Journal of Diabetes and Metabolic Disorders最新文献

{"title":"Bioinformatics-led identification of pathophysiological hallmark genes in diabesotension via graph clustering method.","authors":"Muhammad-Redha Abdullah-Zawawi, Muhammad Irfan Abdul Jalal, Nor Afiqah-Aleng, Shah-Jahan Kamal-Chinakarppen, Nur Alyaa Afifah Md Shahri, Siti Aishah Sulaiman, Siok Fong Chin, Zeti-Azura Mohamed-Hussein, Rahman Jamal, Nor Azian Abdul Murad","doi":"10.1007/s40200-025-01659-9","DOIUrl":"10.1007/s40200-025-01659-9","url":null,"abstract":"<p><strong>Background: </strong>Diabesotension, an overlapping triad of diabetes, hypertension, and obesity, remains a diagnostic challenge due to its complex underlying molecular mechanisms. Individuals with diabesotension face twice the risk of microvascular and macrovascular complications compared to those with either condition alone. However, the complexity of diabesotension poses significant diagnostic challenges due to limited knowledge of this disease trifecta.</p><p><strong>Methods: </strong>The protein network was constructed, and the DPClusOST algorithm was applied to determine the protein clusters with a density ranging from 0.1 to 1.0 and those relevant to the pathophysiology of diabesotension. The significance score (SScore) was computed using the <i>p</i>-value from Fisher's exact test to evaluate each cluster, and the clusters containing proteins associated with diabesotension were classified using receiver operating characteristic (ROC) analysis. The significant density of the cluster, as indicated by the AUC, was determined and subsequently subjected to pathway enrichment analysis using ShinyGO.</p><p><strong>Results: </strong>At densities of 0.6 and 0.8, 14 proteins (STX3, VAMP2, STX4, SYT1, DNAJC5, HSD17B10, DLD, AIFM1, PDHA1, PDHB, DLAT, PDHX, OGDH, and STAT5A) from clusters 13 and 53 were significantly identified as potential diabesotension-related proteins. Key pathways associated with the tripartite interplay of the three pathologies were found to involve amino acid metabolism, glycolysis/gluconeogenesis, SNARE-mediated vesicle transport, insulin and salivary secretion, and the glucagon and HIF-1 signaling pathways, thus identifying novel candidates for diabesotension biomarkers and therapeutic targets.</p><p><strong>Conclusions: </strong>This study highlights the use of graph clustering to identify potential biomarkers for the comorbid triad, which could enhance personalized future treatment strategies.</p>","PeriodicalId":15635,"journal":{"name":"Journal of Diabetes and Metabolic Disorders","volume":"24 1","pages":"141"},"PeriodicalIF":1.8,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145358/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144258187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pattern of salt intake among Iranian hypertensive patients: insights from the 2021 STEPS study.","authors":"Amirhossein Ghaseminejad-Raeini, Keyvan Karimi, Nazila Rezaei, Yosra Azizpour, Moloud Payab, Mojtaba Lotfaliany, Mina Mirzad, Ali Golestani, Soroush Mozafari, Mahnaz Pejman Sani, Samaneh Akbarpour","doi":"10.1007/s40200-025-01649-x","DOIUrl":"10.1007/s40200-025-01649-x","url":null,"abstract":"<p><strong>Objectives: </strong>Excessive salt intake is a global health concern, particularly for individuals with hypertension (HTN). Limited research has examined the relationship between salt consumption and blood pressure control in Iranians. This study aimed to evaluate daily salt intake among hypertensive Iranians and its association with blood pressure management.</p><p><strong>Methods: </strong>Data were obtained from Iran's 2021 STEPS survey. Sample hypertensive patients were defined by self-report, antihypertensive medication use, or blood pressure (BP) measurements (SBP ≥ 140 mmHg or DBP ≥ 90 mmHg). Daily salt intake was estimated using the Tanaka method based on spot urine sodium levels and categorized based on calculated quartiles. BP was analyzed in both continuous and binary forms (controlled/uncontrolled). Logistic regression was applied to assess relationship between daily salt intake and uncontrolled hypertension as outcome.