Journal of Chromatography & Separation Techniques最新文献

HPLC Method Validation for the Quantitative Fast Determination of 8 Organic Uv-Filters in Products with a Level of Sun Protection Factor Emulsion-Type 乳液型防晒系数产品中8种有机紫外线过滤剂的高效液相色谱法快速定量测定方法验证

Journal of Chromatography & Separation Techniques Pub Date : 2021-01-01 DOI: 10.35248/2157-7064.21.12.440

Juan David Marín Arevalo, Elizabeth Claros Ceballos, Alej, ra Patricia Guzmán Carantón, J. G. Galvis

{"title":"HPLC Method Validation for the Quantitative Fast Determination of 8 Organic Uv-Filters in Products with a Level of Sun Protection Factor Emulsion-Type","authors":"Juan David Marín Arevalo, Elizabeth Claros Ceballos, Alej, ra Patricia Guzmán Carantón, J. G. Galvis","doi":"10.35248/2157-7064.21.12.440","DOIUrl":"https://doi.org/10.35248/2157-7064.21.12.440","url":null,"abstract":"A fastHigh Performance Liquid Chromatography (HPLC) method was developed and validated for the simultaneous quantitative determination of phenylbenzimidazole sulfonic acid (PBMSA), benzophenone-3 (BZ3), diethylamino hydroxybenzoyl hexyl benzoate (DHHB), octocrylene (OC), butyl methoxydibenzoylmethane (BMBM), ethylhexyl methoxycinnamate (EMC), ethylhexyl salicylate (ES), and homosalate (HS) which are eightorganic UV-filters of the most commonly used in cosmetics and sunscreens. A solution pH 2.0 of phosphate buffer, methanol, and acetonitrile in proportions 15:3:82 respectively as mobile phase was used in isocratic conditions, combined with a Purospher Star ® Performance RP-18e column and eight minutes of analysis time. The proposed method was validated following the recommendations of International Council on Harmonization (ICH) and the Food and Drug Administration (FDA) guidelines. All parameters examined were found to be well within the stated guidelines. From the robustness test, it was possible to identify that pH of the buffer and the vial material are the critical methodological parameters for the analysis of butyl methoxydibenzoylmethane (BMBM), whereas for phenylbenzimidazole sulfonic acid (PBMSA) they were, the vial material, volume ofNaOH solution and sonication time and temperature.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"69 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78342462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial: Predictive Data on Atypical Femur FractureManuel DÃaz Curiel 社论:非典型股骨骨折的预测数据manuel DÃ-az Curiel

Journal of Chromatography & Separation Techniques Pub Date : 2021-01-01 DOI: 10.35248/2157-7064.21.12.E158

M. Curiel

引用次数: 0

Evaluation of Six Protocols for Protein Extraction from Rice Young Panicles byTwo-Dimensional Electrophoresis 二维电泳法提取水稻幼穗蛋白质的6种方法的评价

Journal of Chromatography & Separation Techniques Pub Date : 2021-01-01 DOI: 10.35248/2157-7064.21.12.438

