Journal of Biomaterials and Tissue Engineering最新文献_第10页

Role of Magnesium in Tumor Microenvironment and Underlying Molecular Mechanisms 镁在肿瘤微环境中的作用及其分子机制

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-02-01 DOI: 10.1166/jbt.2023.3247

Xiao-Jiao Nie, Yonghua Shi, Tingting Cui, Chen Lin

引用次数: 0

Bone Marrow Mesenchymal Stem Cells (BMSC) Homing with Chemotaxis Transplantation of Stromal Cell-Derived Factor 1a Promotes the Corneal Damage Repair 骨髓间充质干细胞归巢与基质细胞衍生因子1a趋化移植促进角膜损伤修复

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-01-01 DOI: 10.1166/jbt.2023.3215

Xia Zhang, Jing Dai, Ying Q Lin, H. Su, Xi Luo

引用次数: 0

miR-141-Modified Bone Marrow Mesenchymal Stem Cells (BMSCs) Inhibits the Progression of Severe Acute Pancreatitis mir -141修饰的骨髓间充质干细胞(BMSCs)抑制严重急性胰腺炎的进展

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-01-01 DOI: 10.1166/jbt.2023.3190

Wei Su, Yinshan Wu, Huijun Zheng, Xiuliu Guo, Binbin Feng, F. Guo

{"title":"miR-141-Modified Bone Marrow Mesenchymal Stem Cells (BMSCs) Inhibits the Progression of Severe Acute Pancreatitis","authors":"Wei Su, Yinshan Wu, Huijun Zheng, Xiuliu Guo, Binbin Feng, F. Guo","doi":"10.1166/jbt.2023.3190","DOIUrl":"https://doi.org/10.1166/jbt.2023.3190","url":null,"abstract":"The therapeutic effects of bone marrow mesenchymal stem cells (BMSCs) on severe acute pancreatitis (SAP) and miRNAs are currently the research hotspots. This study intends to explore the potential impact of miR-141-modified BMSCs on SAP. After establishment of rat model of SAP, the\u0000 animals were grouped into control group, model group, BMSCs group, miR-141 group, positive control group, and PI3K/mTOR signaling agonist group (agonist group) followed by analysis of miR-141 expression by RT-qPCR and the expression of serum amylase, IL-6, TNF-α, TAP, PI3K, mTOR,\u0000 and LC3-II by Western blot and ELISA. miR-141 was significantly up-regulated in the miR-141-modified BMSCs group (p > 0.05). The contents of serum amylase, IL-6, TNF-α, and TAP was increased in SAP rats and decreased after BMSC treatment (p > 0.05). The increased\u0000 autophagy flux in the rats with SAT was reduced upon treatment with BMSCs and autophagy flux was decreased in miR-141 group but increased in positive control group. The model and positive control group presented highest expression of LC3-II, p-PI3K and p-mTOR, followed by BMSCs group and miR-141\u0000 group (p < 0.05). In conclusion, miR-141-modified BMSCs decrease the phosphorylation of PI3K and mTOR to inhibit PI3K/mTOR signaling activity and downregulate LC3-II protein to inhibit autophagy, thereby ameliorating the development of SAP, indicating that miR-141 might be a therapeutic\u0000 target for SAP.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42349800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxymatrine Inhibits Malignant Behaviors of Breast Cancer Cells by Inhibiting miR-188 Expression to Up-Regulate Phosphatase and Tensin Homolog (PTEN) 氧化苦参碱通过抑制miR-188表达上调磷酸酯酶和Tensin同源物（PTEN）抑制癌症细胞恶性行为

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-01-01 DOI: 10.1166/jbt.2023.3220

Xiaobo Wang, Yili Hu, Diandian Chen, Le Cheng, Lili Yu, Quanjun Yang

{"title":"Oxymatrine Inhibits Malignant Behaviors of Breast Cancer Cells by Inhibiting miR-188 Expression to Up-Regulate Phosphatase and Tensin Homolog (PTEN)","authors":"Xiaobo Wang, Yili Hu, Diandian Chen, Le Cheng, Lili Yu, Quanjun Yang","doi":"10.1166/jbt.2023.3220","DOIUrl":"https://doi.org/10.1166/jbt.2023.3220","url":null,"abstract":"Oxymatrine has been applied to anti-cancer therapies for various cancers. The present study aimed to investigate the potential impact of miR-188 on breast cancer (BC) cell progression and underlying mechanism. After establishment of a rat model of BC, rats were administered with oxymatrine\u0000 (4 mg/kg, 8 mg/kg), Xihuang pill (XH) (positive control), and miR-188 mimic (1 mg/kg) followed by analysis of tumor growth, the expression of miR-188, MMP-9, MMP-2, and PTEN, and BC cell behaviors. Oxymatrine significantly decreased tumor incidence and reduced tumor mass (p<0.05)\u0000 with 8 mg/kg intervention group and positive control group exhibiting higher tumor inhibition rate (p<0.05). In addition, oxymatrine or XH effectively reduced cell proliferation, invasion and migration rate. Of note, compared to 4 mg/kg oxymatrine, 8 mg/kg oxymatrine and XH showed\u0000 more significantly inhibitory effects on BC cells. Moreover, oxymatrine or XH significantly downregulated miR-188, MMP-9, and MMP-2 and upregulated PTEN. Mechanically, PTEN was indicated as the target of miR-188 with specific binding between them. In conclusion, Oxymatrine inhibits BC cell\u0000 behaviors through down-regulation of miR-188 to increase PTEN expression. This study might provide a new basis for the management of BC.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42618377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of RAP1GDS1 in the Inhibition of Glut1 Expression by Catechins in Tongue Squamous Carcinoma Cells RAP1GDS1在儿茶素抑制舌鳞癌细胞Glut1表达中的作用

