盐度胁迫下的地中海贻贝(Mytilus galloprovincialis):对抗氧化能力和鳃结构的影响

IF 1.9 3区 生物学 Q1 ZOOLOGY
Aleksandra Andreyeva, Olga Gostyukhina, Tatyana Gavruseva, Tatyana Sigacheva, Anastasiya Tkachuk, Mariya Podolskaya, Elina Chelebieva, Ekaterina Kladchenko
{"title":"盐度胁迫下的地中海贻贝(Mytilus galloprovincialis):对抗氧化能力和鳃结构的影响","authors":"Aleksandra Andreyeva, Olga Gostyukhina, Tatyana Gavruseva, Tatyana Sigacheva, Anastasiya Tkachuk, Mariya Podolskaya, Elina Chelebieva, Ekaterina Kladchenko","doi":"10.1002/jez.2879","DOIUrl":null,"url":null,"abstract":"<p><p>Bivalve mollusks frequently experience salinity fluctuations that may drive oxidative stress (OS) in the organism. Here we investigated OS markers and histopathological changes in gills and hemolymph of Mediterranean mussels Mytilus galloprovincialis Lamarck, 1819 exposed to a wide range of salinities (6, 10, 14, 24, and 30 ppt). Mussels were captured at the shellfish farm with the salinity 18 ppt and then exposed to hypo- and hypersaline conditions in the laboratory. Indicators of redox balance in hemocytes (intracellular reactive oxygen species (ROS) levels, DNA damage) and gills (thiobarbituric acid reactive substances (TBARS), protein carbonyls (PC), activity of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) were measured. The effect of salinity stress on microstructure of gills has been evaluated as well. The results revealed induction of OS in tissues and cells of mussels for both experimental increase and decrease salinity modelings. Hemocytes showed higher sensitivity to osmotic stress compared to gills. In gills TBARS were stable in all experimental groups and PC increased only at salinity 6 ppt. The activity of SOD, CAT and GPx in gills decreased only in mussels acclimated to salinity 24 ppt and further salinisation up to 30 ppt was associated with the recovery of the activity of all enzymes. Major histopathological changes in gills upon salinity fluctuations included inflammatory reactions, circulatory alterations, regressive and progressive changes. Our findings clearly indicate that salinity fluctuations promote OS at cellular and tissue level and also affect microstructure of gills in mussels. The results provide new insights into the mechanisms of osmotic stress in bivalves.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Mediterranean Mussels (Mytilus galloprovincialis) Under Salinity Stress: Effects on Antioxidant Capacity and Gill Structure.\",\"authors\":\"Aleksandra Andreyeva, Olga Gostyukhina, Tatyana Gavruseva, Tatyana Sigacheva, Anastasiya Tkachuk, Mariya Podolskaya, Elina Chelebieva, Ekaterina Kladchenko\",\"doi\":\"10.1002/jez.2879\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Bivalve mollusks frequently experience salinity fluctuations that may drive oxidative stress (OS) in the organism. Here we investigated OS markers and histopathological changes in gills and hemolymph of Mediterranean mussels Mytilus galloprovincialis Lamarck, 1819 exposed to a wide range of salinities (6, 10, 14, 24, and 30 ppt). Mussels were captured at the shellfish farm with the salinity 18 ppt and then exposed to hypo- and hypersaline conditions in the laboratory. Indicators of redox balance in hemocytes (intracellular reactive oxygen species (ROS) levels, DNA damage) and gills (thiobarbituric acid reactive substances (TBARS), protein carbonyls (PC), activity of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) were measured. The effect of salinity stress on microstructure of gills has been evaluated as well. The results revealed induction of OS in tissues and cells of mussels for both experimental increase and decrease salinity modelings. Hemocytes showed higher sensitivity to osmotic stress compared to gills. In gills TBARS were stable in all experimental groups and PC increased only at salinity 6 ppt. The activity of SOD, CAT and GPx in gills decreased only in mussels acclimated to salinity 24 ppt and further salinisation up to 30 ppt was associated with the recovery of the activity of all enzymes. Major histopathological changes in gills upon salinity fluctuations included inflammatory reactions, circulatory alterations, regressive and progressive changes. Our findings clearly indicate that salinity fluctuations promote OS at cellular and tissue level and also affect microstructure of gills in mussels. The results provide new insights into the mechanisms of osmotic stress in bivalves.</p>\",\"PeriodicalId\":15711,\"journal\":{\"name\":\"Journal of experimental zoology. Part A, Ecological and integrative physiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2024-11-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of experimental zoology. Part A, Ecological and integrative physiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1002/jez.2879\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ZOOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of experimental zoology. Part A, Ecological and integrative physiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1002/jez.2879","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ZOOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

