Journal of Chromatography & Separation Techniques最新文献_第6页

A Presumable Mechanism of the Separation of Diastereomeric and Enantiomeric Mixtures 非对映异构体和对映异构体混合物分离的可能机理

Journal of Chromatography & Separation Techniques Pub Date : 2017-12-29 DOI: 10.4172/2157-7064.1000391

E. Pálovics, E. Fogassy

引用次数: 4

Analytical Method Development and Validation of Menthol and Methyl Salicylate Content in Topical Cream and Gel by Gas Chromatography 气相色谱法测定外用乳膏和凝胶中薄荷醇和水杨酸甲酯含量的分析方法的建立与验证

Journal of Chromatography & Separation Techniques Pub Date : 2017-12-18 DOI: 10.4172/2157-7064.1000390

K. Subhash, B. Bhavesh, Hemang

引用次数: 0

Evaluation of Driving Forces for Protein Partition in PEG-Salt Aqueous Two-Phase Systems and Optimization by Design of Experiments 聚乙二醇-盐两水相体系中蛋白质分配驱动力的评价及实验设计优化

Journal of Chromatography & Separation Techniques Pub Date : 2017-12-15 DOI: 10.4172/2157-7064.1000389

Anna Glyk, D. Solle, T. Scheper, S. Beutel

{"title":"Evaluation of Driving Forces for Protein Partition in PEG-Salt Aqueous Two-Phase Systems and Optimization by Design of Experiments","authors":"Anna Glyk, D. Solle, T. Scheper, S. Beutel","doi":"10.4172/2157-7064.1000389","DOIUrl":"https://doi.org/10.4172/2157-7064.1000389","url":null,"abstract":"In the present study, the partitioning of four model proteins (bovine serum albumin, ovalbumin, α-chymotrypsin and lysozyme) was investigated in polyethylene glycol (PEG)-salt aqueous two-phase systems (ATPS) addressing the effects of PEG molecular weight (MW) and concentration, phase-forming salt type (potassium phosphate/sodium citrate (KPP/NaCit)) and concentration, sodium chloride (NaCl) concentration and pH, as well as MW, isoelectric point, charge and hydrophobicity of the proteins. It was found that protein partitioning was influenced by different coexisting effects, such as hydrophobic and electrostatic interactions, related to the system parameters and physicochemical/surface properties of proteins. Moreover, a rigorous analysis and optimization of the significant forces for protein partitioning in PEG-salt ATPS was performed by design of experiments (DoE). Initially, KPP/NaCit, NaCl and PEG concentrations were considered as statistically significant factors by a fractional factorial design. Subsequently, the optimal values of the significant factors were determined by a central composite face-centered design coupled with response surface methodology. Furthermore, linear and quadratic models were obtained in the experimental designs, respectively, and evaluated by statistical regression analysis and analysis of variance. Finally, a validation of the quadratic model confirmed a good correlation between predicted and experimental results, thus verifying the validity of the model.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"11 1","pages":"1-16"},"PeriodicalIF":0.0,"publicationDate":"2017-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84307651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Micellar Liquid Chromatography Coupled with Fluorometric Detection for the Determination of Terbinafine in Dosage Forms and Plasma 胶束液相色谱-荧光法测定特比萘芬剂型和血浆中的含量

Journal of Chromatography & Separation Techniques Pub Date : 2017-11-27 DOI: 10.4172/2157-7064.1000388

