Journal of Dental Sciences最新文献

{"title":"The cell-fate decision of dental follicle stem cells (DFSCs) with porcine corneal extracellular matrix (ECM)","authors":"Kuan-Ming Lin ,&nbsp;Yong-Ren Chen ,&nbsp;Ming-Hua Ho ,&nbsp;Chung-Hsing Li","doi":"10.1016/j.jds.2025.02.019","DOIUrl":"10.1016/j.jds.2025.02.019","url":null,"abstract":"<div><h3>Background/purpose</h3><div>Extracellular matrix (ECM) may be useful as a natural scaffold for tissue engineering. Since dental follicle stem cells (DFSCs) share a similar embryonic origin with corneal keratocytes and endothelial cells and possess the ability to proliferate and differentiate, in this study, we investigate the potential of DFSCs to differentiate into corneal cells under the supercritical carbon dioxide (SC–CO₂) decellularized porcine corneal ECM culture stimulation.</div></div><div><h3>Materials and methods</h3><div>DFSCs were seeded onto the SC-CO₂ decellularized porcine corneal ECM and the surrounding culture dish for 21 days. Cell morphology, reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and immunofluorescence staining were used to evaluate the potential of DFSCs to differentiate into corneal epithelial, keratocyte, or endothelial cells. The culture medium was collected every 3 days for growth factor analysis.</div></div><div><h3>Results</h3><div>The DFSCs cultured on the ECM exhibit notable cell-ECM adhesion and the potential to primarily differentiate into keratocytes, with a partial capacity for differentiation into corneal endothelial cells, while the cells outside the ECM are weakly affected. Growth factor array analysis revealed changes in growth factor expression in different culture conditions, DFSCs, ECM, and DFSCs/ECM co-culture at various time points, indicating a dynamic shift in the microenvironment.</div></div><div><h3>Conclusion</h3><div>The present study demonstrated that DFSCs cultured on the porcine corneal ECM can differentiate mainly into keratocytes-like and partially into corneal endothelial-like cells, exhibiting strong cell-ECM adhesion and regulation of growth factors. Provide insights into the therapeutic application potential of dental-derived stem cells and SC-CO₂ decellularized tissue for corneal diseases.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 3","pages":"Pages 1605-1614"},"PeriodicalIF":3.4,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144366771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The activities of oral health care for high school students in Taipei City during the Japanese colonial period","authors":"Feng-Chou Cheng ,&nbsp;Ling-Hsia Wang ,&nbsp;Chun-Pin Chiang","doi":"10.1016/j.jds.2025.02.021","DOIUrl":"10.1016/j.jds.2025.02.021","url":null,"abstract":"","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 3","pages":"Pages 1940-1943"},"PeriodicalIF":3.4,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144364838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined tomography and conventional impression with notch system to fabricate prosthesis with palatal obturator in patients with severe limited mouth opening","authors":"Chloé Mense ,&nbsp;Frédéric Silvestri ,&nbsp;Charlotte Mansuy ,&nbsp;Cassandre Bezier ,&nbsp;Romain Lan","doi":"10.1016/j.jds.2025.02.020","DOIUrl":"10.1016/j.jds.2025.02.020","url":null,"abstract":"<div><div>In oral cancer therapies, surgery or radiotherapy frequently result to maxillary defects and limited mouth opening (LMO). For prosthetic rehabilitation, these sequelae present significant challenges to achieving accurate impressions. Intraoral scanners (IOS) are unsuitable due to the size of the scanner tips, while conventional impression techniques risk tearing during tray removal. Additionally, cone-beam computed tomography (CBCT) alone, without superimposed IOS data, lacks the precision required due to insufficient tissue precision and artefacts. To address these limitations, a novel hybrid technique combining CBCT and conventional impressions was presented in 3 patients. A 3D-printed model was created from CBCT data, which serves as base for fabricating a palatal plate with notches. This plate is used to capture a conventional impression of the dentition, and the two components are aligned extraorally using notches. This approach ensures capture of hard and soft tissues while reducing patient discomfort with severe LMO during the impression process.