Journal of Comparative Neurology最新文献_第7页

Proteomic Signatures of Regeneration: Uncovering Extracellular Matrix-Associated Cues in Goldfish Spinal Cord Repair. 再生的蛋白质组学特征：揭示金鱼脊髓修复中的细胞外基质相关线索。

IF 2.3 4区医学

Journal of Comparative Neurology Pub Date : 2026-05-01 DOI: 10.1002/cne.70170

Cátia P Dombaxe, Anthony R D Amato, Margaret Veerkamp, Allison Kwon, Osaiyekemwen Ogbemudia, Aminat M Ibrahim, Yadong Wang

{"title":"Proteomic Signatures of Regeneration: Uncovering Extracellular Matrix-Associated Cues in Goldfish Spinal Cord Repair.","authors":"Cátia P Dombaxe, Anthony R D Amato, Margaret Veerkamp, Allison Kwon, Osaiyekemwen Ogbemudia, Aminat M Ibrahim, Yadong Wang","doi":"10.1002/cne.70170","DOIUrl":"10.1002/cne.70170","url":null,"abstract":"Spinal cord injury (SCI) in mammals leads to inflammation, glial scarring, and extracellular matrix (ECM) remodeling that hinder regeneration. In contrast, certain teleosts, such as zebrafish and goldfish (Carassius auratus), exhibit remarkable spinal cord regenerative capabilities and substantial functional recovery following injury. To uncover the molecular basis of this regenerative ability, we performed a proteomic analysis of goldfish spinal cord ECM-enriched post-injury and compared it to literature on mammalian models. We identified a distinct regenerative signature in goldfish, marked by the downregulation of vimentin A2, histone H1-like isoform X1, connexin 43, and biglycan-like proteins, factors typically associated with scarring and inflammation in mammals. Notably, nucleosome assembly protein 1-like 4 (NAP1L4), a chromatin remodeling protein, was uniquely upregulated in goldfish, suggesting a more permissive epigenetic environment for repair. These findings provide new insights into the molecular determinants of successful spinal cord regeneration and may inform strategies for developing regenerative therapies in humans. Teaser: Goldfish spinal cords reveal a unique regenerative proteomic signature absent in non-regenerative mammals after injury.","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"534 5","pages":"e70170"},"PeriodicalIF":2.3,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13375165/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147972809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Dendritic Specializations in Two Classes of Kenyon Cells in the Mushroom Body of the Adult Honeybee, Apis mellifera. 成年蜜蜂蘑菇体中两类凯尼恩细胞树突特化的分析。

IF 2.3 4区医学

Journal of Comparative Neurology Pub Date : 2026-05-01 DOI: 10.1002/cne.70169

Andrea Rafaela Nicolaidou, Basil El Jundi, Wolfgang Rössler, Claudia Groh

{"title":"Analysis of Dendritic Specializations in Two Classes of Kenyon Cells in the Mushroom Body of the Adult Honeybee, Apis mellifera.","authors":"Andrea Rafaela Nicolaidou, Basil El Jundi, Wolfgang Rössler, Claudia Groh","doi":"10.1002/cne.70169","DOIUrl":"10.1002/cne.70169","url":null,"abstract":"The mushroom bodies (MBs) in the insect brain serve sensory integration and memory formation. In the honeybee, they house two classes of intrinsic neurons: class I (spiny) and II (clawed) Kenyon cells (KCs). Both classes form postsynaptic elements in synaptic complexes (microglomeruli) comprising large axonal boutons from olfactory and visual projection neurons. To adapt their neuronal information processing systems, MB microglomeruli undergo age-, memory-, and environment-related structural plasticity. To analyze KC dendritic specializations and their connections with presynaptic boutons, we combined tracer injections in small groups of KCs from different age cohorts (freshly emerged bees to foragers) with presynaptic anti-synapsin immunolabeling. Using high-resolution confocal 3D reconstructions, we analyzed shape and contacts of class I and II KC dendrites in the olfactory (lip) and visual (collar) input sites of the MB calyx. In both KC classes, dendrites are always restricted to either the lip or the collar. We classified two types of class II KCs regarding the spatial distribution of dendritic branches: large-clustered and small-distributed. Individual claws of class II KCs largely vary regarding surface areas covered on individual axonal boutons (∼5%-70%). In class I KCs, we found four distinct morphological spine categories: stubby, thin, mushroom-shaped, and branched. Interestingly, the overall frequency of putative spine-bouton contacts in class I KCs remains largely constant throughout age cohorts. We discuss the results in the light of structural dynamics in MB microglomerular circuits and their role in multisensory information processing.","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"534 5","pages":"e70169"},"PeriodicalIF":2.3,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13183002/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147972836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Na_V1.7 mRNA and Protein Expression in Resident Neurons of the Human Spinal Dorsal Horn. 人脊髓背角驻留神经元中NaV1.7 mRNA和蛋白的表达。

