Journal of endodontics最新文献

{"title":"Insights into the February 2025 Issue of the JOE","authors":"Anita Aminoshariae DDS, MS, Amir Azarpazhooh DDS, MSc, PhD, FRCD(C), Gerald N. Glickman DDS, MS, MBA, JD, Jianing He DMD, PhD, Sahng G. Kim DDS, MS, Anil Kishen BDS, MDS, PhD, Ariadne M. Letra DDS, MS, PhD, Linda Levin DDS, PhD, Ronald Ordinola-Zapata, Frank C. Setzer DMD, PhD, MS, Franklin R. Tay BDSc(Hons), PhD, Kenneth M. Hargreaves DDS, PhD","doi":"10.1016/j.joen.2025.01.017","DOIUrl":"10.1016/j.joen.2025.01.017","url":null,"abstract":"","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 2","pages":"Pages 103-105"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peripheral Lysosomal Positioning in Inflamed Odontoblasts Facilitates Mineralization","authors":"Nuo Xu DDS,&nbsp;Qian Gao DDS,&nbsp;Chengcan Yang DDS, PhD,&nbsp;Xiaona Song BDS,&nbsp;Kai Yang DDS, PhD,&nbsp;Zhuan Bian DDS, PhD","doi":"10.1016/j.joen.2024.11.006","DOIUrl":"10.1016/j.joen.2024.11.006","url":null,"abstract":"<div><h3>Introduction</h3><div>Odontoblasts, terminally differentiated dentin-producing cells, critically rely on lysosomal functions for intracellular recycling and renewal. Beyond their traditional degradative role, lysosomes actively orchestrate cellular responses to external stimuli through precise and rapid intracellular trafficking and positioning. This study aimed to explore the influence of lysosomal positioning on odontoblast mineralization and the underlying mechanisms implicated in carious inflammation.</div></div><div><h3>Methods</h3><div>Human dental pulp stem cells were induced to differentiate into human odontoblast-like cells (hOBLCs). hOBLCs were treated with various doses of LPS (0.1, 1, 5 μg/mL) to mimic carious inflammation. Lysosomal positioning was examined by immunofluorescence staining of lysosomal associated membrane protein 1 in healthy and carious human teeth, LPS-treated hOBLCs, mouse lower incisors at postnatal day 2.5, and mineralization medium cultured human dental pulp stem cells. Lysosomal positioning was manipulated by knockdown or overexpression of <em>SNAPIN</em> or <em>ARL8B</em>. Mineralization was assessed by ARS staining and expression of DSPP and DMP1. Lysosomal exocytosis was examined by detection of lysosomal-plasma membrane fusion, surface exposure of lysosomal associated membrane protein 1 luminal epitopes (1D4B), and extracellularly released lysosomal enzymes.</div></div><div><h3>Results</h3><div>Peripheral lysosomal positioning was markedly increased in odontoblasts within moderate and extensive carious lesions (<em>P</em> &lt; .001) and in hOBLCs following LPS treatment. Increased peripheral dispersion of lysosomes was similarly observed during odontoblastic differentiation <em>in vivo</em> and <em>in vitro</em>. Moreover, peripheral lysosomal positioning promoted mineralization in inflamed hOBLCs, potentially via mTORC1 signaling pathway and lysosomal exocytosis.</div></div><div><h3>Conclusion</h3><div>Inflammatory stimuli prompted a relocation of lysosomes in odontoblasts, redistributing them from perinuclear location toward the cell periphery, which in turn facilitated mineralization, potentially via mTORC1 signaling and lysosomal exocytosis.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 2","pages":"Pages 185-194"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Controlling Root Canal Infection in Oncological Patients with Apical Periodontitis","authors":"Kaline Romeiro PhD ,&nbsp;Luciana F. Gominho PhD ,&nbsp;Danielle D. Voigt PhD ,&nbsp;Ibrahimu Mdala PhD ,&nbsp;Isabela N. Rôças PhD ,&nbsp;José F. Siqueira Jr. PhD","doi":"10.1016/j.joen.2024.11.012","DOIUrl":"10.1016/j.joen.2024.11.012","url":null,"abstract":"<div><h3>Introduction</h3><div>This study evaluated the effectiveness of chemomechanical preparation associated with intracanal medication in reducing the total bacterial load of infected root canals in cancer patients undergoing antineoplastic treatment. The prevalence, levels, and response to treatment of <em>Streptococcus</em> and Actinobacteria species were also assessed.