Journal of experimental zoology. Part A, Ecological and integrative physiology最新文献

{"title":"Macro- and micro-morphological comparison of the detailed structure of the oral cavity roof in two different feeding habits marine fishes: Pagrus pagrus and Boops boops","authors":"Mohamed A. M. Alsafy,&nbsp;Manal A. Seif,&nbsp;Ahmed A. El-Mansi,&nbsp;Mohamed El-Beskawy,&nbsp;Mohamed El Dakroury,&nbsp;Mamdouh B. Eldesoqui,&nbsp;Samar M. Ez Elarab,&nbsp;Samir A. A. El-Gendy","doi":"10.1002/jez.2822","DOIUrl":"10.1002/jez.2822","url":null,"abstract":"<p>The feeding habits and habitats of fish influence the morphology of the oral cavity. This study used gross anatomy, light microscopy, and scanning electron microscopy, in addition to morphometric analysis, to investigate the anatomical characteristics of the oral cavity roof in <i>Pagrus pagrus</i> and <i>Boops boops</i>, which have different dietary habits. The oral cavity roof appeared U-shaped and divided into the palate and upper pharyngeal regions. The upper lip of <i>P. pagrus</i> was broad, while <i>B. boops'</i> upper lip was small and thin. Both species had a stratified squamous epithelium with an irregular shape and a folded surface. <i>P. pagrus</i> had a horseshoe-shaped upper velum with a high middle part, and its surface resembled sea waves with obvious mucous-secreting openings with cilia and many folds and grooves between them. <i>B. boops's</i> upper velum was thin and appeared as a triangle pouch with a pointed cranial apex. The palate in both species was narrow in the front and increased in width backward until it ended. The upper pharyngeal teeth in <i>P. pagrus</i> appeared as two patches, separated by a median longitudinal ridge and an anterior V-shape separator. Meanwhile, in <i>B. boops</i>, they appeared as a ball patch on both sides and a separator ridge in the middle. Because <i>P. pagrus</i> fed on harder structures than <i>B. boops</i>, their feeding habits were reflected in the structure of the oral cavity roof. <i>P. pagrus</i>, a carnivorous species, had several rows of sharp upper jaw and upper pharyngeal teeth, thick spinous tubercles on oblique transverse ridges, and massive mucous glands. On the other hand, <i>B. boops</i>, an omnivorous species, had only one row of upper jaw teeth, a few upper pharyngeal teeth scattered on two oval patches, and thin filaments on the oblique transverse ridges.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 7","pages":"783-797"},"PeriodicalIF":1.9,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140665838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimentally elevated corticosterone increases song output and complexity in common mynas","authors":"Juliane Gaviraghi Mussoi,&nbsp;Rebecca A. MacQueen,&nbsp;Margaret C. Stanley,&nbsp;Kristal E. Cain","doi":"10.1002/jez.2817","DOIUrl":"10.1002/jez.2817","url":null,"abstract":"<p>Vocalization is an important communication tool that can reflect many aspects of an individual's internal and external condition. This is especially true for birds. Previous research has shown that bird calls and songs change in response to a variety of potential stressors, although the extent and direction of the changes depend on the nature of the stressor and the environment. Circulating glucocorticoids, such as corticosterone, often increase in response to stressors and mediate some of the observed changes via alterations of the individual's physiological state. Acute elevations of corticosterone often occur as a physiological response to short-term stressors; however, the effects of this elevation on adult vocalizations have not been well documented. Here, we experimentally elevated corticosterone at two different levels using a noninvasive method and examined the effects on the vocal communication of male and female adult common mynas (<i>Acridotheres tristis</i>). Corticosterone elevation temporarily increased song output and some measures of song complexity, while call output decreased. These effects were dosage dependent (higher corticosterone levels had a stronger effect), most evident 40 min after ingestion, and some vocal changes were sex-specific. Future studies should investigate whether the changes in vocal performance due to elevated glucocorticoids have consequences for the birds' behavior, reproductive success, and survival.