Development and Validation of Method for Bio-Quantification of Marbofloxacin in Sheep Plasma by Liquid Chromatography Tandem Mass Spectrometry

Abstract

A simple, time saving, and sensitive liquid chromatography tandem mass spectrometric method has been developed and validated for determination of marbofloxacin from sheep plasma. Marbofloxacin was extracted from sheep plasma by liquid-liquid extraction. Detection was performed as transition of marbofloxacin into two product ions i.e., 363.00→320.00 and 363.00→245.00 using mass spectrometry. Chromatographic separation was performed using reverse phase C18 column at flow rate of 0.2 mL/min. The assay of marbofloxacin was linear over the range of 0.001 μg/mL to 10 μg/mL with regression co-efficient (r2)>0.9994. The observed value of intra-day and inter-day precision of assay was found between 5.196 to 10.686% and 2.343 to 13.768%, respectively. The mean percent recovery of marbofloxacin from sheep plasma varied from 74.91 to 78.90%. The assay was used successfully for pharmacokinetics and bioavailability studies of marbofloxacin in sheep.