Xiaoping Huang, Hui-wen Zhou

{"title":"Evaluation of Six Protocols for Protein Extraction from Rice Young Panicles byTwo-Dimensional Electrophoresis","authors":"Xiaoping Huang, Hui-wen Zhou","doi":"10.35248/2157-7064.21.12.438","DOIUrl":"https://doi.org/10.35248/2157-7064.21.12.438","url":null,"abstract":"ABSTRACT For most reported proteomics approaches, protein extraction and sample preparation are of crucial importance for optimal results. However, extraction of protein from crop plant tissues remains a great challenge due to low protein content and abundant secondary metabolites that prominently interfere with isoelectric focusing and subsequent proteomic analysis. Up until now, no attempts are focused on comparison of protein extraction procedures from rice young panicles. To establish a high-efficiency protein extraction protocol suitable for two-dimensional electrophoresis (2-DE) in rice young panicles, six protocols for protein preparation were evaluated: (1) Phenol extraction; (2) Mg/ Nonidet P-40 (NP-40) extraction; (3) Tris-Base/acetone precipitation; (4) SDS extraction; (5) trichloroacetic acid (TCA)/acetone/phenol extraction; (6) TCA/acetone precipitation. The study explicitly demonstrates that TCA/ acetone/phenol method provides a high-enhanced protein extraction efficacy from rice young panicles than other protocols in terms of the higher quality of 1-DE gel, the maximum number (450) of well-resolved protein spots, greater resolution and spot abundance. In addition, these methods also generated remarkably different twodimensional gel electrophoresis protein spot patterns. Twenty-nine of 30 visible differential extracted proteins were identified by MALDI-TOF-MS/MS analysis and were divided into eight categories according to molecular function. Accumulated data suggested that different extraction methods respectively necessitate certain special plant tissues due to different physicochemical properties of each protocol. Overall, this study, which is presented in this paper, will facilitate to providing a cornerstone of comparative proteomic analysis from rice young panicles, including other complicated plant tissues.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"120 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87798045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Improved Liquid Chromatographic Method for Determination of Aluminium in Sodium and Potassium Chloride Brine using Spectrophotometric Detection 改进的液相色谱法分光光度法测定氯化钠和氯化钾卤水中铝

Journal of Chromatography & Separation Techniques Pub Date : 2021-01-01 DOI: 10.35248/2157-7064.21.12.442

M. Uvaraj, A. Surendiran

{"title":"An Improved Liquid Chromatographic Method for Determination of Aluminium in Sodium and Potassium Chloride Brine using Spectrophotometric Detection","authors":"M. Uvaraj, A. Surendiran","doi":"10.35248/2157-7064.21.12.442","DOIUrl":"https://doi.org/10.35248/2157-7064.21.12.442","url":null,"abstract":"The quality of feed brine solution required for a modern chlor-alkali plant equipped with membrane cell is much more stringent. The electrical efficiency of the membrane is easily compromised by the presence of various anionic and cationic impurities [1]. Aluminium is one the impurity which will form alumino silicate precipitate when reacts with silicate. This will affect the membrane performance and sometimes even damages the membrane. Hence monitoring and controlling aluminium concentration is very crucial. There are many techniques and methods available for aluminium determination [2-5]. However many techniques fails to quantify aluminium concentration in trace level, especially in brine sample matrix. Chelation chromatography is one of the liquid chromatography techniques which can be used to quantify Aluminium in trace level using spectrophotometric detection [6-8]. The proposed method can be used to quantify trace level of aluminium in brine sample matrix. Aluminium forms complex with 8-hydroxy quinoline and detected using spectrophotometric detection method. This method does not require any derivatization.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"14 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88503617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amino Acid and Peptides Analysis Using Capillary Lc and Nano Lc: Food Analysis 氨基酸和多肽的毛细管Lc和纳米Lc分析:食品分析

Journal of Chromatography & Separation Techniques Pub Date : 2021-01-01 DOI: 10.35248/2157-7064.21.12.443

Jeremy R. Smith

引用次数: 0

Current Perspectives on Surgical Complications of Idiopathic Scoliosis 特发性脊柱侧凸手术并发症的研究现状

Journal of Chromatography & Separation Techniques Pub Date : 2021-01-01 DOI: 10.35248/2157-7064.21.12.447

H. Hamad

引用次数: 0

Simultaneous Determination of Amoxicillin, Clarithromycin and Esomeprazolein Mice Plasma after Oral Administration by Reverse Phase HPLC Method 反相高效液相色谱法同时测定小鼠口服阿莫西林、克拉霉素和埃索美拉唑的含量

Journal of Chromatography & Separation Techniques Pub Date : 2021-01-01 DOI: 10.35248/2157-7064.21.12.439

