Journal of experimental zoology. Part A, Ecological and integrative physiology最新文献

{"title":"Muscle and Metabolic Genes Are Differentially Expressed During Thermal Acclimation by the Brook Trout Myotome","authors":"David J. Coughlin,&nbsp;Madeline D. Dutterer,&nbsp;Zachary D. LaMonica,&nbsp;Evelyn M. Peyton,&nbsp;Elizabeth S. Kwon,&nbsp;Kathleen A. Hittle","doi":"10.1002/jez.2901","DOIUrl":"10.1002/jez.2901","url":null,"abstract":"<div>\u0000 \u0000 <p>Cold-water fishes, such as Brook trout (<i>Salvelinus fontinalis</i>), are being challenged by the consequences of climate change. The ability of these fish to acclimate to warmer environmental conditions is vital to their survival. Acclimation to warmer water may allow brook trout to reduce the metabolic costs of higher temperatures. Previous work has shown that brook trout display a significant thermal acclimation response in their myotomal muscle, with slower contractile properties observed in warm acclimated fish. In this study, gene expression was examined in hatchery brook trout acclimated to a range in temperatures (4, 10 or 20°C). Brook trout displayed variations in gene expression in their myotomal muscle in accordance with acclimation temperature. Genes important for muscle function, cellular metabolism, protein degradation, and stress response showed variation to both warm (20°C) and cold (4°C) acclimation. The warm acclimated fish also showed decreased expression of genes associated with aerobic metabolism and increased expression of genes for heat shock proteins, while the cold acclimated fish showed increased expression of genes associated with lipid metabolism and protein turnover. α-tubulin displayed a close association with thermal acclimation, increasing in expression with acclimation temperature. The patterns of muscle gene expression were the opposite of what was expected. Although warm acclimated fish have previously been shown to display slow muscle contractile properties, this study found that warm acclimation is associated with increased expression of genes for kinetically faster isoforms of important muscle proteins. Collectively, the results demonstrate a robust response to elevated temperature in the hatchery fish greater than 10,000 genes showing differential expression with temperature. These results provide a roadmap for the analysis of the acclimation response of native populations of brook trout encountering climate change.</p></div>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 3","pages":"416-426"},"PeriodicalIF":1.9,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Body Condition and Blood Biochemistry of Free-Range Caiman latirostris in Northeast Brazilian Atlantic Forest","authors":"Gabriela Mota Gama,&nbsp;Luiza Figueiredo Passos,&nbsp;Adriano Pereira Paglia,&nbsp;Marcos Eduardo Coutinho","doi":"10.1002/jez.2897","DOIUrl":"10.1002/jez.2897","url":null,"abstract":"<div>\u0000 \u0000 <p>The Atlantic Forest broad-snouted caiman (<i>Caiman latirostris</i>) inhabits regions within one of the world's most ecologically diverse ecosystems, yet few studies have explored the relationship between body condition, blood biochemistry, and environmental factors in the wild. Our study investigated the effects of sex, ontogeny, habitat, and environmental variables on the body condition and blood biochemistry of free-ranging caimans from the state of Alagoas, Northeast Brazil. From 2020 to 2022, we captured 75 caimans across three sites in different seasons. Results revealed sex-specific responses to seasonal and Interannual weather changes, with females showing higher body condition in the wet season, while males peaked in the dry season. Elevated glucose, total protein, albumin, triglycerides, and fructosamine were linked to higher body condition and larger individuals, while elevated aspartate aminotransferase to low body condition. Seasonal rainfall influenced blood parameters, with the dry season associated with higher creatinine, calcium, and alanine aminotransferase levels, and the wet season with higher total protein, sodium, and potassium. Differences in glucose, alkaline phosphatase, and gamma-glutamyl transferase across sites pointed to physiological effects of human activities. Blood biochemical values varied widely, with some exceeding reported species ranges. These findings highlight the need to interpret physiological data within the context of local habitat and environmental conditions. Conservation strategies should go beyond species presence and habitat preservation, incorporating pollution control. Our study advances understanding of <i>Caiman latirostris</i> ecophysiology, offering valuable insights for the conservation and management of crocodilian populations in both wild and captive environments.