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 7","pages":"733-742"},"PeriodicalIF":1.9,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jez.2817","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140666625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal methylosome protein 50 is essential for embryonic development in medaka Oryzias latipes","authors":"Shi Duan,&nbsp;Qing Yang,&nbsp;Fan Wu,&nbsp;Zhenyu Li,&nbsp;Wentao Hong,&nbsp;Mengxi Cao,&nbsp;Xinhua Chen,&nbsp;Xueping Zhong,&nbsp;Qingchun Zhou,&nbsp;Haobin Zhao","doi":"10.1002/jez.2824","DOIUrl":"10.1002/jez.2824","url":null,"abstract":"<p>Methylosome protein 50 (Mep50) is a protein that is rich in WD40 domains, which mediate and regulate a variety of physiological processes in organisms. Previous studies indicated the necessity of Mep50 in embryogenesis in mice <i>Mus musculus</i> and fish. This study aimed to further understand the roles of maternal Mep50 in early embryogenesis using medaka <i>Oryzias latipes</i> as a model. Without maternal Mep50, medaka zygotes developed to the pre-early gastrula stage but died later. The transcriptome of the embryos at the pre-early gastrula stage was analyzed by RNA sequencing. The results indicated that 1572 genes were significantly upregulated and 741 genes were significantly downregulated in the embryos without maternal Mep50. In the differentially expressed genes (DEGs), the DNA-binding proteins, such as histones and members of the small chromosome maintenance complex, were enriched. The major interfered regulatory networks in the embryos losing maternal Mep50 included DNA replication and cell cycle regulation, AP-1 transcription factors such as Jun and Fos, the Wnt pathway, RNA processing, and the extracellular matrix. Quantitative RT-PCR verified 16 DEGs, including <i>prmt5</i>, <i>H2A</i>, <i>cpsf</i>, <i>jun</i>, <i>mcm4</i>, <i>myc</i>, <i>p21</i>, <i>ccne2</i>, <i>cdk6</i>, and <i>col1</i>, among others. It was speculated that the absence of maternal Mep50 could potentially lead to errors in DNA replication and cell cycle arrest, ultimately resulting in cell apoptosis. This eventually resulted in the failure of gastrulation and embryonic death. The results indicate the importance of maternal Mep50 in early embryonic development, particularly in medaka fish.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 7","pages":"798-810"},"PeriodicalIF":1.9,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140668732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted and untargeted metabolomic profiles in wild rabbit does (Oryctolagus cuniculus) of different breeding states (pregnant and lactating)","authors":"Pablo Jesús Marín-García,&nbsp;Carlos Rouco,&nbsp;Lola Llobat,&nbsp;Torben Larsen,&nbsp;Mette Skou Hedemann","doi":"10.1002/jez.2818","DOIUrl":"10.1002/jez.2818","url":null,"abstract":"<p>Ecological nutrition aims to unravel the extensive web of nutritional links that drives animals in their interactions with their ecological environments. Nutrition plays a key role in the success of European wild rabbit (<i>Oryctolagus cuniculus</i>) and could be affected by the breeding status of the animals and reflected in the metabolome of this species. As nutritional needs are considerably increased during pregnancy and lactation, the main objective of this work was to determine how the breeding status (pregnant and lactating) of European wild rabbit does affects nutritional requirements and their metabolome (using targeted and untargeted metabolomics), aiming to find a useful biomarker of breeding status and for monitoring nutritional requirements. To address this gap, 60 wild European rabbits were studied. Animals were divided according to their breeding status and only pregnant (n = 18) and lactating (n = 11) rabbit does were used (n = 29 in total). The body weight and length of each animal were analyzed. The relative and absolute chemical composition of the gastric content and whole blood sample were taken, and targeted and untargeted metabolomics were analyzed. As a main result, there were no differences in biometric measurements, gastric content, and targeted metabolomics, except for live weight and nonesterified fatty acids (NEFA), as pregnant animals showed higher live weight (+12%; <i>p</i> = 0.0234) and lower NEFA acid levels (−46%; <i>p</i> = 0.0262) than lactating females. Regarding untargeted metabolomics, a good differentiation of the metabolome of the two breeding groups was confirmed, and it was proven that pregnant animals showed higher plasmatic levels of succinic anhydride (3.48 more times; <i>p</i> = 0.0236), succinic acid (succinate) (3.1 more times; <i>p</i> = 0.0068) and propionic acid (3.98 more times; <i>p</i> = 0.0121) than lactating animals. However, lactating animals showed higher levels of N-[(3a,5b,7b)-7-hydroxy-24-oxo-3-(sulfoxide) cholan-24-yl]-Glycine (cholestadien) (2.4 more times; <i>p</i> &lt; 0.0420), 4-maleyl-acetoacetate (MAA) (3.2 more times; <i>p</i> &lt; 0.0364) and irilone (2.2 more times; <i>p</i> = 0.0451) than pregnant animals, any of these metabolites could be used as a potential biomarker. From these results, it can be concluded that the most notable changes were observed in the metabolome of individuals, with most of the changes observed being due to energy and protein mobilisation.