Journal of experimental zoology. Part A, Ecological and integrative physiology最新文献_第3页

The potential ameliorative role of Dimercaptosuccinic acid against the toxicity of Titanium Dioxide Nanoparticles on Caelatura nilotica clams 二巯基丁二酸对二氧化钛纳米颗粒对蛤蜊毒性的潜在改善作用

IF 1.9 3区生物学

Journal of experimental zoology. Part A, Ecological and integrative physiology Pub Date : 2024-07-21 DOI: 10.1002/jez.2848

Hoda H. Abdel‑Azeem, Azza H. Mohamed, Gamalat Y. Osman, AbdElhafez R. AbdElhafez, Sherin K. Sheir

{"title":"The potential ameliorative role of Dimercaptosuccinic acid against the toxicity of Titanium Dioxide Nanoparticles on Caelatura nilotica clams","authors":"Hoda H. Abdel‑Azeem, Azza H. Mohamed, Gamalat Y. Osman, AbdElhafez R. AbdElhafez, Sherin K. Sheir","doi":"10.1002/jez.2848","DOIUrl":"10.1002/jez.2848","url":null,"abstract":"The prevalent use of nanoparticles has adverse negative effects on biosystems. Subsequently, this study aimed to use Caelatura nilotica to assess the ecotoxicity of TiO2 NPs and how Dimercaptosuccinic acid (DMSA) improves these effects. Two concentrations of TiO2 NPs (25 and 150 µg/L) were used for 28 days. TiO2 NPs bioaccumulation, gonadal weight, gonado-somatic index, and histopathological alterations of gonads were determined. The tissues’ accumulation of TiO2 NPs was concentration-time-dependent: it was 78.5 ± 28.93 μg/g dry weight in the exposed clams to 150 µg/L TiO2 NPs after 4 weeks of exposure. The gonadal weight and gonado-somatic index significantly decreased of the exposed group to 150 µg/L TiO2 NPs over the experimental period that they ended with values (1.01 ± 0.57 gm, 19.15 ± 7.75%, respectively). There are some histological alterations in the gonads of C. nilotica such as necrosis, deteriorated connective tissue, increased fibrous tissue, a reduced presence of mature sperms and mature ova, and irregular shapes of testicular/ovarian follicles. When using Dimercaptosuccinic acid (DMSA), this led to a reduction in accumulation of TiO2 NPs by the end of the experiment. So, C. nilotica is a promising model to reflect the adverse nano-toxics. DMSA emerges as a potentially valuable chelating agent that abolishes the negative effects of these nanoparticles.","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141734325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early life thermal conditioning alters heat-shock protein expression in response to an adult thermal stressor 生命早期的热调节会改变热休克蛋白的表达，以应对成年热应力。

IF 1.9 3区生物学

Journal of experimental zoology. Part A, Ecological and integrative physiology Pub Date : 2024-07-15 DOI: 10.1002/jez.2858

Alexander J. Hoffman, John W. Finger Jr., Andreas N. Kavazis, Haruka Wada

{"title":"Early life thermal conditioning alters heat-shock protein expression in response to an adult thermal stressor","authors":"Alexander J. Hoffman, John W. Finger Jr., Andreas N. Kavazis, Haruka Wada","doi":"10.1002/jez.2858","DOIUrl":"10.1002/jez.2858","url":null,"abstract":"Developmental environmental stressors can have instructive effects on an organism's phenotype. This developmental plasticity can prepare organisms for potentially stressful future environments, circumventing detrimental effects on fitness. However, the physiological mechanisms underlying such adaptive plasticity are understudied, especially in vertebrates. We hypothesized that captive male zebra finches (Taeniopygia castanotis) exposed to a mild heat conditioning during development would acquire a persisting thermotolerance, and exhibit increased heat-shock protein (HSP) levels associated with a decrease in oxidative damage when exposed to a high-intensity stressor in adulthood. To test this, we exposed male finches to a prolonged mild heat conditioning (38°C) or control (22°C) treatment as juveniles. Then in a 2 × 2 factorial manner, these finches were exposed to a high heat stressor (42°C) or control (22°C) treatment as adults. Following the adult treatment, we collected testes and liver tissue and measured HSP70, HSP90, and HSP60 protein levels. In the testes, finches exhibited lower levels of HSP90 and HSP60 when exposed to the high heat stressor in adulthood if they were exposed to the mild heat conditioning as juveniles. In the liver, finches exposed to the high heat stressor in adulthood had reduced HSP90 and HSP60 levels, regardless of whether they were conditioned as juveniles. In some cases, elevated testes HSP60 levels were associated with increased liver oxidative damage and diminishment of a condition-dependent trait, indicating potential stress-induced tradeoffs. Our results indicate that a mild conditioning during development can have persisting effects on HSP expression and acquired thermotolerance.","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141616554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A single gene determines allorecognition in hydrozoan jellyfish Cladonema radiatum inbred lines 单个基因决定了水螅水母雷蒂藻（Cladonema radiatum）近交系的异源识别能力。

