Journal of Clinical Pathology最新文献_第9页

S100 protein is commonly expressed in neuroendocrine tumours of major and minor ampulla. S100 蛋白通常在大肠和小肠的神经内分泌肿瘤中表达。

IF 3.4 4区医学

Journal of Clinical Pathology Pub Date : 2024-06-07 DOI: 10.1136/jcp-2024-209658

Alessandro Vanoli, Frediano Inzani, Paola Parente, Luca Albarello, Matteo Fassan, Federica Grillo, Alessia Messina, Angela Carlino, Silvia Uccella, Paola Spaggiari, Stefano La Rosa, Guido Rindi

引用次数: 0

Ingested foreign bodies mimicking inflammatory bowel disease. 模仿炎症性肠病的误食异物。

IF 3.4 4区医学

Journal of Clinical Pathology Pub Date : 2024-06-05 DOI: 10.1136/jcp-2024-209531

Chenxu Shi, Shariq Raza, Andrew Tieniber, Pak Chau, Franz Fogt

引用次数: 0

Invasion risk of cutaneous squamous cell carcinoma in situ by histological subtype: a retrospective cohort study 按组织学亚型划分的皮肤鳞状细胞原位癌侵袭风险：一项回顾性队列研究

IF 3.4 4区医学

Journal of Clinical Pathology Pub Date : 2024-06-04 DOI: 10.1136/jcp-2024-209608

Danielle K Stamer, Joshua Goldsmith, Laura E Dodge, Steven R Tahan

{"title":"Invasion risk of cutaneous squamous cell carcinoma in situ by histological subtype: a retrospective cohort study","authors":"Danielle K Stamer, Joshua Goldsmith, Laura E Dodge, Steven R Tahan","doi":"10.1136/jcp-2024-209608","DOIUrl":"https://doi.org/10.1136/jcp-2024-209608","url":null,"abstract":"Aims Cutaneous squamous cell carcinoma in situ (SCCis) can be classified histopathologically into four subtypes: full-thickness (FT), hypertrophic actinic keratosis (HAK), Bowenoid, and acantholytic types. 3%–5% of SCCis lesions progress to invasive squamous cell carcinoma (iSCC), however progression risk by subtype has not been assessed. Aim one of this study is to quantitatively assess the risk of iSCC associated with each histological subtype of SCCis. Aim two is to evaluate if the histological grade of iSCC differs among subtypes of the associated SCCis. Methods The pathology information system at our institution was queried for cutaneous SCCis cases with and without associated iSCC from 2020 to 2022. The study group consisted of 65 cases of SCCis with associated iSCC and control group 65 randomly selected cases of SCCis without invasion. For each case SCCis subtype was classified as FT, HAK, Bowenoid or acantholytic type. iSCCs were classified as low grade if well to moderately differentiated (LG) and high grade (HG) if moderately to poorly differentiated. Results iSCC was most often associated with HAK-type SCCis, followed by acantholytic and FT-type SCCis, with Bowenoid type rarely associated with iSCC. 41% (14/34) of iSCCs associated with HAK-type SCCis were HG compared with 84% (21/25) for FT-type SCCis. Conclusions iSCC is most often associated with HAK-type SCCis, followed by acantholytic and FT-types, and rarely with Bowenoid type. HG invasive SCC is most often associated with FT-type, and LG with HAK-type SCCis. Stratifying SCCis by subtype can inform clinical management. All data relevant to the study are included in the article or uploaded as supplementary information.","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141258025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ALK-rearranged mesenchymal neoplasms: a clinicopathological and molecular study of eight additional cases of an emerging group of tyrosine kinase fusion mesenchymal tumours ALK重排间充质肿瘤：对新出现的一组酪氨酸激酶融合间充质肿瘤中另外八例病例的临床病理学和分子研究

