人胆碱酯酶(EC 3.1.1.8)的酶免疫测定。随机抽取健康人群血清样本中免疫反应物质浓度与催化活性浓度的比较。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-04-01 DOI:10.1515/cclm.1990.28.4.221

A Brock, V Mortensen, A G Rasmussen Loft, B Nørgaard-Pedersen

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引用次数: 11

摘要

我们开发了一种酶免疫测定法(ELISA)，用于测定天然血浆或血清样品中的血浆胆碱酯酶物质浓度。ELISA检测基于多克隆(兔)抗人胆碱酯酶和高度特异性的单克隆(小鼠)抗体，以市售的针对小鼠免疫球蛋白的过氧化物酶偶联(兔)抗体为信号载体。随机抽取33例健康人血清样品，检测到的血清胆碱酯酶物质浓度(均值4.51 mg/l，标准差0.90 mg/l)与相应的催化活性浓度呈密切线性相关。

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Enzyme immunoassay of human cholinesterase (EC 3.1.1.8). Comparison of immunoreactive substance concentration with catalytic activity concentration in randomly selected serum samples from healthy individuals.

We developed an enzyme immunoassay (ELISA) for quantitation of plasma cholinesterase substance concentrations in native plasma or serum samples. The ELISA assay is based on polyclonal (rabbit) antihuman cholinesterase and a highly specific monoclonal (mouse) antibody, with a commercially available peroxidase-conjugated (rabbit) antibody directed against mouse immunoglobulins as the signal carrier. The detected serum cholinesterase substance concentrations (mean: 4.51 mg/l, SD: 0.90 mg/l) in randomly selected serum samples from 33 healthy individuals were closely and linearly related to the corresponding catalytic activity concentrations.

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Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie

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