Journal of experimental zoology. Part A, Ecological and integrative physiology最新文献

{"title":"Bridging the buzz: In vivo EPR imaging unlocking the secrets of honey bee health","authors":"Đura Nakarada,&nbsp;Uroš Glavinić,&nbsp;Marko Ristanić,&nbsp;Milan Popović,&nbsp;Jevrosima Stevanović,&nbsp;Zoran Stanimirović,&nbsp;Miloš Mojović","doi":"10.1002/jez.2845","DOIUrl":"10.1002/jez.2845","url":null,"abstract":"<p>Honey bees play a pivotal role in shaping ecosystems and sustaining human health as both pollinators and producers of health-promoting products. However, honey bee colony mortality is on the rise globally, driven by various factors, including parasites, pesticides, habitat loss, poor nutrition, and climate change. This has far-reaching consequences for the environment, economy, and human welfare. While efforts to address these issues are underway, the current progress in electron paramagnetic resonance (EPR) instrumentation affords using the immense potential of this magnetic resonance technique to study small samples such as honey bees. This paper presents the pioneering 2D in vivo EPR imaging experiment on a honey bee, revealing the ongoing redox-status of bees’ intestines. This way, by monitoring the spatio-temporal changes of the redox-active spin-probes’ EPR signal, it is possible to gain access to valuable information on the course of ongoing bees’ pathologies and the prospect of following-up on the efficiency of applied therapies. Employing a selection of diverse spin-probes could further reveal pH levels and oxygen concentrations in bee tissues, allowing a noninvasive assessment of bee physiology. This approach offers promising strategies for safeguarding pollinators and understanding their biology, fostering their well-being and ecological harmony.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 8","pages":"880-884"},"PeriodicalIF":1.9,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141457353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation for oxidative stress in Chinese toads (Bufo gargarizans) living under natural conditions along an altitudinal gradient","authors":"Haiying Zhang,&nbsp;Tisen Xu,&nbsp;Mingxue Jiao,&nbsp;Xiangyong Li,&nbsp;Kenneth B. Storey,&nbsp;Yonggang Niu","doi":"10.1002/jez.2842","DOIUrl":"10.1002/jez.2842","url":null,"abstract":"<p>Preparation for oxidative stress (POS) has been widely reported in animals under controlled laboratory conditions, but whether this phenomenon is visible in animals under natural conditions remains to be explored. Altitudinal gradients provide a good opportunity to address this question, since environmental conditions become more hostile with increasing altitude. Here, we investigated the levels of oxidative stress, oxidative damage, and antioxidant defenses in Chinese toads (<i>Bufo gargarizans</i>) along an altitudinal gradient (50 m, 1200 m, 2300 m, 3400 m above sea level). The results show that changing altitude led to a significantly lower ratio of oxidized to reduced glutathione in liver, with a higher value at 50 m. This ratio in muscle tissues did not differ significantly between altitudes of 50 m, 2300 m, and 3400 m. However, reduced glutathione content increased significantly along the altitude, with higher values in liver at 2300 m and higher values in skeletal muscle at 3400 m. Malondialdehyde (MDA) content in liver did not change significantly with increasing altitude. Brain and muscle tissues showed a higher MDA content at 50 m than the other three altitudes. The activities of superoxide dismutase, catalase, glutathione peroxidase, and glutathione-S-transferase, as well as total antioxidant capacity, also displayed tissue-specific upregulation in heart, skeletal muscle, and brain, but all of these antioxidant enzymes except for glutathione-S-transferase were significantly reduced in liver along the altitudinal gradient. In summary, environmental factors at higher altitude did not lead to higher levels of oxidative stress and oxidative damage in <i>B. gargarizans</i>, mainly due to stronger antioxidant defenses. This study corroborates the occurrence of POS in high-altitude toads living under field conditions and contributes to revealing the biochemical adaptations to extreme environments at higher altitude.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 8","pages":"867-879"},"PeriodicalIF":1.9,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141457355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gill morphology adapted to oxygen-limited caves in Astyanax mexicanus","authors":"Tyler E. Boggs,&nbsp;Joshua B. Gross","doi":"10.1002/jez.2840","DOIUrl":"10.1002/jez.2840","url":null,"abstract":"<p>Sensing and acquiring dissolved oxygen is crucial for nearly all aquatic life. This may become even more vital as dissolved oxygen concentrations continue to decline in many aquatic environments. While certain phenotypes that enable fish to live in low oxygen have been characterized, adaptations that arise following sudden, drastic reductions in dissolved oxygen are relatively unknown. Here, we assessed the blind Mexican cavefish, <i>Astyanax mexicanus</i>, for alterations to gill morphology that may be adaptive for life in hypoxic caves. The <i>Astyanax</i> system provides the unique opportunity