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 3","pages":"Pages 1908-1911"},"PeriodicalIF":3.4,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144366919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corylin ameliorates inflammaging and pyroptosis in diabetic periodontitis: A preliminary in vitro study","authors":"Taichen Lin ,&nbsp;Min Yee Ng ,&nbsp;Chun-Te Ho ,&nbsp;Yi-Wen Liao ,&nbsp;Cheng-Chia Yu ,&nbsp;Chun-Jung Chen","doi":"10.1016/j.jds.2025.02.014","DOIUrl":"10.1016/j.jds.2025.02.014","url":null,"abstract":"<div><h3>Background/purpose</h3><div>Diabetic periodontitis (DP) is a severe oral disease characterized by hyperinflammation and impaired wound healing, with inflammaging and pyroptosis playing key roles in its pathogenesis. Corylin, an isoflavone compound, has shown promising anti-inflammatory and anti-pyroptotic properties, but its specific effects on DP remain largely unexplored. This study aimed to evaluate the effects of Corylin on inflammaging and pyroptosis in an in vitro model of DP, potentially offering novel insights into therapeutic strategies for this challenging condition.</div></div><div><h3>Materials and methods</h3><div>This in vitro study evaluated the effects of Corylin on inflammaging and pyroptosis in human gingival fibroblasts (HGFs) exposed to advanced glycation end products (AGEs) to mimic the diabetic environment. We then examined the reactive oxygen species (ROS) generation and wound healing ability in the cells. To assess the inflammaging, we probed into cell senescence activity and senescence marker p16 as well as its senescence associated secretory phenotype (SASP) such as interleukins (IL)-6 and IL-8. Next, we measured the levels of pyroptosis markers including nucleotide-binding domain, leucine-rich–containing family, pyrin domain–containing-3 (NLRP3), apoptosis-associated speck-like protein containing a CARD (ASC), caspase-1 in cells with and without Corylin.</div></div><div><h3>Results</h3><div>Corylin reduced ROS production and enhanced wound healing in AGEs-treated HGFs in a dose-dependent manner. Furthermore, Corylin attenuated the heightened inflammaging markers, which included cellular senescence and the secretion of SASP, IL-6 and IL-8. Additionally, Corylin downregulated the expression of pyroptosis-related components, including NLRP3, ASC, and caspase-1, in AGEs-treated HGFs.</div></div><div><h3>Conclusion</h3><div>These findings suggest that Corylin may have therapeutic potential in DP by mitigating AGE-induced inflammaging and pyroptosis. Corylin's ability to promote wound healing and inhibit both cellular senescence and pyroptosis highlights its potential as a novel therapeutic agent for DP.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 3","pages":"Pages 1615-1621"},"PeriodicalIF":3.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144366772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A study of critical thinking skills among Thai dental students: From disposition to skills assessment","authors":"Kittichai Sireerat ,&nbsp;Naoko Seki ,&nbsp;Richard Foxton ,&nbsp;Shin-Ru Liao ,&nbsp;Masako Akiyama ,&nbsp;Supaporn Sangouam ,&nbsp;Ikuko Morio","doi":"10.1016/j.jds.2025.02.005","DOIUrl":"10.1016/j.jds.2025.02.005","url":null,"abstract":"<div><h3>Background/purpose</h3><div>Critical thinking skills might be influenced by culture. There has been no published research to date, on the critical thinking skills of Asian undergraduate students and so this study's purpose was to explore the critical thinking skills of Thai undergraduate dental students to elucidate their level and whether there might be need for training to improve their critical thinking.</div></div><div><h3>Materials and methods</h3><div>Participants were second- (n = 63) and fifth-year (n = 51) undergraduate dental students in the 2023 academic year at Naresuan University, Thailand. A questionnaire was distributed, which included questions on demographic information and a Thai version of the California Critical Thinking Skills Test (CCTST).