Journal of Clinical Pathology最新文献

{"title":"MTAP loss in gallbladder carcinoma: frequency, heterogeneity and expression in precursor lesions.","authors":"Valentina Angerilli, Jessica Gasparello, Monia Niero, Giacomo Zanus, Matteo Fassan","doi":"10.1136/jcp-2026-210781","DOIUrl":"https://doi.org/10.1136/jcp-2026-210781","url":null,"abstract":"","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147856513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"National Cancer Plan for England has landed: what does it mean for NHS histopathology?","authors":"David Quane, Runjan Chetty","doi":"10.1136/jcp-2026-210768","DOIUrl":"https://doi.org/10.1136/jcp-2026-210768","url":null,"abstract":"","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TCRbeta1/TCRbeta2 (TRBC1/TRBC2) antibody pair for determining T-cell monotypia as a surrogate for clonality in formalin-fixed paraffin-embedded material.","authors":"Anuradha Kaistha, Jinlong J Situ, Shelley Evans, Margaret Ashton-Key, Graham Ogg, Elizabeth Soilleux","doi":"10.1136/jcp-2026-210684","DOIUrl":"https://doi.org/10.1136/jcp-2026-210684","url":null,"abstract":"<p><strong>Aim: </strong>T-cell lymphomas are often histologically indistinguishable from benign T-cell infiltrates. Clonality testing is frequently required for diagnosis. It lacks the spatial context and is slow and expensive, relying on complex multiplexed PCR reactions, interpreted by scientists or pathologists with specialist molecular training. We set out to make monoclonal antibodies to develop a novel immunohistochemical test for T-cell lymphoma, analogous to the kappa/lambda assay for B-cell and plasma cell neoplasms.</p><p><strong>Methods: </strong>We developed a pair of highly specific monoclonal antibodies against the two alternatively used but very similar T-cell receptor β constant regions, TCRβ1 and TCRβ2 (encoded by the TRBC1 and TRBC2 gene segments). We demonstrate the feasibility of immunohistochemical detection of TCRβ1 and TCRβ2 in formalin-fixed, paraffin-embedded tissue as a novel diagnostic strategy for T-cell lymphomas.</p><p><strong>Results: </strong>Single immunostaining results are presented for 13 T-cell lymphomas and 8 benign T-cell populations, together with illustrative examples of TCRβ1/2 double immunostaining. Finally, we show that this single immunostaining is amenable to automated cell counting, permitting accurate calculation of the TCRβ2:TCRβ1 ratio.</p><p><strong>Conclusion: </strong>This novel assay can be used in a similar way to the kappa/lambda assay for B-cell and plasma cell neoplasms.</p>","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147815785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phyllodes tumours of the breast: a 20-year institutional series with emphasis on criteria for malignancy.","authors":"Ping Tang, Alessa Aragao, Mia Yeager, Rachel Gordezky, Nolan Donahue, Lora Xu, Xiuzhen Duan, Emad A Rakha, Puay Hoon Tan","doi":"10.1136/jcp-2026-210626","DOIUrl":"https://doi.org/10.1136/jcp-2026-210626","url":null,"abstract":"<p><strong>Background: </strong>Although histological criteria underpin current classification, accurate risk stratification, particularly for borderline and malignant tumours, remains challenging.</p><p><strong>Methods: </strong>We analysed a 20-year institutional series of phyllodes tumours (PTs), integrating clinicopathological data with long-term clinical outcomes. Histological features were extracted from original pathology reports, with slide re-review where archival material was available.</p><p><strong>Results: </strong>Among 111 PTs, 80% were benign, 11% borderline and 9% malignant. The follow-up ranged from 0 to 20 years, with 11.3, 10.3 and 7.6 years for benign, borderline and malignant PT, respectively. Most patients underwent local excision; mastectomy was performed in 22% of malignant PT. Final positive margins were identified in 18% of benign, 8% of borderline and none of the malignant tumours. Adjuvant radiotherapy was administered in 18% of borderline and 67% of malignant PT. Local recurrence occurred in 3%, 11% and 22% of benign, borderline and malignant tumours, respectively. Distant metastases were observed exclusively in malignant PTs (33%, 3/9 cases), two of which were preceded by local recurrence. Notably, two of the three metastatic malignant PTs showed either ≤10 mitoses/10 high-power field or <marked stromal atypia and lack of stromal overgrowth, which are required for diagnosis of malignant PT per WHO 5^th edition recommendation. The low event rate precluded robust multivariable analysis.</p><p><strong>Conclusion: </strong>Our results highlight the biological heterogeneity of malignant PTs and the limitations of rigid histological thresholds. These findings underscore the need for collaborative, multi-institutional prospective studies with standardised tumour sampling and reporting and molecular correlation to refine clinically meaningful risk stratification in PT.</p>","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147815809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pitfalls in variant interpretation: a critical re-evaluation of somatic versus germline classifications.","authors":"Kok-Siong Poon, Kah Weng Lau","doi":"10.1136/jcp-2026-210689","DOIUrl":"https://doi.org/10.1136/jcp-2026-210689","url":null,"abstract":"","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147729159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EBV-positive Burkitt lymphoma arising in a patient with chronic lymphocytic leukaemia/small lymphocytic lymphoma: a case report.","authors":"Chen Chang, Bo-Yu Huang, Ya-Ping Chen, L Jeffrey Medeiros, Kung-Chao Chang","doi":"10.1136/jcp-2025-210274","DOIUrl":"10.1136/jcp-2025-210274","url":null,"abstract":"","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":" ","pages":"351-353"},"PeriodicalIF":2.0,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145091726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute myeloid leukaemia (AML) harbouring <i>KMT2A</i>-PTD: should it be considered as a myelodysplasia-related abnormality?","authors":"Narasimhapriyan Kannan, Aarti Achrekar, Vishram Terse, Vaibhav Gawde, Swapnali Joshi, Prasanna Bhanshe, Shruti Chaudhary, Pratiksha Salunke, Sitaram Ghogale, Nilesh Deshpande, Dhanlaxmi Shetty, Alok Shetty, Sumeet Mirgh, Lingaraj Nayak, Anant Gokarn, Sachin Punatar, Hasmukh Jain, Bhausaheb Bagal, Manju Sengar, Navin Khattry, Sweta Rajpal, Gaurav Chatterjee, Prashant Tembhare, Papagudi G Subramanian, Nikhil Patkar","doi":"10.1136/jcp-2025-210460","DOIUrl":"10.1136/jcp-2025-210460","url":null,"abstract":"<p><strong>Aims: </strong>Although acute myeloid leukaemia (AML), myelodysplasia-related (AML-MR), can be defined solely by molecular abnormalities, legacy studies did not analyse the <i>KMT2A</i> gene. The <i>KMT2A</i>-partial tandem duplication (<i>KMT2A</i>-PTD) is described in myelodysplastic neoplasms (MDS) as well as AML. We describe the clinical, morphological, immunophenotypic and molecular features of AML harbouring <i>KMT2A-</i>PTD.</p><p><strong>Methods: </strong>We studied 802 adult AML patients, for whom next-generation sequencing- based mutation and copy number analysis were available. For the patients harbouring <i>KMT2A</i>-PTD, the immunophenotypic analysis including measurable residual disease (MRD), mutational landscape and the patient outcomes were analysed.</p><p><strong>Results: </strong>We identified 45 patients of de novo and secondary AML harbouring <i>KMT2A</i>-PTD. Morphological dysplasia and immunophenotypic abnormalities, described in MDS, were observed in 35.6% and 40% of de novo cases respectively. Furthermore, 44% of AML with <i>KMT2A</i>-PTD could be diagnosed as AML-MR based on cytogenetics or genomics. We observed progenitor abnormalities, commonly aberrant CD7 (33%) and CD15 (59%), and granulocytic abnormalities like loss of side scatter (50%), asynchronous maturation patterns and loss of CD177 in mature granulocytes. Frequent mutations involving <i>IDH2</i> (30% of which were R172), <i>FLT3</i> (32% each), <i>RUNX1</i> (29%), <i>DNMT3A</i> and <i>U2AF1</i> (24% each) were noted. We also found that more than 25% of patients did not achieve morphological remission, and the remaining 60% were MRD positive. The outcomes of AML with <i>KMT2A</i>-PTD with or without MR-associated abnormalities were similar.</p><p><strong>Conclusions: </strong>AML harbouring <i>KMT2A</i>-PTD frequently displays MR immunophenotypic abnormalities and is frequently associated with AML-MR type mutations. In addition, they have similar outcomes as compared with AML-MR.</p>","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":" ","pages":"325-331"},"PeriodicalIF":2.0,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147443898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pancreatic metastasis from oncocytic thyroid carcinoma: diagnostic challenges across centres.","authors":"Gyanesh Sethi, Elena Papadakou, Karwan Moutasim, Fabian Sipaul, Adrian C Bateman","doi":"10.1136/jcp-2025-210538","DOIUrl":"10.1136/jcp-2025-210538","url":null,"abstract":"","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":" ","pages":"354-355"},"PeriodicalIF":2.0,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145774749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global standards, local realities: addressing disparities in the WHO classification of tumours ('Blue Books').","authors":"Tanvi Jha, Nadeem Tanveer","doi":"10.1136/jcp-2025-210608","DOIUrl":"10.1136/jcp-2025-210608","url":null,"abstract":"","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":" ","pages":"343-344"},"PeriodicalIF":2.0,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146119015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quo Vadis colorectal intramucosal adenocarcinoma?","authors":"Vikram Deshpande, Adrian C Bateman, Munita Bal, Runjan Chetty, Maurice B Loughrey, Jinru Shia, Michael Vieth, Monika Vyas, Yoh Zen","doi":"10.1136/jcp-2025-210255","DOIUrl":"10.1136/jcp-2025-210255","url":null,"abstract":"<p><p>The classification and clinical implications of colorectal intramucosal adenocarcinoma remain controversial. Given the increasing frequency of diagnosis through colorectal cancer screening programmes, a reassessment of terminology and its impacts is necessary. This paper critically examines the diagnostic criteria, biological behaviour and clinical consequences of labelling these lesions as colorectal intramucosal carcinoma. While intramucosal adenocarcinoma exhibits cytological and architectural atypia beyond high-grade dysplasia, it remains confined to the mucosa and has minimal metastatic potential, with rare documented exceptions. Conversely, the use of carcinoma terminology has been associated with potential overtreatment, including unnecessary surgical resection, increased patient anxiety and financial burdens such as insurance complications. We explore geographic variations in classification and analyse the impact of terminology shifts. We propose a standardised framework that restricts the term intramucosal adenocarcinoma to intramucosal lesions exhibiting tumour budding or poorly differentiated clusters, signet ring cells, desmoplasia, vascular invasion, mucinous differentiation or features of neuroendocrine carcinoma, while reclassifying adenomas with cribriform architecture and complex glands as high-grade dysplasia. This nomenclature shift aims to reduce overtreatment, align with current oncologic understanding and ensure optimal patient care and communication.</p>","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":" ","pages":"291-298"},"PeriodicalIF":2.0,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146219890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}