Journal of Cytology and Histology最新文献

{"title":"Effect of Lentil and Buffalo Whey Protein Hydrolysates on Histopathology of Liver and Kidney in Diabetic Rats","authors":"G. Bisar, M. Youssef, K. E. Saadany, W. El-Kholy, E. Kheadr","doi":"10.4172/2157-7099.1000489","DOIUrl":"https://doi.org/10.4172/2157-7099.1000489","url":null,"abstract":"This study was undertaken to evaluate the effects of protein hydrolysate prepared from lentil and buffalo whey proteins on body weight and histopathology of alloxan induced diabetic rats. Seventy two male albino rats were randomly divided into non-diabetic, diabetic untreated and diabetic treated with lentil or buffalo whey protein hydrolysates with or without yogurt. Experimental diabetes was induced by a single intraperitoneal (i.p) administration of alloxan (120 mg/kg body weight). Histopathological analyses were conducted on the liver and kidney tissues after 28 day of experiment. While the body weight was significantly decreased in the diabetic control group, there were no significant difference in diabetic treated group (P<0.05). Destruction of the liver architecture of the hepatocytes in the diabetic group showed the signs of steatosis, fibrosis and inflammation in the central vein and blood vessels. In the kidney, intestinal nephrites and mesangial proliferation and formation of cast material were observed. Yoghurt-containing lentil protein hydrolysate contained in yogurt appeared to have remarkable effect to prevent damage in liver and kidney histology caused by diabetes.","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"8 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2017-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79233885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Case of Radiation Induced Osteosarcoma of Proximal Humerus in Undifferentiated Nasopharyngeal Carcinoma","authors":"L. Amaadour, H. Ouahbi, Z. Benbrahim, Fatimazahra El’mrabet, S. Arifi, N. Mellas","doi":"10.4172/2157-7099.1000490","DOIUrl":"https://doi.org/10.4172/2157-7099.1000490","url":null,"abstract":"Radiation-induced osteosarcomas (RIOS) after nasopharyngeal carcinomas are rare complication of radiotherapy and are associated with poor prognosis. Few cases are reported in the literature. Case report: We report a case of radiation-induced osteosarcoma involving the proximal humerus in a patient treated with radiotherapy for nasopharyngeal carcinoma (NPC) nine years ago. Surgical treatment could not be performed, and the patient received induction chemotherapy. He died from disease spread after three cycles. Conclusion: RIOS after treatment of NPC is very aggressive complication. Only a regular follow up of treated patient allow early detection of these tumors and best chances of survival.","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"114 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2017-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88127512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surgical Management of a Case of Bilateral Auricular Keloids in an Adolescent Girl","authors":"P. Jeyaraj, L. Sehgal","doi":"10.4172/2157-7099.1000491","DOIUrl":"https://doi.org/10.4172/2157-7099.1000491","url":null,"abstract":"The Keloid is a fibroproliferative anomaly of the cutaneous connective tissue secondary to dysregulation in the skin healing and repair process, occurring in susceptible individuals. It is characterized by excessive collagen and glycoprotein deposition in the dermis following any local irritation, inflammation, burn, incision or injury, thereby leading to a cosmetically unaesthetic, aberrant and exuberant scar formation extending well beyond the boundaries of the original wound. It usually presents as firm nodules, often pruritic and painful, which do not regress spontaneously. The condition presents quite a therapeutic challenge owing to its unpredictably aggressive nature, frequent invasion of adjacent normal dermis, occasional appearance of satellite lesions in nearby non-traumatized tissue and a remarkable tendency for recurrence following removal. \u0000Various combinations of Pressure therapy, intralesional steroid therapy and surgery have shown promising results in the treatment of auricular keloids. We report on a young Indian female, aged 19 years, who developed bilateral auricular keloids subsequent to earlobe piercing, which was successfully managed by careful and atraumatic surgical excision alone.","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"1 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2017-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85791544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fitted Pressure Garment of Assessment of Scar Thickness on Third-Degree Burns through Ultrasonic Measurement","authors":"Pi-Wen Huang, Chih-Wei Lu, Hsin Liang Liu","doi":"10.4172/2157-7099.1000488","DOIUrl":"https://doi.org/10.4172/2157-7099.1000488","url":null,"abstract":"The aim of this study was to assess the thickness of the scars on the left arm of a female third-degree burn patient through ultrasonic examination in order to investigate the use of 25-mm Hg pressure garments, which are a key treatment for burns. The patient was injured with third-degree burns more than 10 years ago. Sixteen scars were examined. The resulting Pearson correlation of the forearm and upper arm was 0.138 (weak correlation; R2=0.0191). Therefore, in this case, all data points fell on a straight line with a positive (upward) slope. This result indicated different thicknesses for the upper arm and forearm third-degree burn scars; this result displays different thickness for the upper arms and forearm the third degree burn scars; the scars did not spread evenly due to the loading capacity of different parts of body instead of the pressure by pressure garment.This paper provides accurate information to manufacturers, therapists, and tailors regarding measurements for