</div></div><div><h3>Results</h3><div>The response rate was 100 % and the overall mean CCTST score of the students was 15.34 (SD 3.6). There were no statistically significant differences between the two years (<em>P</em> &gt; 0.05), and “Induction” was not manifested. There was no significant correlation between GPA and all the domains of the CCTST scores; however, there was a significant correlation between students’ frequency of critical thinking use and overall CCTST score (<em>P</em> = 0.047, ρ = 0.187) and what students thought about the importance of critical thinking and their frequency of critical thinking use (<em>P</em> &lt; 0.01, ρ = 0.337).</div></div><div><h3>Conclusion</h3><div>The critical thinking skills scores of all the undergraduate Thai dental students were found to be moderate indicating a need for interventions aimed at improving all the domains of critical thinking skills in particular the domain of “Induction” in the undergraduate curriculum.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 3","pages":"Pages 1622-1628"},"PeriodicalIF":3.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144366814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-cell genomics reveals transcriptional heterogeneity and cellular crosstalk in salivary gland fibrosis","authors":"Dan Liang ,&nbsp;Wei Xu ,&nbsp;Yifan Wu ,&nbsp;Qicheng Ye ,&nbsp;Wanlin Xu ,&nbsp;Hao Lu","doi":"10.1016/j.jds.2025.02.015","DOIUrl":"10.1016/j.jds.2025.02.015","url":null,"abstract":"<div><h3>Background/purpose</h3><div>Salivary gland fibrosis (SGF) is a prevalent condition associated with various pathological states. However, a detailed understanding of the single-cell transcriptional profiles and cell–cell communication networks in this context remains elusive. Therefore, we aimed to unveil the gene expression profiles and intercellular communication patterns in SGF.</div></div><div><h3>Materials and methods</h3><div>We established fibrosis models of the submandibular gland (SMG) and generated a detailed single-cell transcriptomic atlas to investigate cellular heterogeneity and communication patterns. Utilizing CellChat, we dissected the intercellular dialogues within these fibrotic environments.</div></div><div><h3>Results</h3><div>The analysis distinguished fifteen distinct cellular clusters, highlighting significant heterogeneity among fibroblasts in fibrotic SMGs compared to their healthy counterparts. This heterogeneity was linked to TGF-β signaling, collagen fibril assembly, and extracellular matrix organization. CellChat analyses uncovered enhanced cell–cell interactions, notably between fibroblasts and endothelial cells (ECs) via the Gas6-Axl signaling axis. Additionally, our data indicated the activation of cell adhesion molecules and Rap1 pathways in ECs within the fibrotic SMG microenvironment.</div></div><div><h3>Conclusion</h3><div>This study offers an in-depth single-cell resolution perspective on the cellular and molecular changes in SMG fibrosis, identifying new potential therapeutic targets for this condition.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 3","pages":"Pages 1406-1414"},"PeriodicalIF":3.4,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144366536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of risk management for orthodontists’ practice in Taiwan","authors":"Sheng-Huai Wang ,&nbsp;Johnson Hsin-Chung Cheng ,&nbsp;Kuan Chung Huang ,&nbsp;Daniel De-Shing Chen","doi":"10.1016/j.jds.2025.02.010","DOIUrl":"10.1016/j.jds.2025.02.010","url":null,"abstract":"<div><h3>Background/Purpose</h3><div>Risk management in the health-care industry is a systematic approach for mitigating potential risks and enhancing patient safety. This study examined orthodontists’ perceptions of and attitudes toward risk management in Taiwan. The results provide clinical references informing policies for health-care institutions in Taiwan.</div></div><div><h3>Materials and methods</h3><div>During the 2021 Taiwan Association of Orthodontics (TAO) annual meeting, a structured questionnaire was distributed to 143 randomly selected orthodontists. This questionnaire comprised 23 questions on demographics (5 questions), knowledge of risk management (5 questions), execution of and attitude toward risk management (8 questions), and adverse event experiences (7 questions). After the frequency distribution of each question was examined, demographics and participants’ preferences were analyzed through chi-square and multiple regression tests.