IF 1.9 3区生物学

Journal of experimental zoology. Part A, Ecological and integrative physiology Pub Date : 2024-07-08 DOI: 10.1002/jez.2853

Crystal Tang, Miwa Tamura-Nakano, Kenta Kobayakawa, Takuto Ozawa, Takao Onojima, Rei Kajitani, Takehiko Itoh, Kazunori Tachibana

{"title":"A single gene determines allorecognition in hydrozoan jellyfish Cladonema radiatum inbred lines","authors":"Crystal Tang, Miwa Tamura-Nakano, Kenta Kobayakawa, Takuto Ozawa, Takao Onojima, Rei Kajitani, Takehiko Itoh, Kazunori Tachibana","doi":"10.1002/jez.2853","DOIUrl":"10.1002/jez.2853","url":null,"abstract":"Allorecognition—the ability of an organism to discriminate between self and nonself—is crucial to colonial marine animals to avoid invasion by other individuals in the same habitat. The cnidarian hydroid Hydractinia has long been a major research model in studying invertebrate allorecognition, establishing a rich knowledge foundation. In this study, we introduce a new cnidarian model Cladonema radiatum (C. radiatum). C. radiatum is a hydroid jellyfish which also forms polyp colonies interconnected with stolons. Allorecognition responses—fusion or regression of stolons—are observed when stolons encounter each other. By transmission electron microscopy, we observe rapid tissue remodeling contributing to gastrovascular system connection in fusion. Meanwhile, rejection responses are regulated by reconstruction of the chitinous exoskeleton perisarc, and induction of necrotic and autophagic cellular responses at cells in contact with the opponent. Genetic analysis identifies allorecognition genes: six Alr genes located on the putative allorecognition complex and four immunoglobulin superfamily genes on a separate genome region. C. radiatum allorecognition genes show notable conservation with the Hydractinia Alr family. Remarkedly, stolon encounter assays of inbred lines reveal that genotypes of Alr1 solely determine allorecognition outcomes in C. radiatum.","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jez.2853","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141554872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic effect of Momordica charantia on cardiomyopathy in a diabetic maternal rat model 论文标题：毛果芸香碱对糖尿病母鼠心肌病模型的治疗作用

IF 1.9 3区生物学

Journal of experimental zoology. Part A, Ecological and integrative physiology Pub Date : 2024-07-08 DOI: 10.1002/jez.2854

Shaimaa M. Elnahas, Hend Abd El-Halim Mansour, Mamdouh R. El-Sawi, Amoura M. Abou-El-Naga

{"title":"Therapeutic effect of Momordica charantia on cardiomyopathy in a diabetic maternal rat model","authors":"Shaimaa M. Elnahas, Hend Abd El-Halim Mansour, Mamdouh R. El-Sawi, Amoura M. Abou-El-Naga","doi":"10.1002/jez.2854","DOIUrl":"10.1002/jez.2854","url":null,"abstract":"Myocardial structural and functional abnormalities are hallmarks of diabetic cardiomyopathy (DCM), a chronic consequence of diabetes mellitus (DM). Maternal DM affects and increases the risk of heart defects in diabetic mothers compared with nondiabetic mothers. Momordica charantia exhibits antidiabetic effects due to various bioactive compounds that are phytochemicals, a broad group that includes phenolic compounds, alkaloids, proteins, steroids, inorganic compounds, and lipids. Pregnant maternal rats were split into four groups: control (C), M. charantia-treated (MC), type 2 diabetes mellitus (T2DM) (DM), and diabetic (MC + DM) groups. Diabetes mothers had increased serum glucose, insulin, total cholesterol, triglyceride, and low-density lipoprotein cholesterol levels and reduced high-density lipoprotein cholesterol levels. Cardiac biomarkers such as cardiac troponin T (cTnT), creatine kinase-myocardial band (CK-MB), and lactate dehydrogenase were increased. Hormone levels of follicle-stimulating hormone, luteinizing hormone, progesterone, and estrogen decreased significantly. Inflammatory markers such as interleukin 6 (IL-6), tumor necrosis factor-alpha (TNF-α), and vascular adhesion molecule-1 (VCAM-1) were elevated in diabetic mothers. Oxidative stress markers indicated increased malondialdehyde and nitric oxide levels, while antioxidants such as glutathione, superoxide dismutase, and catalase were decreased in maternal heart tissue. The levels of apoptotic markers such as tumor suppressor 53 (P53) and cysteine aspartic protease-3 (caspase-3) were significantly greater in diabetic maternal heart tissue. Histopathological analysis revealed heart tissue abnormalities in diabetic maternal rats. M. charantia extract improved maternal diabetes-induced changes in inflammation, antioxidant levels, and heart tissue structure.","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141554899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protocol to record and analyze primate leaping in three dimensions in the wild 记录和分析野生灵长类动物三维跳跃的程序。

