Journal of Biomaterials and Tissue Engineering最新文献

{"title":"The Impact of Different Final Apical Preparation Diameters on the Efficiency of Photon-Induced Photoacoustic Streaming (PIPS) in Removing the Smear Layer at the Apical Third","authors":"K. Almadi, M. Alkahtany","doi":"10.1166/jbt.2023.3303","DOIUrl":"https://doi.org/10.1166/jbt.2023.3303","url":null,"abstract":"Aims: Photon-induced Photoacoustic streaming (PIPS) as an adjunct to conventional irrigation (CI) using different final apical preparation diameters on smear layer (SL) Material and method: Clinical crown was removed from forty human mandibular molars standardizing root\u0000 length to 18 mm. Chamber was opened and straight-line access was established Working length was kept 1 mm short of the apex. Teeth were randomly divided into 4 investigated groups based on the final apical diameter. Group-1 (F1+CI+PIPS), Group-2 (F2+CI+PIPS), Group-3 (F3+CI+PIPS) and Group-4\u0000 (F3+CI) (Control). CI protocol (2.5% NaOCl+17% EDTA+2.5% NaOCl+distilled water). SEM magnification of 5000x dentinal tubules’ patency at the apical third of root canals was assessed. ANOVA and Tukey’s HSD test were used for data analysis (p = 0.05) Results: Group-3\u0000 (F3+CI+PIPS displayed the highest mean scores (3.64±0.50) of SL removal from the apical third of the canal. However, Group-1 (F1+CI+PIPS) exhibited the lowest mean scores (1.27±0.47). Intergroup comparison analysis revealed that Group 1 and Group 2 (F2+CI+PIPS) presented the\u0000 lowest and most comparable SL removal (p >0.05) Conclusion: Photon-induced Photoacoustic streaming should be considered as an adjunct therapy to improve the cleanliness of the Smear layer from the apical third.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48494806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-221-3p Modulates Cervical Cancer Cells Proliferation and Invasion After Co-Culture with Bone Marrow Mesenchymal Stem Cells (BMSCs)","authors":"Hongyan Cheng, Tao Jiang","doi":"10.1166/jbt.2023.3292","DOIUrl":"https://doi.org/10.1166/jbt.2023.3292","url":null,"abstract":"Bone marrow-derived mesenchymal stem cells (BMSCs) affect EMT-related factors. miR-221-3p involves in several tumors. However, whether miR-221-3p affects cervical cancer (CC) cells co-cultured with BMSCs is unclear. BMSCs and CC cells were co-cultured, and transfected with miR-221-3p\u0000 inhibitor followed by analysis of miR-221-3p level by real time PCR, cell proliferation, apoptosis activity, E-cadherin and Vimentin level, TGF-β1 secretion by ELISA as well as Smad1 and Smad2 expression. BMSCs upregulated miR-221-3p level in CC cells, increased cell proliferation\u0000 and reduced apoptotic activity along with the decreased expression of EMT, increased TGF-β1 secretion and Smad1 and Smad2 expression (P <0.05). miR-221-3p inhibitor can reduce BMSCs’ effect on CC cells, and reverse the above changes (P <0.05). The co-culture\u0000 of BMSCs promotes CC cell proliferation and invasion. Down-regulating miR-221-3p can change TGF-β1/Smad signaling pathway and affect malignant characteristics of CC cells.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43725903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Fluorescence of Tooth Enamel, Resin Composite, Lithium Disilicate and Monolithic Zirconia Under Thermocyclic Ageing","authors":"M. Alqahtani, Hisham Abushaqqaf, Yazeed Assiry, Ahmed S Almslam, Fahad Al-obaid, Yazeed Alzamel, Ahmed Alhejazi, A. Alanzi","doi":"10.1166/jbt.2023.3284","DOIUrl":"https://doi.org/10.1166/jbt.2023.3284","url":null,"abstract":"The aim was to compare the fluorescence intensity of tooth enamel, resin composite, lithium disilicate, and monolithic zirconia before and after thermocyclic aging. Lithium disilicate (LD) and monolithic Zirconia ceramic samples were fabricated with the CAD-CAM method. Resin composite\u0000 specimens, of shades A1, A2, and A3, were prepared with photo-polymerization and putty mold. Extracted second molar teeth were obtained and sectioned to produce enamel specimens. Fluorescence assessment prior to thermocycling was performed using flurolog-Qm system before and after thermocycling.\u0000 Samples were exposed to thermocycling (TC) for 30,000 cycles in distilled water at 5 °C and 55 °C for 30 s each, with 5 s between the baths. The means and standard deviations were compared using, t-test, analysis of variance, and Tukey–Kramer multiple comparisons test.\u0000 The initial fluorescence intensities before thermocyclic ageing were highest in LD ceramic specimens (42579±817.1). The lowest fluorescence was observed in monolithic Zr specimen 14750±584.7. The reduction in fluorescence intensity after thermocyclic ageing was highest in LD\u0000 specimens, however, the lowest difference was noted in composite A3 specimens. LD ceramic showed significantly high fluorescence among all materials before and after ageing. The fluorescence of the tested materials reduced with ageing and is expected to decrease intraorally with time.