Belal F, Sharaf El-Din MK, Eid Mi, El-Gamal Rm

{"title":"Micellar Liquid Chromatography Coupled with Fluorometric Detection for the Determination of Terbinafine in Dosage Forms and Plasma","authors":"Belal F, Sharaf El-Din MK, Eid Mi, El-Gamal Rm","doi":"10.4172/2157-7064.1000388","DOIUrl":"https://doi.org/10.4172/2157-7064.1000388","url":null,"abstract":"A newly developed micellar liquid chromatographic analysis technique was utilized for the determination of Terbinafine HCl (TRH). A 150 mm × 4.6 mm i.d., Shim-pack Cyanopropyl-bonded stationary phase was used for analysis. A mixture of 0.15 M sodium dodecyl sulphate (SDS), 0.3% triethylamine(TEA), 10% n-propanol all prepared in 0.02 M orthophosphoric acid was the mobile phase. The pH of the mobile phase was adjusted to 5.5 and pumped at a flow rate of 1 mL/min. The column temperature was set at 45°C. A fluorescence detection of (TRH) at 336 nm emission λ after excitation at 236 nm. Chlorzoxazone (CLZ) was used as an internal standard (IS). The method was linear over the range of 0.4-8.0 μg/mL. The limit of detection (LOD) and limit of quantification (LOQ) were 0.07 μg/ mL and 0.22 μg/mL, respectively. The method was further applied for analysis of the studied drug in its tablet, spray, cream and gel formulations. Not only this, but extending the application of the method for determination of terbinafine hydrochloride in spiked human plasma without prior extraction. Statistical analysis through comparison of the data obtained by the proposed and comparison methods, showed good accuracy and precision of the proposed method.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"1 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2017-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74061925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Purification and Biochemical Characterization of a Cystatin-Like Thiol Proteinase Inhibitor from Cicer arietinum (Chickpea) 鹰嘴豆中半胱抑素样硫醇蛋白酶抑制剂的纯化及生化特性研究

Journal of Chromatography & Separation Techniques Pub Date : 2017-11-07 DOI: 10.4172/2157-7064.1000387

S. Ab, Waseem Fb, S. Aaliya, Shahnawaz Km, Rizwan Hk, B. Bilqees

{"title":"Purification and Biochemical Characterization of a Cystatin-Like Thiol Proteinase Inhibitor from Cicer arietinum (Chickpea)","authors":"S. Ab, Waseem Fb, S. Aaliya, Shahnawaz Km, Rizwan Hk, B. Bilqees","doi":"10.4172/2157-7064.1000387","DOIUrl":"https://doi.org/10.4172/2157-7064.1000387","url":null,"abstract":"Cystatins are thiol proteinase inhibitors ubiquitously present in many plants as well as animal tissues. In the present study, a cystatin like a thiol proteinase inhibitor was isolated from chickpea using ammonium sulfate fractionation (40-60%) and gel filtration chromatography on a Sephacryl S-100 HR column with 21.09% yield and fold purification of 78.89. It gave a molecular mass of about 25.3 kDa as determined by SDS-PAGE, Dynamic Light Scattering (DLS), and from its gel filtration behavior. The inhibitor was found to have very low carbohydrate content (0.34%) and meager amount (1.45%) of sulfhydryl content. The Stokes radius, frictional, diffusion and sedimentation coefficients of CPC were 4.299 × 10-8 g/s, 22.82 A, 12.68 × 10-7 cm2 s-1 and 2.64 S, respectively. Kinetic analysis of CPC with thiol proteinases (papain, ficin, bromelain) revealed the reversible and competitive mode of inhibition with CPC showing the highest inhibitory efficiency against papain (Ki=0.82 nM) followed by ficin (Ki=17.6 nM) and then bromelain (Ki=41.3 nM). CPC possessed about 25.34% α-helical content as assessed by CD spectroscopy. UV-Visible and fluorescence spectra indicated that upon formation of papain-CPC complex there is a significant conformational change in one or both the proteins of the complex.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"14 1","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2017-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88775827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Quantifying Arsenic Metal in Drinking Water, Comments on: Highly Sensitive & Low Cost Colorimetric Method for Quantifying Arsenic Metalin Drinking Water of Malwa Punjab and Comparison With ICAP-AES by Sidhu et al. 饮用水中金属砷的测定——旁遮普省马尔瓦省饮用水中金属砷的高灵敏度、低成本比色法测定及与Sidhu等人的ICAP-AES比较

Journal of Chromatography & Separation Techniques Pub Date : 2017-10-28 DOI: 10.4172/2157-7064.1000386

Rathore Dps

引用次数: 1

Development of HPLC-UV Method for Detection and Quantification of Eight Organic Acids in Animal Feed 动物饲料中8种有机酸的HPLC-UV检测定量方法的建立

Journal of Chromatography & Separation Techniques Pub Date : 2017-10-23 DOI: 10.4172/2157-7064.1000385