IF 2.3 4区医学

Journal of Comparative Neurology Pub Date : 2026-05-01 DOI: 10.1002/cne.70168

Stephanie Shiers, Nwasinachi A Ezeji, Carla Lima-Truax, Jeffrey L Krajewski, Geoffrey Funk, Anna Cervantes, Peter Horton, Gregory Dussor, Stephanie Hennen, Theodore J Price

{"title":"NaV1.7 mRNA and Protein Expression in Resident Neurons of the Human Spinal Dorsal Horn.","authors":"Stephanie Shiers, Nwasinachi A Ezeji, Carla Lima-Truax, Jeffrey L Krajewski, Geoffrey Funk, Anna Cervantes, Peter Horton, Gregory Dussor, Stephanie Hennen, Theodore J Price","doi":"10.1002/cne.70168","DOIUrl":"10.1002/cne.70168","url":null,"abstract":"The voltage-gated sodium channel NaV1.7 is a pain target supported by human genetics, and many compounds have been developed to inhibit NaV1.7 but have disappointed in clinical trials due to cardiovascular effects. Because some recent reports suggest that pharmacological inhibition of NaV1.7 in the rodent spinal dorsal horn can achieve pain relief, we sought to better understand NaV1.7 expression in the human spinal cord. We report that NaV1.7 mRNA is expressed in putative projection neurons (NK1R+, GPR83+) in the human spinal dorsal horn, predominantly in lamina I and II, as well as in deep dorsal horn neurons. NaV1.7 mRNA was also detected in preganglionic parasympathetic and sympathetic neurons, motor neurons in the ventral horn, and ependymal cells lining the central canal. NaV1.7 protein was predominantly found in the central axons of sensory neurons terminating in lamina I-II and colocalized, in part, with presynaptic markers like Bassoon and CGRP. Postsynaptically, NaV1.7 protein was detectable in the soma of motor neurons but was more elusive in dorsal horn populations due to the abundance of presynaptic signal. However, NaV1.7 protein was detected in the axon initial segment of some resident dorsal horn neurons and in axons entering the anterior commissure. Given that projection neurons are critical for conveying nociceptive information from the dorsal horn to the brain, these data support that dorsal horn NaV1.7 expression may play an unappreciated role in pain phenotypes observed in humans with genetic SCN9A mutations.","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"534 5","pages":"e70168"},"PeriodicalIF":2.3,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13178720/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147948423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Topographical Relationship in the Mesodiencephalic Projection to the Inferior Olive Linked With Cerebellar Hemispheric Lobules in Mice. 小鼠小脑半球小叶连接下橄榄的中间脑投射的地形关系。

