Journal of clinical & cellular immunology最新文献_第4页

Doughnut Cells or Ring Neutrophil by Immunofluorescence 免疫荧光法测定甜甜圈细胞或环状中性粒细胞

Journal of clinical & cellular immunology Pub Date : 2020-01-01 DOI: 10.35248/2155-9899.20.11.597

S. B. Nascimento, M. A. Pereira, H. Kang

引用次数: 1

The JAK-STAT Signaling Pathway: A Novel Therapeutic Target for Unexplained Recurrent Implantation Failures JAK-STAT信号通路:不明原因复发性植入失败的新治疗靶点

Journal of clinical & cellular immunology Pub Date : 2020-01-01 DOI: 10.35248/2155-9899.20.11.587

Alphonsus Ogbonna Ogbuabor, P. Achukwu, D. Ogbuabor, Sa Ufelle, Rapheal Chinweike Okolo

{"title":"The JAK-STAT Signaling Pathway: A Novel Therapeutic Target for Unexplained Recurrent Implantation Failures","authors":"Alphonsus Ogbonna Ogbuabor, P. Achukwu, D. Ogbuabor, Sa Ufelle, Rapheal Chinweike Okolo","doi":"10.35248/2155-9899.20.11.587","DOIUrl":"https://doi.org/10.35248/2155-9899.20.11.587","url":null,"abstract":"Unexplained recurrent implantation failure has become a public health problem especially in the context of a high cost, demand for assisted reproductive therapy and multiple therapy failures. It is attributed to the failure of immunological tolerance between the transferred embryo and the endometrium during assisted reproductive therapy cycles. Cytokines are surrogate mediators of immunological tolerance mechanisms. Since the synergistic interaction between individual cytokines is dynamic, perturbations in the cytokine crosstalk during embryo implantation is considered a major etiology for unexplained recurrent implantation failures. Genome Wide Association Studies suggests that most cytokines initiate their actions through receptor interactions that activate the JAK-STAT signaling pathways. Aberrations in the JAK-STAT signaling pathways have as well been shown to cause perturbations in cytokine crosstalk that result in diseases. We therefore propose the JAK-STAT signaling pathway a potential therapeutic target for unexplained recurrent implantation failures. Currently, many studies have recorded potential therapies in IL-6/JAK/STAT 3 pathway to treat many diseases but limited attention has been paid to unexplained recurrent implantation failures.","PeriodicalId":15473,"journal":{"name":"Journal of clinical & cellular immunology","volume":"29 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84710496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Prenatal Corticosterone Exposure Alters Glucocorticoid Metabolic Enzyme Eene mRNA Associated with Increased Aggressive Behaviors and Tonic Immobility in Chicken 产前皮质酮暴露改变鸡糖皮质激素代谢酶Eene mRNA与攻击行为和补益不动行为增加相关

Journal of clinical & cellular immunology Pub Date : 2020-01-01 DOI: 10.35248/2155-9899.20.11.604

Abdelkareem A. Ahmed, M. Essa, A. Mollica, A. Stefanucci, G. Zengin, Hussain Ahmed, Ayman Sati Sati Mohamed

{"title":"Prenatal Corticosterone Exposure Alters Glucocorticoid Metabolic Enzyme Eene mRNA Associated with Increased Aggressive Behaviors and Tonic Immobility in Chicken","authors":"Abdelkareem A. Ahmed, M. Essa, A. Mollica, A. Stefanucci, G. Zengin, Hussain Ahmed, Ayman Sati Sati Mohamed","doi":"10.35248/2155-9899.20.11.604","DOIUrl":"https://doi.org/10.35248/2155-9899.20.11.604","url":null,"abstract":"Exposure to excess Glucocorticoids (GCs) during embryonic development influences offspring physiology and behaviors and induces change in Hypothalamic-Pituitary-Adrenal (HPA) axis genes expression and serotonergic system in mammals. Whether prenatal corticosterone (CORT) exposure induces similar effects in avian species remains unclear. In the present study, we injected low (0.2 μg) and high (1 μg) doses of CORT in ovo before incubation and detected changes in aggressive behavior, Tonic Immobility (TI), HPA axis and 5-hydroxytryptamine (serotonin) (5-HT) system gene expression on post hatch chickens of different ages. High dose of CORT significantly (P<0.05) suppressed growth rate, increased the frequency of aggressive behaviors, which was associated with elevated plasma CORT concentration. Likewise, in ovo injection of CORT significantly (P<0.05) increased Tonic Immobility (TI) duration both in chickens from low and high doses of CORT treatments compared to control. In addition, administration of CORT significantly (P<0.05) up-regulated mRNA expression of 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) whereas it down-regulated 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2) and mineralocorticoid receptor (MR) mRNA expression in the hypothalamus. No significant differences were seen in Glucocorticoid Receptor (GR) and 20-hydroxysteroid dehydrogenase (20-HSD) mRNA levels upon CORT treatment. Moreover, CORT exposure significantly (P<0.05) increased hypothalamic 5-hydroxytryptamine (serotonin) receptor 1A (5-HTR1A) mRNA expression, but not 5-HT receptor 1B (5-HTR1B). In ovo administration of CORT may programs the aggressive behaviors in the chicken through alterations of HPA axis and 5-HT system.","PeriodicalId":15473,"journal":{"name":"Journal of clinical & cellular immunology","volume":"74 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86655577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Long Non-Coding RNA Hotairm1 Promotes S100A9 Support of MDSC Expansion during Sepsis. 长链非编码RNA Hotairm1促进S100A9在脓毒症期间支持MDSC扩增

