Journal of Chromatography & Separation Techniques最新文献_第8页

Simultaneous Determination of Six Insecticides in Okra (Abelmoschus esculentus L. Moench) by UHPLC-MS/MS UHPLC-MS/MS同时测定秋葵中6种杀虫剂

Journal of Chromatography & Separation Techniques Pub Date : 2017-06-27 DOI: 10.4172/2157-7064.1000370

H. Hussan, Xingang Liu, F. Dong, Jun Xu, Yongquan Zheng

引用次数: 1

High Performance Liquid Chromatographic Determination of Naproxen in Prepared Pharmaceutical Dosage Form and Human Plasma and its Application to Pharmacokinetic Study 高效液相色谱法测定制剂剂型和人血浆中萘普生的含量及其药动学研究

Journal of Chromatography & Separation Techniques Pub Date : 2017-06-14 DOI: 10.4172/2157-7064.1000369

Saiqa Muneer, I. Muhammad, M. Abrar

{"title":"High Performance Liquid Chromatographic Determination of Naproxen in Prepared Pharmaceutical Dosage Form and Human Plasma and its Application to Pharmacokinetic Study","authors":"Saiqa Muneer, I. Muhammad, M. Abrar","doi":"10.4172/2157-7064.1000369","DOIUrl":"https://doi.org/10.4172/2157-7064.1000369","url":null,"abstract":"A simple, fast, economical and precise reverse phase liquid chromatographic method was developed, optimized and validated for quantification of naproxen sodium according to the standard guidelines. The separation of the analyte and internal standard was achieved over C-18 column using acetonitrile: water: glacial acetic acid as mobile phase in a ratio of 50:49:1 (v/v) in isocratic mode at a flow rate of 1.2 mL/min at a wavelength of 254 nm at ambient temperature. The retention time was found to be less than 10 minutes. The limit of detection (LOD) was 10 ng/mL and limit of quantification (LOQ) was 15 ng/mL. Naproxen sodium was extracted from biological samples by using acetonitrile as extraction solvent. The linearity was found to be 0.5 to 80 ppm. All the parameters were validated for accuracy, precision, linearity, sensitivity, reproductivity and stability. The method was successfully applied for the quantification of the naproxen sodium in animal and human plasma. Therefore, the method was found to be accurate, reproducible, sensitive, cost effective, less time consuming and can be successfully applied on routine analysis of naproxen sodium in pharmaceutical formulations and pharmacokinetic studies in human and animal models.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"20 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2017-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75216870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

SPE: A Time for Lowering Cost SPE:降低成本的时代

Journal of Chromatography & Separation Techniques Pub Date : 2017-06-05 DOI: 10.4172/2157-7064.1000367

S. Alrefaee, M. Hattab, S. Almosaad

引用次数: 0

HPLC-MS/MS Based Time Course Analysis of Morphine and Morphine-6-Glucoronide in ICU Patients 基于HPLC-MS/MS的ICU患者吗啡及吗啡-6-硫代糖苷的病程分析

Journal of Chromatography & Separation Techniques Pub Date : 2017-06-01 DOI: 10.4172/2157-7064.1000368

E. Andreas, J. Peter, M. Lerch, J. Schüttler, C. Jeleazcov

引用次数: 2

Loess Soil Nanoparticles as A Novel Adsorbent for Adsorption of Green Malachite Dye 黄土纳米颗粒吸附绿色孔雀石染料的研究

Journal of Chromatography & Separation Techniques Pub Date : 2017-05-24 DOI: 10.4172/2157-7064.1000366

Heydartaemeh Mr, S. Aslani, F. DoulatiArdejani

引用次数: 5

Forced Degradation Study of Monoclonal Antibody Using Two-Dimensional Liquid Chromatography 单克隆抗体的二维液相色谱强制降解研究

Journal of Chromatography & Separation Techniques Pub Date : 2017-05-17 DOI: 10.4172/2157-7064.1000365

Y. An, S. Verma, Y. Chen, S. Yu, Y. Zhang, S. Kelner, S. Mengisen, D. Richardson, Z. Chen

{"title":"Forced Degradation Study of Monoclonal Antibody Using Two-Dimensional Liquid Chromatography","authors":"Y. An, S. Verma, Y. Chen, S. Yu, Y. Zhang, S. Kelner, S. Mengisen, D. Richardson, Z. Chen","doi":"10.4172/2157-7064.1000365","DOIUrl":"https://doi.org/10.4172/2157-7064.1000365","url":null,"abstract":"Monoclonal antibody (mAb) products are extraordinarily heterogeneous due to the presence of a variety of enzymatic and chemical modifications, such as deamidation, isomerization, oxidation, glycosylation, glycation, terminal cyclization, aggregation, and fragmentation. Forced degradation study is a common practice to assess the potential modifications and degradation pathways of mAbs upon extreme conditions, including light exposure and extreme local pH. The degraded samples are subject to characterization using a wide array of analyses, including ion exchange chromatography (IEC) for charge variants and size exclusion chromatography (SEC) for size variants. In this proof-of-concept study, a two-dimensional liquid chromatography (2D-LC) approach was successfully applied to mAb forced degradation samples for evaluation of size distribution in the IEC charge profile. The charge variant peaks in first-dimension (1D) IEC were fractioned by a heart-cutting schedule, and the integrated peak parking feature accommodated later-eluting 1D cuts when the previous ones were still under second-dimension (2D) analysis. Thus, for a singly IEC injection, all IEC fractions were acquired by the 2D for SEC analysis. This study demonstrated that 2D SEC was compatible with 1D IEC for 2D-LC analysis. The method sensitivity was sufficient to determine the aggregates and fragments in each individual IEC cut. The calculated total aggregates and fragments in the stressed mAb-1 samples were comparable to those detected by one dimensional SEC. Most importantly, the 2D IEC-SEC approach was capable of bridging the mAb charge and size variants in a real-time and efficient manner. It was observed that aggregates were enriched in the most basic region in the IEC charge profile, especially in the highly degraded samples. This is the first IEC-SEC 2D-LC application for intact antibody analysis as per the authors’ knowledge.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"105 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2017-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75694546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Optimization of Yield for Extraction of an Essential Oil from Cinnamon Using Microwave-Assisted Extraction 微波辅助萃取肉桂精油得率的优化研究