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48386717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoy Interleukin-1 Receptor Antagonist on Extracellular Vesicles","authors":"Pingquan Zhang, Sun Yijia, Zirong Huang, Liang Yujie, Weimin Zhu","doi":"10.1166/jbt.2023.3280","DOIUrl":"https://doi.org/10.1166/jbt.2023.3280","url":null,"abstract":"Interleukin-1 (IL-1) is an important inflammatory factor in multi-organ inflammation and tissue damage. Interleukin-1 receptor antagonist (IL-1RA) is a bioactive receptor for IL-1. The interaction of these two—IL-1 and its particular receptor antagonist, IL-1RA—influences\u0000 the propensity and severity of numerous illnesses. Importantly, therapies using IL-1RA have been applied in treatment of human inflammatory diseases like rheumatoid arthritis. In this study, we designed a “decoy” cell-derived nanocapsule, which uses stably-expressed HEK293T cells\u0000 to display the IL-1RA on the outer surface of exosomes to act as a decoy antagonist against IL-1. After preparation, the decoy exosomes were characterized using Western Blot, transmission electron microscopy (TEM), and nanoparticle tracking analysis (NTA) to confirm whether they retained the\u0000 correct size and shape of naturally-occurring exosomes. Results indicated that the IL-1Ra protein was successfully expressed on exosomes that had been secreted by HEK293T cells that were transfected with a pcDNA3.1(+)-IL-1RA-N-termSyntenin recombinant plasmid. This work provides a favorable,\u0000 exosome-based tool for the targeted delivery of IL-1Ra for the treatment of joint inflammatory diseases.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42816523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety Assessments of Electroacupuncture-Mediated Blood–Brain Barrier Opening: An Animal Study","authors":"Hao Wang, Xuqing Mao, Li Ren, Cong-Cong Ma, Yibin Zhao, Lin Gan, Shanshan Zhang, Peng Gong, Yu-bo Lin, Xianming Lin","doi":"10.1166/jbt.2023.3301","DOIUrl":"https://doi.org/10.1166/jbt.2023.3301","url":null,"abstract":"The presence of the Blood–Brain barrier (BBB) makes it difficult for therapeutic agents to reach the brain to treat brain diseases. As an emerging treatment, electroacupuncture (EA)-mediated BBB opening can temporarily increase the permeability of the brain blood vessels to increase\u0000 the concentration of the therapeutic agent in the brain parenchyma, which offers great clinical benefits. Recent studies have suggested that BBB opening with excessive exposure levels may cause tissue/cell damage and short-term behavioral changes. Here, we investigated whether the EA-mediated\u0000 BBB opening cause serious adverse events in the normal rat brain tissue and motor behavior, such as brain tissue damage, histopathologic alteration, or aggravated behavioral changes. EA was performed on the accupoint GV26 and GV20. Evans Blue Assay (EBA) and fluorescein isothiocyanate (FITC)–dextran\u0000 Assay were performed to assess the BBB permeability. Immunofluorescence of GFAP-positive cells was marked and Hematoxylin-eosin (HE) Staining was carried out for assessing brain cortex damage. Tunel Assay was conducted to assess cell apoptosis. ELISA was used to measure the contents of TNF-α\u0000 and IL-1β in brain tissue to explore the inflammatory response. The open field test, balance beam test and Y-Maze test were carried out to assess and neural behaviour. EA can induce an increase in BBB permeability on rats, allowing the macromolecular tracer Evans blue (EB) and\u0000 Fluorescein Isothiocyanate (FITC)–Dextran to enter the brain. Histological analysis indicated that there was no obvious red blood cell leakage and a cellular apoptotic response in the brain tissue of rats with EA treatment. In addition, EA stimulation at specific parameters did not impair\u0000 the rats’ motor ability, balance and coordination, and short-term spatial learning and memory. Our results suggest that EA can safely and effectively open the BBB in rats without causing brain damage and behavioral memory impairment.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47207326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Different Veneering Techniques on the Fracture Resistance of Bioceramic Lithium Disilicate Ceramics Crowns","authors":"Ali Barakat, Mohammed S. Alomari","doi":"10.1166/jbt.2023.3281","DOIUrl":"https://doi.org/10.1166/jbt.2023.3281","url":null,"abstract":"Aim: The effect of three different veneering techniques (layering, press-over, and CAD/CAM techniques) on the fracture resistance of lithium disilicate crown (LDC). Material and Methods: Thirty lithium disilicate crowns were adhesively cemented on the standardized Epoxy\u0000 die. LDC was fabricated according to the veneering materials and techniques into three groups (n = 10): group (LV) layering veneering technique, group (PV) pressed veneering technique, and group DV (CAD/CAM) technique. The specimen was artificially aged through dynamic loading and thermocycling.