Journal of experimental zoology. Part A, Ecological and integrative physiology最新文献

{"title":"Association Between Back Scent Gland Development and Reproductive Status in Male Brown Bears (Ursus arctos)","authors":"Jumpei Tomiyasu,&nbsp;Naoya Matsumoto,&nbsp;Hinako Katsushima,&nbsp;Asuka Nishijima,&nbsp;Kyogo Hagino,&nbsp;Hideyuki Sakamoto,&nbsp;Yojiro Yanagawa","doi":"10.1002/jez.2916","DOIUrl":"10.1002/jez.2916","url":null,"abstract":"<div>\u0000 \u0000 <p>Cutaneous scent glands secrete chemical signals for communication, a significant portion of which is to convey information about male reproductive status. This might be particularly important to animals with intraspecific variation in male reproductive activity, such as bears. Male brown bears mark trees with their scent secreted from back apocrine and sebaceous glands during the breeding season and thus might convey information about reproductive status. Therefore, we speculated that the development of scent glands in the back reflects reproductive performance. We investigated the effects of reproductive status on the development of scent glands in the back using contraception and explored relationships between reproductive parameters (testosterone levels, testis size, and spermatogenic scores) and the size of skin glands in the backs of intact male brown bears. During the breeding season, we sampled back and rump (control) skin, sperms by urethral catheterization, and blood under anesthesia from four male bears that were vaccinated with the contraceptive gonadotropin hormone-releasing hormone (GnRH) vaccine and five others that were not (controls). The back sebaceous and apocrine glands were significantly smaller in bears vaccinated with than without GnRH vaccine, suggesting that these glands are associated with reproductive status. Testosterone levels were significantly and positively associated with the size of sebaceous glands, whereas none of the reproductive parameters were associated with the size of either sebaceous or apocrine glands. Mating opportunities increase testosterone production. The positive association between testosterone and sebaceous glands might mean that sebaceous glands in the back can secrete chemicals on demand for reproduction.</p></div>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 5","pages":"629-635"},"PeriodicalIF":1.9,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corticosterone and Mitochondrial Efficiency Are Associated With Changes in DNA Oxidative Damage During an Acute Stress Response in Leach's Storm-Petrels (Hydrobates leucorhous).","authors":"Kayla E Lichtner, Jack K Dziubek, Nicole A Joseph, Sarah E Chapman, Tori J Chace, Dongxiao Sun, Zachary T Bitzer, Antoine Stier, Robert A Mauck, Patricia L Jones, Mark F Haussmann","doi":"10.1002/jez.2917","DOIUrl":"https://doi.org/10.1002/jez.2917","url":null,"abstract":"<p><p>The ability of organisms to effectively respond to challenges is critical for survival. We investigated how an acute stressor affected corticosterone, mitochondrial function, and DNA oxidative damage in a wild population of Leach's storm-petrels (Hydrobates leucorhous). We conducted a standardized 20-min handling procedure on storm-petrel chicks and collected baseline and post-handling blood samples. We measured plasma corticosterone and red blood cell DNA oxidative damage levels through the detection of a mutated DNA base 8-Hydroxy-2'-deoxyguanosine (8-OHdG). In addition, we quantified six measures of mitochondrial aerobic metabolism from red blood cells. Overall, the handling stressor increased plasma corticosterone levels and decreased mitochondrial efficiency to produce ATP. Although the increase in corticosterone was inversely related to the change in DNA oxidative damage, the decrease in mitochondrial efficiency was positively correlated with the change in DNA oxidative damage. Thus, over an acute stress response, individuals who had the largest increase in corticosterone also had the least amount of oxidative damage. In addition, individuals who prioritized ATP production during the acute stress also showed higher levels of oxidative damage. This work highlights the complex pathways by which corticosterone and mitochondrial efficiency affect oxidative damage during acute stress, providing new insights into the trade-offs underlying physiological responses in wild animals.