Journal of Chromatography & Separation Techniques最新文献_第5页

New Developed UHPLC Method for Selected Urine Metabolites 新型UHPLC法测定尿液代谢物

Journal of Chromatography & Separation Techniques Pub Date : 2018-01-01 DOI: 10.4172/2157-7064.1000404

M. Valko-Rokytovská, P. SalayováAOčenáš, Z. Kostecká

引用次数: 7

Development and Validation of an Innovative and Ecological Analytical Method Using High Performance Liquid Chromatography for Quantification of Cephalothin Sodium in Pharmaceutical Dosage 高效液相色谱法定量头孢菌素钠的创新生态分析方法的建立与验证

Journal of Chromatography & Separation Techniques Pub Date : 2018-01-01 DOI: 10.4172/2157-7064.1000394

Aleixa do Nascimento P, Kogawa Ac, Salgado Hrn

{"title":"Development and Validation of an Innovative and Ecological Analytical Method Using High Performance Liquid Chromatography for Quantification of Cephalothin Sodium in Pharmaceutical Dosage","authors":"Aleixa do Nascimento P, Kogawa Ac, Salgado Hrn","doi":"10.4172/2157-7064.1000394","DOIUrl":"https://doi.org/10.4172/2157-7064.1000394","url":null,"abstract":"Cephalothin is a first-generation cephalosporin, that shows great activity against Gram-positive microorganisms. Its effect is bactericidal, and due its action, it is the most efficient first-generation cephalosporin against resistant microorganism (β-lactamase producers). Although this drug has been clearly studied and researched about its antimicrobial activity, pharmacokinetics and pharmacodynamics, there are a few studies in literature regarding the development of analytical methodology for this cephalosporin. The aim of this work was to develop and validate a new method of analysis, using high performance liquid chromatography, resulting in an innovative method, quick and using solvents of low toxicity, minimizing, in this way, its toxic actions to the operators and leavings in the environment. The method developed and validated for the quantification of sodium cephalothin in lyophilized powder for injectable solution used high performance liquid chromatography (HPLC). The mobile phase consisted in water with 0.7% of glacial acetic acid and ethanol (70:30 v/v), wave-length of 237 nm, Zorbax Eclipse Plus C18 AgilentTM column and room temperature of 25°C, retention time of 4,20 minutes. The method was linear in the concentrations of 20, 40, 60, 80 e 100 μg/mL, selective, accurate and robust towards these modifications: ethanol brand, water source, mobile phase rate, glacial acetic acid proportion, flow rate, room temperature and wave length. The dosing for CET was of 106.72%.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"13 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85698255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Chemical Characterization and Cytotoxic Potential of a Chloroform Fraction Obtained from Marine Plant Thalassia testudinum 海洋植物海参氯仿组分的化学性质及细胞毒性研究

Journal of Chromatography & Separation Techniques Pub Date : 2018-01-01 DOI: 10.4172/2157-7064.1000405

M. Riera-Romo, D. MarreroDelange, I. Hernández-Balmaseda, K. González, D. Pérez-martínez, A. Manso-Vargas, M. Labrada, I. RodeiroGuerra, W. Vandenberghe

引用次数: 3

Development and Validation of RP-HPLC Method for Simultaneous Determination of Diclofenac Potassium and its Process Related Impurities in Solid Oral Dosage Form 固体口服剂型中双氯芬酸钾及其工艺相关杂质的反相高效液相色谱测定方法的建立与验证

Journal of Chromatography & Separation Techniques Pub Date : 2018-01-01 DOI: 10.4172/2157-7064.1000412

Thirupathi Dongala, A. K. Palakurthi, Kiran Kumar Velaveni, N. Katari

{"title":"Development and Validation of RP-HPLC Method for Simultaneous Determination of Diclofenac Potassium and its Process Related Impurities in Solid Oral Dosage Form","authors":"Thirupathi Dongala, A. K. Palakurthi, Kiran Kumar Velaveni, N. Katari","doi":"10.4172/2157-7064.1000412","DOIUrl":"https://doi.org/10.4172/2157-7064.1000412","url":null,"abstract":"The paper reports an efficient and rapid fast LC method for estimation of diclofenac potassium and its impurities. The main aim of the study is to develop and validate a simple, rapid, accurate, sensitive, less time consuming and less expensive method by using RP-HPLC with UV detector. The chromatographic separation of diclofenac potassium and its impurities was carried out by using 150 × 4.6 mm, i.d., 5 μm C-18 column with prepared mobile phase-A consisting 800:200 (v/v) of 0.01 M ammonium acetate adjusted pH 5.3 with acetic acid and acetonitrile and mobile phase-B consisting 200:800 (v/v) of 0.01 M ammonium acetate adjusted pH 5.3 with acetic acid and acetonitrile. The wavelength for detection of were made at 280.0 nm using UV detector. The flow rate of 1 mL/min. The method was able to detect and separate impurities and diclofenac potassium. The system suitability parameters were found within the limits. The coefficient of correlation for diclofenac potassium and its impurities was found not less than 0.998. The %recovery was found within the ICH limits. The LOD and LOQ values from study demonstrate method is sensitive. The method was validated for linearity, precision, accuracy, specificity, intermediate precision, ruggedness, robustness, stability and suitability.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"36 ","pages":"1-15"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91554367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Simultaneously Determination of Fifteen Estrogens and Metabolites in Human Serum by an Efficient Liquid Chromatography-Tandem Mass Spectrometry Method 高效液相色谱-串联质谱法同时测定人血清中15种雌激素及其代谢物