</p><p><strong>Results: </strong>The final analysis included 6,795 participants who were divided into four quartiles according to their daily salt intake. With a mean daily salt intake of 10.1 g [95% Confidence Interval (CI): 9.9-10.3], participants with undiagnosed hypertension consumed the most salt, (10.1 g [95% CI: 9.9-10.3], <i>p</i> = 0.002). With odds ratios (ORs) of 1.42 [95% CI: 1.16-1.74] (<i>p</i> = 0.001) and 1.32 [95% CI: 1.07-1.63] (<i>p</i> = 0.008), respectively, the third and fourth quartiles in the crude model showed noticeably higher odds of uncontrolled hypertension than the lowest quartile. Following the full adjustment model, the odds of uncontrolled hypertension were not significantly different in the fourth quartile compared to the first quartile (OR = 1.10 [95% CI: 0.87-1.39]; <i>p</i> = 0.404).</p><p><strong>Conclusions: </strong>Since increased salt intake raises SBP and DBP, particularly in those who are unaware of their condition, this study emphasizes the urgent need for public health interventions, such as nutritional counselling for low-salt diet, to reduce salt intake, especially in the Iranian population suffering from hypertension.</p>","PeriodicalId":15635,"journal":{"name":"Journal of Diabetes and Metabolic Disorders","volume":"24 1","pages":"142"},"PeriodicalIF":1.8,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144258189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of hearing function in patients with type 2 diabetes mellitus and investigation of its relationship with stages of diabetic retinopathy.","authors":"Medine Gündoğan, Fatih Gündoğan, Nurettin Bayram, Süleyman Demircan, Cemil Mutlu","doi":"10.1007/s40200-025-01660-2","DOIUrl":"10.1007/s40200-025-01660-2","url":null,"abstract":"<p><strong>Background: </strong>Diabetes mellitus is a serious, chronic metabolic disorder associated with microvascular and macrovascular complications that affect the cardiac, renal, ophthalmic, cerebral, and peripheral systems. Similar complications can also occur in the structures of the inner ear.</p><p><strong>Objectives: </strong>This study aimed to assess the hearing function of patients diagnosed with T2DM and to investigate the relationship between hearing function and the stage of diabetic retinopathy.</p><p><strong>Methods: </strong>This prospective, single-center, cross-sectional, and case-control study included 30 healthy controls (Group 1) and 90 patients with T2DM [30 without retinopathy (Group 2), 30 with non-proliferative diabetic retinopathy (Group 3), and 30 with proliferative diabetic retinopathy (Group 4)] aged 40 to 60 years. The best-corrected visual acuity and audiological tests, including pure tone audiometry (PTA) and high frequency audiometry (HFA), were performed.</p><p><strong>Results: </strong>A statistically significant difference was observed at all frequencies (for all, <i>p</i> < 0.001), except for 125 Hz (for 125 Hz, <i>p</i> = 0.075). The median PTA values were 13.75 (min-max: 5-31.2) in Group 1, 20 (min-max: 10-37.5) in Group 2, 16.87 (min-max: 8.7-42.5) in Group 3, and 24.37 (min-max: 12.5-56.25) in Group 4. A multiple comparisons analysis for PTA showed a statistically significant difference between Group 1 and Group 4 (<i>p</i> < 0.001). The mean HFA values were 32.23 ± 12.27 in Group 1, 50.23 ± 12.80 in Group 2, 53.47 ± 10.75 in Group 3, and 62.63 ± 12.52 in Group 4. The HFA values were significantly lower in Group 1 compared to the other groups (<i>p</i> < 0.001). In pairwise comparisons, <i>p</i> = 0.730 for Group 2 vs. Group 3, <i>p</i> = 0.001 for Group 2 vs. Group 4, and <i>p</i> = 0.021 for Group 3 vs. Group 4.</p><p><strong>Conclusions: </strong>It was observed that T2DM patients were at risk for developing hearing impairment, and sensorineural hearing impairment increased as the DR stage progressed, particularly in high frequencies. These results suggest a hearing evaluation in the screening of DR patients.