Journal of experimental zoology. Part A, Ecological and integrative physiology最新文献

{"title":"Seasonal mRNA Expression of Circadian Clock Genes in the Lizard Brain","authors":"Taylor L. Grossen,&nbsp;Alexus Bunnam,&nbsp;Rachel E. Cohen","doi":"10.1002/jez.2889","DOIUrl":"10.1002/jez.2889","url":null,"abstract":"<div>\u0000 \u0000 <p>Seasonally breeding animals undergo physiological and behavioral changes to time reproduction to occur during specific seasons. These changes are regulated by changing environmental conditions, which may be communicated to the brain using the central circadian clock. This clock consists of a daily oscillation in the expression of several core genes, including <i>period</i> (<i>per</i>), <i>cryptochrome</i> (<i>cry</i>), <i>circadian locomotor output cycles kaput</i> (<i>clock</i>), and <i>basic helix-loop-helix ARNT-like protein 1</i> (<i>bmal1</i>). We began to examine seasonal regulation of four core circadian clock genes in a dissection of the reptile brain containing the hypothalamus—<i>per1, cry1, bmal1 and clock</i>. Our study focused on examining mRNA expression in the morning and compared levels between breeding and nonbreeding animals. We found that <i>per1</i> and <i>bmal1</i> mRNA expression was highest in the nonbreeding compared to breeding season in the anole hypothalamus. We also found that <i>cry1</i> mRNA expression was higher in the female compared to the male anole hypothalamus. We found support for the idea that core circadian genes play a role in regulating changes between the seasons and/or sexes, although more work is needed to elucidate what processes might be differentially regulated. To our knowledge, this is the first examination of the expression of these four genes in the reptilian brain.</p></div>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 2","pages":"278-284"},"PeriodicalIF":1.9,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transition to Piscivory Seen Through Brain Transcriptomics in a Juvenile Percid Fish: Complex Interplay of Differential Gene Transcription, Alternative Splicing, and ncRNA Activity","authors":"Radka Symonová,&nbsp;Tomáš Jůza,&nbsp;Million Tesfaye,&nbsp;Marek Brabec,&nbsp;Daniel Bartoň,&nbsp;Petr Blabolil,&nbsp;Vladislav Draštík,&nbsp;Luboš Kočvara,&nbsp;Milan Muška,&nbsp;Marie Prchalová,&nbsp;Milan Říha,&nbsp;Marek Šmejkal,&nbsp;Allan T. Souza,&nbsp;Zuzana Sajdlová,&nbsp;Michal Tušer,&nbsp;Mojmír Vašek,&nbsp;Cene Skubic,&nbsp;Jakub Brabec,&nbsp;Jan Kubečka","doi":"10.1002/jez.2886","DOIUrl":"10.1002/jez.2886","url":null,"abstract":"<p>Pikeperch (<i>Sander Lucioperca</i>) belongs to main predatory fish species in freshwater bodies throughout Europe playing the key role by reducing planktivorous fish abundance. Two size classes of the young-of-the-year (YOY) pikeperch are known in Europe and North America. Our long-term fish survey elucidates late-summer size distribution of YOY pikeperch in the Lipno Reservoir (Czechia) and recognizes two distinct subcohorts: smaller pelagic planktivores heavily outnumber larger demersal piscivores. To explore molecular mechanisms accompanying the switch from planktivory to piscivory, we compared brain transcriptomes of both subcohorts and identified 148 differentially transcribed genes. The pathway enrichment analyses identified the piscivorous phase to be associated with genes involved in collagen and extracellular matrix generation with numerous Gene Ontology (GO), while the planktivorous phase was associated with genes for non-muscle-myosins (NMM) with less GO terms. Transcripts further upregulated in planktivores from the periphery of the NMM network were <i>Pmchl</i>, <i>Pomcl</i>, and <i>Pyyb</i>, all involved also in appetite control and producing (an)orexigenic neuropeptides. Noncoding RNAs were upregulated in transcriptomes of planktivores including three transcripts of snoRNA U85. Thirty genes mostly functionally unrelated to those differentially transcribed were alternatively spliced between the subcohorts. Our results indicate planktivores as potentially driven by voracity to initiate the switch to piscivory, while piscivores undergo a dynamic brain development. We propose a spatiotemporal spreading of juvenile development over a longer period and larger spatial scales through developmental plasticity as an adaptation to exploiting all types of resources and decreasing the intraspecific competition.