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 7","pages":"743-752"},"PeriodicalIF":1.9,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jez.2818","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140670644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organization of the blood system of rhynchonellid brachiopod Hemithiris psittacea (Brachiopoda: Rhynchonelliformea)","authors":"Tatyana Kuzmina,&nbsp;Vladislav Kuzmin,&nbsp;Ekaterina Bogomolova,&nbsp;Vladimir Malakhov","doi":"10.1002/jez.2820","DOIUrl":"10.1002/jez.2820","url":null,"abstract":"<p>The brachiopods are sessile invertebrates with an unusual blood system, which consists of a long-branched dorsal vessel. It is still unknown how blood circulates in this system. In the present study, for the first time we propose the circulation of blood in brachiopod <i>Hemithiris psittacea</i> based on morphological and experimental data. The main heart is located on the dorsal side of the stomach and divides the dorsal vessel into anterior and posterior parts. The anterior part enters the lophophore, where it gives off blind branches to each tentacle. The posterior part passes by the funnels of the nephridia and forms a blindly closed network in the gonads. We suggest that the circulation of blood includes three successive stages. During the first phase of systole of the main heart, blood flows through the anterior dorsal vessel. During the second phase of systole, blood flows through the posterior dorsal vessel. During diastole, blood flows from the anterior and posterior vessels and fills the main heart. The origin of a peculiar blood system in brachiopods can be explained by reduction of the ventral vessel, which is probably correlates with the reduction of the ventral side of the brachiopod ancestor's body. Another peculiarity of brachiopod blood system is the presence of an ampullar heart, which functions as a blood depot and allows blood to move in the vessels in two directions in an oscillatory mode. The brachiopod blood system contains vessels lacking true endothelium and can be classified as an “incompletely closed” type.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 7","pages":"766-782"},"PeriodicalIF":1.9,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140670597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complexity of trophic relationships between Gammarus aequicauda (Amphipoda) and Eucypris mareotica (Ostracoda) in a hypersaline lake","authors":"Nickolai Shadrin,&nbsp;Vladimir Yakovenko,&nbsp;Elena Anufriieva","doi":"10.1002/jez.2821","DOIUrl":"10.1002/jez.2821","url":null,"abstract":"<p>In the hypersaline lakes of Crimea, only 5–7 species of animals are common including <i>Gammarus aequicauda</i> (Amphipoda) and <i>Eucypris mareotica</i> (Ostracoda). Two experiments were carried out to characterize the trophic interactions between these species. In one, <i>G. aequicauda</i> were used freshly caught from the lake, and in the other, after they were starved for 2.5 days in the laboratory. The experimental options were one male or female <i>G. aequicauda</i> and either 10, 20, or 30 ostracods. The gammarids actively fed on the ostracods, and significant differences were between females and males. The feeding intensity of males was higher than that of females. However, ostracods are not passive prey, they can actively attack their predator and are capable of causing them harm. Ostracods attacked a Gammarus only if it had captured one of the ostracods. Sex differences in <i>G. aequicauda</i> were also evident in the reaction to the group's aggressive behavior of ostracods. Interactions between Ostracoda and Gammarids cannot be considered unidirectional; they both may be prey or predators depending on the situation.