Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie最新文献_第9页

Comparison of five routine methods with the candidate reference method for the determination of bilirubin in neonatal serum. 5种常规方法与候选参考方法测定新生儿血清胆红素的比较。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-04-01 DOI: 10.1515/cclm.1990.28.4.203

H Schlebusch, K Axer, C Schneider, N Liappis, G Röhle

{"title":"Comparison of five routine methods with the candidate reference method for the determination of bilirubin in neonatal serum.","authors":"H Schlebusch, K Axer, C Schneider, N Liappis, G Röhle","doi":"10.1515/cclm.1990.28.4.203","DOIUrl":"https://doi.org/10.1515/cclm.1990.28.4.203","url":null,"abstract":"Using five routine methods and the candidate reference method of Doumas (Clin. Chem. 31, 1779-1789 (1985)), total bilirubin was determined in 77 neonatal serum samples (concentration range 63-444 mumol/l, average value 227 mumol/l). Four of the routine methods (Jendrassik & Grof's (Biochem. Z. 297, 81-89 (1938)) method, Hertz's (Scand. J. Clin. Lab. Invest. 33, 215-230 (1974)) method, the bilirubinometer procedure, and the method employing 2,5-dichlorophenyldiazonium (Scand. J. Clin. Lab. Invest. 29, Suppl. 126, Abstr. 11. 12. (1972]) gave values that were generally higher than those of the reference method. In contrast, the results from Vink's (Clin. Chem. 34, 67-70 (1988] direct spectrophotometric method differed only negligibly from those of the reference method. The accuracy of Jendrassik & Grof's method, and to a limited extent that of the 2,5-dichlorophenyldiazonium method, can be improved by redetermination of the molar absorption coefficient, or by using a standard containing a matrix of human albumin, with an assigned value determined by the reference method. It was found that Hertz's direct spectrophotometric method can be replaced by that of Vink. The accuracy of the bilirubinometer results could be improved only by using calibrators with assigned values specific for the bilimeter, or by calibration with a serum pool.","PeriodicalId":15649,"journal":{"name":"Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"28 4","pages":"203-10"},"PeriodicalIF":0.0,"publicationDate":"1990-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1990.28.4.203","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13507443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Enzyme immunoassay of human cholinesterase (EC 3.1.1.8). Comparison of immunoreactive substance concentration with catalytic activity concentration in randomly selected serum samples from healthy individuals. 人胆碱酯酶(EC 3.1.1.8)的酶免疫测定。随机抽取健康人群血清样本中免疫反应物质浓度与催化活性浓度的比较。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-04-01 DOI: 10.1515/cclm.1990.28.4.221

A Brock, V Mortensen, A G Rasmussen Loft, B Nørgaard-Pedersen

引用次数: 11

Multicentre evaluation of the Boehringer Mannheim/Hitachi 717 analysis system. 勃林格曼海姆/日立717分析系统的多中心评估。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-04-01

H Baadenhuijsen, P M Bayer, H Keller, M Knedel, N Montalbetti, S Brenna, L Prencipe, A Vassault, M Bailly, H T Phung

引用次数: 0

Parathyrin assay. An analytical evaluation of two commercial Immuno Radiometric Assay kits. Parathyrin化验。两种商业免疫放射测定试剂盒的分析评价。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-04-01 DOI: 10.1515/cclm.1990.28.4.211

D R Pledger, C Carr, T I Sims, K R Johnson

{"title":"Parathyrin assay. An analytical evaluation of two commercial Immuno Radiometric Assay kits.","authors":"D R Pledger, C Carr, T I Sims, K R Johnson","doi":"10.1515/cclm.1990.28.4.211","DOIUrl":"https://doi.org/10.1515/cclm.1990.28.4.211","url":null,"abstract":"We have performed a comparative evaluation of two Immuno Radiometric Assay (IRMA) kits for parathyrin against an existing Radioimmuno Assay (RIA) technique for the measurement of intact parathyrin. The analytical evaluation which was performed in line with the ECCLS recommended kit evaluation protocol showed a marginal improvement in precision with the new assays. There was a substantial improvement in the theoretical limit of detection utilising the IRMA kits although it may prove difficult to realize this improvement in practice with individual samples because of differing protein matrices. The evaluation also demonstrated the degree of parallelism and range of linearity of both kits as well as inaccuracies when compared with the International Reference Preparation for parathyrin (IRP 79/500) as the accepted standard. The demonstration of lack of agreement between measured and kit assigned results for standards when cross-over studies between kits were performed may highlight a possible contributing factor to inaccuracy. Alternatively there may be a difference of antisera avidity within the kits for intact parathyrin. Whilst minor differences in sample stability were demonstrated between the kits, the sample stability was much improved compared to that for intact parathyrin measurement by RIA. The correlation studies showed a degree of correlation consistent with other comparisons similarly performed.","PeriodicalId":15649,"journal":{"name":"Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"28 4","pages":"211-6"},"PeriodicalIF":0.0,"publicationDate":"1990-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1990.28.4.211","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13507444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Standardisation of cholesterol determination. 胆固醇测定标准化。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-04-01

