Journal of experimental zoology. Part A, Ecological and integrative physiology最新文献

{"title":"Using an Oral Sugar Test to Biologically Validate the Use of a Commercial Enzyme Immunoassay to Measure Salivary Insulin in Western Lowland Gorillas (Gorilla gorilla gorilla).","authors":"Kaylin S Tennant, Elena H Less, Mary Ann Raghanti, Laura A Amendolagine, Kristen E Lukas, Mark A Willis, Patricia M Dennis","doi":"10.1002/jez.2907","DOIUrl":"https://doi.org/10.1002/jez.2907","url":null,"abstract":"<p><p>Noninvasive evaluations of hormones can contribute to the assessment of health and welfare of animals. Variations in insulin levels and sensitivity, for example, have been linked to health concerns in non-human and human primates including insulin resistance, diabetes, and heart disease, the leading cause of death in zoo-housed gorillas. Few published studies have assessed insulin concentrations in western lowland gorillas (Gorilla gorilla gorilla), and all did so using serum. Anesthesia is typically required to collect blood samples from zoo-housed gorillas, limiting sampling frequency and restricting samples to the fasted state. The ability to measure insulin levels in saliva would eliminate these constraints and provide a minimally invasive means for monitoring this hormone. The purpose of this study was to analytically and biologically validate the measurement of insulin in saliva of western lowland gorillas using a commercially available enzyme immunoassay. For validation, an oral sugar test was conducted on five adult gorillas residing at Cleveland Metroparks Zoo. Such assessments are common practice in both human and equine medicine to evaluate the body's insulin response to ingestion of sugars. The test involved measuring salivary insulin at timed intervals before and after gorillas consumed doses of sugar. Salivary insulin levels increased from 15 min post-sugar ingestion and peaked after 90 to 120 min. One female had a high response and peaked at 990.21 mU/L. The other four gorillas had peaks between 49.82 and 167.04 mU/L. The assessment provided a biological validation for the measurement of salivary insulin in western lowland gorillas.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibodies and Inflammation: Fecal Biomarkers of Gut Health in Domestic Ruminants.","authors":"A M Burton, K J Else, J Irving, I Mair, S Shultz","doi":"10.1002/jez.2896","DOIUrl":"https://doi.org/10.1002/jez.2896","url":null,"abstract":"<p><p>Gastrointestinal infections present major challenges to ruminant livestock systems, and gut health is a key constraint on fitness, welfare, and productivity. Fecal biomarkers present opportunities to monitor animal health without using invasive methods, and with greater resolution compared to observational metrics. Here we developed enzyme-linked immunosorbent assays for three potential fecal biomarkers of gut health in domestic ruminants: two immunological (total immunoglobulin [Ig]A and total IgG) and one inflammatory (lactoferrin). We analytically validated the assays, then evaluated whether they could be used as a biomarker of clinically diagnosed gastrointestinal pathologies in cattle (Bos taurus), and finally compared them with helminth fecal egg counts in sheep (Ovis aries). The analytes were detected above the lower limits of detection in cattle, sheep, and goats. Fecal IgA and lactoferrin were higher in cattle with infectious pathologies (strongyles, coccidiosis and symptomatic Johne's disease) compared to healthy controls. Lactoferrin was additionally higher in animals with infectious pathologies compared to noninfectious pathologies, and to asymptomatic Johne's cases. No significant relationships were found with sheep fecal egg counts. These initial findings suggest that fecal IgA and lactoferrin may be useful biomarkers of poor gastrointestinal health in cattle, and that fecal lactoferrin is specific to active inflammation caused by infectious agents. These could be incorporated into the growing suite of noninvasive ecoimmunological tools and used to understand ruminant gut health in a range of species. Applications include improving treatment regimens for gastrointestinal infections, and understanding wildlife physiological responses to infectious challenges.