Journal of clinical & cellular immunology最新文献

{"title":"Investigating Molecular Connections of Non-alcoholic Fatty Liver Disease with Associated Pathological Conditions in West Virginia for Biomarker Analysis.","authors":"Dana L Sharma,&nbsp;Hari Vishal Lakhani,&nbsp;Rebecca L Klug,&nbsp;Brian Snoad,&nbsp;Rawan El-Hamdani,&nbsp;Joseph I Shapiro,&nbsp;Komal Sodhi","doi":"10.4172/2155-9899.1000523","DOIUrl":"https://doi.org/10.4172/2155-9899.1000523","url":null,"abstract":"<p><p>Non-alcoholic fatty liver disease (NAFLD) is a disease characterized by a steatosis of the liver that may progress to more serious pathological conditions including: nonalcoholic steatohepatitis (NASH), fibrosis, and cirrhosis. As the prevalence of NAFLD has increased worldwide in recent years, pathophysiology and risk factors associated with disease progression of NAFLD are at the focus of many studies. NAFLD is related to and shares common serum biomarkers with cardiovascular disease (CVD), type 2 diabetes mellitus (T2DM), obesity, and metabolic syndrome (MetS). West Virginia (WV) is a state with some of the highest rates of CVD, obesity and diabetes mellitus. As NAFLD is closely related to these diseases, it is of particular interest in WV. Currently there is no cost-effective, standardized method used clinically to detect NAFLD prior to the onset of reversible complications. At this time, the diagnosis of NAFLD is made with costly radiologic studies and invasive biopsy. These studies are only diagnostic once changes to hepatic tissue have occurred. The diagnosis of NAFLD by traditional methods may not allow for successful intervention and may not be readily available in areas with already sparse medical resources. In this literature review, we identify a list of biomarkers common among CVD, T2DM, obesity, MetS and NAFLD. From this research we propose the following biomarkers are good candidates for inclusion in a panel of biomarkers for the early detection of NAFLD: adiponectin, AST, ALT, apo-B, CK18, CPS1, CRP, FABP-1, ferritin, GGT, GRP78, HDL-C, IGF-1, IL-1β, 6, 8, 10, IRS-2PAI-1, leptin, lumican, MDA SREBP-1c and TNF-α. Creating and implementing a biomarker panel for the early detection and attenuation of NAFLD, prior to the onset of irreversible complication would provide maximum benefit and decrease the disease burden on the patients and healthcare system of WV.</p>","PeriodicalId":15473,"journal":{"name":"Journal of clinical & cellular immunology","volume":"8 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2155-9899.1000523","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35640537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Myeloid Cells in Immunity to Malaria: A Review","authors":"C. Uchechukwu, Chinedu Eleonu Priscella, Iwuala Egondu, O. Florence","doi":"10.4172/2155-9899.1000519","DOIUrl":"https://doi.org/10.4172/2155-9899.1000519","url":null,"abstract":"Malaria has continued to be a major cause of morbidity and mortality in the Tropical World. Research on its complex immunology has focused more on the host adaptive immunity to the plasmodium parasite. The role of innate immune mechanisms involving myeloid cells has not been given adequate attention. This review highlights the key role of myeloid cells in immunity to malaria through such mechanisms as parasite sensing and elimination, pro inflammatory activities and activation of other immune components.","PeriodicalId":15473,"journal":{"name":"Journal of clinical & cellular immunology","volume":"41 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2017-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76360194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Receptors for Interleukins and Tumor Necrosis Factor are Important in Assessing their Roles in CNS Disorders","authors":"P. Szot","doi":"10.4172/2155-9899.1000520","DOIUrl":"https://doi.org/10.4172/2155-9899.1000520","url":null,"abstract":"The immune-to-brain communication is still in its infancy but there is a great deal of data to suggest its importance in several central nervous system (CNS) disorders. There are three cytokines, interleukin-1 (IL-1), IL-6 and tumor necrosis factor alpha (TNFα), which have emerged to have a major role in the CNS and in different CNS disorders. The majority of the published work to date has been