to compare gill morphology between stereotypical “surface” adapted morphotypes and obligate cave-dwelling conspecifics. While the surface environment is well-oxygenated, cavefish must cope with significantly reduced oxygen. We began by quantifying traditional morphological gill traits including filament number and length as well as lamellar density and height in surface fish and two distinct cave populations, Pachón and Tinaja. This enabled us to estimate total lamellar height, a proxy for gill surface area. We then used immunohistochemical staining to label 5-HT-positive neuroepithelial cells (NECs), which serve as key oxygen sensors in fish. We discovered an increase in gill surface area for both cavefish populations compared to surface, which may enable a higher capacity of oxygen acquisition. Additionally, we found more NECs in Pachón cavefish compared to both surface fish and Tinaja cavefish, suggesting certain selective pressures may be cave-specific. Collectively, this work provides evidence that cavefish have adapted to low oxygen conditions via alterations to gill morphology and oxygen sensing, and informs evolutionary mechanisms of rapid adaptation to dramatic, chronic hypoxia.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 8","pages":"856-866"},"PeriodicalIF":1.9,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jez.2840","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141506489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization and expression analysis of thyroid hormone receptors in protogynous rice field eel, Monopterus albus","authors":"Ke Feng,&nbsp;Jialin Su,&nbsp;Lei Sun,&nbsp;Ying Guo,&nbsp;Xiwen Peng","doi":"10.1002/jez.2825","DOIUrl":"10.1002/jez.2825","url":null,"abstract":"<p>Thyroid hormones (THs) play important roles in growth, development, morphogenesis, reproduction, and so on. They are mainly meditated by binding to thyroid hormone receptors (TRs) in vertebrates. As important members of the nuclear receptor superfamily, TRs and their ligands are involved in many biological processes. To investigate the potential roles of TRs in the gonadal differentiation and sex change, we cloned and characterized the TRs genes in protogynous rice field eel (<i>Monopterus albus</i>). In this study, three types of TRs were obtained, which were TRαA, TRαB and TRβ, encoding preproproteins of 336-, 409- and 415-amino acids, respectively. Multiple alignments of the three putative TRs protein sequences showed they had a higher similarity. Tissue expression analysis showed that TRαA mainly expressed in the gonad, while TRαB and TRβ in the brain. During female-to-male sex reversal, the expression levels of all the three TRs showed a similar trend of increase followed by a decrease in the gonad. Intraperitoneal injection of triiodothyronine (T3) stimulated the expression of TRαA and TRαB, while it had no significant change on the expression of TRβ in the ovary. Gonadotropin-releasing hormone analogue (GnRHa) injection also significantly upregulated the expression levels of TRαA and TRαB after 6 h, while it had no significant effect on TRβ. These results demonstrated that TRs were involved in the gonadal differentiation and sex reversal, and TRα may play more important roles than TRβ in reproduction by the regulation of GnRHa in rice field eel.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 8","pages":"845-855"},"PeriodicalIF":1.9,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141296182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sleep in the East African root rat, Tachyoryctes splendens","authors":"Jean-Leigh Kruger,&nbsp;Adhil Bhagwandin,&nbsp;Jestina V. Katandukila,&nbsp;Nigel C. Bennett,&nbsp;Paul R. Manger","doi":"10.1002/jez.2839","DOIUrl":"10.1002/jez.2839","url":null,"abstract":"<p>The present study reports the results of an electrophysiological analysis of sleep in the East African root rat, <i>Tachyoryctes splendens</i>, belonging to the rodent subfamily <i>Spalacinae</i>. Telemetric electroencephalographic (EEG) and electromyographic recordings, with associated video recording, on three root rats over a continuous 72 h period (12 h light/12 h dark cycle) were analyzed. The analysis revealed that the East African root rat has a total sleep time (TST) of 8.9 h per day. Despite this relatively short total sleep time in comparison to fossorial rodents, nonrapid eye movement (non-REM) sleep and rapid eye movement (REM) sleep states showed similar physiological signatures to that observed in other rodents and no unusual sleep states were observed. REM occupied 19.7% of TST, which is within the range observed in other rodents. The root rats were extremely active during the dark period, and appeared to spend much of the light period in quiet wake while maintaining vigilance (as determined from both EEG recordings and behavioral observation). These recordings were made under normocapnic environmental conditions, which contrasts with the hypercapnic environment of their natural burrows.