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prenatal Corticosterone Impacts Nestling Condition and Immunity in Eastern Bluebirds","authors":"Taylor M. Miller,&nbsp;Kristen J. Navara","doi":"10.1002/jez.2898","DOIUrl":"10.1002/jez.2898","url":null,"abstract":"<p>Exposure of avian mothers to stressful conditions permanently alters offspring behavior and physiology. Yet, the effects of maternal stress on the development of offspring immunity in birds remain unclear, particularly in wild species. We injected Eastern bluebird (<i>Sialia sialis</i>) eggs with either a corticosterone or control solution, then measured the impacts on nestling morphology and two measures of immunity, bactericidal capacity and swelling responses to phytohemagglutinin. Nestlings from corticosterone-treated eggs had lower condition indices at hatch but quickly caught up to their control counterparts by Day 5 posthatch and until fledging. Corticosterone-exposed nestlings also mounted smaller swelling responses to phytohemagglutinin, whereas there were no effects on bactericidal capacity. These results indicate that maternal stress can impact offspring immunocompetence, fitness prospects, and potentially their ability to fend off parasites and pathogens.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 3","pages":"427-437"},"PeriodicalIF":1.9,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jez.2898","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circadian Modulation of Behavioral Stress Responses in Zebrafish Is Age-Dependent.","authors":"Santiago Pintos, Tyrone Lucon-Xiccato, Luisa María Vera, Francisco Javier Sánchez-Vázquez, Cristiano Bertolucci","doi":"10.1002/jez.2905","DOIUrl":"https://doi.org/10.1002/jez.2905","url":null,"abstract":"<p><p>In the wild, stressors occur with varying likelihood throughout the day, leading animals to evolve plastic stress responses that exhibit circadian rhythmicity. In mammals, studies have revealed that the circadian plasticity of stress response may differ with age. However, such developmental effects have been largely overlooked in other vertebrate groups. In our research, we explored the presence of developmental variation in the daily pattern of behavioral stress response in a teleost fish model: the zebrafish (Danio rerio). We compared juvenile and adult individuals in two behavioral paradigms commonly used to analyze fish stress response, such as the open-field test and the diving test. Our comparisons were conducted every 4 h during a 24-h cycle to analyze daily variations. Significant daily rhythms were detected for almost all analyzed behaviors in both tests. In general, the analyses suggested a greater stress response in adults during the daytime and in juveniles during the night-time, although not all indicators aligned in this direction. Moreover, we found average differences in zebrafish behavior, suggesting that juveniles were more sensitive to stress. Overall, these findings highlight the importance of considering developmental variation in the circadian pattern of stress response in non-mammalian species like zebrafish.</p>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental Challenges and Co-Infection Modulate Resistance and Tolerance Against Trypanosoma Cruzi and Trichinella Spiralis in Rats","authors":"Cintia C. Palavecino,&nbsp;Pablo F. Cuervo,&nbsp;María C. Fantozzi,&nbsp;Ivan A. Bontempi,&nbsp;Marcelo F. Ruiz,&nbsp;Rafael E. Marengo,&nbsp;Pablo M. Beldomenico,&nbsp;Andrea L. Racca","doi":"10.1002/jez.2902","DOIUrl":"10.1002/jez.2902","url":null,"abstract":"<div>\u0000 \u0000 <p>To overcome infection, hosts employ two defense strategies: resistance (which limits pathogen fitness), and tolerance (which reduces infection damage). These strategies may be influenced by environmental challenges such as food shortage, social conflict, and co-infections. Here, our objective was to assess defense strategies in rats infected with <i>Trichinella spiralis</i> and/or <i>Trypanosoma cruzi</i> under environmental challenges. After four weeks of treatment with environmental challenges (food restriction [0/1] and/or social conflict [0/1]), rats were exposed to <i>Tri. spiralis</i> [0/1] and/or <i>Try. cruzi</i> [0/1]. Six weeks postinoculation, we measured parasite intensity and several indicators of health or pathology. Tolerance to <i>Try. cruzi</i> increased in the presence of social conflict and