Journal of clinical & cellular immunology Pub Date : 2020-01-01 Epub Date: 2020-09-22

Tuqa Alkhateeb, Isatou Bah, Ajinkya Kumbhare, Dima Youssef, Zhi Q Yao, Charles E McCall, Mohamed El Gazzar

{"title":"Long Non-Coding RNA Hotairm1 Promotes S100A9 Support of MDSC Expansion during Sepsis.","authors":"Tuqa Alkhateeb, Isatou Bah, Ajinkya Kumbhare, Dima Youssef, Zhi Q Yao, Charles E McCall, Mohamed El Gazzar","doi":"","DOIUrl":"","url":null,"abstract":"Myeloid-derived suppressor cells (MDSCs) expand during mouse and human sepsis, but the mechanism responsible for this is unclear. We previously reported that nuclear transport of S100A9 protein programs Gr1+CD11b+ myeloid precursors into MDSCs in septic mice. Here, we show that long non-coding RNA Hotairm1 converts MDSCs from an activator to a repressor state. Mechanistically, increased Hotairm1 expression in MDSCs in mice converted S100A9 from a secreted proinflammatory mediator to an immune repressor by binding to and shuttling it from cytosol to nucleus during late sepsis. High Hotairm1 levels were detected in exosomes shed from MDSCs from late septic mice. These exosomes inhibited lipopolysaccharide-stimulated secretion of S100A9 from early sepsis Gr1+CD11b+ cells. Importantly, Hotairm1 knockdown in late sepsis Gr1+CD11b+ MDSCs prevented S100A9 cytosol to nuclear transfer and decreased repression of proimmune T cells. Notably, ectopic expression of Hotairm1 in early sepsis Gr1+CD11b+ cells shuttled S100A9 to the nucleus and promoted the MDSC repressor phenotype. In support of translating the mechanistic concept to human sepsis, we found that Hotairm1 binds S100A9 protein in CD33+CD11b+HLA-DR- MDSCs during established sepsis. Together, these data support that Hotairm1 is a plausible molecular target for treating late sepsis immune suppression in humans and its immune repressor mechanism may be cell autonomous.","PeriodicalId":15473,"journal":{"name":"Journal of clinical & cellular immunology","volume":"11 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7744002/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38387154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Type I and II Interferon are Associated with High Expression of the Hippo Pathway Family Members I型和II型干扰素与Hippo通路家族成员的高表达相关

Journal of clinical & cellular immunology Pub Date : 2020-01-01 DOI: 10.35248/2155-9899.20.11.585

Bianca Sciescia, R. Tognon, N. Nunes, T. Malta, F. Frantz, F. Castro, M. Cacemiro

引用次数: 0

Activation of the classical complement pathway and auto-immune diseases 经典补体途径的激活与自身免疫性疾病

Journal of clinical & cellular immunology Pub Date : 2019-01-28 DOI: 10.4172/2155-9899-C4-058

pJamal Almitairip

引用次数: 1

Gene Expression Analysis on Neutrophil-Dendritic Hybrid Cells in Synovial Fluid from Rheumatoid Arthritis (RA) Patient 类风湿性关节炎(RA)患者滑液中性粒细胞-树突状杂交细胞基因表达分析

Journal of clinical & cellular immunology Pub Date : 2019-01-01 DOI: 10.4172/2155-9899.1000575

Yun Liu, T. Ota, Xiaohuan Chen, Hallie Dolin, K. Pan, A. Takashima, Atsushi Hinohara, Z. Pan

引用次数: 0

Atherosclerosis and cardiovascular disease in patients with rheumatic disease, with focus on SLE: risk factors and underlying mechanisms 风湿性疾病患者的动脉粥样硬化和心血管疾病，重点是SLE:危险因素和潜在机制

Journal of clinical & cellular immunology Pub Date : 2018-12-17 DOI: 10.4172/2155-9899-C6-062

pJohan Frostegardp

引用次数: 0

Achieving Sustained Viral Remission in Patients with Chronic HCV Infection and Cryoglobulinemic Vasculitis Does Not Always Correlate with Normalization of the Serologic Markers. 慢性丙型肝炎病毒感染和冷球蛋白性血管炎患者的持续病毒缓解并不总是与血清学标志物的正常化相关。

