Journal of endodontics最新文献

{"title":"Leukemia and Lymphoma Mimicking Periapical Conditions Resulting in Endodontic Treatment: A Systematic Review","authors":"Isis Samara de Melo Queiroga DDS ,&nbsp;Victor Zanetti Drumond DDS ,&nbsp;Lucas Guimarães Abreu DDS, MSc, PhD ,&nbsp;Lauren Frenzel Schuch DDS, MSc, PhD ,&nbsp;Ricardo Alves Mesquita DDS, MSc, PhD ,&nbsp;Erick Miranda Souza DDS, MSc, PhD ,&nbsp;Bruno Augusto Benevenuto de Andrade DDS, MSc, PhD ,&nbsp;José Alcides Almeida de Arruda DDS, MSc, PhD ,&nbsp;Gerhilde Callou Sampaio DDS, MSc, PhD","doi":"10.1016/j.joen.2024.11.005","DOIUrl":"10.1016/j.joen.2024.11.005","url":null,"abstract":"<div><h3>Introduction</h3><div>Leukemia and lymphoma mimicking periapical conditions can lead to significant diagnostic pitfalls. Guidance for endodontists in managing such cases is barely explored. This systematic review aimed to summarize the clinicopathologic, imaging, and management aspects of leukemia/lymphoma that mimicked periapical conditions and resulted in endodontic treatment.</div></div><div><h3>Methods</h3><div>Searches were conducted in PubMed, Embase, Scopus, Web of Science, and LILACS, supplemented by manual scrutiny and gray literature. Case reports and/or case series were included. Critical appraisal of the studies was performed using the Joanna Briggs Institute tool.</div></div><div><h3>Results</h3><div>Among 3,617 identified records, 32 studies involving 37 individuals (mean age 43.6 years; male-to-female ratio 1.2:1) were included. Diffuse large B-cell lymphoma was diagnosed in 21 (56.8%) individuals. The anterior maxilla and posterior mandible were the most frequently affected sites (29.7% each). Radiographically, 97.3% of the lesions exhibited radiolucency in the periapical region. Endodontic treatment preceded the diagnosis of leukemia/lymphoma in 94.6% of cases, while retreatment occurred in 5.4%. The mean time to final diagnosis was 4.9 months.</div></div><div><h3>Conclusion</h3><div>Leukemia and lymphoma can be misdiagnosed as periapical conditions, leading to inappropriate endodontic treatments and delayed diagnoses. Endodontists should become familiar with the broad clinicoradiographic spectrum of these rare, but potentially life-threatening hematolymphoid malignancies.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 2","pages":"Pages 106-117"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adrenomedullin-loaded Gelatin Methacryloyl Hydrogel Promotes Endogenous Dental Pulp Regeneration: An In Vitro and In Vivo Study","authors":"Yangpeng Zhao MD ,&nbsp;Qian Zhang MD ,&nbsp;Song Zhang MD ,&nbsp;Jianan Chen MD ,&nbsp;Lingtong Kong MD ,&nbsp;Jianyong Gao MD ,&nbsp;Qiang Zhu MD","doi":"10.1016/j.joen.2024.11.011","DOIUrl":"10.1016/j.joen.2024.11.011","url":null,"abstract":"<div><h3>Introduction</h3><div>To prepare a gelatin methacryloyl (GelMA) hydrogel scaffold embedded with adrenomedullin (ADM) and investigate its impact and underlying mechanisms in endogenous pulp regeneration.</div></div><div><h3>Methods</h3><div>ADM was evenly distributed within the GelMA hydrogel through a simple and conventional physical mixing technique. The scaffold underwent characterization via scanning electron microscopy, alongside assessments of swelling, degradation, and release properties. Biocompatibility was evaluated using cytoskeletal and live-dead staining techniques. The hydrogel's influence on dental pulp stem cells' proliferation, migration, and differentiation was assessed with CCK-8 assays, Transwell assays, and alizarin red and alkaline phosphatase staining. Transcriptomics provided insights into potential mechanisms. The angiogenic effects on umbilical vein endothelial cells were examined using scratch and tube formation assays. <em>In vivo</em>, the composite hydrogel's regenerative capacity was tested in a rat model of pulp regeneration. Statistical analysis involved Student's <em>t</em>-test and one-way analysis of variance, with significance set at <em>P</em> &lt; .05.