\u0000 All specimens were tested for fracture resistance using compressive load. Descriptive statistics of frequency distribution mean and standard deviations were calculated and compared across different groups. ANOVA was used to evaluate the effect of the veneering technique on fracture resistance.\u0000 Results: The highest load was demonstrated in the DV group (1057.26762±97.04401 N) and the lowest load was found in PV group (762.41229±102.56927 N). Similarly, the highest fracture resistance was observed in group DV (14.65171±1.34484 MPa), and the lowest was found\u0000 in group PV group (10.56558±1.42141 MPa). Mean values of maximum loads and fracture resistance in veneers fabricated by digital, pressed, and layer veneering techniques showed a significant difference. Conclusion: The CAD/CAM veneered monolithic lithium disilicate crowns demonstrated\u0000 superior fracture resistance compared to the lithium disilicate crowns fabricated by over-pressing and layering techniques.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48089682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatibility and Mineralization Potential of Myrrh (Commiphora molmol) on Human Bone Marrow-Derived Mesenchymal Stem Cells","authors":"Lamees Alssum, Maha Alghofaily, Mona Elsafadi, Jawahir Abuhaimed, Randa Almadhari, Nouf Alshibani, R. Al-Kattan, Amer Mahmood","doi":"10.1166/jbt.2023.3282","DOIUrl":"https://doi.org/10.1166/jbt.2023.3282","url":null,"abstract":"Background and objectives: Myrrh (Commiphora molmol) is a natural resinous substance derived from the bark of the Commiphora molmol tree, which is native to Eastern Africa and the Arabian Peninsula. It has been used for thousands of years in traditional medicine\u0000 for its well-known antimicrobial, analgesic, and anti-inflammatory properties. Recently, it has gained attention for its potential regenerative medicine applications. The aim of the current study was to evaluate the biocompatibility and mineralization potential of myrrh on human mesenchymal\u0000 stem cells (hMSC). Methods: Myrrh solution (MS) was prepared from commercial organic myrrh resin. The hMSC cell line were exposed to nine different concentrations of MS and viability was assessed using the Alamar Blue assay. The mineralization potential of myrrh was evaluated using\u0000 alkaline phosphatase (ALP) activity assay and Alizarin Red S (ARS) staining. Results: At concentrations lower than 15.6 ug/ml after 7 and 14 days of treatment, cell viability levels were not markedly different from the control indicating low cytotoxic effect of the MS on hMSC. ALP levels\u0000 were higher in the MS experimental groups compared to the control group. The AZR results were consistent with the ALP levels and confirmed that MS promoted hMSC mineralization. Conclusions: These findings confirm the cellular biocompatibility and the mineralization potential of myrrh\u0000 in hMSC cell lines in vitro.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45619376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Calycosin on Airway Inflammation and Airway Remodeling in Allergic Asthma Mouse Model","authors":"Li Huang, Mingjuan Zhang, Jin-rong Xiong","doi":"10.1166/jbt.2023.3291","DOIUrl":"https://doi.org/10.1166/jbt.2023.3291","url":null,"abstract":"Background: This study aimed to construct a TDI-induced mouse model of asthma, and evaluate the potential effects and possible molecular mechanisms of Calycosin on airway inflammation and airway remodeling in mouse model. Material and methods: ELISA method was applied\u0000 to detect the total serum IgE level and the inflammatory cytokine level in the bronchoalveolar lavage fluid. The total number of cells and the proportion of inflammatory cells in BALF were evaluated under an optical microscope. HE was employed to assess and score the infiltration of peritracheal\u0000 and perivascular inflammatory cells in lung tissue, and PAS staining was used to assess the proportion of goblet cells in the airway epithelium and the thickness of airway epithelial reticular basement membrane in each group of mice. WB was used to detect the expressions of HMGB1 and a-SMA\u0000 in cells. Immunofluorescence staining was used to detect the expressions of HMGB1 and a-SMA in 16HBEs. Results: The airway hyperresponsiveness of the Calycosin TDI asthma mice decreased, the inflammatory factors in BALF and the total serum IgE levels decreased, the airway epithelial\u0000 goblet cell metaplasia and the thickness of the airway epithelial reticular basement membrane were improved, thus reducing the up-regulation of HMGB1 and a-SMA expression of 16HBES induced by TDI-HSA. Conclusion: In our study, in the TDI-induced mouse model of asthma, the administration\u0000 of drug to inhibit the activation of AKT can reduce airway inflammation and airway remodeling. These findings have enriched the current understanding of Calycosin and provided a basis for future research. However, there are also some limitations: How does TDI activate the AKT signaling pathway?