Journal of Chromatography & Separation Techniques最新文献

{"title":"Detection of Phytoconstituents of Medicinal Plant Terminalia arjuna Using Chromatographic Techniques","authors":"R. Chitte, Nagare Sl, Date Pk, S. Bp","doi":"10.4172/2157-7064.1000380","DOIUrl":"https://doi.org/10.4172/2157-7064.1000380","url":null,"abstract":"The plant extract revealed the presence of phytochemicals such as Phlobatannins, phenols, leucoanthocyanins, saponins, emodins, coumarins and quinones. Process of extraction of pure compound using column chromatography. The gradient of solvent eluted fraction has in pure form, tested and partial characterized. Thin layer chromatographic study was carried out by using various solvent system of varying polarity of which water, ethyl acetate and propanol system suited the best. In vitro anti-inflammatory activity was evaluated using albumin denaturation (50%) membrane stabilization assay (75%) and proteinase inhibitory activity (33.33%). For anti-inflammatory activity Aspirin (85.67%) used as standard drug. Using alfa amylase inhibition assay, In vitro antidiabetic activity was determined, fraction 5 (89.12%) and fraction 6 (80%) were showed at conc. 500 μg/mL. Antimicrobial efficiency of the plant extract fractions was determined using well diffusion method against Pseudomonas sp., Bacillus sp., Protease and Staphylococcus aureus, of which no activity was observed against Pseudomonas sp.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"19 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2017-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89589758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of high-performance liquid chromatography columns retentivity with the help of macromolecular probes","authors":"Dusan Berek","doi":"10.4172/2157-7064-C1-030","DOIUrl":"https://doi.org/10.4172/2157-7064-C1-030","url":null,"abstract":"","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"24 4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78323511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Method Establishment and Considerations of Ion Chromatography for Determination","authors":"Bing Qu, Shi-jun Su","doi":"10.4172/2157-7064.1000377","DOIUrl":"https://doi.org/10.4172/2157-7064.1000377","url":null,"abstract":"This paper provides some important technical points of ion chromatography for determination, including the necessity of simple operation, the choice of separating column and the importance of resolution, and so on. And the paper also introduces briefly the considerations and the problems how to deal with, such as eluent, tailing peak, precision, accuracy, and uncertainty. Thereby, it is necessary to develop new technologies for the application range of ion chromatography.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"82 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2017-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88985121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Notes on Synthesis of Perdeutero-5-13C,5,5,5-Trifluoroisoleucine VI56","authors":"Naugler Dg, Prosser Rs","doi":"10.4172/2157-7064.1000375","DOIUrl":"https://doi.org/10.4172/2157-7064.1000375","url":null,"abstract":"The 13CF3 group is a promising label for heteronuclear (19F,13C) NMR studies of proteins. Desirable locations for \u0000 this NMR spin label include the branched chain amino acid methyl groups. It is known that replacement of CH3 by \u0000 CF3 at such locations preserves protein structure and function and enhances stability. 13CF3 may be introduced at the \u0000 δ position of isoleucine and incorporated biosynthetically in highly deuterated proteins. This paper reports our work in \u0000 synthesis and purification of 5,5,5-trifluoroisoleucine, its perdeutero and 5-13C versions and of 2-13C-trifluoroacetate \u0000and its utility as a precursor for introduction of the 13CF3 group into proteins.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"151 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2017-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76850831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid Qualitative Detection of Rhodamine B in Chili Oil by Deep Eutectic Solvent Extraction and a Microplate Reader","authors":"W. Wang, Y. Du, Z. Xiao, Y. Li, B. Li, Yang Gw","doi":"10.4172/2157-7064.1000376","DOIUrl":"https://doi.org/10.4172/2157-7064.1000376","url":null,"abstract":"Rhodamine B, an alkaline industrial dye, was forbidden in food due to its potential carcinogenicity to humans. However, some unscrupulous merchants often used it as a food colouring agent to dope chili oil. In this research, a microplate reader was utilized for rapid qualitative detection of rhodamine B in chili oil with the deep eutectic solvent which was comprised of choline chloride and ethylene glycol (mole ratio 1:3) as an extraction medium.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"18 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2017-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81438806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehension of Effects of Intraparticle Effective Diffusivity on Simulated Moving Bed","authors":"Hong Gao","doi":"10.4172/2157-7064.1000371","DOIUrl":"https://doi.org/10.4172/2157-7064.1000371","url":null,"abstract":"The influence of intraparticle effective diffusivity on simulated moving bed (SMB) is investigated in this work. The intraparticle effective diffusivity has significant influence on the separation process of SMB and it affects the upper and left sides of separation region of SMB in different styles. The result is explained according to the separation of binary mixture in single column in C1-C2 space. This indicates the necessity of considering the effects of intraparticle mass transfer processes for chemical engineers during their designing the operating conditions of SMB.