Journal of Clinical Periodontology最新文献

{"title":"Parkin Acetylation-Mediated Mitophagy Orchestrates Periodontal Ligament Stem Cell Osteogenesis and Bone Regeneration During Ageing.","authors":"Kehan Zhang,Xiangyao Wang,Yuxiao Zhang,Yuanyuan Li,Yaxin Wu,Gaoshaer Nuerlan,Qilin Li,Jing Mao,Shiqiang Gong,Yan Liu","doi":"10.1111/jcpe.70031","DOIUrl":"https://doi.org/10.1111/jcpe.70031","url":null,"abstract":"AIMTo investigate the functional significance of mitophagy in age-related osteogenic decline and the underlying mechanisms using in vivo and in vitro models.MATERIALS AND METHODSAn alveolar bone defect model in aged mice and a serial passaging-induced ageing model of human periodontal ligament stem cells (PDLSCs) were established. Osteogenic potential in mice was assessed by micro-CT, immunofluorescence, immunohistochemical analyses and histological staining. Osteogenic differentiation, mitochondrial function and mitophagy in PDLSCs were assessed using molecular techniques, cytochemical assays and imaging approaches. HDAC2-Parkin interactions and Parkin acetylation status were examined by mass spectrometry and immunoprecipitation to uncover key mechanisms.RESULTSSenescent PDLSCs exhibited impaired mitophagy and osteogenic capacity. Enhancing mitophagy restored osteogenesis of senescent PDLSCs and bone regeneration in aged mice. Mechanistically, HDAC2-mediated deacetylation of Parkin suppressed mitophagy and osteogenesis during ageing.CONCLUSIONActivation of mitophagy reverses age-associated declines in osteogenic function, highlighting mitophagy as a therapeutic target for enhancing bone repair in the ageing population.","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"15 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145008713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Interplay Between Lifestyle and Oral/Faecal Microbial Profiles Among Periodontal Disease Patients: A Cross-Sectional Study.","authors":"Marcella Costa Ribeiro,Ana Paula Vieira Colombo,Adriana Miranda de Oliveira,Talita Gomes Baêta Lourenço,Heitor Marques Honório,Ellen Cristini de Freitas,Michel Reis Messora,Flávia Aparecida Chaves Furlaneto","doi":"10.1111/jcpe.70029","DOIUrl":"https://doi.org/10.1111/jcpe.70029","url":null,"abstract":"AIMTo characterise periodontal and faecal microbiomes of individuals with periodontal health (PH) and diseases, and evaluate associations with periodontal, sociodemographic, anthropometric, nutritional and lifestyle factors.MATERIALS AND METHODSDental biofilm and faecal samples from individuals (n = 24/group) with PH, gingivitis (GG) and periodontitis (PE) were sequenced (16S rRNA). Anthropometric data and questionnaires on demographics, lifestyle, diet and intestinal habits were collected. Data were statistically analysed (p < 0.05).RESULTSGG and PE groups showed higher age, BMI, waist/abdominal circumferences and trans-fat intake and lower selenium and vitamin E intake compared to PH. Individuals with PE had higher hip circumference and lower income, education and intake of iron as well as vitamins A and B9. PE microbiomes (oral and faecal) showed distinct compositions, with the highest number of unique oral species. Faecal richness was lower in PE and GG compared to PH. Specific microbial taxa correlated with periodontal status and host factors.CONCLUSIONPeriodontal and faecal microbiomes vary across periodontal conditions. Discriminant analysis classified 77% of individuals by periodontal status, with key markers for PE including older age, poor dietary quality and distinct microbial oral and faecal signatures. These findings highlight the role of clinical, dietary and microbial factors in periodontal disease profiling.","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"51 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145008716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteome‐Guided