</div></div><div><h3>Results</h3><div>The ADM-loaded GelMA (GelMA@ADM) hydrogel displayed a porous architecture under electron microscopy conducive to cell adhesion and demonstrated excellent biocompatibility. <em>In vitro</em> experiments showed that GelMA@ADM significantly boosted dental pulp stem cells' migration, proliferation, and differentiation, and enhanced the angiogenic activity of umbilical vein endothelial cells after one week of treatment. Corresponding <em>in vivo</em> experiments revealed that GelMA@ADM facilitated the formation of new vascularized pulp tissue after 2 weeks of treatment.</div></div><div><h3>Conclusions</h3><div>The GelMA@ADM hydrogel effectively promotes dental pulp stem cells' proliferation and differentiation, augments vascularization by umbilical vein endothelial cells, and fosters the creation of new vascularized pulp tissue. These findings underscore the potential of GelMA@ADM hydrogel for endogenous pulp regeneration.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 2","pages":"Pages 172-184"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Debris Apical Extrusion, Unprepared Surface Areas, and Dentinal Crack Formation after Root Canal Preparation Using ProTaper Next, ProTaper Ultimate, and R-Motion File Systems","authors":"Haider A. Ibrahim BDS ,&nbsp;Ahmed H. Ali BDS, MSc, PhD (UK) ,&nbsp;Francesco Mannocci MD, DDS, PhD, FHEA","doi":"10.1016/j.joen.2024.11.014","DOIUrl":"10.1016/j.joen.2024.11.014","url":null,"abstract":"<div><h3>Introduction</h3><div>This study evaluated and compared the amount of apically extruded debris, unprepared wall surface areas, and dentinal crack formation resulting from root canal preparation using ProTaper Next (PTN), ProTaper Ultimate (PTU), and R-Motion (RM) file systems.</div></div><div><h3>Methods</h3><div>Forty-five palatal roots of maxillary first molars were shortened to a uniform length of 12 mm. The samples were divided randomly into 3 groups (<em>n</em> = 15/group) to be prepared using either PTN, PTU, and RM file systems. The investigator adopted modified Myers and Montgomery's approach to replicate the temperature of the human body. Debris was collected in vials and weighed before and after instrumentation with a sensitive balance. Every specimen was irrigated using distilled water during preparation, utilizing a side-vented needle. Weight before instrumentation was subtracted from weight after instrumentation to identify the weight of the collected debris. Cone-beam computed tomography (CBCT) scans were taken before and after canal preparation using a Planmeca ProMax CBCT machine. The unprepared canal surface area was calculated by superimposing preoperative and postoperative CBCT images using the Mimics Medical program. After preparation, a low-speed diamond disc horizontally sectioned the roots at 3, 6, and 9 millimeters from the apex. The specimens were examined under a digital microscope to detect crack formation. Statistical analysis was carried out using one-way (ANOVA) and Tukey (HSD) tests, with a significance level of <em>P</em> &lt; .05.</div></div><div><h3>Results</h3><div>There was no statistically significant difference in the quantity of debris extruded apically and dentinal crack numbers among the 3 shaping files (<em>P</em> &gt; .05). Only the apical third of root canals prepared by PTU displayed a significantly lower unprepared surface area compared to PTN and RM.</div></div><div><h3>Conclusions</h3><div>Instrumentation with PTN, PTU, and RM yielded similar amounts of apically extruded debris and dentinal cracks. The PTU files demonstrated higher effectiveness in preparing the apical third by reducing the unprepared surface area in the prepared root canal.