Journal of experimental zoology. Part A, Ecological and integrative physiology最新文献

{"title":"Retraction to “Estrogen-regulated expression of P450arom genes in the brain and ovaries of adult female Indian climbing perch, Anabas testudineu”","authors":"","doi":"10.1002/jez.2826","DOIUrl":"10.1002/jez.2826","url":null,"abstract":"<p>Retraction: Pal, P., Moulik, S. R., Gupta, S., Guha, P., Majumder, S., Kundu, S., Mallick, B., Pramanick, K., Mukherjee, D. (2018). Estrogen-regulated expression of P450arom genes in the brain and ovaries of adult female Indian climbing perch, <i>Anabas testudineu. Journal of Experimental Zoology – A</i> 329, no. 1, 29–42. https://doi.org/10.1002/jez.2158.</p><p>The above article, published online on 18 April 2018 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement among the journal Editor-in-Chief, Randy Nelson, and Wiley Periodicals LLC. The retraction has been agreed upon following an investigation into concerns raised by a third party, which revealed inappropriate duplication, splicing, resizing, and insertion in Figures 1, 2, 3, 4, and 6A,B. Given the extent of the identified issues, the editors have lost confidence in the data presented and have therefore decided to retract it. The authors were informed of the decision to retract but did not agree to the retraction.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 6","pages":"727"},"PeriodicalIF":2.8,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jez.2826","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140944425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temperature regulates sex determination and growth in the paralichthid flatfish California halibut","authors":"Ruairi MacNamara,&nbsp;Jamie L. Mankiewicz,&nbsp;Scott A. Salger,&nbsp;Kevin Stuart,&nbsp;Russell J. Borski,&nbsp;John Godwin,&nbsp;Mark Drawbridge","doi":"10.1002/jez.2823","DOIUrl":"10.1002/jez.2823","url":null,"abstract":"<p>California halibut (<i>Paralichthys californicus</i>) is a candidate species for aquaculture and stock enhancement. These applications rely on sex control, either to maximize the production of faster growing females or to match sex ratios in the wild. Other paralichthids exhibit temperature-dependent sex determination (TSD), but the presence and pattern of TSD is not well defined in California halibut. Juvenile California halibut were cultured at three distinct temperatures (15°C, 19°C, and 23°C) through the developmental period presumed to be thermosensitive based on findings from congeners. Sex ratios were quantified in each treatment using phenotypic sex identification techniques applied early (molecular biomarkers; 51–100 mm total length [TL]) and late (visual examination of the gonads; ≥100 mm TL) in the juvenile phase. Both techniques indicated similar sex determination trends at each temperature, with overall sex ratios assessed as 49.9% male at 15°C, 74.5% male at 19°C, and 98.2% male at 23°C. Growth rates were highest at 23°C and lowest at 15°C, with intrinsically fast- and slow-growing individuals at all temperatures. At 15°C and 19°C, females comprised a higher proportion among the fast growers than they did among the slow growers. These data show that California halibut exhibit TSD, with temperatures of 19°C and 23°C masculinizing fish while 15°C appears to produce a 1:1 sex ratio. This study will help optimize sex ratios and growth in hatcheries through thermal manipulation. Furthermore, the developed biomolecular tools and identified temperature thresholds will be important in future work to understand the influence of global warming on wild population demographics.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 7","pages":"811-821"},"PeriodicalIF":1.9,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140839074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of phenotypic plasticity and corticosterone in coping with pond drying conditions in yellow-bellied toad (Bombina variegata, Linnaeus 1758) tadpoles","authors":"Ana Kijanović,&nbsp;Tanja Vukov,&nbsp;Marko Mirč,&nbsp;Aleksandar Mitrović,&nbsp;Marko D. Prokić,&nbsp;Tamara G. Petrović,&nbsp;Tijana B. Radovanović,&nbsp;Branka R. Gavrilović,&nbsp;Svetlana G. Despotović,&nbsp;Jelena P. Gavrić,&nbsp;Nataša Tomašević Kolarov","doi":"10.1002/jez.2819","DOIUrl":"10.1002/jez.2819","url":null,"abstract":"<p>Amphibian larvae inhabiting temporary ponds often exhibit the capacity to accelerate development