IF 2.3 4区医学

Journal of Comparative Neurology Pub Date : 2026-05-01 DOI: 10.1002/cne.70167

Ji Qing, Izumi Sugihara

{"title":"Topographical Relationship in the Mesodiencephalic Projection to the Inferior Olive Linked With Cerebellar Hemispheric Lobules in Mice.","authors":"Ji Qing, Izumi Sugihara","doi":"10.1002/cne.70167","DOIUrl":"10.1002/cne.70167","url":null,"abstract":"The mesodiencephalic junction (MDJ) is located in a caudorostrally elongated, column-shaped region encompassing the medial accessory oculomotor nucleus and perirubral area. It relays cerebral projections to the rostral subnuclei of the inferior olive (IO), which, in turn, send climbing fibers to the cerebellar hemisphere and receive nucleo-olivary projections from the cerebellar nuclei (CN), forming a parallel, loop-shaped, modular circuitry. However, the topographical organization of the MDJ-IO projection, which may contribute to functional localization within the cerebellar hemisphere, has not been fully elucidated. Here, we analyzed the distribution of labeled neurons in the MDJ in relation to those in the CN by injecting a Lumafluor retrograde tracer into various sites within the rostral IO subnuclei of mice. Injections mainly targeting the lateral or medial parts of the rostral IO subnuclei labeled neurons predominantly in the ventral or dorsal CN, respectively (referred to as v- and d-CN-predominant injection cases). Correspondingly, v-CN-predominant injection cases labeled neurons in the rostromedial, centroventral, and caudolateral regions of the MDJ, collectively termed the \"sheathing subarea,\" whereas d-CN-predominant injection cases labeled neurons primarily in the central \"shaft subarea\" of the MDJ. Considering the previously reported lobule-related topography among the IO, cerebellar cortex, and CN, the sheathing and shaft subareas of the MDJ are likely to project preferentially to crus I and to other neighboring cerebellar hemispheric lobules, respectively. We speculate that the sheathing subarea of the MDJ is more strongly involved in non-somatosensorimotor cognitive functions than the shaft subarea.","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"534 5","pages":"e70167"},"PeriodicalIF":2.3,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13373634/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148015857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Sex- and Male-Morph-Specific Variation in Brain Mass and Cell Number Scaling in Solitary Centris pallida (Hymenoptera: Apidae) Bees 孤斑中蜂（膜翅目：蜂科）脑质量和细胞数目的性别和雄性形态特异性变异。

IF 2.1 4区医学

Journal of Comparative Neurology Pub Date : 2026-04-17 DOI: 10.1002/cne.70163

Meghan Barrett, R. Keating Godfrey

{"title":"Sex- and Male-Morph-Specific Variation in Brain Mass and Cell Number Scaling in Solitary Centris pallida (Hymenoptera: Apidae) Bees","authors":"Meghan Barrett, R. Keating Godfrey","doi":"10.1002/cne.70163","DOIUrl":"10.1002/cne.70163","url":null,"abstract":"Intraspecific variation in behavior is associated with variable brain resource allocation patterns: There is frequently increased tissue investment in discrete regions that support fitness-relevant cognitive abilities. However, the relationships between tissue volume and actual cell numbers have rarely been explored for insects due to methodological hurdles recently addressed via the application of isotropic fractionation. In solitary desert Centris pallida (Hymenoptera: Apidae) bees, there are two major levels of intraspecific variation: sex (males vs. females) and male morph (as a result of alternative reproductive tactics, large morph and small morph males rely on scent or sight, respectively, for mate location). Using isotropic fractionation, we separately analyzed optic lobe (OL) and central brain (CB) cell numbers of males and females to determine the impacts of sex and morph on brain cell allometry. Female bees’ brains were bigger and had higher cell numbers and cell densities than males of the same size. In both sexes, total brain cell number increased with brain size, driven by increases in OL cell numbers. Between male morphs, we found that OL masses were relatively larger in small-morph males, consistent with the relationship between body size and OL volumes reported in prior studies. However, small-morph C. pallida males had fewer total cells (as represented by cell nuclei) and reduced cell density, in their OLs. Together, these data suggest that there is intraspecific and brain-region-specific variation in brain cell numbers and that variation in brain tissue volume may not match other levels of neural organization like brain cell numbers/densities.","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"534 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13088941/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147698968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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IF 2.1 4区医学

Journal of Comparative Neurology Pub Date : 2026-04-17

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IF 2.1 4区医学

Journal of Comparative Neurology Pub Date : 2026-04-15

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IF 2.1 4区医学

Journal of Comparative Neurology Pub Date : 2026-04-15

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Additional Cover: Cover Image, Volume 534, Issue 4 附加封面：封面图像，第534卷，第4期

IF 2.1 4区医学

Journal of Comparative Neurology Pub Date : 2026-04-15 DOI: 10.1002/cne.70161

Lucía Inés Torrijos-Saiz, Marco Ghibaudi, Malaz Sharief, Lovisa Ljungqvist Brinson, Arturo Alvarez-Buylla, José Manuel García-Verdugo, Vicente Herranz-Pérez, Shawn Sorrells

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Featured Cover: Cover Image, Volume 534, Issue 4 特色封面：封面图像，第534卷，第4期

IF 2.1 4区医学

Journal of Comparative Neurology Pub Date : 2026-04-15 DOI: 10.1002/cne.70159

Defne Albayrak, Sinem Gencturk, Kevin B. Haselhuhn, Cem Sevinc, Onur Güntürkün, Mehdi Behroozi, Noemi Rook, Gunes Unal

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