Drug Target Discovery for Periodontitis","authors":"Zoheir Alayash, Sebastian‐Edgar Baumeister, Birte Holtfreter, Thomas Kocher, Hansjörg Baurecht, Benjamin Ehmke, Daniel Hagenfeld, Stefan Lars Reckelkamm, Michael Nolde","doi":"10.1111/jcpe.70032","DOIUrl":"https://doi.org/10.1111/jcpe.70032","url":null,"abstract":"Background and ObjectivePeriodontitis is a chronic inflammatory disease driven by immune dysfunction and microbial imbalance. This study aims to identify circulating druggable proteins causally linked to the disease.Materials and MethodsWe integrated proteomics data from deCODE genetics with periodontitis genome‐wide association studies (GWAS) from the Million Veteran Program to identify proteins associated with periodontitis. Findings were replicated using GWAS data from the Gene‐Lifestyle Interactions in Dental Endpoints consortium. Causal associations were validated using genetic and statistical methods, and the identified proteins were assessed for biological relevance and druggability.ResultsAmong the 2088 evaluated proteins, three showed robust evidence of causal association with periodontitis: FGF2 (fibroblast growth factor 2) (odds ratio [OR]: 1.06, 95% confidence interval [CI] 1.032–1.082), AZGP1 (zinc‐alpha‐2‐glycoprotein) (OR: 1.12, 95% CI 1.058–1.189) and BTC (betacellulin) (OR: 0.86, 95% CI 0.789–0.942). Replication analysis confirmed associations for 18 proteins, with 16 showing high colocalisation. Further evaluation of drug target databases revealed indirect links between the identified proteins and approved therapies for inflammatory conditions, suggesting potential therapeutic relevance.ConclusionThis study identifies three circulating proteins—FGF2, AZGP1 and BTC—as causally associated with periodontitis, highlighting their potential as therapeutic targets. These results provide a foundation for future research into targeted therapies for periodontitis.","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145002996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral Characteristics in Individuals With PTEN Hamartoma Tumour Syndrome","authors":"Kristian Nevland, Øystein Fardal, Hildegunn Høberg Vetti, Anne Isine Bolstad","doi":"10.1111/jcpe.70005","DOIUrl":"https://doi.org/10.1111/jcpe.70005","url":null,"abstract":"AimTo describe the oral characteristics and evaluate the oral health status of patients with <jats:italic>PTEN</jats:italic> hamartoma tumour syndrome (PHTS).Materials and MethodsThis descriptive cross‐sectional study enrolled participants diagnosed with PHTS at Haukeland University Hospital, Bergen, Norway. A comprehensive oral investigation was performed, including clinical dental and periodontal examination, assessments of gingival overgrowth and palate architecture, intraoral radiographs, orthopantomogram and intraoral optical scanning.ResultsTwenty PHTS patients (13 females), with a median age of 45 years, participated in the study. A median of 25.5 teeth were present, and a DMFT score of 17 and DMFS score of 58 were recorded. Eleven patients (55%) were diagnosed with periodontitis, with seven classified as stage II, grade B, and four as stage IV, grade C. Nineteen patients (95%) had gingival overgrowth; the median clinical and photographic gingival overgrowth score was 2.0 and 40%, respectively, with 45% of the patients requiring treatment for the condition. Mucosal lesions were a universal finding. Palate height index was 52.0. Eight patients had 13 cancer diagnoses in total.ConclusionPHTS patients face significant oral health challenges, including compromised dental and periodontal health, prevalent gingival overgrowth, mucosal lesions and a high‐arched palate. The findings highlight oral features that may facilitate early recognition of PHTS and reinforce the importance of integrating oral health care into patient