Kim Hj, Lee Mj, Choubey Sk

引用次数: 5

Simultaneous Determination of Paracetamol, Aceclofenac and Chlorzoxazone in Pharmaceutical Dosage Form by UHPLC Method UHPLC法同时测定药品剂型中扑热息痛、乙酰氯芬酸和氯唑唑酮的含量

Journal of Chromatography & Separation Techniques Pub Date : 2017-10-11 DOI: 10.4172/2157-7064.1000384

P. Kb, Murugan, Ezhilarasan, Joseph

{"title":"Simultaneous Determination of Paracetamol, Aceclofenac and Chlorzoxazone in Pharmaceutical Dosage Form by UHPLC Method","authors":"P. Kb, Murugan, Ezhilarasan, Joseph","doi":"10.4172/2157-7064.1000384","DOIUrl":"https://doi.org/10.4172/2157-7064.1000384","url":null,"abstract":"Aim: UHPLC method was developed and validated for the determination of Paracetamol, Aceclofenac and Chlorzoxazone in pharmaceutical dosage form. Methods: Separation was performed in Agilent technologies 1220 infinity LC system equipped with open lab CDS Chemstation software using Eclipse plus C18 (50 × 4.6 mm id, 1.8 μm) column with mobile phase containing 0.05% orthophosphoric acid, adjusted to pH 6.0 with triethylamine and acetonitrile in the ratio of 70:30 v/v with a flow rate of 0.5 mL/min and detection wavelength 270 nm. Results: The method was found to be linear in the concentration range of 161.59-484.77 μg/mL for paracetamol, 49.69-149.09 μg/mL for aceclofenac and 124.81-374.43 μg/mL for chlorzoxazone with correlation coefficient of 0.999 for all the three drugs respectively. The %RSD of 0.57, 0.45 and 0.60 for intra-day and 0.96, 0.88 and 0.84 for inter-day precision, respectively for of Paracetamol, Aceclofenac and Chlorzoxazone suggest the precision of the method as all these values are less than 2%. The method has shown good, consistent recoveries for of Paracetamol (99.90-99.95%), Aceclofenac (99.83-99.92%) and Chlorzoxazone (99.39-99.59%) which are close to 100%. Conclusion: The method was found to be accurate, precise, specific, robust and linear for the determination of Paracetamol, Aceclofenac and Chlorzoxazone in pharmaceutical dosage form.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"38 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2017-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79398165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

An Attempt to Chromatographic Determination of Acceptor-Donor Properties of Liquid Crystals 液相色谱法测定液晶受体-供体性质的尝试

Journal of Chromatography & Separation Techniques Pub Date : 2017-09-29 DOI: 10.4172/2157-7064.1000383

H. Grajek, M. Purchała

引用次数: 2

Screening of Polyphenol Composition and Antiradical Capacity of Wild Erica arborea: A Native Plant Species from the Portuguese Flora 葡萄牙原生植物野生埃里卡多酚组成及抗自由基能力的筛选

Journal of Chromatography & Separation Techniques Pub Date : 2017-09-05 DOI: 10.4172/2157-7064.1000379

A. Aires, R. Carvalho

{"title":"Screening of Polyphenol Composition and Antiradical Capacity of Wild Erica arborea: A Native Plant Species from the Portuguese Flora","authors":"A. Aires, R. Carvalho","doi":"10.4172/2157-7064.1000379","DOIUrl":"https://doi.org/10.4172/2157-7064.1000379","url":null,"abstract":"With this study, we aimed to determine the polyphenol composition and antiradical activity of activity of Erica arborea a native plant species from the Portuguese and Mediterranean flora and often reported as having important bioactivities, but without consistent scientific evidences. The analysis of polyphenol profile and content was performed by HPLC-DAD/UV-Vis and in vitro bioassay of 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+) method was used to evaluate the radical scavenging activity. The HPLC analysis showed a great diversity of compounds. E. arborea presented high contents in flavonols (52%) and flavanols (25%) and the average content of antiradical activity higher than 85%. The polyphenols identified are often associated by literature as having preventive properties and protective effect against of degenerative and inflammatory processes, thus, our findings confirm the empirical health properties often associated to this endemic plant. Therefore, based in our study the E. arborea, can be used to extract and purify phytochemicals with potentially beneficial effects on health.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"19 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2017-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84593213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2