Journal of Chromatography & Separation Techniques Pub Date : 2017-05-01 DOI: 10.4172/2157-7064.S8-001

M. Satishkumar

{"title":"Optimization of Yield for Extraction of an Essential Oil from Cinnamon Using Microwave-Assisted Extraction","authors":"M. Satishkumar","doi":"10.4172/2157-7064.S8-001","DOIUrl":"https://doi.org/10.4172/2157-7064.S8-001","url":null,"abstract":"Essential oil is concentrated hydrophobic liquid which containing volatile aromatic compounds extracted from flowers, leaves, stems, roots, seeds, barks, resins, or fruit rinds. These oils are often used for their flavor and their therapeutic or odoriferous properties, in a wide selection of products such as foods, medicines, and cosmetics. Extraction of essential oils is one of the most time and effort consuming processes. Cinnamon is an important herbal medicine with good effects of health promotion and disease prevention. In this work, the volatile compounds of cinnamon bark were extracted by Hydrodistillation and Microwave-assisted extraction (MAE) techniques. Gas chromatography/mass spectrometry was used to identify and quantify the volatile compound composition. The results indicated that cinnamaldehyde was the major component with the highest % area of 89.324% in the volatile oils extracted by MAE while in hydrodistillation method cinnamaldehyde was about 68%. MAE has been recognized as a technique with several advantages over other extraction methods, such as reduction of costs, extraction time, energy consumption, and CO2 emissions. MAE technique is a green technique which is highlighted by increased extraction yield, decreased time and solvent consumption, moreover the reproducibility is better. For this purpose, various process parameters such as solid loading, water volume, microwave power and extraction time were studied in detail and optimized using the Taguchi method and Artificial neural network. The optimized extraction conditions were obtained at, solid loading of 30 g, water volume of 250 ml, microwave power of 700 W and extraction time of 25 min. Under optimized conditions, 4.169% (w/w) yield of essential oil was obtained.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"11 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2017-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86667906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Development and Validation of a Novel Stability Indicating UPLC Methodfor Dissolution Analysis of Bexarotene Capsules: An Anti Cancer Drug 抗肿瘤药物贝沙罗汀胶囊溶出度分析的新型稳定性指示UPLC方法的建立与验证

Journal of Chromatography & Separation Techniques Pub Date : 2017-04-28 DOI: 10.4172/2157-7064.1000364

V. Rao, K. Harikrishna

引用次数: 1

Application of Modified Clay for Sorption of Radionuclide Elements 改性粘土在放射性核素元素吸附中的应用

Journal of Chromatography & Separation Techniques Pub Date : 2017-04-10 DOI: 10.4172/2157-7064.1000363

Z. Liang

引用次数: 1

Efficient, Baseline Separation of Pyrethrins by Centrifugal PartitionChromatography 离心分离色谱法高效、基线分离除虫菊酯

Journal of Chromatography & Separation Techniques Pub Date : 2017-04-07 DOI: 10.4172/2157-7064.1000362

A. Wong, J. Gliński

{"title":"Efficient, Baseline Separation of Pyrethrins by Centrifugal PartitionChromatography","authors":"A. Wong, J. Gliński","doi":"10.4172/2157-7064.1000362","DOIUrl":"https://doi.org/10.4172/2157-7064.1000362","url":null,"abstract":"Pyrethrins are natural pesticides present in the oil extracted from the flowers of Chrysanthemum cinerariaefolium. They are potent ion-channel neurotoxins which have higher selective toxicity for insects compared to mammals. These phytochemicals have an exceptionally safe environmental profile and are an attractive natural alternative to organophosphate insecticides currently used in agriculture. The oil extract contains six pyrethrins compounds, which are structurally related esters. Isolation of reference standards for pyrethrins and their derivatives in multi-gram quantities is a prerequisite for studying toxicity and soil degradation for these natural pyrethrins. Chromatography is difficult because of the high hydrophobicity and close structural similarity. The isolated pyrethrin compounds also have poor stability because they are photosensitive. To facilitate these studies, we have developed a twostep purification process, in which the two groups were first separated by normal phase liquid chromatography on silica gel. The separation of pyrethrin, cinerin, and jasmolin within each group was achieved by centrifugal partition chromatography (CPC). We explored and successfully optimized solvent systems that were subsequently applied to achieve multi-gram scale separation. The pyrethrins I group compounds were separated using a solvent system containing heptane-methanol-acetonitrile (6:1:2, v/v) in ascending mode. The pyrethrins II group compounds were separated using a solvent system containing heptane-terbutylmethyl ether-acetonitrile-water (8:1:5:1.5, v/v) also in ascending mode. In both cases, gram quantities of individual pyrethrins with purity exceeding 99% were produced.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"44 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2017-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86783937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5