management.","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"23 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144928603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CCL2‐Driven Inflammation Links Periodontitis to Anxiety","authors":"Li Liang, Zhen Liu, Nan Yang, Yu Xia, Chen Wang, Hui Gao, Ji‐Feng Yu","doi":"10.1111/jcpe.70020","DOIUrl":"https://doi.org/10.1111/jcpe.70020","url":null,"abstract":"AimThis study investigates the association between periodontitis and anxiety, focusing on the role of the chemokine &lt;jats:styled-content style=\"fixed-case\"&gt;CCL2&lt;/jats:styled-content&gt; in mediating this relationship.Materials and MethodsThis study comprises an analytical cross‐sectional study and a preclinical in vivo study. In the analytical cross‐sectional study, anxiety levels were assessed in individuals with periodontitis and healthy controls using the Hamilton Anxiety Rating Scale (&lt;jats:styled-content style=\"fixed-case\"&gt;HAMA&lt;/jats:styled-content&gt;). Blood and periodontal tissue samples were analysed for &lt;jats:styled-content style=\"fixed-case\"&gt;CCL2&lt;/jats:styled-content&gt; levels via &lt;jats:styled-content style=\"fixed-case\"&gt;ELISA&lt;/jats:styled-content&gt; and monocyte counts via flow cytometry. In the preclinical in vivo study, a mouse model of ligature‐induced periodontitis was established. Anxiety‐like behaviours were evaluated using the Open Field Test and Elevated Plus Maze. Blood as well as periodontal and brain tissues were collected to measure &lt;jats:styled-content style=\"fixed-case\"&gt;CCL2&lt;/jats:styled-content&gt; levels and monocyte/macrophage infiltration through &lt;jats:styled-content style=\"fixed-case\"&gt;ELISA&lt;/jats:styled-content&gt; and flow cytometry. Tight junction proteins in periodontal tissues and brain microvessels were analysed via Western blotting and immunofluorescence. Blood–brain barrier (&lt;jats:styled-content style=\"fixed-case\"&gt;BBB)&lt;/jats:styled-content&gt; permeability was assessed using Evans Blue extravasation. A &lt;jats:styled-content style=\"fixed-case\"&gt;CCL2&lt;/jats:styled-content&gt;‐neutralising antibody was administered to assess its effects on anxiety and periodontal pathology.ResultsIn the analytical cross‐sectional study (50 individuals with periodontitis and 50 healthy controls), individuals with periodontitis showed higher anxiety levels, correlating with elevated &lt;jats:styled-content style=\"fixed-case\"&gt;CCL2&lt;/jats:styled-content&gt; levels in blood (56.84 [15.87] pg/&lt;jats:styled-content style=\"fixed-case\"&gt;mL&lt;/jats:styled-content&gt; vs. 19.28 [7.47] pg/&lt;jats:styled-content style=\"fixed-case\"&gt;mL&lt;/jats:styled-content&gt; in controls, &lt;jats:italic&gt;p&lt;/jats:italic&gt; &lt; 0.0001) and periodontal tissues (67.37 [23.10] pg/&lt;jats:styled-content style=\"fixed-case\"&gt;mL&lt;/jats:styled-content&gt; vs. 22.77 [10.21] pg/&lt;jats:styled-content style=\"fixed-case\"&gt;mL&lt;/jats:styled-content&gt; in controls, &lt;jats:italic&gt;p&lt;/jats:italic&gt; &lt; 0.0001), as well as increased monocyte counts (&lt;jats:styled-content style=\"fixed-case\"&gt;CD14&lt;/jats:styled-content&gt;&lt;jats:sup&gt;+&lt;/jats:sup&gt; monocytes in blood: 8.08 [3.01]% vs. 5.28 [1.84]% in controls, &lt;jats:italic&gt;p&lt;/jats:italic&gt; &lt; 0.01). In the preclinical in vivo study (total &lt;jats:italic&gt;n&lt;/jats:italic&gt; = 80 mice, with &lt;jats:italic&gt;n&lt;/jats:italic&gt; = 8 per group in analyses), periodontal inflammation increased &lt;jats:styled-content style=\"fixed-case\"&gt;CCL2&lt;/jats:styled-content&gt; expression in periodontal tissues (125.80 [55.10] pg/&lt;jats:style","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"26 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144923862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Buccal