Journal of Chromatography & Separation Techniques Pub Date : 2018-01-01 DOI: 10.4172/2157-7064.1000407

Chang Cheng, J. Hou, Sheng Wang, Bi-xue Xu, Shao-Feng Liu, Zimeng Yan

{"title":"Simultaneously Determination of Fifteen Estrogens and Metabolites in Human Serum by an Efficient Liquid Chromatography-Tandem Mass Spectrometry Method","authors":"Chang Cheng, J. Hou, Sheng Wang, Bi-xue Xu, Shao-Feng Liu, Zimeng Yan","doi":"10.4172/2157-7064.1000407","DOIUrl":"https://doi.org/10.4172/2157-7064.1000407","url":null,"abstract":"Determination of female hormones and their metabolites in human serum is one of the most critical steps in human physiological and pathological diagnosis, such as in risk assessment for certain cancers. There are a number of concerns on sample derivatization, method specificity, sensitivity, separation efficiency and long elution time, although a large number of methods using Liquid Chromatography-Mass Spectrometry (LC-MS) and Gas Chromatography-Mass Spectrometry (GC-MS) for determination of estrogens and metabolites have been published before. We successfully developed and validated an efficient Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) method for determination of fifteen estrogens and metabolites in human serum. The estrogens and metabolites were derivatized with dansyl chloride and analyzed by LC-MS/MS using positive Electrospray Ionization (ESI+) and a Multiple Reaction Monitoring (MRM) mode. A Phenomenex Synergi Hydro-RP 2.5 μm 80 Å column (100 × 2.0 mm) at 60°C was eluted with mobile phases consisting of acetonitrile, methanol, water and formic acid at a flow rate of 0.4 mL/min. All of the fifteen dansyl derivatives of estrogen and metabolites were separated by LC with retention time differences between peaks ≥ 0.2 min. The recoveries of the fifteen estrogens and metabolites from hydrolysis and derivatization were in a range of 74.4-95.6% at 30-801 pg/mL level. The interday accuracies of the dansyl derivatives were in a range of 91.7-109.6% with a precision of 2.7-12.6%RSD. The LC-MS/MS system had linear responses to these derivatives within the calibration range of 12-10980 pg/mL with regression coefficient r2 ≥ 0.9934. The Limits of Quantification (LOQ) of these derivatives were in a range of 5.3-71.1 pg/mL in human serum. In comparison to a typical published method, our method provided a more efficient LC separation using only one-third of the elution time, and a similar or slightly better sensitivity.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78598367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Evaluation of an ion chromatography method for quantitating sulfate in plasma, serum and urine 离子色谱法测定血浆、血清和尿液中硫酸盐含量的评价

Journal of Chromatography & Separation Techniques Pub Date : 2018-01-01 DOI: 10.4172/2157-7064.1000411

P. Dawson, A. McWhinney, M. Reimer, F. Bowling

引用次数: 2

Optimization of Headspace Solid-Phase Microextraction and Gas Chromatography Coupled with Mass Spectrometry Technique for the Quantification of Volatile Compounds in a Fresh Cheese (Oaxaca) 顶空固相微萃取-气相色谱-质谱联用技术测定瓦哈卡奶酪挥发性成分的优化

Journal of Chromatography & Separation Techniques Pub Date : 2018-01-01 DOI: 10.4172/2157-7064.1000415