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interactive Effects of Temperature Acclimation and Dietary Fatty Acids on Metabolic Rate and Body Composition of Zebra Finches (Taeniopygia guttata)","authors":"Michael J. Campbell,&nbsp;Gabriela F. Mastromonaco,&nbsp;Gary Burness","doi":"10.1002/jez.2915","DOIUrl":"10.1002/jez.2915","url":null,"abstract":"<p>Climate change is contributing to geographic range shifts in many bird species, with possible exposure to novel diets. How individuals respond physiologically across chronic time frames to the interacting effects of diet and environmental temperature has been little explored. We acclimated zebra finches to either cool (20°C–24°C) or thermoneutral (35°C) temperatures over 6 months and provided them with diets enriched in either unsaturated or saturated fatty acids. We measured body mass throughout the study, and basal metabolic rate (BMR) and body composition at 3 and 6 months, respectively. Individuals held in cool conditions and fed a diet enriched with unsaturated fatty acids lost mass relative to the other groups, and after 6 months were of similar mass to individuals maintained at thermoneutrality. Chronic exposure to cool conditions increased BMR and the mass of the pectoral muscle and visceral organs. However, we could detect no long-term effect of diet on any physiological parameter. Our results contrast with those of birds studied over acute time frames, in which diet and temperature interact to determine energy expenditure. Over chronic time frames individuals appear to reach a new steady-state, with long-term physiological responses driven primarily by thermoregulatory responses to environmental temperature.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 5","pages":"617-628"},"PeriodicalIF":1.9,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jez.2915","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Longitudinal Changes in Aerodigestive Responses to Varying Nipple Properties in Infant Pigs","authors":"François D. H. Gould,&nbsp;Christopher Mayerl,&nbsp;Khaled Adjerid,&nbsp;Jessica Reibel,&nbsp;Nichole Charles,&nbsp;Chloe Edmonds,&nbsp;Rebecca Z. German","doi":"10.1002/jez.2914","DOIUrl":"10.1002/jez.2914","url":null,"abstract":"<p>Coordination of respiration and swallowing is critical for survival in infant mammals and changes throughout infant maturation. Infants show sensorimotor feeding responses to changes in physical properties of nipples. Commercial nipples are promoted as improving aerodigestive coordination. However, it is unknown how nipple properties influence coordination of respiration and swallowing. Furthermore, how infant-nipple interactions change throughout maturation is not well understood. We designed and manufactured four silicone nipples of the same shape that varied in two parameters: nipple opening diameter and nipple stiffness. We used our validated infant pig model to test the impact of nipple parameters on aerodigestive coordination throughout ontogeny. We recorded videofluoroscopy and simultaneous chest plethysmography on six infant pigs at age 7 and 17 days when feeding on each of the four nipples presented in a randomized order. We measured the time between a swallow and the closest inspiration, and the length of the respiratory cycles during feeding. Larger nipple opening diameter resulted in shorter delay between swallow and onset of inspiration regardless of stiffness or pig age. In young pigs, respiratory cycle length was shorter when feeding on nipples with large diameter openings. In older pigs, there was a nipple-stiffness interaction such that respiratory cycle length increased with opening diameter in soft nipples but decreased with opening diameter in hard nipples. Nipple opening diameter has a pervasive effect on swallow-breathing coordination in freely feeding infants, but infant maturation modifies responses to nipple properties.