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 2","pages":"257-277"},"PeriodicalIF":1.9,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jez.2886","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of Insect Hormones in Different Types of Diapause in Chilo Partellus (Swinhoe)","authors":"Aditya K. Tanwar,&nbsp;Mukesh K. Dhillon,&nbsp;Neha Trivedi,&nbsp;Fazil Hasan,&nbsp;Jagbir S. Kirti","doi":"10.1002/jez.2883","DOIUrl":"10.1002/jez.2883","url":null,"abstract":"<div>\u0000 \u0000 <p>Maize stem borer, <i>Chilo partellus</i> (Swinhoe) is a key pest of maize and sorghum. It undergoes both in estivation and hibernation depending on prevailing environmental conditions. Present investigations were aimed to decipher the regulation of ecdysone, 20-hydroxyecdysone (20E) and juvenile hormone III (JH III) during different stages (prediapause, diapause and post-diapause/pupae) of hibernation and estivation as compared to counterpart nondiapause <i>C. partellus</i>. Significant variations were found in the ecdysone, 20E and JH III titers in the hemolymph of different stages of hibernation and estivation over the nondiapause <i>C. partellus</i>. At the prediapause stages of hibernation and estivation, the 20E was greater than the ecdysone. However, the ecdysone and 20E downregulated, while JH III upregulated during prediapause stages of hibernation and estivation as compared to nondiapause larvae. During diapause, 20E decreased in hibernation, and increased in estivation as compared to their respective prediapause stages. The JH III significantly upregulated in diapause stages of hibernation and estivation over the nondiapause larvae. However, it was significantly greater in prediapause and diapause stages of estivation as compared to hibernation strain. During post-diapause stage, the ecdysone and 20E titers were greater in estivation than in the hibernation and nondiapause strains, while JH III was greater in nondiapause than in the hibernation and estivation strains. These findings suggest the crucial role of these hormones in establishing switch between different stages of diapause and metamorphosis in <i>C. partellus</i>, which could further be useful to strategize sustainable management of <i>C. partellus</i>.</p></div>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 2","pages":"247-256"},"PeriodicalIF":1.9,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and Histological Studies of the Bronchial and Parabronchial System of the White Pekin Duck (Anas platyrhynchos)","authors":"Alaa Abd El Moneam,&nbsp;Ashraf El Sharaby,&nbsp;Asmaa Aboelnour,&nbsp;Mohamed M. A. Abumandour,&nbsp;Ahmed G. Nomir","doi":"10.1002/jez.2884","DOIUrl":"10.1002/jez.2884","url":null,"abstract":"<div>\u0000 \u0000 <p>The present study aimed to provide comprehensive morphological features of the bronchial and parabronchial systems using cast, histological, histochemical, and scanning electron microscopy techniques, with new insights into the parabronchial topographic distribution system on <span>22</span> white Pekin ducks. Casting illustrated that the medioventral secondary bronchi (MVSB) were the largest, but the posterior (POSB) ones were the smallest. The primary (PB) and secondary bronchi (SB) were lined with thin pseudostratified, ciliated columnar epithelium. PB contained discontinuous hyaline cartilage plates interconnected by a membrane of fibrous CT with chondrocytes, while SB had mucous glands. There were two types of hexagonal parabronchi with different lumen shapes: circular in neopulmonic and longitudinal in paleopulmonic. The parabronchi had numerous atria opened into the lumen and guarded by different directed muscles: horizontal in the neopulmonic and vertical or horizontal in the paleopulmonic. The atria were lined with squamous to cuboidal cells, forming the interatrial septum (IAS). A funnel atrial duct connecting the atrium to the infundibulum can be branched. The air capillaries were nearby, at a very short distance from the blood capillaries. Despite their small number, air capillaries, which were found in parabronchi, significantly increased in size and diameter. SEM at the 4th torus level showed a parabronchi distribution with elongated paleopulmonic on the dorsomedial part, hexagonal neopulmonic on the ventrolateral part, and some neopulmonic on the medial part. The parabronchial topography distribution exhibited their unique distribution from the 1st to the 6th torus level.