</div></div><div><h3>Methods</h3><div>The root canals of teeth with apical periodontitis from oncological and control patients were treated using rotary instrumentation, 2.5% NaOCl irrigation, and 2-week calcium hydroxide medication (<em>n</em> = 23/group). Bacteriological root canal samples were taken before (S1) and after chemomechanical preparation (S2), and after intracanal medication (S3). DNA extracted from the samples was subjected to 16S rRNA gene-based quantitative real-time polymerase chain reaction assays for evaluation of the treatment effects on total bacteria, <em>Streptococcus</em> species, and members of the Actinobacteria phylum.</div></div><div><h3>Results</h3><div>All S1 samples were positive for bacteria. S1–S2 and S1–S3 bacterial reduction values were highly significant (<em>P</em> &lt; .01). Although bacterial counts were reduced from S2 to S3, the decrease was not statistically significant (<em>P</em> &gt; .05). Intergroup comparisons showed no significant differences in S1, S2 and S3 counts (<em>P</em> &gt; .05). In the oncological group, 77% of the cases were positive for bacteria in S2, and 68% in S3. In the control group, corresponding figures were 65% for both S2 and S3. There were no significant differences between groups as for streptococci and actinobacteria prevalence, levels, and response to treatment (<em>P</em> &gt; .05).</div></div><div><h3>Conclusions</h3><div>Root canal treatment using rotary instruments, NaOCl irrigation, and calcium hydroxide medication was highly effective in reducing the bacterial populations in infected teeth with apical periodontitis from cancer patients, with no significant differences to controls.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 2","pages":"Pages 157-163"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the Impact of Ultrasonic Irrigation on Bacterial Levels and Activity Following Chemomechanical Procedures","authors":"Carlos Goes Nogales DDS, MSc, PhD ,&nbsp;Roberto Xavier Romero Cazares DDS, MSc, PhD ,&nbsp;Laura Cristina Leite Nardello DDS, MSc, PhD ,&nbsp;Marcia Pinto Alves Mayer DDS, MSc, PhD ,&nbsp;Giulio Gavini DDS, MSc, PhD ,&nbsp;Matthias Zehnder Dr. med. dent., PhD ,&nbsp;Ericka Tavares Pinheiro DDS, MSc, PhD","doi":"10.1016/j.joen.2024.09.001","DOIUrl":"10.1016/j.joen.2024.09.001","url":null,"abstract":"<div><h3>Introduction</h3><div>This single-arm interventional trial aimed to investigate the efficacy of ultrasonic irrigation as a supplementary disinfection approach after chemomechanical procedures (CMP) using molecular techniques based on ribosomal RNA (rRNA) and rRNA genes (referred to as DNA).</div></div><div><h3>Methods</h3><div>Samples were collected from 35 single-rooted teeth with radiographic evidence of apical periodontitis. Samples were taken after gaining root canal access (S1), (S2), and ultrasonic irrigation (S3). DNA-targeted quantitative polymerase chain reaction using universal primers was used to estimate total bacterial levels, while rRNA-targeted quantitative polymerase chain reaction was used to assess bacterial activity. Ratios between rRNA and DNA levels were calculated to search for active bacteria in the samples (rRNA/DNA ≥ 1). Wilcoxon matched-pairs signed-rank test was used to compare the differences in DNA levels between samples and DNA and rRNA levels within samples (<em>P</em> &lt; .05).</div></div><div><h3>Results</h3><div>DNA-based methods revealed a significant decrease in bacterial levels from S1 to S2 and S2 to S3 (both <em>P</em> &lt; .05). Notably, 11 out of 35 (31.4%) root canals did not harbor bacterial DNA after CMP, whereas ultrasonic activation increased DNA-negative samples to 17 (48.6%). However, all DNA-positive samples were also positive for rRNA, with significantly higher rRNA than DNA levels (<em>P</em> &lt; .05), indicating bacterial activity at the sampling time.</div></div><div><h3>Conclusions</h3><div>Ultrasonic irrigation improved the disinfection of root canals after CMP by reducing bacterial levels. However, persisting bacteria remained active in the root canals after CMP and ultrasonic irrigation.