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-01-01 DOI: 10.1166/jbt.2023.3237

W. Su, Pan Liu, Yang Zhang, Zhongcheng Gong, Hua-rong Zhao

{"title":"The Role of RAP1GDS1 in the Inhibition of Glut1 Expression by Catechins in Tongue Squamous Carcinoma Cells","authors":"W. Su, Pan Liu, Yang Zhang, Zhongcheng Gong, Hua-rong Zhao","doi":"10.1166/jbt.2023.3237","DOIUrl":"https://doi.org/10.1166/jbt.2023.3237","url":null,"abstract":"Background: The paper aimed to investigate the role of Glut1 in the inhibition of the invasion and migration capabilities of tongue squamous carcinoma cell by Catechin. Materials and Methods: Transwell assay and scratch test were applied to measure the effect of Catechin\u0000 on the invasion and migration of the cells, and WB to detect the expression of each protein. Overexpressed lentivirus was used to up-regulate Glut1 in tongue squamous carcinoma cells, detecting whether the process can be reversed. After silencing RAP1GDS1 in tongue squamous carcinoma cells\u0000 by RNAi technology, the effect of Catechin on Glut1 expression was measured. Results: The results of Transwell assay and scratch test verified that Catechin can inhibit the invasion and migration. Subsequent WB experiments confirmed that Catechin can inhibit the expression of Glut1,\u0000 N-cadherin and Vimentin, while promote the expression of E-cadherin. The up-regulation of Glut1 can significantly reverse the inhibitory effect of Catechin. Silencing of RAP1GDS1 can inhibit the expression of Glut1. However, the up-regulation of Glut1 expression can significantly reverse this\u0000 process. WB result verified the inhibitive effect of Catechin on RAP1GDS1 in tongue squamous carcinoma cells. Conclusion: Catechin affects glycolytic activity by down-regulating Glut1 and induces EMT process inhibition. It is speculated that, through regulating Glut1 by silencing RAP1GDS1.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43618329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Dendrobine on Cardiac Dysfunction in Rats with Myocardial Infarction by Regulating Autophagy 石斛碱通过调节自噬对心肌梗死大鼠心功能障碍的影响

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-01-01 DOI: 10.1166/jbt.2023.3227

Juan Fan, Yi Zhang

{"title":"Effect of Dendrobine on Cardiac Dysfunction in Rats with Myocardial Infarction by Regulating Autophagy","authors":"Juan Fan, Yi Zhang","doi":"10.1166/jbt.2023.3227","DOIUrl":"https://doi.org/10.1166/jbt.2023.3227","url":null,"abstract":"Aim: To observe Dendrobine (Den) on rats with post-myocardial infarction cardiac dysfunction and mechanism. Materials: Dividing 27 rats as Sham, Model and Den groups, rats treated with two weeks of drug had their cardiac function and structure measured by ultrasound; their myocardial\u0000 pathological changes observed by HE and Masson staining and observe apoptosis cell number by TUNEL staining; their serum activities of LDH and CK-MB detected by ELISA; myocardial autophagy protein expressions detected by WB and immunohistochemistry. Results: Model group displayed decreased\u0000 cardiac function levels, enlarged area of myocardial fibrosis, more serum activities of LDH and CK-MB, increased myocardial tissue structural damage and apoptosis cell number, downregulated LAMP2 expression, and up-regulated expressions of Beclin1, LC3-II/LC3-I rate, and P62. To rat victims\u0000 of myocardial infarction, Den improved cardiac function, reduced area of myocardial fibrosis, compromised activities of serum LDH and CK-MB, and relieved damage in myocardial structure, decreased apoptosis cell number in myocardial tissue, up-regulated the expressions of Beclin1, LAMP2 and\u0000 LC3-II, and down-regulated P62 to promote the autophagy in myocardium damaged by myocardial infarction. Conclusion: Den alleviates post-myocardial infarction cardiac dysfunction through improvement of autophagosomes formation and autophagic flux via Beclin1/LAMP2 pathway.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43339450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-129-5p Induces Cell Apoptosis and Inhibits Inflammation by Inflammatory Signaling to Alleviate Spinal Cord Injury (SCI) miR-129-5p诱导细胞凋亡并通过炎症信号抑制炎症以减轻脊髓损伤（SCI）