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 2","pages":"Pages 200-206"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of Apical Periodontitis with Glycated Hemoglobin Levels and Systemic Inflammatory Markers in Patients with Type 2 Diabetes: A Cross-Sectional Study","authors":"Ritika Dhamija MDS ,&nbsp;Sanjay Tewari MDS ,&nbsp;Paramjeet Singh Gill MD ,&nbsp;Nitika Monga MDS ,&nbsp;Shweta Mittal MDS ,&nbsp;Jigyasa Duhan MDS","doi":"10.1016/j.joen.2024.11.008","DOIUrl":"10.1016/j.joen.2024.11.008","url":null,"abstract":"<div><h3>Introduction</h3><div>This cross-sectional study, as a preliminary part of an ongoing project, aimed to investigate the effect of apical peridontitis (AP) on glycated hemoglobin (HbA1c) and systemic inflammatory markers in diabetic individuals.</div></div><div><h3>Methods</h3><div>A total of 280 individuals (140 with type 2 diabetes mellitus [T2DM] and 140 healthy) with and without AP were enrolled. Sixty-four age-, gender-, and body mass index–matched participants each in T2DM with AP group (DAP), T2DM without apical periodontitis (D), systemically healthy controls with apical periodontitis (CAP), and without apical periodontitis (C) groups were evaluated. Radiologic and clinical oral examination was performed for confirming the diagnosis of AP and periapical index scoring (PAI). Blood analyses were carried out for interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, high-sensitivity C-reactive protein (hsCRP), and HbA1c assessment.</div></div><div><h3>Results</h3><div>Significantly higher levels of IL-1β, TNF-α, and hsCRP were observed in patients with AP in both diabetes and control groups (<em>P</em> &lt; .05). In the diabetes group, AP contributed to significantly raised levels of HbA1c compared with T2DM group patients without AP. After controlling for possible confounders, partial corelation coefficients revealed positive corelation of presence of AP as well as size of the periapical lesion with HbA1c and serum levels of inflammatory markers in both diabetic and healthy individuals. Multivariate linear regression analysis revealed both presence of AP (<em>P</em> &lt; .05) as well as the size of lesion (<em>P</em> &lt; .001) were found to significantly predict the HbA1c levels as well as the levels of IL-1β, IL-6, TNF-α, and hsCRP in both diabetic and non-diabetic individuals.</div></div><div><h3>Conclusions</h3><div>These findings suggest that both presence of AP and size of periapical lesion was associated with glycemic control and systemic inflammatory burden in patients with T2DM.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 2","pages":"Pages 124-131"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into the February 2025 Issue of the JOE","authors":"Anita Aminoshariae DDS, MS,&nbsp;Amir Azarpazhooh DDS, MSc, PhD, FRCD(C),&nbsp;Gerald N. Glickman DDS, MS, MBA, JD,&nbsp;Jianing He DMD, PhD,&nbsp;Sahng G. Kim DDS, MS,&nbsp;Anil Kishen BDS, MDS, PhD,&nbsp;Ariadne M. Letra DDS, MS, PhD,&nbsp;Linda Levin DDS, PhD,&nbsp;Ronald Ordinola-Zapata,&nbsp;Frank C. Setzer DMD, PhD, MS,&nbsp;Franklin R. Tay BDSc(Hons), PhD,&nbsp;Kenneth M. Hargreaves DDS, PhD","doi":"10.1016/j.joen.2025.01.017","DOIUrl":"10.1016/j.joen.2025.01.017","url":null,"abstract":"","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 2","pages":"Pages 103-105"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peripheral Lysosomal Positioning in Inflamed Odontoblasts Facilitates Mineralization","authors":"Nuo Xu DDS,&nbsp;Qian Gao DDS,&nbsp;Chengcan Yang DDS, PhD,&nbsp;Xiaona Song BDS,&nbsp;Kai Yang DDS, PhD,&nbsp;Zhuan Bian DDS, PhD","doi":"10.1016/j.joen.2024.11.006","DOIUrl":"10.1016/j.joen.2024.11.006","url":null,"abstract":"<div><h3>Introduction</h3><div>Odontoblasts, terminally differentiated dentin-producing cells, critically rely on lysosomal functions for intracellular recycling and renewal. Beyond their traditional degradative role, lysosomes actively orchestrate cellular responses to external stimuli through precise and rapid intracellular trafficking and positioning. This study aimed to explore the influence of lysosomal positioning on odontoblast mineralization and the underlying mechanisms implicated in carious inflammation.