IF 1.9 3区生物学

Journal of experimental zoology. Part A, Ecological and integrative physiology Pub Date : 2024-07-08 DOI: 10.1002/jez.2849

Judith Janisch, Jack Kirven, Nicole Schapker, Lydia C. Myers, Liza J. Shapiro, Jesse W. Young

{"title":"Protocol to record and analyze primate leaping in three dimensions in the wild","authors":"Judith Janisch, Jack Kirven, Nicole Schapker, Lydia C. Myers, Liza J. Shapiro, Jesse W. Young","doi":"10.1002/jez.2849","DOIUrl":"10.1002/jez.2849","url":null,"abstract":"Several studies comparing primate locomotion under lab versus field conditions have shown the importance of implementing both types of studies, as each has their advantages and disadvantages. However, three-dimensional (3D) motion capture of primates has been challenging under natural conditions. In this study, we provide a detailed protocol on how to collect 3D biomechanical data on primate leaping in their natural habitat that can be widely implemented. To record primate locomotion in the dense forest we use modified GoPro Hero Black cameras with zoom lenses that can easily be carried around and set up on tripods. We outline details on how to obtain camera calibrations at greater heights and how to process the collected data using the MATLAB camera calibration app and the motion tracking software DLTdv8a. We further developed a new MATLAB application “WildLeap3D” to generate biomechanical performance metrics from the derived x, y, z coordinates of the leaps. We provide details on how to collect data on support diameter, compliance, and orientation, and combine these with the jumps to study locomotor performance in an ecological context. We successfully reconstructed leaps of wild primates in the 3D space under natural conditions and provided data on four representative leaps. We provide exemplar data on primate velocity and acceleration during a leap and show how our protocol can be used to analyze segmental kinematics. This study will help to make motion capture of freely moving animals more accessible and help further our knowledge about animal locomotion and movement.","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jez.2849","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141554900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toxocara canis: Prospective activity of Quercetin and venom of Cassiopea andromeda (Cnidaria: Cassiopeidae) against third-stage larvae in vitro 犬弓形虫：槲皮素和 Cassiopea andromeda（Cnidaria: Cassiopeidae）毒液对体外第三期幼虫的前瞻性活性。

IF 1.9 3区生物学

Journal of experimental zoology. Part A, Ecological and integrative physiology Pub Date : 2024-07-08 DOI: 10.1002/jez.2852

Rasha A. Elmahy, Alaa Y. Moustafa, Nahla A. Radwan

{"title":"Toxocara canis: Prospective activity of Quercetin and venom of Cassiopea andromeda (Cnidaria: Cassiopeidae) against third-stage larvae in vitro","authors":"Rasha A. Elmahy, Alaa Y. Moustafa, Nahla A. Radwan","doi":"10.1002/jez.2852","DOIUrl":"10.1002/jez.2852","url":null,"abstract":"Toxocariasis is a zoonotic parasitic infection with worldwide distribution and high impact on human health. It has a limited clinical resolution with the available drugs, making it challenging to treat. Quercetin, which possesses biological and pharmacological qualities including antiparasitic, antioxidant, and anticancer activities, is a possible substitute for the current medications. Marine invertebrates can produce a vast array of different molecules, many of which are biologically active substances with distinct characteristics. In this study, we assessed the in vitro nematocidal effect of both quercetin and venom of Cassiopea andromeda (jellyfish) against third larvae of Toxocara canis. In microplates with Roswell Park Memorial Institute-1640 medium, larvae were incubated with ethanolic extract of quercetin (0.01, 0.02, 0.05, 0.08, 0.1, 0.25, and 0.5 mM/mL) and water extract of C. andromeda venom (15, 20, 25, 30, 35, 40, and 60 µg/mL) to evaluate their larvicidal effect. A scanning electron microscopy has investigated the possible effect of lethal concentration (LC90) of both extracts on the body wall of cultivated larvae, in comparison with those cultivated in albendazole. Our study revealed the effects of both quercetin and C. andromeda venom exposure on the mortality rate and the ultrastructure of T. canis third larva in comparison with control and albendazole-treated groups.","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141554901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hyperandrogenemia elevates expression of apelin and apelin receptor protein in the mice pituitary 高雄激素血症会提高小鼠垂体中 apelin 和 apelin 受体蛋白的表达。