food restriction. Coinfected animals showed reduced tolerance compared to mono-infected. However, food-restricted mono-infected rats had lower tolerance than other groups. No significant differences were found in resistance to <i>Try. cruzi</i>. Tolerance to <i>Tri. spiralis</i> was higher in food-restricted rats and Coinfected rats. Moreover, we found a potential shift in defense strategy: rats that are mono-infected and exposed to social conflict may be more resistant but less tolerant to <i>Tri. spiralis</i> than all other experimental groups. Overall, our findings highlight that defense strategies are context-dependent in the nematode-protozoan infection model studied, and provide evidence of a shift in the defense strategy to accommodate during environmental challenges. Given that rodents play a key role as reservoirs of zoonotic pathogens, understanding the range and variability of defense strategies in these animals is of utmost importance.</p></div>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 3","pages":"438-451"},"PeriodicalIF":1.9,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Body Temperature Regulation in Domestic Dogs After Agility Trials: The Effects of Season, Training, Body Characteristics, Age, and Genetics","authors":"William Andrew Russel,&nbsp;Ana Gabriela Jiménez,&nbsp;Kailey D. Paul,&nbsp;Barbara C. Hoopes,&nbsp;Ahmet Ay","doi":"10.1002/jez.2899","DOIUrl":"10.1002/jez.2899","url":null,"abstract":"<div>\u0000 \u0000 <p>An animal's body mass is said to be indirectly related to its rate of heat loss; that is, smaller animals with higher surface area to volume tend to lose heat faster than larger animals. Thus, thermoregulation should be related to body size, however, generalizable patterns are still unclear. Domestic dogs are a diverse species of endothermic mammals, including a 44-fold difference in body size. Previous work in sedentary dogs has determined that body size and other morphological variables tend to predict the thermoregulation of exercising pet dogs. Here, we aimed to address three questions: (1) whether thermoregulatory differences in domestic dogs across seasons are dictated strictly by external environmental temperatures or if individual thermal acclimation is affected by seasonal temperature variation, even indoors; (2) whether athleticism (or training experience) affects or changes thermoregulation in dogs, as it does in humans; and (3) whether thermoregulation in domestic dogs has a genetic basis. We obtained tympanic membrane (T_ear) temperatures and thermal images to measure the rate of temperature change in the eyes, mouth, and nose of athletic dogs following an indoor agility trial. Additionally, we used image analysis to determine body morphology differences. We found body mass to play a strong role in thermoregulation in winter trials (T_mouth <i>p</i> = 0.017, T_nose <i>p</i> = 0.052) but a less determinate role in summer trials. We found distinct differences in thermoregulation patterns between winter and summer. Particularly, coat morphology and length may play different roles in thermoregulation across seasons. Additionally, we found that rates of mouth temperature change differ by an interaction between environmental temperature and training experience (<i>p</i> = 0.044), suggesting seasonal thermoregulation patterns in dogs depend on relative athleticism. Lastly, we found important genetic predictors of temperature change rate, such as GORAB and IGF1, as well as others that exert influence over body size, mitochondrial function, or coat characteristics. These genetic markers indicate markers similar to our whole-animal physiological results. Overall, our data suggest that domestic dogs demonstrate thermal acclimation across seasons, that athleticism changes thermoregulatory patterns in domestic dogs, and that body size-related genes are associated with thermoregulation in dogs.