</div></div><div><h3>Methods</h3><div>Human dental pulp stem cells were induced to differentiate into human odontoblast-like cells (hOBLCs). hOBLCs were treated with various doses of LPS (0.1, 1, 5 μg/mL) to mimic carious inflammation. Lysosomal positioning was examined by immunofluorescence staining of lysosomal associated membrane protein 1 in healthy and carious human teeth, LPS-treated hOBLCs, mouse lower incisors at postnatal day 2.5, and mineralization medium cultured human dental pulp stem cells. Lysosomal positioning was manipulated by knockdown or overexpression of <em>SNAPIN</em> or <em>ARL8B</em>. Mineralization was assessed by ARS staining and expression of DSPP and DMP1. Lysosomal exocytosis was examined by detection of lysosomal-plasma membrane fusion, surface exposure of lysosomal associated membrane protein 1 luminal epitopes (1D4B), and extracellularly released lysosomal enzymes.</div></div><div><h3>Results</h3><div>Peripheral lysosomal positioning was markedly increased in odontoblasts within moderate and extensive carious lesions (<em>P</em> &lt; .001) and in hOBLCs following LPS treatment. Increased peripheral dispersion of lysosomes was similarly observed during odontoblastic differentiation <em>in vivo</em> and <em>in vitro</em>. Moreover, peripheral lysosomal positioning promoted mineralization in inflamed hOBLCs, potentially via mTORC1 signaling pathway and lysosomal exocytosis.</div></div><div><h3>Conclusion</h3><div>Inflammatory stimuli prompted a relocation of lysosomes in odontoblasts, redistributing them from perinuclear location toward the cell periphery, which in turn facilitated mineralization, potentially via mTORC1 signaling and lysosomal exocytosis.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 2","pages":"Pages 185-194"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Controlling Root Canal Infection in Oncological Patients with Apical Periodontitis","authors":"Kaline Romeiro PhD ,&nbsp;Luciana F. Gominho PhD ,&nbsp;Danielle D. Voigt PhD ,&nbsp;Ibrahimu Mdala PhD ,&nbsp;Isabela N. Rôças PhD ,&nbsp;José F. Siqueira Jr. PhD","doi":"10.1016/j.joen.2024.11.012","DOIUrl":"10.1016/j.joen.2024.11.012","url":null,"abstract":"<div><h3>Introduction</h3><div>This study evaluated the effectiveness of chemomechanical preparation associated with intracanal medication in reducing the total bacterial load of infected root canals in cancer patients undergoing antineoplastic treatment. The prevalence, levels, and response to treatment of <em>Streptococcus</em> and Actinobacteria species were also assessed.</div></div><div><h3>Methods</h3><div>The root canals of teeth with apical periodontitis from oncological and control patients were treated using rotary instrumentation, 2.5% NaOCl irrigation, and 2-week calcium hydroxide medication (<em>n</em> = 23/group). Bacteriological root canal samples were taken before (S1) and after chemomechanical preparation (S2), and after intracanal medication (S3). DNA extracted from the samples was subjected to 16S rRNA gene-based quantitative real-time polymerase chain reaction assays for evaluation of the treatment effects on total bacteria, <em>Streptococcus</em> species, and members of the Actinobacteria phylum.</div></div><div><h3>Results</h3><div>All S1 samples were positive for bacteria. S1–S2 and S1–S3 bacterial reduction values were highly significant (<em>P</em> &lt; .01). Although bacterial counts were reduced from S2 to S3, the decrease was not statistically significant (<em>P</em> &gt; .05). Intergroup comparisons showed no significant differences in S1, S2 and S3 counts (<em>P</em> &gt; .05). In the oncological group, 77% of the cases were positive for bacteria in S2, and 68% in S3. In the control group, corresponding figures were 65% for both S2 and S3. There were no significant differences between groups as for streptococci and actinobacteria prevalence, levels, and response to treatment (<em>P</em> &gt; .05).