on examining changes in the levels of these proteins in the CNS with inflammation; but recent work from our laboratory has shown the receptors for these cytokines may also be an important factor in neuroinflammation mediated CNS disorders, because these receptors are solely localized to neurons and are modified when their ligands levels are elevated. For neuroinflammation and the increase in cytokine levels (either by glia or neurons) to influence neurons and consequently affect the development of CNS disorders, the location of these cytokines receptors on neuronal populations may be the key.","PeriodicalId":15473,"journal":{"name":"Journal of clinical & cellular immunology","volume":"99 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2017-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79305012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunoinformatics-Peptide Driven Vaccine and In silico Modeling for Duvenhage Rabies Virus Glycoprotein G","authors":"S. O. Albagi, O. H. Ahmed, Manal A Gumaa, K. Abdelrahman, Ahmed Hamdi Abu-haraz, Mohammed A Hassan","doi":"10.4172/2155-9899.1000517","DOIUrl":"https://doi.org/10.4172/2155-9899.1000517","url":null,"abstract":"Background: Duvenahge rabies virus belongs to Lyssavirus genus family Rhabdoviridae causing fatal infection with no effective treatment available and no licensed DNA or peptide vaccine up to date. The aim of present study was to predict peptide vaccine for Duvenhage rabies virus. \u0000Methods and materials: The sequences of Duvenahge virus was optioned from NCBI, and then it was subjected to many B cell and T cell tests from IEDB to realize the most promising peptides that could act as driven-peptide vaccine. Population coverage analysis was performed for selected peptides using IEDB and finally homology modeling and molecular docking studies were done to visualize the interaction with MHC1 molecules. \u0000Result and conclusion: Among the tested peptides for T cell-test, this study projected an interesting epitope of T cell (YFLIGVSAV) that exhibit all-consuming of binding affinity as strong indicator to MHC-One and MHC-two alleles together, besides the binding to eighteen alleles through the population coverage 99.36% in the world. These results were further supported by molecular docking studies that show excellent interaction with MHC homo spins molecule with the lowest binding energy among tested peptides. \u0000Only three B cell epitopes (AHYK, YTIPDKL and SLHNPYPDSH) were found to overlap all performed B cell tests by being linear, on the surface of glycoprotein G and being antigenic.","PeriodicalId":15473,"journal":{"name":"Journal of clinical & cellular immunology","volume":"1 1","pages":"1-14"},"PeriodicalIF":0.0,"publicationDate":"2017-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84445332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between Interleukin-18-137G/C Gene Polymorphisms and Tuberculosis Risk in Chinese Population: A Meta-Analysis","authors":"Shen Longqiang, Zhang Liang, Cuiping Xu, Jiaru Yang, Han Xinlin, Zhao Hua, Aihua Liu, Fukai Bao","doi":"10.4172/2155-9899.1000514","DOIUrl":"https://doi.org/10.4172/2155-9899.1000514","url":null,"abstract":"This study was to investigate the relationship between IL-18-137G/C polymorphism and TB risk by meta-analysis. The literatures about the IL-18-137G/C polymorphism and risk of tuberculosis were selected from four English databases and four Chinese databases. Data were extracted from the studies by two independent reviewers. Statistical analysis was executed using Revman 5.3 and Stata 11.0 software. A total of 5 studies with 558 TB patient and 720 controls were included in this meta-analysis, The results showed that-137G/C polymorphisms in the IL-18 gene were associated with TB risk in china when taking comparisons of the G allele vs. C allele (OR=1.49, 95% CI=1.21-1.84, P=0.0002), GG vs. GC+X.06, P=0.0003). It was also significant in the subgroup analysis of Chinese adults (G allele vs. C allele: OR=1.32, 95%=CI 1.03-1.70, P=0.003; GG vs. GC+CC: OR=1.39, 95% CI=1.01-1.91, P=0.04) and Chinese children (G allele vs. C allele: OR=1.91, 95% CI=1.31-2.78, P=0.0008; GG vs. GC+CC: OR=2.02, 95% CI=1.33-3.07, P=0.0010). This study provides the evidence that the allele G of IL-18-137G/C polymorphism was closely