Journal of Electron Spectroscopy and Related Phenomena最新文献_第6页

Quantitative mapping of calcium cell reservoirs in cyanobacteria at the submicrometer scale 亚微米级蓝藻钙细胞库的定量图谱

IF 1.9 4区物理与天体物理

Journal of Electron Spectroscopy and Related Phenomena Pub Date : 2023-08-01 DOI: 10.1016/j.elspec.2023.147369

Benzerara Karim , Görgen Sigrid , Khan Monis Athar , Chauvat Franck , March Katia , Menguy Nicolas , Mehta Neha , Skouri-Panet Fériel , Swaraj Sufal , Travert Cynthia , Cassier-Chauvat Corinne , Duprat Elodie

{"title":"Quantitative mapping of calcium cell reservoirs in cyanobacteria at the submicrometer scale","authors":"Benzerara Karim , Görgen Sigrid , Khan Monis Athar , Chauvat Franck , March Katia , Menguy Nicolas , Mehta Neha , Skouri-Panet Fériel , Swaraj Sufal , Travert Cynthia , Cassier-Chauvat Corinne , Duprat Elodie","doi":"10.1016/j.elspec.2023.147369","DOIUrl":"10.1016/j.elspec.2023.147369","url":null,"abstract":"<div><p><span>Calcium plays an important role in the physiology of bacterial cells and as a free soluble form inside the cells, it is tightly regulated at a low concentration. However, much higher amounts of stored calcium exist within cells and can be remobilized at diverse occasions. As a consequence, there has been a variety of techniques developed to quantitatively map the different forms of Ca in cells. Here we show how scanning transmission x-ray microscopy (STXM), a synchrotron-based microscopy technique, conducted in the soft x-ray range at the Ca L</span><sub>2,3</sub> edges (340–360 eV) offers an original and informative quantitative view of different Ca reservoirs in bacterial cells at a spatial resolution better than 100 nm. To illustrate this, we analyzed mutants of the cyanobacterium <em>Synechococcus elongatus</em> PCC 7942, overexpressing different versions of a gene called <em>ccyA</em> from two phylogenetically distant cyanobacteria: <em>Gloeomargarita lithophora</em> and <em>Synechococcus</em><span> sp. PCC 6312. This gene is diagnostic of the capability of some cyanobacteria to form intracellular amorphous calcium carbonate (iACC) but its function remains unknown. Here we show that the overexpression of the </span><em>ccyA</em> gene in the iACC non-forming cyanobacterium <em>S. elongatus</em> PCC 7942 results in an increased Ca content, especially in some Ca-rich cells. Moreover, we show that STXM can discriminate four different reservoirs of Ca in cyanobacteria and provides quantitative assessment of their relative importance.</p></div>","PeriodicalId":15726,"journal":{"name":"Journal of Electron Spectroscopy and Related Phenomena","volume":"267 ","pages":"Article 147369"},"PeriodicalIF":1.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42195349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Dissociative electron attachment to 1- and 9-chloroanthracene in the gas phase 气相中1-和9-氯蒽的离解电子附着

IF 1.9 4区物理与天体物理

Journal of Electron Spectroscopy and Related Phenomena Pub Date : 2023-08-01 DOI: 10.1016/j.elspec.2023.147383