IF 3.4 4区医学

Journal of Clinical Pathology Pub Date : 2024-06-04 DOI: 10.1136/jcp-2024-209521

Ming Zhao, Jing Song, Xiaona Yin, Jiayun Xu, Xiaodong Teng, Jian Wang

{"title":"ALK-rearranged mesenchymal neoplasms: a clinicopathological and molecular study of eight additional cases of an emerging group of tyrosine kinase fusion mesenchymal tumours","authors":"Ming Zhao, Jing Song, Xiaona Yin, Jiayun Xu, Xiaodong Teng, Jian Wang","doi":"10.1136/jcp-2024-209521","DOIUrl":"https://doi.org/10.1136/jcp-2024-209521","url":null,"abstract":"Aims Mesenchymal neoplasms characterised by ALK fusions mainly include inflammatory myofibroblastic tumour (IMT) and epithelioid fibrous histiocytoma (EFH). Most recently, ALK -rearranged mesenchymal tumours that are not IMT or EFH have been reported. Our aim is to further characterise eight such neoplasms, with a detailed clinicopathological, immunohistochemical and molecular analysis. Methods Clinicopathological features were assessed and partner agnostic targeted RNA-sequencing on clinically validated platforms was performed. Results The patients consisted of seven males and one female with a median age of 47 years (28 –59 years). The tumours ranged in size from 2.0 to 10.0 cm (mean=3.0 cm) and involved superficial and deep soft tissue (n=6) and visceral locations (n=2). Of the seven patients with follow-up (9–130 months), two developed distant metastases and five had no disease recurrence or metastasis. The tumours demonstrated diverse architectures and variable cellularity and cellular morphologies. The main constitutive cells appeared in elongated spindled in three, primitive to ovoid in two and round to epithelioid in three cases. We expanded the histopathological spectrum to include mildly to moderately cellular spindled to stellate cells in a multinodular growth in a prominent myxoid and vascularised stroma (n=2). All tumours expressed ALK(D5F3); seven were positive for S100 protein and six were positive for CD34. By fluorescence in situ hybridisation, ALK rearrangement was identified in all eight tumours. ALK fusion partners were identified by RNA-sequencing in all cases, including previously reported: EML4 (n=3) , DCTN (n=1) , CLIP1 (n=1) and PLEKHH2 (n=1), and also two novel fusion partners: TKT (n=1) and MMP2 (n=1). Conclusions Our study expands the clinicopathological and molecular spectrum of ALK -rearranged mesenchymal neoplasms. Data are available on reasonable request.","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141258091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Attitudes of UK pathologists and judicial officers towards medicolegal view and grant examinations: a cross-sectional mixed-methods study. 英国病理学家和司法官员对医学法律观点和授予检查的态度：一项横断面混合方法研究。

IF 3.4 4区医学

Journal of Clinical Pathology Pub Date : 2024-05-31 DOI: 10.1136/jcp-2024-209413

Jacob Foster, David Sadler, Sam Taylor

{"title":"Attitudes of UK pathologists and judicial officers towards medicolegal view and grant examinations: a cross-sectional mixed-methods study.","authors":"Jacob Foster, David Sadler, Sam Taylor","doi":"10.1136/jcp-2024-209413","DOIUrl":"https://doi.org/10.1136/jcp-2024-209413","url":null,"abstract":"Aims: Despite the 1988 'Dundee Initiative', which maximised the use of view and grant examinations to reduce the invasive forensic autopsy rate in Tayside, the view and grant itself remains controversial. This is the first study to measure attitudes towards view and grants, applying the Theory of Planned Behaviour to investigate what attitudes are held, the reasons behind them and their association with deciding the scope of postmortem examinations.Methods: A mixed-methods cross-sectional study examined 62 UK pathologists, coroners and procurators fiscal using an online questionnaire. Participants were asked their demographics and attitudes towards view and grants before allocating five fictitious reportable deaths to either view and grant or invasive forensic autopsy (both in ideal and real world conditions), explaining their decisions using free-text.Results: Participants held both positive and negative attitudes towards view and grants, and most were relatively strong and ambivalent. Attitudes predicted respondents' decisions to favour view and grant or invasive forensic autopsy in all ideal world scenarios, but no real world scenarios. There were significant differences in attitudes and decisions when comparing pathologists and judicial officers, and respondents working in Coroner and Fiscal systems. Thematic analysis was conducted on free-text responses.Conclusions: Discrepancies between attitudes, and ideal and real world choices suggest that what respondents wanted to do did not necessarily translate to what they would actually do in the scenarios tested. Applying concepts of attitudes, norms and perceived control can help to understand decision-making by death investigators, and why some jurisdictions favour more invasive procedures.","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141183791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between parathyroid adenoma size, weight and histological subtypes with perioperative serum levels. 甲状旁腺腺瘤的大小、重量和组织学亚型与围手术期血清水平的关系。

