Journal of clinical & cellular immunology最新文献

{"title":"Association of CD4+ CD25+ FOXP3+ regulatory T-cells and human papilloma virus infection in Egyptian Women with breast cancer","authors":"A. Tawfeik, A. Mora, A. Osman, M. Moneer, N. El-sheikh, M. Elrefaei","doi":"10.21203/rs.2.17376/v2","DOIUrl":"https://doi.org/10.21203/rs.2.17376/v2","url":null,"abstract":"\u0000 Several subsets of regulatory CD4+ T cells (CD4+ Tregs) have been described in peripheral blood and tumor microenvironment and blood of breast cancer (BC) patients and may play a key role in the progression of BC. High-risk human papilloma virus (HPV) have a causal role in a significant proportion of cervical, and head, and neck tumors and may play an important role in evoking neoplasia in BC. In this study we assessed the prevalence of CD4+Tregs (CD4+CD25+ FOXP3+ cells) and CD3+ CD8+ T cells by flow cytometry in peripheral blood from a total of 55 Egyptian women, including 20 treatment-naïve BC, 15 with breast benign lesions (BBL) and 20 healthy volunteers (HV). High-risk HPV genotype type 16, 18, and 31 was investigated in breast tissue from all BC and BBL patients using Real-Time PCR. HPV was detected in 4 BC, but in none of BBL patients. The frequency of CD4+ Tregs was significantly higher in BC compared to BBL and HV, (p < 0.001). In addition, we observed a significantly higher frequency of CD3+ CD8+ T cells in peripheral blood of patients with late stage III compared to early stage I and II BC (p = 0.011). However, there was no significant association between the ratio of CD8+ T cell to CD4+ Tregs frequencies and the expression of Estrogen Receptor (ER), Progesterone Receptor (PR), and Human Epidermal Growth Factor Receptor 2 (HER2). In conclusion, CD4+ Tregs may contribute to progression BC in Egyptian women with HPV infection. The potential role CD4+ Tregs as a prognostic or predictive parameter should be analyzed in a larger longitudinal study with sufficient follow-up time.","PeriodicalId":15473,"journal":{"name":"Journal of clinical & cellular immunology","volume":"43 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2020-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81528928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fracture of a Dorsal Vertebral Body as the First Manifestation of Pott's Disease: Presentation of Clinical Case","authors":"Daniela Ledezma Gonz ¡lez, Carolina Armeaga Azo ±os, S. RicardoCoronado, oval","doi":"10.35248/2155-9899.20.11:594","DOIUrl":"https://doi.org/10.35248/2155-9899.20.11:594","url":null,"abstract":"Introduction: Infection with tuberculosis (Mycobacterium tuberculosis) has become a disease with high incidence and prevalence, due to its dissemination pathway and its pathophysiological mechanisms, which make it an entity with variable clinical presentation. One of these presentations is tuberculous osteomyelitis or PottDisease. This syndrome occurs when, by hematogenous dissemination, the mycobacterium spreads to the spinal column, most commonly in the dorsal region, causing pain, paresis, abscesses, and deformity and, in advanced cases, paraplegia due to neurological damage secondary to the infection. Clinical case: In January 2018 of a male patient of 34 years old, resident of the San Quintin community, Baja California , Mexico with type 2 diabetes mellitus, Obesity grade III, and hepatic failure as comorbidities, who debuted with loss of mobility of lower extremities, an open magnetic resonance of the thoracolumbar spine was performed, finding T5 body collapse plus medullary infectious process, with subsequent diagnosis bytransquirurgical biopsy of Pott Disease , treated in the ISSSTECALI clinic, Ensenada,Baja California , Mexico during a hospitalization period of 31 days. Conclusion: Tuberculosis has an insidious evolution, especially when it is one of its extrapulmonary forms. Pott's disease is one of its many manifestations, which is important to detect in the early stages to prevent complications that compromise the patient's functionality. The treatment must be individualized according to the comorbidities of each patient.","PeriodicalId":15473,"journal":{"name":"Journal of clinical & cellular immunology","volume":"4 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82929951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association Study of PTPN22 (rs2476601) and PADI4 (rs2240340) Polymorphisms with Rheumatoid Arthritis in Algerian Population","authors":"I. Allam, M. Gharnaout, S. Louahchi, N. Raaf, Nawel Kheldoun, A. Ladjouze, R. Djidjik","doi":"10.35248/2155-9899.20.11.586","DOIUrl":"https://doi.org/10.35248/2155-9899.20.11.586","url":null,"abstract":"An association between protein tyrosine phosphatase 22 (PTPN22) and Peptidylarginine