Immunoinformatics-Peptide Driven Vaccine and In silico Modeling for Duvenhage Rabies Virus Glycoprotein G

Abstract

Background: Duvenahge rabies virus belongs to Lyssavirus genus family Rhabdoviridae causing fatal infection with no effective treatment available and no licensed DNA or peptide vaccine up to date. The aim of present study was to predict peptide vaccine for Duvenhage rabies virus. Methods and materials: The sequences of Duvenahge virus was optioned from NCBI, and then it was subjected to many B cell and T cell tests from IEDB to realize the most promising peptides that could act as driven-peptide vaccine. Population coverage analysis was performed for selected peptides using IEDB and finally homology modeling and molecular docking studies were done to visualize the interaction with MHC1 molecules. Result and conclusion: Among the tested peptides for T cell-test, this study projected an interesting epitope of T cell (YFLIGVSAV) that exhibit all-consuming of binding affinity as strong indicator to MHC-One and MHC-two alleles together, besides the binding to eighteen alleles through the population coverage 99.36% in the world. These results were further supported by molecular docking studies that show excellent interaction with MHC homo spins molecule with the lowest binding energy among tested peptides. Only three B cell epitopes (AHYK, YTIPDKL and SLHNPYPDSH) were found to overlap all performed B cell tests by being linear, on the surface of glycoprotein G and being antigenic.