</p></div>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 2","pages":"220-235"},"PeriodicalIF":1.9,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142687051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin Alters Preference to Move Toward Monochromatic Lights in Female Syrian Hamsters: A Behavior Associated With Circadian Rhythm","authors":"Amir Farshad Shadman","doi":"10.1002/jez.2885","DOIUrl":"10.1002/jez.2885","url":null,"abstract":"<div>\u0000 \u0000 <p>Different light colors have different effects on endogenous melatonin. The preference for light colors has been studied in various animal species, except hamsters. Additionally, no research has been done on how melatonin affects color preference. In this study, I investigated whether melatonin can influence Syrian hamsters' preferences for various light colors. Eighteen female Syrian hamsters were divided into a control group and a test group orally administered 0.01 mg melatonin daily for 30 days. On Day 31, I placed each hamster in the test box at four stages: dark mid-phase; beginning, middle, and end of day. The box had four areas with red, yellow, green, and blue lamps. In each stage, the hamsters' movements were recorded for 5 min. I tested the effects of color, stage, and melatonin treatment using a mixed model analysis. The preferences of both groups changed between the stages (<i>p</i> &lt; 0.001) with the except stages 1 and 4 of the control group (<i>p</i> = 0.012); and stages 2 and 3 of the test group for the yellow color (<i>p</i> = 0.104). There was a significant difference between the test and the control groups in all stages and all colors (<i>p</i> &lt; 0.001) except the green light color in stage 2 (<i>p</i> = 0.007). The results suggest that exogenous melatonin controls the preference for monochromatic light by an unknown mechanism. Circadian endogenous melatonin levels are also effective. Scientists must consider melatonin levels in studies evaluating responses to light.</p></div>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 2","pages":"236-246"},"PeriodicalIF":1.9,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142687045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant and Antiapoptotic Effects of Selenium And Nano Selenium-Loaded Exosomes on Hepatic Dysfunction of Type 1 Diabetic Rats","authors":"Layla A. Alahmari,&nbsp;Lashin S. Ali,&nbsp;Hoda A. Fansa,&nbsp;Dalal S. Alshaya,&nbsp;Fawziah A. Al-Salmi,&nbsp;Ehab I. El-Hallous,&nbsp;Mamdouh Eldesoqui,&nbsp;Fahmy Gad Elsaid,&nbsp;Eman Fayad,&nbsp;Ahmed A. El-Mansy,&nbsp;Ghadi Alsharif,&nbsp;Dlovan Y. Khalil,&nbsp;Maryam Bakir Mahmood,&nbsp;Rozhan Yassin Khalil,&nbsp;Hanan M. Rashwan,&nbsp;Shady G. El-Sawah","doi":"10.1002/jez.2881","DOIUrl":"10.1002/jez.2881","url":null,"abstract":"<div>\u0000 \u0000 <p>Mesenchymal stem cells-derived exosomes (MSCs-EXs) applications have brought a key breakthrough in treating type 1 diabetes mellitus (T1DM) and its diabetic complications. However, various recent strategies aimed to construct prominent engineered EXs with greater precision and higher efficiency for diabetes syndrome were conducted. In this research, we seek to enhance the medicinal potentialities of MSCs-EXs on type 1 diabetic rats' hepatic complications, via loading with either selenium (Se) or nano selenium (NSe) particles. For consecutive 4-weeks, rats were divided into 8 groups as; control, EXs, EXs + Se, EXs + NSe, STZ-diabetic (D), D + EXs, D + EXs + Se, and D + EXs + NSe groups. The three diabetic-treated groups manifested a significant reduction in hepatic contents of oxidative stress (OS) (MDA, NO, and H₂O₂) inflammatory (IL-6, TNF-α, and TGF-β), and apoptotic (P53, BAX, caspase-3, and Bcl2) markers, with marked elevation in hepatic antioxidant levels (GSH, GPX, SOD, and CAT). Such results were supported by the marked diminish in serum total proteins, liver function enzymes (AST, ALT, and bilirubin), and both serum and liver lipid profile fractions. In addition, hepatic histological examination showed marked improvement in liver architecture of all treated diabetic rats' groups, compared to diabetic untreated rats. Significantly, diabetic rats with EXs loaded with NSe exhibited the most therapeutic superiority.