</div></div><div><h3>Results</h3><div>Goodness-of-fit testing revealed no significant differences in sex or age between the participants and TAO members. Most of the orthodontists were employees (68.31%). Additionally, most of the orthodontists (90.14%) believed that a favorable doctor–patient relationship and high-quality orthodontic treatment are essential for mitigating the risks associated with orthodontic care. Moreover, 30.28% of the orthodontists reported AEs, and 38.73% reported foreign body ingestion by patients. No significant correlation was observed between demographics and risk management.</div></div><div><h3>Conclusion</h3><div>The majority of orthodontists in Taiwan have a positive understanding of and attitude toward risk management. Factors such as sex, age, and clinical experience have a minimal influence on the implementation of risk management practices by Taiwanese orthodontists. These findings provide valuable clinical references for governmental organizations aiming to formulate policies related to orthodontic practice and risk management.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 2","pages":"Pages 1213-1220"},"PeriodicalIF":3.4,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143706013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the effects for root resorption in orthodontic tooth movement with micro-osteoperforations in mice","authors":"Tulonga Ndemuweda ,&nbsp;Hideki Kitaura ,&nbsp;Fumitoshi Ohori ,&nbsp;Aseel Marahleh ,&nbsp;Jinghan Ma ,&nbsp;Ziqiu Fan ,&nbsp;Angyi Lin ,&nbsp;Kohei Narita ,&nbsp;Arata Itou ,&nbsp;Itaru Mizoguchi","doi":"10.1016/j.jds.2025.02.017","DOIUrl":"10.1016/j.jds.2025.02.017","url":null,"abstract":"<div><h3>Background/purpose</h3><div>Orthodontic treatment is one of the most demanding procedures available for both patients and clinicians. The challenges stem from the extended duration to achieve desired results, often necessitating surgical interventions, such as micro-osteoperforations (MOPs). This study aimed to investigate the biological effects and extent of changes resulting from these interventions. Specifically, we evaluated the degree of root resorption during orthodontic tooth movement accelerated by MOPs.</div></div><div><h3>Materials and methods</h3><div>We assessed the tooth movement rates and root resorption in eight-to ten-week-old male mice. A nickel-titanium (Ni–Ti) closed-coil spring was applied between the maxillary left first molar and maxillary incisors. In the MOPs group, micro-perforations were made on the mesial and palatal surfaces of the left maxillary first molar. Odontoclast formation and root resorption were assessed using histological analysis and scanning electron microscopy.</div></div><div><h3>Results</h3><div>Tooth movement was greater in the MOPs group. Odontoclast formation was remarkably higher in this group than in the orthodontic tooth movement (OTM) group. Additionally, more extensive root resorption was observed on the mesial surface of the distobuccal root of the left maxillary first molar.</div></div><div><h3>Conclusion</h3><div>Root resorption significantly increased in mice with MOPs. These findings highlight the need to carefully consider the risk of root resorption in patients undergoing MOPs during orthodontic treatment.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 3","pages":"Pages 1415-1421"},"PeriodicalIF":3.4,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The dental clinical internship for the medical students during the Japanese colonial period in Taiwan","authors":"Feng-Chou Cheng ,&nbsp;Ling-Hsia Wang ,&nbsp;Chun-Pin Chiang","doi":"10.1016/j.jds.2025.02.013","DOIUrl":"10.1016/j.jds.2025.02.013","url":null,"abstract":"","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 2","pages":"Pages 1276-1279"},"PeriodicalIF":3.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term stability of orthodontic implant site development: A 13-year case report","authors":"Cheng-Shan Li,&nbsp;Yuan-Yang Hsu,&nbsp;Ting-Hsin Huang,&nbsp;Shing-Zeng Dung","doi":"10.1016/j.jds.2025.02.011","DOIUrl":"10.1016/j.jds.2025.02.011","url":null,"abstract":"","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 2","pages":"Pages 1295-1297"},"PeriodicalIF":3.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}