</p>","PeriodicalId":15635,"journal":{"name":"Journal of Diabetes and Metabolic Disorders","volume":"24 1","pages":"143"},"PeriodicalIF":1.8,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145354/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144258188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The power of insulin education: enhancing pain and treatment perception in adult type 2 diabetes patients.","authors":"Mehmet Hayrullah Öztürk, Müjde Kerkez, Serap Parlar Kılıç","doi":"10.1007/s40200-025-01652-2","DOIUrl":"10.1007/s40200-025-01652-2","url":null,"abstract":"<p><strong>Objective: </strong>This study was conducted in order to evaluate the effect of insulin administration training provided to patients with Type 2 diabetes mellitus who use insulin on their safe medication usage, pain levels, and treatment perception.</p><p><strong>Methodology: </strong>A randomized controlled trial was designed. This study was conducted with a total of 84 patients (43 in the experimental group and 41 in the control group) diagnosed with Type 2 diabetes who were admitted to a public hospital in the Southeastern Anatolia Region of Turkey between October 2022 and March 2023. The patient information form, Insulin Administration Skill Observation Form, Visual Analog Scale, and Insulin Treatment Appraisal Scale were used. The experimental group was provided with individualized training and a booklet on the use of insulin.</p><p><strong>Results: </strong>Both posttest and retention test measurements were taken after the insulin administration training. According to these measurements, insulin administration skill and insulin treatment perceptions of the experimental group were significantly higher than those of the control group, while their pain intensity score was significantly lower (<i>p</i> < 0.001).</p><p><strong>Conclusion: </strong>The results of this study revealed that the insulin administration training increased safe medication application and insulin treatment perceptions of the patients in the experimental group and lowered their pain levels.</p><p><strong>Trial registration: </strong>ClinicalTrials.gov = NCT05915338.</p>","PeriodicalId":15635,"journal":{"name":"Journal of Diabetes and Metabolic Disorders","volume":"24 1","pages":"140"},"PeriodicalIF":1.8,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12141692/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperglycemia correlated with elevated circulating aminoacylase 1 in patients with pre-diabetes and type 2 diabetes.","authors":"Muataz Jalil Hussein Al-Maliki, Mostafa Allahyari, Ahmad Abbasi, Somayeh Sadani, Marie Saghaeian Jazi","doi":"10.1007/s40200-025-01646-0","DOIUrl":"10.1007/s40200-025-01646-0","url":null,"abstract":"<p><strong>Purpose: </strong>Type 2 diabetes is a common metabolic disorder characterized by hyperglycemia and insulin resistance. The metabolism of amino acids is also affected in diabetes, particularly through gluconeogenesis and increased catabolism of branched-chain amino acids. The aminoacylase 1 (ACY-1) enzyme plays a crucial role in the hydrolysis of N-acetylated amino acids, leading to the release of free amino acids. In this study, we aimed to investigate the circulating level of ACY-1 in individuals with type 2 diabetes mellitus (T2DM) and its correlation with glycemic factors.</p><p><strong>Methods: </strong>A total of 30 subjects with T2DM, 30 pre-diabetic individuals, and 30 control subjects were enrolled in the current study. Serum levels of ACY-1 and insulin were measured using ELISA method. Fasting Blood Sugar (FBS), 2-hour Postprandial (2hPP) glucose, HbA1c, urea, and creatinine levels were measured using an auto-analyzer. Insulin resistance was calculated using HOMA-IR formula.</p><p><strong>Results: </strong>We found elevated levels of serum ACY-1 in individuals with diabetes compared to those with pre-diabetes (<i>P</i> = 0.04) and the control group (<i>P</i> < 0.0001). Additionally, the pre-diabetes group exhibited significantly higher levels of ACY-1 than control group (<i>P</i> = 0.0093). Furthermore, a correlation was observed between ACY-1 and glycemic parameters, including insulin (<i>R</i>=-0.263, <i>P</i> = 0.014), FBS (<i>R</i> = 0.453, <i>P</i> = 0.00001), and HbA1c (<i>R</i> = 0.382, <i>P</i> = 0.016), which may have clinical significance.</p><p><strong>Conclusion: </strong>Patients with T2DM and individuals with pre-diabetes exhibit elevated serum levels of the aminoacylase-1 enzyme, which correlate with hyperglycemic factors such as FBS, HbA1c, and insulin. This finding underscores the significance of ACY-1 in the pathogenesis and progression of type 2 diabetes.