</p></div>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 2","pages":"211-219"},"PeriodicalIF":1.9,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142621751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Postprandial Sleep in Short-Sleeping Mexican Cavefish","authors":"Kathryn Gallman,&nbsp;Aakriti Rastogi,&nbsp;Owen North,&nbsp;Morgan O'Gorman,&nbsp;Pierce Hutton,&nbsp;Evan Lloyd,&nbsp;Wesley C. Warren,&nbsp;Johanna E. Kowalko,&nbsp;Erik R. Duboue,&nbsp;Nicolas Rohner,&nbsp;Alex C. Keene","doi":"10.1002/jez.2880","DOIUrl":"10.1002/jez.2880","url":null,"abstract":"<p>Interactions between sleep and feeding behaviors are critical for adaptive fitness. Diverse species suppress sleep when food is scarce to increase the time spent foraging. Postprandial sleep, an increase in sleep time following a feeding event, has been documented in vertebrate and invertebrate animals. While interactions between sleep and feeding appear to be highly conserved, the evolution of postprandial sleep in response to changes in food availability remains poorly understood. Multiple populations of the Mexican cavefish, <i>Astyanax mexicanus</i>, have independently evolved sleep loss and increased food consumption compared to surface-dwelling fish of the same species, providing the opportunity to investigate the evolution of interactions between sleep and feeding. Here, we investigate the effects of feeding on sleep in larval and adult surface fish, and in two parallelly evolved cave populations of <i>A. mexicanus</i>. Larval surface and cave populations of <i>A. mexicanus</i> increase sleep immediately following a meal, providing the first evidence of postprandial sleep in a fish model. The amount of sleep was not correlated to meal size and occurred independently of feeding time. In contrast to larvae, postprandial sleep was not detected in adult surface or cavefish, which can survive for months without food. Together, these findings reveal that postprandial sleep is present in multiple short-sleeping populations of cavefish, suggesting sleep-feeding interactions are retained despite the evolution of sleep loss. These findings raise the possibility that postprandial sleep is critical for energy conservation and survival in larvae that are highly sensitive to food deprivation.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 10","pages":"1084-1096"},"PeriodicalIF":1.9,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11579814/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142620576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Older Amphibian Larvae Are More Sensitive to Ultraviolet Radiation and Experience More Sublethal Carryover Effects Post-Metamorphosis","authors":"Niclas U. Lundsgaard,&nbsp;Craig E. Franklin,&nbsp;Rebecca L. Cramp","doi":"10.1002/jez.2882","DOIUrl":"10.1002/jez.2882","url":null,"abstract":"<div>\u0000 \u0000 <p>Elevated ultraviolet radiation (UVR) is postulated as one of multiple, interrelated environmental stressors driving amphibian population declines globally. However, key knowledge gaps remain in elucidating the link between elevated UVR and amphibian declines in a changing climate, including whether timing and irradiance of UVR exposure in early life dictates the onset of detrimental carryover effects post-metamorphosis. In this study, striped marsh frog larvae (<i>Limnodynastes peronii</i>) were exposed to UVR at one of two different irradiances for up to 7 days, either as hatchlings (Gosner stage 23) or as older larvae (Gosner stage 25−28). These animals were then reared to metamorphosis in the absence of UVR to examine independent and interactive carryover effects throughout development. Older larvae were more sensitive to UVR than hatchlings, with 53.1% and 15.6% mortality in larvae exposed to high and low irradiance respectively, compared with no mortality of hatchlings in either irradiance treatment. Irradiance and timing of UVR exposure had interactive effects on larval body length, causing stunted growth patterns and a lack of compensatory growth following UVR exposure, particularly in animals exposed to high irradiance UVR later in development. Timing of UVR exposure also determined the severity of carryover effects into metamorphosis, including delayed metamorphosis and the first published account (to our knowledge) of latent UVR-induced depigmentation in an amphibian. These findings highlight how acute changes to the larval UVR exposure regime can impact on amphibian health later in life, with implications for our understanding of the effects of climate change on UVR-related amphibian declines.