Nail L. Asfandiarov, Mars V. Muftakhov, Stanislav A. Pshenichnyuk

{"title":"Dissociative electron attachment to 1- and 9-chloroanthracene in the gas phase","authors":"Nail L. Asfandiarov, Mars V. Muftakhov, Stanislav A. Pshenichnyuk","doi":"10.1016/j.elspec.2023.147383","DOIUrl":"10.1016/j.elspec.2023.147383","url":null,"abstract":"<div><p><span>Dissociative electron attachment<span> (DEA) to 1-chloroanthracene and 9-chloroanthracene was investigated under gas-phase conditions. In both compounds, the elimination of the chlorine anion is the dominant channel for the dissociation of molecular negative ions (NIs). The second most intense channel leads to the formation of molecular anions (Mˉ). The autodetachment lifetime of Mˉ was measured to be about 170 μs for both compounds. The widths of the Mˉ peaks indicate that molecular anions are formed via two resonances: at thermal </span></span>electron energies<span><span> and through a shape resonance at the energy of ∼0.5 eV. Adiabatic electron affinities were estimated in the framework of the simple Arrhenius model to be 0.86 eV for both molecules, the values being close to the theoretical predictions by </span>DFT method of 0.90 eV and 0.93 eV for 1-chloroanthracene and 9-chloroanthracene respectively. Metastable negative ions are observed in the DEA spectra of both molecules, which testifies that the elimination of chlorine anions from molecular NIs appears on a time scale of several microseconds.</span></p></div>","PeriodicalId":15726,"journal":{"name":"Journal of Electron Spectroscopy and Related Phenomena","volume":"267 ","pages":"Article 147383"},"PeriodicalIF":1.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45428776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Soft X-rays radiation damage on plunge-frozen and freeze-dried maize roots evaluated by FTIR spectromicroscopy 用傅里叶红外光谱显微镜研究了软x射线辐射对浸冻和冻干玉米根系的损伤

IF 1.9 4区物理与天体物理

Journal of Electron Spectroscopy and Related Phenomena Pub Date : 2023-08-01 DOI: 10.1016/j.elspec.2023.147384

A. Gianoncelli , M. Žižić , V. Bonanni , G. Birarda , D.E. Bedolla , K. Vogel Mikuš

引用次数: 0

Preface – Advances in photoelectron diffraction and photoelectron holograph 前言:光电子衍射和光电子全息术的研究进展

IF 1.9 4区物理与天体物理

Journal of Electron Spectroscopy and Related Phenomena Pub Date : 2023-08-01 DOI: 10.1016/j.elspec.2023.147339

Hiroshi Daimon , Michel A. Van Hove

引用次数: 0

Network formation and differentiation of chitosan–acrylic acid hydrogels using X-ray absorption spectroscopy and multivariate analysis of Fourier transform infrared spectra 壳聚糖-丙烯酸水凝胶的x射线吸收光谱和傅立叶变换红外光谱多元分析

IF 1.9 4区物理与天体物理

Journal of Electron Spectroscopy and Related Phenomena Pub Date : 2023-08-01 DOI: 10.1016/j.elspec.2023.147372

Kathrina Lois M. Taaca , Hideki Nakajima , Kanjana Thumanu , Eloise I. Prieto , Magdaleno R. Vasquez Jr.

{"title":"Network formation and differentiation of chitosan–acrylic acid hydrogels using X-ray absorption spectroscopy and multivariate analysis of Fourier transform infrared spectra","authors":"Kathrina Lois M. Taaca , Hideki Nakajima , Kanjana Thumanu , Eloise I. Prieto , Magdaleno R. Vasquez Jr.","doi":"10.1016/j.elspec.2023.147372","DOIUrl":"10.1016/j.elspec.2023.147372","url":null,"abstract":"<div><p><span>Chitosan–acrylic acid (Cs–AA) hydrogels were prepared by blending different Cs concentrations and AA:Cs ratios. The Cs–AA hydrogel samples were analyzed using synchrotron radiation-induced near edge X-ray absorption fine structure (NEXAFS) and Fourier transform infrared (FTIR) spectroscopies. The characteristics of Cs were observed through the C, O, and N K edges in NEXAFS. Significant shifts in the Cs characteristic features were induced by the addition of AA. The NEXAFS findings confirmed the new covalent and ionic bridges to form the network of the Cs–AA hydrogel. Raw FTIR spectra<span> were used to investigate the different functional groups of each of the macromolecules. The study attempted to discriminate the Cs–AA hydrogels prepared with different Cs wt% concentration and AA:Cs ratio using multivariate approaches. The preprocessed dataset was evaluated using principal component analysis (PCA) and hierarchical cluster analysis (HCA). The PCA and HCA methods showed the possibility of discriminating the Cs–AA hydrogel samples from the control samples and with each other. The approaches further confirmed that the C</span></span><img>O groups found in the range of 1700–1800 cm<sup>−1</sup> can be used as an identifier for Cs–AA hydrogel samples prepared using different solution parameters. The use of FTIR coupled with multivariate approaches provided a simple and reliable way to inspect the possible discrimination of Cs–AA hydrogels.</p></div>","PeriodicalId":15726,"journal":{"name":"Journal of Electron Spectroscopy and Related Phenomena","volume":"267 ","pages":"Article 147372"},"PeriodicalIF":1.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48788357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Scanning transmission X-ray microscopy at the Advanced Light Source 先进光源的扫描透射X射线显微镜

