mechanical properties of cricket antennae, which we treat as bending cantilever beams. Flexural rigidity (EI) is the mechanical property that most significantly controls bending behavior. We determined that the flexural rigidity decreases steeply (proximal to distal) by evaluating the quasistatic bent shapes in response to obstacle contact at different points along the antennae. This steep decrease in flexural rigidity causes the antennae to bend readily only near the obstacle contact, in contrast to the curvature of a beam with uniform properties and cross-section (which bends closer to the base). This flexural rigidity gradient in the antennae is consistent with the morphology: a decreasing second moment of area calculated from the measured taper and the diminishing wall (cuticle) thickness. Cricket antennae recovered from a single localized perturbation quickly and with minimal to no oscillation, suggesting behavior close to critical damping (fastest return without oscillations). Bending primarily occurred in the portion of the flagellum near the obstacle contact, reducing the length of the flagellum that participated in the oscillating behavior (natural frequency ∼11 Hz). Forced sinusoidal vibrations generated a resonance frequency of ∼30 Hz with imperceptible movement in the proximal part of the flagellum while the distal part vibrated. The results suggest that tapering of an elongated mechanosensor may facilitate a rapid return to its original shape without oscillation, which is an advantageous attribute that may also inform biomimetic applications.","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":"228 9","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12079660/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of hypoxia-reoxygenation on the bioenergetics and oxidative stress in the isolated mitochondria of the king scallop, Pecten maximus. 缺氧-复氧对大扇贝离体线粒体能量学和氧化应激的影响。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-01 Epub Date: 2025-05-12 DOI: 10.1242/jeb.249870

Linda Lumor, Christian Bock, Felix Christopher Mark, Siriluck Ponsuksili, Inna Sokolova

{"title":"Effects of hypoxia-reoxygenation on the bioenergetics and oxidative stress in the isolated mitochondria of the king scallop, Pecten maximus.","authors":"Linda Lumor, Christian Bock, Felix Christopher Mark, Siriluck Ponsuksili, Inna Sokolova","doi":"10.1242/jeb.249870","DOIUrl":"10.1242/jeb.249870","url":null,"abstract":"The king scallop (Pecten maximus) is a highly aerobic subtidal bivalve species vulnerable to fluctuations in oxygen availability. This study investigated the effects of short-term (15 min) and long-term (90 min) hypoxia-reoxygenation (H/R) stress on substrate-specific mitochondrial functions in the gill and digestive gland tissues of P. maximus, oxidizing substrates that engage mitochondrial Complex I (pyruvate, palmitate) and Complex II (succinate). Under normoxic conditions, scallop mitochondria preferentially oxidized pyruvate. H/R stress induced a significant decline in Complex I-driven ATP synthesis, increased proton leak and dysregulated fatty acid oxidation, indicating mitochondrial vulnerability to H/R stress. Following H/R, both tissues demonstrated a greater capacity for succinate oxidation than for Complex I substrates; however, long-term H/R exposure led to a reduction in respiratory coupling efficiency across all substrates. Notably, gill mitochondria exhibited more effective regulation of reactive oxygen species efflux and electron leak compared with digestive gland mitochondria under H/R stress. Despite these physiological changes, no evidence of oxidative damage was detected, suggesting the presence of a robust mitochondrial antioxidant defense. Collectively, these findings suggest that succinate oxidation plays an important role in stress recovery in P. maximus, providing insights into mitochondrial resilience and the management of oxidative stress during intermittent hypoxia.","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":"228 9","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12091870/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seminal vesicle protein caCA12 in Corydoras aeneus inhibits sperm motility for sperm drinking. 凤尾蝶精囊蛋白caCA12对饮精后精子运动的抑制作用。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-01 Epub Date: 2025-05-15 DOI: 10.1242/jeb.250293

Junki Yoshida, Maho Yamamoto, Junki Kamiya, Akinaga Kondo, Yukihito Sakaguchi, Nanami Morino, Takako Saito, Masanori Kohda, Satoshi Awata, Ban Sato, Kenji Miyado, Natsuko Kawano

{"title":"Seminal vesicle protein caCA12 in Corydoras aeneus inhibits sperm motility for sperm drinking.","authors":"Junki Yoshida, Maho Yamamoto, Junki Kamiya, Akinaga Kondo, Yukihito Sakaguchi, Nanami Morino, Takako Saito, Masanori Kohda, Satoshi Awata, Ban Sato, Kenji Miyado, Natsuko Kawano","doi":"10.1242/jeb.250293","DOIUrl":"10.1242/jeb.250293","url":null,"abstract":"Seminal vesicle (SV) secretions enhance fertilization by regulating sperm motility and fertilization capacity, and by forming plugs that prevent mating with other males. Although SVs are rare in teleosts, certain species, such as Corydoras spp., do possess them. In Corydoras spp. and other species that exhibit sperm drinking or related behaviors, females attach their mouths to the males' genital pore to ingest semen, a reproductive behavior known as sperm drinking. However, the major proteins and functions of seminal vesicle fluid (SVF) in Corydoras remain unidentified. This study aimed to identify the SVF proteins in Corydoras aeneus and clarify the functions of the identified major SVF proteins. The SVF of this species was found to be highly viscous with a high protein concentration. Sperm motility was strongly suppressed in the presence of the SVF. We identified three SVF proteins - alpha-2-macroglobulin (A2M), carbonic anhydrase 12 (CA12) and lymphocyte antigen 6 (Ly6) - through RNA sequencing (RNA-Seq), LC-MS/MS and amino acid sequencing. Additionally, we found that the identified CA12, termed 'caCA12,' was degraded into about 10 kDa and 33 kDa polypeptides containing the CA domain. The 33 kDa polypeptide with the CA domain was found to inhibit sperm motility. The identified SVF proteins, including caCA12, may play a role in keeping sperm in an immotile state until they are close to the female ova, facilitating the remarkable sperm drinking reproductive process observed in C. aeneus.","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":"228 9","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12091868/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0