</p></div>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 3","pages":"383-399"},"PeriodicalIF":1.9,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142970997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant and Anti-Inflammatory Impacts of Soft Tissue Crude Extract and Mucous of Snail Helix aspersa on an Excision Wound Model in Mice","authors":"Hoda H. Abdel-Azeem,&nbsp;Gamalat Y. Osman,&nbsp;Dalia S. Morsi","doi":"10.1002/jez.2895","DOIUrl":"10.1002/jez.2895","url":null,"abstract":"<div>\u0000 \u0000 <p>Wound healing is a complex natural process in which tissue requires recovering injured tissue cells. <i>Helix aspersa</i> has a high nutritional value and is considered a rich natural source of antioxidants and anti-inflammatory agents. So, this study aimed to assess the effect of soft tissue crude extract and mucous of <i>H. aspersa</i> topically applied as a gel for 12 days. The wounds were observed and photographed twice a week. The inflammatory, oxidative stress markers and matrix metalloproteinases were evaluated in skin tissue homogenate and CD3⁺ and CD69⁺ T lymphocytes were detected in wound tissue. Data showed that a comparison of applying soft tissue crude extract and mucous of <i>H. aspersa</i> to skin wounds enhanced the healing process, resulting in a significant decrease in dermal inflammation compared to untreated mice. Also, they significantly increased the antioxidant enzyme activities with reduced malondialdehyde (MDA) levels in wound tissues. The levels of matrix metalloproteases-2 and -9 were significantly decreased and the immune status was enhanced in the wound environment by increasing proportions of CD3⁺ and CD69⁺ T lymphocytes. <i>H. aspersa</i> mucous and soft tissue crude extract are viable substitutes for synthetic topical wound therapies with anti-inflammatory, antioxidant, and immunomodulatory potencies, with a preference for the crude soft tissue extract based on the outcomes.</p></div>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 3","pages":"373-382"},"PeriodicalIF":1.9,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142970991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of Branchial Anoctamin 1 Expression in Freshwater- and Seawater-Acclimated Japanese Medaka, Oryzias latipes","authors":"Norifumi Konno,&nbsp;Ayane Togashi,&nbsp;Hiroshi Miyanishi,&nbsp;Morio Azuma,&nbsp;Tomoya Nakamachi,&nbsp;Kouhei Matsuda","doi":"10.1002/jez.2894","DOIUrl":"10.1002/jez.2894","url":null,"abstract":"<div>\u0000 \u0000 <p>In euryhaline teleosts, the cystic fibrosis transmembrane conductance regulator (CFTR) in seawater (SW)-type chloride cells facilitates apical Cl⁻ secretion for SW adaptation, while alternative Cl⁻ excretion pathways remain understudied. This study investigates the role of the calcium-activated chloride channel, Anoctamin 1 (ANO1), in the gills of the euryhaline Japanese medaka (<i>Oryzias latipes</i>) under hyperosmolality and cortisol (CORT) influence. Acclimation to artificial SW, NaCl, mannitol, or glucose significantly upregulated <i>ANO1</i> and <i>CFTR</i> mRNA expression in gills, unlike urea treatment. In situ hybridization revealed <i>ANO1</i> mRNA in chloride cells co-expressing <i>CFTR</i> and <i>Na</i>⁺, <i>K</i>⁺-ATPase under hyperosmotic conditions. ANO1 inhibition elevated plasma Cl⁻ concentration, indicating impaired Cl⁻ excretion. CORT or dexamethasone administration in freshwater (FW) fish significantly increased branchial <i>ANO1</i> and <i>CFTR</i> mRNA expression, an effect attenuated by the glucocorticoid receptor (GR) antagonist RU486. Hyperosmotic treatment of isolated gill tissues rapidly induced <i>ANO1</i> mRNA expression independent of <i>CFTR</i> mRNA changes, and this induction was unaffected by RU486. These findings highlight the dual regulation of ANO1 expression via hyperosmolality-induced cellular response and the CORT–GR system. Thus, branchial ANO1 may likely complement CFTR in Cl⁻ excretion, playing a key role in the hyperosmotic adaptation of euryhaline teleosts.