IF 1.9 4区物理与天体物理

Journal of Electron Spectroscopy and Related Phenomena Pub Date : 2023-08-01 DOI: 10.1016/j.elspec.2023.147381

Thomas Feggeler , Abraham Levitan , Matthew A. Marcus , Hendrik Ohldag , David A. Shapiro

{"title":"Scanning transmission X-ray microscopy at the Advanced Light Source","authors":"Thomas Feggeler , Abraham Levitan , Matthew A. Marcus , Hendrik Ohldag , David A. Shapiro","doi":"10.1016/j.elspec.2023.147381","DOIUrl":"10.1016/j.elspec.2023.147381","url":null,"abstract":"<div><p>Over 50 years of development, synchrotron based X-ray microscopy has become a routine and powerful tool for the analysis of nanoscale structure and chemistry in many areas of science. Scanning X-ray microscopy is particularly well suited to the study of chemical and magnetic states of matter and has become available at most synchrotron light sources using a variety of optical schemes, detectors and sample environments. The Advanced Light Source at Lawrence Berkeley National Laboratory has an extensive program of soft X-ray scanning microscopy which supports a broad range of scientific research using a suite of advanced tools for high spatio-temporal resolution and control of active materials. Instruments operating within an energy range between 200–2500 eV with spatial resolution down to 7 nm and sub 20 picosecond time resolution are available. These capabilities can be routinely used in combination with a variety of sample stimuli, including gas or fluid flow, temperature control from 100 to 1200 K, DC bias and pulsed or continuous microwave excitation. We present here a complete survey of our instruments, their most advanced capabilities and a perspective on how they complement each other to solve complex problems in energy, materials and environmental science.</p></div>","PeriodicalId":15726,"journal":{"name":"Journal of Electron Spectroscopy and Related Phenomena","volume":"267 ","pages":"Article 147381"},"PeriodicalIF":1.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43658604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electron scattering at high momentum transfer 高动量传递时的电子散射

IF 1.9 4区物理与天体物理

Journal of Electron Spectroscopy and Related Phenomena Pub Date : 2023-08-01 DOI: 10.1016/j.elspec.2023.147382

Maarten Vos

{"title":"Electron scattering at high momentum transfer","authors":"Maarten Vos","doi":"10.1016/j.elspec.2023.147382","DOIUrl":"10.1016/j.elspec.2023.147382","url":null,"abstract":"<div><p>Energetic (multiple keV) electrons scattering at high momentum transfer (i.e. scatter over a large angle (<span><math><mrow><mo>></mo><mn>20</mn></mrow></math></span>°)) interact with a single particle, either a nucleus or a target electron. Energy transfer is then determined by the incoming energy, scattering angle and the mass and velocity of the scattering particle. When scattering from a target electron a large fraction of the incoming energy is transferred and scattered and ejected electrons can be detected in coincidence. The experiment is then known as electron momentum spectroscopy or (e,2e) spectroscopy and information about the electronic structure is obtained. When scattering from a nucleus the transferred energy is much smaller, and only the scattered electron can be detected. Then the experiment is known as electron Rutherford backscattering and one can measure the sample composition and vibrational properties. In this review we give examples of experimental results for both cases to illustrate the unique information that can be obtained by electron spectroscopy at high-momentum transfer, a less-frequently utilised experimental condition.</p></div>","PeriodicalId":15726,"journal":{"name":"Journal of Electron Spectroscopy and Related Phenomena","volume":"267 ","pages":"Article 147382"},"PeriodicalIF":1.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48011404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Present status and recent progress of research, using photoemission-electron microscopy at SPring-8 利用SPring-8光电子显微镜的研究现状及最新进展