IF 1.9 3区生物学

Journal of experimental zoology. Part A, Ecological and integrative physiology Pub Date : 2024-07-08 DOI: 10.1002/jez.2857

Preethi Riba, Borgohain Anima, Ayushmita Dutta, Guruswami Gurusubramanian, Vikas Kumar Roy

{"title":"Hyperandrogenemia elevates expression of apelin and apelin receptor protein in the mice pituitary","authors":"Preethi Riba, Borgohain Anima, Ayushmita Dutta, Guruswami Gurusubramanian, Vikas Kumar Roy","doi":"10.1002/jez.2857","DOIUrl":"10.1002/jez.2857","url":null,"abstract":"Hyperandrogenemia is associated with polycystic ovarian syndrome (PCOS) and imbalances in the pituitary hormones, luteinizing hormone (LH) and follicle-stimulating hormone (FSH) levels. Apelin and its receptor, APJ (class A, rhodopsin-like G- protein-coupled receptor), belongs to adipokines, and its expression has been shown in the pituitary. It is also well known that, hyperandrogenism and PCOS have deregulation of different adipokines. Whether hyperandrogenism also deregulates the apelin system in the pituitary has yet to be investigated. Thus, we have investigated the expression and localization of apelin and its receptor, APJ, in the letrozole-induced hyperandrogenised pituitary of female mice. Our results showed that the apelin, APJ and androgen receptor (AR) expression were upregulated in the anterior pituitary. Furthermore, the immunostaining of LH exhibited increased abundance than FSH. The circulating LH was also found to be elevated compared to FSH levels. The increased LH synthesis and secretion coincides with elevated apelin system in the pituitary of hyperandrogenised mice. Recently, a direct role of apelin has also been reported in the female pituitary, where apelin inhibits LH secretion. Thus, apelin could be one of the factors for deregulated gonadotropin secretion in hyperandrogenised conditions. However, more research is needed to fully understand the complex interactions between apelin and androgen regarding gonadotropin secretion in hyperandrogenised conditions.","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141554898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploration of gut microbial diversity of pheasants through pyrosequencing of 16S rRNA gene 通过 16S rRNA 基因热释光测序探索雉鸡肠道微生物的多样性。

IF 1.9 3区生物学

Journal of experimental zoology. Part A, Ecological and integrative physiology Pub Date : 2024-07-05 DOI: 10.1002/jez.2855

Syed M. Bukhari, Shahla Andleeb, Huda A. Alghamdi, Khalil ur Rehman, Arshad Javid, Waqas Ali, Yassar Abbas, Kashaf Nokhaiz

{"title":"Exploration of gut microbial diversity of pheasants through pyrosequencing of 16S rRNA gene","authors":"Syed M. Bukhari, Shahla Andleeb, Huda A. Alghamdi, Khalil ur Rehman, Arshad Javid, Waqas Ali, Yassar Abbas, Kashaf Nokhaiz","doi":"10.1002/jez.2855","DOIUrl":"10.1002/jez.2855","url":null,"abstract":"Despite the diversity of microbiota in birds is similar to that of other animals, there is a lack of research on the gut microbial diversity of nondomesticated bird species. This study aims to address this gap in knowledge by analyzing the bacterial communities present in the gut of two important game bird species, the Ring-necked pheasant (Phasianus colchicus) and the Green pheasant (Phasianus versicolor) to understand the gut microbial diversity of these species. The gut microbiome of 10 individual pheasants from two different species was studied using pooled fecal samples. We used 16S rRNA gene sequencing on the Ion S5 XL System next-generation sequencing with Mothur and SILVA Database for taxonomic division. An average of 141 different operational taxonomic units were detected in the gut microbiome. Analysis of microbial classification revealed the presence of 191 genera belonging to 12 different phyla in both pheasants. Alpha diversity indices revealed that P. colchicus exhibited most prevalence firmicutes with bacillus species microbial community than P. versicolor. Alpha diversity indices indicated that P. colchicus had a more diverse community and P. versicolor had a greater diversity of evolutionary lineages, while both species had similar levels of species richness and sample inclusiveness. These findings may have implications for the health and well-being of pheasants, serving as a reference for their bacterial diversity. Additionally, they provide a baseline for future research and conservation efforts aimed at improving the health and well-being of these and possibly other avian species.","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141534489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of acute hypoxia exposure and acclimation on the thermal tolerance of an imperiled Canadian minnow 急性缺氧暴露和适应对加拿大濒危小鱼热耐受性的影响。

