{"title":"miR-221-3p对骨髓间充质干细胞(BMSCs)共培养后宫颈癌细胞增殖和侵袭的调控作用","authors":"Hongyan Cheng, Tao Jiang","doi":"10.1166/jbt.2023.3292","DOIUrl":null,"url":null,"abstract":"Bone marrow-derived mesenchymal stem cells (BMSCs) affect EMT-related factors. miR-221-3p involves in several tumors. However, whether miR-221-3p affects cervical cancer (CC) cells co-cultured with BMSCs is unclear. BMSCs and CC cells were co-cultured, and transfected with miR-221-3p\n inhibitor followed by analysis of miR-221-3p level by real time PCR, cell proliferation, apoptosis activity, E-cadherin and Vimentin level, TGF-β1 secretion by ELISA as well as Smad1 and Smad2 expression. BMSCs upregulated miR-221-3p level in CC cells, increased cell proliferation\n and reduced apoptotic activity along with the decreased expression of EMT, increased TGF-β1 secretion and Smad1 and Smad2 expression (P <0.05). miR-221-3p inhibitor can reduce BMSCs’ effect on CC cells, and reverse the above changes (P <0.05). The co-culture\n of BMSCs promotes CC cell proliferation and invasion. Down-regulating miR-221-3p can change TGF-β1/Smad signaling pathway and affect malignant characteristics of CC cells.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1000,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"miR-221-3p Modulates Cervical Cancer Cells Proliferation and Invasion After Co-Culture with Bone Marrow Mesenchymal Stem Cells (BMSCs)\",\"authors\":\"Hongyan Cheng, Tao Jiang\",\"doi\":\"10.1166/jbt.2023.3292\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Bone marrow-derived mesenchymal stem cells (BMSCs) affect EMT-related factors. miR-221-3p involves in several tumors. However, whether miR-221-3p affects cervical cancer (CC) cells co-cultured with BMSCs is unclear. BMSCs and CC cells were co-cultured, and transfected with miR-221-3p\\n inhibitor followed by analysis of miR-221-3p level by real time PCR, cell proliferation, apoptosis activity, E-cadherin and Vimentin level, TGF-β1 secretion by ELISA as well as Smad1 and Smad2 expression. BMSCs upregulated miR-221-3p level in CC cells, increased cell proliferation\\n and reduced apoptotic activity along with the decreased expression of EMT, increased TGF-β1 secretion and Smad1 and Smad2 expression (P <0.05). miR-221-3p inhibitor can reduce BMSCs’ effect on CC cells, and reverse the above changes (P <0.05). The co-culture\\n of BMSCs promotes CC cell proliferation and invasion. Down-regulating miR-221-3p can change TGF-β1/Smad signaling pathway and affect malignant characteristics of CC cells.\",\"PeriodicalId\":15300,\"journal\":{\"name\":\"Journal of Biomaterials and Tissue Engineering\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.1000,\"publicationDate\":\"2023-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Biomaterials and Tissue Engineering\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1166/jbt.2023.3292\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Biomaterials and Tissue Engineering","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1166/jbt.2023.3292","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
骨髓间充质干细胞(BMSCs)影响emt相关因素。miR-221-3p参与多种肿瘤。然而,miR-221-3p是否影响与骨髓间充质干细胞共培养的宫颈癌(CC)细胞尚不清楚。将BMSCs与CC细胞共培养,转染miR-221-3p抑制剂,real - time PCR检测miR-221-3p水平、细胞增殖、凋亡活性、E-cadherin、Vimentin水平、ELISA检测TGF-β1分泌、Smad1、Smad2表达。BMSCs上调CC细胞中miR-221-3p水平,增加细胞增殖,降低细胞凋亡活性,同时降低EMT表达,增加TGF-β1分泌,增加Smad1、Smad2表达(P <0.05)。miR-221-3p抑制剂可降低BMSCs对CC细胞的作用,逆转上述变化(P <0.05)。骨髓间充质干细胞的共培养促进了CC细胞的增殖和侵袭。下调miR-221-3p可改变TGF-β1/Smad信号通路,影响CC细胞的恶性特征。
miR-221-3p Modulates Cervical Cancer Cells Proliferation and Invasion After Co-Culture with Bone Marrow Mesenchymal Stem Cells (BMSCs)
Bone marrow-derived mesenchymal stem cells (BMSCs) affect EMT-related factors. miR-221-3p involves in several tumors. However, whether miR-221-3p affects cervical cancer (CC) cells co-cultured with BMSCs is unclear. BMSCs and CC cells were co-cultured, and transfected with miR-221-3p
inhibitor followed by analysis of miR-221-3p level by real time PCR, cell proliferation, apoptosis activity, E-cadherin and Vimentin level, TGF-β1 secretion by ELISA as well as Smad1 and Smad2 expression. BMSCs upregulated miR-221-3p level in CC cells, increased cell proliferation
and reduced apoptotic activity along with the decreased expression of EMT, increased TGF-β1 secretion and Smad1 and Smad2 expression (P <0.05). miR-221-3p inhibitor can reduce BMSCs’ effect on CC cells, and reverse the above changes (P <0.05). The co-culture
of BMSCs promotes CC cell proliferation and invasion. Down-regulating miR-221-3p can change TGF-β1/Smad signaling pathway and affect malignant characteristics of CC cells.