Bone Wall Thickness Dictates the Extent of Vertical Buccal Bone Loss Following Implant Placement: A Preclinical Study","authors":"Jean‐Claude Imber, Andrea Roccuzzo, Alberto Monje, Benjamin Pippenger, Dieter D. Bosshardt, Anton Sculean, Daniel Buser","doi":"10.1111/jcpe.70027","DOIUrl":"https://doi.org/10.1111/jcpe.70027","url":null,"abstract":"AimTo assess buccal vertical bone resorption following implant placement in healed sites with varying buccal bone wall thicknesses.Materials and MethodsIn 11 miniature pigs, three tapered hybrid titanium implants were placed per hemi‐maxilla in healed bone. Sites were randomised into three groups based on buccal bone wall thickness: G1 (&lt; 1.0 mm), G2 (1.0–1.5 mm) and G3 (&gt; 1.5 mm). Animals were euthanised at 24 h and 2, 4 or 8 weeks. Histological and histometric analyses were performed. The primary outcome was the vertical distance from the transition point (TP) between the machined and moderately rough implant surfaces to the first bone‐to‐implant contact (fBIC).ResultsHealing was uneventful. At 2 weeks, all groups showed buccal resorption, although G3 exhibited earlier bone apposition and fewer resorptive signs. By 8 weeks, all G1 implants displayed exposure of the moderately rough surface, while only one implant in G3 showed exposure. TP‐fBIC values at 8 weeks were 0.92 ± 0.63 mm (G1), 0.27 ± 0.54 mm (G2, <jats:italic>p</jats:italic> = 0.041) and −0.16 ± 0.17 mm (G3, <jats:italic>p</jats:italic> = 0.0002). Bone‐to‐implant contact increased over time across all groups.ConclusionThin buccal bone walls (&lt; 1 mm) were associated with greater vertical bone loss and implant surface exposure, whereas thick walls (&gt; 1.5 mm) preserved the buccal bone better and protected the implant surface.","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"29 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144919139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association Between Diabetes and Peri‐Implantitis: Evidence From a Swedish Register‐Based Study","authors":"Anna Trullenque‐Eriksson, Fernando Valentim Bitencourt, Cristiano Tomasi, Tord Berglundh, Jan Derks","doi":"10.1111/jcpe.70023","DOIUrl":"https://doi.org/10.1111/jcpe.70023","url":null,"abstract":"AimTo evaluate the association between diabetes (types 1 and 2) and peri‐implantitis through a register‐based cohort study.MethodsFour groups of individuals with dental implants were identified using multiple Swedish nationwide registers—two groups with diabetes (type 1, T1D; type 2, T2D) and two groups without diabetes (non‐T1D, non‐T2D). Longitudinal data from 2010 to 2020 were analysed. Peri‐implantitis was defined as any registered treatment of peri‐implantitis. Prevalence of peri‐implantitis (<jats:italic>n</jats:italic> = 18,975) was evaluated using covariate‐adjusted logistic regression analyses. Incidence (<jats:italic>n</jats:italic> = 2030) was compared using survival analyses across groups matched by age, gender, education, income and the number of implants, through propensity scores.ResultsPeri‐implantitis was more frequent among those with T1D compared to non‐T1D (21.1% vs. 15.2%; OR 1.46, 95% CI: 1.05–2.04), whereas the prevalence was similar in T2D and non‐T2D (20.5% vs. 18.2%; OR 1.06, 95% CI: 0.98–1.16). The hazard ratios for incident peri‐implantitis were 1.52 (95% CI: 0.96–2.42) and 1.36 (95% CI: 1.02–1.82) for T1D and T2D, respectively.ConclusionsT1D and T2D were associated with a higher risk for peri‐implantitis. While the elevated risk for peri‐implantitis in T1D was particularly apparent in prevalence estimates, the association for T2D was evident mainly in terms of incidence.","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"27 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144915361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Do Systemic Antibiotics Offer