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 10","pages":"1111-1120"},"PeriodicalIF":1.9,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11579819/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141199084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxygen consumption rate of flatworms under the influence of wake- and sleep-promoting neurotransmitters","authors":"Shauni E. T. Omond,&nbsp;Robert G. Barker,&nbsp;Oana Sanislav,&nbsp;Paul R. Fisher,&nbsp;Sarah J. Annesley,&nbsp;John A. Lesku","doi":"10.1002/jez.2828","DOIUrl":"10.1002/jez.2828","url":null,"abstract":"<p>Flatworms are among the best studied animal models for regeneration; however, they also represent an emerging opportunity to investigate other biological processes as well. For instance, flatworms are nocturnal and sleep during the day, a state that is regulated by sleep/wake history and the action of the sleep-promoting neurotransmitter gamma-aminobutyric acid (or GABA). Sleep is widespread across the animal kingdom, where it serves many nonexclusive functions. Notably, sleep saves energy by reducing metabolic rate and by not doing something more energetically taxing. Whether the conservation of energy is apparent in sleeping flatworms is unclear. We measured the oxygen consumption rate (OCR) of flatworms dosed with either (<i>1</i>) GABA (<i>n</i> = 29) which makes flatworms inactive or (<i>2</i>) dopamine (<i>n</i> = 20) which stimulates flatworms to move, or (<i>3</i>) day and night neurotransmitter-free controls (<i>n</i> = 28 and 27, respectively). While OCR did not differ between the day and night, flatworms treated with GABA used less oxygen than those treated with dopamine, and less than the day-time control. Thus, GABA affected flatworm physiology, ostensibly by enforcing energy-conserving sleep. Evidence that dopamine increased metabolism was less strong. This work broadens our understanding of flatworm physiology and expands the phylogenetic applicability of energy conservation as a function of sleep.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 10","pages":"1130-1136"},"PeriodicalIF":1.9,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11579807/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141154850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intraspecific variation in thermal performance curves for early development in Fundulus heteroclitus","authors":"Tessa S. Blanchard,&nbsp;Madison L. Earhart,&nbsp;Ariel K. Shatsky,&nbsp;Patricia M. Schulte","doi":"10.1002/jez.2827","DOIUrl":"10.1002/jez.2827","url":null,"abstract":"<p>Thermal performance curves (TPCs) provide a framework for understanding the effects of temperature on ectotherm performance and fitness. TPCs are often used to test hypotheses regarding local adaptation to temperature or to develop predictions for how organisms will respond to climate warming. However, for aquatic organisms such as fishes, most TPCs have been estimated for adult life stages, and little is known about the shape of TPCs or the potential for thermal adaptation at sensitive embryonic life stages. To examine how latitudinal gradients shape TPCs at early life stages in fishes, we used two populations of <i>Fundulus heteroclitus</i> that have been shown to exhibit latitudinal variation along the thermal cline as adults. We exposed embryos from both northern and southern populations and their reciprocal crosses to eight different temperatures (15°C, 18°C, 21°C, 24°C, 27°C, 30°C, 33°C, and 36°C) until hatch and examined the effects of developmental temperature on embryonic and larval traits (shape of TPCs, heart rate, and body size). We found that the pure southern embryos had a right-shifted TPC (higher thermal optimum (<i>T</i>_opt) for developmental rate, survival, and embryonic growth rate) whereas pure northern embryos had a vertically shifted TPC (higher maximum performance (<i>P</i>_max) for developmental rate). Differences across larval traits and cross-type were also found, such that northern crosses hatched faster and hatched at a smaller size compared to the pure southern population. Overall, these observed differences in embryonic and larval traits are consistent with patterns of both local adaptation and countergradient variation.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 8","pages":"827-844"},"PeriodicalIF":1.9,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jez.2827","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141071073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction to “Estrogen-regulated expression of P450arom genes in the brain and ovaries of adult female Indian climbing perch, Anabas testudineu”","authors":"","doi":"10.1002/jez.2826","DOIUrl":"10.1002/jez.2826","url":null,"abstract":"<p>Retraction: Pal, P., Moulik, S. R., Gupta, S., Guha, P., Majumder, S., Kundu, S., Mallick, B., Pramanick, K., Mukherjee, D. (2018). Estrogen-regulated expression of P450arom genes in the brain and ovaries of adult female Indian climbing perch, <i>Anabas testudineu. Journal of Experimental Zoology – A</i> 329, no. 1, 29–42. https://doi.org/10.1002/jez.2158.</p><p>The above article, published online on 18 April 2018 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement among the journal Editor-in-Chief, Randy Nelson, and Wiley Periodicals LLC. The retraction has been agreed upon following an investigation into concerns raised by a third party, which revealed inappropriate duplication, splicing, resizing, and insertion in Figures 1, 2, 3, 4, and 6A,B. Given the extent of the identified issues, the editors have lost confidence in the data presented and have therefore decided to retract it. The authors were informed of the decision to retract but did not agree to the retraction.