Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie最新文献_第10页

A new chemiluminescence immunoassay for triiodothyronine and thyroxine: evaluation using quality control sera assayed in an interlaboratory survey. 一种新的化学发光免疫法测定三碘甲状腺原氨酸和甲状腺素:用实验室间调查的质控血清进行评价。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-04-01 DOI: 10.1515/cclm.1990.28.4.193

G C Zucchelli, A Pilo, S Masini, M R Chiesa, C Prontera

{"title":"A new chemiluminescence immunoassay for triiodothyronine and thyroxine: evaluation using quality control sera assayed in an interlaboratory survey.","authors":"G C Zucchelli, A Pilo, S Masini, M R Chiesa, C Prontera","doi":"10.1515/cclm.1990.28.4.193","DOIUrl":"https://doi.org/10.1515/cclm.1990.28.4.193","url":null,"abstract":"A recently developed chemiluminescence immunoassay system (LIA-mat) for triiodothyronine and thyroxine, set up by Byk-Sangtec Diagnostica (Dietzenbach, Germany), has been evaluated and compared with radioimmunoassays and with a chemiluminescence enhanced enzyme immunoassay (Amerlite), using control materials circulated in a national interlaboratory quality control, as well as patient sera. The LIA-mat assays are competitive methods which use coated monoclonal antibodies and triiodothyronine- or thyroxine-ABEI (aminobutylethylisoluminol) conjugate as tracers. The working range of LIA-mat T3 (computed from the within-assay precision profile) extended from 1.4 to 12.3 nmol/l; the between-assay precision was 8.1 - 19.3 CV%. Regression analysis of the LIA-mat T3 results (y) against the consensus means (x) of the participants in the national interlaboratory survey yielded: y = -0.14 + 1.05 x, r = 0.95. The working range of LIA-mat T4 extended from 33 to 515 nmol/l; the between-assay precision was 5.4 - 9.2 CV%. An excellent agreement was found between LIA-mat T4 results (y) and the consensus means (x) of the laboratories participating in the national interlaboratory survey (y = 3.79 + 1.02 x, r = 0.98).","PeriodicalId":15649,"journal":{"name":"Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"28 4","pages":"193-7"},"PeriodicalIF":0.0,"publicationDate":"1990-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1990.28.4.193","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13345840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Direct determination of chloramphenicol acetyltransferase (CAT) activity in homogenates of transfected mammalian cells by high performance liquid chromatography. 高效液相色谱法直接测定转染动物细胞匀浆中氯霉素乙酰转移酶(CAT)活性。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-04-01 DOI: 10.1515/cclm.1990.28.4.217

H Siegert, B Wittig, S Wölfl

引用次数: 0

The fructosamine assay in diagnosis and control of diabetes mellitus scientific evidence for its clinical usefulness? 果糖胺测定在糖尿病诊断和控制中的临床应用的科学依据?

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-03-01

J Windeler, J Köbberling

{"title":"The fructosamine assay in diagnosis and control of diabetes mellitus scientific evidence for its clinical usefulness?","authors":"J Windeler, J Köbberling","doi":"","DOIUrl":"","url":null,"abstract":"The fructosamine assay is about to be used for the control of diabetic patients and for the diagnosis of diabetes mellitus. An analysis of the original literature is presented to answer the question whether the evaluation of the test is methodologically reliable. Altogether 65 studies are evaluated. Thirty of these studies deal with technical aspects of the test (phase 1). A comparison of the distributions of test results for diabetic and non-diabetic patients is presented in 28 studies (phase 2). However, serious shortcomings in methodology and presentation are apparent. \"Significant\" differences and correlation coefficients are the basis for optimistic conclusions. Only 7 studies are identified where fundamental test parameters such as sensitivity and specificity are given, or can be calculated (phase 3). None of them achieves a satisfactory standard of methodology (selection of patients unclear, assessment made not mutually blind). There is no reliable phase-3-study for the metabolic control of diabetes mellitus. It can be concluded that the fructosamine test is not evaluated sufficiently to allow its routine clinical use. In fact, contrary to a widely quoted opinion, the results presented so far do not suggest a reliable test.","PeriodicalId":15649,"journal":{"name":"Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"28 3","pages":"129-38"},"PeriodicalIF":0.0,"publicationDate":"1990-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13293792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alveolar cell pattern and chemiluminescence response of blood neutrophils and alveolar macrophages in sheep after endotoxin injection. 内毒素注射后绵羊肺泡细胞形态及血液中性粒细胞和肺泡巨噬细胞的化学发光反应。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-03-01 DOI: 10.1515/cclm.1990.28.3.163

A Dwenger, G Regel, B Ellendorff, G Schweitzer, M Funck, H Limbrock, J A Sturm, H Tscherne

引用次数: 11

Transthyretin--an explanation of "anomalous" serum thyroid hormone values in severe illness? 促甲状腺素——重症患者血清甲状腺激素值“异常”的解释?