Benefits to the Surgical Treatment of Peri‐Implantitis? A Systematic Review With Meta‐Analyses","authors":"Georgios N. Antonoglou, Spyridon N. Papageorgiou, Ana Carrillo de Albornoz, Michael Payer, Andreas Stavropoulos","doi":"10.1111/jcpe.70021","DOIUrl":"https://doi.org/10.1111/jcpe.70021","url":null,"abstract":"AimTo assess the potential benefit of using adjunct systemic antibiotics in surgical peri‐implantitis treatment.Materials and MethodsSix databases were searched (December 2024) for randomised/non‐randomised clinical studies. After duplicate study selection, data extraction and risk‐of‐bias assessment, random‐effects meta‐analyses of odds ratios (ORs) or mean differences (MDs) and their 95% confidence intervals (CIs) were performed, followed by the analysis of certainty of evidence.ResultsSeven studies (three randomised and four non‐randomised, comprising 595 patients and 1388 implants) were included. Systemic antibiotics were associated with greater short‐term treatment success (<jats:italic>n</jats:italic> = 5; OR = 2.33; 95% CI: 1.29–4.21), bone gain (<jats:italic>n</jats:italic> = 4; MD = 0.37 mm; 95% CI: –0.68 to –0.07), increased bone level stability (<jats:italic>n</jats:italic> = 3; OR = 2.73; 95% CI: 1.50–4.99), reduced bleeding on probing (<jats:italic>n</jats:italic> = 6; OR = 0.49; 95% CI: 0.31–0.78), reduced suppuration on probing (<jats:italic>n</jats:italic> = 3; OR = 0.33; 95% CI: 0.18–0.61) and increased gingival recession (<jats:italic>n</jats:italic> = 3; MD = 0.18 mm; 95% CI: 0–0.36 mm) (<jats:italic>p</jats:italic> &lt; 0.05). Systemic antibiotics seem to benefit only implants with modified surfaces (ORs: modified 4.10 vs. turned 0.79), and event that, without long‐term benefits (≥ 3 years). Finally, one trial found that antibiotics probably increased diarrhoea risk.ConclusionsEvidence from randomised/non‐randomised studies seems to indicate that systemic antibiotics benefit surgical peri‐implantitis treatment, in the short term (1–2 years), especially for implants with a modified surface, while data on adverse effects is scarce. No substantial long‐term benefits are seen (≥ 3 years). Uncertainty still exists regarding the potential benefit of systemic antibiotics as adjunct to surgical management of peri‐implantitis.","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"8 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144915364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Collagen Sponges and Gelatine Sponges as Dressing for Palatal Wounds: A Randomised Controlled Clinical Trial","authors":"Jia‐Ping Huang, Yao Jiang, Xiao‐Yuan Cheng, Jing Wang, Yu‐Han Huang, Anna Dai, Shuai Zhou, Wei‐Yi Pan, Shu‐Lei Fu, Yi‐Yu Wang, Pei‐Hui Ding","doi":"10.1111/jcpe.70028","DOIUrl":"https://doi.org/10.1111/jcpe.70028","url":null,"abstract":"AimTo evaluate the clinical outcomes of collagen sponges (CSs) on wound healing following palatal graft harvesting and compare their efficacy with gelatine sponges (GSs).Materials and MethodsThirty‐two participants who had undergone free gingival grafts or de‐epithelialised gingival grafts were randomised into the CS group or the GS group. Wound healing rate was calculated as the percentage of the healed wound area divided by the initial wound area. Wound healing rate and complete epithelialisation were evaluated at 1, 2, 3 and 4 weeks. Postoperative pain was assessed on days 1, 3 and 7. Willingness to repeat graft harvesting, delayed bleeding and aesthetic outcomes were also recorded.ResultsCS group had higher wound healing rates than GS group at 1 and 2 weeks (24.44% ± 6.28% vs. 5.56% ± 2.19%, <jats:italic>p</jats:italic> &lt; 0.01; 91.54% ± 3.20% vs. 75.56% ± 4.77%, <jats:italic>p</jats:italic> &lt; 0.05). Complete epithelialisation was achieved within 2–3 weeks in CS group and within 2–4 weeks in GS group, but no significant difference