IF 3.4 4区医学

Journal of Clinical Pathology Pub Date : 2024-05-31 DOI: 10.1136/jcp-2023-209340

Fred Chuang, Shu Kay Ng, Roger Khan, Alfred King-Yin Lam

{"title":"Relationship between parathyroid adenoma size, weight and histological subtypes with perioperative serum levels.","authors":"Fred Chuang, Shu Kay Ng, Roger Khan, Alfred King-Yin Lam","doi":"10.1136/jcp-2023-209340","DOIUrl":"https://doi.org/10.1136/jcp-2023-209340","url":null,"abstract":"Aims: This study aims to identify associations between parathyroid adenoma (PTA) characteristics (histology, weight and size) with the change in parathyroid hormone (PTH) and calcium levels.Methods: A historical cohort study was conducted on adult patients with solitary PTA removed in the Gold Coast Health Precinct, Australia, between 2017 and 2022.Results: PTA weight is correlated with the change in day 1 PTH level (r=0.26, p=0.036), the change in day 1 corrected calcium level (r=0.20, p=0.033), and the change in follow-up corrected calcium level (r=0.47, p<0.001). The largest dimension (size) of PTA is also correlated with the change in day 1 PTH (r=0.30, p=0.011) and the change in follow-up corrected calcium level (r=0.40, p<0.001). Adjusted for age and gender, a statistically significant negative correlation was found between day 1 PTH level and adenoma size, resulting in a 0.5% change in size for every percentage change in PTH level (equating to a 5.0% increase in variance explained, p=0.038). Similarly, a negative correlation was identified in day 1 corrected calcium levels and weight, with a 4.7% change in weight for every percentage of change in day 1 corrected calcium level (an increase of 5.6% variance explained, p=0.010). In addition, a negative correlation was identified, where every 3.1% change in size (an increase of 17.4% variance explained, p<0.001) and 7.6% change in weight (an increase of 22.7% variance explained, p<0.001) was seen with every percentage change in follow-up corrected calcium levels. Clear-cell PTA had the most significant percentage fall in day 1 corrected calcium levels compared with other PTA subtypes (p=0.007).Conclusions: Preoperative calcium and PTH levels correlate with PTA weight and size. The degree of change in postoperative corrected calcium levels behaved differently in the clear-cell subtype.","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141183792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and clinical outcomes of germline variants among patients with myeloid neoplasms. 髓系肿瘤患者种系变异的患病率和临床结果。

IF 3.4 4区医学

Journal of Clinical Pathology Pub Date : 2024-05-22 DOI: 10.1136/jcp-2023-209264

Sunisa Kongkiatkamon, Pimjai Niparuck, Thanawat Rattanathammethee, Sirorat Kobbuaklee, Amornchai Suksusut, Kitsada Wudhikarn, Chupong Ittiwut, Wanna Chetruengchai, Suporn Chuncharunee, Udomsak Bunworasate, Kanya Suphapeetiporn, Ponlapat Rojnuckarin, Chantana Polprasert