</div></div><div><h3>Conclusions</h3><div>Root canal treatment using rotary instruments, NaOCl irrigation, and calcium hydroxide medication was highly effective in reducing the bacterial populations in infected teeth with apical periodontitis from cancer patients, with no significant differences to controls.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 2","pages":"Pages 157-163"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the Impact of Ultrasonic Irrigation on Bacterial Levels and Activity Following Chemomechanical Procedures","authors":"Carlos Goes Nogales DDS, MSc, PhD ,&nbsp;Roberto Xavier Romero Cazares DDS, MSc, PhD ,&nbsp;Laura Cristina Leite Nardello DDS, MSc, PhD ,&nbsp;Marcia Pinto Alves Mayer DDS, MSc, PhD ,&nbsp;Giulio Gavini DDS, MSc, PhD ,&nbsp;Matthias Zehnder Dr. med. dent., PhD ,&nbsp;Ericka Tavares Pinheiro DDS, MSc, PhD","doi":"10.1016/j.joen.2024.09.001","DOIUrl":"10.1016/j.joen.2024.09.001","url":null,"abstract":"<div><h3>Introduction</h3><div>This single-arm interventional trial aimed to investigate the efficacy of ultrasonic irrigation as a supplementary disinfection approach after chemomechanical procedures (CMP) using molecular techniques based on ribosomal RNA (rRNA) and rRNA genes (referred to as DNA).</div></div><div><h3>Methods</h3><div>Samples were collected from 35 single-rooted teeth with radiographic evidence of apical periodontitis. Samples were taken after gaining root canal access (S1), (S2), and ultrasonic irrigation (S3). DNA-targeted quantitative polymerase chain reaction using universal primers was used to estimate total bacterial levels, while rRNA-targeted quantitative polymerase chain reaction was used to assess bacterial activity. Ratios between rRNA and DNA levels were calculated to search for active bacteria in the samples (rRNA/DNA ≥ 1). Wilcoxon matched-pairs signed-rank test was used to compare the differences in DNA levels between samples and DNA and rRNA levels within samples (<em>P</em> &lt; .05).</div></div><div><h3>Results</h3><div>DNA-based methods revealed a significant decrease in bacterial levels from S1 to S2 and S2 to S3 (both <em>P</em> &lt; .05). Notably, 11 out of 35 (31.4%) root canals did not harbor bacterial DNA after CMP, whereas ultrasonic activation increased DNA-negative samples to 17 (48.6%). However, all DNA-positive samples were also positive for rRNA, with significantly higher rRNA than DNA levels (<em>P</em> &lt; .05), indicating bacterial activity at the sampling time.</div></div><div><h3>Conclusions</h3><div>Ultrasonic irrigation improved the disinfection of root canals after CMP by reducing bacterial levels. However, persisting bacteria remained active in the root canals after CMP and ultrasonic irrigation.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 2","pages":"Pages 118-123"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142288958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Methodological Study on Microbial In Vivo Sampling Methods of Root Canal Microbiota for Next-Generation Gene Sequencing Analysis","authors":"David Donnermeyer PD Dr. med. dent. ,&nbsp;Johannes Matern Dr. med. dent. ,&nbsp;Karola Prior Dr. rer. medic. ,&nbsp;Madgalena Ibing Dr. med. dent. ,&nbsp;Daniel Hagenfeld PD Dr. med. dent. ,&nbsp;Edgar Schäfer Prof. Dr. med. dent. ,&nbsp;Sebastian Bürklein Prof. Dr. med. dent. ,&nbsp;Dag Harmsen Prof. Dr. med. ,&nbsp;Benjamin Ehmke Prof. Dr. med. dent.","doi":"10.1016/j.joen.2024.11.007","DOIUrl":"10.1016/j.joen.2024.11.007","url":null,"abstract":"<div><h3>Introduction</h3><div>The aim was to evaluate the suitability of paper points or endodontic nickel-titanium files to sample microorganisms for <em>in vivo</em> investigation of endodontic microbiota by 16S ribosomal DNA (rDNA) sequencing.