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 6","pages":"717-726"},"PeriodicalIF":2.8,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140630870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drosophila melanogaster as a model organism for diabetes II treatment by the ethyl acetate fraction of Atriplex halimus L.","authors":"Omnia Montaser,&nbsp;Mona El-Aasr,&nbsp;Haytham O. Tawfik,&nbsp;Wesam S. Meshrif,&nbsp;Hanaa Elbrense","doi":"10.1002/jez.2812","DOIUrl":"10.1002/jez.2812","url":null,"abstract":"<p>Type 2 diabetes (T2D) is the most common metabolic disorder. The undesirable effects of synthetic drugs demand a search for safe antidiabetic agents. This study aimed to assess the antidiabetic activity of different fractions of <i>Atriplex halimus</i> (petroleum ether 60–80, methylene chloride, ethyl acetate, and <i>n</i>-butanol) using <i>Drosophila melanogaster</i> larvae. Titers of total glucose and trehalose, as well as larval weight, were measured and compared with those of control and diabetic larvae. The expression of <i>Drosophila</i> insulin-like peptides (DILP2 and DILP3) and adipokinetic hormone (AKH) was evaluated. The results revealed a significant increase in total glucose, trehalose, and a decrease in body weight in the larvae fed a high-sugar diet compared with those in the control. When larvae fed diets containing the tested fractions, the total glucose and trehalose decreased to the control level, and the body weight increased. DILP2, DILP3, and AKH exhibited significant decreases upon treatment with <i>A. halimus</i> ethyl acetate. Metabolomic profiling of the ethyl acetate fraction of <i>A. halimus</i> revealed the presence of flavonoids and flavonoid glycosides. After docking screening to predict the most powerful moiety, we discovered that flavonoid glycosides (especially eriodictyol-7-<i>O</i>-neohesperidoside) have a greater affinity for the pocket than the other moieties. The results indicated the therapeutic activity of the <i>A. halimus</i> ethyl acetate fraction against induced T2D in <i>Drosophila</i> larvae. The antidiabetic activity may be attributed to flavonoids, which are the main components of the <i>A. halimus</i> ethyl acetate fraction.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 6","pages":"702-716"},"PeriodicalIF":2.8,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140578283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short-term immunotoxicity induced by florpyrauxifen-benzyl herbicide exposure in the spleen of male albino mice","authors":"Dalia S. Morsi,&nbsp;Hagar A. Abu Quora,&nbsp;Hind S. AboShabaan,&nbsp;Islam M. El-Garawani,&nbsp;Ahmed E. Abdel Moneim,&nbsp;Heba M. R. Hathout","doi":"10.1002/jez.2814","DOIUrl":"10.1002/jez.2814","url":null,"abstract":"<p>Florpyrauxifen-benzyl (FPB) is a new arylpicolinate systemic herbicide that has been used to control or suppress the majority of herbicide-resistant biotype weeds in rice. To our knowledge, the impact of FPB on the immune system remains undetected thus far. Hence, this work aimed to address the toxic effects of FPB and the possible related mechanisms on the spleen of exposed mice. Initially, an acute toxicological test was performed to ascertain the median lethal dose (LD₅₀) of FPB for 24 h which was found to be 371.54 mg/kg b.wt. For mechanistic evaluation of FPB toxicity, three sublethal doses (1/20^th, 1/10^th, and 1/5^th LD₅₀) were orally administered to mice for 21 consecutive days. Changes in spleen relative weight, oxidative status, apoptotic and inflammatory markers, histopathological alterations were evaluated. Following the FPB exposure, significant (<i>p</i> &lt; 0.05) decline in spleen index, apoptotic features, histolopathological changes were observed. Additionally, excessive oxidative stress in spleen tissues was monitored by downregulating antioxidant enzymes and upregulating the oxidant parameters. Furthermore, exposure to FPB resulted in notable activation of the NF-қB signaling pathway, accompanied by elevated levels of pro-inflammatory cytokines (namely, IL-1β and TNF-α) as well as CD3 and CD19 levels have decreased significantly in spleen tissues. Collectively, FPB exposure exhibited apoptosis, oxidative stress, immunosuppression, and inflammatory response in a dose-dependent manner, leading to spleen tissue damage and immunotoxicity. Further studies on FPB is recommended to outstand its hazards on ecosystems.