IF 1.9 3区生物学

Journal of experimental zoology. Part A, Ecological and integrative physiology Pub Date : 2024-07-05 DOI: 10.1002/jez.2847

Jessica E. Reemeyer, Lauren J. Chapman

{"title":"Effects of acute hypoxia exposure and acclimation on the thermal tolerance of an imperiled Canadian minnow","authors":"Jessica E. Reemeyer, Lauren J. Chapman","doi":"10.1002/jez.2847","DOIUrl":"10.1002/jez.2847","url":null,"abstract":"Elevated water temperatures and low dissolved oxygen (hypoxia) are pervasive stressors in aquatic systems that can be exacerbated by climate change and anthropogenic activities, and there is growing interest in their interactive effects. To explore this interaction, we quantified the effects of acute and long-term hypoxia exposure on the critical thermal maximum (CTmax) of Redside Dace (Clinostomus elongatus), a small-bodied freshwater minnow with sparse populations in the Great Lakes Basin of Canada and designated as Endangered under Canada's Species at Risk Act. Fish were held at 18°C and acclimated to four levels of dissolved oxygen (>90%, 60%, 40%, and 20% air saturation). CTmax was measured after 2 and 10 weeks of acclimation and after 3.5 weeks of reoxygenation, and agitation behavior was quantified during CTmax trials. Aquatic surface respiration behavior was also quantified at 14 weeks of acclimation to oxygen treatments. Acute hypoxia exposure decreased CTmax in fish acclimated to normoxia (>90% air saturation), but acclimation to hypoxia reduced this effect. There was no effect of acclimation oxygen level on CTmax when measured in normoxia, and there was no effect of exposure time to hypoxia on CTmax. Residual effects of hypoxia acclimation on CTmax were not seen after reoxygenation. Agitation behavior varied greatly among individuals and was not affected by oxygen conditions. Fish performed aquatic surface respiration with low frequency, but performed it earlier when acclimated to higher levels of oxygen. Overall, this work sheds light on the vulnerability of fish experiencing acute hypoxia and heat waves concurrently.","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jez.2847","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141534570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An electrophysiological correlate of sleep in a shark. 鲨鱼睡眠的电生理相关性。

IF 1.9 3区生物学

Journal of experimental zoology. Part A, Ecological and integrative physiology Pub Date : 2024-07-03 DOI: 10.1002/jez.2846

John A Lesku, Paul-Antoine Libourel, Michael L Kelly, Jan M Hemmi, Caroline C Kerr, Shaun P Collin, Craig A Radford

{"title":"An electrophysiological correlate of sleep in a shark.","authors":"John A Lesku, Paul-Antoine Libourel, Michael L Kelly, Jan M Hemmi, Caroline C Kerr, Shaun P Collin, Craig A Radford","doi":"10.1002/jez.2846","DOIUrl":"https://doi.org/10.1002/jez.2846","url":null,"abstract":"Sleep is a prominent physiological state observed across the animal kingdom. Yet, for some animals, our ability to identify sleep can be masked by behaviors otherwise associated with being awake, such as for some sharks that must swim continuously to push oxygenated seawater over their gills to breathe. We know that sleep in buccal pumping sharks with clear rest/activity cycles, such as draughtsboard sharks (Cephaloscyllium isabellum, Bonnaterre, 1788), manifests as a behavioral shutdown, postural relaxation, reduced responsiveness, and a lowered metabolic rate. However, these features of sleep do not lend themselves well to animals that swim nonstop. In addition to video and accelerometry recordings, we tried to explore the electrophysiological correlates of sleep in draughtsboard sharks using electroencephalography (EEG), electromyography, and electrooculography, while monitoring brain temperature. The seven channels of EEG activity had a surprising level of (apparent) instability when animals were swimming, but also when sleeping. The amount of stable EEG signals was too low for replication within- and across individuals. Eye movements were not measurable, owing to instability of the reference electrode. Based on an established behavioral characterization of sleep in draughtsboard sharks, we offer the original finding that muscle tone was strongest during active wakefulness, lower in quietly awake sharks, and lowest in sleeping sharks. We also offer several critical suggestions on how to improve techniques for characterizing sleep electrophysiology in future studies on elasmobranchs, particularly for those that swim continuously. Ultimately, these approaches will provide important insights into the evolutionary confluence of behaviors typically associated with wakefulness and sleep.","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141492147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0