</div></div><div><h3>Methods</h3><div>Forty-five patients presenting clinical and radiological signs of apical periodontitis were recruited for sampling, giving their written informed consent. Glide paths were assessed using C-Pilot Files and K-Files under electronic root canal length control under aseptic conditions. Microbial samples were taken from 84 root canals in duplicate, the first sample with a sterile paper point (size 15), the second with a sterile file (size 20/.06). After DNA extraction, the hypervariable region V4 of the bacterial 16 S rRNA gene was amplified and sequenced (Illumina MiSeq). Sequencing data were trimmed with Cutadapt and exact amplicon sequence variants generated by DADA2. Taxonomy was assigned based on the Human Oral Microbiome Database (eHOMD). Statistical analysis of diversity parameters comprised Wilcoxon signed-rank tests and permutational analysis of variance (PERMANOVA). Compositional differences were evaluated by differential abundance analysis (DESeq2). Microbial contamination during the sampling process and analysis were evaluated.</div></div><div><h3>Results</h3><div>Concerning alpha diversity, richness and dissimilarity differed nonsignificantly between paper point and instrument samples (<em>P</em> &gt; .05), whereas a significant difference was observed in the Shannon index (<em>P</em> &lt; .05). Regarding beta diversity, paper point and instrument samples presented with similar microbial community compositions (<em>P</em> = 1.0, PERMANOVA). Paper point controls contained significantly higher proportions of Pseudomonadales (<em>P</em> &lt; .05).</div></div><div><h3>Conclusions</h3><div>Paper point and endodontic instrument sampling generate valid specimens for 16S rDNA community profiling. Endodontic instrument sampling is easier to execute and, therefore, could be the technique of choice.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 2","pages":"Pages 164-171"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Management of Orofacial Trauma Including Multiple Avulsions, Lateral Luxation, and Alveolar Fracture: A Case Report with 7-Years of Follow-Up","authors":"Filipe Colombo Vitali DDS, MSc, PhD,&nbsp;Gabriela Ribeiro Sumar DDS,&nbsp;Julia Menezes Savaris DDS, MSc,&nbsp;Charles Marin DDS, MSc, PhD,&nbsp;Lucas da Fonseca Roberti Garcia DDS, MSc, PhD,&nbsp;Cleonice da Silveira Teixeira DDS, MSc, PhD","doi":"10.1016/j.joen.2024.11.015","DOIUrl":"10.1016/j.joen.2024.11.015","url":null,"abstract":"<div><div>The occurrence of different types of trauma concomitantly can complicate the establishment of the most appropriate treatment. This report describes the clinical management of orofacial trauma including multiple avulsions, lateral luxation, alveolar fracture, and their sequelae after 7 years of follow-up. A 19-year-old woman presented to emergency care 4 hours after an accidental fall that resulted in the avulsion of teeth #8, #9, and #25; a complicated crown fracture and lateral luxation of tooth #7; an uncomplicated crown fracture of tooth #10; and a fracture of the maxillary alveolar bone. Only teeth #8 and #9 were located by the patient. The immediate treatment included surgical reduction and extrusion of the lateral luxation, delayed replantation of the avulsed central incisors, nonrigid splint stabilization, and suturing of gingival lacerations. Subsequent visits involved removal of the splint, root canal treatment of the maxillary incisors, implant placement to replace the avulsed mandibular incisor, and esthetic rehabilitation with composite resin. At the 5-year follow-up, cone beam computed tomography revealed advanced surface root resorption in the replanted incisors. At the 7-year follow-up, an aggravation of inflammatory resorption in the replanted incisors was observed. This report demonstrates that adequate knowledge of trauma management by clinicians is essential for the effective management of more complex cases. It also highlights the importance of long-term follow-up in cases of avulsion and replantation due to potential long-term sequelae.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 2","pages":"Pages 213-221.e1"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}