Macrophage Depletion Reduces Bone Loss and Alters Inflammatory Responses: A Mouse Peri‐Implantitis Model

IF 5.8 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Clinical Periodontology Pub Date : 2025-04-16 DOI:10.1111/jcpe.14120

Ofir Ginesin, Benjamin R. Coyac, Ofri Doppelt‐Flikshtain, Yaniv Mayer, Eran Gabay, Tal Berg, Yotam Bar‐On, Hadar Zigdon‐Giladi

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引用次数: 0

Abstract

AimTo evaluate the impact of macrophage depletion on bone loss and inflammatory responses in a mouse model of peri‐implantitis, assessing macrophage depletion potential as a therapeutic strategy.Materials and MethodsUsing 6‐week‐old male C57BL/6 mice, peri‐implantitis was induced by placing a silk ligature around osteointegrated dental implants. Mice were divided into three groups: Healthy control (Healthy group); peri‐implantitis with liposomal phosphate‐buffered saline (PBS group); and peri‐implantitis with liposomal clodronate for macrophage depletion (CLOD group). Two weeks after ligature placement, micro‐CT, histological and real‐time PCR analyses were performed to assess bone density, leukocyte infiltration and cytokine levels.ResultsThe CLOD group showed a significantly higher bone‐to‐implant contact (74%) and bone volume relative to total volume (79%) compared to the PBS group (53% and 54%, respectively) and Healthy group (64% and 66%, respectively). Histological analysis revealed significantly reduced leukocyte and macrophage counts in the CLOD group. Additionally, TNF‐α and IL‐10 levels were significantly decreased in the CLOD group compared with the PBS group.ConclusionMacrophage depletion effectively reduces bone loss and inflammation in peri‐implantitis. This study highlights targeting macrophages as a promising approach for managing peri‐implantitis, although further research is needed to optimize therapeutic strategies.

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巨噬细胞减少骨质流失和改变炎症反应：小鼠种植体周围炎模型

目的评估巨噬细胞消耗对种植体周围炎小鼠模型骨丢失和炎症反应的影响，评估巨噬细胞消耗作为一种治疗策略的潜力。材料和方法用6周龄雄性C57BL/6小鼠，在骨整合牙种植体周围扎丝诱导种植体周围炎。将小鼠分为三组：健康对照组（健康组）；用磷酸盐缓冲盐水脂质体治疗种植体周围炎（PBS组）；用氯膦酸脂质体治疗巨噬细胞消耗（CLOD组）。结扎后两周，进行显微CT、组织学和实时PCR分析，评估骨密度、白细胞浸润和细胞因子水平。结果与PBS组（分别为53%和54%）和Healthy组（分别为64%和66%）相比，CLOD组的骨与种植体的接触（74%）和骨体积相对于总体积（79%）明显更高。组织学分析显示，CLOD组白细胞和巨噬细胞计数明显减少。此外，与PBS组相比，CLOD组的TNF - α和IL - 10水平显著降低。结论巨噬细胞清除可有效减少种植体周围炎的骨丢失和炎症。这项研究强调了靶向巨噬细胞作为治疗种植体周围炎的一种有希望的方法，尽管需要进一步的研究来优化治疗策略。

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来源期刊

Journal of Clinical Periodontology 医学-牙科与口腔外科

CiteScore

13.30

自引率

10.40%

发文量

175

审稿时长

3-8 weeks

期刊介绍： Journal of Clinical Periodontology was founded by the British, Dutch, French, German, Scandinavian, and Swiss Societies of Periodontology. The aim of the Journal of Clinical Periodontology is to provide the platform for exchange of scientific and clinical progress in the field of Periodontology and allied disciplines, and to do so at the highest possible level. The Journal also aims to facilitate the application of new scientific knowledge to the daily practice of the concerned disciplines and addresses both practicing clinicians and academics. The Journal is the official publication of the European Federation of Periodontology but wishes to retain its international scope. The Journal publishes original contributions of high scientific merit in the fields of periodontology and implant dentistry. Its scope encompasses the physiology and pathology of the periodontium, the tissue integration of dental implants, the biology and the modulation of periodontal and alveolar bone healing and regeneration, diagnosis, epidemiology, prevention and therapy of periodontal disease, the clinical aspects of tooth replacement with dental implants, and the comprehensive rehabilitation of the periodontal patient. Review articles by experts on new developments in basic and applied periodontal science and associated dental disciplines, advances in periodontal or implant techniques and procedures, and case reports which illustrate important new information are also welcome.