deiminase 4 (PADI4) genes with rheumatoid arthritis (RA) has been demonstrated in several populations. The present study investigated whether PTPN22 and PADI4 genes polymorphisms were involved in the genetic predisposition to RA in the Algerian patients. Materials and methods: The PADI4_94 (rs2240340) and the PTPN22 (rs2476601) Single Nucleotide Polymorphisms (SNPs) were genotyped in 300 RA patients and 306 healthy controls by real time polymerase chain reaction method (TaqMan Assays). The relationships between Anti-Citrullinated Peptide Antibody (ACPA) positivity, Rheumatoid Factor (RF) positivity and genotypes were statistically analyzed. Results: There was no significant association between the PTPN22, PADI4 SNP and RA susceptibility in our population (p>0.05). No association with ACPA profile with either PTPN22 or PADI4 was detected (p>0.05). However, our results showed a strong association of PTPN22 minor T allele with RF positive disease (OR=8.53 (95% CI 1.34-354.9), p=0.013); also, a significant association was shown between CT genotype of PTPN22 SNP and RF positive RA (OR=8.01 (95% CI 1.22-336.5), p=0.018). Conclusion: Our findings indicated that PTPN22 and PADI4 polymorphisms were unlikely to play an important role in the susceptibility to RA in Algerian population but PTPN22 polymorphism T allele may predispose individuals to RF positive RA.","PeriodicalId":15473,"journal":{"name":"Journal of clinical & cellular immunology","volume":"16 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89000418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The relative abundance of monocyte subsets determines susceptibility to perinatal hepatic inflammation.","authors":"Sarah Mohamedaly, A. Alkhani, A. Nijagal","doi":"10.35248/2155-9899.20.11.602","DOIUrl":"https://doi.org/10.35248/2155-9899.20.11.602","url":null,"abstract":"The devastating consequences of perinatal liver inflammation contribute to a pressing need to develop therapeutics for the diseases that underly this condition. Biliary atresia (BA) is a perinatal inflammatory disease of the liver that results in obliterative cholangiopathy and rapidly progresses to liver failure, requiring transplantation. The ability to develop targeted therapies requires an understanding of the immune mechanisms that mitigate perinatal liver inflammation. This article reviews our recent findings demonstrating that in a murine model of perinatal hepatic inflammation, Ly6cLo non-classical monocytes express a pro-reparative transcriptomic profile and that the relative abundance of Ly6cLo monocytes promotes resolution of perinatal liver inflammation, rendering neonatal pups resistant to disease. We also examine the lineage relationship between monocyte subsets, reviewing data that suggests classical monocytes are a precursor for non-classical monocytes, and the alternative possibility that separate progenitors exist for each subset. Although a precursor-product relationship between classical and non-classical monocytes might exist in certain environments, we argue that they may also arise from separate progenitors, which is evident by sustained Ly6cLo non-classical monocyte expansion when Ly6cHi monocytes are absent. An improved understanding of monocyte subsets and their developmental trajectories during perinatal hepatic inflammation will provide insight into how therapies directed at controlling monocyte function may help alleviate the devastating consequences of diseases like BA.","PeriodicalId":15473,"journal":{"name":"Journal of clinical & cellular immunology","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85666570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The relative abundance of monocyte subsets determines susceptibility to perinatal hepatic inflammation.","authors":"Sarah Mohamedaly,&nbsp;Anas Alkhani,&nbsp;Amar Nijagal","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The devastating consequences of perinatal liver inflammation contribute to a pressing need to develop therapeutics for the diseases that underly this condition. Biliary atresia (BA) is a perinatal inflammatory disease of the liver that results in obliterative cholangiopathy and rapidly progresses to liver failure, requiring transplantation. The ability to develop targeted therapies requires an understanding of the immune mechanisms that mitigate perinatal liver inflammation. This article reviews our recent findings demonstrating that in a murine model of perinatal hepatic inflammation, Ly6c^Lo non-classical monocytes express a pro-reparative transcriptomic profile and that the relative abundance of Ly6c^Lo monocytes promotes resolution of perinatal liver inflammation, rendering neonatal pups resistant to disease. We also examine the lineage