</p></div>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 3","pages":"356-372"},"PeriodicalIF":1.9,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Nematocidal Effect of Leiurus quinquestratus Scorpion Venom on Toxocara canis in Mice Model","authors":"Wesam M. Salama,&nbsp;Rasha A. Elmahy","doi":"10.1002/jez.2893","DOIUrl":"10.1002/jez.2893","url":null,"abstract":"<div>\u0000 \u0000 <p>One neglected zoonotic illness is toxocariasis. There are not enough anthelmintic drugs in the market to treat low-effectiveness toxocariasis against migrating larvae. Therefore, it is critical to find new, safe alternatives to toxocariasis treatment today. The venom of the <i>Leiurus quinquestratus</i> scorpion (LQV) has numerous medicinal uses. The purpose of this study was to conduct that LQV had an effect on <i>T. canis</i> larvae in the model of mice. Fifty male mice were divided into five groups (<i>n</i> = 10). Group 1 (Gp1) was the negative group, and from Gp2-Gp5 had been infected with 800–1000 <i>T. canis</i> embryonated eggs. Gp2 was left as positive control, Gp3 was orally administrated with albendazole (Alb) (100 mg/kg/b.wt) for 7 consecutive days at first day post infection (pi), Gp4 was injected intra-peritoneal (i.p) with LQV (0.03 mg/kg/b.wt) for 15 consecutive days at the first day pi, and Gp5 was given Alb/LQV in the same way and doses as Gp3 and Gp4. At the end of the experiment, at 30th pi, the blood samples were collected for evaluating the biochemical and hematological parameters. The larval count reduction in brain were evaluated, and the histopathological changes in brain and liver were detected. The results showed that, treatment with LQV or Alb/LQV combinations significantly reduced the larval burden in the brain of the infected mice with reduction percentage reached to 70, and 72%, respectively. Interestingly, LQV and Alb/LQV significantly reduced IL-4, IL-10, and TNF –α levels and increased IFN-γ levels. Also, improved the liver transaminases activity, and ameliorated liver and brain architecture. Collectively, LQV may be a promising treatment for <i>T. canis</i> infection and albendazole alternative.</p></div>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 3","pages":"343-355"},"PeriodicalIF":1.9,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond Hunger: A Connection Between Diet and Gonadal Development of a Ladybird Beetle","authors":"Sanjeev Kumar,&nbsp;Deeksha Jattan,&nbsp;Tripti Yadav,&nbsp;Geetanjali Mishra,&nbsp; Omkar","doi":"10.1002/jez.2888","DOIUrl":"10.1002/jez.2888","url":null,"abstract":"<div>\u0000 \u0000 <p>Food availability shapes morphology, physiology, growth, reproduction, and overall fitness of insects. Countless research in coccinellids reported the effect of diet in terms of quality and quantity on reproductive output. But fewer studies have reported the direct effects of food on the gonadal development in both sexes. In nature, ladybird beetle <i>Cheilomenes sexmaculata</i> exhibit food preference, favouring <i>Aphis craccivora</i> (nutritious prey) over <i>Aphis nerii</i> (poor prey). We hypothesized that beetles consuming nutritious prey in abundant quantity will have larger and heavier gonads. The current study assessed the effect of food preferred and its fluctuations on the gonadal development of <i>C. sexmaculata</i>. First instars were collected randomly from experimental stock and reared on two different diet regimes for a lifetime. After eclosion, beetles from each dietary regime were dissected daily to assess gonadal development until maturity. Here, we documented total developmental duration, body weight, weight of gonads, GSI, total area of gonads, length, and number of testicular lobules/ovarioles across different treatments. Both quality and quantity of diet significantly affected development period, body weight, and gonadal parameters in both sexes except for GSI in males and number of ovarioles in females that reared on varied diet quality, which were found to be similar. <i>Aphis craccivora</i> in abundant supply positively affected the growth and development of the gonads in <i>C. sexmaculata</i>. Beetles reared on the abundant supply of nutritious prey with larger and heavier gonads than those reared on limited supply or poor prey. This study improves our understanding of the development of gonads in ladybird beetles, which may be helpful in improving the mass rearing of ladybird beetles.