A. Mustafa, H. Ali, R. Bibi, A. Hassan, S. Khan, Khan Sa

{"title":"Simultaneous Determination of Amoxicillin, Clarithromycin and Esomeprazolein Mice Plasma after Oral Administration by Reverse Phase HPLC Method","authors":"A. Mustafa, H. Ali, R. Bibi, A. Hassan, S. Khan, Khan Sa","doi":"10.35248/2157-7064.21.12.439","DOIUrl":"https://doi.org/10.35248/2157-7064.21.12.439","url":null,"abstract":"A simple and precise reverse phase high-performance liquid chromatography (RP-HPLC) method for simultaneous determination of amoxicillin, clarithromycin and esomeprazole in mice plasma after an oral administration was developed and validated. An isocratic elution was achieved on C18 column with a mobile phase containing buffer, potassium di hydrogen phosphate (KH2PO4 ) 0.05 M at pH 5 and methanol (60:40 v/v) at a flow rate of 1 mL/min, injection volume was 10 µL and UV detection was kept at 205 nm. Linearity was in the range of 0.5-100 µg/mL for amoxicillin, 50-1000 µg/mL for clarithromycin and 0.1-100 µg/mL for esomeprazole. Limit of detection (LOD) and Limit of quantification (LOQ) were 0.26 µg/mL and 0.79 µg/mL for amoxicillin, 8.97 µg/mL and 27.20 µg/mL for clarithromycin, 0.13 µg/mL and 0.39 µg/mL for esomeprazole respectively. All samples were stable at room temperature for 72 hours. The pharmacokinetic studies showed that the maximum plasma concentrations (Cmax) were 1042.17 ± 4.0, 218.67 ± 5.3 and 18.97 ± 3.6 µg/mL for amoxicillin, clarithromycin and esomeprazole respectively. Whereas, the times to reach maximum plasma concentration (Tmax) were 2.0, 4.0 and 2.0 hours respectively. Over all, the validated HPLC method may be used for the determination of such drugs in their pharmaceutical formulation and can be applied for routine quality control analysis.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"1 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78165622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of Natural Dyes Extraction from Mangifera indica (Gadung) Leaves 芒果叶天然染料提取工艺的优化

Journal of Chromatography & Separation Techniques Pub Date : 2020-07-29 DOI: 10.21203/rs.3.rs-43734/v1

Ronaldo Pangestu Hadi, J. Valentina

{"title":"Optimization of Natural Dyes Extraction from Mangifera indica (Gadung) Leaves","authors":"Ronaldo Pangestu Hadi, J. Valentina","doi":"10.21203/rs.3.rs-43734/v1","DOIUrl":"https://doi.org/10.21203/rs.3.rs-43734/v1","url":null,"abstract":"\u0000 In recent years, due to the massive pollution spread, the switchover to the eco-friendly process is being considered. Particularly, in textile industries, the wastewater containing dangerous substances dangers the condition of the rivers directing to the use of natural dyes. The existing traditional industries often use natural sources with lacks of study about the method and process condition to gain the best quality and the highest quantity of colorants. This study aim is to find the time for the extraction using common materials and simple method resembling the real situation. The common natural dye source, Mangifera indica leaves, particularly from Gadung species – a common type of Mangifera indica in Indonesia – is on this study using reflux method combined with methanol and water as its solvent separately. The steps used is a modification from the preceding process, which includes tannin and fatty acids in the final product. Spectral analysis indicated that the best time for extraction using water, which produces pale yellow color is 60 min, and the best time for extraction using methanol, which produces green color is 360 min. Therefore, the next application of boiling the materials with water can be reduced to the written times above.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"41 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2020-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91163211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transforming Separation Science for Improved Human and Environmental Health and Addressing the Fundamental Challenges in Separation Science 转化分离科学，改善人类和环境健康，解决分离科学的基本挑战

Journal of Chromatography & Separation Techniques Pub Date : 2020-01-01 DOI: 10.35248/2157-7064.20.11.E146

Rabie Am

引用次数: 0

Determination of the Thermodynamic Behavior of a Therapeutic Peptide in Overloading Conditions in Gradient Elution Chromatography 在梯度洗脱色谱中测定一种治疗肽在超载条件下的热力学行为

Journal of Chromatography & Separation Techniques Pub Date : 2020-01-01 DOI: 10.35248/2157-7064.20.11.428

C. Luca, Simona Felletti, Giulio Lievore, Aless, R. Buratti, T. Chenet, L. Pasti, M. Morbidelli, A. Cavazzini, Martina Catani, M. Macis, Antonio Ricci, W. Cabri

引用次数: 1