associated with TB risk in China.","PeriodicalId":15473,"journal":{"name":"Journal of clinical & cellular immunology","volume":"47 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2017-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85460974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Semaphorin 5A, Anti-Nucleosome Antibodies and Ferritin: Disease Activity Markers in Patients with Systemic Lupus Erythematosus","authors":"Amany M. Elsaeed, Sabah E. Abd-Elraheem, Amal H. Ibrahiem, E. Mohamed, O. Ali, H. Mansour","doi":"10.4172/2155-9899.1000516","DOIUrl":"https://doi.org/10.4172/2155-9899.1000516","url":null,"abstract":"Background: Systemic Lupus Erythematosus (SLE) is a multi-factorial, chronic autoimmune disorder characterized by dysfunction of T and B lymphocytes. Although the prognosis of SLE has improved in the past few decades, the absence of biomarkers for residual activity in various organs and the early detection of disease flares hamper further management. Conventional serologic markers of SLE, such as anti-dsDNA and complement levels, are not ideal, as they are not sufficiently sensitive and specific for the diagnosis of the disease and monitoring of disease activity. Thus, novel biomarkers for SLE activity have to be developed. Semaphorin 5A (Sema 5A), antinuclosome antibody (Anu A) and ferritin may fall into this category of novel bio markers.Objective: To evaluate the role of serum Sema 5A, Anu A and ferritin in detection of SLE activity.Methods: The present study included 40 patients with SLE divided according to Systemic Lupus Erythematous Disease Activity Index (SLEDAI) into two groups: 20 active SLE and 20 inactive SLE patients. They were compared with 20, sex and age matched healthy individuals for control. Levels of Sema 5A, Anu A were measured by Enzyme- Linked Immunosorbent Assay (ELISA) and serum ferritin levels were measured by Electrochemiluminescence immunoassay (ECLIA).Results: There was highly significant increase in Sema 5A, Anu A and ferritin levels inactive when compared within active SLE patients and control subjects (P=0.000 and P=0.000 and P=0.000 respectively). There was a significant increase in ferritin level in inactive SLE patients when compared with control subjects (P=0.045) with no significant difference regarding Sema 5A, Anu A (P3=0.089 and 0.225 respectively). There were positive correlations between Sema 5A, Anu A, ferritin and each of SLEDAI, and CRP. Positive correlation also found between Sema 5A and each of Anu A and ferritin and between AnuA and ferritin. Negative correlation was found between Sema 5A and C3 and between Anu A, ferritin and C4. Significant relation was found between Sema 5A, Anu A, ferritin and each of ANA and anti-dsDNA in active SLE patients. Area under the curve (AUC) for ferritin, Sema 5A and Anu A were (0.861, 1.0 and 1.0 respectively).Conclusion: Our study showed that the serum proteins semaphorin 5A, antinuclosome antibodies and ferritin may be useful markers in monitoring disease activity in patients with SLE. Regarding area under the curve (AUC), these serum protein markers result in even better sensitivity and specificity profiles in picking up early relapse of SLE.","PeriodicalId":15473,"journal":{"name":"Journal of clinical & cellular immunology","volume":"51 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2017-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88452632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kflc Index Evaluation to Assess Immunoglobulin Intrathecal Synthesis","authors":"M. Pieri, R. Zenobi, M. Dessi","doi":"10.4172/2155-9899.1000515","DOIUrl":"https://doi.org/10.4172/2155-9899.1000515","url":null,"abstract":"Multiple sclerosis (MS), where intrathecal synthesis is present, is one of the most common neurological diseases of the young adults in CNS, and it often causes deficits since its early stage. Clinically isolated syndrome (CIS) is a central nervous system demyelinating event isolated in time and it is compatible with the possible future development of multiple sclerosis (MS). Early risk stratification for conversion to MS helps with treatment decisions [3]. Currently it is possible to identify the presence of antibodies by the detection of oligoclonal immunoglobulin bands (OCBs) in cerebrospinal fluid (CSF) [4,5]. It