</p>","PeriodicalId":15635,"journal":{"name":"Journal of Diabetes and Metabolic Disorders","volume":"24 1","pages":"139"},"PeriodicalIF":1.8,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12133664/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144234313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The relationship between depression, anxiety and health literacy with the severity of diabetes: the mediating role of fear of disease progression.","authors":"Fatemeh Fallah, Mohammad Kazem Atef Vahid, Mahdieh Salehi","doi":"10.1007/s40200-025-01650-4","DOIUrl":"10.1007/s40200-025-01650-4","url":null,"abstract":"<p><strong>Objective: </strong>Diabetes is a widespread metabolic and chronic disease worldwide. Similar to other chronic diseases, psychological factors may play a role in the course of this disease. In this study, an attempt was made to examine the relationship between depression, anxiety and health literacy with the severity of diabetes and the mediating role of fear of disease progression.</p><p><strong>Methods: </strong>A total of 225 patients with diabetes who visited the specialized and sub-specialized diabetes clinic in Amol city were selected through the they were selected using a convenient sampling method and a systematic approach based on inclusion criteria, and answered the 9-item Patient Health Questionnaire, Beck Anxiety Inventory, short form of Fear of Disease Progression and Health Literacy Inventory. To measure the severity of diabetes, HbA1c levels from the last blood test were measured. Structural equation modeling was used to test a hypothesized model. According to the fit indices, CMIN/df = 3.34, Goodness-of-fit index = 0.90, Normed Fit Index = 0.91, Comparative Fit Index = 0.90, and Root Mean Square Error of Approximation = 0.08 indicated an acceptable fit of the research model. The data were analyzed using SPSS version 26 and LISREL version 8/80 software.</p><p><strong>Results: </strong>The findings showed significantly positive relationships between anxiety (β = 0.29, <i>r</i> = 0.552), depression (β‌ = 0.13, <i>r</i> = 0.485), fear of disease progression (β = 0.14, <i>r</i> = 0.486) and the severity of diabetes. Also, a significantly negative relationship was observed between health literacy and severity of diabetes (β = -0.4, <i>r</i> = -0.564). In addition, it was shown that in diabetic patients, the fear of disease progression plays a mediating role in the relationship between depression (B = 0.03, T-value = 2.12), anxiety (B = 0.03, T-value = 2.42) and health literacy (B = -0.03, T-value = -2.69) with the disease severity.</p><p><strong>Conclusion: </strong>Therapeutic interventions to reduce the level of anxiety, depression and fear of disease progression and improve the level of health literacy of patients with diabetes can help in reducing the severity of diabetes. The generalizability of the results to other population requires confirmation by further research.</p>","PeriodicalId":15635,"journal":{"name":"Journal of Diabetes and Metabolic Disorders","volume":"24 1","pages":"137"},"PeriodicalIF":1.8,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12130382/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144225664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The novel role of <i>Periploca laevigata</i> Aiton extracts as antidiabetic and anti-inflammatory functions in pancreatic Β cells exposed to hyperglycaemia.","authors":"Ghada Trabelsi, Susana Mellado, Zahar Kalboussi, Leila Chekir-Ghedira, María Pascual","doi":"10.1007/s40200-025-01654-0","DOIUrl":"10.1007/s40200-025-01654-0","url":null,"abstract":"<p><p>Type 2 diabetes (T2D) is a major health issue characterized by glucose intolerance and insulin resistance, resulting in elevated blood glucose levels and insufficient insulin production by the pancreatic β-cells. This dysfunction triggers a chronic inflammatory immune response. Given the documented benefits of medicinal plants in managing diabetic complications and the therapeutic potential of <i>Periploca laevigata</i> Aiton (PL) extracts, this study evaluated their antidiabetic and anti-inflammatory properties using the pancreatic β-cell line