G J Boerma

引用次数: 0

A new development in haematological cell counting: the Sysmex NE-8000, automaton for cell count and physical five-part leukocyte differentiation. 血液学细胞计数的新进展:Sysmex NE-8000，细胞计数和物理五部分白细胞分化的自动性。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-04-01 DOI: 10.1515/cclm.1990.28.4.233

J W van Wersch, C Bank

{"title":"A new development in haematological cell counting: the Sysmex NE-8000, automaton for cell count and physical five-part leukocyte differentiation.","authors":"J W van Wersch, C Bank","doi":"10.1515/cclm.1990.28.4.233","DOIUrl":"https://doi.org/10.1515/cclm.1990.28.4.233","url":null,"abstract":"We compared the results of the Sysmex NE-8000 with those of the Sysmex E-4000 and the Technicon H-6000. Both for the whole blood cell count and the leukocyte differentiation we obtained intra- and inter-assay coefficients of variation fully comparable with those for other cell counters. The carry-over between samples was negligibly small. The linearity range was broad, which implies a universal application in routine haematological analysis. The numerical results from the Sysmex NE-8000 and the Technicon H-6000 corresponded closely, with exception of the mean corpuscular volume and the haematocrit, whereas these parameters showed excellent agreement between the NE-8000 and the E-4000. In the differentiation of leukocytes, the NE-8000 and H-6000 showed good correlation and conformity for lymphocytes, neutrophils and eosinophils; for monocytes and basophils the conformity was poor. We also found a good correlation between the NE-8000 and E-4000 for lymphocytes and neutrophils, whereas the correlation was less for the middle cell ratio and the sum of monocytes, eosinophils and basophils. Furthermore, we compared Receiver Operating Characteristic curves for the different leukocyte subtypes on the NE-8000, the H-6000 and the E-4000, and investigated the flagging frequency in inpatients, outpatients and blood donors.","PeriodicalId":15649,"journal":{"name":"Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"28 4","pages":"233-40"},"PeriodicalIF":0.0,"publicationDate":"1990-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1990.28.4.233","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13507446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Serum phospholipase A and prognosis in intensive care patients. 重症监护患者血清磷脂酶A与预后。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-04-01

R Schaefer, M Teschner, W Penzel, W Habscheid, A Heidland, G E Hoffmann

引用次数: 0

The Reflotron Total Cholesterol assay in heparinized venous and capillary blood. 肝素化静脉血和毛细血管血Reflotron总胆固醇测定。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-04-01

G J Boerma, T L Liem, J A Jacobs-de Haas, F A de Jong-van Staveren, W Verbree, I Eman

{"title":"The Reflotron Total Cholesterol assay in heparinized venous and capillary blood.","authors":"G J Boerma, T L Liem, J A Jacobs-de Haas, F A de Jong-van Staveren, W Verbree, I Eman","doi":"","DOIUrl":"","url":null,"abstract":"We recently evaluated the Reflotron Total Cholesterol dry chemistry assay by examining the calibration and the accuracy of the assay in serum, whole venous EDTA-blood and capillary EDTA blood. We now describe a study on the analysis in heparinized venous and heparinized finger stick blood. Cholesterol assays in venous serum and plasma, and in finger capillary blood and plasma (all with heparin) were compared. Finger capillary blood was obtained in two ways: 1. in heparinized capillaries; 2. in a Becton Dickinson microtainer that could be centrifuged for separation of the plasma. Venous blood was obtained in plain tubes (for serum) and heparinized tubes to obtain venous whole blood and also venous plasma. We did not, on average, find large differences between the concentrations of cholesterol in the various materials. The regression equations for finger capillary whole blood and venous whole blood however show higher slopes than the others. Inaccuracy due to the sampling technique appears to be no greater for finger capillary samples than for venous samples.","PeriodicalId":15649,"journal":{"name":"Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"28 4","pages":"255-8"},"PeriodicalIF":0.0,"publicationDate":"1990-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13507449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The diagnostic value of neuron-specific enolase and carcino-embryonic antigen analyses in patients with carcinoma of the lung. 神经元特异性烯醇化酶和癌胚抗原分析对肺癌的诊断价值。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-04-01 DOI: 10.1515/cclm.1990.28.4.225