and undergo metamorphosis in challenging conditions like desiccation. However, not all species exhibit this ability, the yellow-bellied toad (<i>Bombina variegata)</i> is one such example. The underlying mechanisms behind the inability to accelerate development under desiccation remain largely unexplored. The hypothalamic–pituitary–interrenal (HPI) axis and corticosterone (CORT), which act synergistically with thyroid hormone, are thought to facilitate metamorphosis in response to desiccation stress. In this study, we aimed to investigate whether modification in the HPI axis, particularly CORT levels, contributes to the absence of adaptive plasticity in <i>B. variegata</i> under desiccation stress. The study design included four treatments: high water level, high water level with exogenous CORT, low water level, and low water level with metyrapone (a CORT synthesis inhibitor). The main objective was to evaluate the effects of these treatments on whole-body corticosterone levels, life history, morphological traits, and oxidative stress parameters during the prometamorphic and metamorphic climax developmental stages. While low water level had no effect on total corticosterone levels, larval period, body condition index, and metamorphic body shape, it negatively affected metamorph size, mass, and growth rate. Our findings suggest that constant exposure to desiccation stress over generations may have led to modifications in the HPI axis activity in <i>B. variegata</i>, resulting in adaptation to changes in water level, evident through the absence of stress response. Consequently, CORT may not be a relevant stress indicator in desiccation conditions for this species.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 7","pages":"753-765"},"PeriodicalIF":1.9,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jez.2819","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140667759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Macro- and micro-morphological comparison of the detailed structure of the oral cavity roof in two different feeding habits marine fishes: Pagrus pagrus and Boops boops","authors":"Mohamed A. M. Alsafy,&nbsp;Manal A. Seif,&nbsp;Ahmed A. El-Mansi,&nbsp;Mohamed El-Beskawy,&nbsp;Mohamed El Dakroury,&nbsp;Mamdouh B. Eldesoqui,&nbsp;Samar M. Ez Elarab,&nbsp;Samir A. A. El-Gendy","doi":"10.1002/jez.2822","DOIUrl":"10.1002/jez.2822","url":null,"abstract":"<p>The feeding habits and habitats of fish influence the morphology of the oral cavity. This study used gross anatomy, light microscopy, and scanning electron microscopy, in addition to morphometric analysis, to investigate the anatomical characteristics of the oral cavity roof in <i>Pagrus pagrus</i> and <i>Boops boops</i>, which have different dietary habits. The oral cavity roof appeared U-shaped and divided into the palate and upper pharyngeal regions. The upper lip of <i>P. pagrus</i> was broad, while <i>B. boops'</i> upper lip was small and thin. Both species had a stratified squamous epithelium with an irregular shape and a folded surface. <i>P. pagrus</i> had a horseshoe-shaped upper velum with a high middle part, and its surface resembled sea waves with obvious mucous-secreting openings with cilia and many folds and grooves between them. <i>B. boops's</i> upper velum was thin and appeared as a triangle pouch with a pointed cranial apex. The palate in both species was narrow in the front and increased in width backward until it ended. The upper pharyngeal teeth in <i>P. pagrus</i> appeared as two patches, separated by a median longitudinal ridge and an anterior V-shape separator. Meanwhile, in <i>B. boops</i>, they appeared as a ball patch on both sides and a separator ridge in the middle. Because <i>P. pagrus</i> fed on harder structures than <i>B. boops</i>, their feeding habits were reflected in the structure of the oral cavity roof. <i>P. pagrus</i>, a carnivorous species, had several rows of sharp upper jaw and upper pharyngeal teeth, thick spinous tubercles on oblique transverse ridges, and massive mucous glands. On the other hand, <i>B. boops</i>, an omnivorous species, had only one row of upper jaw teeth, a few upper pharyngeal teeth scattered on two oval patches, and thin filaments on the oblique transverse ridges.