pressure garments. The results suggest that burn scars should be subject to appropriate pressure on the basis of medium scar height to avoid increasing scars and to stabilise burns, validating the hypothesis that 25 mmHg is not the optimal pressure for female patients with third-degree burns on the left arm and that the optimal pressure should be based on the patient’s burns and wound area. To our knowledge, this is the first study to correlate the thickness of third-degree burns through ultrasound scan.","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"7 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2017-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87319388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Opinion on Different Findings in Experimental and Clinical Medicine","authors":"F. Werner, R. Coveñas","doi":"10.4172/2157-7099.1000487","DOIUrl":"https://doi.org/10.4172/2157-7099.1000487","url":null,"abstract":"In this paper we are giving a brief account on Volume 8 Issue 4, it contains different opinions, research articles, case reports and minireviews about experimental and clinical themes and about dementia. Singh wrote the following editorial entitled: “Oxidative Stress and Neurodegeneration”. Oxidative stress is responsible for the death of microglia and astrocytes and this is due to an imbalance between production and deactivation of reactive nitrogen species and reactive oxygen species.","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"30 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2017-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81097326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diabetic Mastopathy, Diagnoses and Management","authors":"H. Ragmoun, A. Ajili, A. Daadoucha","doi":"10.4172/2157-7099.1000485","DOIUrl":"https://doi.org/10.4172/2157-7099.1000485","url":null,"abstract":"Diabetic mastopathy (DM) is a pathological entity of recent description, it is rare and benign; occurring in young patients with type I diabetes, or having an autoimmune disease. Clinically, she simulates breast cancer. Radiological examinations are not very informative. The diagnosis of certainty is histological. Through an observation of (DM) occurred in a diabetic patient, under insulin for 14 years and carrying multiple degenerative complications, we recall the clinical aspects, radiological and evolutionary of this affection","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"123 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2017-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75274533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of Wound TGF Beta-1 by Celecoxib: A Possible Therapeutic Route for Scar Free Wound","authors":"S. A. El-Aleem, E. Jude","doi":"10.4172/2157-7099.1000481","DOIUrl":"https://doi.org/10.4172/2157-7099.1000481","url":null,"abstract":"Background: Wound healing is a highly ordered dynamic process associated with inflammation at early stages and with permanent scarring at late stages. Scars could be disfiguring and could advance to be hypertrophic or keloid scars, this would have a strong physical and psychological impact on the patients afterward. The role of inflammatory mediators which could be pro- or anti-inflammatory, pro-or antifibrotic was the focus of wound healing research for decades and the balance between them is the key factor determining the outcome of healing.Aims: In this study, we investigate the correlation and the interrelation between the pro-inflammatory Cyclooxygenase-2 (COX-2) and the pro fibrotic (TGF-Beta-1) in an in vivo model of incisional dermal wound healing and the effect of a selective COX-2 inhibiton the progression of repair and scar formation.Materials and methods: Adult male Sprague-Dawley rats received four full thickness dermal wounds. a selective COX-2 inhibitor was applied to the wounds immediately postwounding twice daily for two days. Wounds and scars were then harvested and at different time points and processed for COX-2 and TGF Beta-1 immunostaining and for collagen staining. Immunoreactivity was semi quantified using Image J.Results: We have shown upregulation of COX-2, co-upregulation and colocalization of TGF-Beta-1 and COX-2 two days postwounding during the inflammatory phase. Celecoxib application significantly inhibited both COX- 2 (P<0.01) and TGF Beta-1 (P<0.001). It improved wound healing microscopically and macroscopically, through reducing inflammatory cell infiltrate, granulation tissues formation and early closure of the incision. Additionally, there was marked improvement in the postwounding scarring. There was a significantly (P<0.01) correlation between COX-2 and TGF Beta-1 (Pearson Correlation=0.94).Conclusion: The overall effect of COX-2 inhibitor was shortening of the inflammatory phase of wound healing with subsequent minimization of the associated tissue destruction and consequently improvement of the scar quality. COX-2 inhibitors regulate inflammatory phase of the wound. They could regulate collagen deposition byregulating the produc-tion of the pro fibrotic TGF Beta-1 production, through autocrine/paracrine effect. Therefore, early application of COX-2 inhibitors to wounds immediately after injury/surgery could enhance the repair and improve the quality of scar.","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"1 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2017-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82071483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Osteosarcoma in Dogs: Diagnosis and Treatment - A Literature Review","authors":"Paula de Sanctis, B. Caetano, L. M. M. Flórez, V. B. D. Souza, Willian Fern, O. Zambuzzi, N. Rocha","doi":"10.4172/2157-7099.1000473","DOIUrl":"https://doi.org/10.4172/2157-7099.1000473","url":null,"abstract":"Among primary bone tumors, osteosarcoma (OSA) is the most diagnosed in dogs. OSA is a highly aggressive tumor and metastatic