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 5","pages":"608-616"},"PeriodicalIF":1.9,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jez.2914","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"L-Citrulline Alleviates Testicular Ischemia/Reperfusion Injury in Rats by Modulating eNOS/iNOS Induced Nitric Oxide Production, Inflammation, and Apoptosis","authors":"Eman M. Embaby,&nbsp;Aya Megahed,&nbsp;Sally Abdallah Mostafa,&nbsp;Alaa Samy,&nbsp;Eman H. Yousef,&nbsp;Amal F. Dawood,&nbsp;Mamdouh Eldesoqui","doi":"10.1002/jez.2913","DOIUrl":"10.1002/jez.2913","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 <p>Testicular ischemia/reperfusion injury (TI/RI) is a significant clinical contributor to subfertility and infertility resulting from testicular torsion and subsequent detortion. Insufficient nitric oxide (NO) synthesis in TI/RI can result in endothelial dysfunction, as the vascular endothelium fails to produce sufficient NO to sustain appropriate vasodilation and blood perfusion. Many studies have found that NO plays an important role in the I/RI and its increase or decrease can affect the progression and outcome of I/RI. However, the role of NO in I/RI is controversial and complicated. NO derived by endothelial NO synthase (eNOS) shows a protective role in I/RI, while excessive NO derived by inducible NO synthase (iNOS) accelerates inflammation and increases oxidative stress, further aggravating I/RI. Nevertheless, the overexpression of eNOS may exacerbate I/RI. Here we try to investigate the new progress in the understanding of the roles of NO during I/RI. This study examined the interplay between cytotoxic and cytoprotective mechanisms underpinning NO produced from L-citrulline (L-Cit) on TI/R injured rats. Thirty-two adult Sprague-Dawley albino rats were equally randomized into the following groups: normal control group, sham group, TI/R group (3 h/4 h), and TI/R + L-Cit group (600 mg/kg) orally at 1 h before reperfusion. Compared to the TI/R-operated group, the injection of L-Cit markedly enhanced serum concentrations of reproductive hormones (<i>p</i> &lt; 0.05). Elevated SOD, CAT, and GPx activity, along with reduced MDA and NO concentrations, indicated a diminished oxidative stress. The testicular levels of TNF-α, IL-1β, caspase-3, BAX, eNOS, iNOS, and NF-κB p65 were markedly reduced. Histopathological analysis corroborated the protective effect of L-Cit. The findings confirmed molecular models, demonstrating that L-Cit inhibited eNOS, iNOS, and IKKβ. The results showed that giving torsioned rats NO made from L-Cit protected them against hormonal imbalance, oxidative stress, inflammation, and apoptosis in I/RI. This makes L-Cit even more important for protecting against tissue I/RI during surgery. L-Cit not only promoted NO synthesis through eNOS activation, but it also facilitated the neutralization of iNOS production and its pathogenic NO levels during the reperfusion phase in I/R-injured rats.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 5","pages":"590-607"},"PeriodicalIF":1.9,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Respiratory Metabolism and Metabolomics of Red Swamp Crayfish Procambarus clarkii Under Low Temperature Stress","authors":"Yu Ding,&nbsp;Wenbin Sha,&nbsp;Yunfei Sun,&nbsp;Yongxu Cheng","doi":"10.1002/jez.2912","DOIUrl":"10.1002/jez.2912","url":null,"abstract":"<div>\u0000 \u0000 <p>In the integrated rice-crayfish aquaculture systems, crayfish <i>Procambarus clarkii</i> need to spend a long winter in the ring ditch, which is vulnerable to low temperature stress, especially in the northern part of China, where cold waves and other low-temperature climates are frequent. To study the metabolic response of <i>P. clarkii</i> to low temperature stress experimentally, the temperature was lowered from the control (23°C) to the low temperature group (9°C, 5°C, and 1°C) by slow and uniform cooling, and molecular and physiological samples were collected for measurement. The results showed that low temperature stress damaged the gill membrane and the epithelial layer of gill tissues, with an increase in vacuoles area and a reduced and irregular distribution of hemocytes. As the temperature decreased, the oxygen consumption rate, ammonia excretion rate and maximum metabolic rate of <i>P. clarkii</i> decreased gradually, the oxygen-nitrogen ratio decreased but still remained at a high level, and the metabolic energy supply substances were always mainly lipids and carbohydrates. The pyruvate kinase activity tended to increase with decreasing temperature under low temperature stress, while hexokinase, succinate dehydrogenase and lactate dehydrogenase activities decreased gradually. The 183 differential metabolites were screened in the low temperature group compared with the control mostly enriched in amino acid metabolism and citrate cycle metabolic pathways. In conclusion, under low temperature stress, the gill was damaged, respiratory metabolism decreased, and glycolysis was enhanced. Since the citrate cycle metabolism was suppressed, <i>P. clarkii</i> needed to resist low temperature stress by increasing the amino acid metabolism to provide more energy to maintain cellular activity. The results unraveled metabolic response mechanisms of metabolic response mechanism of <i>P. clarkii</i> to low temperature stress, and provided theoretical references for the selection and breeding of low-temperature-tolerant strains of <i>P. clarkii</i>.