Effect of Lentil and Buffalo Whey Protein Hydrolysates on Histopathology of Liver and Kidney in Diabetic Rats

This study was undertaken to evaluate the effects of protein hydrolysate prepared from lentil and buffalo whey proteins on body weight and histopathology of alloxan induced diabetic rats. Seventy two male albino rats were randomly divided into non-diabetic, diabetic untreated and diabetic treated with lentil or buffalo whey protein hydrolysates with or without yogurt. Experimental diabetes was induced by a single intraperitoneal (i.p) administration of alloxan (120 mg/kg body weight). Histopathological analyses were conducted on the liver and kidney tissues after 28 day of experiment. While the body weight was significantly decreased in the diabetic control group, there were no significant difference in diabetic treated group (P<0.05). Destruction of the liver architecture of the hepatocytes in the diabetic group showed the signs of steatosis, fibrosis and inflammation in the central vein and blood vessels. In the kidney, intestinal nephrites and mesangial proliferation and formation of cast material were observed. Yoghurt-containing lentil protein hydrolysate contained in yogurt appeared to have remarkable effect to prevent damage in liver and kidney histology caused by diabetes.