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-03-01 DOI: 10.1515/cclm.1990.28.3.155

J Ramaker, W G Wood

{"title":"Transthyretin--an explanation of \"anomalous\" serum thyroid hormone values in severe illness?","authors":"J Ramaker, W G Wood","doi":"10.1515/cclm.1990.28.3.155","DOIUrl":"https://doi.org/10.1515/cclm.1990.28.3.155","url":null,"abstract":"The following serum analytes were measured in 464 patients with defined carcinomas and other tumours as well as those with chronic obstructive lung disease and under regular haemodialysis, and in 261 healthy controls: thyrotropin (TSH), thyroxine (T4), triiodothyronine (T3), free thyroxine (fT4), thyroxine binding globulin (TBG) and transthyretin (TTR). The following ratios were constructed: fT4 x TTR, defined as the thyroxine availability index fT4/TTR, defined as the thyroid hormone compensation index 100 x T3/TBG as the free T3 index (fT3I) and fT3I x TTR, defined as the triiodothyronine availability index. Significantly elevated thyrotropin values (p = 0.05) were only found in patients with breast cancer when compared with age matched controls, although elevated T4 and fT4 values were found in all experimental groups except the haemodialysis patients (p less than 0.01). The thyroxine availability index and triiodothyronine availability index values were not significantly different from the age matched controls (greater than 60 a) in the cancer groups, showing that the transthyretin concentrations compensated for changes in fT4 or vice versa. These findings are reflected in the euthyroid thyrotropin values. The T4 and fT4 values in the dialysis patient group were significantly lower than in the age matched controls (p less than 0.01), while the transthyretin values were significantly higher (p less than 0.01), which accounted for the normal thyroxine availability index and euthyroid thyrotropin values.","PeriodicalId":15649,"journal":{"name":"Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"28 3","pages":"155-61"},"PeriodicalIF":0.0,"publicationDate":"1990-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1990.28.3.155","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13265107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Determination of cathepsins B and H in sera and synovial fluids of patients with different joint diseases. 不同关节疾病患者血清及滑液组织蛋白酶B、H的测定。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-03-01 DOI: 10.1515/cclm.1990.28.3.149

D Gabrijelcic, A Annan-Prah, B Rodic, B Rozman, V Cotic, V Turk

{"title":"Determination of cathepsins B and H in sera and synovial fluids of patients with different joint diseases.","authors":"D Gabrijelcic, A Annan-Prah, B Rodic, B Rozman, V Cotic, V Turk","doi":"10.1515/cclm.1990.28.3.149","DOIUrl":"https://doi.org/10.1515/cclm.1990.28.3.149","url":null,"abstract":"Synovial fluids and sera of patients with inflammatory and metabolic joint diseases contain different cysteine proteinases. The quantities of cathepsins B and H were determined by newly developed specific enzyme-linked immunoassay tests (ELISA), with detection limits of 0.5 microgram/l for cathepsin B and 3 micrograms/l for cathepsin H. The values of cathepsin B in normal sera ranged from 0.6 microgram/l to 2 micrograms/l, whereas in sera of patients with joint diseases they ranged from 1.7 micrograms/l to 18 micrograms/l. Cathepsin H was not found in sera (values below 3 micrograms/l), but was measurable in patients' synovial fluids. Patients with rheumatoid arthritis have on average the highest values of cathepsin B in synovial fluids, whereas patients with undifferentiated arthritis have the highest values of cathepsin H. The results show that cathepsins B and H are present in arthritic synovial fluids, where they may be implicated in destructive processes. There is yet no clear correlation between the quantity of each cathepsin released in synovia and the clinical diagnosis or the stage of the disease.","PeriodicalId":15649,"journal":{"name":"Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"28 3","pages":"149-53"},"PeriodicalIF":0.0,"publicationDate":"1990-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1990.28.3.149","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13479029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 46

Unforeseen effect of thyroxine binding globulin when using the microencapsulated antibody method to determine free thyroxine (FT4): misleading results due to circulating unsaturated thyroxine binding globulin. 使用微囊化抗体法测定游离甲状腺素(FT4)时，甲状腺素结合球蛋白的不可预见的影响:由于循环中不饱和甲状腺素结合球蛋白的存在，结果会产生误导。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-03-01 DOI: 10.1515/cclm.1990.28.3.175

T Hashimoto, K Ishibashi, M Nagahara, F Matsubara

{"title":"Unforeseen effect of thyroxine binding globulin when using the microencapsulated antibody method to determine free thyroxine (FT4): misleading results due to circulating unsaturated thyroxine binding globulin.","authors":"T Hashimoto, K Ishibashi, M Nagahara, F Matsubara","doi":"10.1515/cclm.1990.28.3.175","DOIUrl":"https://doi.org/10.1515/cclm.1990.28.3.175","url":null,"abstract":"The effect of varying concentrations (0-52 mg/l) of purified thyroxine binding globulin (TBG) on the microencapsulated antibody method for free thyroxine was investigated. The results demonstrated that the free thyroxine values were strongly influenced by the concentration of thyroxine binding globulin in the samples. The standard curve could no longer be distinguished at a concentration of purified thyroxine binding globulin of 52 mg/l. In the clinical application, we observed that the values obtained using the microencapsulated antibody method were significantly higher than the expected values in patients receiving triiodothyronine treatment after total thyroidectomy (theoretically nil) and in patients with untreated primary hypothyroidism with negligible thyroxine (less than 12.9 nmol/l). These false positive values are considered to be due to the methodological problem mentioned above, i.e. the microcapsule membrane is not efficient and therefore must be improved. Consequently, any data based on this method should be interpreted with caution.","PeriodicalId":15649,"journal":{"name":"Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"28 3","pages":"175-9"},"PeriodicalIF":0.0,"publicationDate":"1990-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1990.28.3.175","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13265108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Adaptation and evaluation of a chromogenic test procedure for fibrin monomers on a centrifugal analyzer. 在离心分析仪上对纤维蛋白单体显色测试程序的适应和评价。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-03-01 DOI: 10.1515/cclm.1990.28.3.169

J W van Wersch

引用次数: 4

Who defines the medical requirements of analytical tests? 谁定义分析测试的医疗要求?

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-03-01

W G Guder, E Schleicher

引用次数: 0

Immunoassay by particle counting for coagulation testing: application to the determination of protein C. 凝血试验用颗粒计数免疫测定:用于蛋白C的测定。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-03-01

S Poncelet, E Lavenne, J N Limet, D Collet-Cassart

引用次数: 0