</p></div>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 2","pages":"197-210"},"PeriodicalIF":1.9,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142620312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold Tolerance and Physiological Response of Natural Overwintering Pomacea canaliculata in South China","authors":"Zhong Qin,&nbsp;Zeheng Xiao,&nbsp;Chuang Li,&nbsp;Jimin Liu,&nbsp;Fucheng Yao,&nbsp;Xiaoting Lin,&nbsp;Jiaen Zhang,&nbsp;Yiman Liu","doi":"10.1002/jez.2874","DOIUrl":"10.1002/jez.2874","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Pomacea canaliculata</i> (Lamarck 1822), a freshwater gastropod indigenous to lower Del Plata Basin of Argentina, has become the most destructive and invasive rice pests in south China since its introduction in the 1980s. In Guangdong, the main production areas for double rice, most of <i>P. canaliculata</i> overwinter in paddy field ditches after late-rice harvesting in mid-November and diapause to temporarily to avoid the damaging effects of extreme low temperatures. This pest aroused from diapause and migrated to the paddy field after early-rice reviving in next late March. Overwintering and cold tolerance of natural <i>P. canaliculata</i> have a non-negligible impact on population dynamics and distribution in the following year. We tested the supercooling capability, levels of cryoprotectant synthesis, activity of antioxidant defense system (antioxidant enzymes and reduced glutathione), and degree of oxidative damage (concentration of malondialdehyde as an index of lipid peroxidation) monthly, using natural <i>P. canaliculata</i> samples with a size-gender structure (i.e., juveniles, female, and male adults) from experimental ponds during the period of mid-November to the following April. <i>P. canaliculata</i> survived the winter with a monthly death rate of 7%–16.5% in coldest January. The supercooling point (SCP) of overwintering <i>P. canaliculata</i> decreased initially before increasing subsequently with monthly changes in water temperature. <i>P. canaliculata</i> accumulated a high glycogen content before December, which depleted towards the end of January, while lipid content reached peak in January and depleted since February. Activity of antioxidant defense system of <i>P. canaliculata</i> exhibited significant monthly differences and showed relatively higher size heterogeneity than monthly variations. The results contribute to the knowledge of adaptability in overwintering <i>P. canaliculata</i> and help to understand the mechanism of the invasive success of this species.</p></div>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 2","pages":"172-183"},"PeriodicalIF":1.9,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142583317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mediterranean Mussels (Mytilus galloprovincialis) Under Salinity Stress: Effects on Antioxidant Capacity and Gill Structure","authors":"Aleksandra Andreyeva,&nbsp;Olga Gostyukhina,&nbsp;Tatyana Gavruseva,&nbsp;Tatyana Sigacheva,&nbsp;Anastasiya Tkachuk,&nbsp;Mariya Podolskaya,&nbsp;Elina Chelebieva,&nbsp;Ekaterina Kladchenko","doi":"10.1002/jez.2879","DOIUrl":"10.1002/jez.2879","url":null,"abstract":"<div>\u0000 \u0000 <p>Bivalve mollusks frequently experience salinity fluctuations that may drive oxidative stress (OS) in the organism. Here we investigated OS markers and histopathological changes in gills and hemolymph of Mediterranean mussels <i>Mytilus galloprovincialis</i> Lamarck, 1819 exposed to a wide range of salinities (6, 10, 14, 24, and 30 ppt). Mussels were captured at the shellfish farm with the salinity 18 ppt and then exposed to hypo- and hypersaline conditions in the laboratory. Indicators of redox balance in hemocytes (intracellular reactive oxygen species (ROS) levels, DNA damage) and gills (thiobarbituric acid reactive substances (TBARS), protein carbonyls (PC), activity of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) were measured. The effect of salinity stress on microstructure of gills has been evaluated as well. The results revealed induction of OS in tissues and cells of mussels for both experimental increase and decrease salinity modelings. Hemocytes showed higher sensitivity to osmotic stress compared to gills. In gills TBARS were stable in all experimental groups and PC increased only at salinity 6 ppt. The activity of SOD, CAT and GPx in gills decreased only in mussels acclimated to salinity 24 ppt and further salinisation up to 30 ppt was associated with the recovery of the activity of all enzymes. Major histopathological changes in gills upon salinity fluctuations included inflammatory reactions, circulatory alterations, regressive and progressive changes. Our findings clearly indicate that salinity fluctuations promote OS at cellular and tissue level and also affect microstructure of gills in mussels. The results provide new insights into the mechanisms of osmotic stress in bivalves.</p></div>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 2","pages":"184-196"},"PeriodicalIF":1.9,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142583321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}