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 2","pages":"Pages 118-123"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142288958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associate Registry","authors":"","doi":"10.1016/S0099-2399(25)00047-0","DOIUrl":"10.1016/S0099-2399(25)00047-0","url":null,"abstract":"","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 2","pages":"Pages 222-253"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143327940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Methodological Study on Microbial In Vivo Sampling Methods of Root Canal Microbiota for Next-Generation Gene Sequencing Analysis","authors":"David Donnermeyer PD Dr. med. dent. ,&nbsp;Johannes Matern Dr. med. dent. ,&nbsp;Karola Prior Dr. rer. medic. ,&nbsp;Madgalena Ibing Dr. med. dent. ,&nbsp;Daniel Hagenfeld PD Dr. med. dent. ,&nbsp;Edgar Schäfer Prof. Dr. med. dent. ,&nbsp;Sebastian Bürklein Prof. Dr. med. dent. ,&nbsp;Dag Harmsen Prof. Dr. med. ,&nbsp;Benjamin Ehmke Prof. Dr. med. dent.","doi":"10.1016/j.joen.2024.11.007","DOIUrl":"10.1016/j.joen.2024.11.007","url":null,"abstract":"<div><h3>Introduction</h3><div>The aim was to evaluate the suitability of paper points or endodontic nickel-titanium files to sample microorganisms for <em>in vivo</em> investigation of endodontic microbiota by 16S ribosomal DNA (rDNA) sequencing.</div></div><div><h3>Methods</h3><div>Forty-five patients presenting clinical and radiological signs of apical periodontitis were recruited for sampling, giving their written informed consent. Glide paths were assessed using C-Pilot Files and K-Files under electronic root canal length control under aseptic conditions. Microbial samples were taken from 84 root canals in duplicate, the first sample with a sterile paper point (size 15), the second with a sterile file (size 20/.06). After DNA extraction, the hypervariable region V4 of the bacterial 16 S rRNA gene was amplified and sequenced (Illumina MiSeq). Sequencing data were trimmed with Cutadapt and exact amplicon sequence variants generated by DADA2. Taxonomy was assigned based on the Human Oral Microbiome Database (eHOMD). Statistical analysis of diversity parameters comprised Wilcoxon signed-rank tests and permutational analysis of variance (PERMANOVA). Compositional differences were evaluated by differential abundance analysis (DESeq2). Microbial contamination during the sampling process and analysis were evaluated.</div></div><div><h3>Results</h3><div>Concerning alpha diversity, richness and dissimilarity differed nonsignificantly between paper point and instrument samples (<em>P</em> &gt; .05), whereas a significant difference was observed in the Shannon index (<em>P</em> &lt; .05). Regarding beta diversity, paper point and instrument samples presented with similar microbial community compositions (<em>P</em> = 1.0, PERMANOVA). Paper point controls contained significantly higher proportions of Pseudomonadales (<em>P</em> &lt; .05).</div></div><div><h3>Conclusions</h3><div>Paper point and endodontic instrument sampling generate valid specimens for 16S rDNA community profiling. Endodontic instrument sampling is easier to execute and, therefore, could be the technique of choice.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 2","pages":"Pages 164-171"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Management of Orofacial Trauma Including Multiple Avulsions, Lateral Luxation, and Alveolar Fracture: A Case Report with 7-Years of Follow-Up","authors":"Filipe Colombo Vitali DDS, MSc, PhD,&nbsp;Gabriela Ribeiro Sumar DDS,&nbsp;Julia Menezes Savaris DDS, MSc,&nbsp;Charles Marin DDS, MSc, PhD,&nbsp;Lucas da Fonseca Roberti Garcia DDS, MSc, PhD,&nbsp;Cleonice da Silveira Teixeira DDS, MSc, PhD","doi":"10.1016/j.joen.2024.11.015","DOIUrl":"10.1016/j.joen.2024.11.015","url":null,"abstract":"<div><div>The occurrence of different types of trauma concomitantly can complicate the establishment of the most appropriate treatment. This report describes the clinical management of orofacial trauma including multiple avulsions, lateral luxation, alveolar fracture, and their sequelae after 7 years of follow-up. A 19-year-old woman presented to emergency care 4 hours after an accidental fall that resulted in the avulsion of teeth #8, #9, and #25; a complicated crown fracture and lateral luxation of tooth #7; an uncomplicated crown fracture of tooth #10; and a fracture of the maxillary alveolar