Impact of Wine Polyphenols on the Inflammatory Profile of Induced Apical Periodontitis in Rats

IF 3.6 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of endodontics Pub Date : 2025-05-01 DOI:10.1016/j.joen.2025.01.023

Rafaela Ricci MSc , Bharbara de Moura Pereira MSc , Julissa Denisse Arguello Alvarado DDS , Romulo de Oliveira Sales-Junior MSc , Nathália Evelyn da Silva Machado PhD , Doany Cevada dos Santos DDS , Felipe Haddad Martim Pederro DDS , Marciane Magnani PhD , Marcos dos Santos Lima PhD , Edilson Ervolino PhD , Luciano Tavares Ângelo Cintra PhD , Anil Kishen PhD , João Eduardo Gomes-Filho PhD

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引用次数: 0

Abstract

Introduction

This study evaluated the impact of dealcoholized red wine polyphenols on the inflammation and lesion volume associated with apical periodontitis (AP) in rats.

Methods

Thirty-two Wistar rats receiving AP induction were arranged as follows: Control Group, Dealcoholized Red Wine Group (DRW), Red Wine Group, and Alcohol Group (ALC). Solutions were administered daily in a volume of 4.28 mL/kg via gavage for 45 days. Mandibles and maxillae were removed for histologic, immunohistochemical (IL-1β, IL-10, tumor necrosis factor-alpha, receptor activator of nuclear factor κB ligand, osteoprotegerin [OPG], and tartrate-resistant acid phosphatase), and micro-computed tomography analyses of the AP site. A statistical analysis was performed with a significance level of 5%.

Results

Inflammation and TRAP-positive cell count were similar for DRW and Red Wine Group, but lower when compared to Control Group and ALC (P < .001). The immunohistochemical expression of OPG was higher for DRW than for ALC (P < .05). A larger lesion volume was observed in ALC compared to other groups (P < .001).

Conclusions

Prophylactic administration of dealcoholized red wine significantly reduced inflammation, decreased the number of TRAP-positive cells, enhanced OPG expression, and reduced lesion volume compared to water and alcohol solutions.

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酒多酚对大鼠诱导的根尖牙周炎炎症的影响。

前言：本研究评价了脱醇红酒多酚对大鼠根尖牙周炎（AP）相关炎症和病变体积的影响。方法：将32只接受AP诱导的Wistar大鼠分为：对照组(C)、脱醇红酒组（DRW）、红酒组(W)和酒精组（ALC）。每天以4.28 mL/kg的体积给药，灌胃45天。取下颌骨进行AP部位的组织学、免疫组织化学（IL-1β、IL-10、TNF-α、RANKL、OPG、TRAP）和显微计算机断层扫描分析。进行统计学分析，显著性水平为5%。结果：DRW和W的炎症和trap阳性细胞计数相似，但与C和ALC相比较低。结论：与水和酒精溶液相比，预防性给予脱醇红葡萄酒可显著减轻炎症，减少trap阳性细胞数量，增强OPG表达，减少病变体积。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of endodontics 医学-牙科与口腔外科

CiteScore

8.80

自引率

9.50%

发文量

224

审稿时长

42 days

期刊介绍： The Journal of Endodontics, the official journal of the American Association of Endodontists, publishes scientific articles, case reports and comparison studies evaluating materials and methods of pulp conservation and endodontic treatment. Endodontists and general dentists can learn about new concepts in root canal treatment and the latest advances in techniques and instrumentation in the one journal that helps them keep pace with rapid changes in this field.