Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie最新文献_第8页

Use of airless sealed plastic tubes in interlaboratory quality control of various protein profiles. 无气密封塑料管在各种蛋白质谱的实验室间质量控制中的应用。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-05-01

B Dingeon, L Monnet, R Later

引用次数: 0

Determination of L-carnitine in biological fluids and tissues. 生物体液和组织中左旋肉碱的测定。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-05-01

T Deufel

{"title":"Determination of L-carnitine in biological fluids and tissues.","authors":"T Deufel","doi":"","DOIUrl":"","url":null,"abstract":"In most biological materials, free L-carnitine is present together with short-chain and long-chain carnitine esters. These are differentiated mainly according to their solubility in aqueous solvents. A standardized extraction procedure is therefore essential for reproducible estimations of carnitine content. Assays of L-carnitine are based on the reaction of L-carnitine with acetyl CoA with formation of acetyl L-carnitine and free CoASH, catalysed by carnitine acetyl transferase (EC 2.3.1.7). The two main principles employed to monitor this reaction are a) measurement of the incorporation of radio-labelled acetyl groups derived from acetyl CoA into acetyl carnitine, and b) photometric determination of free CoASH formed in the reaction, using thiol-group colour reagents or an enzymatic reaction. To avoid the background due to thiol-compounds in the sample, we suggest the introduction of an oxidation step with hydrogen peroxide, which is then removed with catalase. Using this method, we have established reference ranges for total acid-soluble L-carnitine and free L-carnitine in serum (men: 44.2-79.3, and 34.8-69.5, women: 28.1-66.4 and 19.3-53.9 mumol/l, resp.), skeletal muscle (adults: 21.0-23.1, and 19.5-35.1, children: 16.1-39.0 and 12.1-25.5 mumol/g non-collagen protein, resp.), and urine. The concentration of long-chain acyl L-carnitine in serum is 2.0-4.0 mumol/l. Carnitine levels in serum, tissues and urine are age-dependent with lower levels in newborn children. The ratio of short-chain acyl carnitine to free carnitine is mainly a reflection of hepatic acetyl CoA production.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":15649,"journal":{"name":"Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"28 5","pages":"307-11"},"PeriodicalIF":0.0,"publicationDate":"1990-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13530681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of carnitine acyltransferase inhibitors on the performance and metabolism of rat cardiac muscle. 肉碱酰基转移酶抑制剂对大鼠心肌运动性能和代谢的影响。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-05-01

H Reinauer, M Adrian, P Rösen, F J Schmitz

{"title":"Influence of carnitine acyltransferase inhibitors on the performance and metabolism of rat cardiac muscle.","authors":"H Reinauer, M Adrian, P Rösen, F J Schmitz","doi":"","DOIUrl":"","url":null,"abstract":"The effects of carnitine palmitoyl transferase I inhibitors were studied in isolated perfused rat heart and in in vivo studies with normal and diabetic rats. In isolated perfused rat hearts of acutely diabetic and Zucker rats, clomoxir (sodium 2[5(4-chlorophenyl)pentyl]oxirane-2-carboxylate) inhibited the oxidation rate of endogenous fatty acids and increased the oxidation rate of glucose. Etomoxir, an analogue of clomoxir, was used in the in vivo studies with normal and chronic diabetic rats. Etomoxir (18 mg/kg) was given daily for 6 days by intraperitoneal injection. This carnitine palmitoyl transferase inhibitor significantly ameliorated the decreased heart performance in diabetic rats. The concentrations of glucose, glycerol, triacylglycerol, cholesterol, and phospholipids in serum were lower compared with untreated diabetic animals. On the other hand, the lipid and the carnitine content of heart and liver increased in the etomoxir-treated rats. Carnitine palmitoyl transferase inhibitors have clear antidiabetic effects, but before using as an oral antidiabetic drug, the long-term changes of the lipid and carnitine metabolism should be evaluated.","PeriodicalId":15649,"journal":{"name":"Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"28 5","pages":"335-9"},"PeriodicalIF":0.0,"publicationDate":"1990-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13528337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between intraindividual variation of the saliva/plasma- and of the arteriovenous concentration ratio as demonstrated by the administration of caffeine. 唾液/血浆和动静脉浓度比的个体变异之间的关系。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-05-01

R Haeckel

引用次数: 0

Carnitine: biochemical, analytical, experimental and clinical aspects. Workshop Conference of the Austrian and German Societies for Clinical Chemistry. Vienna, December 4-5, 1988. Proceedings. 肉毒碱:生化、分析、实验和临床方面。奥地利和德国临床化学学会研讨会。维也纳，1988年12月4日至5日。程序。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-05-01

引用次数: 0

Immunological determination of porphobilinogen deaminase as a diagnostic measure in acute intermittent porphyria. 急性间歇性卟啉症的免疫学诊断方法卟啉原脱氨酶的测定。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-05-01