</p></div>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 3","pages":"400-415"},"PeriodicalIF":1.9,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Muscle and Metabolic Genes Are Differentially Expressed During Thermal Acclimation by the Brook Trout Myotome","authors":"David J. Coughlin,&nbsp;Madeline D. Dutterer,&nbsp;Zachary D. LaMonica,&nbsp;Evelyn M. Peyton,&nbsp;Elizabeth S. Kwon,&nbsp;Kathleen A. Hittle","doi":"10.1002/jez.2901","DOIUrl":"10.1002/jez.2901","url":null,"abstract":"<div>\u0000 \u0000 <p>Cold-water fishes, such as Brook trout (<i>Salvelinus fontinalis</i>), are being challenged by the consequences of climate change. The ability of these fish to acclimate to warmer environmental conditions is vital to their survival. Acclimation to warmer water may allow brook trout to reduce the metabolic costs of higher temperatures. Previous work has shown that brook trout display a significant thermal acclimation response in their myotomal muscle, with slower contractile properties observed in warm acclimated fish. In this study, gene expression was examined in hatchery brook trout acclimated to a range in temperatures (4, 10 or 20°C). Brook trout displayed variations in gene expression in their myotomal muscle in accordance with acclimation temperature. Genes important for muscle function, cellular metabolism, protein degradation, and stress response showed variation to both warm (20°C) and cold (4°C) acclimation. The warm acclimated fish also showed decreased expression of genes associated with aerobic metabolism and increased expression of genes for heat shock proteins, while the cold acclimated fish showed increased expression of genes associated with lipid metabolism and protein turnover. α-tubulin displayed a close association with thermal acclimation, increasing in expression with acclimation temperature. The patterns of muscle gene expression were the opposite of what was expected. Although warm acclimated fish have previously been shown to display slow muscle contractile properties, this study found that warm acclimation is associated with increased expression of genes for kinetically faster isoforms of important muscle proteins. Collectively, the results demonstrate a robust response to elevated temperature in the hatchery fish greater than 10,000 genes showing differential expression with temperature. These results provide a roadmap for the analysis of the acclimation response of native populations of brook trout encountering climate change.</p></div>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 3","pages":"416-426"},"PeriodicalIF":1.9,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Body Condition and Blood Biochemistry of Free-Range Caiman latirostris in Northeast Brazilian Atlantic Forest","authors":"Gabriela Mota Gama,&nbsp;Luiza Figueiredo Passos,&nbsp;Adriano Pereira Paglia,&nbsp;Marcos Eduardo Coutinho","doi":"10.1002/jez.2897","DOIUrl":"10.1002/jez.2897","url":null,"abstract":"<div>\u0000 \u0000 <p>The Atlantic Forest broad-snouted caiman (<i>Caiman latirostris</i>) inhabits regions within one of the world's most ecologically diverse ecosystems, yet few studies have explored the relationship between body condition, blood biochemistry, and environmental factors in the wild. Our study investigated the effects of sex, ontogeny, habitat, and environmental variables on the body condition and blood biochemistry of free-ranging caimans from the state of Alagoas, Northeast Brazil. From 2020 to 2022, we captured 75 caimans across three sites in different seasons. Results revealed sex-specific responses to seasonal and Interannual weather changes, with females showing higher body condition in the wet season, while males peaked in the dry season. Elevated glucose, total protein, albumin, triglycerides, and fructosamine were linked to higher body condition and larger individuals, while elevated aspartate aminotransferase to low body condition. Seasonal rainfall influenced blood parameters, with the dry season associated with higher creatinine, calcium, and alanine aminotransferase levels, and the wet season with higher total protein, sodium, and potassium. Differences in glucose, alkaline phosphatase, and gamma-glutamyl transferase across sites pointed to physiological effects of human activities. Blood biochemical values varied widely, with some exceeding reported species ranges. These findings highlight the need to interpret physiological data within the context of local habitat and environmental conditions. Conservation strategies should go beyond species presence and habitat preservation, incorporating pollution control. Our study advances understanding of <i>Caiman latirostris</i> ecophysiology, offering valuable insights for the conservation and management of crocodilian populations in both wild and captive environments.