INS-1E under hyperglycemic conditions. Root, fruit, and Leaf extracts of PL were prepared, phytochemically characterized, and assessed for anti-inflammatory effects via RT-qPCR in glucose-treated INS-1E cells. A non-cytotoxic concentration (0.125 mg/mL) was selected for all assays. Phytochemical screening revealed the presence of tannins and flavonoids, compounds associated with antidiabetic and anti-inflammatory activity. Notably, PL extracts upregulated insulin gene expression, with Leaf extracts exhibiting the most pronounced effects: they significantly enhanced GLUT-2 and PDX-1 gene expression in glucose-treated INS-1E cells. Additionally, PL extracts downregulated iNOS while upregulating IL-10 gene expression. A trend toward reduced NF-κB and MCP-1 gene expression was also observed. Collectively, these results suggest that PL extracts could improve pancreatic β-cell function and mitigate inflammation, highlighting their potential as adjunct therapy for T2D.</p>","PeriodicalId":15635,"journal":{"name":"Journal of Diabetes and Metabolic Disorders","volume":"24 1","pages":"136"},"PeriodicalIF":1.8,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12130407/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144225663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of aerobic exercise on cardiac histology and stereological parameters in a rat model of type 2 diabetes mellitus.","authors":"Alireza Rashidpour, Elaheh Piralaiy, Gholamreza Hamidian, Badrkhan Rashwan Ismael","doi":"10.1007/s40200-025-01641-5","DOIUrl":"10.1007/s40200-025-01641-5","url":null,"abstract":"<p><strong>Purpose: </strong>This study investigated the cardioprotective effects of an eight-week aerobic exercise program on cardiac histological and stereological parameters in rats with type 2 diabetes mellitus (T2DM), with a focus on structural remodeling and tissue composition.</p><p><strong>Methods: </strong>Thirty-two male Wistar rats were randomly assigned to four groups: diabetic exercise (Dia + Exe), healthy exercise (Heal + Exe), diabetic control (Dia + Con), and healthy control (Heal + Con) groups. T2DM was induced via a high-fat diet combined with a low-dose streptozotocin injection. The aerobic exercise protocol involved progressive treadmill training five days per week for eight weeks. Stereological analysis, which is based on systematic uniform random sampling, was conducted to assess cardiac dimensions, tissue composition, and myocyte morphology. Histological evaluation was performed via hematoxylin and eosin (H&E) staining.</p><p><strong>Results: </strong>Eight weeks of aerobic exercise significantly mitigated diabetes-induced cardiac atrophy, increasing the absolute heart weight by 48.9% (0.911 ± 0.048 g vs. 0.612 ± 0.057 g, p < 0.001) and normalizing the relative heart weight. Exercise led to a 41.4% increase in total cardiac volume, a 103% increase in cardiac muscle volume, and improvements in myocyte dimensions in diabetic rats. Additionally, exercise reduced pathological remodeling by decreasing connective tissue volume (7.1% reduction) and restoring vascular bed architecture (22.4% reduction), with significant exercise × diabetes interactions (p < 0.01).</p><p><strong>Conclusion: </strong>This study provides robust stereological evidence that aerobic exercise protects against diabetic cardiomyopathy by reversing cardiac atrophy, increasing muscle volume, reducing pathological fibrosis, and normalizing vascular architecture. These findings highlight aerobic exercise as a promising nonpharmacological intervention for preventing diabetes-induced cardiac structural deterioration.</p>","PeriodicalId":15635,"journal":{"name":"Journal of Diabetes and Metabolic Disorders","volume":"24 1","pages":"138"},"PeriodicalIF":1.8,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12130402/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144225662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between dietary micronutrient adequacy index and sleep quality, sleep duration and chronic fatigue in patients with type 2 diabetes.","authors":"Shervin Kazeminejad, Ensieh Nasli Esfahani, Leila Azadbakht","doi":"10.1007/s40200-025-01642-4","DOIUrl":"10.1007/s40200-025-01642-4","url":null,"abstract":"<p><strong>Purpose: </strong>A great number of individuals with type 2 diabetes mellitus (T2DM) suffer from sleep disturbances and chronic fatigue. Despite the potential role of diet in improving these problems, the relation between dietary micronutrients and these complications among individuals with T2DM has been less studied. This study aimed to investigate the associations between dietary micronutrient adequacy index (DMAI) and sleep quality, sleep duration, and chronic fatigue among patients with T2DM.