S. JoséRogelio, oval Copado, J. O. Villafuerte, M. M. G. Fabila, Maria de los Angeles Colin Cruz

{"title":"Optimization of Headspace Solid-Phase Microextraction and Gas Chromatography Coupled with Mass Spectrometry Technique for the Quantification of Volatile Compounds in a Fresh Cheese (Oaxaca)","authors":"S. JoséRogelio, oval Copado, J. O. Villafuerte, M. M. G. Fabila, Maria de los Angeles Colin Cruz","doi":"10.4172/2157-7064.1000415","DOIUrl":"https://doi.org/10.4172/2157-7064.1000415","url":null,"abstract":"The Head Space Solid Phase Microextraction Method (HS-SPME), coupled with Gas Chromatography and Mass Spectrometry (GC-MS) was optimized to quantify Volatile Organic Compounds (VOC’s) in a fresh Oaxaca cheese during its shelf life of 22 days. Optimal extraction conditions were ascertained by means of a multiple linear regression analysis and the generation of a response surface. Conditions to reach the equilibrium of VOC’s at the headspace were established to be 30 minutes of ultrasonic agitation at 50°C. The conditions for extraction were exposure of the fiber at room temperature for 30 minutes. Optimal extraction of volatile compounds released by the Oaxaca cheese allowed identification of 21 volatile compounds: 8 acids, 1 alcohol, 4 aldehydes, 4 ketones, 1 ester, and 3 lactones. Quantification of VOC’s was carried out using the technique of standard addition. The limits of quantification ranged from 0.0033 to 0.0311 ng/μg. Ten compounds were identified with the heaviest concentration and are listed as follows, from heaviest to lightest: propionic acid, acetoin, acetic acid, diacetyl, capric acid, caprylic acid, δ-decalactone, δ-dodecalactone, γ-dodecalactone, butyraldehyde. During storage of the cheese, the most significant changes in the concentration of VOC’s took place between day 1 and day 15. Based on the results obtained, it can be concluded that the optimization of the SPME makes it possible to isolate and quantify VOC’s of fresh cheeses in an efficient and reproducible manner.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"47 6 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83549803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Development and Validation of Method for Bio-Quantification of Marbofloxacin in Sheep Plasma by Liquid Chromatography Tandem Mass Spectrometry 液相色谱串联质谱法测定绵羊血浆中马布沙星生物定量方法的建立与验证

Journal of Chromatography & Separation Techniques Pub Date : 2018-01-01 DOI: 10.4172/2157-7064.1000408

Patel Hb, Mody Sk, Gondaliya Sb, Singh Rd

引用次数: 0

Detection of Synthetic Anti-Obesity Drugs, Designer Analogues and Weight- Loss Ingredients as Adulterants in Slimming Foods from 2015 to 2017 2015 - 2017年减肥食品中合成抗肥胖药物、设计类似物和减肥成分的掺假检测

Journal of Chromatography & Separation Techniques Pub Date : 2018-01-01 DOI: 10.4172/2157-7064.1000396

J. Yun, J. Choi, J. C.H., K. Kwon

{"title":"Detection of Synthetic Anti-Obesity Drugs, Designer Analogues and Weight- Loss Ingredients as Adulterants in Slimming Foods from 2015 to 2017","authors":"J. Yun, J. Choi, J. C.H., K. Kwon","doi":"10.4172/2157-7064.1000396","DOIUrl":"https://doi.org/10.4172/2157-7064.1000396","url":null,"abstract":"Obesity is a chronic disease associated with serious health problem such as metabolic syndrome, diabetes, hypertension, and cardiovascular disease. Recently, anti-obesity drugs, designer analogues and natural weight-loss ingredients have been found in dietary supplements for rapid weight loss or maintenance. In addition, structurally modified designer analogues are being continuously synthesized to avoid governmental inspections. 25 illegal compounds were assayed using a liquid chromatography coupled with photodiode array detector (LC-PDA) and tandem mass spectrometry (UPLC-MS/MS) analyses. A simultaneous determination method was established and fully validated. In 76 of 370 samples, nine different adulterants were detected including yohimbine, followed by β-phenylethylamine (β-PEA), sibutramine, sennoside A and B, β-methyl phenylethylamine (BMPEA), fluoxetine, ephedrine, phenolphthalein, and icariin. And 12 adulterated products contained a mixture of two or more adulterants. Adequate use of the existing regulation and the development of alternative methods for detecting adulterants are necessary consumers from consuming dietary supplements adulterated with illegal compounds.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"17 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87176998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Removal of Hexavalent Chromium (Cr6+) Using Different Natural Adsorbents - A Review 不同天然吸附剂去除六价铬(Cr6+)研究进展

Journal of Chromatography & Separation Techniques Pub Date : 2017-12-30 DOI: 10.4172/2157-7064.1000392

Yogeshwaran, Priya Ak

{"title":"Removal of Hexavalent Chromium (Cr6+) Using Different Natural Adsorbents - A Review","authors":"Yogeshwaran, Priya Ak","doi":"10.4172/2157-7064.1000392","DOIUrl":"https://doi.org/10.4172/2157-7064.1000392","url":null,"abstract":"Heavy metal is one of the major environmental and ecological problems in this world. The presence of heavy metals in water and wastewater causes toxic effects to the living beings and the environment. Compared to other heavy metals (such as Cr6+, Pb2+, Zn2+ etc.,) Chromium found in the tannery and electroplating industrial effluents causes serious effects to the humans and living organisms. It also contaminates the soil and groundwater. The excess amount of chromium affects the lungs and lead to respiratory disorders in the human beings. Due to a large number of tanneries and electroplating industries, the chromium contamination in the wastewater and aqueous solutions exceeds the tolerance limits. Many methods are used to remove the chromium from the aqueous solutions and industrial effluents. Adsorption is one of the cost - effective methods being widely used for the removal of heavy metals from industrial and commercial wastewaters. It has been established as an important and economically feasible treatment technology for removing heavy metals, particularly chromium. This review article presents the information about the application of different kinds of natural waste adsorbents for the removal of hexavalent chromium (Cr6+) from industrial effluent.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"213 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2017-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77503185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18