Journal of clinical & cellular immunology Pub Date : 2018-10-03 DOI: 10.4172/2155-9899.1000562

Aaron Stubbs, Corinne Kowal, Ali Askari, Donald D Anthony, Maya Mattar

{"title":"Achieving Sustained Viral Remission in Patients with Chronic HCV Infection and Cryoglobulinemic Vasculitis Does Not Always Correlate with Normalization of the Serologic Markers.","authors":"Aaron Stubbs, Corinne Kowal, Ali Askari, Donald D Anthony, Maya Mattar","doi":"10.4172/2155-9899.1000562","DOIUrl":"https://doi.org/10.4172/2155-9899.1000562","url":null,"abstract":"Objective: We aim to describe the persistence of symptoms associated with HCV-associated cryoglobulinemic vasculitis following achievement of (SVR) with IFN- free direct acting antiviral (DAA) therapy. In particular, we describe the persistence of C4 hypocomplementemia and positive Rheumatoid Factor (RF).Methods: We analyzed a case series of four patients enrolled from the Cleveland VA and known to have chronic HCV infection complicated by mixed cryoglobulinemia. The study included patients treated with interferon (IFN) based treatment and IFN free direct acting antiviral (DAA) therapy.Results: Of the four patients, patients 1 and 2 experienced decline of RF without resolution following DAA therapy. Patient 1 continues to have evidence of disease following treatment. Patient 3 did not have resolution of RF during IFN-based treatment and experienced stabilization of kidney function while on treatment. Patient 4, previously a non-responder to IFN based treatment, experienced significant decline in RF titers along with resolution of cryoglobulin-associated rash with DAA therapy. C4 remained low following treatment in patients 1 and 3. Of the four patients, only patient 1 had prolonged persistence of cryoglobulinemia, measured at 3%, 17 months following achievement of SVR.Conclusions: We highlight the complexity of the viral-mediated immunologic mechanism that causes cryoglobulinemic vasculitis. Our cases also emphasize the need to consider cryoglobulinemic vasculitis as part of the differential diagnosis even with treated HCV infection. Recognizing these findings are important in our understanding of the pathophysiology of the disease and management in the era of IFN-free DAA therapy.","PeriodicalId":15473,"journal":{"name":"Journal of clinical & cellular immunology","volume":"9 5","pages":"562"},"PeriodicalIF":0.0,"publicationDate":"2018-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2155-9899.1000562","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37304164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Transcriptomic Profiling of the Immune Response to Crowding Stress in Juvenile Turbot ( Scophthalmus maximus ) 大菱鲆(Scophthalmus maximus)幼鱼对拥挤胁迫免疫反应的转录组学分析

Journal of clinical & cellular immunology Pub Date : 2018-09-21 DOI: 10.4172/2155-9899.1000559

H. Huo, Xiao‐Qiang Gao, Fan Fei, Fei Qin, Bin Huang, Baoliang Liu

{"title":"Transcriptomic Profiling of the Immune Response to Crowding Stress in Juvenile Turbot ( Scophthalmus maximus )","authors":"H. Huo, Xiao‐Qiang Gao, Fan Fei, Fei Qin, Bin Huang, Baoliang Liu","doi":"10.4172/2155-9899.1000559","DOIUrl":"https://doi.org/10.4172/2155-9899.1000559","url":null,"abstract":"In this study, juvenile turbot Scophthalmus maximus were vaccinated with attenuated Edwardsiella tarda and reared in two different densities, low density (LD, 5.25 ± 0.02 kg/m2) as control groups and high density (HD, 20.53 ± 0.05 kg/m2) as experimental groups, and only density as variable was considered. Five weeks after vaccination, the transcriptomes of spleen and head kidney from the turbot in the two density groups were analyzed with RNA-Seq technology. A total of 447 million reads were assembled into 41,136 genes with an average length of 1274 bp and N50 size of 2295 bp. A comparison of gene expression between HD and LD revealed 1155 differentially expressed genes (DEGs), including 246 significantly upregulated unigenes (fold-change>2) and 909 significantly downregulated unigenes (fold-change>2). Enrichment and pathway analysis of the immune-related DEGs showed the centrality of the Toll-like receptor signaling pathway, cytosolic DNA-sensing pathway and platelet activation in the host immune responses. The overexpressed inflammatory cytokines and downregulated signal-regulated cytokines are involved in these pathways. We inferred that overexpressed inflammatory cytokines indicate that cells suffer damage and downregulated signal pathway-related cytokines indicate the immune response is restrained in turbot under crowding stress. Our results increase understanding of the effect of crowding stress on immunosuppression and provide valuable information on healthful aquaculture strategies in juvenile turbot.","PeriodicalId":15473,"journal":{"name":"Journal of clinical & cellular immunology","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75072769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1