spread to the lungs contributes to the poor prognosis. Surgery followed by adjuvant chemotherapy and radiation therapy is the current treatment of choice for this disease. However, surgery is not applied in all cases because dog owners disagree with partial or total amputation of the limb. Due to the aggressiveness of the disease, this review aims to gather information about the biological bases of the OSA, to discuss its diagnosis and treatment as well as to serve as an instrument in comparative oncological research encouraging effective diagnostic and therapeutic approaches to be improved and discovered.","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"61 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2017-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84019751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-upregulation of Transforming Growth Factor Beta-1 and Nitric Oxide Synthase in Keloid by comparison to normal human skin-A possible role for TGFβ1 and NOS in pathogenesis of keloid","authors":"S. A. El-Aleem, Soha Abdelwahab, N. M. Osman","doi":"10.4172/2157-7099.1000474","DOIUrl":"https://doi.org/10.4172/2157-7099.1000474","url":null,"abstract":"Keloid disease is a benign but progressive form of abnormal wound healing associated with skin fibrosis and can cause a major functional disability and morbidity. TGF beta (TGFβ) and Nitric Oxide (NO) are active biomarkers known to regulate phases of wound healing and have been implicated in pathogenesis of fibrotic disease. There are three isoforms of TGFβ (1, 2 and 3), TGFβ1 and 3 have a crucial role in fibrosis, with TGFβ1 profibrotic and TGFβ3 antifibrotic. NO is produced by Nitric Oxide Synthase (NOS) which exist in three isoforms, inducible NOS (iNOS), endothelial NOS (ecNOS) and neuronal NOS (nNOS). TGFβ isoforms and NO were found to be associated with fibrotic disorders affecting the skin. We hypothesis that the interaction between TGFβ and NO in keloid could promote the excessive collagen deposition associated with this disorder. Using immunohistochemistry, we investigated the profile of TGFβ isoforms (TGFβ1, 3) and NOS isoforms (iNOS and ecNOS) in keloid tissues and normal human skin. The cellular distribution of all the isoforms were studied and the protein levels were assessed by using H-Scoring and Image J Scoring systems. TGFβ1 showed wide cellular distribution in keloid both in the epidermal and dermal cells. There was significant upregulation (P<0.0001) by comparison to normal skin. TGFβ3 showed limited expression in keloid and there was significant downregulation (P<0.03). iNOS and ecNOS showed significant upregulation in keloid by comparison to normal skin (P<0.01 and P<0.02) respectively. Interestingly, iNOS was expressed in the basal epidermal layer and in dermal connective tissue cells while ecNOS was solely expressed in vascular endothelial lining. Although it is documented that TGFβ has a negative feedback effect on iNOS, we have shown co-upregulation of TGFβ1 and iNOS in keloids. Thus, in keloid NO is as important as the profibrotic growth factor TGFβ1 and both could be working in coordination. Moreover, the lack of effective therapy for keloid could be because most of the therapeutic regimen target one factor while the other still in action. In conclusion, understanding the actions of TGFβ and NOS in keloid disease could lead to the development of clinically useful combined anti-fibrotic agents.","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"148 Pt 7 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2017-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84084722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation between Epiretinal Membrane Bridging, Visual Acuity andCentral Macular Thickness","authors":"J. Paović, P. Paovic","doi":"10.4172/2157-7099.1000471","DOIUrl":"https://doi.org/10.4172/2157-7099.1000471","url":null,"abstract":"Epiretinal membrane or macular pucker is avascular, fibrocellular membrane that develops on the inner surface of the retina that can result in various stages of macular dysfunction. Fine epiretinal membranes lead to minimal visual loss, whilst epiretinal membranes (that may or may not bridge the fovea) are, in their more severe stages, accompanied by macular edema and image distortion that is caused by pronounced tractional membranes and perifoveal capillary leakage. Macular edema and its’ type, as well as epiretinal membrane bridging the fovea can precisely be defined and monitored via optical coherence tomography. This study included a total of 138 patients (188 eyes) with epiretinal membranes (95 on the right and 93 on the left eye). Macular edema was present in 144 eyes with, and 69 eyes without, epiretinal membranes. Data obtained for both eyes indicates that upon analysis number of patients in both examined groups (epiretinal membrane bridged and did not bridge the fovea) increases exponentially with increased visual acuity, and suggests that there is no considerable difference in distribution of visual acuity with regards to patients in both examined groups. This data also indicates that patients with the worst central macular thickness (>500μ) are represented only in the group of patients where epiretinal membrane had bridged the fovea, but that in both examined groups there is a difference in prevalence of patients with central macular thickness of 400 to 499μ (more common in the group that did, than in the group that did not, bridge the fovea). Hence, there exists a relationship between epiretinal membrane that bridges the fovea and central macular thickness. Aim of this study is to determine the extent in which epiretinal membrane bridging or not bridging the fovea influences visual acuity and to determine the relationship between them; central macular thickness; and visual acuity.","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79199940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}