was found. Postoperative pain in VAS was lower in the CS group on Day 1 compared with the GS group (1.6 ± 0.4 vs. 3.1 ± 0.5, <jats:italic>p</jats:italic> &lt; 0.05). No significant difference could be detected in willingness to repeat graft harvesting, delayed bleeding and aesthetic outcomes.ConclusionsCompared with GS, CS seemed to offer improved early healing and reduced postoperative pain following palatal graft harvesting.Trial RegistrationThis trial was registered in the Chinese Clinical Trial Registry with the registration number ChiCTR2200057221 (<jats:ext-link xmlns:xlink=\"http://www.w3.org/1999/xlink\" xlink:href=\"https://www.chictr.org.cn/showproj.html?proj=154474\">https://www.chictr.org.cn/showproj.html?proj=154474</jats:ext-link>) on March 4, 2022","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"23 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144915362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Profiling the Bacterial Microbiome Across Peri‐Implant Conditions","authors":"Joaquin Espinoza‐Arrue, Marion Arce, Natalia Endo, Anilei Hoare, Nicolas Dutzan, Loreto Abusleme","doi":"10.1111/jcpe.70024","DOIUrl":"https://doi.org/10.1111/jcpe.70024","url":null,"abstract":"AimTo comprehensively characterise the bacterial microbiome in peri‐implant health, peri‐implant mucositis and peri‐implantitis.Materials and MethodsA re‐analysis of raw microbiome data was performed from 15 studies, which were finally selected based on the availability of 16S rRNA sequencing. Reads were pre‐processed using mothur and classified using the HOMD database. A total of 522 samples were analysed to evaluate diversity estimates and bacterial relative abundance, identifying discriminant features via LEfSe, while predictions of functional potential were obtained using PICRUSt2. Bacterial co‐occurrence networks were constructed, and dysbiosis was measured by employing the subgingival microbiome dysbiosis index.ResultsPeri‐implantitis showed higher bacterial diversity compared to health and greater microbial richness than peri‐mucositis. Each clinical condition displayed a distinct community structure and bacterial co‐occurrence networks. The representative species in peri‐implant health were <jats:styled-content style=\"fixed-case\"><jats:italic>Rothia aeria</jats:italic></jats:styled-content>, <jats:styled-content style=\"fixed-case\"><jats:italic>R. dentocariosa</jats:italic></jats:styled-content> and <jats:italic>Veillonella parvula_dispar</jats:italic>. Peri‐mucositis is characterised by <jats:styled-content style=\"fixed-case\"><jats:italic>Leptotrichia hongkongensis</jats:italic></jats:styled-content>, <jats:styled-content style=\"fixed-case\"><jats:italic>L. wadei</jats:italic></jats:styled-content> and <jats:styled-content style=\"fixed-case\"><jats:italic>Fusobacterium nucleatum</jats:italic></jats:styled-content> subsp. <jats:italic>polymorphum</jats:italic>, while peri‐implantitis is defined by <jats:styled-content style=\"fixed-case\"><jats:italic>Porphyromonas gingivalis</jats:italic></jats:styled-content>, <jats:styled-content style=\"fixed-case\"><jats:italic>F. nucleatum</jats:italic></jats:styled-content> subsp. <jats:italic>vincentii</jats:italic> and <jats:italic>Tannerella <jats:styled-content style=\"fixed-case\">forsythia</jats:styled-content></jats:italic>. Peri‐implantitis exhibited enrichment in predicted microbial pathogenesis pathways and greater bacterial dysbiosis.ConclusionsThese results provide deeper insights into the peri‐implant microbiome, identifying key bacterial species, functional processes and interactions that may be crucial to inflammation and destruction during peri‐implant diseases.","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"3 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144905848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}