</p></div>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 5","pages":"578-589"},"PeriodicalIF":1.9,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-Dimensional Limb Kinematics in Brown-Throated Three-Toed Sloths (Bradypus variegatus) During Suspensory Quadrupedal Locomotion","authors":"Angela M. Mossor,&nbsp;Andrew J. McKamy,&nbsp;Melody W. Young,&nbsp;Andrew J. Rochté,&nbsp;Judy A. Avey-Arroyo,&nbsp;John A. Nyakatura,&nbsp;Michael C. Granatosky,&nbsp;Michael T. Butcher,&nbsp;Jesse W. Young","doi":"10.1002/jez.2911","DOIUrl":"10.1002/jez.2911","url":null,"abstract":"<p>Suspensory locomotion differs significantly from upright quadrupedal locomotion in mammals. Nevertheless, we know little concerning joint kinematics of suspensory movement. Here, we report three-dimensional kinematic data during locomotion in brown-throated three-toed sloths (<i>Bradypus variegatus</i>). Individuals were recorded with four calibrated high-speed cameras while performing below-branch locomotion on a simulated branch. The elbow (range 73°–177°; mean 114°) and knee (range 107°–175°; mean 140°) were extended throughout support phase, with elbow extension increasing with speed. Both the fore- and hindlimb displayed abducted proximal limb elements (i.e., arm and thigh) and adducted distal elements (i.e., forearm and leg) during all support phase points. Comparisons of elbow and knee angles between brown-throated three-toed sloths and Linnaeus's two-toed sloths (<i>Choloepus didactylus</i>) showed that brown-throated three-toed sloths had significantly more extended joint positions during all support phase points. Additionally, across all kinematic measurements, brown-throated three-toed sloths showed significant differences between homologous fore- and hindlimb segments, with the knee being more extended than the elbow and the arm being more abducted than the thigh. These results are consistent with previously established morphological and behavioral differences between extant sloth genera, with three-toed sloths showing significantly longer forelimbs than hindlimbs and typically favoring locomotion on angled supports. Our findings show that, despite overall similarities in the use of below-branch quadrupedal locomotion, the two sloth lineages achieve this locomotor mode with differing kinematic strategies (e.g., degree of joint flexion). These differences may be attributed to the distinct evolutionary pathways through which obligate suspensory locomotion arose in each lineage.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 5","pages":"564-577"},"PeriodicalIF":1.9,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jez.2911","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143541710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Responses to Hypoxia and Hyperoxia in Embryonic Tiger Keelbacks (Rhabdophis tigrinus lateralis; Colubridae)","authors":"Xiang-Mo Li,&nbsp;Shan Su,&nbsp;Lu-Wen Zhang,&nbsp;Yan-Qing Wu,&nbsp;Xiang Ji","doi":"10.1002/jez.2910","DOIUrl":"10.1002/jez.2910","url":null,"abstract":"<div>\u0000 \u0000 <p>Studies examining the oxygen dependency of embryonic survival, growth, and differentiation have been conducted for decades in a diverse array of animal taxa but including only one oviparous snake, the viperine water snake <i>Natrix maura</i>. Here, we describe a study incubating eggs of the tiger keelback <i>Rhabdophis tigrinus lateralis</i> (Colubridae) under four oxygen conditions, hypoxia (7% and 11% O₂), normoxia (21% O₂), and hyperoxia (31% O₂), for different lengths or at different stages of incubation. The length of hypoxic exposure is important in affecting embryonic development in <i>R. t. lateralis</i>, with prolonged hypoxic exposure retarding embryonic growth and differentiation, increasing embryonic mortality and deformity, reducing hatchling size and mass, and altering hatchling body shape relative to normoxic controls. Embryonic tiger keelbacks are most susceptible to hypoxia late in development, as revealed by the fact that a 5-day exposure of eggs to hypoxia of 7% O₂ reduced embryo mass and hatchling mass if it occurred at late stages of incubation. Hyperoxia of 31% O₂ did not enhance development of <i>R. t. lateralis</i> embryos, only affecting hatchling head width, which slightly differed between hyperoxic hatchlings and their normoxic siblings. This study demonstrates the importance of avoiding hypoxic exposure at late stages of embryonic development in snakes.