is necessary to differentiate the origin of immunoglobulin (Ig) in the CSF before intrathecal immunoglobulin synthesis can be diagnosed [6]. Immunoglobulin light chains that are circulating in serum in a free state are called Free Light Chains (FLCs). These can be both kappa (kFLCs) and lambda type (λFLCs). We examined 80 CSF/serum pairs from patients of the neurological clinic who underwent analysis of OCBs during their diagnostic workup. We analysed the correlation between OCBs test and the FLCs nephelometric assay [1] and we assessed the diagnostic accuracy of a nephelometric assay for k free light chain determination in cerebrospinal fluid and serum. The correlation between the two methods was very good, but the method to determine OCBs is timeconsuming, not quantitative, and also relatively insensitive and operator depending. [1,2,7-9]. On the contrary, the nephelometric determination of FLCs in serum and CSF is a quantitative method and it might be a sensitive alternative to the above-mentioned approach.","PeriodicalId":15473,"journal":{"name":"Journal of clinical & cellular immunology","volume":"68 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2017-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90767605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conserved HIV Epitopes for an Effective HIV Vaccine.","authors":"Bikash Sahay,&nbsp;Cuong Q Nguyen,&nbsp;Janet K Yamamoto","doi":"10.4172/2155-9899.1000518","DOIUrl":"https://doi.org/10.4172/2155-9899.1000518","url":null,"abstract":"<p><p>Despite major advances in antiretroviral therapy against HIV-1, an effective HIV vaccine is urgently required to reduce the number of new cases of HIV infections in the world. Vaccines are the ultimate tool in the medical arsenal to control and prevent the spread of infectious diseases such as HIV/AIDS. Several failed phase-IIb to -III clinical vaccine trials against HIV-1 in the past generated a plethora of information that could be used for better designing of an effective HIV vaccine in the future. Most of the tested vaccine candidates produced strong humoral responses against the HIV proteins; however, failed to protect due to: 1) the low levels and the narrow breadth of the HIV-1 neutralizing antibodies and the HIV-specific antibody-dependent Fc-mediated effector activities, 2) the low levels and the poor quality of the anti-HIV T-cell responses, and 3) the excessive responses to immunodominant non-protective HIV epitopes, which in some cases blocked the protective immunity and/or enhanced HIV infection. The B-cell epitopes on HIV for producing broadly neutralizing antibodies (bNAbs) against HIV have been extensively characterized, and the next step is to develop bNAb epitope immunogen for HIV vaccine. The bNAb epitopes are often conformational epitopes and therefore more difficult to construct as vaccine immunogen and likely to include immunodominant non-protective HIV epitopes. In comparison, T-cell epitopes are short linear peptides which are easier to construct into vaccine immunogen free of immunodominant non-protective epitopes. However, its difficulty lies in identifying the T-cell epitopes conserved among HIV subtypes and induce long-lasting, potent polyfunctional T-cell and cytotoxic T lymphocyte (CTL) activities against HIV. In addition, these protective T-cell epitopes must be recognized by the HLA prevalent in the country(s) targeted for the vaccine trial. In conclusion, extending from the findings from previous vaccine trials, future vaccines should combine both T- and B-cell epitopes as vaccine immunogen to induce multitude of broad and potent immune effector activities required for sterilizing protection against global HIV subtypes.