relationship between monocyte subsets, reviewing data that suggests classical monocytes are a precursor for non-classical monocytes, and the alternative possibility that separate progenitors exist for each subset. Although a precursor-product relationship between classical and non-classical monocytes might exist in certain environments, we argue that they may also arise from separate progenitors, which is evident by sustained Ly6c^Lo non-classical monocyte expansion when Ly6c^Hi monocytes are absent. An improved understanding of monocyte subsets and their developmental trajectories during perinatal hepatic inflammation will provide insight into how therapies directed at controlling monocyte function may help alleviate the devastating consequences of diseases like BA.</p>","PeriodicalId":15473,"journal":{"name":"Journal of clinical & cellular immunology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9603689/pdf/nihms-1800188.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40434320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromatin Dynamics and Genetic Variation Combine to Regulate Innate Immune Memory.","authors":"Jennifer Kim,&nbsp;Annie Vogel Ciernia","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Recent work by Ciernia et al. (2020) identified how genetic and epigenetic mechanisms interact to regulate innate immune memory in bone marrow derived macrophages. The authors examined the BTBR strain, a naturally occurring mouse model of Autism Spectrum Disorder (ASD) that captures the complex genetics, behavioral and immune dysregulation found in the human disorder. Immune cell cultures from the BTBR strain compared to the standard C57 showed hyper-responsive immune gene expression that was linked to altered chromatin accessibility at sites with genetic differences between the strains. Together, findings from this work demonstrated that multiple levels of gene regulation likely dictate the formation of innate immune memory and are likely disrupted in immune cells in ASD. Future work will be needed to extend these findings to immune gene regulation in the brain and how changes in immune function are related to abnormal behaviors in brain disorders.</p>","PeriodicalId":15473,"journal":{"name":"Journal of clinical & cellular immunology","volume":"11 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8294664/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39210644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Platelet Glycoprotein iib/iiia (leu 33 pro) Polymorphism and its Role in Recurrent Early Pregnancy Loss","authors":"S. Sadek, D. E. Kaffash, M. Mahdy, O. A. Ellatif","doi":"10.35248/2155-9899.20.11:593","DOIUrl":"https://doi.org/10.35248/2155-9899.20.11:593","url":null,"abstract":"The aim of the work was to evaluate the role of maternal platelet glycoprotein IIb/IIIa polymorphism A2 in early recurrent pregnancy loss. Pregnancy loss (RPL) is one of the most frustrating and difficult areas in reproductive medicine because the etiology is often unknown and there are few evidence-based diagnostic and treatment strategies. The study was carried out on twenty-five non pregnant patients who had at least two consecutive early pregnancy losses with age ranging from twenty to thirty-seven years. The study also included a control group for the molecular part of the study of twenty-five non pregnant women.","PeriodicalId":15473,"journal":{"name":"Journal of clinical & cellular immunology","volume":"49 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91061337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intravenous versus Subcutaneous Immunoglobulin in Primary Immunodeficiency: Real-World Evaluation of Safety, Efficacy, and Patient Perceptions","authors":"B. Geng, F. Piracha, N. Rashid, M. Rigas","doi":"10.35248/2155-9899.20.11:589","DOIUrl":"https://doi.org/10.35248/2155-9899.20.11:589","url":null,"abstract":"Background: Patients with primary immunodeficiency disorders (PIDD) typically require life-long immunoglobulin (IG) replacement therapy. There are two routes of IG administration: intravenous (IVIG) and subcutaneous (SCIG). To better understand routes of IG administration in the real-world, use in a home infusion setting was evaluated. Objective: To evaluate the safety, efficacy, and perceived responses in PIDD patients on IVIG versus SCIG in a realworld, home infusion setting. Methods: Retrospective data were collected from 2010 to 2018 from a national home infusion pharmacy of PIDD patients receiving IVIG or SCIG therapy for at least 6 months with evaluations for safety, efficacy, and patient perception of response. Results: A total of 149 patients were identified for analysis: IVIG (n=84) and SCIG (n=65). Overall, patients in the SCIG group had