\u0000 After the activation of the AKT pathway, the mechanism by which the expressions of HMGB1, α-SMA and Collagen-I were up-regulated has not been fully elucidated.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43781798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Raf-1/ERK Signaling Pathway in Estradiol and Propranolol in the Intervention of Xenograft Hemangioma In Vivo","authors":"Yanpeng Xu, Jiahuan Li, Song Yu, Yan Chen, Zhixu He","doi":"10.1166/jbt.2023.3285","DOIUrl":"https://doi.org/10.1166/jbt.2023.3285","url":null,"abstract":"The pathogenesis and the mechanism of orally administered propranolol in the treatment of hemangioma are unclear. In this study, we evaluated the changes of xenograft hemangioma in nude mice after intervention with estradiol and propranolol. Raf-1 and p-ERK expression in xenograft hemangiomas\u0000 was assessed to evaluate their role in hemangioma proliferation and regression after treatment. A hemangioma xenograft model in nude mice was established. The successful xenograft specimens were selected and then randomized into control group, estradiol group and propranolol group. At the\u0000 date of injection, and on day 7 and 21 after injection, the morphological changes of xenograft hemangiomas were visually characterized and imaged by light microscopy. The distribution and expression Raf-1 and p-ERK protein was determined by immunohistochemical detection. In control group,\u0000 the xenografts increased gradually in volume, had a soft texture and their colors gradually turned red with observation of proliferation of endothelial cells and a capillary lumen that contained monolayer endothelial cells. In Estradiol group, the xenografts grew fast and increased significantly\u0000 in volume, had a soft texture and their colors were dark red with a hyperplasia of endothelial cells, irregular volume, and deranged and compact endothelial cells. More capillary lumens and sinuses were also seen. Raf-1 and p-ERK expression in estradiol group was significantly increased (P\u0000 < 0.05). In Propranolol group, the xenografts volume decreased, had a soft texture, and their colors turned gradually white with decreased number of proliferative endothelial cells. The vascular lumens, composed of endothelial cells, were larger, and some of them disappeared and were replaced\u0000 by fibrous connective tissue and vascular adipose tissue. Raf-1 and p-ERK expression in propranolol group was lower than estradiol and control group (P < 0.05). In conclusion, Raf-1/ERK signaling pathway may be involved in hemangioma. Estrogen and propranolol may regulate the proliferation\u0000 or regression of hemangioma through Raf-1/ERK signaling pathway.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42010580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uncovering Potential Novel Biomarkers in Immunoglobulin-Resistant Kawasaki Disease Using Bioinformatics Analysis","authors":"Luoyi Hu","doi":"10.1166/jbt.2023.3278","DOIUrl":"https://doi.org/10.1166/jbt.2023.3278","url":null,"abstract":"Intravenous immunoglobulin (IVIG)-resistant Kawasaki disease (KD) is a complicated disorder, which can induce multiple-system damage. The pathogenic factor inducing KD remains unclear. The present study focused on identifying potential novel biomarkers for IVIG-resistant KD using integrated\u0000 analyses. Eight IVIG-resistant KD samples and twelve IVIG-sensitive KD samples were included in the GSE18606 dataset. A Linear Model for Microarray Data (LIMMA) identified 504 differentially expressed genes (DEGs), An IVIG-resistant KD sample was compared with an IVIG-sensitive KD sample to\u0000 identify 17 modules through weighted gene co-expression network analysis (WGCNA). A common gene (CG) is the intersection of DEGs and genes in the most significant module. Analysis of functional enrichment revealed that CGs were mainly enriched in TNF signaling pathways and NF-kappa B signaling\u0000 pathways. Ten of these genes were selected as hub genes because of their high degree of connectivity (KLF1, AHSP, HBQ1, HBA2, HBA1, EPB42, GYPB, UBB, KRT1 and BPIFB2).","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47271697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}