</p>","PeriodicalId":15473,"journal":{"name":"Journal of clinical & cellular immunology","volume":"8 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2155-9899.1000518","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35239068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Fas and RANKL Signaling in Peripheral Immune Tolerance","authors":"T. Izawa, R. Arakaki, N. Ishimaru","doi":"10.4172/2155-9899.1000512","DOIUrl":"https://doi.org/10.4172/2155-9899.1000512","url":null,"abstract":"The death receptor, Fas, has been well-characterized and is a critical factor in apoptosis in immune cells. Fas also has an important role in maintaining immune tolerance as demonstrated in the autoimmune-prone MRL/lpr mouse strain which carries a defect in Fas-mediated apoptosis of T cells. However, the role of Fas-independent apoptosis remains to be characterized in autoimmune diseases. In dendritic cells (DCs), binding of receptor activator of nuclear factor-κB ligand (RANKL) to RANK perpetuates the survival of mature DCs. However, cross-talk between the RANK/RANKL pathway and Fas-mediated signaling during the function or activation of DCs has not been well-studied. This short communication review describes a mechanism involving interactions between activated DCs and T cells in the autoimmune response of MRL/lpr mice and a novel Fas-independent apoptosis pathway in T cells that maintains peripheral tolerance, and controls autoimmunity in MRL/lpr mice.","PeriodicalId":15473,"journal":{"name":"Journal of clinical & cellular immunology","volume":"36 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2017-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87467371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Association between Anti-Nuclear Antibodies and Obesity is Likely Mediated by Abdominal Adiposity and Systemic Inflammation","authors":"I. Blanco, M. Labitigan, M. Abramowitz","doi":"10.4172/2155-9899.1000513","DOIUrl":"https://doi.org/10.4172/2155-9899.1000513","url":null,"abstract":"Background: Obesity and abdominal adiposity have been associated with inflammation as have the presence of anti-nuclear antibodies (ANAs). It was recently reported that there is a decreased likelihood of ANAs in the obese general population. To examine this relationship we used data from adult participants in the National Health and Nutrition and Examination Survey 1999-2004. \u0000Methods: Participants were excluded if they reported a history of arthritis other than osteoarthritis, thyroid or liver disease, or steroid use so as to rule out a history of possible prior autoimmune disease. We strictly defined a positive ANA as a titer ≥ 1:160. Overweight and obesity were classified using traditional BMI criteria. High and low C-reactive protein (CRP) were defined using the 75th percentile cutpoint as ≥0.42 and <0.42 mg/dL, respectively. Dual-energy X-ray absorptiometry (DEXA) was used to determine body composition. Logistic regression models were created to examine associations with ANA status. \u0000Results: 2552 participants were included in our analyses. Obese participants were older (p<0.001), more likely to be men (p=0.004) and to have comorbidities, and had higher levels of CRP (<0.001). After multivariable adjustment, obesity was associated with a decreased odds of having ANAs (OR 0.78, 95%CI 0.62-0.99). However when adding log-transformed CRP into our model, this association was no longer significant (OR 0.85, 95%CI 0.62-1.15), and there was evidence of effect modification by CRP (p=0.12). Among participants with low CRP, obesity was again associated with a reduced likelihood of ANA positivity (OR 0.69, 95%CI 0.48-0.99), but a trend was seen in the opposite direction in those with high CRP (OR 1.77, 95%CI 0.81-3.88). When looking at the 1143 obese and overweight participants with low CRP, ANA positivity was associated with a higher prevalence of cardiovascular disease (p=0.02) and higher % total body fat (p=0.007), trunk fat (p=0.02), and non-trunk fat (p=0.004). This association, however, was not found in the high CRP group. \u0000Conclusion: In the general population the association of obesity with ANA is modified by the presence of systemic inflammation as measured by CRP, where the inverse association previously found is eliminated when controlling for CRP. This inverse relationship remains among obese participants with low CRP, when these obese and overweight participants are ANA positive; it is associated with greater total body and trunk fat. It is possible that body composition is driving autoimmunity in the general population even in the absence of systemic inflammation.","PeriodicalId":15473,"journal":{"name":"Journal of clinical & cellular immunology","volume":"53 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2017-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86376027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}