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 7","pages":"783-797"},"PeriodicalIF":1.9,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140665838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimentally elevated corticosterone increases song output and complexity in common mynas","authors":"Juliane Gaviraghi Mussoi,&nbsp;Rebecca A. MacQueen,&nbsp;Margaret C. Stanley,&nbsp;Kristal E. Cain","doi":"10.1002/jez.2817","DOIUrl":"10.1002/jez.2817","url":null,"abstract":"<p>Vocalization is an important communication tool that can reflect many aspects of an individual's internal and external condition. This is especially true for birds. Previous research has shown that bird calls and songs change in response to a variety of potential stressors, although the extent and direction of the changes depend on the nature of the stressor and the environment. Circulating glucocorticoids, such as corticosterone, often increase in response to stressors and mediate some of the observed changes via alterations of the individual's physiological state. Acute elevations of corticosterone often occur as a physiological response to short-term stressors; however, the effects of this elevation on adult vocalizations have not been well documented. Here, we experimentally elevated corticosterone at two different levels using a noninvasive method and examined the effects on the vocal communication of male and female adult common mynas (<i>Acridotheres tristis</i>). Corticosterone elevation temporarily increased song output and some measures of song complexity, while call output decreased. These effects were dosage dependent (higher corticosterone levels had a stronger effect), most evident 40 min after ingestion, and some vocal changes were sex-specific. Future studies should investigate whether the changes in vocal performance due to elevated glucocorticoids have consequences for the birds' behavior, reproductive success, and survival.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 7","pages":"733-742"},"PeriodicalIF":1.9,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jez.2817","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140666625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal methylosome protein 50 is essential for embryonic development in medaka Oryzias latipes","authors":"Shi Duan,&nbsp;Qing Yang,&nbsp;Fan Wu,&nbsp;Zhenyu Li,&nbsp;Wentao Hong,&nbsp;Mengxi Cao,&nbsp;Xinhua Chen,&nbsp;Xueping Zhong,&nbsp;Qingchun Zhou,&nbsp;Haobin Zhao","doi":"10.1002/jez.2824","DOIUrl":"10.1002/jez.2824","url":null,"abstract":"<p>Methylosome protein 50 (Mep50) is a protein that is rich in WD40 domains, which mediate and regulate a variety of physiological processes in organisms. Previous studies indicated the necessity of Mep50 in embryogenesis in mice <i>Mus musculus</i> and fish. This study aimed to further understand the roles of maternal Mep50 in early embryogenesis using medaka <i>Oryzias latipes</i> as a model. Without maternal Mep50, medaka zygotes developed to the pre-early gastrula stage but died later. The transcriptome of the embryos at the pre-early gastrula stage was analyzed by RNA sequencing. The results indicated that 1572 genes were significantly upregulated and 741 genes were significantly downregulated in the embryos without maternal Mep50. In the differentially expressed genes (DEGs), the DNA-binding proteins, such as histones and members of the small chromosome maintenance complex, were enriched. The major interfered regulatory networks in the embryos losing maternal Mep50 included DNA replication and cell cycle regulation, AP-1 transcription factors such as Jun and Fos, the Wnt pathway, RNA processing, and the extracellular matrix. Quantitative RT-PCR verified 16 DEGs, including <i>prmt5</i>, <i>H2A</i>, <i>cpsf</i>, <i>jun</i>, <i>mcm4</i>, <i>myc</i>, <i>p21</i>, <i>ccne2</i>, <i>cdk6</i>, and <i>col1</i>, among others. It was speculated that the absence of maternal Mep50 could potentially lead to errors in DNA replication and cell cycle arrest, ultimately resulting in cell apoptosis. This eventually resulted in the failure of gastrulation and embryonic death. The results indicate the importance of maternal Mep50 in early embryonic development, particularly in medaka fish.