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"96 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2017-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81614926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of Allopurinol and Oxypurinol in Dogs Plasma by High- Performance Liquid Chromatography with an Ultraviolet Detector: Application for Pharmacokinetic Studies","authors":"Godoy Alpc, C. Dejesus, De Oliveira Ml, A. Rocha, Pereira Mpm, D. Larangeira, V. Lanchote, S. M. Barrouin-Melo","doi":"10.4172/2157-7064.1000373","DOIUrl":"https://doi.org/10.4172/2157-7064.1000373","url":null,"abstract":"High performance liquid chromatography with ultraviolet detection (HPLC-UV) was developed and validated for quantification of allopurinol and its active metabolite oxypurinol; in dog plasma with naturally acquired zoonotic visceral leishmaniasis. Allopurinol is a potent inhibitor of the xanthine oxidase enzyme; an enzyme that catalyzes the conversion of hypoxanthine to xanthine and from xanthine to uric acid. In veterinary medicine allopurinol is indicated in the treatment of canine visceral leishmaniasis. Allopurinol is utilized to inhibit the synthesis of Leishmania RNA. The conditions defined for development of the chromatographic analysis of dog plasma samples by utilizing the mobile phase of the HPLC-UV consist of a mixture of 0.1% water, 88% formic acid and 0.25% acetonitrile. Allopurinol and oxypurinol were separated on a LiChroCART® 125-4 LiChrospher® 100 RP-8 (5 μm) column utilizing a flow rate of 0.7 mL min-1 and detector operation was at a wavelength of 254 nm. Acyclovir was utilized as an internal standard. The validation of the HPLC-UV method was determined by limits of detection and quantification, linearity, reproducibility and repeatability. The method had a lower quantification limit of 0.1 μg.mL-1 for both allopurinol and oxypurinol. The analysis was linear at concentrations of 0.1-20.0 μg.mL-1 for both allopurinol and its oxypurinol. The precision value intracurrent and intercurrent for all concentrations presented had a coefficient variation lower than 15%. The confidence limits of HPLC-UV for analysis of allopurinol and oxypurinol in dog plasma indicates that the method is applicable to the multiple dose pharmacokinetic study of allopurinol in dogs. It is efficient, accurate and sensitive. This study of Pharmacokinetic research of allopurinol and oxypurinol in dogs with Stage I and II visceral leishmaniasis resulted in similar research outcomes that correlated with the healthy dog investigation.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"5 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2017-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87254222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Stoichiographic Methods for Separation and Determination of Components in Catalytic Processes","authors":"Malakhov Vv, Dovlitova Ls","doi":"10.4172/2157-7064.1000372","DOIUrl":"https://doi.org/10.4172/2157-7064.1000372","url":null,"abstract":"In this paper, the fundamental concepts of stoichiography is presented which open up a new area to determine \u0000 chemical composition, phase, molecular and ionic, of complicated mixtures of polyelement and polyphase inorganic substances. Doing it, there is no need to use standards of individual compounds and to get selective separation. \u0000 At the same time, a high possibility occurs to determine quantitatively chemical composition of the mixture occurs. \u0000 The general principles of two stoichiographic methods are set out: differential dissolution dedicated to analyze \u0000 mixtures of solid inorganic compounds; ionic chromato-stoichiography to analyze composition of water solutions. \u0000 Some examples are given concerning applications of the methods to analysis of heterogeneous and homogeneous \u0000catalysts.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84386144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The assessment of the exposure levels to mycotoxins among dairy cattle in the two South African provinces using HPLC (ESI)-MS/MSn","authors":"M. Makhafola, M. Coyanis","doi":"10.4172/2157-7064-C1-034","DOIUrl":"https://doi.org/10.4172/2157-7064-C1-034","url":null,"abstract":"","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83337455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The application of portable GC-MS on the petrochemical wharf","authors":"Zhao Yun","doi":"10.4172/2157-7064.1000374","DOIUrl":"https://doi.org/10.4172/2157-7064.1000374","url":null,"abstract":"It is very important to quickly identify the liquid chemicals in the petrochemical wharf when they volatile in the air. Since most of the liquid chemicals transported in the petrochemical wharf are harmful to people, and they spread very fast in the air. If the exact kind of liquid chemicals is undefined or is wrongly defined, it will lead to a big mistake in detection result. The mass spectrum is very effective in determining the gas kinds, but its big size and complex sampling mode are not convenient for quick detection. To resolve this problem, a method based on portable GC_MS for VOCs determination is developed. The VOCs are directly extracted by solid phase micro extraction head for 120 seconds. After that, they are analysed on the portable GC-MS, determined by retention time and MS peaks, quantified by external standard method. It only takes 6.4 min from sample extracting to get the result. And its linear correlation is larger than 0.98, and the minimum detectable concentration is lower than half of their occupational exposure limits. In the simulation test, the relative error is lower than 4.4%. This method can directly sample in the air, rarely depends on external conditions. At the same time, it can determine and quantify multiple kinds of VOCs at a time. So, it will be very suitable for the VOCs emergency detection in the leakage scene.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"10 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2017-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82888936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}