</p><p><strong>Methods: </strong>In this cross-sectional study, 260 patients with T2DM aged 30-60y were enrolled. Sleep status and chronic fatigue were assessed using the Pittsburgh Sleep Quality Index and Chalder Fatigue Scale, respectively. Dietary intakes were collected through a validated food frequency questionnaire. DMAI was computed based on 14 micronutrients, with higher scores indicating greater adequacy. Binary logistic regression was employed to examine the relation between DMAI and study outcomes.</p><p><strong>Results: </strong>The mean age of participants was 50.42 ± 6.11 years. After adjusting all confounders, participants with the highest DMAI had significantly lower odds of poor sleep quality (OR = 0.32; 95% CI: 0.13, 0.79) and short sleep duration (OR = 0.16; 95% CI: 0.06, 0.44) compared to those with the lowest. However, DMAI was not significantly related to chronic fatigue in the fully adjusted model (OR _{T3 vs. T1} = 0.31; 95% CI: 0.09, 1.04).</p><p><strong>Conclusions: </strong>This study suggests that higher DMAI, indicating higher intakes of micronutrients, was associated with a reduced likelihood of poor sleep quality and short sleep duration, but not chronic fatigue among patients with T2DM. Prospective studies are recommended to replicate these findings.</p>","PeriodicalId":15635,"journal":{"name":"Journal of Diabetes and Metabolic Disorders","volume":"24 1","pages":"135"},"PeriodicalIF":1.8,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125449/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TMAO promotes metabolic dysfunction-associated fatty liver disease (MAFLD) development through long-non coding RNA- highly upregulated liver cancer (HULC).","authors":"Mohammad Moradzad, Mohammad Raman Moloudi, Alina Abdollahi, Shohreh Fakhari, Zakaria Vahabzadeh","doi":"10.1007/s40200-025-01605-9","DOIUrl":"10.1007/s40200-025-01605-9","url":null,"abstract":"<p><strong>Objectives: </strong>Trimethylamine N-oxide (TMAO) is related to the pathogenesis of Metabolic dysfunction-associated fatty liver disease (NAFLD). However, the molecular mechanism of how TMAO causes MAFLD development is still unclear. The present study attempted to investigate whether TMAO contributes to MAFLD development through HULC in a cellular model of MAFLD.</p><p><strong>Methods: </strong>HepG2 cells were cultured and induced in a fatty liver cell model. HULC knockdown was induced using the CRISPR/Cas13 system. Fatty liver cells were exposed to TMAO concentrations (75µM and 300µM) before and after HULC knockdown. RT-qPCR was used to evaluate the expression of the target genes. Apoptosis was assessed using Annexin V-FITC and PI staining. Statistical analyses included ANOVA and post-hoc tests.</p><p><strong>Results: </strong>TMAO upregulated the expression of HULC, followed by P38MAPK overexpression (<i>P</i> value < 0.05). Upon HULC knockdown, TMAO could not change P3MAPK expression and its downstream targets, including TNFα, IL-6, and PNPPLA3 in fatty liver cells. Additionally, TMAO significantly induced apoptosis in the fatty acid cellular model (<i>P</i> value < 0.05).</p><p><strong>Conclusion: </strong>In conclusion, the results of this study provide evidence of the TMAO/HULC/P38MAPK axis involvement in the pathogenesis of MAFLD by increasing the expression of genes involved in inflammation and fibrosis. Our data suggests that TMAO reduction could be a therapeutic target in MAFLD through gut microbiome modulation.</p>","PeriodicalId":15635,"journal":{"name":"Journal of Diabetes and Metabolic Disorders","volume":"24 1","pages":"131"},"PeriodicalIF":1.8,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125414/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}