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 7","pages":"798-810"},"PeriodicalIF":1.9,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140668732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted and untargeted metabolomic profiles in wild rabbit does (Oryctolagus cuniculus) of different breeding states (pregnant and lactating)","authors":"Pablo Jesús Marín-García,&nbsp;Carlos Rouco,&nbsp;Lola Llobat,&nbsp;Torben Larsen,&nbsp;Mette Skou Hedemann","doi":"10.1002/jez.2818","DOIUrl":"10.1002/jez.2818","url":null,"abstract":"<p>Ecological nutrition aims to unravel the extensive web of nutritional links that drives animals in their interactions with their ecological environments. Nutrition plays a key role in the success of European wild rabbit (<i>Oryctolagus cuniculus</i>) and could be affected by the breeding status of the animals and reflected in the metabolome of this species. As nutritional needs are considerably increased during pregnancy and lactation, the main objective of this work was to determine how the breeding status (pregnant and lactating) of European wild rabbit does affects nutritional requirements and their metabolome (using targeted and untargeted metabolomics), aiming to find a useful biomarker of breeding status and for monitoring nutritional requirements. To address this gap, 60 wild European rabbits were studied. Animals were divided according to their breeding status and only pregnant (n = 18) and lactating (n = 11) rabbit does were used (n = 29 in total). The body weight and length of each animal were analyzed. The relative and absolute chemical composition of the gastric content and whole blood sample were taken, and targeted and untargeted metabolomics were analyzed. As a main result, there were no differences in biometric measurements, gastric content, and targeted metabolomics, except for live weight and nonesterified fatty acids (NEFA), as pregnant animals showed higher live weight (+12%; <i>p</i> = 0.0234) and lower NEFA acid levels (−46%; <i>p</i> = 0.0262) than lactating females. Regarding untargeted metabolomics, a good differentiation of the metabolome of the two breeding groups was confirmed, and it was proven that pregnant animals showed higher plasmatic levels of succinic anhydride (3.48 more times; <i>p</i> = 0.0236), succinic acid (succinate) (3.1 more times; <i>p</i> = 0.0068) and propionic acid (3.98 more times; <i>p</i> = 0.0121) than lactating animals. However, lactating animals showed higher levels of N-[(3a,5b,7b)-7-hydroxy-24-oxo-3-(sulfoxide) cholan-24-yl]-Glycine (cholestadien) (2.4 more times; <i>p</i> &lt; 0.0420), 4-maleyl-acetoacetate (MAA) (3.2 more times; <i>p</i> &lt; 0.0364) and irilone (2.2 more times; <i>p</i> = 0.0451) than pregnant animals, any of these metabolites could be used as a potential biomarker. From these results, it can be concluded that the most notable changes were observed in the metabolome of individuals, with most of the changes observed being due to energy and protein mobilisation.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 7","pages":"743-752"},"PeriodicalIF":1.9,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jez.2818","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140670644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organization of the blood system of rhynchonellid brachiopod Hemithiris psittacea (Brachiopoda: Rhynchonelliformea)","authors":"Tatyana Kuzmina,&nbsp;Vladislav Kuzmin,&nbsp;Ekaterina Bogomolova,&nbsp;Vladimir Malakhov","doi":"10.1002/jez.2820","DOIUrl":"10.1002/jez.2820","url":null,"abstract":"<p>The brachiopods are sessile invertebrates with an unusual blood system, which consists of a long-branched dorsal vessel. It is still unknown how blood circulates in this system. In the present study, for the first time we propose the circulation of blood in brachiopod <i>Hemithiris psittacea</i> based on morphological and experimental data. The main heart is located on the dorsal side of the stomach and divides the dorsal vessel into anterior and posterior parts. The anterior part enters the lophophore, where it gives off blind branches to each tentacle. The posterior part passes by the funnels of the nephridia and forms a blindly closed network in the gonads. We suggest that the circulation of blood includes three successive stages. During the first phase of systole of the main heart, blood flows through the anterior dorsal vessel. During the second phase of systole, blood flows through the posterior dorsal vessel. During diastole, blood flows from the anterior and posterior vessels and fills the main heart. The origin of a peculiar blood system in brachiopods can be explained by reduction of the ventral vessel, which is probably correlates with the reduction of the ventral side of the brachiopod ancestor's body. Another peculiarity of brachiopod blood system is the presence of an ampullar heart, which functions as a blood depot and allows blood to move in the vessels in two directions in an oscillatory mode. The brachiopod blood system contains vessels lacking true endothelium and can be classified as an “incompletely closed” type.