bone. Only teeth #8 and #9 were located by the patient. The immediate treatment included surgical reduction and extrusion of the lateral luxation, delayed replantation of the avulsed central incisors, nonrigid splint stabilization, and suturing of gingival lacerations. Subsequent visits involved removal of the splint, root canal treatment of the maxillary incisors, implant placement to replace the avulsed mandibular incisor, and esthetic rehabilitation with composite resin. At the 5-year follow-up, cone beam computed tomography revealed advanced surface root resorption in the replanted incisors. At the 7-year follow-up, an aggravation of inflammatory resorption in the replanted incisors was observed. This report demonstrates that adequate knowledge of trauma management by clinicians is essential for the effective management of more complex cases. It also highlights the importance of long-term follow-up in cases of avulsion and replantation due to potential long-term sequelae.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 2","pages":"Pages 213-221.e1"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological Markers in the Gingival Crevicular Fluid Associated with External Invasive Resorption: A Split-Mouth Cross-Sectional Study","authors":"Matthew Malek DDS,&nbsp;Laura Steff DDS,&nbsp;Nikita Vakil DDS,&nbsp;Rathimala Jothilingam DDS,&nbsp;Sunna Huh DDS,&nbsp;Tiffany Chien DDS,&nbsp;Rachel Halpern DDS,&nbsp;Nihan Gencerliler MPH ,&nbsp;Ozge Erdoğan DDS, DMSc ,&nbsp;Kamolthip Songtrakul DDS, MS","doi":"10.1016/j.joen.2024.11.013","DOIUrl":"10.1016/j.joen.2024.11.013","url":null,"abstract":"<div><h3>Introduction</h3><div>This study aimed to investigate the levels of interleukin-1 receptor antagonist (IL-1RA), receptor activator of nuclear factor-κB ligand (RANKL), osteoprotegerin (OPG), interleukin-1β, osteopontin, and tissue necrotizing factor-α in teeth with external invasive resorption (EIR) in comparison to the contralateral healthy tooth of the same patient.</div></div><div><h3>Methods</h3><div>Twenty-nine patients with at least one tooth with EIR and a healthy tooth on the contralateral side (33 paired teeth) were included. Data on patient demographics and medical and dental history were collected. Gingival crevicular fluid was collected from the subject and control teeth. Clinical, radiographical, and cone-beam computed tomography examinations were performed. Wilcoxon signed-rank test was used to compare biomarker concentrations, RANKL/OPG, and IL-1RA/interleukin-1β ratios in the diseased and healthy teeth. Teeth pairs were grouped based on Heithersay and Patel's classification, and the differences in biomarker concentrations between diseased and healthy teeth were compared using a Kruskal–Wallis test.</div></div><div><h3>Results</h3><div>Teeth with EIR had significantly lower concentrations of IL-1RA and OPG compared to their respective healthy control teeth (<em>P</em> &lt; .05). The RANKL/OPG ratio in teeth with EIR was significantly higher than in their paired healthy teeth (<em>P</em> &lt; .05). A history of herpes zoster infection was associated with a higher IL-1RA concentration and RANKL/OPG ratio (<em>P</em> &lt; .05). Orthodontic treatment was significantly associated with lower OPG concentration (<em>P</em> &lt; .05).</div></div><div><h3>Conclusions</h3><div>There is a significant association between EIR and specific biological markers. A history of orthodontic treatment and herpes zoster infection are significantly associated with altered levels of biomarkers in the gingival crevicular fluid.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 2","pages":"Pages 150-156.e1"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of pH on the Piezoelectric Properties of Dentin in Root Canal Treated Teeth: Implications for Dental Materials and Oral Health","authors":"Mohammad Ali Saghiri BS, MS, DEng, PhD ,&nbsp;Mina Shekarian DDS ,&nbsp;Fatereh Samadi DDS ,&nbsp;David S. Briss DMD, FRCD(C) ,&nbsp;Salvatore Napoli DDS, MD, FACS ,&nbsp;Michael Conte DMD, MPH","doi":"10.1016/j.joen.2024.11.001","DOIUrl":"10.1016/j.joen.2024.11.001","url":null,"abstract":"<div><h3>Introduction</h3><div>This study aimed to evaluate the impact of pH on the dentin piezoelectric response in root canal treated teeth.