higher rates of local adverse reactions, while patients receiving IVIG had higher rates of systemic adverse reactions. Both SCIG and IVIG were effective as the majority of patients had ≤ 1 infection or hospital visit within the study period. However, patients in the SCIG group had fewer hospital visits and lower rates of infections overall. Patients receiving SCIG also perceived a faster speed of response. Conclusion: SCIG infusions are safe, efficacious, and well tolerated when compared to IVIG, providing PIDD patients with an alternative route of IG administration. Notably, hospital visits and infection rates were significantly reduced in patients receiving SCIG. The overall findings of this study contribute to growing evidence that demonstrates the benefits of SCIG in adult and pediatric patients with PIDD.","PeriodicalId":15473,"journal":{"name":"Journal of clinical & cellular immunology","volume":"8 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87100712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increased Plasma Cells and Decreased B-cells in Tumor Infiltrating Lymphocytes are Associated with Worse Survival in Lung Adenocarcinomas.","authors":"Hee Eun Lee,&nbsp;Lei Luo,&nbsp;Trynda Kroneman,&nbsp;Marie R Passow,&nbsp;Kristina M Del Rosario,&nbsp;Michael R Christensen,&nbsp;Mary E Francis,&nbsp;John W O'Shaughnessy,&nbsp;Anthony J Blahnik,&nbsp;Ping Yang,&nbsp;Eunhee S Yi","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Introduction: </strong>Clinical significance of tumor-infiltrating plasma cells and B-cells in lung adenocarcinoma is not well known.</p><p><strong>Methods: </strong>CD3, CD20 and MUM1 immunostains were performed on representative tumor blocks selected from 120 consecutive lung adenocarcinoma cases treated by surgical resection at Mayo Clinic Rochester. CD3⁺ T-cells, CD20⁺ B-cells, and MUM1⁺ plasma cells were enumerated separately in the intraepithelial (IE) compartment and the stroma (ST) by digital image analyses using whole sections. Measured tumor-infiltrating plasma cells and B-cells were correlated with patient's overall survival (OS) using Cox proportional hazards analysis.</p><p><strong>Results: </strong>Median age of patients was 69 years (range, 46-91 years) and 52 were male. Median numbers (interquartile range) of CD20⁺ B-cells per 1mm² of tumor area (IE plus ST) and IE compartment within tumor area were 590 (224-1276) and 101 (38-109), respectively; the corresponding numbers of MUM1⁺ plasma cells were 298 (180-605), and 67 (22-145), respectively. The proportion of MUM1⁺ plasma cell among all TILs (MUM1⁺ cells/[CD3⁺ cells +CD20⁺ cells+MUM1⁺ cells] × 100) ranged 1%-59% (median13%) in the tumor area and showed a significant association with OS by univariate Cox analysis (negative correlation with hazard ratio (HR)=12.50 [95% confidence interval (CI), 1.75-89.27]). There was a significant association between IE CD20⁺ B-cells and the patient's OS in univariate analysis (positive correlation with HR=0.81 [95% CI, 0.68-0.96]). Both parameters remained significant by multivariate analysis.</p><p><strong>Conclusion: </strong>High plasma cell % among TILs in the tumor area and low IE B-cell count were associated with worse prognosis in lung adenocarcinoma patients.</p>","PeriodicalId":15473,"journal":{"name":"Journal of clinical & cellular immunology","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7286600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38032060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in Clinical & Cellular Immunology","authors":"A. Asif","doi":"10.35248/2155-9899.20.11:","DOIUrl":"https://doi.org/10.35248/2155-9899.20.11:","url":null,"abstract":"Journal of Clinical & Cellular Immunology is an open access journal which is an essential reading for scholars in all areas of immunological studies. The journal publishes papers describing original work in all areas of immunology including immunochemistry, imaging,   cellular and molecular immunology, mathematical modelling, immunogenetics, transplantation immunology, allergy, clinical immunology and immunological therapies, cancer immunology, physiological functioning of the immune system in states of both health and disease, malfunctions of the immune system in immunological disorders and therapies (hypersensitivities, autoimmune diseases, transplant rejection, immune deficiency), the chemical, physical and physiological characteristics of the components of the immune system in vivo, in situ, and in vitro,  are of prime interest.","PeriodicalId":15473,"journal":{"name":"Journal of clinical & cellular immunology","volume":"1 1","pages":"1-1"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73019707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}