{"title":"Prevalence and clinical outcomes of germline variants among patients with myeloid neoplasms.","authors":"Sunisa Kongkiatkamon, Pimjai Niparuck, Thanawat Rattanathammethee, Sirorat Kobbuaklee, Amornchai Suksusut, Kitsada Wudhikarn, Chupong Ittiwut, Wanna Chetruengchai, Suporn Chuncharunee, Udomsak Bunworasate, Kanya Suphapeetiporn, Ponlapat Rojnuckarin, Chantana Polprasert","doi":"10.1136/jcp-2023-209264","DOIUrl":"https://doi.org/10.1136/jcp-2023-209264","url":null,"abstract":"Aims: Myeloid neoplasms (MNs) with germline predisposition have been recognised as a distinct entity. Emerging evidence suggests that sporadic myelodysplastic syndromes may also harbour undetected germline predispositions. We investigated germline alterations in a cohort of 122 adult Thai MNs.Methods: MN patients were recruited and tested for germline variants using deep targeted next-generation sequencing. The germline variant was filtered using American College of Medical Genetics classifications and then evaluated for the association with clinical characteristics and outcomes.Results: Our findings revealed pathogenic/likely pathogenic germline alterations in 12 (10%) of the patients. These germline lesions were commonly found in the DNA damage response pathway (n=6, 50%). We also identified novel deleterious FANCA A1219GfsTer59 variants in two patients diagnosed with secondary acute myeloid leukaemia (sAML) from aplastic anaemia and AML with myelodysplasia related. Among sAML, individuals with germline mutations had inferior overall survival compared with those with wild-type alleles (2 months vs 12 months) with HR 4.7 (95% CI 1.0 to 20), p=0.037. Therefore, the presence of pathogenic or likely pathogenic mutations may be linked to inferior survival outcomes.Conclusions: Our study highlighted that the prevalence of germline predisposition in Southeast Asian populations is comparable to that in Caucasians. This underscores the importance of germline genetic testing within the Asian population.","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141081560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peripheral blood smear and bone marrow findings in spontaneous tumour lysis syndrome associated with haematolymphoid malignancies. 与血淋巴细胞恶性肿瘤相关的自发性肿瘤溶解综合征的外周血涂片和骨髓检查结果。

IF 3.4 4区医学

Journal of Clinical Pathology Pub Date : 2024-05-20 DOI: 10.1136/jcp-2024-209456

Muthu Sudalaimuthu, Ronit Juthani, Vishva Babu, Rakhee Kar, Debdatta Basu

引用次数: 0

Spatial resolution of renal amyloid deposits through MALDI-MSI: a combined digital and molecular approach to monoclonal gammopathies. 通过 MALDI-MSI 对肾脏淀粉样蛋白沉积进行空间分辨：一种针对单克隆丙种球蛋白病的数字与分子相结合的方法。

IF 3.4 4区医学

Journal of Clinical Pathology Pub Date : 2024-05-17 DOI: 10.1136/jcp-2023-208790

Greta Bindi, Andrew Smith, Glenda Oliveira, Albino Eccher, Simona Vatrano, Federico Alberici, Giorgio Cazzaniga, Stefania Galimberti, Giulia Capitoli, Fulvio Magni, Fabio Pagni, Vincenzo L'Imperio