</p></div>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 3","pages":"332-342"},"PeriodicalIF":1.9,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142854176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Day Late, Dollar Short: Runts of Asynchronously Hatched Songbird Broods Have Reduced Survival, Body Size, and Persistent Energy Deficits","authors":"Keegan R. Stansberry,&nbsp;Tosha R. Kelly,&nbsp;Kaitlin E. Couvillion,&nbsp;Allison L. Cannon,&nbsp;Melanie G. Kimball,&nbsp;Hallie B. Callegan,&nbsp;Kevin J. Krajcir,&nbsp;Jeffrey D. Kittilson,&nbsp;Britt J. Heidinger,&nbsp;Christine R. Lattin","doi":"10.1002/jez.2892","DOIUrl":"10.1002/jez.2892","url":null,"abstract":"<div>\u0000 \u0000 <p>Many songbirds begin active incubation after laying their penultimate egg, resulting in synchronous hatching of the clutch except for a last-hatched individual (“runt”) that hatches with a size deficit and competitive disadvantage to siblings when begging for food. However, climate change may elevate temperatures and cause environmental incubation as eggs are laid, resulting in asynchronous hatching and larger size hierarchies among siblings. Although previous work demonstrated that asynchronous hatching reduces nestling growth and survival relative to synchrony, the physiological mechanisms underlying these effects are unclear. To test the effects of asynchronous hatching on runt growth, survival, physiology, and compensatory growth-related tradeoffs, we manipulated incubation temperature in nest boxes of European starlings (<i>Sturnus vulgaris</i>) to increase asynchronous hatching and collected nestling morphological measurements and blood samples to assess physiology and development. Independent of heating treatment, runts from asynchronously hatched nests had lower survival than runts from more synchronous nests. Surviving runts from asynchronous nests were smaller and had reduced stress-induced corticosterone concentrations and reduced circulating glucose compared with runts from synchronous nests. Despite persistent size and energy deficits, runts from asynchronous nests did not have significant deficits in immunity or telomere length when compared with runts from synchronous nests, suggesting no trade-off between investment in immune development or telomere maintenance with growth. Overall, these results suggest that increased asynchrony due to climate change could reduce clutch survival for altricial songbirds, especially for the smallest chicks in a clutch, and that the negative effects of asynchrony may be driven by persistent energetic deficits.</p></div>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 3","pages":"319-331"},"PeriodicalIF":1.9,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142846671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological Effects of Salinity on the Osmotic Properties and Oxidative Stress Responses of the Razor Clam Solen regularis in Don Hoi Lot, Thailand","authors":"Sappasith Dechkittithum,&nbsp;Kannapoj Techawongstien,&nbsp;Juthamas Phothakwanpracha,&nbsp;Phurich Boonsanit,&nbsp;Supanut Pairohakul","doi":"10.1002/jez.2887","DOIUrl":"10.1002/jez.2887","url":null,"abstract":"<div>\u0000 \u0000 <p>Estuaries are diverse coastal ecosystems that act as transitional zones between freshwater and seawater. The Don Hoi Lot tidal flat, located in the upper Gulf of Thailand, is one of Thailand's most important estuarine ecosystems. Nonetheless, the Don Hoi Lot area faces increasing environmental pressures due to human activities and natural changes. One of the most prominent species well-known in this area is the razor clam <i>Solen regularis</i>. This study investigated the effects of salinity fluctuations on the osmotic properties and antioxidant enzyme activities of the five tissues: foot, mantle, adductor muscle, gill, and digestive gland from the razor clam <i>S. regularis</i> collected from the Don Hoi Lot tidal flat. Razor clams were exposed to a range of salinity levels (0–35 PSU) for 7 days. The results indicated that the hemolymph osmolality of <i>S. regularis</i> increased with increasing salinity, demonstrating an osmoconforming pattern. Salinity changes significantly affected the activities of antioxidant enzymes (SOD, CAT, and GPX) in various tissues. In most tissues, SOD and CAT activities increased at higher salinities (30–35 PSU), while GPX activity generally increased across all tissues with increasing salinity. GST activity was not significantly affected by salinity changes. These findings suggest that <i>S. regularis</i> can activate antioxidant defense systems to reduce oxidative stress caused by salinity fluctuations. This study provides valuable insights into the physiological responses of <i>S. regularis</i> to environmental salinity changes, which can inform conservation efforts for this ecologically important species in estuarine ecosystems.