IF 1.9 4区物理与天体物理

Journal of Electron Spectroscopy and Related Phenomena Pub Date : 2023-08-01 DOI: 10.1016/j.elspec.2023.147371

T. Ohkochi , M. Tanaka , T. Ohtsuki , Z. Horita , F. Kitajima , A. Yamaguchi , M. Kotsugi , H. Ogawa , M. Oura

引用次数: 0

Sample structure prediction from measured XPS data using Bayesian estimation and SESSA simulator 利用贝叶斯估计和SESSA模拟器对实测XPS数据进行样本结构预测

IF 1.9 4区物理与天体物理

Journal of Electron Spectroscopy and Related Phenomena Pub Date : 2023-08-01 DOI: 10.1016/j.elspec.2023.147370

Hiroshi Shinotsuka , Kenji Nagata , Malinda Siriwardana , Hideki Yoshikawa , Hayaru Shouno , Masato Okada

引用次数: 0

Development of soft X-ray ptychography and fluorescence microscopy system using total-reflection wolter mirror and application to measurement of drug-treated mammalian cells 全反射镜软X射线ptychography和荧光显微镜系统的开发及其在药物治疗哺乳动物细胞测量中的应用

IF 1.9 4区物理与天体物理

Journal of Electron Spectroscopy and Related Phenomena Pub Date : 2023-08-01 DOI: 10.1016/j.elspec.2023.147380

Yoko Takeo , Kai Sakurai , Noboru Furuya , Kyota Yoshinaga , Takenori Shimamura , Satoru Egawa , Hisao Kiuchi , Hidekazu Mimura , Haruhiko Ohashi , Yoshihisa Harada , Mari Shimura , Takashi Kimura

{"title":"Development of soft X-ray ptychography and fluorescence microscopy system using total-reflection wolter mirror and application to measurement of drug-treated mammalian cells","authors":"Yoko Takeo , Kai Sakurai , Noboru Furuya , Kyota Yoshinaga , Takenori Shimamura , Satoru Egawa , Hisao Kiuchi , Hidekazu Mimura , Haruhiko Ohashi , Yoshihisa Harada , Mari Shimura , Takashi Kimura","doi":"10.1016/j.elspec.2023.147380","DOIUrl":"10.1016/j.elspec.2023.147380","url":null,"abstract":"<div><p>We developed a soft X-ray microscopic imaging system, CARROT, that combines ptychography and fluorescence X-ray microscopy using a total-reflection Wolter mirror illumination system. The system offers advantages such as achromaticity and long working distances, enabling seamless application to a wide wavelength range. We demonstrated high-resolution imaging of cellular structures and sub-micrometer-scale changes induced by drug administration in mammalian cells. We further showcased the capability to seamlessly switch from ptychography measurements to X-ray fluorescence measurements at different wavelengths to map elemental distributions within cells. Our findings suggest that this system can be used for investigating the correlation between intracellular structures and elemental distribution, as well as for multimodal measurements of visible light fluorescence, Raman scattering, and soft X-rays to provide valuable insights into understanding intracellular dynamics.</p></div>","PeriodicalId":15726,"journal":{"name":"Journal of Electron Spectroscopy and Related Phenomena","volume":"267 ","pages":"Article 147380"},"PeriodicalIF":1.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43399011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0