</div></div><div><h3>Materials and Methods</h3><div>Thirty extracted mandibular canines were selected by simple random sampling and based on specific inclusion criteria. The middle third of each root was cut into cubic specimens of approximately 2 mm × 2 mm x 4 mm. The prepared samples were soaked in 17% EDTA and 1% sodium hypochlorite for 1 minute. The sections were rinsed in distilled water, dried immediately, and then randomly allocated into 3 groups of 10 each. Samples in each group were soaked in one of 3 solutions with pH 4.4, 7.4, or 10.4 for 5 min. A compression load was applied by a universal mechanical testing machine. The 8000-8-SM scanner detected the potential generated.</div></div><div><h3>Results</h3><div>No significant differences were observed among the groups when the force was applied vertical to the dentinal tubules (<em>P</em> &gt; .05). However, all subgroups indicated significant differences when the force was applied along the dentinal tubules (<em>P</em> &lt; .05). When the force was applied along to the tubules, the mean dentin piezoelectricity was higher in the alkaline, neutral, and acidic groups, respectively.</div></div><div><h3>Conclusion</h3><div>This study demonstrated that the piezoelectric properties of dentin in root canal treated teeth can be influenced by variations in pH, depending on the direction of force applied to the dentinal tubules.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 2","pages":"Pages 195-199"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142681721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Relationships Between Circulating Interleukins and Pulp and Periapical Diseases: A Bidirectional Mendelian Randomization Study","authors":"Yuqiang Wang DDS, MSc,&nbsp;Jiakang Zhu DDS, MSc, PhD,&nbsp;Yueli Zhou DDS, MSc, PhD,&nbsp;Ying Tang DDS, MSc, PhD,&nbsp;Cui Huang DDS, MSc, PhD","doi":"10.1016/j.joen.2024.11.010","DOIUrl":"10.1016/j.joen.2024.11.010","url":null,"abstract":"<div><h3>Introduction</h3><div>The aim of this study was to comprehensively evaluate the links of genetic predisposition among 13 common circulating interleukins (ILs) and endodontic diseases by using a 2-sample Mendelian randomization method.</div></div><div><h3>Methods</h3><div>Single nucleotide polymorphisms were chosen as instrumental variables from summary data of genome-wide association studies (GWASs), using the inverse-variance–weighted method as the primary analysis. In addition, a variety of sensitivity analyses was conducted to evaluate the resilience of the primary outcomes and identify any inherent pleiotropic effects.</div></div><div><h3>Results</h3><div>After multiple comparison corrections, 4 circulating ILs were genetically predicted to significantly play a role in endodontic diseases. Among these, circulating IL-17 (odds ratio [OR]_IVW, 1.33; 95% confidence interval [CI], 1.15–1.54; <em>P</em> &lt; 3.85 × 10⁻³) were significantly identified as potential risk-increasing factors toward pulp and periapical diseases, and circulating IL-16 (OR_IVW, 0.89; 95% CI, 0.83 to 0.94; <em>P</em> &lt; 3.85 × 10⁻³) may exert protective effects on the development of periapical abscess. In the reverse analyses, null significantly association was found between genetic liability to endodontics disorders and the corresponding circulating inflammatory biomarkers. Overall, sensitivity analyses were consistent with the estimates direction of primary analyses results, supporting the reliability of findings.</div></div><div><h3>Conclusions</h3><div>Given the unavoidable limitations presented in this study, our findings provided significant evidence to support the identification of elevated IL-16 levels as a potential risk-mitigating factor, whereas elevated IL-17 levels exhibit potentially deleterious effects on endodontic disorders. Future validation is warranted to examine the conclusions of our study and to evaluate the potential application of these circulating ILs as lifestyle or pharmacological targets for oral health care.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 2","pages":"Pages 132-139"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}