{"title":"Spatial resolution of renal amyloid deposits through MALDI-MSI: a combined digital and molecular approach to monoclonal gammopathies.","authors":"Greta Bindi, Andrew Smith, Glenda Oliveira, Albino Eccher, Simona Vatrano, Federico Alberici, Giorgio Cazzaniga, Stefania Galimberti, Giulia Capitoli, Fulvio Magni, Fabio Pagni, Vincenzo L'Imperio","doi":"10.1136/jcp-2023-208790","DOIUrl":"10.1136/jcp-2023-208790","url":null,"abstract":"Aims: Identification and characterisation of monoclonal gammopathies of renal significance (MGRS) is critical for therapeutic purposes. Amyloidosis represents one of the most common forms of MGRS, and renal biopsy remains the gold standard for their classification, although mass spectrometry has shown greater sensitivity in this area.Methods: In the present study, a new in situ proteomic technique, matrix-assisted laser desorption/ionisation mass spectrometry imaging (MALDI-MSI), is investigated as an alternative to conventional laser capture microdissection MS for the characterisation of amyloids. MALDI-MSI was performed on 16 cases (3 lambda light chain amyloidosis (AL), 3 AL kappa, 3 serum amyloid A amyloidosis (SAA), 2 lambda light chain deposition disease (LCDD), 2 challenging amyloid cases and 3 controls). Analysis began with regions of interest labelled by the pathologist, and then automatic segmentation was performed.Results: MALDI-MSI correctly identified and typed cases with known amyloid type (AL kappa, AL lambda and SAA). A 'restricted fingerprint' for amyloid detection composed of apolipoprotein E, serum amyloid protein and apolipoprotein A1 showed the best automatic segmentation performance (area under the curve >0.7).Conclusions: MALDI-MSI correctly assigned minimal/challenging cases of amyloidosis to the correct type (AL lambda) and identified lambda light chains in LCDD cases, highlighting the promising role of MALDI-MSI for amyloid typing.","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10816817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical, pathological, genetics and intratumoural immune milieu of micropapillary carcinoma of the colon. 结肠微乳头状癌的临床、病理、遗传学和瘤内免疫环境。

IF 3.4 4区医学

Journal of Clinical Pathology Pub Date : 2024-05-17 DOI: 10.1136/jcp-2023-208895

Vikram Deshpande, Soo Hyun Lee, Andrew Crabbe, Amaya Pankaj, Azfar Neyaz, Yuho Ono, Steffen Rickelt, Swati Sonal, Cristina R Ferrone, David T Ting, Deepa Patil, Omer Yilmaz, David Berger, Osman Yilmaz

{"title":"Clinical, pathological, genetics and intratumoural immune milieu of micropapillary carcinoma of the colon.","authors":"Vikram Deshpande, Soo Hyun Lee, Andrew Crabbe, Amaya Pankaj, Azfar Neyaz, Yuho Ono, Steffen Rickelt, Swati Sonal, Cristina R Ferrone, David T Ting, Deepa Patil, Omer Yilmaz, David Berger, Osman Yilmaz","doi":"10.1136/jcp-2023-208895","DOIUrl":"10.1136/jcp-2023-208895","url":null,"abstract":"Aim: Micropapillary carcinoma (MPC) is a recognised WHO variant of colonic carcinoma (CC), although little is known about its prognosis, immune microenvironment and molecular alterations. We investigated its clinical, pathological and immunological characteristics.Methods: We assessed 903 consecutive CCs and used the WHO definition to identify MPC. We recorded serrated and mucinous differentiation and mismatch repair (MMR) status. We performed immunohistochemistry and quantification on tissue microarrays for HLA class I/II proteins, beta-2-microglobulin (B2MG), CD8, CD163, LAG3, PD-L1, FoxP3, PD-L1and BRAF V600E.Results: We classified 8.6% (N=78) of CC as MPC. Relative to non-MPC, MPC was more often high grade (p=0.03) and showed serrated morphology (p<0.01); however, we found no association with extramural venous invasion (p=0.41) and American Joint Committee on Cancer stage (p=0.95). MPCs showed lower numbers of CD8 positive lymphocytes (p<0.01), lower tumour cell B2MG expression (p=0.04) and lower tumour cell PD-L1 expression (p<0.01). There was no difference in HLA class I/II, LAG3, FOXP3, CD163 and PD-L1 positive histiocytes. There was no association with MMR status or BRAF V600E relative to non-MPC. MPC was not associated with decreased disease-specific survival (p=0.36).Conclusion: MPCs are associated with high-grade differentiation and a less active immune microenvironment than non-MPC. MPC is not associated with inferior disease-specific survival.","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9557282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0