</p></div>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 3","pages":"383-399"},"PeriodicalIF":1.9,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142970997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant and Anti-Inflammatory Impacts of Soft Tissue Crude Extract and Mucous of Snail Helix aspersa on an Excision Wound Model in Mice","authors":"Hoda H. Abdel-Azeem,&nbsp;Gamalat Y. Osman,&nbsp;Dalia S. Morsi","doi":"10.1002/jez.2895","DOIUrl":"10.1002/jez.2895","url":null,"abstract":"<div>\u0000 \u0000 <p>Wound healing is a complex natural process in which tissue requires recovering injured tissue cells. <i>Helix aspersa</i> has a high nutritional value and is considered a rich natural source of antioxidants and anti-inflammatory agents. So, this study aimed to assess the effect of soft tissue crude extract and mucous of <i>H. aspersa</i> topically applied as a gel for 12 days. The wounds were observed and photographed twice a week. The inflammatory, oxidative stress markers and matrix metalloproteinases were evaluated in skin tissue homogenate and CD3⁺ and CD69⁺ T lymphocytes were detected in wound tissue. Data showed that a comparison of applying soft tissue crude extract and mucous of <i>H. aspersa</i> to skin wounds enhanced the healing process, resulting in a significant decrease in dermal inflammation compared to untreated mice. Also, they significantly increased the antioxidant enzyme activities with reduced malondialdehyde (MDA) levels in wound tissues. The levels of matrix metalloproteases-2 and -9 were significantly decreased and the immune status was enhanced in the wound environment by increasing proportions of CD3⁺ and CD69⁺ T lymphocytes. <i>H. aspersa</i> mucous and soft tissue crude extract are viable substitutes for synthetic topical wound therapies with anti-inflammatory, antioxidant, and immunomodulatory potencies, with a preference for the crude soft tissue extract based on the outcomes.</p></div>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 3","pages":"373-382"},"PeriodicalIF":1.9,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142970991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of Branchial Anoctamin 1 Expression in Freshwater- and Seawater-Acclimated Japanese Medaka, Oryzias latipes","authors":"Norifumi Konno,&nbsp;Ayane Togashi,&nbsp;Hiroshi Miyanishi,&nbsp;Morio Azuma,&nbsp;Tomoya Nakamachi,&nbsp;Kouhei Matsuda","doi":"10.1002/jez.2894","DOIUrl":"10.1002/jez.2894","url":null,"abstract":"<div>\u0000 \u0000 <p>In euryhaline teleosts, the cystic fibrosis transmembrane conductance regulator (CFTR) in seawater (SW)-type chloride cells facilitates apical Cl⁻ secretion for SW adaptation, while alternative Cl⁻ excretion pathways remain understudied. This study investigates the role of the calcium-activated chloride channel, Anoctamin 1 (ANO1), in the gills of the euryhaline Japanese medaka (<i>Oryzias latipes</i>) under hyperosmolality and cortisol (CORT) influence. Acclimation to artificial SW, NaCl, mannitol, or glucose significantly upregulated <i>ANO1</i> and <i>CFTR</i> mRNA expression in gills, unlike urea treatment. In situ hybridization revealed <i>ANO1</i> mRNA in chloride cells co-expressing <i>CFTR</i> and <i>Na</i>⁺, <i>K</i>⁺-ATPase under hyperosmotic conditions. ANO1 inhibition elevated plasma Cl⁻ concentration, indicating impaired Cl⁻ excretion. CORT or dexamethasone administration in freshwater (FW) fish significantly increased branchial <i>ANO1</i> and <i>CFTR</i> mRNA expression, an effect attenuated by the glucocorticoid receptor (GR) antagonist RU486. Hyperosmotic treatment of isolated gill tissues rapidly induced <i>ANO1</i> mRNA expression independent of <i>CFTR</i> mRNA changes, and this induction was unaffected by RU486. These findings highlight the dual regulation of ANO1 expression via hyperosmolality-induced cellular response and the CORT–GR system. Thus, branchial ANO1 may likely complement CFTR in Cl⁻ excretion, playing a key role in the hyperosmotic adaptation of euryhaline teleosts.</p></div>","PeriodicalId":15711,"journal":{"name":"Journal of experimental zoology. Part A, Ecological and integrative physiology","volume":"343 3","pages":"356-372"},"PeriodicalIF":1.9,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}