双壳软体动物经常经历盐度波动,这可能会导致生物体内的氧化应激(OS)。在此,我们研究了暴露于各种盐度(6、10、14、24 和 30 ppt)下的地中海贻贝(Mytilus galloprovincialis Lamarck, 1819)鳃和血淋巴中的氧化应激标记和组织病理学变化。在贝类养殖场捕获的贻贝盐度为 18 ppt,然后在实验室中暴露于低盐和高盐条件下。测量了血细胞(细胞内活性氧(ROS)水平、DNA 损伤)和鳃(硫代巴比妥酸活性物质(TBARS)、蛋白质羰基(PC)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GPx)的活性)中的氧化还原平衡指标。还评估了盐度胁迫对鳃微观结构的影响。结果显示,在盐度升高和降低的实验模型中,贻贝的组织和细胞都会诱发OS。与鳃相比,血球对渗透压的敏感性更高。在所有实验组中,鳃中的 TBARS 都保持稳定,只有在盐度为 6 ppt 时 PC 才会增加。只有在盐度为 24 ppt 时,贻贝鳃中的 SOD、CAT 和 GPx 活性才会下降,盐度进一步升高到 30 ppt 时,所有酶的活性才会恢复。盐度波动时贻贝鳃的主要组织病理学变化包括炎症反应、循环系统改变、退行性和进行性变化。我们的研究结果清楚地表明,盐度波动会在细胞和组织水平上促进OS,也会影响贻贝鳃的微观结构。这些结果为研究双壳贝类的渗透压力机制提供了新的视角。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mediterranean Mussels (Mytilus galloprovincialis) Under Salinity Stress: Effects on Antioxidant Capacity and Gill Structure.

Bivalve mollusks frequently experience salinity fluctuations that may drive oxidative stress (OS) in the organism. Here we investigated OS markers and histopathological changes in gills and hemolymph of Mediterranean mussels Mytilus galloprovincialis Lamarck, 1819 exposed to a wide range of salinities (6, 10, 14, 24, and 30 ppt). Mussels were captured at the shellfish farm with the salinity 18 ppt and then exposed to hypo- and hypersaline conditions in the laboratory. Indicators of redox balance in hemocytes (intracellular reactive oxygen species (ROS) levels, DNA damage) and gills (thiobarbituric acid reactive substances (TBARS), protein carbonyls (PC), activity of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) were measured. The effect of salinity stress on microstructure of gills has been evaluated as well. The results revealed induction of OS in tissues and cells of mussels for both experimental increase and decrease salinity modelings. Hemocytes showed higher sensitivity to osmotic stress compared to gills. In gills TBARS were stable in all experimental groups and PC increased only at salinity 6 ppt. The activity of SOD, CAT and GPx in gills decreased only in mussels acclimated to salinity 24 ppt and further salinisation up to 30 ppt was associated with the recovery of the activity of all enzymes. Major histopathological changes in gills upon salinity fluctuations included inflammatory reactions, circulatory alterations, regressive and progressive changes. Our findings clearly indicate that salinity fluctuations promote OS at cellular and tissue level and also affect microstructure of gills in mussels. The results provide new insights into the mechanisms of osmotic stress in bivalves.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of experimental zoology. Part A, Ecological and integrative physiology
Journal of experimental zoology. Part A, Ecological and integrative physiology Biochemistry, Genetics and Molecular Biology-Molecular Biology
CiteScore
4.90
自引率
3.60%
发文量
0
期刊介绍: The Journal of Experimental Zoology – A publishes articles at the interface between Development, Physiology, Ecology and Evolution. Contributions that help to reveal how molecular, functional and ecological variation relate to one another are particularly welcome. The Journal publishes original research in the form of rapid communications or regular research articles, as well as perspectives and reviews on topics pertaining to the scope of the Journal. Acceptable articles are limited to studies on animals.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信