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 6","pages":"727"},"PeriodicalIF":2.8,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jez.2826","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140944425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temperature regulates sex determination and growth in the paralichthid flatfish California halibut","authors":"Ruairi MacNamara,&nbsp;Jamie L. Mankiewicz,&nbsp;Scott A. Salger,&nbsp;Kevin Stuart,&nbsp;Russell J. Borski,&nbsp;John Godwin,&nbsp;Mark Drawbridge","doi":"10.1002/jez.2823","DOIUrl":"10.1002/jez.2823","url":null,"abstract":"<p>California halibut (<i>Paralichthys californicus</i>) is a candidate species for aquaculture and stock enhancement. These applications rely on sex control, either to maximize the production of faster growing females or to match sex ratios in the wild. Other paralichthids exhibit temperature-dependent sex determination (TSD), but the presence and pattern of TSD is not well defined in California halibut. Juvenile California halibut were cultured at three distinct temperatures (15°C, 19°C, and 23°C) through the developmental period presumed to be thermosensitive based on findings from congeners. Sex ratios were quantified in each treatment using phenotypic sex identification techniques applied early (molecular biomarkers; 51–100 mm total length [TL]) and late (visual examination of the gonads; ≥100 mm TL) in the juvenile phase. Both techniques indicated similar sex determination trends at each temperature, with overall sex ratios assessed as 49.9% male at 15°C, 74.5% male at 19°C, and 98.2% male at 23°C. Growth rates were highest at 23°C and lowest at 15°C, with intrinsically fast- and slow-growing individuals at all temperatures. At 15°C and 19°C, females comprised a higher proportion among the fast growers than they did among the slow growers. These data show that California halibut exhibit TSD, with temperatures of 19°C and 23°C masculinizing fish while 15°C appears to produce a 1:1 sex ratio. This study will help optimize sex ratios and growth in hatcheries through thermal manipulation. Furthermore, the developed biomolecular tools and identified temperature thresholds will be important in future work to understand the influence of global warming on wild population demographics.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 7","pages":"811-821"},"PeriodicalIF":1.9,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140839074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of phenotypic plasticity and corticosterone in coping with pond drying conditions in yellow-bellied toad (Bombina variegata, Linnaeus 1758) tadpoles","authors":"Ana Kijanović,&nbsp;Tanja Vukov,&nbsp;Marko Mirč,&nbsp;Aleksandar Mitrović,&nbsp;Marko D. Prokić,&nbsp;Tamara G. Petrović,&nbsp;Tijana B. Radovanović,&nbsp;Branka R. Gavrilović,&nbsp;Svetlana G. Despotović,&nbsp;Jelena P. Gavrić,&nbsp;Nataša Tomašević Kolarov","doi":"10.1002/jez.2819","DOIUrl":"10.1002/jez.2819","url":null,"abstract":"<p>Amphibian larvae inhabiting temporary ponds often exhibit the capacity to accelerate development and undergo metamorphosis in challenging conditions like desiccation. However, not all species exhibit this ability, the yellow-bellied toad (<i>Bombina variegata)</i> is one such example. The underlying mechanisms behind the inability to accelerate development under desiccation remain largely unexplored. The hypothalamic–pituitary–interrenal (HPI) axis and corticosterone (CORT), which act synergistically with thyroid hormone, are thought to facilitate metamorphosis in response to desiccation stress. In this study, we aimed to investigate whether modification in the HPI axis, particularly CORT levels, contributes to the absence of adaptive plasticity in <i>B. variegata</i> under desiccation stress. The study design included four treatments: high water level, high water level with exogenous CORT, low water level, and low water level with metyrapone (a CORT synthesis inhibitor). The main objective was to evaluate the effects of these treatments on whole-body corticosterone levels, life history, morphological traits, and oxidative stress parameters during the prometamorphic and metamorphic climax developmental stages. While low water level had no effect on total corticosterone levels, larval period, body condition index, and metamorphic body shape, it negatively affected metamorph size, mass, and growth rate. Our findings suggest that constant exposure to desiccation stress over generations may have led to modifications in the HPI axis activity in <i>B. variegata</i>, resulting in adaptation to changes in water level, evident through the absence of stress response. Consequently, CORT may not be a relevant stress indicator in desiccation conditions for this species.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 7","pages":"753-765"},"PeriodicalIF":1.9,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jez.2819","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140667759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}