L Lannfelt

{"title":"Immunological determination of porphobilinogen deaminase as a diagnostic measure in acute intermittent porphyria.","authors":"L Lannfelt","doi":"","DOIUrl":"","url":null,"abstract":"Manifest disease symptoms of acute intermittent porphyria may be provoked by several external factors. Latent gene carriers should be identified at an early stage and informed about preventive measures. Porphobilinogen deaminase activity in red blood cells may be used as one indicator of the carrier state. However, there is an overlap of enzyme activity between healthy controls and carriers of the trait. Thus latent gene carriers cannot always be identified. In the present study a recently reported enzyme-linked immunosorbent assay (ELISA) was used to quantify the concentration of the enzyme porphobilinogen deaminase in erythrocytes in 845 individuals belonging to families with acute intermittent porphyria. Using previous available diagnostic methods 417 of them had been diagnosed as gene carriers, 339 as non-carriers, and 89 were of \"uncertain\" classification. Of those with \"uncertain\" diagnosis, 19 had a decreased concentration of porphobilinogen deaminase and could thus be diagnosed as gene carriers. However, 70 cases of the 89 were still \"uncertain\", which underlines the need for further improvement of the diagnostic methods.","PeriodicalId":15649,"journal":{"name":"Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"28 5","pages":"273-8"},"PeriodicalIF":0.0,"publicationDate":"1990-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13530676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stimulation of glucose metabolism in human blood cells by inhibitors of carnitine-dependent fatty acid transport. 抑制肉毒碱依赖性脂肪酸运输对人血细胞葡萄糖代谢的刺激。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-05-01

R Haeckel, D Colic, L Binder, M Oellerich

{"title":"Stimulation of glucose metabolism in human blood cells by inhibitors of carnitine-dependent fatty acid transport.","authors":"R Haeckel, D Colic, L Binder, M Oellerich","doi":"","DOIUrl":"","url":null,"abstract":"According to a well accepted hypothesis, increased fatty acid oxidation can lead to hyperglycaemia by stimulating gluconeogenesis and reducing glycolysis. Therefore, inhibitors of fatty acid metabolism should cause hypoglycaemia by inhibiting gluconeogenesis and activating glycolysis. Various substances were tested to validate this hypothesis with regard to glucose oxidation in human mononuclear leukocytes and thrombocytes. 2-(3-Methyl-cinnamyl-hydrazono)-propionate, an inhibitor of the carnitine acyltransfer system was found to cause hypoglycaemia in whole animals and to inhibit gluconeogensis in the perfused guinea pig liver, while the acetyl-CoA/CoASH ratio was decreased. This substance stimulated the metabolism of glucose to CO2 in human mononuclear leukocytes and especially in platelets. This effect could be potentiated if concanavalin A and 2-(3-methyl-cinnamyl-hydrazono)-propionate were applied simultaneously. Under these conditions, however, fatty acid oxidation was no longer inhibited. From these results, it can be concluded that the activation of glucose oxidation by 2-(3-methyl-cinnamyl-hydrazono)-propionate is independent of its effect on fatty acid metabolism. Other inhibitors of fatty acid metabolism which were also investigated behaved similarly.","PeriodicalId":15649,"journal":{"name":"Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"28 5","pages":"329-33"},"PeriodicalIF":0.0,"publicationDate":"1990-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13528336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of changes in sodium balance on prostaglandin synthesis and prostaglandin E2 9-ketoreductase activity in the rat kidney. 钠平衡变化对大鼠肾脏前列腺素合成及前列腺素E2 9-酮还原酶活性的影响。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-04-01 DOI: 10.1515/cclm.1990.28.4.199

M Rathaus, E Podjarny, J Shapira, J Bernheim, M M Werber

{"title":"Effects of changes in sodium balance on prostaglandin synthesis and prostaglandin E2 9-ketoreductase activity in the rat kidney.","authors":"M Rathaus, E Podjarny, J Shapira, J Bernheim, M M Werber","doi":"10.1515/cclm.1990.28.4.199","DOIUrl":"https://doi.org/10.1515/cclm.1990.28.4.199","url":null,"abstract":"The in vitro synthesis of prostaglandins E2 and F2 alpha by renal cortex, medulla and papilla was measured in normal rats and in rats receiving either a low or a high sodium intake for 14 days. The production of both prostaglandins was unchanged in the cortex. In the medulla, both low and high sodium intakes led to a similar decrease in prostaglandin E2 synthesis in vitro, but prostaglandin F2 alpha synthesis was unchanged. In the papilla, a low sodium intake increased prostaglandin E2 synthesis. The activity of prostaglandin E2 9-ketoreductase, a cytosolic enzyme catalysing the conversion of prostaglandin E2 to prostaglandin F2 alpha, was unchanged in cortical preparations. In medullary slices, prostaglandin E2 9-ketoreductase activity was decreased by both sodium depletion and loading. In the papilla, prostaglandin E2 9-ketoreductase activity was slightly decreased by sodium loading and increased with sodium depletion. These results obtained in the rat are at variance with findings in the rabbit. The role played by prostaglandin E2 9-ketoreductase in the regulation of prostaglandin biosynthesis during changes of sodium balance remains controversial.","PeriodicalId":15649,"journal":{"name":"Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"28 4","pages":"199-202"},"PeriodicalIF":0.0,"publicationDate":"1990-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1990.28.4.199","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13507516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Serum reference values of the Cynomolgus monkey, a model for the study of atherosclerosis. 研究动脉粥样硬化模型食蟹猴的血清参考值。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-04-01

A Aouidet, H Bouissou, F de La Farge, P Valdiguié

引用次数: 0

Clinical chemistry in the European community. Development of international regulations on education, recognition and free exchange of professionals. European Communities Clinical Chemistry Committee. 欧洲共同体的临床化学。制定关于专业人员的教育、承认和自由交流的国际条例。欧洲共同体临床化学委员会。

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie Pub Date : 1990-04-01

P J Brombacher, J Breuer, G T Sanders

引用次数: 0