</p></div>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 5","pages":"554-563"},"PeriodicalIF":1.9,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143390992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Food Restriction During Development Impairs Reproductive Success but not Ornamentation in Zebra Finches","authors":"Victoria M. Coutts,&nbsp;Kayci I. Messerly,&nbsp;Haruka Wada","doi":"10.1002/jez.2909","DOIUrl":"10.1002/jez.2909","url":null,"abstract":"<div>\u0000 \u0000 <p>Stress during development can elicit lifelong changes to an organism. However, whether these changes are beneficial or detrimental can only be determined by their effect on fitness outcomes. Furthermore, the effect of severe, chronic food restriction on the development of ornamentation, which can influence attractiveness, still needs to be explored. In this study, zebra finches (<i>Taeniopygia castanotis</i>) were exposed to either an <i>ad libitum</i> (control) diet or a 40% restricted (food-restricted) diet throughout postnatal development. Pictures of beaks and cheek patches were captured throughout the juvenile period and into adulthood to measure the development of secondary sexual characteristics. In adulthood, females were paired with nonexperimental males to measure reproductive success. There was no effect of treatment on beak color development or final beak color, but food-restricted males developed cheek patches slower and had more asymmetry during cheek patch development. However, food restriction did not impact the final cheek patch color or size. Food-restricted females were found to have lower reproductive success than control females. These results suggest that while cheek patch ornamentation can experience ‘catch-up growth’, and beak and cheek patch color do not reflect past developmental stress exposure, developmental food restriction impairs female reproductive success in a short-lived passerine.</p></div>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 5","pages":"546-553"},"PeriodicalIF":1.9,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduced Water Availability to Mothers and Embryos Has Little Effect on Offspring Phenotypes in an Invasive Lizard","authors":"Sydney M. Wayne,&nbsp;M. Christopher Norris,&nbsp;Amélie Fargevieille,&nbsp;Kerry Cobb,&nbsp;Cindy Scruggs,&nbsp;Jocelyn Miracle,&nbsp;Victoria Herron,&nbsp;Joshua M. Hall,&nbsp;Daniel A. Warner","doi":"10.1002/jez.2906","DOIUrl":"10.1002/jez.2906","url":null,"abstract":"<div>\u0000 \u0000 <p>Water availability has critical impacts on numerous physiological functions in all organisms and can have different effects across life stages. For example, water available to reproductive females can influence offspring production, and has the potential to influence how early-stage offspring (e.g., embryos) respond to moisture in their developmental environment. In this study, we use the brown anole lizard (<i>Anolis sagrei</i>) to examine the effects of water availability on reproductive investment. We tested the hypothesis that water available to mothers influences embryonic development under different levels of moisture in their incubation environment. We demonstrate that a substantial reduction in water availability to mothers (~70% reduction) has no observable effect on fecundity, egg size, or egg hydration. We also provide evidence that our water restriction to mothers does not influence how embryos respond to moisture conditions during egg incubation. However, moisture in the egg incubation substrate positively affected egg water uptake, egg hatching success and hatchling body size (but not hatchling hydration). Overall, our study shows that reproduction in adult female <i>A. sagrei</i> is robust to variation in water availability, but survival during early life stages is sensitive to this variable. Thus, the effect of water availability on different fitness components varies between life stages.</p></div>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 5","pages":"535-545"},"PeriodicalIF":1.9,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143066121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}