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-01-01 DOI: 10.1166/jbt.2023.3197

Zhe-Ming Song, Yuwei Gao, Yuhao Zhao, Xiaofei Feng, Zhenjie Zhao, Wenji Wang

{"title":"miR-129-5p Induces Cell Apoptosis and Inhibits Inflammation by Inflammatory Signaling to Alleviate Spinal Cord Injury (SCI)","authors":"Zhe-Ming Song, Yuwei Gao, Yuhao Zhao, Xiaofei Feng, Zhenjie Zhao, Wenji Wang","doi":"10.1166/jbt.2023.3197","DOIUrl":"https://doi.org/10.1166/jbt.2023.3197","url":null,"abstract":"Spinal cord injury (SCI) is a spinal cord nerve dysfunction secondary to trauma. Until now, still no appropriate drug with unclear etiology. Therefore, it is to develop effective SCI treatment methods. Herein, we intended to detect the impact of miR-129-5p in SCI After establishment\u0000 of a mouse SCI model, the animals received intrathecal injection of agomir-miR-129-5p or normal saline. Then, the miR-129-5p’s effect was evaluated by assessing motor function, spinal cord tissue edema, apoptosis and inflammation of mice upon treatments and potential targeted pathways\u0000 of the miRNA were detected. Overexpressed miR-129-5p facilitated the wound healing with less spare tissue and water content. Additionally, overexpressed miR-129-5p suppressed the in vivo inflammation with decreased apoptotic rate of neurons. As SCI induced increased expression of HMGB1,\u0000 TLR4, and NF-κB in tissues, but the presence of miR-129-5p reversed the expressions. Collectively, this study elucidate miR-129-5p significantly improves inflammatory response and apoptosis, thereby improving the condition of SCI. These findings might provide a new theory for\u0000 the disorder, and promote the research progress on the disease.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":"352 5","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41296453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In silico Identification of Potential Human Acetylcholinesterase Inhibitors from the Nigella sativa Phytochemicals 黑草植物化学物质中潜在人乙酰胆碱酯酶抑制剂的计算机鉴定

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-01-01 DOI: 10.1166/jbt.2023.3221

Hani S. H. Mohammed Ali

引用次数: 0

miR-134 Up-Regulates Matrix Metalloproteinase 9 (MMP9) in Chronic Sinusitis miR-134在慢性鼻窦炎中上调基质金属蛋白酶9（MMP9）

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-01-01 DOI: 10.1166/jbt.2023.3206

W. Cao, Yuanzhou Liu, Yandan Chen

引用次数: 0

Bone Marrow Mesenchymal Stem Cells (BMSCs) Retard the Aggressive Migrating and Invading Activity of Non-Small Cell Lung Cancer Cells 骨髓间充质干细胞延缓非小细胞肺癌细胞的侵袭性迁移和侵袭活性

IF 0.1 4区医学

Journal of Biomaterials and Tissue Engineering Pub Date : 2023-01-01 DOI: 10.1166/jbt.2023.3219

Ying Kang, P. Zhang, Kai Xiong, Yuanguo Wang

{"title":"Bone Marrow Mesenchymal Stem Cells (BMSCs) Retard the Aggressive Migrating and Invading Activity of Non-Small Cell Lung Cancer Cells","authors":"Ying Kang, P. Zhang, Kai Xiong, Yuanguo Wang","doi":"10.1166/jbt.2023.3219","DOIUrl":"https://doi.org/10.1166/jbt.2023.3219","url":null,"abstract":"Non-small cell lung cancer (NSCLC) is a common malignancy worldwide. miR-119-3p is down regulated in many cancers. Nonetheless, the modulatory mechanism of bone marrow mesenchymal stem cells (BMSCs) in NSCLC is unclear. Our research aims to dissect the activity of BMSCs on NSCLC and\u0000 underlying mechanisms. After isolation and identification, BMSCs were co-cultured with NSCLC cells, which were transfected with miR-119-3p mimics followed by analysis of expression of miR-119-3p and tumor aggressiveness-related proteins, cell invasion/migration and survival. A significantly\u0000 reduced miR-119-3p level was found in NSCLC cell lines. miR-119-3p mimics inhibited the proliferative, migrating and invasive behaviors of NSCLC cells. Co-culture with BMSCs enhanced miR-119-3p expression in NSCLC cells, thereby suppressing NSCLC cell biological behaviors. Simultaneously,\u0000 the EMT process was markedly restrained, as indicated by an elevated level of E-cadherin but diminished levels of Vimetnin, N-cadherin and Snail. In conclusion, BMSCs can interfere with the EMT process of NSCLC via up-regulatingmiR-119-3p, thereby retarding the aggressive migration and invasive\u0000 capability of NSCLC cells.","PeriodicalId":15300,"journal":{"name":"Journal of Biomaterials and Tissue Engineering","volume":" ","pages":""},"PeriodicalIF":0.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45725387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0