</p></div>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 2","pages":"302-313"},"PeriodicalIF":1.9,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142846676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gehyra Geckos Prioritize Warm Over Humid Environments","authors":"Kade Skelton,&nbsp;Kimberley Day,&nbsp;Chava L. Weitzman,&nbsp;Christine Schlesinger,&nbsp;Craig Moritz,&nbsp;Keith Christian","doi":"10.1002/jez.2890","DOIUrl":"10.1002/jez.2890","url":null,"abstract":"<p>Maintaining stable hydric and thermal states are dual challenges for reptiles that inhabit terrestrial environments with variable conditions across time and space. Under some conditions, reptiles face a conundrum where both physiological parameters cannot be simultaneously maintained at preferred levels by behavioral or physiological means. Prioritization of behavioral regulation of hydric or thermal state, and at which point this prioritization changes, was tested for nine species of congeneric tropical geckos by assessing their use of microhabitats with distinct thermal and hydric conditions in a controlled environment. <i>Gehyra</i> geckos were presented with two crevices of contrasting humidity levels, and time spent in either crevice was recorded across three ambient temperature treatments of 32°C, 27°C, and 22°C. Temperatures in the humid crevice matched ambient air temperature, whereas temperature in the dry crevice was maintained at 32°C. In these trials, all species showed greater use of the dry (and warm) crevice in the 27°C and 22°C treatments, while there was no strong preference for the humid or dry crevice in the 32°C treatment. Thus, <i>Gehyra</i> geckos prioritized thermoregulation and maintained thermal state through behavioral responses, and humid microhabitats were not selected even when it did not compromise the animal's thermal state. Although selection for preferred thermal conditions was prioritized in the short term, this does not preclude the possibility that hydric state can be regulated on a seasonal time scale.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 2","pages":"294-301"},"PeriodicalIF":1.9,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jez.2890","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142836928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Curious Case of Eulimnogammarus cyaneus (Dybowsky, 1874): Reproductive Biology of a Widespread Endemic Littoral Amphipod From Lake Baikal","authors":"Polina Drozdova,&nbsp;Zhanna Shatilina,&nbsp;Andrei Mutin,&nbsp;Alexandra Saranchina,&nbsp;Anton Gurkov,&nbsp;Maxim Timofeyev","doi":"10.1002/jez.2891","DOIUrl":"10.1002/jez.2891","url":null,"abstract":"<div>\u0000 \u0000 <p>Comparative studies of reproductive biology and formation of reproductive isolation need appropriate model systems, such as groups of related species. The amphipods (Crustacea: Amphipoda) of ancient Lake Baikal are an attractive group for such works, as they consist of several hundred species that radiated within the lake and have very different levels of intraspecific genetic diversity and reproduction timing. We have previously shown that one of the most widely distributed and best studied littoral species, <i>Eulimnogammarus verrucosus</i> (Gersfeldt, 1858), comprises cryptic species exhibiting a post-zygotic reproductive barrier. The object of this study was <i>Eulimnogammarus cyaneus</i> (Dybowsky, 1874), another widespread endemic littoral Baikal species, which has a surprisingly low genetic diversity within its large geographic range. The aim of this study was to check if the populations isolated by the Angara River source, which is approximately 120-thousand years old, are reproductively compatible. As neither prezygotic nor postzygotic barriers were found, at the moment these populations should be treated as belonging to a single species. At the same time, we found some noteworthy features of reproduction of <i>E. cyaneus</i>. They include successive reproductive cycles with amplexuses formed by females with juveniles in the brood pouch and deposition of unfertilized eggs by females. The former might mean that this species is a relatively promising object for a laboratory culture of Baikal amphipods. Taken together, these results contribute to the establishment of a research framework to look for the correlation between genetic divergence and biological species delimitation.</p></div>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 2","pages":"285-293"},"PeriodicalIF":1.9,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142818244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}