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 6","pages":"658-671"},"PeriodicalIF":2.8,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140578280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic effect of N, N-Diphenyl-1,4-phenylenediamine and adipose-derived stem cells coadministration on diabetic cardiomyopathy in type 1 diabetes mellitus-rat model","authors":"Hany M. Abd El-lateef,&nbsp;Lashin S. Ali,&nbsp;Safa H. Qahl,&nbsp;Dalal N. Binjawhar,&nbsp;Eman Fayad,&nbsp;Maha A. Alghamdi,&nbsp;Sarah A. Altalhi,&nbsp;Fawziah A. Al-Salmi,&nbsp;El Shaimaa Shabana,&nbsp;Kholoud H. Radwan,&nbsp;Ibrahim Youssef,&nbsp;Saad Shaaban,&nbsp;Hanan M. Rashwan,&nbsp;Shady G. El-Sawah","doi":"10.1002/jez.2810","DOIUrl":"10.1002/jez.2810","url":null,"abstract":"<p>Type 1 diabetes stem-cell-based treatment approach is among the leading therapeutic strategies for treating cardiac damage owing to the stem cells' regeneration capabilities. Mesenchymal stem cells derived from adipose tissue (AD-MSCs) have shown great potential in treating diabetic cardiomyopathy (DCM). Herein, we explored the antioxidant-supporting role of N, N′-diphenyl-1,4-phenylenediamine (DPPD) in enhancing the MSCs' therapeutic role in alleviating DCM complications in heart tissues of type 1 diabetic rats. Six male albinos Wistar rat groups have been designed into the control group, DPPD (250 mg/kg, i.p.) group, diabetic-untreated group, and three diabetic rat groups treated with either AD-MSCs (1 × 10⁶ cell/rat, i.v.) or DPPD or both. Interestingly, all three treated diabetic groups exhibited a significant decrease in serum glucose, HbA1c, heart dysfunction markers (lactate dehydrogenase and CK-MP) levels, and lipid profile fractions (except for HDL-C), as well as some cardiac oxidative stress (OS) levels (MDA, AGEs, XO, and ROS). On the contrary, serum insulin, C-peptide, and various cardiac antioxidant levels (GSH, GST, CAT, SOD, TAC, and HO-1), beside viable cardiac cells (G0/G1%), were markedly elevated compared with the diabetic untreated group. In support of these findings, the histological assay reflected a marked enhancement in the cardiac tissues of all diabetic-treated groups, with obvious excellency of the AD-MSCs + DPPD diabetic-treated group. Such results strongly suggested the great therapeutic potentiality of either DPPD or AD-MSCs single injection in enhancing the cardiac function of diabetic rats, with a great noted enhancement superiority of DPPD and AD-MSCs coadministration.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 6","pages":"647-657"},"PeriodicalIF":2.8,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140578381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selenium suppressed growth of Ehrlich solid tumor and improved health of tumor-bearing mice","authors":"Heba A. Hekal,&nbsp;Maggie E. Amer,&nbsp;Maher Amer,&nbsp;Mohamed A. El-Missiry,&nbsp;Azza I. Othman","doi":"10.1002/jez.2815","DOIUrl":"10.1002/jez.2815","url":null,"abstract":"<p>Selenium (Se) is an important micronutritional biomolecule in cancer therapy. The current work evaluated the anticancer effect of Se and its ability to improve health of mice with solid Ehrlich carcinoma implanted subcutaneously. Four groups of five female BALB/c mice each were assembled. Ehrlich tumor cells were engrafted into two of them, either with or without Se therapy. The other groups served as control groups, either with or without Se treatment. Se treatment resulted in a notable decrease in both tumor volume and animal body mass in tumor-bearing mice. Treatment with Se markedly increased oxidative stress in tumor while ameliorating oxidative stress in sera of tumors-bearing mice. Similarly, treatment with Se resulted in downregulation of inflammatory cytokines (TNF-α and IL-6) while increasing IL-10 in serum of tumor-bearing mice. Conversely, selenium increased TNF- α and IL-6 and decreased IL-10 in tumor suggesting disruption of tumor immunity. The increased oxidative stress and inflammation in tumor tissue dysregulated cell cycle phases with increase apoptotic tumor cells population in G₀/G₁ phase. This is supported by the increased levels apoptotic regulating proteins (Bax and caspase-3 and P-53) while decreasing Bcl-2 in the tumor tissue. Treatment with Se also resulted in increased comet parameters indicating DNA damage of tumor cells. Histopathological examination revealed a significant decrease in a number of neoplastic cells within tumor of mice that treated with Se. In conclusion, these findings suggest that Se therapy significantly suppressed solid tumor proliferation and growth while mitigating the health status of tumor-bearing mice.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 6","pages":"672-682"},"PeriodicalIF":2.8,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140578386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}