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 7","pages":"766-782"},"PeriodicalIF":1.9,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140670597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complexity of trophic relationships between Gammarus aequicauda (Amphipoda) and Eucypris mareotica (Ostracoda) in a hypersaline lake","authors":"Nickolai Shadrin,&nbsp;Vladimir Yakovenko,&nbsp;Elena Anufriieva","doi":"10.1002/jez.2821","DOIUrl":"10.1002/jez.2821","url":null,"abstract":"<p>In the hypersaline lakes of Crimea, only 5–7 species of animals are common including <i>Gammarus aequicauda</i> (Amphipoda) and <i>Eucypris mareotica</i> (Ostracoda). Two experiments were carried out to characterize the trophic interactions between these species. In one, <i>G. aequicauda</i> were used freshly caught from the lake, and in the other, after they were starved for 2.5 days in the laboratory. The experimental options were one male or female <i>G. aequicauda</i> and either 10, 20, or 30 ostracods. The gammarids actively fed on the ostracods, and significant differences were between females and males. The feeding intensity of males was higher than that of females. However, ostracods are not passive prey, they can actively attack their predator and are capable of causing them harm. Ostracods attacked a Gammarus only if it had captured one of the ostracods. Sex differences in <i>G. aequicauda</i> were also evident in the reaction to the group's aggressive behavior of ostracods. Interactions between Ostracoda and Gammarids cannot be considered unidirectional; they both may be prey or predators depending on the situation.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 6","pages":"717-726"},"PeriodicalIF":2.8,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140630870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drosophila melanogaster as a model organism for diabetes II treatment by the ethyl acetate fraction of Atriplex halimus L.","authors":"Omnia Montaser,&nbsp;Mona El-Aasr,&nbsp;Haytham O. Tawfik,&nbsp;Wesam S. Meshrif,&nbsp;Hanaa Elbrense","doi":"10.1002/jez.2812","DOIUrl":"10.1002/jez.2812","url":null,"abstract":"<p>Type 2 diabetes (T2D) is the most common metabolic disorder. The undesirable effects of synthetic drugs demand a search for safe antidiabetic agents. This study aimed to assess the antidiabetic activity of different fractions of <i>Atriplex halimus</i> (petroleum ether 60–80, methylene chloride, ethyl acetate, and <i>n</i>-butanol) using <i>Drosophila melanogaster</i> larvae. Titers of total glucose and trehalose, as well as larval weight, were measured and compared with those of control and diabetic larvae. The expression of <i>Drosophila</i> insulin-like peptides (DILP2 and DILP3) and adipokinetic hormone (AKH) was evaluated. The results revealed a significant increase in total glucose, trehalose, and a decrease in body weight in the larvae fed a high-sugar diet compared with those in the control. When larvae fed diets containing the tested fractions, the total glucose and trehalose decreased to the control level, and the body weight increased. DILP2, DILP3, and AKH exhibited significant decreases upon treatment with <i>A. halimus</i> ethyl acetate. Metabolomic profiling of the ethyl acetate fraction of <i>A. halimus</i> revealed the presence of flavonoids and flavonoid glycosides. After docking screening to predict the most powerful moiety, we discovered that flavonoid glycosides (especially eriodictyol-7-<i>O</i>-neohesperidoside) have a greater affinity for the pocket than the other moieties. The results indicated the therapeutic activity of the <i>A. halimus</i> ethyl acetate fraction against induced T2D in <i>Drosophila</i> larvae. The antidiabetic activity may be attributed to flavonoids, which are the main components of the <i>A. halimus</i> ethyl acetate fraction.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"341 6","pages":"702-716"},"PeriodicalIF":2.8,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140578283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}