J P Wielders, C T Bartels, C M Bank, J C Meek, M P van Dieijen-Visser, P J Brombacher

{"title":"The diagnostic value of neuron-specific enolase and carcino-embryonic antigen analyses in patients with carcinoma of the lung.","authors":"J P Wielders, C T Bartels, C M Bank, J C Meek, M P van Dieijen-Visser, P J Brombacher","doi":"10.1515/cclm.1990.28.4.225","DOIUrl":"https://doi.org/10.1515/cclm.1990.28.4.225","url":null,"abstract":"Neuron-specific enolase and carcino-embryonic antigen were quantified simultaneously in sera of 135 patients attending the Department of Respiratory Diseases for diagnostic bronchoscopy. Fifteen small cell lung carcinomas, 24 non-small cell lung carcinomas and 96 benign pulmonary diseases were investigated. Lung biopsies or bronchial washings were obtained from about 75% of the patients, including all patients with neoplastic diseases. Serum neuron-specific enolase was measured by a recently introduced enzyme-immuno assay (WaKo NS-Enolase EIA-II testkit). The results obtained with this kit were similar to those based on RIA assays. Receiver Operating Characteristic curves (ROC curves) were constructed for comparison of the discriminating ability of neuron-specific enolase and carcino-embryonic antigen in small cell lung carcinomas and non-small cell lung carcinomas. For small cell lung carcinomas the sensitivity and the specificity of neuron-specific enolase (cutoff value: 10 micrograms/l) were 87% and 88%, respectively, and for carcino-embryonic antigen values 60% and 77% were obtained. There was no correlation between neuron-specific enolase and carcino-embryonic antigen in small cell lung carcinoma patients. The diagnostic value of neuron-specific enolase and carcino-embryonic antigen in non-small cell lung carcinomas is illustrated by sensitivities of 13% and 58%, respectively. An extensive literature survey is included to allow comparison with other studies. The use of ROC curves is recommended for the determination of optimal cutoff values for the assays employed.","PeriodicalId":15649,"journal":{"name":"Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"28 4","pages":"225-31"},"PeriodicalIF":0.0,"publicationDate":"1990-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1990.28.4.225","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13315773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Mutations of the mitochondrial DNA: the contribution of DNA techniques to the diagnosis of mitochondrial encephalomyopathies. 线粒体DNA的突变:DNA技术对线粒体脑肌病诊断的贡献。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-04-01 DOI: 10.1515/cclm.1990.28.4.241

K D Gerbitz, B Obermaier-Kusser, P Lestienne, S Zierz, J Müller-Höcker, D Pongratz, I Paetzke-Brunner, T Deufel

{"title":"Mutations of the mitochondrial DNA: the contribution of DNA techniques to the diagnosis of mitochondrial encephalomyopathies.","authors":"K D Gerbitz, B Obermaier-Kusser, P Lestienne, S Zierz, J Müller-Höcker, D Pongratz, I Paetzke-Brunner, T Deufel","doi":"10.1515/cclm.1990.28.4.241","DOIUrl":"https://doi.org/10.1515/cclm.1990.28.4.241","url":null,"abstract":"We performed restriction analysis and Southern blotting of the muscle mitochondrial DNA from 34 patients suffering from different myopathies. In 13/21 patients with chronic progressive external ophthalmoplegia the muscle mitochondrial DNA was shown to be heteroplasmic. Further mapping by use of several restriction enzymes yielded large deletions in muscles from 10/13 chronic progressive external ophthalmoplegia patients. Most of the deletions spanned large parts of the mitochondrial genome, leading to loss of mitochondrial genes encoding several subunits of the respiratory chain complexes I (NADH-dehydrogenase), IV (cytochrome c oxidase) and V (ATP-synthetase), as well as of several tRNAs. Comparison of the mapping data with the histochemical and biochemical results did not provide a clear correlation between the location of the mitochondrial genetic defects and the functional deficiencies of the affected respiratory chain complexes. In the majority of patients with chronic progressive external ophthalmoplegia, but without a family history of the disease, restriction analysis reveals large mutations of the mitochondrial genome, while other methods are necessary for the localization of defects in all cases with maternal transmission of the disease. The same holds true for all other kinds of mitochondrial myopathies based on defects within the nuclear DNA or on derangements of the \"cross-talk\" between the nuclear and the mitochondrial genomes.","PeriodicalId":15649,"journal":{"name":"Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"28 4","pages":"241-50"},"PeriodicalIF":0.0,"publicationDate":"1990-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1990.28.4.241","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13315774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13